MyristoylCoA:Protein N-Myristoyltransferase
Supplementary material to
N-terminal N-Myristoylation of Proteins:
I. Refinement of the Sequence Motif and its Taxon-specific Differences
II. Prediction of Substrate Proteins from Amino Acid Sequence


For our sequence search for NMT homologues the NMT1 of Arabidopsis thaliana was chosen as primary query sequence since it is evolutionary in the middle of the two big outlying clusters (fungi, mammals) according to our previous analysis.

The following search results are accessible via HTML-pages:

PSI-BLAST (nr) 50 hits (related taxonomy report)

TBLASTN (nr) 70 hits (related taxonomy report)

TBLASTN (est) 525 hits (related taxonomy report)

TBLASTN (gss) 39 hits (related taxonomy report)

TBLASTN (htgs) 35 hits (related taxonomy report)

We provide the alignment of the NMTs in NR database (including 2 potential NMT homologues from entomopoxviri) and further below a complete alignment (including ESTs) of sequences homologous to experimentally characterized NMTs from 64 different organisms. 

Alignment of the 21 NMT homologues in nr-database plus the two viral sequences:


Alignment of sequences from 64 organisms (including putative NMTs from ESTs):



Alignments were generated with ClustalX (Thompson et al. 1997; Matrix: Gonnet, Gap open penalty: 10, Gap extension penalty: 0.2). 

Accession numbers of the sequences:  

Saccharomyces cerevisiae NR P14743
Candida albicans NR P30418
Candida glabrata NR O74234
Schizosaccharomyces pombe NR O43010
Cryptococcus neoformans NR P34809
Histoplasma capsulatum NR P34763
Aspergillus fumigatus NR Q9UVX3
Arabidopsis thaliana1 NR AAF60968
Arabidopsis thaliana2 NR AAK49038
Leishmania major NR AAG38102
Plasmodium falciparum NR AAF18461
Drosophila melanogaster NR AAD27855
Caenorhabditis elegans NR P46548
Bos taurus1 NR P31717
Bos taurus2 NR Q9N181
Mus musculus2 NR O70311
Homo sapiens2 NR O60551
Mus musculus1 NR O70310
Homo sapiens1 NR P30419
Brassica oleracea NR AAF19802
Guillardia theta NR NP_113498
Amsacta moorei entomopoxvirus NR AAG02925
Melanoplus sanguinipes entomopoxvirus NR NP_048143
Giardia intestinalis HTGS AC075683(+1) AC041267(+3) AC088637(+1)
Saccharomyces klyveri GSS AZ124646(+2)
Trypanosoma brucei GSS AL490833(-3)
Trypanosoma cruzi GSS/EST AZ051049(+1) AI069625(+3)
Tetraodon nigroviridis GSS AL166863(+3) AL170798(+2) AL186230(-3) AL319620(-2) AL319620(-3)
Saccharomyces servazzi GSS AL404522(+3)
Saccharomyces unisporus GSS AZ930605(+1)
Entamoeba histolytica GSS AZ672640(-3) AZ685745(+1)
Plasmodium vivax GSS AZ572682(-3)
Plasmodium berghei GSS AZ526668(+3) AZ525157(-1)
Danio rerio EST AW134126(+3) AI444250(+2) AI657581(+2) AI878266(+2)
Pleuronectes americanus EST AW013694(+1)
Sus scrofa EST F14725(+1)
Pneumocystis carinii EST AW334395(-1)
Aspergillus nidulans EST AI211114(+3)
Schistosoma mansoni EST AW017038(+2)
Rattus norvegicus EST BE126380(+3) C06728(+3) BF522952(+2) AI501085(-1)
Strongyloides stercoralis EST BE580365(+3)
Rattus sp EST AW141187(+1)
Meloidogyne javanica EST BG737070(+3)
Ciona intestinalis EST AV671484(+2) AV671935(+2) AV676318(-1)
Bombyx mori EST AV403090(+3)
Aspergillus niger EST BE759691(+1)
Triticum aestivum EST BE402458(+2) BE606254(+3) BE418143(+2)
Gallus gallus EST BG711647(+2)
Glycine max EST AW132453(+2) BF069724(+3) BF070843(+1)
Xenopus laevis EST AW645003(+1) BE026066(+3) AW766461(-1)
Medicago truncatula EST BF648024(+3) AW586887(+3)
Phytophthora infestans EST BE776171(+3)
Ceratopteris richardii EST BE641641(+3)
Pinus taeda EST AW982000(+2) AW290567(+1) AW888213(+3)
Oryza sativa HTGS AP003273(-1)
Zea mays EST BE644280(+1)
Sorghum propinquum EST BF481036(+2)
Hordeum vulgare EST BE194349(+2)
Dictyostelium discoideum EST AU060092(+2) AU034531(+3)
Gossypium hirsutum EST AI726036(+2)
Solanum tuberosum EST BE471614(+2) BG350597(+2)
Beta vulgaris EST BI095908(+2)
Lycopersicon esculentum EST AW649554(+3) AW224043(+2) AI897548(+1) AW036027(+1) AW037713(+3)
Lotus japonicus EST AV409858(+1) AV423721(+3)
Mesembryanthemum crystallinum EST BE131169(-3)
Gossypium arboreum EST BF278989(+1) BF279264(+2) BG446339(+2)
Populus tremula EST BI069814(+1)
Sorghum bicolor EST BG465115(+2)

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