ID   GBI1_RAT       STANDARD;      PRT;   353 AA.
AC   P10824;
DT   01-JUL-1989 (Rel. 11, Created)
DT   01-OCT-1994 (Rel. 30, Last sequence update)
DT   15-JUL-1999 (Rel. 38, Last annotation update)
DE   GUANINE NUCLEOTIDE-BINDING PROTEIN G(I), ALPHA-1 SUBUNIT (ADENYLATE
DE   CYCLASE-INHIBITING G ALPHA PROTEIN).
GN   GNAI1 OR GNAI-1.
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   SEQUENCE FROM N.A.
RX   MEDLINE=88007678; PubMed=2820999;
RA   Jones D.T., Reed R.R.;
RT   "Molecular cloning of five GTP-binding protein cDNA species from rat
RT   olfactory neuroepithelium.";
RL   J. Biol. Chem. 262:14241-14249(1987).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RX   MEDLINE=94353239; PubMed=8073283;
RA   Coleman D.E., Berghuis A.M., Lee E., Linder M.E., Gilman A.G.,
RA   Sprang S.R.;
RT   "Structures of active conformations of Gi alpha 1 and the mechanism of
RT   GTP hydrolysis.";
RL   Science 265:1405-1412(1994).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF HETEROTRIMER.
RX   MEDLINE=96107343; PubMed=8521505;
RA   Wall M.A., Coleman D.E., Lee E., Iniguez-Lluhi J.A., Posner B.A.,
RA   Gilman A.G., Sprang S.R.;
RT   "The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma
RT   2.";
RL   Cell 83:1047-1058(1995).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF COMPLEX WITH RGS4.
RX   MEDLINE=97262066; PubMed=9108480;
RA   Tesmer J.J., Berman D.M., Gilman A.G., Sprang S.R.;
RT   "Structure of RGS4 bound to AlF4 -- activated G(i alpha1):
RT   stabilization of the transition state for GTP hydrolysis.";
RL   Cell 89:251-261(1997).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX   MEDLINE=98447508; PubMed=9772163;
RA   Coleman D.E., Sprang S.R.;
RT   "Crystal structures of the G protein Gi alpha 1 complexed with GDP and
RT   Mg2+: a crystallographic titration experiment.";
RL   Biochemistry 37:14376-14385(1998).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 31-345.
RX   MEDLINE=98371012; PubMed=9705312;
RA   Posner B.A., Mixon M.B., Wall M.A., Sprang S.R., Gilman A.G.;
RT   "The A326S mutant of Gialpha1 as an approximation of the receptor-
RT   bound state.";
RL   J. Biol. Chem. 273:21752-21758(1998).
CC   -!- FUNCTION: GUANINE NUCLEOTIDE-BINDING PROTEINS (G PROTEINS) ARE
CC       INVOLVED AS MODULATORS OR TRANSDUCERS IN VARIOUS TRANSMEMBRANE
CC       SIGNALING SYSTEMS.
CC   -!- FUNCTION: THE G(I) PROTEINS ARE INVOLVED IN HORMONAL REGULATION OF
CC       ADENYLATE CYCLASE: THEY INHIBIT THE CYCLASE IN RESPONSE TO BETA-
CC       ADRENERGIC STIMULI.
CC   -!- SUBUNIT: G PROTEINS ARE COMPOSED OF 3 UNITS (ALPHA, BETA & GAMMA).
CC       THE ALPHA CHAIN CONTAINS THE GUANINE NUCLEOTIDE BINDING SITE.
CC   -!- SIMILARITY: BELONGS TO THE G-ALPHA FAMILY. SUBFAMILY 1
CC       (G(I/O/T/Z)).
CC   --------------------------------------------------------------------------
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CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use  by  non-profit  institutions as long  as its content  is  in  no  way
CC   modified and this statement is not removed.  Usage  by  and for commercial
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DR   EMBL; M17527; AAA40825.1; -.
DR   PIR; C27423; RGRTI1.
DR   PDB; 1GIA; 30-SEP-94.
DR   PDB; 1GIL; 14-FEB-95.
DR   PDB; 1GIT; 12-FEB-97.
DR   PDB; 1GFI; 15-MAY-95.
DR   PDB; 1AGR; 16-JUN-97.
DR   PDB; 1GDD; 27-NOV-95.
DR   PDB; 1GG2; 12-FEB-97.
DR   PDB; 1GP2; 12-FEB-97.
DR   PDB; 1AS0; 12-NOV-97.
DR   PDB; 1AS2; 12-NOV-97.
DR   PDB; 1AS3; 12-NOV-97.
DR   PDB; 1BH2; 25-NOV-98.
DR   PDB; 1BOF; 16-FEB-99.
DR   PDB; 1CIP; 09-APR-99.
DR   INTERPRO; IPR001019; -.
DR   INTERPRO; IPR001408; -.
DR   PFAM; PF00503; G-alpha; 1.
DR   PRINTS; PR00318; GPROTEINA.
DR   PRINTS; PR00441; GPROTEINAI.
KW   GTP-binding; Transducer; Multigene family; ADP-ribosylation;
KW   Myristate; 3D-structure.
FT   INIT_MET      0      0       BY SIMILARITY.
FT   LIPID         1      1       MYRISTATE (BY SIMILARITY).
FT   NP_BIND      39     46       GTP (BY SIMILARITY).
FT   NP_BIND     199    202       GTP (BY SIMILARITY).
FT   NP_BIND     268    271       GTP (BY SIMILARITY).
FT   MOD_RES     177    177       ADP-RIBOSYL[1] (BY ACTION OF CTX).
FT   MOD_RES     350    350       ADP-RIBOSYL[1] (BY ACTION OF IAP).
SQ   SEQUENCE   353 AA;  40214 MW;  B23724E187E90A6D CRC64;
     GCTLSAEDKA AVERSKMIDR NLREDGEKAA REVKLLLLGA GESGKSTIVK QMKIIHEAGY
     SEEECKQYKA VVYSNTIQSI IAIIRAMGRL KIDFGDAARA DDARQLFVLA GAAEEGFMTA
     ELAGVIKRLW KDSGVQACFN RSREYQLNDS AAYYLNDLDR IAQPNYIPTQ QDVLRTRVKT
     TGIVETHFTF KDLHFKMFDV GGQRSERKKW IHCFEGVTAI IFCVALSDYD LVLAEDEEMN
     RMHESMKLFD SICNNKWFTD TSIILFLNKK DLFEEKIKKS PLTICYPEYA GSNTYEEAAA
     YIQCQFEDLN KRKDTKEIYT HFTCATDTKN VQFVFDAVTD VIIKNNLKDC GLF
//