ID NEF_HV1B1 STANDARD; PRT; 123 AA. AC P03404; DT 21-JUL-1986 (Rel. 01, Created) DT 13-AUG-1987 (Rel. 05, Last sequence update) DT 01-JUL-1993 (Rel. 26, Last annotation update) DE NEGATIVE FACTOR (F-PROTEIN) (27 KDA PROTEIN) (3'ORF). GN NEF. OS Human immunodeficiency virus type 1 (BH10 isolate) (HIV-1). OC Viruses; Retroid viruses; Retroviridae; Lentivirus. OX NCBI_TaxID=11678; RN [1] RP SEQUENCE FROM N.A. RX MEDLINE=85111123; PubMed=2578615; RA Ratner L., Haseltine W., Patarca R., Livak K.J., Starcich B.R., RA Josephs S.F., Doran E.R., Rafalski J.A., Whitehorn E.A., RA Baumeister K., Ivanoff L., Petteway S.R. Jr., Pearson M.L., RA Lautenberger J.A., Papas T.S., Ghrayeb J., Chang N.T., Gallo R.C., RA Wong-Staal F.; RT "Complete nucleotide sequence of the AIDS virus, HTLV-III."; RL Nature 313:277-284(1985). RN [2] RP POST-TRANSLATIONAL MODIFICATIONS, AND FUNCTION. RX MEDLINE=88039140; PubMed=3118220; RA Guy B., Kieny M.-P., Riviere Y., le Peuch C., Dott K., Girard M., RA Montagnier L., Lecocq J.-P.; RT "HIV F/3' orf encodes a phosphorylated GTP-binding protein resembling RT an oncogene product."; RL Nature 330:266-269(1987). CC -!- FUNCTION: NEF HAS GTPASE, GTP-BINDING AND AUTOPHOSPHORYLATING CC ACTIVITIES, IT SEEM TO DOWN REGULATE THE CD4(T4) ANTIGEN. CC -!- MISCELLANEOUS: IN THIS ISOLATE A MUTATION IN POSITION 124 ADD A CC STOP CODON AND THE PROTEIN IS MUCH SHORTER THEN IN OTHER ISOLATES CC (210 AA). CC -------------------------------------------------------------------------- CC This SWISS-PROT entry is copyright. It is produced through a collaboration CC between the Swiss Institute of Bioinformatics and the EMBL outstation - CC the European Bioinformatics Institute. There are no restrictions on its CC use by non-profit institutions as long as its content is in no way CC modified and this statement is not removed. Usage by and for commercial CC entities requires a license agreement (See http://www.isb-sib.ch/announce/ CC or send an email to license@isb-sib.ch). CC -------------------------------------------------------------------------- DR EMBL; M15654; AAA44206.1; -. DR PIR; A04005; ASLJH3. DR HSSP; Q70627; 2NEF. DR HIV; M15654; NEF$BH102. DR INTERPRO; IPR001558; -. DR PFAM; PF00469; F-protein; 1. KW AIDS; Myristate; GTP-binding. FT LIPID 2 2 MYRISTATE. SQ SEQUENCE 123 AA; 13606 MW; 0811735345F0EB8B CRC64; MGGKWSKSSV VGWPAVRERM RRAEPAADGV GAASRDLEKH GAITSSNTAA NNADCAWLEA QEEEEVGFPV TPQVPLRPMT YKAAVDLSHF LKEKGGLEGL IHSQRRQDIL DLWIYHTQGY FPD //