ID   POLG_POL3L     STANDARD;      PRT;  2206 AA.
AC   P03302; Q84783; Q84784; Q84785; Q84786; Q84787; Q84788; Q84789;
AC   Q84790; Q98592; Q98593; Q98594;
DT   21-JUL-1986 (Rel. 01, Created)
DT   21-JUL-1986 (Rel. 01, Last sequence update)
DT   30-MAY-2000 (Rel. 39, Last annotation update)
DE   GENOME POLYPROTEIN [CONTAINS: COAT PROTEINS VP1 TO VP4; CORE PROTEINS
DE   P2A TO P2C, P3A; GENOME-LINKED PROTEIN VPG; PICORNAIN 3C
DE   (EC 3.4.22.28) (PROTEASE 3C) (P3C); RNA-DIRECTED RNA POLYMERASE P3D
DE   (EC 2.7.7.48)].
OS   Poliovirus type 3 (strains P3/Leon/37 and P3/Leon 12A[1]B).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Picornaviridae;
OC   Enterovirus.
OX   NCBI_TaxID=12088;
RN   [1]
RP   SEQUENCE FROM N.A.
RC   STRAIN=P3/LEON/37;
RX   MEDLINE=84170338; PubMed=6324200;
RA   Stanway G., Hughes P.J., Mountford R.C., Reeve P., Minor P.D.,
RA   Schild G.C., Almond J.W.;
RT   "Comparison of the complete nucleotide sequences of the genomes of the
RT   neurovirulent poliovirus P3/Leon/37 and its attenuated Sabin vaccine
RT   derivative P3/Leon 12a1b.";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:1539-1543(1984).
RN   [2]
RP   SEQUENCE FROM N.A.
RC   STRAIN=P3/LEON 12A[1]B;
RX   MEDLINE=83299239; PubMed=6310508;
RA   Stanway G., Cann A.J., Hauptmann R., Hughes P., Clarke L.D.,
RA   Mountford R.C., Minor P.D., Schild G.C., Almond J.W.;
RT   "The nucleotide sequence of poliovirus type 3 leon 12 a1b: comparison
RT   with poliovirus type 1.";
RL   Nucleic Acids Res. 11:5629-5643(1983).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 1-878.
RX   MEDLINE=95120467; PubMed=7820548;
RA   Grant R.A., Hiremath C.N., Filman D.J., Syed R., Andries K.,
RA   Hogle J.M.;
RT   "Structures of poliovirus complexes with anti-viral drugs:
RT   implications for viral stability and drug design.";
RL   Curr. Biol. 4:784-797(1994).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 1-878.
RA   Syed R., Filman D.J., Hogle J.M.;
RL   Submitted (MAR-1995) to the PDB data bank.
CC   -!- FUNCTION: P3C POLYPEPTIDE IS A PROTEASE THAT CLEAVES AT CERTAIN
CC       Q/G SITES IN THE POLYPROTEIN. IT MAY BE A CYSTEINE PROTEASE.
CC   -!- SUBUNIT: THE VIRUS CAPSID IS COMPOSED OF 60 ICOSAHEDRAL UNITS,
CC       EACH OF WHICH IS COMPOSED OF ONE COPY EACH OF PROTEINS VP1, VP2,
CC       VP3, AND VP4.
CC   -!- PTM: SPECIFIC ENZYMATIC CLEAVAGES IN VIVO YIELD MATURE PROTEINS.
CC   -!- MISCELLANEOUS: THE SEQUENCE OF STRAIN SABIN VACCINE P3/LEON/37 IS
CC       SHOWN.
CC   -!- MISCELLANEOUS: THE STRAIN SABIN VACCINE P3/LEON/37 IS THE
CC       PROGENITOR OF THE STRAIN SABIN VACCINE P3/LEON 12A[1]B.
CC   -!- SIMILARITY: THE PROTEASE BELONGS TO PEPTIDASE FAMILY C3.
CC   --------------------------------------------------------------------------
CC   This SWISS-PROT entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use  by  non-profit  institutions as long  as its content  is  in  no  way
CC   modified and this statement is not removed.  Usage  by  and for commercial
CC   entities requires a license agreement (See http://www.isb-sib.ch/announce/
CC   or send an email to license@isb-sib.ch).
