Glycogen- and PP1C-binding motifs of PTG (Protein Targeting to Glycogen) and other protein phosphatases

Peer Bork
Thomas Dandekar
Frank Eisenhaber
Martijn Huynen


The multiple alignment of PTG with phosphatases and glycohydrolases

1. The PP1C-binding region 

Pig2_Yeast      SKSVHFD-----RVLPIKLFNE-NEKPIDVG
Gip2_Yeast      SKSVHFD-----QAAPVKYFAE-DESPINVN
GAC1            EKNVRFA----IELTTVKRFDK-NAEPSSIS
PIG1            GKSVRFA----AHLYTVKKFNT-KLAPISIS
Rg1             GRRVSFADNFGFNLVSVKEFDT-WELPSVST
Hgl             TRRVSFADSFGFNLVSVKEFDC-WELPSAST
Rgl             KKRVSFADNQGLALTMVKVFSE-FDDPLDIP
Ptg             KKRVVFADSKGLSLTAIHVFSD-LPEEPAWD
Cgl             RKSVRFADDCGHELYMVRVATEPSNCPPKLS

2. The glycogen-binding region 

PIG2_YEAST      MVIIGKVFVKNIYFD----KKIIVRYT----WDAWRTFH
GIP2_YEAST      RLIAGRILVKNIFYD----KRVVVRYT----WDSWRTTH
GAC1_YEAST      SKITGLVYVKNLSFE----KYLEIKFT----FNSWRDIH
PIG1_YEAST      CQIWGLIFVNNLNFE----KKIEIKFT----LNNWADIH
RG1             TSMKGIIRVLNISFE----KLVYVRMS----LDDWQTHY
hGl             TSIKGIIRVLNVSFE----KLVYVRMS----LDDWQTHY
PTG             RTVTGTVKVKNVSFE----KKVQVRIT----FDTWKTYT
rGl             KAIAGTVKVQNLAFE----KVVKIRMT----FDTWKSFT
cGl             YKVMGTIKVGNIAFE----KSVFVRYT----MNGWISYM
AMYG_RHIOR      STFSGKIYVKNIAYS----KKVTVIYAD--GSDNWNNNG
amy_cloab2      SGNNFTIYYNGNLAN---SSNVSLHWG----VNGWS---
amy_cloab3      ACKQISVYYNGSLAS--SASNITLHWG----CNGFT---
amy_cloab1      AGDSVTITYDAGTTALKDASNVNLYWG----YDGFS---
bsg6ama_1       QANDITIYYKT-SWT-----NPHIHYSL--NQEAWT---
ucdsmamy_1      EGNLVTIYYKK-GFD-----TPYMHYRP--EGGEWT---
amyb_bacp2      TTNKVTVYYKK-GFN-----SPYIHYRP--AGGSWT---
bpamyb_1        TTNKVTVYYKK-GFN-----SPYIHYRP--AGGSWT---
amyb_bacp1      TGNKVTIYYKK-GFN-----SPYIHYRP--AGGSWT---
amyb_bacci      PVNSVTIYYKK-GFN-----SPYIHYRP--AGGTWT---
slamlc_21       PGNTATVFYYTKTKN---WDRYNLHYAP--DGGSWT---
slamlc_22       DGNRATVYYSTRTLG---WTTANIHYRP--AGGSWT---
1cgt            DQVTVRFVVNNASTT---LGQNLYLTGNVAELGNWSTGS
1kul            TAVAVTFDLT-ATTT---YGENIYLVGSISQLGDWET-S
BS22045_1       KTSNVTFTVNNATTT---SGQNVYVVANIPELGNWNT--
AMYB_BACCE      VPLLSTIVVKNLPTT---IGDTVYITGSRAELGSWTQN-
                      *********
                      motif 1


