ID   POLG_EMCVD     STANDARD;      PRT;  2292 AA.
AC   P17594;
DT   01-AUG-1990 (Rel. 15, Created)
DT   01-FEB-1996 (Rel. 33, Last sequence update)
DT   15-DEC-1998 (Rel. 37, Last annotation update)
DE   GENOME POLYPROTEIN [CONTAINS: COAT PROTEINS VP1 TO VP4; CORE PROTEINS
DE   P2A TO P2C, P3A; GENOME-LINKED PROTEIN VPG; PICORNAIN 3C
DE   (EC 3.4.22.28) (PROTEASE 3C) (P3C); RNA-DIRECTED RNA POLYMERASE P3D
DE   (EC 2.7.7.48)].
OS   Encephalomyocarditis virus (strain emc-d diabetogenic).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Picornaviridae;
OC   Cardiovirus.
OX   NCBI_TaxID=12106;
RN   [1]
RP   SEQUENCE FROM N.A.
RX   MEDLINE=89243189; PubMed=2541543;
RA   Bae Y.S., Eun H.M., Yoon J.W.;
RT   "Genomic differences between the diabetogenic and nondiabetogenic
RT   variants of encephalomyocarditis virus.";
RL   Virology 170:282-287(1989).
CC   -!- FUNCTION: P3C POLYPEPTIDE IS A PROTEASE THAT CLEAVES AT CERTAIN
CC       Q/G SITES IN THE POLYPROTEIN. IT MAY BE A CYSTEINE PROTEASE.
CC   -!- SUBUNIT: THE VIRUS CAPSID IS COMPOSED OF 60 ICOSAHEDRAL UNITS,
CC       EACH OF WHICH IS COMPOSED OF ONE COPY EACH OF PROTEINS VP1, VP2,
CC       VP3, AND VP4.
CC   -!- PTM: SPECIFIC ENZYMATIC CLEAVAGES IN VIVO YIELD MATURE PROTEINS.
CC   -!- SIMILARITY: THE PROTEASE BELONGS TO PEPTIDASE FAMILY C3.
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DR   EMBL; M22458; AAA43034.1; -.
DR   PIR; A31473; GNNYED.
DR   HSSP; P12296; 1MEC.
DR   MEROPS; C03.009; -.
DR   MEROPS; U29.001; -.
DR   INTERPRO; IPR000605; -.
DR   INTERPRO; IPR001205; -.
DR   INTERPRO; IPR001676; -.
DR   PFAM; PF00680; RNA_dep_RNA_pol; 1.
DR   PFAM; PF00910; RNA_helicase; 1.
DR   PFAM; PF00073; rhv; 3.
KW   Polyprotein; Coat protein; Core protein; Transferase;
KW   RNA-directed RNA polymerase; Hydrolase; Thiol protease; Myristate.
FT   PROPEP        1     67       LEADER PEPTIDE.
FT   CHAIN        68    137       COAT PROTEIN VP4 (RHO).
FT   CHAIN       138    393       COAT PROTEIN VP2 (BETA).
FT   CHAIN       394    624       COAT PROTEIN VP3 (GAMMA).
FT   CHAIN       625    901       COAT PROTEIN VP1 (ALPHA).
FT   CHAIN       902   1058       CORE PROTEIN P2A (G).
FT   CHAIN      1059   1194       CORE PROTEIN P2B (I).
FT   CHAIN      1195   1519       CORE PROTEIN P2C (F).
FT   CHAIN      1520   1607       CORE PROTEIN P3A.
FT   CHAIN      1608   1627       GENOME-LINKED PROTEIN VPG (H).
FT   CHAIN      1628   1832       PICORNAIN 3C (P22).
FT   CHAIN      1833   2292       RNA-DIRECTED RNA POLYMERASE P3D (E).
FT   LIPID        68     68       MYRISTATE (BY SIMILARITY).
FT   ACT_SITE   1786   1786       PROTEASE (POTENTIAL).
FT   ACT_SITE   1804   1804       PROTEASE (POTENTIAL).
