ID   POLG_HE701     STANDARD;      PRT;  2194 AA.
AC   P32537;
DT   01-OCT-1993 (Rel. 27, Created)
DT   01-NOV-1995 (Rel. 32, Last sequence update)
DT   15-DEC-1998 (Rel. 37, Last annotation update)
DE   GENOME POLYPROTEIN [CONTAINS: COAT PROTEINS VP1 TO VP4; CORE PROTEINS
DE   P2A TO P2C, P3A; GENOME-LINKED PROTEIN VPG; PICORNAIN 3C
DE   (EC 3.4.22.28) (PROTEASE 3C) (P3C); RNA-DIRECTED RNA POLYMERASE P3D
DE   (EC 2.7.7.48)].
OS   Human enterovirus 70 (strain J670/71) (EV 70).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Picornaviridae;
OC   Enterovirus.
OX   NCBI_TaxID=31915;
RN   [1]
RP   SEQUENCE FROM N.A.
RX   MEDLINE=91037960; PubMed=2172447;
RA   Ryan M.D., Jenkins O., Hughes P.J., Brown A., Knowles N.J., Booth D.,
RA   Minor P.D., Almond J.W.;
RT   "The complete nucleotide sequence of enterovirus type 70:
RT   relationships with other members of the picornaviridae.";
RL   J. Gen. Virol. 71:2291-2299(1990).
CC   -!- FUNCTION: P3C POLYPEPTIDE IS A PROTEASE THAT CLEAVES AT CERTAIN
CC       Q/G SITES IN THE POLYPROTEIN. IT MAY BE A CYSTEINE PROTEASE.
CC   -!- SUBUNIT: THE VIRUS CAPSID IS COMPOSED OF 60 ICOSAHEDRAL UNITS,
CC       EACH OF WHICH IS COMPOSED OF ONE COPY EACH OF PROTEINS VP1, VP2,
CC       VP3, AND VP4.
CC   -!- PTM: SPECIFIC ENZYMATIC CLEAVAGES IN VIVO YIELD MATURE PROTEINS.
CC   -!- SIMILARITY: THE PROTEASE BELONGS TO PEPTIDASE FAMILY C3.
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DR   EMBL; D00820; BAA18891.1; -.
DR   PIR; A36253; GNNYE7.
DR   HSSP; P03299; 1POV.
DR   MEROPS; C03.001; -.
DR   MEROPS; C03.020; -.
DR   INTERPRO; IPR000081; -.
DR   INTERPRO; IPR000199; -.
DR   INTERPRO; IPR000605; -.
DR   INTERPRO; IPR001205; -.
DR   INTERPRO; IPR001676; -.
DR   INTERPRO; IPR002527; -.
DR   PFAM; PF00548; Cys-protease-3C; 1.
DR   PFAM; PF00947; Pico_P2A; 1.
DR   PFAM; PF01552; Pico_P2B; 1.
DR   PFAM; PF00680; RNA_dep_RNA_pol; 1.
DR   PFAM; PF00910; RNA_helicase; 1.
DR   PFAM; PF00073; rhv; 3.
KW   Polyprotein; Coat protein; Core protein; Transferase;
KW   RNA-directed RNA polymerase; Hydrolase; Thiol protease; Myristate.
FT   CHAIN         2     69       COAT PROTEIN VP4 (P1A).
FT   CHAIN        70    319       COAT PROTEIN VP2 (P1B).
FT   CHAIN       320    561       COAT PROTEIN VP3 (P1C).
FT   CHAIN       562    871       COAT PROTEIN VP1 (P1D).
FT   CHAIN       872   1014       CORE PROTEIN P2A.
FT   CHAIN      1015   1113       CORE PROTEIN P2B.
FT   CHAIN      1114   1443       CORE PROTEIN P2C.
FT   CHAIN      1444   1532       CORE PROTEIN P3A.
FT   CHAIN      1533   1554       GENOME-LINKED PROTEIN VPG (P3B).
FT   CHAIN      1555   1737       PICORNAIN 3C.
FT   CHAIN      1738   2194       RNA-DIRECTED RNA POLYMERASE P3D.
FT   LIPID         2      2       MYRISTATE (BY SIMILARITY).
FT   ACT_SITE   1701   1701       PROTEASE (POTENTIAL).
FT   ACT_SITE   1715   1715       PROTEASE (POTENTIAL).
