The Amylase Connection
Step 1 : BLAST-searches with PTG and closely related sequences
**************************************************************
BLAST-search with PTG
/:gpnew|U89924|1888566 protein phosphatase 1 binding prot... 1506 1.2e-154 1
/:trembl|S80360|RS3605_1 product: "hepatic glycogen-bindi... 568 3.0e-55 1
/:trembl|U88313|CEUF15G10_2 gene: "F15G10.1"; Caenorhabd... 211 4.2e-21 2
/:trembl|X78578|HSPPPAGB_1 gene: "PPP1R3"; product: "prot... 217 1.2e-16 1
/:trembl|M65109|OSRG1A_1 gene: "RG1"; product: "protein p... 212 3.9e-16 1
/:swissnew|P40036|GIP2_YEAST GLC7-INTERACTING PROTEIN 2./... 186 1.3e-13 2
/:swissnew|P40187|PIG2_YEAST GSY2 INTERACTING PROTEIN PIG... 172 1.2e-10 1
/:swissnew|P28006|GAC1_YEAST PROTEIN PHOSPHATASE 1 REGULA... 155 2.1e-08 1
/:swiss|P28006|GAC1_YEAST GAC1 PROTEIN.//:trembl|X63941|S... 155 2.1e-08 1
/:swissnew|Q06216|PIG1_YEAST PROTEIN PHOSPHATASE 1 REGULA... 96 0.00013 2
/:swiss|P07683|AMYG_RHIOR GLUCOAMYLASE PRECURSOR (EC 3.2.... 104 0.0085 1
Reciprocal BLAST-searches with the animal (PTG, rGl, cGl, hGl, RG1) and yeast
(GIP2, PIG2, GAC1, PIG1) phosphatases return the same family.
AMYG_RHIOR is a modular protein. The first 150 amino acid have a similarity
to a domain in the phosphatases. Then, the catalytic domain follows in the
sequence.
A reciprocal BLAST-search with the first 200 amino acids of AMYG_RHIOR
(a fungal glycoamylase of family 15) returns phosphatases (including
PTG with P=2.e-8 !! and also GAC1, PIG2, RG1, and GIP2 in the twilight zone)
as well as two other amylases:
/:pironly|JC4510|JC4510 alpha-amylase (EC 3.2.1.1) precur... 126 2.8e-07 1
--> Lipomyces kononenkoae (yeast)
/:swiss|P42042|AMYG_ARXAD GLUCOAMYLASE PRECURSOR (EC 3.2.... 96 4.1e-06 2
--> ARXULA ADENINIVORANS.
EUKARYOTA; FUNGI; DEUTEROMYCOTINA (IMPERFECT FUNGI).
SIMILARITY: BELONGS TO FAMILY 15 OF GLYCOSYL HYDROLASES.
/:gpnew|U89924|1888566 protein phosphatase 1 binding prot... 103 5.2e-05 1
/:swissnew|P28006|GAC1_YEAST PROTEIN PHOSPHATASE 1 REGULA... 76 0.00023 3
/:swiss|P28006|GAC1_YEAST GAC1 PROTEIN.//:trembl|X63941|S... 76 0.00023 3
/:swissnew|P40187|PIG2_YEAST GSY2 INTERACTING PROTEIN PIG... 100 0.00085 1
/:trembl|M65109|OSRG1A_1 gene: "RG1"; product: "protein p... 99 0.0031 1
/:swissnew|P40036|GIP2_YEAST GLC7-INTERACTING PROTEIN 2./... 91 0.024 1
/:trembl|S80360|RS3605_1 product: "hepatic glycogen-bindi... 87 0.033 1
Regions of sequence similarity as given in BLAST alignments in the
BLAST output of the PTG search (sequence positions as in the original
sequences):
PTG 33-293 rGl 31-283
131-237 cGl 173-280
60-234 hGl 61-229
33-241 RG1 36-239
144-233 GIP2 437-533
146-233 PIG2 408-507
149-249 GAC1 258-375
120-199 PIG1 206-275
134-233 AMYG_RHIOR 34-126
I.e., the N-terminus of the domain studied is defined by the sequence of
AMYG_RHIOR. The C-terminus is confined by some short phosphatases (PTG, cGl).
