ID   POLG_POL2W     STANDARD;      PRT;  2205 AA.
AC   P23069;
DT   01-NOV-1991 (Rel. 20, Created)
DT   01-NOV-1991 (Rel. 20, Last sequence update)
DT   15-DEC-1998 (Rel. 37, Last annotation update)
DE   GENOME POLYPROTEIN [CONTAINS: COAT PROTEINS VP1 TO VP4; CORE PROTEINS
DE   P2A TO P2C, P3A; GENOME-LINKED PROTEIN VPG; PICORNAIN 3C
DE   (EC 3.4.22.28) (PROTEASE 3C) (P3C); RNA-DIRECTED RNA POLYMERASE P3D
DE   (EC 2.7.7.48)].
OS   Poliovirus type 2 (strain W-2).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Picornaviridae;
OC   Enterovirus.
OX   NCBI_TaxID=12085;
RN   [1]
RP   SEQUENCE FROM N.A.
RX   MEDLINE=90155230; PubMed=2154539;
RA   Pevear D.C., Oh C.K., Cunningham L.L., Calenoff M., Jubelt B.;
RT   "Localization of genomic regions specific for the attenuated, mouse-
RT   adapted poliovirus type 2 strain W-2.";
RL   J. Gen. Virol. 71:43-52(1990).
CC   -!- FUNCTION: P3C POLYPEPTIDE IS A PROTEASE THAT CLEAVES AT CERTAIN
CC       Q/G SITES IN THE POLYPROTEIN. IT MAY BE A CYSTEINE PROTEASE.
CC   -!- SUBUNIT: THE VIRUS CAPSID IS COMPOSED OF 60 ICOSAHEDRAL UNITS,
CC       EACH OF WHICH IS COMPOSED OF ONE COPY EACH OF PROTEINS VP1, VP2,
CC       VP3, AND VP4.
CC   -!- PTM: SPECIFIC ENZYMATIC CLEAVAGES IN VIVO YIELD MATURE PROTEINS.
CC   -!- SIMILARITY: THE PROTEASE BELONGS TO PEPTIDASE FAMILY C3.
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DR   EMBL; D00625; BAA00516.1; ALT_SEQ.
DR   PIR; A34032; GNNY2W.
DR   HSSP; P03299; 1POV.
DR   MEROPS; C03.001; -.
DR   MEROPS; C03.020; -.
DR   INTERPRO; IPR000081; -.
DR   INTERPRO; IPR000199; -.
DR   INTERPRO; IPR000605; -.
DR   INTERPRO; IPR001205; -.
DR   INTERPRO; IPR001676; -.
DR   INTERPRO; IPR002527; -.
DR   PFAM; PF00548; Cys-protease-3C; 1.
DR   PFAM; PF00947; Pico_P2A; 1.
DR   PFAM; PF01552; Pico_P2B; 1.
DR   PFAM; PF00680; RNA_dep_RNA_pol; 1.
DR   PFAM; PF00910; RNA_helicase; 1.
DR   PFAM; PF00073; rhv; 3.
KW   Polyprotein; Coat protein; Core protein; Transferase;
KW   RNA-directed RNA polymerase; Hydrolase; Thiol protease; Myristate.
FT   CHAIN         2     69       COAT PROTEIN VP4 (P1A).
FT   CHAIN        70    340       COAT PROTEIN VP2 (P1B).
FT   CHAIN       341    578       COAT PROTEIN VP3 (P1C).
FT   CHAIN       579    879       COAT PROTEIN VP1 (P1D).
FT   CHAIN       880   1028       PROTEASE 2A.
FT   CHAIN      1029   1125       CORE PROTEIN 2B.
FT   CHAIN      1126   1454       CORE PROTEIN 2C.
FT   CHAIN      1455   1541       CORE PROTEIN 3A.
FT   CHAIN      1542   1563       GENOME-LINKED PROTEIN VPG.
FT   CHAIN      1564   1746       PICORNAIN 3C.
FT   CHAIN      1747   2205       RNA-DIRECTED RNA POLYMERASE 3D.
FT   LIPID         2      2       MYRISTATE (BY SIMILARITY).
FT   ACT_SITE   1710   1710       PROTEASE (POTENTIAL).
FT   ACT_SITE   1724   1724       PROTEASE (POTENTIAL).