SQ   SEQUENCE   2292 AA;  255426 MW;  F2B0627B0F444107 CRC64;
     MATTMEQEIC AHSLTFKGCP KCSALQYRNG FYLLKYDEEW YPEELLTDGE DDVFDPELDM
     EVVFELQGNS TSSDKNNSSS DGNEGVIINN FYSNQYQNSI DLSANATGSD PPRTYGQFSN
     LLSGAVNAFS NMIPLLADQN TEEMENLSDR VLQDTAGNTV TNTQSTVGRL VGYGAVHDGE
     HPASCADTAS EKILAVERYY TFKVNDWTST QKPFEYIRIP LPHVLSGEDG GVFGAALRRH
     YLVKTGWRVQ VQCNASQFHA GSLLVFMAPE YPTLDAFAMD NRWSKDNLPN GTKTQTNRKG
     PFAMDHQNFW QWTLYPHQFL NLRTNTTVDL EVPYVNIAPT SSWTQHASWT LVIAVVAPLT
     YSTGASTSLD ITASIQPVRP VFNGLRHETL SRQSPIPVTI REHAGTWYST LPDSTVPIYG
     KTPVAPANYM VGEYKDFLEI AQIPTFIGNK IPNAVPYIEA SNTAVKTQPL ATYQVTLSCS
     CLANTFLAAL SRNFAQYRGS LVYTFVFTGT AMMKGKFLIA YTPPGAGKPT SRDQAMQATY
     AIWDLGLNSS YSFTVPFISP THFRMVGTDQ VNITNVDGWV TVWQLTPLTY PPGCPTSAKI
     LTMVSAGKDF SLKMPISPAP WSPQGVENAE RGVTEDTDAT ADFVAQPVYL PENQTKVAFF
     YDRSSPIGAF AVKSGSLESG FAPFSNETCP NSVILTPGPQ FDPAYDQLRP QRLTEIWGNG
     NEETSKVFPL KSKQDYSFCL FSPFVYYKCD LEVTLSPHTS GNHGLLVRWC PTGTPAKPTT
     QVLHEVSSLS EGRTPQVYSA GPGISNQISF VVPYNSPLSV LPAVWYNGHK RFDNTGSLGI
     APNSDFGTLF FAGTKPDIKF TVYLRYKNMR VFCPRPTVFF PWPSSGDKID MTPRAGVLML
     ESPNALDISR TYPTLHILIQ FNHGGLEIRL FRHGMFWAEA HADVILRSRT KQISFLNNGS
     FPSMDARAPW NPWKNTYHAV LRAEPYRVTM DVYHKRIRPF RLPLVQKEWN VREENVFGLY
     GIFNAHYAGY FADLLIHDIE TNPGPFMAKP KKQVFQTQGA AVSSMAQTLL PNDLASKVMG
     SAFTALLDAN EDAQKAMRII KTLSSLSDAW ENVKETLNNP EFWKQLLSRC VQLIAGMTIA
     VMHPDPLTLL CLGTLTAAEI TSQTSLCEEI VAKFKKIFTT PPPRFPTISL FQQQSPLKQV
     NDVFSLAKNL DWAVKTVEKV VDWFGTWVVQ EEKEQTLDQL LQRFPEHAKR ISDLRNGMSA
     YVECKESFDF FEKLYNQAVK EKRTGIAAVC EKFRQKHDHA TARCEPVVIV LRGDAGQGKS
     LSSQVIAQAV SKTIFGRQSV YSLPPDSDFF DGYENQFAAI MDDLGQNPDG SDFTTFCQMV
     STTNFLPNMA SLERNGTPFT SQIVVATTNL PEFRPVTIAH YPAVERRITF DYSVSAGPVC
     SKTEAGYKVL DVERAFRPTG DAPLPCFQNN CLFLEKAGLQ FRDNRTKEIL SLVDVIERAV
     ARIERKKKVL TTVQTLVAQA PVDEVSFHSV VQQLKARQEA TDEQLEELQE AFAKTQERSS
     VFSDWMKISA MLCAATLALS QVVKMAKTVK QMVRPDLVRV QLDEQEQGPY NEAVRAKPKT
     LQLLDIQGPN PVMDFEKYVA KFVTAPIDFV YPTGVSTQTC LLVKGRTLAV NRHMAESDWS
     SIVVRGVTHA RSTVRILAIA KAGKETDVSF IRLSSGPLFR DNTSKFVKAD DVLPATSAPV
     IGIMNTDIPM MFTGTFLKAG VSVPVETGQT FNHCIHYKAN TRKGWCGSAL LADLGGKKKI
     LGMHSAGSMG RTAASIVSQE MICAVVSAFE PQGALERLPD GPRIHVPRKT ALRPTVARRV
     FQPAYAPAVL SKFDPRTEAD VDEVAFSKHT SNQESLPPVF RMVAKEYANR VFTLLGRDNG
     RLTVKQALEG LEGMDPMDKN TSPGLPYTAL GMRRTDVVDW ESATLIPYAA DRLKKMNEGD
     FSDIVYQTFL KDELRPVEKV QAAKTRIVDV PPFEHCILGR QLLGRFASKF QTQPGLELGS
     AIGCDPDVHW TAFGVAMQGF ERVYDVDYSN FDSTHSVAMF RLLAEEFFTP ENGFDPLVKE
     YLESLAISTH AFEEKRYLIT GGLPSGCAAT SMLNTIMNNI IIRAGLYLTY KNFEFDDVKV
     LSYGDDLLVA TNYQLNFDKV RASLAKTGYK ITPANKTSTF PLDSTLEDVV FLKRKFKKEG
     PLYRPVMNRE ALEAMLSYYR PGTLSEKLTS ITMLAVHSGK PEYDRLFAPF REVGVVVPSF
     ESVEYRWRSL FW
//