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Comparison: application of the big-Pi plant predictor on the list created by Borner GHH et al. , Plant Physiol 2003, in press

Contact:
Birgit Eisenhaber (IMP/Austria)
Michael Wildpaner (IMP/Austria)
Frank Eisenhaber (IMP/Austria)
Carolyn Schultz (University of Adelaide/Australia)
Paul Dupree and Georg Borner (University of Cambridge/UK)

Summary Table:

  Prediction Quality Rate Percentage
1:P 171/248 68.95%
1:S 21/248 8.47%
1:S (Positive Total Score) 14/248 5.65%
1:S (Negative Total Score) 7/248 2.82%
1:I 36/248 14.52%
1:N 20/248 8.06%
Not Predicted 63/248 25.40%
Predicted 185/248 74.60%

With a relaxed definition of prediction by big-Pi, all P and S cases, 192 out of 248 sequences (77.42%) would be suggested as suitable candidates for GPI anchoring.

Reasons for not predicted proteins are detailed below

N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
1 At5g64310 131 1:P 108 11.81 -0.06 11.75 4.588110e-06
2 At2g22470 131 1:P 107 20.93 -1.04 19.89 3.425805e-08
3 At4g40091 139 1:P 116 13.57 -0.86 12.71 2.799657e-06
4 At5g10430 135 1:P 111 22.16 -2.16 20.00 3.160536e-08
5 At1g35230 133 1:P 109 20.29 -1.03 19.26 5.297011e-08
6 At5g14380 150 1:P 126 17.29 -6.07 11.22 5.972834e-06
7 At5g65390 130 1:P 106 19.68 -1.05 18.63 8.158456e-08
8 At2g14890 191 1:P 172 12.83 -2.62 10.21 9.768812e-06
9 At4g09030 127 1:P 107 15.92 -2.50 13.42 1.913372e-06
10 At3g01700 136 1:P 112 15.76 -3.48 12.28 3.494692e-06
11 At2g23130 185 1:P 159 11.66 -1.76 9.90 1.128687e-05
12 At4g37450 209 1:P 183 16.19 -2.13 14.06 1.346535e-06
13 At1g68725 248 1:N 221 1.76 -5.90 -4.14 1.584030e-03
14 At5g18690 116 1:P 89 12.68 -0.72 11.96 4.115085e-06
15 At2g47930 136 1:P 108 19.68 -4.07 15.61 5.511601e-07
16 At3g06360 125 1:P 98 12.16 -4.68 7.48 3.317560e-05
17 At4g16985 164 1:P 138 16.39 -0.94 15.45 6.067501e-07
18 At3g13520 60 1:P 33 9.61 -7.02 2.59 2.144392e-04
19 At4g26320 59 1:P 38 15.31 -4.72 10.59 8.140760e-06
20 At5g56540 60 1:P 38 16.69 -6.90 9.79 1.187008e-05
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
21 At5g11740 61 1:P 35 21.13 -1.75 19.38 4.898819e-08
22 At1g55330 58 1:P 36 15.61 -2.53 13.08 2.291733e-06
23 At5g53250 63 1:P 38 12.46 -1.55 10.91 6.962598e-06
24 At3g57690 61 1:P 35 17.04 -4.17 12.87 2.574044e-06
25 At5g40730 69 1:P 42 15.54 -2.40 13.14 2.219345e-06
26 At3g01730 87 1:P 64 14.68 -1.30 13.38 1.956909e-06
27 At3g20865 62 1:P 35 20.41 -0.01 20.40 2.372561e-08
28 At2g46330 73 1:I 37 12.65 -21.25 -8.60 4.472549e-03
29 At3g61640 74 1:I 37 12.88 -25.27 -12.39 9.332184e-03
30 At5g11990 181 1:P 154 11.34 -3.48 7.86 2.822303e-05
31 At5g49280 162 1:P 139 15.97 -0.37 15.60 5.539111e-07
32 At3g06750 147 1:P 127 15.63 -1.22 14.41 1.106196e-06
33 At4g16140 164 1:P 141 11.63 -5.87 5.76 6.680693e-05
34 At1g70990 138 1:S 111 4.30 -5.47 -1.17 7.034085e-04
35 At1g23040 144 1:P 120 12.00 -1.04 10.96 6.810274e-06
36 At1g02405 134 1:P 111 5.65 -0.47 5.18 8.382686e-05
37 At5g26330 187 1:I 155 9.27 -14.25 -4.98 1.958295e-03
38 At2g31050 200 1:P 177 16.68 -2.33 14.35 1.145833e-06
39 At2g26720 206 1:P 178 10.56 -2.21 8.35 2.278833e-05