Regions of sequence similarity as given in BLAST alignments in the
BLAST output of the AMYG_RHIOR search (sequence positions as in the original
sequences):
AMYG_RHIOR 35-127 JC4510 49-130
36-129 AMYG_ARXAD 36-131
Step 2 : Extraction of sequence motifs for MOST-searches
********************************************************
We produced a multiple alignment of the 10 sequences (9 phosphatases and
AMYG_RHIOR) with clustalw. Two most significant motifs could be extracted.
motif 1
*******
VFVKNIYFDK
ILVKNIFYDK
VYVKNLSFEK
IFVNNLNFEK
IRVLNISFEK
IRVLNVSFEK
VKVKNVSFEK
VKVQNLAFEK
IKVGNIAFEK
IYVKNIAYSK
results of the MOST-search:
--> only hits with low significance (E>0.242 and R>0.019)
VC21_VACCC
CEF54F7 (KIN, tRNA)
SSD902 (only one of the proteins)
AMYG_ARXAD (was already found --> reciprocal BLAST with AMYG_RHIOR)
MOST output:
....:....|. Score Expected Ratio
>/:swiss|P21105|VC21_ 97: VFmKNISFDK 168, 0.242 0.0192
>/:tremblnew|Z67755|C 300: VFVNNLdFDK 165, 0.334 0.0263
>/:trembl|D90902|SSD9 465: VKVLNIYFgK 161, 0.491 0.0409
>/:swiss|P42042|AMYG_ 50: aFVKNLSYDK 159, 0.579 0.0494
>/:swissnew|P28006|GA ! 3 262: VYVKNLSFEK 207, 0.000366 0.000177
>/:swissnew|P40187|PI ! 1 412: VFVKNIYFDK 200, 0.00169 0.000548
>/:tremblnew|U89924|M ! 6 150: VKVKNVSFEK 198, 0.00241 0.000586
>/:trembl|U88313|CEUF ! 8 194: IKVGNIAFEK 192, 0.00717 0.0014
>/:trembl|S80360|RS36 ! 7 148: VKVQNLAFEK 191, 0.00854 0.00139
>/:swissnew|Q06216|PI ! 4 228: IFVNNLNFEK 188, 0.0143 0.00175
>/:swiss|P07683|AMYG_ ! 9 50: IYVKNIAYSK 188, 0.0143 0.00175
>/:trembl|M65109|OSRG ! 5 148: IRVLNISFEK 183, 0.0329 0.0036
>/:swissnew|P40036|GI ! 2 443: ILVKNIFYDK 177, 0.0788 0.00796
motif 2
*******
EYWDNNDSANY
EYWDNNNGNNY
TYYDNNNGKNY
SFYDNNDYKNY
TFWSNNNGTNY
TFWSNNNGTNY
IFWDNNEGQNY
SYWDSNKGKNY
EYWDSNSGTNY
TYYDNNNSANY
results of the MOST-search:
two new glycohydrolases
1) AMY_CLOAB|P23671 CLOSTRIDIUM ACETOBUTYLICUM
SIMILARITY: BELONGS TO FAMILY 13 OF GLYCOSYL HYDROLASES
MOST output:
....:....|. Score Expected Ratio
>/:swiss|P23671|AMY_C 452: gtWDNNNSNNY 196, 0.019 0.00155
>/:swissnew|P40036|GI ! 2 521: EYWDNNNGNNY 264, 7.43e-07 7.19e-07
>/:swissnew|P28006|GA ! 3 347: TYYDNNNGKNY 256, 3.97e-06 1.28e-06
>/:swissnew|P40187|PI ! 1 495: EYWDNNDSANY 247, 2.17e-05 5.25e-06
>/:trembl|S80360|RS36 ! 7 219: SYWDSNKGKNY 240, 6.9e-05 1.33e-05
>/:trembl|M65109|OSRG ! 5 219: TFWSNNNGTNY 239, 8.17e-05 7.89e-06
>/:gpnew|U89924|18885 ! 6 221: IFWDNNEGQNY 239, 8.17e-05 7.89e-06
>/:trembl|U88313|CEUF ! 8 265: EYWDSNSGTNY 239, 8.17e-05 7.89e-06
>/:swiss|P07683|AMYG_ ! 9 117: TYYDNNNSANY 239, 8.17e-05 7.89e-06
>/:swissnew|Q06216|PI ! 4 318: SFYDNNDYKNY 227, 0.000447 3.93e-05
