ID   PPBI_RAT       STANDARD;      PRT;   540 AA.
AC   P15693;
DT   01-APR-1990 (REL. 14, CREATED)
DT   01-APR-1990 (REL. 14, LAST SEQUENCE UPDATE)
DT   01-OCT-1996 (REL. 34, LAST ANNOTATION UPDATE)
DE   ALKALINE PHOSPHATASE, INTESTINAL 1 PRECURSOR (EC 3.1.3.1) (IAP-I).
OS   RATTUS NORVEGICUS (RAT).
OC   EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; TETRAPODA; MAMMALIA;
OC   EUTHERIA; RODENTIA.
RN   [1]
RP   SEQUENCE FROM N.A., AND SEQUENCE OF 21-34 AND 287-300.
RX   MEDLINE; 90167110.
RA   LOWE M., STRAUSS A.W., ALPERS R., SEETHARAM S., ALPERS D.H.;
RL   BIOCHIM. BIOPHYS. ACTA 1037:170-177(1990).
RN   [2]
RP   GPI-ANCHOR.
RX   MEDLINE; 95263539.
RA   ENGLE M.J., MAHMOOD A., ALPERS D.H.;
RL   J. BIOL. CHEM. 270:11935-11940(1995).
CC   -!- CATALYTIC ACTIVITY: AN ORTHOPHOSPHORIC MONOESTER + H(2)O = AN
CC       ALCOHOL + ORTHOPHOSPHATE (AT A HIGH PH OPTIMUM).
CC   -!- SUBUNIT: HOMODIMER.
CC   -!- SUBCELLULAR LOCATION: ATTACHED TO THE MEMBRANE BY A GPI-ANCHOR.
CC   -!- IN MOST MAMMALS THERE ARE FOUR DIFFERENT ISOZYMES: PLACENTAL,
CC       PLACENTAL-LIKE, INTESTINAL AND TISSUE NON-SPECIFIC (LIVER/BONE/
CC       KIDNEY). RAT HAS TWO GENES FOR THE INTESTINAL ISOZYME.
CC   -!- SIMILARITY: STRONG TO ALL OTHER MAMMALIAN AP, AND TO BACTERIAL
CC       AND YEAST AP.
DR   EMBL; X17611; G57823; -.
DR   PIR; S08214; S08214.
DR   HSSP; P00634; 1ALK.
DR   PROSITE; PS00123; ALKALINE_PHOSPHATASE; 1.
KW   HYDROLASE; ZINC; MAGNESIUM; PHOSPHORYLATION; TRANSMEMBRANE;
KW   MULTIGENE FAMILY; GLYCOPROTEIN; GPI-ANCHOR; SIGNAL.
FT   SIGNAL        1     20
FT   CHAIN        21    511       ALKALINE PHOSPHATASE, INTESTINAL 1.
FT   PROPEP      512    540       REMOVED IN MATURE FORM (POTENTIAL).
FT   MOD_RES     112    112       PHOSPHORYLATION (BY SIMILARITY).
FT   ACT_SITE    112    112       BY SIMILARITY.
FT   LIPID       511    511       GPI-ANCHOR (POTENTIAL).
FT   CARBOHYD    142    142       POTENTIAL.
FT   CARBOHYD    301    301       POTENTIAL.
FT   CARBOHYD    428    428       POTENTIAL.
SQ   SEQUENCE   540 AA;  58402 MW;  AD7BA10F CRC32;
     MQGDWVLLLL LGLRIHLSFG VIPVEEENPV FWNQKAKEAL DVAKKLQPIQ TSAKNLILFL
     GDGMGVPTVT ATRILKGQLG GHLGPETPLA MDHFPFTALS KTYNVDRQVP DSAGTATAYL
     CGVKANYKTI GVSAAARFNQ CNSTFGNEVF SVMHRAKKAG KSVGVVTTTR VQHASPAGTY
     AHTVNRDWYS DADMPSSALQ EGCKDIATQL ISNMDIDVIL GGGRKFMFPK GTPDPEYPGD
     SDQSGVRLDS RNLVEEWLAK YQGTRYVWNR EQLMQASQDP AVTRLMGLFE PTEMKYDVNR
     NASADPSLAE MTEVAVRLLS RNPQGFYLFV EGGRIDQGHH AGTAYLALTE AVMFDSAIEK
     ASQLTNEKDT LTLITADHSH VFAFGGYTLR GTSIFGLAPL NAQDGKSYTS ILYGNGPGYV
     LNSGNRPNVT DAESGDVNYK QQAAVPLSSE THGGEDVAIF ARGPQAHLVH GVQEQNYIAH
     VMAFAGCLEP YTDCGLAPPA DENRPTTPVQ NSAITMNNVL LSLQLLVSML LLVGTALVVS
//