40 At5g20230 196 1:P 174 19.11 -1.50 17.61 1.596242e-07
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
41 At2g32300 261 1:P 232 7.00 -2.84 4.16 1.226142e-04
42 At2g44790 202 1:P 178 17.68 -0.84 16.84 2.596617e-07
43 At3g60280 222 1:P 198 15.97 -3.89 12.08 3.875946e-06
44 At3g60270 187 1:P 164 9.03 -2.06 6.97 4.109705e-05
45 At1g22480 174 1:N 148 1.06 -4.99 -3.93 1.502548e-03
46 At1g72230 181 1:P 153 13.61 -2.67 10.94 6.863952e-06
47 At3g27200 174 1:P 148 16.93 -1.10 15.83 4.820164e-07
48 At5g07475 192 1:P 161 11.87 -9.62 2.25 2.406465e-04
49 At2g25060 176 1:P 154 13.07 -3.79 9.28 1.504901e-05
50 At5g53870 370 1:P 345 14.87 -8.71 6.16 5.699746e-05
51 At4g30590 190 1:P 163 13.09 -1.42 11.67 4.779703e-06
52 At4g27520 349 1:P 326 14.52 -0.28 14.24 1.212966e-06
53 At4g31840 177 1:P 154 17.82 -1.55 16.27 3.697304e-07
54 At2g23990 207 1:P 182 15.08 -4.01 11.07 6.444261e-06
55 At3g20570 203 1:P 179 20.10 -1.39 18.71 7.724964e-08
56 At4g32490 216 1:P 187 12.33 -3.05 9.28 1.507796e-05
57 At5g25090 186 1:P 164 18.80 -1.51 17.29 1.964664e-07
58 At4g28365 199 1:P 179 16.23 -3.94 12.29 3.487477e-06
59 At3g01070 167 1:S 138 12.01 -11.74 0.27 4.560764e-04
60 At1g08500 228 1:P 208 14.80 -5.10 9.70 1.240284e-05
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
61 At1g79800 192 1:P 166 18.73 -4.00 14.73 9.214194e-07
62 At3g18590 188 1:P 171 12.96 -2.87 10.09 1.032879e-05
63 At1g48940 177 1:N 160 -0.89 -7.77 -8.66 4.526338e-03
64 At5g15350 172 1:I 142 4.29 -28.67 -24.38 5.016667e-02
65 At5g60920 456 1:P 431 19.08 -0.78 18.30 1.019147e-07
66 At3g02210 452 1:P 426 11.20 -5.08 6.12 5.793040e-05
67 At3g29810 441 1:P 417 17.15 -0.73 16.42 3.370452e-07
68 At5g15630 218 1:P 196 16.48 -1.03 15.45 6.062885e-07
69 At1g09790 442 1:P 417 18.80 -0.70 18.10 1.159778e-07
70 At3g16860 653 1:P 628 11.66 -3.67 7.99 2.666751e-05
71 At5g49270 663 1:P 638 10.00 -4.70 5.30 8.002897e-05
72 At3g20580 672 1:P 646 13.78 -8.38 5.40 7.711078e-05
73 At4g16120 661 1:I 637 6.02 -21.17 -15.15 1.486889e-02
74 At4g27110 717 1:P 685 10.74 -4.19 6.55 4.870229e-05
75 At5g55480 766 1:P 742 12.01 -4.49 7.52 3.267140e-05
76 At4g26690 759 1:P 735 13.91 -3.10 10.81 7.309804e-06
77 At5g58170 750 1:P 728 15.54 -0.44 15.10 7.444868e-07
78 At5g58050 753 1:S 728 11.20 -9.85 1.35 3.236966e-04
79 At1g66970 763 1:P 742 10.83 -4.02 6.81 4.382232e-05
80 At3g20520 729 1:I 701 7.34 -10.33 -2.99 1.172831e-03
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
81 At1g74790 695 1:P 665 17.92 -1.18 16.74 2.762479e-07
82 At5g62630 696 1:P 672 20.16 -1.86 18.30 1.016945e-07
83 At5g39970 690 1:P 663 13.65 -1.74 11.91 4.229057e-06
84 At5g55730 424 1:P 398 9.96 -1.81 8.15 2.494549e-05
85 At4g12730 403 1:N 378 3.00 -5.83 -2.83 1.122288e-03
86 At2g24450 280 1:P 256 20.10 -1.52 18.58 8.451990e-08
87 At3g46550 420 1:I 395 7.63 -15.00 -7.37 3.428513e-03
88 At4g31370 278 1:P 255 17.44 -1.08 16.36 3.501847e-07
89 At2g20520 247 1:I 220 13.55 -15.42 -1.87 8.591675e-04
90 At2g04780 254 1:S 232 12.19 -10.76 1.43 3.152760e-04
91 At2g45470 420 1:P 392 16.56 -1.34 15.22 6.940608e-07