2) SLAMLC_2|Z86113;
"amlC"; S.lividans amlC gene
MOST output:
....:....|. Score Expected Ratio
>/:tremblnew|Z86113|S 86: GTWDNNgGeNY 202, 0.0101 0.000755
>/:swissnew|P40036|GI ! 2 521: EYWDNNNGNNY 270, 1.68e-07 1.62e-07
>/:swissnew|P28006|GA ! 3 347: TYYDNNNGKNY 257, 4.8e-06 1.55e-06
>/:swissnew|P40187|PI ! 4 495: EYWDNNDSANY 250, 1.81e-05 4.37e-06
>/:swiss|P07683|AMYG_ ! 9 117: TYYDNNNSANY 242, 6.71e-05 1.3e-05
>/:trembl|S80360|RS36 ! 5 219: SYWDSNKGKNY 239, 0.000103 1.67e-05
>/:trembl|M65109|OSRG ! 6 219: TFWSNNNGTNY 238, 0.000119 1.43e-05
>/:gpnew|U89924|18885 ! 7 221: IFWDNNEGQNY 237, 0.000137 1.48e-05
>/:trembl|U88313|CEUF ! 8 265: EYWDSNSGTNY 236, 0.000159 1.53e-05
>/:swiss|P23671|AMY_C ! 1 452: GTWDNNNSNNY 230, 0.000358 3.15e-05
>/:swissnew|Q06216|PI ! 10 318: SFYDNNDYKNY 227, 0.000528 4.26e-05
There are internal repeats in AMY_CLOAB (3 repeats) and in SLAMLC_2 (2 repeats).
Independent BLAST searches were carried out with each repeat.
results of reciprocal BLAST-searches with SLAMLC_2 (first 210 amino acids):
many new amylases
/:trembl|Y00150|BPAMYB_1 product: "precursor polypeptide"... 371 1.9e-33 1
/:swiss|P21543|AMYB_BACPO BETA-AMYLASE (EC 3.2.1.2) / ALP... 368 9.9e-33 1
/:trembl|X53373|UCDSMAMY_1 product: "precursor polypeptid... 305 8.5e-26 1
/:swiss|P06547|AMYB_BACCI BETA-AMYLASE PRECURSOR (EC 3.2.... 184 1.5e-13 1
/:trembl|X55452|BSG6AMA_1 gene: "G6-amylase"; Bacillus s... 181 6.6e-13 1
/:swiss|P23671|AMY_CLOAB PUTATIVE ALPHA-AMYLASE (EC 3.2.1... 137 1.2e-08 1
There are internal repeats in AMYB_BACPO and (less pronounced) also in
UCDSMAMY_1.
Step 3 : Extraction of sequence profiles for SEARCHWISE-searches
****************************************************************
production of a multiple alignment of the amylases (including their repeats)
found in step 2 with clustalw and extraction of a pattern for the alignment
part including motifs 1 and 2
SEARCHWISE-output (BLOSUM62):
1 19470 535 :swiss|P21543|AMYB_BACPO BETA-AMYLASE (EC 3.2.1.2) / ALPHA-AMYLASE (EC
2 19340 648 :trembl|Y00150|BPAMYB_1 product: "precursor polypeptide"; B.polymyxa
3 18560 538 :swiss|P06547|AMYB_BACCI BETA-AMYLASE PRECURSOR (EC 3.2.1.2) (1,4-ALPH
4 18230 1054 :trembl|X53373|UCDSMAMY_1 product: "precursor polypeptide (AA -37 to 1
5 17600 206 :tremblnew|Z86113|SLAMLC_2 gene: "amlC"; S.lividans amlC gene //:gpne
6 17180 940 :trembl|X55452|BSG6AMA_1 gene: "G6-amylase"; Bacillus sp. H-167 G6-am
7 13146 371 :swiss|P23671|AMY_CLOAB PUTATIVE ALPHA-AMYLASE (EC 3.2.1.1) (1,4-ALPHA
--> new findings
8 4748 887 :swiss|P30269|AMY_BUTFI ALPHA-AMYLASE PRECURSOR (EC 3.2.1.1) (1,4-ALPH