PIG2_YEAST      ESECVYFSNANGILPGSNMDIFKFSIDDIH---------------NPNDKDSNISQLEFC
GIP2_YEAST      EVECVYISDGDGILPGTNMDIFHFIIDDVS--------------KVDP-----RGKLEFC
GAC1_YEAST      YVTANF--NRTI---NSNVDEFKFTIDLNSLKYILLIKRII---TMEKNTSSCPLNIELC
PIG1_YEAST      YINAHY--NKSV---TPHVDEFKFIIDISALKLNLISKNLIYTNFFERKT-TCLLNLQFC
RG1             DILAEYVPNSCD----GETDQFSFKISLVP--------------PYQKD----GSKVEFC
hGl             DILAEYVPNSCD----GETDQFSFKIVLVP--------------PYQKD----GSKVEFC
PTG             DVDCVYMKNVYS---SSDSDTFSFAIDLPR--------------VIPT-----EEKIEFC
rGl             DFPCQYVKDTYA---GSDRDTFSFDISLPE--------------KIQS-----YERMEFA
cGl             DKAAIYQPST-----SKIQDTFKFDLDLPS--------------SVEK---LQIHQIEFC
AMYG_RHIOR      NTIAASYSAPIS---GSNYEYWTFSASING-------------------------IKEFY
amy_cloab2      --NMQNLAMV-----KDSNGFWEATIAIPA------------------------SSNTLN
amy_cloab3      --SPQDINMV-----KQADGRWLANITLPS------------------------GCYNVN
amy_cloab1      --AATSKAMT-----SLGDNKWQTTITVPK-----------------------EVTKNVN
bsg6ama_1       --TLPGVPLN-----SSEYEGY-AKITIET-----------------------EEGSQLR
ucdsmamy_1      --IVPGIRME-----ESEIAGY-SKLTVDI-----------------------REASKLE
amyb_bacp2      --AAPGVKMQ-----DAEISGY-AKITVDI-----------------------GSASQLE
bpamyb_1        --AAPGVKMQ-----DAEISGY-AKITVDI-----------------------GSASQLE
amyb_bacp1      --AAPGVKMQ-----DAEISGY-AKITVDI-----------------------GSASQLE
amyb_bacci      --DVPGVKMP-----DSEISGY-AKITLDI-----------------------GSASQLE
slamlc_21       --EVPGVAM------EAACTDW-VKRTVPL-----------------------GSAEGLR
slamlc_22       --AVPGVGM------RAACAGW-WKKDVDL-----------------------GAATSLT
1cgt            TAIGPAFNQV-----IHQYPTWYYDVSVPA-----------------------GKQLEFK
1kul            DGIALSADKY-----TSSDPLWYVTVTLPA-----------------------GESFEYK
BS22045_1       ---ANAIKMN-----PSSYPTWKATIALPQ-----------------------GKAIEFK
AMYB_BACCE      ---ISTSLYY-----DSHSNDWRGNVVLPA-----------------------ERNIEFK


PIG2_YEAST      IQYLTWGVDRSRKEYWDNNDSANYKI------DVVTNETRT-
GIP2_YEAST      IHYSTRN-DYEREEYWDNNNGNNYKV------DVVMDGFN--
GAC1_YEAST      CRYDVNN-----ETYYDNNNGKNYHL------FMTTFKKGGE
PIG1_YEAST      CRYDVNGFEY--RSFYDNNDYKNYEI------TISLSAINLN
RG1             IRYETSV-----GTFWSNNNGTNYTL------VCQKKEPEP-
hGl             IRYETSV-----GTFWSNNNGTNYTF------ICQKKEQEP-
PTG             ISYHANG-----RIFWDNNEGQNYRI------VHVQWKPDGV
rGl             VCYECNG-----QSYWDSNKGKNYRI------TRAELRSTQ-
cGl             ICFKANG-----TEYWDSNSGTNYVL------HCEQQQ----
AMYG_RHIOR      IKYEVSG-----KTYYDNNNSANYQV------STSKPTTT--
amy_cloab2      FCFTNG------SS-WDNNNNNNWTL--------NT------
amy_cloab3      MAFKDQ------SGTWDNNNSNNYNF--------SSTNN---
amy_cloab1      FSFTDG------TS-WDNNNGANWNIP----LASNYLPHG--
bsg6ama_1       AAFNNG------RGQWDNNQGRDYVFH----SGINTLADGVI
ucdsmamy_1      VAFNNG------RGAWDSDQENNYLFE----PGVHTYIPSHE
amyb_bacp2      AAFNDG------NNNWDSNNTKNYLFS----TGTSTYTPGSN
bpamyb_1        AAFNDG------NNNWDSNNTKNYLFS----TGTSTYTPGSN
amyb_bacp1      AAFNDG------NNNWDSNNTKNYSFS----TGTSTYTPGNS
amyb_bacci      AAFNDG------NNQWDSNNMRNYFFS----PGTSTYIPGTN
slamlc_21       ATFNNG------SGTWDNNGGENYALG----TGALTVKDGVV
slamlc_22       AAFNNG------NGVWDNNKGADYTLP----AGVTTVADNTV
1cgt            FFKKNGS-----TITWESGSNHTFTTP---ASGTATVTVNWQ
1kul            FIRIESDD----SVEWESDPNREYTVPQACGTSTATVTDTWR
BS22045_1       FIKKDQAG----NVIWESTSNRTYTVP---FSSTGSYTASWN
AMYB_BACCE      AFIKSKDGR---VKSWQTIQQSWNPVP----LKTTSHTSSW-
                               **********
                                motif 2

Last modified: Feb 11 2000