92 At1g03870 247 1:P 224 14.59 -0.40 14.19 1.250229e-06
93 At3g60900 422 1:P 398 15.40 -4.96 10.44 8.730674e-06
94 At5g03170 246 1:P 219 11.44 -2.78 8.66 1.989181e-05
95 At5g60490 249 1:S 220 7.60 -5.82 1.78 2.811136e-04
96 At5g44130 247 1:P 224 15.69 -0.01 15.68 5.278481e-07
97 At3g12660 255 1:S 225 9.95 -8.47 1.48 3.102655e-04
98 At1g30800 239 1:P 216 17.73 -0.16 17.57 1.639646e-07
99 At4g12950 176 1:P 154 10.92 -3.82 7.10 3.887082e-05
100 At5g42720 438 1:P 409 13.07 -8.52 4.55 1.063198e-04
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
101 At5g42100 425 1:P 401 13.69 -0.14 13.55 1.784927e-06
102 At5g58090 477 1:P 455 14.12 -1.79 12.33 3.408535e-06
103 At5g61130 201 1:I 172 11.69 -13.47 -1.78 8.393127e-04
104 At1g77780 363 1:I 342 0.91 -29.32 -28.41 7.504540e-02
105 At5g58480 465 1:P 442 12.65 -3.81 8.84 1.838499e-05
106 At2g19440 478 1:P 451 5.92 -1.76 4.16 1.226344e-04
107 At1g26450 197 1:P 172 13.83 -0.56 13.27 2.076090e-06
108 At4g31140 484 1:P 460 9.07 -2.15 6.92 4.190108e-05
109 At5g64790 485 1:P 457 8.94 -4.80 4.14 1.235603e-04
110 At1g11830 511 1:P 487 5.88 -1.79 4.09 1.260176e-04
111 At5g08000 194 1:N 171 7.59 -14.90 -7.31 3.381889e-03
112 At1g69295 222 1:P 197 11.98 -1.62 10.36 9.094143e-06
113 At4g29360 534 1:S 507 12.48 -10.60 1.88 2.722004e-04
114 At1g64760 481 1:P 455 14.66 -1.90 12.76 2.718216e-06
115 At3g15800 399 1:P 369 19.06 -7.50 11.56 5.044693e-06
116 At5g56590 506 1:I 472 12.27 -12.07 0.20 4.670339e-04
117 At3g04010 491 1:P 465 14.75 -1.77 12.98 2.417144e-06
118 At5g20870 501 1:P 478 13.85 -2.30 11.55 5.073772e-06
119 At1g32860 419 1:S 399 5.27 -6.40 -1.13 6.962364e-04
120 At3g13560 505 1:P 473 7.54 -4.26 3.28 1.686170e-04
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
121 At2g01630 501 1:P 470 7.39 -3.41 3.98 1.309583e-04
122 At2g26600 388 1:P 366 12.03 -5.54 6.49 5.001563e-05
123 At3g58100 180 1:I 157 6.69 -22.19 -15.50 1.572471e-02
124 At1g66250 505 1:N 477 5.65 -14.29 -8.64 4.510517e-03
125 At3g24330 500 1:P 479 13.86 -2.84 11.02 6.593204e-06
126 At1g18650 184 1:S 158 10.20 -9.69 0.51 4.242757e-04
127 At5g18220 488 1:N 465 0.39 -7.23 -6.84 3.037574e-03
128 At3g15720 456 1:I 430 -6.46 -19.04 -25.50 5.647970e-02
129 At5g04310 475 1:S 450 5.82 -7.71 -1.89 8.647481e-04
130 At3g54920 501 1:P 474 12.82 -0.67 12.15 3.732833e-06
131 At3g53190 483 1:P 451 10.04 -5.35 4.69 1.007032e-04
132 At3g51330 529 1:P 504 9.30 -1.81 7.49 3.304501e-05
133 At3g51350 475 1:P 449 10.83 0.00 10.83 7.247040e-06
134 At3g02740 488 1:I 466 -1.03 -13.89 -14.92 1.433200e-02
135 At1g08210 492 1:P 466 11.97 -0.87 11.10 6.337929e-06
136 At5g36260 478 1:S 452 12.12 -10.76 1.36 3.233221e-04
137 At5g10080 528 1:P 503 16.07 -0.58 15.49 5.919264e-07
138 At4g35880 455 1:N 431 2.14 -6.58 -4.44 1.708298e-03
139 At1g05840 485 1:P 461 15.12 -7.10 8.02 2.637664e-05
140 At1g65240 475 1:S 449 9.03 -8.12 0.91 3.737808e-04
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
141 At2g17760 513 1:P 493 17.49 -0.99 16.50 3.217152e-07
142 At1g70170 378 1:P 356 9.79 -0.37 9.42 1.413705e-05
143 At1g24140 377 1:N 352 7.65 -12.79 -5.14 2.032900e-03
144 At1g59970 360 1:P 335 8.35 -3.92 4.43 1.110509e-04