CC   --------------------------------------------------------------------------
DR   EMBL; K01392; AAA46914.1; -.
DR   EMBL; X00925; CAA25444.1; -.
DR   PIR; A03900; GNNY4P.
DR   PDB; 1PIV; 20-JUL-95.
DR   PDB; 1PVC; 15-SEP-95.
DR   PDB; 1VBA; 11-JUL-96.
DR   PDB; 1VBB; 11-JUL-96.
DR   PDB; 1VBC; 11-JUL-96.
DR   PDB; 1VBE; 11-JUL-96.
DR   MEROPS; C03.001; -.
DR   MEROPS; C03.020; -.
DR   INTERPRO; IPR000081; -.
DR   INTERPRO; IPR000199; -.
DR   INTERPRO; IPR000605; -.
DR   INTERPRO; IPR001205; -.
DR   INTERPRO; IPR001676; -.
DR   INTERPRO; IPR002527; -.
DR   PFAM; PF00548; Cys-protease-3C; 1.
DR   PFAM; PF00947; Pico_P2A; 1.
DR   PFAM; PF01552; Pico_P2B; 1.
DR   PFAM; PF00680; RNA_dep_RNA_pol; 1.
DR   PFAM; PF00910; RNA_helicase; 1.
DR   PFAM; PF00073; rhv; 3.
KW   Polyprotein; Coat protein; Core protein; Transferase;
KW   RNA-directed RNA polymerase; Hydrolase; Thiol protease; Myristate;
KW   3D-structure.
FT   CHAIN         2     69       COAT PROTEIN VP4.
FT   CHAIN        70    340       COAT PROTEIN VP2.
FT   CHAIN       341    578       COAT PROTEIN VP3.
FT   CHAIN       579    878       COAT PROTEIN VP1.
FT   CHAIN       879   1027       CORE PROTEIN P2A.
FT   CHAIN      1028   1124       CORE PROTEIN P2B.
FT   CHAIN      1125   1453       CORE PROTEIN P2C.
FT   CHAIN      1454   1540       CORE PROTEIN P3A.
FT   CHAIN      1541   1562       GENOME-LINKED PROTEIN VPG.
FT   CHAIN      1563   1745       PICORNAIN 3C.
FT   CHAIN      1746   2206       RNA-DIRECTED RNA POLYMERASE P3D.
FT   LIPID         2      2       MYRISTATE.
FT   ACT_SITE   1709   1709       PROTEASE (POTENTIAL).
FT   ACT_SITE   1723   1723       PROTEASE (POTENTIAL).
FT   VARIANT     431    431       S -> F (IN P3/LEON 12A[1]B).
FT   VARIANT     864    864       K -> R (IN P3/LEON 12A[1]B).
FT   VARIANT     908    908       T -> A (IN P3/LEON 12A[1]B).
SQ   SEQUENCE   2206 AA;  246163 MW;  4766B15C861F66D3 CRC64;
     MGAQVSSQKV GAHENSNRAY GGSTINYTTI NYYKDSASNA ASKQDYSQDP SKFTEPLKDV
     LIKTAPALNS PNVEACGYSD RVLQLTLGNS TITTQEAANS VVAYGRWPEF IRDDEANPVD
     QPTEPDVATC RFYTLDTVMW GKESKGWWWK LPDALRDMGL FGQNMYYHYL GRSGYTVHVQ
     CNASKFHQGA LGVFAIPEYC LAGDSDKQRY TSYANANPGE RGGKFYSQFN KDNAVTSPKR
     EFCPVDYLLG CGVLLGNAFV YPHQIINLRT NNSATIVLPY VNALAIDSMV KHNNWGIAIL