SQ   SEQUENCE   2205 AA;  245701 MW;  2A42AB039E0254AD CRC64;
     MGAQVSSQKV GAHENSNRAY GGSTINYTTI NYYRDSASNA ASKQDFAQDP SKFTEPIKDV
     LIKTAPTLNS PNIEACGYSD RVMQLTLGNS TITTQEAANS VVAYGRWPEY IKDSEANPVD
     QPTEPDVAAC RFYTLDTVTW RKESRGWWWK LPDALKDMGL FGQNMFYHYL GRASYTVHVQ
     CNASKFHQGA LGVFAVPEMC LAGDSATHML TKYENANPGE KGGEFKGSFT LDTNATNPAR
     NFCPVDYLFG SGVLAGNAFV YPHQIINLRT NNCATLVLPY VNSLSIDSMT KHNNWGIAIL
     PLAPLDFATE SSTEIPITLT IAPMCCEFNG LRNITVPRTQ GLPVLNTPGS NQYLTADNYQ
     SPCAIPEFDV TPPIDIPGEV RNMMELAEID TMIPLNLTSQ RKNTMDMYRV ELNDAAHSDT
     PILCLSLSPA SDPRLAHTML GEILNYYTHW AGSLKFTFLF CGSMMATGKL LVSYAPPGAK
     APESRKEAML GTHVIWDIGL QSSCTMVVPW ISNTTYRQTI NDSFTEGGYI SMFYQTRVVV
     PLSTPRKMDI LGFVSACNDF SVRLLRDTTH ISQEVMPQGL GDLIEGVVEG VTRNALTPLT
     PVNNLPDTRS SGPAHSKETP ALTAVETGAT NPLVPSDTVQ TRHVIQKRTR SESTVESFFA
     RGACVAIIEV DNDAPTRRAS KLFSVWKITY KDTVQLRRKL EFFTYSRFDM EFTFVVTSNY
     TDANNGHALN QVYQIMYIPP GAPIPGKRND YTWQTSSNPS VFYTYGAPPA RISVPYVGIA
     NAYSHFYDGF AKVPLAGQAS TEGDSLYGAA SLNDFGSLAV RVVNDHNPTK LTSKIRVYMK
     PKHVRVWCPR PPRAVPYYGP GVDYKDGLTP LPEKGLITYG FGHQNKAVYT AGYKICNYHL
     ATQEDLQNAI NIMWIRDLLV VESKAQGIDS IARCNCHTGV YYCESRRKYY PVSFVGPTFQ
     YMEANEYYPA RYQSHMLIGH GFASPGDCGG ILRCQHGVIG IITAGGEGLV AFSDIRDLYA
     YEVEAMEQGV SNYIESLGAA FGSGFTQQIG NKISELTSMV TSTITEKLLK NLIKIISSLV
     IITRNYEDTT TVLATLALLG CDASPWQWLK KKACDILEIP YIMRQGDSWL KKFTEACNAA
     KGLEWVSNKI SKFIDWLKEK IIPQARDKLE FVTKLKQLEM LENQIATIHQ SCPSQEHQEI
     LFNNVRWLSI QSRRFAPLYA VEAKRIQKLE HTINNYVQFK SKHRIEPVCL LVHGSPGTGK
     SVATNLIARA IAEKENTSTY SLPPDPSHFD GYKQQGVVIM DDLNQNPDGA DMKLFCQMVS
     TVEFIPPMAS LEEKGILFTS NYVLASTNSS RITPPTVAHS DALARRFAFD MDIQIMSEYS
     RDGKLNMAMA TEMCKNCHQP ANFKRCCPLV CGKAIQLMDK SSRVRYSIDQ ITTMIINERN
     RRSSIGNCME ALFQSPLQYK DLKIDIKTTP PPECINDLLH AVDSQEVRDY CEKKGWIADI
     TSQVQTERNI NRAMTILQAV TTFAAVAGVV YVMYKLFAGH QGAYTGLPNK RPNVPTIRTA
     KVQGPGFDYA VAMAKRNILT ATTIKGEFTM LGVHDNVAIL PTHASPGETI VIDGKEVEVL
     DAKALEDQAG TNLEITIVTL KRNEKFRDIR PHIPTQITET NDGVLIVNTS KYPNMYVPVG
     AVTEQGYLNL GGRQTARTLM YNFPTRAGQC GGVITCTGKV IGMHVGGNGS HGFAAALKRS
     YFTQSQGEIQ WMRPSKEVGY PVINAPSKTK LEPSAFHYVF EGVKEPAVLT KSDPRLKTDF
     EEAIFSKYVG NKITEVDEYM KEAVDHYAGQ LMSLDINTEQ MCLEDAMYGT DGLEALDLST
     SAGYPYVAMG KKKRDILNKQ TRDTKEMQRL LDTYGINLPL VTYVKDELRS KTKVEQGKSR
     LIEASSLNDS VAMRMAFGNL YAAFHKNPGV VTGSAVGCDP DLFWSKIPVL MEEKLFDYTG
     YDASLSPAWF EALKMVLEKI GFGDRVDYID YLNHSHHLYK NKTYCVKGGM PSGCSGTSIF
     NSMINNLIIR TLLLKTYKGI DLDHLKMIAY GDDVIASYPH EVDASLLAQS GKDYGLTMTP
     ADKSATFETV TWENVTFLKR FFRADEKYPF LVHPVMPMKE IHESIRWTKD PRNTQDHVRS
     LCLLAWHSGE EEYNKFLAKI RSVPIGRALL LPEYSTLYRR WLDSF
//