ID   TIAM_MOUSE     STANDARD;      PRT;  1591 AA.
AC   Q60610;
DT   01-NOV-1997 (Rel. 35, Created)
DT   01-NOV-1997 (Rel. 35, Last sequence update)
DT   15-JUL-1999 (Rel. 38, Last annotation update)
DE   T-LYMPHOMA INVASION AND METASTASIS INDUCING PROTEIN 1 (TIAM1 PROTEIN).
GN   TIAM1 OR TIAM-1.
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   SEQUENCE FROM N.A.
RC   STRAIN=BALB/C; TISSUE=BRAIN;
RX   MEDLINE=94243921; PubMed=7999144;
RA   Habets G.G.M., Scholtes E.H.M., Zuydgeest D., van der Kammen R.A.,
RA   Stam J.C., Berns A., Collard J.G.;
RT   "Identification of an invasion-inducing gene, Tiam-1, that encodes a
RT   protein with homology to GDP-GTP exchangers for Rho-like proteins.";
RL   Cell 77:537-549(1994).
CC   -!- FUNCTION: MODULATES THE ACTIVITY OF RHO-LIKE PROTEINS AND CONNECTS
CC       EXTRACELLULAR SIGNALS TO CYTOSKELETAL ACTIVITIES. ACTS AS A GDP-
CC       DISSOCIATION STIMULATOR PROTEIN THAT STIMULATES THE GDP-GTP
CC       EXCHANGE ACTIVITY OF RHO-LIKE GTPASES AND ACTIVATES THEM.
CC       ACTIVATES RAC1, CDC42, AND TO A LESSER EXTENT RHOA (BY
CC       SIMILARITY). AFFECTS INVASIVENESS OF T-LYMPHOMA CELLS.
CC   -!- TISSUE SPECIFICITY: HIGHLY EXPRESSED IN BRAIN AND TESTIS AND AT
CC       LOW OR MODERATE LEVELS IN ALMOST ALL OTHER NORMAL TISSUES. FOUND
CC       IN VIRTUALLY ALL ANALYZED TUMOR CELL LINES INCLUDING B- AND T-
CC       LYMPHOMAS, NEUROBLASTOMAS, MELANOMAS AND CARCINOMAS.
CC   -!- SIMILARITY: CONTAINS 1 DBL-HOMOLOGY DOMAIN (DH).
CC   -!- SIMILARITY: CONTAINS 2 PH DOMAINS.
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DR   EMBL; U05245; AAA18830.1; -.
DR   SWISS-2DPAGE; Q60610; MOUSE.
DR   MGD; MGI:103306; Tiam1.
DR   INTERPRO; IPR000219; -.
DR   INTERPRO; IPR001331; -.
DR   INTERPRO; IPR001478; -.
DR   INTERPRO; IPR001849; -.
DR   PFAM; PF00595; PDZ; 1.
DR   PFAM; PF00169; PH; 2.
DR   PFAM; PF00621; RhoGEF; 1.
DR   PROSITE; PS00741; GDS_CDC24; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
KW   Guanine-nucleotide releasing factor; Myristate.
FT   LIPID         2      2       MYRISTATE (POTENTIAL).
FT   DOMAIN      434    549       PH.
FT   DOMAIN     1047   1239       DH.
FT   DOMAIN     1261   1397       PH.
FT   DOMAIN      595    598       POLY-LYS.
FT   DOMAIN     1445   1449       POLY-ARG.
SQ   SEQUENCE   1591 AA;  177532 MW;  ECC2BAB189A6F019 CRC64;
     MGNAESQNVD HEFYGEKHAS LGRKHTSRSL RLSHKTRRTR HASSGKAIHR NSEVSTRSSS
     TPSIPQSLAE NGLEPFSQEG ALDDFGDPIW VDRVDMGLRP VSYTDSSVTP SVDGSIVLTA
     ASVQSMPDSE ESRLYGDDAT YLAEGGRRQC PYTSNGPTFM ETASFKKKRS KSADIWREDS
     LEFSLSDLSQ EHLTSNEEIL GSAEEKDCEE ARGMETEASP RQLSTCQRAN SLGDLYAQKN
     SGVKANGGPR NRFSSYCRNL VSDIPDLAKH KMPPAAAEET PPYSNYNTLP CRKSHCLSEG
     ATNPQISLSK SMQGRRAKTT QDVNTGEGSE FADSGIEGAT TDTDLLSRRS NATNSSYSPP
     TGRAFVGSDS GSSSTGDRAR QGVYENFRRE LEMSTTNSES LEEAGSAHSD EQSSGTLSSP
     GQSDILLTAA QGTVRKAGAL AVKNFLVHKK NKKVESATRR KWKHYWVSLK GCTLFFYETD
     GRSGIDHNSV PKHAVWVENS IVQAVPEHPK KDFVFCLSNS LGDAFLFQTT SQTELENWIT
     AIHSACAAAV ARHHHKEDTL RLLKSEIKKL EQKIDMDEKM KKMGEMQLSS VTDSKKKKTI
     LDQIFVWEQN LEQFQMDLFR FRCYLASLQG GELPNPKRLL AFASRPTKVA MGRLGIFSVS
     SFHALVAART GEIGVRRRTQ AMSRSASKRR SRFSSLWGLD TTSKKKQGRP TINQVFGEGT
     DAVKRSLEGI FDDTVPDGKR EKEVVLPSVH QHNPDCDIWV HEYFTPSWFC LPNNQPALTV
     VRPGDTARDT LELICKTHQL DHSAHYLRLK FLMENRVQFY IPQPEEDIYE LLYKEIEICP
     KVTQNIHIEK SDAAADNYGF LLSSVDEDGI RRLYVNSVKE TGLASKKGLK AGDEILEINN
     RAAGTLNSSM LKDFLSQPSL GLLVRTYPEP EGGVELLENP PHRVDGPVDL GESPLAFLTS
     NPGHSLSSEQ GSSAETAPEE GEGPDLESSD ETDHSSKSTE QVAAFCRSLH EMSPSDSSPS
     PQDATSPQLA TTRQLSDADK LRKVICELLE TERTYVKDLN CLMERYLKPL QKETFLTQDE
     LDVLFGNLTE MVEFQVEFLK TLEDGVRLVP DLEKLEKVDQ FKKVLFSLGG SFLYYADRFK
     LYSAFCASHT KVPKVLVKAK TDTAFKAFLD AQNPRQQHSS TLESYLIKPI QRVLKYPLLL
     RELFALTDAE SEEHYHLDVA IKTMNKVASH INEMQKIHEE FGAVFDQLIA EQTGEKKEVA
     DLSMGDLLLH TSVIWLNPPA SLGKWKKEPE LAAFVFKTAV VLVYKDGSKQ KKKLVGSHRL
     SIYEEWDPFR FRHMIPTEAL QVRALPSADA EANAVCEIVH VKSESEGRPE RVFHLCCSSP
     ESRKDFLKSV HSILRDKHRR QLLKTESLPS AQQYVPFGGK RLCALKGARP AMSRAVSAPS
     KSLGRRRRRL ARNRFTIDSD AISASSPEKE PQQPAGGGDT DRWVEEQFDL AQYEEQDDIK
     ETDILSDDDE FCESLKGASV DRDLQEQLQA ASISQRARGR RTLDSHASRM TQLKKQAALS
     GINGGLESAS EEVIWVRRED FAPSRKLNTE I
//