9 4660 229 :trembl|S80360|RS3605_1 product: "hepatic glycogen-binding subunit pro
10 4568 427 :trembl|X95759|STSS_1 product: "glycogen (starch) synthase"; S.tubero
11 4568 427 :tremblnew|X94400|STSSSIII_1 gene: "SSSIII"; product: "soluble-starch-
12 4500 278 :trembl|U88313|CEUF15G10_2 gene: "F15G10.1"; Caenorhabditis elegans c
13 4460 231 :tremblnew|U89924|MMU89924_1 product: "protein phosphatase 1 binding p
14 4450 531 :swissnew|P40036|GIP2_YEAST GLC7-INTERACTING PROTEIN 2.//:swiss|P40036
SEARCHWISE-output (GON250):
1 19940 535 :swiss|P21543|AMYB_BACPO BETA-AMYLASE (EC 3.2.1.2) / ALPHA-AMYLASE (EC
2 19840 648 :trembl|Y00150|BPAMYB_1 product: "precursor polypeptide"; B.polymyxa
3 19610 1054 :trembl|X53373|UCDSMAMY_1 product: "precursor polypeptide (AA -37 to 1
4 18980 538 :swiss|P06547|AMYB_BACCI BETA-AMYLASE PRECURSOR (EC 3.2.1.2) (1,4-ALPH
5 18800 208 :tremblnew|Z86113|SLAMLC_2 gene: "amlC"; S.lividans amlC gene //:gpne
6 18510 940 :trembl|X55452|BSG6AMA_1 gene: "G6-amylase"; Bacillus sp. H-167 G6-am
7 14148 466 :swiss|P23671|AMY_CLOAB PUTATIVE ALPHA-AMYLASE (EC 3.2.1.1) (1,4-ALPHA
--> new findings
8 7324 887 :swiss|P30269|AMY_BUTFI ALPHA-AMYLASE PRECURSOR (EC 3.2.1.1) (1,4-ALPH
9 6754 599 :trembl|U22045|BS22045_1 gene: "Amy"; product : "alpha-amylase"; Bacil
--> less likely new findings
10 6251 612 :swiss|P14804|AMYG_NEUCR GLUCOAMYLASE PRECURSOR (EC 3.2.1.3) (GLUCAN 1
11 6044 555 :trembl|D49448|CRGG2A_1 product: "glucoamylase G2"; Corticium rolfsii
12 5601 372 :pdb|1INV|1INV Influenza bLEE40 neuraminidase (sialidase) complexed wi
The starch synthetases STSS_1 and STSSSIII_1 contain internal repeats.
Reciprocal BLAST-searches with the starch synthetases as well as with AMY_BUTFI
do not give new hits in the region of the profile.
Step 4 : BLAST-searches with the sequence of BS22045_1
******************************************************
region of similarity with the profile amyg-2w-gon250
* * * * * * * * * * *
amyg-2w-go 41 GGWTAVPGVAMvSAEDGGWa.KITVPIGsASQLNAAFNNGSGt..WDNNNGNNYN
BS22045_1 542 GNWNTANAIKMNPSSYPTWKATIALPQGKAIEFKFIKKDQAGNVIWESTSNRTYT
amyg-2w-go 93 FS
BS22045_1 597 VP
BLAST-results with BS22045_1 (last part of the sequence)
/:trembl|U22045|BS22045_1 gene: "Amy"; product: "alpha-am... 630 8.1e-62 1
/:pdb|1CYG|1CYG Cyclodextrin glucanotransferase (cgtase) 249 2.1e-21 2
/:swiss|P31797|CDGT_BACST CYCLOMALTODEXTRIN GLUCANOTRANSF... 249 2.5e-21 2
/:swiss|P31746|CDGT_BACS2 CYCLOMALTODEXTRIN GLUCANOTRANSF... 220 2.0e-20 2
/:trembl|D13068|BSCYGT_1 product: "cyclodextrin glucanotr... 220 2.2e-20 2
/:swiss|P22998|AMY_STRVL ALPHA-AMYLASE PRECURSOR (EC 3.2.... 245 3.6e-20 1
