comparison: overlap of the big-Pi plant predictions in Arabidopsis thaliana with the list created by Borner GHH et al. (2003)

Contact:
Birgit Eisenhaber (IMP/Austria)
Michael Wildpaner (IMP/Austria)
Frank Eisenhaber (IMP/Austria)
Carolyn Schultz (University of Adelaide/Australia)
Paul Dupree and Georg Borner (University of Cambridge/UK)

Summary Table:

	Borner et al. 2003	Eisenhaber et al. 2003
Number of proteins with predicted GPI modification and predicted N-terminal Signal Peptide	248	187
Number of proteins contained in both lists	165	165
Number of proteins which are predicted as GPI candidates by the big-Pi program but not having a predicted Signal Peptide by SignalP version 1.2	6(*)
Number of proteins not in the release June 2002 of the A.thaliana genome	(14)
Number of proteins not in the release June 2002 of the A.thaliana genome predicted by the big-Pi program as GPI candidates	10
Number of proteins that are predicted to be GPI modified only by Borner et al.	67(**)
Number of proteins that are predicted to be GPI modified only by Eisenhaber et al.		22

(*) The N-terminus of 5 of these sequences has been corrected, and a N-terminal signal leader peptide is predicted now.
(**) Now 11 of these proteins are predicted by the big-Pi plant program as suitable candidates for GPI-anchoring due to corrections of their C-termini.

The A.thaliana proteins which are predicted as suitable GPI candidates only with the big-Pi plant program are listed in detail:

AGP related (classical AGPs, AG-peptides, Lys-rich, fasciclin-like)

At5g16920-68299.m01758

Glycohydrolases(families 1,5,8 or 17; PF00232, PF00150, PF01501, PF00322)

At1g11820-68300.m08280

At4g13600-68296.m01383

Pectin metabolism relaed

At3g17220-68298.m01892

Proteases

aspartyl proteases (PF00026)

At3g51340-68298.m04821

LTP family/seed storage/protease inhibitor (PF00234)

At1g73560-68300.m07410

At2g13820-68297.m01269

At3g22620-68298.m02494

Kazal-type serine protease inhibitors (PF00050)

At4g01575-68296.m00178

Signalling receptor-like

At2g25790-68297.m02632

At5g67130-68299.m06773

At2g36500-68297.m03826

Cation transporters

At1g68100-68300.m06759

At2g32270-68297.m03363

Other functions

At5g01990-68299.m00105

At3g20590-68298.m02264

At3g20600-68298.m02265

At5g42590-68299.m03960

Hypothetical proteins/unknown function

At2g21180-68297.m02125

At4g29735-68296.m03228

At4g30670-68296.m03330

At4g30770-68296.m03340