ID   GBA1_YEAST     STANDARD;      PRT;   471 AA.
AC   P08539;
DT   01-AUG-1988 (Rel. 08, Created)
DT   01-OCT-1994 (Rel. 30, Last sequence update)
DT   15-DEC-1998 (Rel. 37, Last annotation update)
DE   GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP1-ALPHA).
GN   GPA1 OR SCG1 OR CDC70 OR DAC1 OR YHR005C.
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Ascomycota; Saccharomycetes; Saccharomycetales;
OC   Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   SEQUENCE FROM N.A.
RX   MEDLINE=87175629; PubMed=3031665;
RA   Nakafuku M., Itoh H., Nakamura S., Kaziro Y.;
RT   "Occurrence in Saccharomyces cerevisiae of a gene homologous to the
RT   cDNA coding for the alpha subunit of mammalian G proteins.";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:2140-2144(1987).
RN   [2]
RP   SEQUENCE FROM N.A.
RX   MEDLINE=87301743; PubMed=3113738;
RA   Dietzel C., Kurjan J.;
RT   "The yeast SCG1 gene: a G alpha-like protein implicated in the a- and
RT   alpha-factor response pathway.";
RL   Cell 50:1001-1010(1987).
RN   [3]
RP   SEQUENCE FROM N.A.
RC   STRAIN=S288C / AB972;
RX   MEDLINE=94378003; PubMed=8091229;
RA   Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J.,
RA   Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J.,
RA   Kucaba T., Hillier L., Jier M., Johnston L., Langston Y.,
RA   Latreille P., Louis E.J., Macri C., Mardis E., Menezes S., Mouser L.,
RA   Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K.,
RA   Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R.,
RA   Vaudin M.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   VIII.";
RL   Science 265:2077-2082(1994).
RN   [4]
RP   MYRISTOYLATION.
RX   MEDLINE=92038987; PubMed=1936988;
RA   Stone D.E., Cole G.M., de Barros Lopes M., Goebl M., Reed S.I.;
RT   "N-myristoylation is required for function of the pheromone-responsive
RT   G alpha protein of yeast: conditional activation of the pheromone
RT   response by a temperature-sensitive N-myristoyl transferase.";
RL   Genes Dev. 5:1969-1981(1991).
RN   [5]
RP   3D-STRUCTURE MODELING.
RA   Gaitatzes C.G., Neer E.J., Smith T.F.;
RL   Submitted (FEB-1998) to the PDB data bank.
CC   -!- FUNCTION: MEDIATES MATING PHEROMONE SIGNAL TRANSDUCTION BY
CC       INTERACTING WITH THE TRANSMEMBRANE RECEPTORS AND THE COGNATE BETA
CC       AND GAMMA SUBUNITS. THE ALPHA CHAIN IN RESPONSE TO THE PHEROMONE-
CC       BOUND RECEPTORS STE2/STE3 IS BELIEVED TO RELEASE THE BETA/GAMMA
CC       COMPLEX.
CC   -!- SUBUNIT: G PROTEINS ARE COMPOSED OF 3 UNITS (ALPHA, BETA & GAMMA).
CC       THE ALPHA CHAIN CONTAINS THE GUANINE NUCLEOTIDE BINDING SITE.
CC   -!- DOMAIN: CONTAINS AN 'INSERTION' SEQUENCE OF 109 RESIDUES WHICH IS
CC       NOT PRESENT IN OTHER G-PROTEIN ALPHA CHAINS.
CC   -!- SIMILARITY: BELONGS TO THE G-ALPHA FAMILY. SUBFAMILY 3 (G(Q)).
CC   --------------------------------------------------------------------------
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DR   EMBL; M15867; AAA34650.1; -.
DR   EMBL; M17414; AAA18403.1; -.
DR   EMBL; U10555; AAB68432.1; -.
DR   PIR; A25906; A25906.
DR   PDB; 1SCG; 17-JUN-98.
DR   SGD; S0001047; GPA1.
DR   INTERPRO; IPR001019; -.
DR   INTERPRO; IPR002975; -.
DR   PFAM; PF00503; G-alpha; 1.
DR   PRINTS; PR00318; GPROTEINA.
DR   PRINTS; PR01241; GPROTEINAFNG.
KW   GTP-binding; Transducer; Multigene family; Pheromone response;
KW   Myristate; 3D-structure.
FT   INIT_MET      0      0
FT   LIPID         1      1       MYRISTATE.
FT   NP_BIND      47     54       GTP (BY SIMILARITY).
FT   NP_BIND     318    321       GTP (BY SIMILARITY).
FT   NP_BIND     387    390       GTP (BY SIMILARITY).
FT   DOMAIN      126    234       INSERTION; NOT PRESENT IN OTHER G-
FT                                PROTEINS.
FT   CONFLICT     81     81       W -> R (IN REF. 2).
FT   CONFLICT    193    193       A -> V (IN REF. 2).
FT   CONFLICT    225    225       R -> K (IN REF. 2).
FT   CONFLICT    245    245       K -> R (IN REF. 2).
FT   CONFLICT    468    468       I -> S (IN REF. 2).
SQ   SEQUENCE   471 AA;  53944 MW;  1B0804236EF2B37E CRC64;
     GCTVSTQTIG DESDPFLQNK RANDVIEQSL QLEKQRDKNE IKLLLLGAGE SGKSTVLKQL
     KLLHQGGFSH QERLQYAQVI WADAIQSMKI LIIQARKLGI QLDCDDPINN KDLFACKRIL
     LKAKALDYIN ASVAGGSDFL NDYVLKYSER YETRRRVQST GRAKAAFDED GNISNVKSDT
     DRDAETVTQN EDADRNNSSR INLQDICKDL NQEGDDQMFV RKTSREIQGQ NRRNLIHEDI
     AKAIKQLWNN DKGIKQCFAR SNEFQLEGSA AYYFDNIEKF ASPNYVCTDE DILKGRIKTT
     GITETEFNIG SSKFKVLDAG GQRSERKKWI HCFEGITAVL FVLAMSEYDQ MLFEDERVNR
     MHESIMLFDT LLNSKWFKDT PFILFLNKID LFEEKVKSMP IRKYFPDYQG RVGDAEAGLK
     YFEKIFLSLN KTNKPIYVKR TCATDTQTMK FVLSAVTDLI IQQNLKKIGI I
//