     PLSPLDFAQD SSVEIPITVT IAPMCSEFNG LRNVTAPKFQ GLPVLNTPGS NQYLTSDNHQ
     SPCAIPEFDV TPPIDIPGEV KNMMELAEID TMIPLNLEST KRNTMDMYRV TLSDSADLSQ
     PILCLSLSPA SDPRLSHTML GEVLNYYTHW AGSLKFTFLF CGSMMATGKI LVAYAPPGAQ
     PPTSRKEAML GTHVIWDLGL QSSCTMVVPW ISNVTYRQTT QDSFTEGGYI SMFYQTRIVV
     PLSTPKSMSM LGFVSACNDF SVRLLRDTTH ISQSALPQGI EDLISEVAQG ALTLSLPKQQ
     DSLPDTKASG PAHSKEVPAL TAVETGATNP LAPSDTVQTR HVVQRRSRSE STIESFFARG
     ACVAIIEVDN EQPTTRAQKL FAMWRITYKD TVQLRRKLEF FTYSRFDMEF TFVVTANFTN
     ANNGHALNQV YQIMYIPPGA PTPKSWDDYT WQTSSNPSIF YTYGAAPARI SVPYVGLANA
     YSHFYDGFAK VPLKTDANDQ IGDSLYSAMT VDDFGVLAVR VVNDHNPTKV TSKVRIYMKP
     KHVRVWCPRP PRAVPYYGPG VDYKNNLDPL SEKGLTTYGF GHQNKAVYTA GYKICNYHLA
     TKEDLQNTVS IMWNRDLLVV ESKAQGTDSI ARCNCNAGVY YCESRRKYYP VSFVGPTFQY
     MEANDYYPAR YQSHMLIGHG FASPGDCGGI LRCQHGVIGI VTAGGEGLVA FSDIRDLYAY
     EEEAMEQGIS NYIESLGAAF GSGFTQQIGD KISELTSMVT STITEKLLKN LIKIISSLVI
     ITRNYEDTTT VLATLALLGC DVSPWQWLKK KACDTLEIPY VIRQGDSWLK KFTEACNAAK
     GLEWVSNKIS KFIDWLRERI IPQARDKLEF VTKLKQLEML ENQISTIHQS CPSQEHQEIL
     FNNVRWLSIQ SKRFAPLYAL EAKRIQKLEH TINNYIQFKS KHRIEPVCLL VHGSPGTGKS
     VATNLIARAI AEKENTSTYS LPPDPSHFDG YKQQGVVIMD DLNQNPDGAD MKLFCQMVST
     VEFIPPMASL EEKGILFTSN YVLASTNSSR ITPPTVAHSD ALARRFAFDM DIQVMGEYSR
     DGKLNMAMAT ETCKDCHQPA NFKRCCPLVC GKAIQLMDKS SRVRYSVDQI TTMIINERNR
     RSNIGNCMEA LFQGPLQYKD LKIDIKTRPP PECINDLLQA VDSQEVRDYC EKKGWIVNIT
     SQVQTERNIN RAMTILQAVT TFAAVAGVVY VMYKLFAGHQ GAYTGLPNKR PNVPTIRAAK
     VQGPGFDYAV AMAKRNIVTA TTSKGEFTML GVHDNVAILP THASPGESIV IDGKEVEILD
     AKALEDQAGT NLEITIITLK RNEKFRDIRQ HIPTQITETN DGVLIVNTSK YPNMYVPVGA
     VTEQGYLNLG GRQTARILMY NFPTRAGQCG GVITCTGKVI GMHVGGNGSH GFAAALKRSY
     FTQSQGEIQW MRPSKEAGYP IINAPTKTKL EPSAFHYVFE GVKEPAVLTK NDPRLKTDFE
     EAIFSKYVGN KITEVDEYMK EAVDHYAGQL MSLDISTEQM CLEDAMYGTD GLEALDLSTS
     AGYPYVAMGK KKRDILNKQT RDTKEMQRLL DAYGINLPLV TYVKDELRSK TKVEQGKSRL
     IEASSLNDSV AMRMAFGNLY AAFHRNPGVV TGSAVGCDPD LFWSKIPVLM EEKLFAFDYT
     GYDASLSPAW FEALKMVLEK IGFGDRVDYI DYLNHSHHLY KNKIYCVKGG MPSGCSGTSI
     FNSMINNLII RTLLLKTYKG IDLDHLKMIA YGDDVIASYP HEVDASLLAQ SGKDYGLTMT
     PADKSATFET VTWENVTFLK RFFRADEKYP FLIHPVMPMK EIHESIRWTK DPRNTQDHVR
     SLCLLAWHNG EEEYNKFLAK IRSVPIGRAL LLPEYSTLYR RWLDSF
//