/:swiss|P19584|AMYB_THETU BETA-AMYLASE, THERMOPHILIC PREC... 228 1.0e-19 2
/:pdb|1CIU|1CIU cyclodextrin glycosyltransferase(cgtase) 216 1.8e-18 2
/:swiss|P26827|AMY_THETU ALPHA-AMYLASE PRECURSOR (EC 3.2.... 216 2.0e-18 2
/:pdb|1CGT|1CGT Cyclodextrin glycosyltransferase 199 4.9e-16 2
/:pdb|1CGU|1CGU Cyclodextrin glycosyltransferase mutant w... 199 4.9e-16 2
/:swiss|P27036|CDGT_BACOH CYCLOMALTODEXTRIN GLUCANOTRANSF... 208 5.4e-16 1
/:trembl|D90243|BOCGTAA_1 B.ohbensis CGT gene for cyclode... 208 5.4e-16 1
/:swiss|P30920|CDGT_BACCI CYCLOMALTODEXTRIN GLUCANOTRANSF... 199 5.7e-16 2
/:swiss|P14014|CDGT_BACLI CYCLOMALTODEXTRIN GLUCANOTRANSF... 199 5.7e-16 2
/:trembl|A18991|A18991_1 product: "gamma-cyclodextrin gly... 207 6.8e-16 1
/:swiss|P17692|AMYR_BACS8 RAW-STARCH-DIGESTING AMYLASE PR... 199 7.1e-16 2
/:pdb|1CDG|1CDG Cyclodextrin glycosyltransferase (cgtase)... 197 1.0e-15 2
/:pdb|1CGV|1CGV Cyclomaltodextrin glucanotransferase (cgt... 197 1.0e-15 2
/:pdb|1CGW|1CGW Cyclomaltodextrin glucanotransferase (cgt... 197 1.0e-15 2
/:pdb|1CGX|1CGX Cyclomaltodextrin glucanotransferase (cgt... 197 1.0e-15 2
/:pdb|1CGY|1CGY Cyclomaltodextrin glucanotransferase (cgt... 197 1.0e-15 2
/:pdb|1CXF|1CXF cyclodextrin glycosyltransferase(cgtase)M... 197 1.0e-15 2
/:swiss|P43379|CDGU_BACCI CYCLOMALTODEXTRIN GLUCANOTRANSF... 197 1.2e-15 2
/:swiss|P30270|AMY_STRGR ALPHA-AMYLASE PRECURSOR (EC 3.2.... 202 1.6e-15 1
/:swiss|P09794|AMY_STRLM ALPHA-AMYLASE PRECURSOR (EC 3.2.... 202 1.6e-15 1
/:trembl|D49448|CRGG2A_1 product: "glucoamylase G2"; Cor... 202 1.7e-15 1
/:trembl|Z85949|SLAMLB_1 gene: "amlB"; product: "alpha-am... 201 2.1e-15 1
/:swiss|P31835|CDG2_BACMA CYCLOMALTODEXTRIN GLUCANOTRANSF... 202 2.4e-15 1
/:pdb|1PAM|1PAM-A cyclodextrin glucanotransferasebiologic... 201 2.9e-15 1
/:swiss|P09121|CDGT_BACS3 CYCLOMALTODEXTRIN GLUCANOTRANSF... 201 3.1e-15 1
/:swiss|P05618|CDGT_BACS0 CYCLOMALTODEXTRIN GLUCANOTRANSF... 201 3.1e-15 1
/:swiss|P30921|CDGT_BACSP CYCLOMALTODEXTRIN GLUCANOTRANSF... 200 4.0e-15 1
/:swiss|P31747|CDGT_BACSS CYCLOMALTODEXTRIN GLUCANOTRANSF... 191 4.1e-15 2
/:pdb|1KUL|1KUL glucoamylasefragment: binding domain, res... 185 1.2e-14 1
... and many more amylases and related enzymes
Reciprocal BLAST-searches with the related sequence portions do not produce new
hits but verify the family.
Step 5 : The multiple alignment
*******************************
five groups of sequences related to PTG
group G1-ptg.lis
****************
swissnew:pig2_yeast
swissnew:gip2_yeast
swissnew:gac1_yeast
swissnew:pig1_yeast
RG1.gcg
PTG.gcg
hGl.gcg
rGl.gcg
cGl.gcg
swissprot:amyg_rhior
group G2-arxad.lis
******************
swissprot:amyg_arxad
JC4510 (Lip.kon.)