145 At4g16640 364 1:N 338 4.69 -7.23 -2.54 1.037086e-03
146 At2g45040 342 1:S 317 5.21 -5.84 -0.63 6.007296e-04
147 At3g43960 376 1:P 350 16.49 -2.32 14.17 1.262525e-06
148 At4g08670 194 1:P 164 16.89 -5.83 11.06 6.490063e-06
149 At1g18280 180 1:P 158 20.09 -1.08 19.01 6.284218e-08
150 At5g09370 158 1:I 132 9.31 -9.46 -0.15 5.202092e-04
151 At2g48130 183 1:P 157 13.79 -2.39 11.40 5.483314e-06
152 At4g12360 161 1:P 139 9.76 -3.16 6.60 4.777802e-05
153 At2g27130 159 1:P 132 14.11 -3.03 11.08 6.411561e-06
154 At3g22611 203 1:P 181 19.11 -0.84 18.27 1.036879e-07
155 At2g44300 204 1:I 178 -1.69 -15.90 -17.59 2.148846e-02
156 At5g64080 173 1:P 144 16.52 -2.86 13.66 1.676305e-06
157 At2g48140 200 1:I 183 6.61 -8.78 -2.17 9.369757e-04
158 At1g05450 206 1:P 184 17.95 -2.76 15.19 7.040601e-07
159 At1g36150 256 1:P 232 16.62 -8.89 7.73 2.987202e-05
160 At1g62790 150 1:P 127 11.41 -1.16 10.25 9.573059e-06
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
161 At2g13830 169 1:P 147 14.92 -8.35 6.57 4.831245e-05
162 At3g43720 193 1:P 166 12.13 -2.43 9.70 1.242207e-05
163 At5g13900 151 1:I 120 8.02 -15.74 -7.72 3.700852e-03
164 At3g22600 170 1:P 146 12.77 -4.19 8.58 2.062659e-05
165 At1g73890 193 1:P 169 13.15 -2.27 10.88 7.058630e-06
166 At3g58550 177 1:P 152 10.73 -8.48 2.25 2.409041e-04
167 At4g22630 162 1:P 137 10.27 -5.76 4.51 1.078469e-04
168 At2g44290 205 1:I 179 -0.26 -13.19 -13.45 1.123253e-02
169 At4g22640 140 1:P 118 15.23 -0.04 15.19 7.054315e-07
170 At1g27950 193 1:P 160 19.25 -7.22 12.03 3.977965e-06
171 At4g14805 219 1:S 193 8.12 -7.63 0.49 4.262689e-04
172 At4g14815 156 1:P 128 10.63 -1.73 8.90 1.787854e-05
173 At1g55260 184 1:I 160 1.87 -20.27 -18.40 2.407998e-02
174 At5g51480 592 1:P 564 13.78 -4.19 9.59 1.302712e-05
175 At4g12420 587 1:P 562 16.93 -6.79 10.14 1.009186e-05
176 At4g25240 589 1:P 563 14.43 -4.90 9.53 1.342583e-05
177 At1g63550 323 1:N 293 9.59 -14.11 -4.52 1.743338e-03
178 At5g41280 286 1:P 261 11.40 -3.46 7.94 2.731906e-05
179 At5g41290 287 1:P 259 11.72 -3.45 8.27 2.360888e-05
180 At5g41300 287 1:P 263 6.63 -0.74 5.89 6.345912e-05
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
181 At1g63580 273 1:N 247 -5.21 -9.14 -14.35 1.307029e-02
182 At4g36010 301 1:P 276 12.22 -5.65 6.57 4.831037e-05
183 At1g20030 316 1:S 286 10.38 -9.21 1.17 3.440482e-04
184 At4g38660 345 1:N 322 -0.90 -8.96 -9.86 5.792026e-03
185 At1g07460 322 1:P 298 8.25 -2.66 5.59 7.155918e-05
186 At1g77630 409 1:I 374 8.01 -16.21 -8.20 4.108550e-03
187 At1g21880 416 1:P 391 13.99 -1.61 12.38 3.317849e-06
188 At2g17120 350 1:P 318 11.33 -0.76 10.57 8.223500e-06
189 At1g80080 496 1:P 469 8.61 -3.77 4.84 9.542634e-05
190 At2g42800 462 1:I 432 7.43 -16.71 -9.28 5.151579e-03
191 At4g28560 449 1:I 426 8.29 -13.90 -5.61 2.280696e-03
192 At1g10375 305 1:I 276 5.67 -27.90 -22.23 3.933212e-02
193 At5g14110 70 1:P 41 11.06 -3.82 7.24 3.669818e-05
194 At5g63500 69 1:P 40 9.98 -6.95 3.03 1.841470e-04
195 At3g01940 70 1:N 38 -6.74 -10.49 -17.23 2.039811e-02
196 At5g40980 69 1:I 42 -4.41 -16.34 -20.75 3.284112e-02
197 At5g40970 69 1:I 40 -4.23 -12.18 -16.41 1.806583e-02
