ID   MACS_RAT       STANDARD;      PRT;   308 AA.
AC   P30009;
DT   01-APR-1993 (Rel. 25, Created)
DT   01-APR-1993 (Rel. 25, Last sequence update)
DT   01-APR-1993 (Rel. 25, Last annotation update)
DE   MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE (MARCKS).
GN   MACS.
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   SEQUENCE FROM N.A.
RC   TISSUE=BRAIN;
RX   MEDLINE=91201362; PubMed=1707878;
RA   Erusalimsky J.D., Brooks S.F., Herget T., Morris C., Rozengurt E.;
RT   "Molecular cloning and characterization of the acidic 80-kDa protein
RT   kinase C substrate from rat brain. Identification as a glycoprotein.";
RL   J. Biol. Chem. 266:7073-7080(1991).
RN   [2]
RP   PHOSPHORYLATION SITES.
RC   TISSUE=BRAIN;
RX   MEDLINE=93135774; PubMed=8422248;
RA   Heemskerk F.M., Chen H.C., Huang F.L.;
RT   "Protein kinase C phosphorylates Ser-152, Ser-156 and Ser-163 but not
RT   Ser-160 of MARCKS in rat brain.";
RL   Biochem. Biophys. Res. Commun. 190:236-241(1993).
CC   -!- FUNCTION: MARCKS IS THE MOST PROMINENT CELLULAR SUBSTRATE FOR
CC       PROTEIN KINASE C. THIS PROTEIN BINDS CALMODULIN, ACTIN, AND
CC       SYNAPSIN. MARCKS IS A FILAMENTOUS (F) ACTIN CROSS-LINKING PROTEIN.
CC   -!- PTM: PHOSPHORYLATION BY PKC DISPLACES MARCKS FROM THE MEMBRANE. IT
CC       ALSO INHIBITS THE F-ACTIN CROSS-LINKING ACTIVITY.
CC   -!- SIMILARITY: BELONGS TO THE MARCKS FAMILY.
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DR   EMBL; M59859; -; NOT_ANNOTATED_CDS.
DR   PIR; A39773; A39773.
DR   INTERPRO; IPR002101; -.
DR   PFAM; PF02063; MARCKS; 1.
DR   PRINTS; PR00963; MARCKS.
DR   PROSITE; PS00826; MARCKS_1; 1.
DR   PROSITE; PS00827; MARCKS_2; 1.
KW   Phosphorylation; Myristate; Calmodulin-binding; Actin-binding;
KW   Membrane.
FT   INIT_MET      0      0       BY SIMILARITY.
FT   LIPID         1      1       MYRISTATE (BY SIMILARITY).
FT   DOMAIN      144    168       CALMODULIN-BINDING (PSD).
FT   MOD_RES     151    151       PHOSPHORYLATION (BY PKC).
FT   MOD_RES     155    155       PHOSPHORYLATION (BY PKC).
FT   MOD_RES     162    162       PHOSPHORYLATION (BY PKC).
SQ   SEQUENCE   308 AA;  29663 MW;  59B50228BB1B75D2 CRC64;
     GAQFSKTAAK GEAAAERPGE AAVASSPSKA NGQENGHVKV NGDASPAAAE PGAKEELQAN
     GSAPAADKEE PASGGAATPA AADKDEAAAA PEPGAATADK EAAEAEPAEP GSPSAETEGA
     SASSTSSPKA EDGAAPSPSS ETPKKKKKRF SFKKSFKLSG FSFKKSKKEA GEGAEAEGAT
     ADGAKDEAAA AAGGDAAAAP GEQAGGAGAE GAEGGESREA EAAEPEQPEQ PEQPAAEEPR
     AEEPSEAVGE KAEEPAPGAT ADDAPSAAGP EQEAPAATDE PAASAAPSAS PEPQPECSPE
     APPAPVAE
//