group G3-slam.lis
*****************
amycloab_1
amycloab_2
amycloab_3
slamlc_21
slamlc_22
amyb_bacci.swissprot
amyb_bacp1
amyb_bacp2
bpamyb_1.trembl
bsg6ama_1.trembl
ucdsmamy_1.trembl
group G4-stss.lis
*****************
stss_1
stsssiii_i
(two highly homologous starch synthetase sequences)
group G5-bs22.lis
*****************
bs22045_1
1cgt -- 13
swissprot:AMYB_BACCE -- 14
1kul -- 15
Step 6 : TBLASTN searches in the EST-database ********************************************* PTG /:embl|W28890|HSW28890 53b9 Human retina cDNA rando... +2 732 1.1e-71 1 /:embl|W19091|HS091325 zb14g10.r1 Soares fetal lung... +1 587 6.2e-61 2 /:embl|AA112835|HSAA12835 zn54e03.r1 Stratagene muscle... +2 600 2.5e-57 1 /:embl|R84901|HSR84901 yq27d11.r1 Soares fetal live... +2 458 2.8e-42 1 /:embl|T72137|HS13745 yc63h12.r1 Homo sapiens cDNA... +2 395 1.2e-35 1 /:embl|Z42059|HSC07C021 H. sapiens partial cDNA sequ... +3 341 1.3e-29 1 /:embl|R06045|HS04575 ye89f05.r1 Homo sapiens cDNA... +2 214 1.9e-16 1 /:embl|N88218|HSN2182 K2200F Fetal heart, Lambda Z... +2 201 6.2e-15 1 /:embl|H14696|HS696163 ym19g01.r1 Homo sapiens cDNA... +2 178 1.3e-12 1 /:embl|AA094222|HSAA94222 cl2316.seq.F Fetal heart, La... +2 180 2.2e-12 1 /:embl|AA089749|HSAA89749 chn1002.seq.F Fetal heart, L... +2 164 1.8e-10 1 /:embl|R10183|HS18380 yf35c07.r1 Homo sapiens cDNA... +3 149 6.7e-09 1 --> this is a new human paralogue of PTG /:embl|R04030|CB0301 pk16h02.s1 Caenorhabditis br... +1 81 0.30 1 rGl /:embl|W28890|HSW28890 53b9 Human retina cDNA rando... +2 453 4.0e-42 1 /:embl|W19091|HS091325 zb14g10.r1 Soares fetal lung... +1 337 7.2e-33 2 /:embl|AA112835|HSAA12835 zn54e03.r1 Stratagene muscle... +2 348 1.3e-30 1 /:embl|R84901|HSR84901 yq27d11.r1 Soares fetal live... +2 254 1.2e-20 1 /:embl|Z42059|HSC07C021 H. sapiens partial cDNA sequ... +3 240 6.5e-19 1 /:embl|T72137|HS13745 yc63h12.r1 Homo sapiens cDNA... +2 190 6.3e-14 1 /:embl|R10183|HS18380 yf35c07.r1 Homo sapiens cDNA... +3 141 5.6e-08 1 all in the same region cGl /:embl|R04030|CB0301 pk16h02.s1 Caenorhabditis br... +1 209 1.9e-15 1 /:embl|W28890|HSW28890 53b9 Human retina cDNA rando... +2 167 1.0e-09 1 /:embl|Z42059|HSC07C021 H. sapiens partial cDNA sequ... +3 125 2.9e-06 1 /:embl|AA112835|HSAA12835 zn54e03.r1 Stratagene muscle... +2 106 0.00021 1 hGl /:embl|W28890|HSW28890 53b9 Human retina cDNA rando... +2 191 4.9e-14 1 /:embl|AA112835|HSAA12835 zn54e03.r1 Stratagene muscle... +2 130 2.4e-07 1 /:embl|W19091|HS091325 zb14g10.r1 Soares fetal lung... +1 128 6.7e-07 1 /:embl|Z42059|HSC07C021 H. sapiens partial cDNA sequ... +3 114 3.2e-05 1 /:embl|H21088|HS088173 yn65b01.s1 Homo sapiens cDNA... +1 110 4.8e-05 1 /:embl|H86973|HS973241 yt07f12.s1 Homo sapiens cDNA... +3 106 0.00017 1 /:embl|H20799|HS799168 yn64g01.s1 Homo sapiens cDNA... +3 109 0.00024 1 GIP2 /:embl|W28890|HSW28890 53b9 Human retina cDNA rando... +2 152 1.2e-09 1 /:embl|Z42059|HSC07C021 H. sapiens partial cDNA sequ... +3 124 1.3e-06 1 /:embl|AA112835|HSAA12835 zn54e03.r1 Stratagene muscle... +2 104 9.0e-05 1 /:embl|R10183|HS18380 yf35c07.r1 Homo sapiens cDNA... +3 94 0.0019 1 /:embl|W19091|HS091325 zb14g10.r1 Soares fetal lung... +1 92 0.0062 1
Author: Frank.Eisenhaber@imp.ac.at on internet.