198 At5g50660 67 1:I 39 -4.67 -12.93 -17.60 2.153183e-02
199 At3g01950 70 1:S 41 6.76 -6.86 -0.10 5.118038e-04
200 At5g40960 68 1:N 41 -3.48 -11.45 -14.93 1.435971e-02
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
201 At5g07190 213 1:P 184 19.96 -6.46 13.50 1.830506e-06
202 At5g62210 223 1:P 196 24.41 -0.71 23.70 1.858257e-09
203 At5g62200 190 1:P 160 9.86 -5.00 4.86 9.456895e-05
204 At3g07390 252 1:P 226 14.95 -0.66 14.29 1.181361e-06
205 At1g24520 119 1:P 88 14.12 -0.09 14.03 1.364848e-06
206 At4g15460 148 1:S 123 8.20 -7.47 0.73 3.949825e-04
207 At1g61900 421 1:P 393 7.63 -2.46 5.17 8.406813e-05
208 At4g28100 304 1:P 282 17.51 -6.50 11.01 6.639663e-06
209 At3g18050 335 1:N 306 3.80 -13.51 -9.71 5.616297e-03
210 At1g07135 155 1:P 128 11.83 -0.89 10.94 6.859959e-06
211 At3g04640 159 1:P 137 16.24 0.00 16.24 3.770785e-07
212 At1g29980 407 1:P 385 10.50 -5.09 5.41 7.660791e-05
213 At2g34510 401 1:P 382 12.73 -1.28 11.45 5.332528e-06
214 At4g28280 160 1:P 135 12.74 -1.62 11.12 6.292866e-06
215 At5g56170 168 1:P 144 13.85 -1.65 12.20 3.645513e-06
216 At2g20700 181 1:P 153 13.96 -1.17 12.79 2.672529e-06
217 At5g22430 172 1:N 151 8.08 -14.61 -6.53 2.829823e-03
218 At2g28410 115 1:I 90 0.37 -10.03 -9.66 5.555518e-03
219 At3g27410 132 1:P 108 19.61 -1.35 18.26 1.041891e-07
220 At1g09175 150 1:P 120 11.13 -2.26 8.87 1.812560e-05
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
221 At1g64640 191 1:I 166 16.77 -16.80 -0.03 5.002063e-04
222 At5g35890 287 1:I 263 -2.03 -23.40 -25.43 5.603864e-02
223 At5g14190 119 1:S 92 5.14 -5.65 -0.51 5.793993e-04
224 At3g61980 117 1:P 86 14.98 -0.08 14.90 8.362888e-07
225 At5g08210 67 1:P 46 5.47 -0.91 4.56 1.059625e-04
226 At5g16670 76 1:S 56 7.22 -6.12 1.10 3.518288e-04
227 At1g21090 242 1:P 215 12.97 -2.28 10.69 7.759542e-06
228 At1g70985 135 1:P 111 17.30 -0.86 16.44 3.335752e-07
229 At3g26110 128 1:P 104 16.35 -13.81 2.54 2.183158e-04
230 At2g29660 373 1:I 352 -12.61 -18.93 -31.54 9.863299e-02
231 At3g44100 152 1:N 130 5.72 -19.11 -13.39 1.111595e-02
232 At5g67131 426 1:P 404 10.71 -2.82 7.89 2.789712e-05
233 At4g28085 84 1:P 58 17.63 -0.27 17.36 1.873755e-07
234 At1g54860 200 1:P 174 8.74 -0.70 8.04 2.609657e-05
235 At3g06035 200 1:S 174 7.03 -7.25 -0.22 5.312490e-04
236 At5g19230 189 1:P 164 17.78 -15.13 2.65 2.102538e-04
237 At5g19250 196 1:P 171 19.12 -2.51 16.61 3.001951e-07
238 At5g26290 333 1:I 313 1.83 -22.00 -20.17 3.050817e-02
239 At5g26300 349 1:I 329 0.76 -25.32 -24.56 5.111011e-02
240 At5g40620 130 1:P 109 15.70 -0.05 15.65 5.364610e-07
N   ID Length Prediction Quality Site Profile Score Function Score Total Score PValue
241 At5g14150 383 1:S 362 3.74 -2.65 1.09 3.527080e-04
242 At3g24518 510 1:I 488 7.99 -8.68 -0.69 6.109457e-04
243 At3g55790 103 1:P 81 4.84 -0.97 3.87 1.362560e-04
244 At3g58890 293 1:I 275 -12.58 -13.61 -26.19 6.055774e-02
245 At1g56320 163 1:N 142 -4.10 -6.80 -10.90 7.085780e-03
246 At1g23050 161 1:P 128 11.94 -4.70 7.24 3.677897e-05
247 At4g38140 145 1:N 119 2.43 -5.94 -3.51 1.344145e-03
248 At4g14746 250 1:I 223 4.36 -13.48 -9.12 4.982361e-03