SQ   SEQUENCE   2194 AA;  244590 MW;  15DBAE96EE06673C CRC64;
     MGAQVSRQQT GTHENANVAT GGSSITYNQI NFYKDSYAAS ASKQDFSQDP SKFTEPVAEA
     LKAGAPVLKS PSAEACGYSD RVLQLKLGNS SIVTQEAANI CCAYGEWPTY LPDNEAVAID
     KPTQPETSTD RFYTLKSKKW ESNSTGWWWK LPDALNQIGM FGQNVQYHYL YRSGFLCHVQ
     CNATKFHQGT LLIVAIPEHQ IGKKGTGTSA SFAEVMKGAE GGVFEQPYLL DDGTSLACAL
     VYPHQWINLR TNNSATIVLP WMNSAPMDFA LRHNNWTLAV IPVCPLAGGT GNTNTYVPIT
     ISIAPMCAEY NGLRNAITQG VPTCLLPGSN QFLTTDDHSS APAFPDFSPT PEMHIPGQVH
     SMLEIVQIES MMEINNVNDA SGVERLRVQI SAQSDMDQLL FNIPLDIQLE GPLRNTLLGN
     ISRYYTHWSG SLEMTFMFCG SFMTTGKLII CYTPPGGSSP TDRMQAMLAT HVVWDFGLQS
     SITIIIPWIS GSHYRMFNTD AKAINANVGY VTCFMQTNLV APVGAADQCY IVGMVAAKKD
     FNLRLMRDSP DIGQSAILPE QAATTQIGEI VKTVANTVES EIKAELGVIP SLNAVETGAT
     SNTEPEEAIQ TRTVINMHGT AECLVENFLG RSALVCMRSF EYKNHSTSTS SIQKNFFIWT
     LNTRELVQIR RKMELFTYLR FDTEITIVPT LRLFSSSNVS FSGLPNLTLQ AMYVPTGARK
     PSSQDSFEWQ SACNPSVFFK INDPPARLTI PFMSINSAYA NFYDGFAGFE KKATVLYGIN
     PANTMGNLCL RVVNSYQPVQ YTLTVRVYMK PKHIKAWAPR APRTMPYTNI LNNNYAGRSA
     APNAPTAIVS HRSTIKTMPN DINLTTAGPG YGGAFVGSYK IINYHLATDE EKERSVYVDW
     QSDVLVTTVA AHGKHQIARC RCNTGVYYCK HKNRSYPVCF EGPGIQWINE SDYYPARYQT
     NTLLAMGPCQ PGDCGGLLVC SHGVIGLVTA GGEGIVAFTD IRNLLWLEDD AMEQGITDYI
     QNLGSAFGTG FTETISEKAK EIQNMLVGED SLLEKLLKAL IKIVSAMVIV IRNSEDLVTV
     TATLALLGCN DSPWAFLKQK VCSYLGIPYT IRQSDSWLKK FTEACNALRG LDWLAQKIDK
     FINWLKTKIL PEAREKHEFV QKLKQLPVIE SQINTIEHSC PNSEXQQALF NNVQYYSHYC
     KKYAPLYALE AKRVSALERK INNYIQFKSK SRIEPVCLII HGSPGTGKSV ASNLIARAIT
     EKLGGDSYSL PPDPKYFDGY KQQTVVLMDD LMQNPDGNDI AMFCQMVSTV DFIPPMASLE
     EKGTLYTSPF LIATTNAGSI HAPTVSDSKA LARRFKFDME IESMESYKDG VRLDMFKAVE
     LCNPEKCRPT NYKKCCPLIC GKAIQFRDKR TNVRYSVDML VTEMIKEYRI RNSTQDKLEA
     LFQGPPTFKE IKISVTPETP APDAINDLLR SIDSQEVRDY CQKKGWIVMH PPTELVVDKH
     ISRAFIALQA ITTFVSIAGV VYVIYKLFAG IQGPYTGLPN QKPKVPTLRT AKVQGPSLDF
     AQAIMRKNTV IARTSKGEFT MLGIYDRIAV VPTHASVEEE IYINDVPVKV KDAYALRDIN
     DVNLEITVVE LDRNEKFRDI RGFLPKYEDD YNDAILSVNT SKFPNMYIPV GQTLNYGFLN
     LGGTPTHRIL MYNFPTRAGQ CGGVVTTTGK VIGIHVGGNG AQGFAAMLLQ NYFTEKQGEI
     VSIEKTGVFI NAPAKTKLEP SVFHEVFEGV KEPAVLHSKD KRLKVDFEEA IFSKYVGNKT
     MLMDEYMEEA VDHYVGCLEP LDISTEPIKL EEAMYGMDGL EALDLTTSAG YPYLLQGKKK
     RDIFNRQTRD TTEMTKMLDK YGVDLPFVTF VKDELRSREK VEKGKSRLIE ASSLNDSVAM
     RVAFGNLYAT FHKNPGVATG SAVGCDPDLF WSKIPVXLDG KIFAFDYTGY DASLSPVWFA
     CLKKTLVKLG YTHQTAFVDY LCHSVHLYKD RKYIVNGGMP SGSSGTSIFN TMINNIIIRT
     LLLKVYKGID LDQFKMIAYG DDVIASYPHE IDPGLLAKAG KEYGLIMTPA DKSSGFTETT
     WENVTFLKRY FRADEQYPFL IHPVMPMKEI HESIRWTKDP RNTQDHVRSL CLLAWHNGEE
     TYNEFCRKIR TVPVGRALAL PVYSSLRRKW LDSF
//