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Comparison: Application of the big-PI fungal predictor on the S.pombe protein list created by de Groot et al., Yeast. 2003 Jul 15;20(9):781-96

Contact:
Birgit Eisenhaber (IMP/Austria)
Georg Schneider (IMP/Austria)
Michael Wildpaner (IMP/Austria)
Frank Eisenhaber (IMP/Austria)

Summary Table:

  Prediction Quality Rate Percentage
1:P 24/33 72.73%
1:S - -
1:S (Positive Total Score) - -
1:S (Negative Total Score) - -
1:I 8/33 24.24%
1:N 1/33 3.03%
Not Predicted 9/33 27.27%
Predicted 24/33 72.73%

Reasons for not predicted proteins are detailed below

N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
1 SPAC1002.13c 417 1:I 393 13.14 -17.42 -4.28 1.993970e-03
2 SPAC1039.11c 995 1:I 979 3.92 -32.72 -28.80 6.415152e-02
3 SPAC11E3.13c 510 1:P 485 20.79 -0.39 20.40 5.277011e-07
4 SPAC1705.03c 421 1:P 398 22.94 -0.01 22.93 1.421282e-07
5 SPAC1786.02 644 1:P 620 17.23 -0.07 17.16 2.415935e-06
6 SPAC17A5.04c 512 1:P 483 14.54 -3.30 11.24 2.612864e-05
7 SPAC19B12.02c 542 1:P 516 20.00 -0.25 19.75 7.278275e-07
8 SPAC19G12.16c 670 1:P 647 14.09 0.00 14.09 8.822658e-06
9 SPAC1F8.02c 226 1:P 199 18.58 -0.35 18.23 1.492079e-06
10 SPAC23D3.14c 581 1:I 549 -2.96 -13.60 -16.56 1.627210e-02
11 SPAC26A3.01 533 1:P 508 18.64 -1.37 17.27 2.304969e-06
12 SPAC27E2.11c 81 1:P 55 18.70 -1.95 16.75 2.898490e-06
13 SPAC27F1.07 450 1:P 423 16.33 -8.36 7.97 8.009654e-05
14 SPAC2E1P3.05c 197 1:I 171 6.27 -60.25 -53.98 3.123216e-01
15 SPAC821.09 1016 1:I 998 2.97 -33.16 -30.19 7.258952e-02
16 SPAPB15E9.01c 1036 1:P 1011 22.93 -0.02 22.91 1.436156e-07
17 SPAPJ760.03c 166 1:I 149 -1.45 -21.29 -22.74 3.491428e-02
18 SPBC16A3.13 774 1:I 750 8.30 -9.50 -1.20 1.007058e-03
19 SPBC1E8.05 317 1:P 287 17.52 -0.68 16.84 2.788149e-06
20 SPBC215.13 534 1:P 508 19.78 -0.34 19.44 8.445572e-07
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
21 SPBC342.03 456 1:P 428 14.51 -0.54 13.97 9.247807e-06
22 SPBP19A11.02c 244 1:P 218 18.63 -0.50 18.13 1.562869e-06
23 SPBP4G3.02 453 1:N 430 0.89 -17.39 -16.50 1.614081e-02
24 SPBPB7E8.01 569 1:P 540 15.72 -2.07 13.65 1.049254e-05
25 SPBPJ4664.02 3971 1:P 3944 15.03 -7.47 7.56 9.135824e-05
26 SPCC1322.10 262 1:P 237 16.45 -1.78 14.67 6.982318e-06
27 SPCC1742.01 1563 1:P 1539 14.62 -0.35 14.27 8.202445e-06
28 SPCC1795.09 521 1:P 495 14.53 -3.46 11.07 2.773067e-05
29 SPCC24B10.06 156 1:P 129 15.78 -0.95 14.83 6.544299e-06
30 SPCC553.10 349 1:P 323 21.04 -0.36 20.68 4.583121e-07
31 SPCC63.02c 564 1:P 538 15.76 -9.37 6.39 1.312459e-04
32 SPCC757.12 625 1:P 603 20.98 -0.35 20.63 4.697444e-07
33 SPCC970.02 442 1:I 417 -0.23 -31.74 -31.97 8.438813e-02



9 proteins described in the paper have not been predicted as suitable candidates for GPI anchoring:

  1. We could not detect a suitable omega-site for 4 proteins:

    2. SPAC23D3.14c
    SPAPJ760.03c
    SPBC16A3.13
    SPCC970.02

  2. 5 proteins lack an appropriately constructed hydrophobic tail:

    3. SPAC1002.13c
    SPAC1039.11c
    SPAC2E1P3.05c
    SPAC821.09
    SPBP4G3.02



Last modified: 24th September 2003