63 proteins described in the paper have not been predicted as suitable candidates for GPI anchoring:

  1. 2 proteins have an untypical N-terminal region [-11..-1] (relative to the omega-site):

    2. At2g45040 (class S with negative score)
    At3g24518

  2. We could not detect a suitable omega-site for 20 proteins:

    3. At1g68725 (profile)
    At2g46330 (motif length)
    At3g61640 (motif length)
    At1g22480 (profile, Cys at the omega-site)
    At4g16120
    At4g12730 (profile)
    At5g56590 (motif length)
    At5g18220
    At3g15720
    At4g16640
    At5g09370
    At2g44290
    At4g28560
    At3g01940
    At5g40970
    At3g01950 (class S with negative score)
    At5g40960
    At2g28410
    At1g56320
    At4g38140

  3. In further 6 proteins a typical spacer region is missing:

    4. At5g26330
    At1g48940
    At3g20520
    At4g35880
    At2g48140
    At4g14746

  4. 20 proteins lack an appropriately constructed hydrophobic tail:

    5. At1g70990 (class S with negative score)
    At5g15350
    At3g46550
    At2g20520
    At5g61130
    At1g77780
    At5g08000 (profile)
    At1g32860 (class S with negative score)
    At1g24140 (profile)
    At1g66250 (profile)
    At5g04310 (class S with negative score)
    At1g24140 (profile)
    At1g63550 (profile)
    At1g10375
    At3g18050 (profile)
    At5g22430
    At1g64640
    At5g14190 (class S with negative score)
    At3g44100
    At3g06035 (class S with negative score)

  5. 15 sequences are non-compatible with our prediction function at all:

    6. At3g02740
    At2g44300
    At5g13900
    At1g55260
    At1g63580
    At4g38660
    At1g77630
    At2g42800
    At5g40980
    At5g50660
    At5g35890
    At5g26290
    At5g26300
    At2g29660
    At3g58890



Last modified: 26th March 2003