ID   AMPM_MANSE     STANDARD;      PRT;   990 AA.
AC   Q11001;
DT   01-NOV-1997 (REL. 35, CREATED)
DT   01-NOV-1997 (REL. 35, LAST SEQUENCE UPDATE)
DT   01-NOV-1997 (REL. 35, LAST ANNOTATION UPDATE)
DE   MEMBRANE ALANYL AMINOPEPTIDASE PRECURSOR (EC 3.4.11.-)
DE   (AMINOPEPTIDASE N-LIKE PROTEIN) (CRYIA(C) RECEPTOR) (FRAGMENT).
OS   MANDUCA SEXTA (TOBACCO HAWKMOTH) (TOBACCO HORNWORM).
OC   EUKARYOTA; METAZOA; ARTHROPODA; INSECTA; LEPIDOPTERA.
RN   [1]
RP   SEQUENCE FROM N.A., AND PARTIAL SEQUENCE.
RC   TISSUE=MIDGUT;
RX   MEDLINE; 95355365.
RA   KNIGHT P.J.K., KNOWLES B.H., ELLAR D.J.;
RL   J. BIOL. CHEM. 270:17765-17770(1995).
CC   -!- FUNCTION: BINDS TO THE B.THURINGIENSIS TOXIN, CRYIA(C).
CC   -!- COFACTOR: BINDS AND REQUIRES A ZINC ATOM, WHICH IS ESSENTIAL FOR
CC       PROTEOLYTIC ACTIVITY.
CC   -!- SUBCELLULAR LOCATION: ATTACHED TO THE MEMBRANE BY A GPI-ANCHOR.
CC   -!- TISSUE SPECIFICITY: MIDGUT BRUSH-BORDER MEMBRANE.
CC   -!- SIMILARITY: BELONGS TO PEPTIDASE FAMILY M1 (ZINC METALLOPROTEASE);
CC       ALSO KNOWN AS THE PEPN SUBFAMILY.
DR   EMBL; X89081; G953188; -.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
KW   HYDROLASE; METALLOPROTEASE; AMINOPEPTIDASE; ZINC; GLYCOPROTEIN;
KW   GPI-ANCHOR; SIGNAL.
FT   NON_TER       1      1
FT   SIGNAL       <1     15       POTENTIAL.
FT   PROPEP       16     35       ACTIVATION PEPTIDE (BY SIMILARITY).
FT   CHAIN        36    968       MEMBRANE ALANYL AMINOPEPTIDASE.
FT   PROPEP      969    990       REMOVED IN MATURE FORM (POTENTIAL).
FT   METAL       357    357       ZINC (CATALYTIC) (BY SIMILARITY).
FT   ACT_SITE    358    358       BY SIMILARITY.
FT   METAL       361    361       ZINC (CATALYTIC) (BY SIMILARITY).
FT   METAL       380    380       ZINC (CATALYTIC) (BY SIMILARITY).
FT   ACT_SITE    460    460       PROTON DONOR (POTENTIAL).
FT   LIPID       968    968       GPI-ANCHOR (POTENTIAL).
FT   CARBOHYD    295    295       POTENTIAL.
FT   CARBOHYD    609    609       POTENTIAL.
FT   CARBOHYD    623    623       POTENTIAL.
FT   CARBOHYD    752    752       POTENTIAL.
SQ   SEQUENCE   990 AA;  111293 MW;  0EA90F91 CRC32;
     FTIFLGVALL QGVLTLSPIP VPEEEWAEFS RMLRDPSYRL PTTTRPRHYA VTLTPYFDVV
     PAGVSGLTTF SFDGEVTIYI SPTQANVNEI VLHCNDLTIQ SLRVTYVSGN SEVDITATGQ
     TFTCEMPYSF LRIRTSTPLV MNQEYIIRST FRGNLQTNMR GFYRSWYVDR TGKRWMATTQ
     FQPGHARQAF PCYDEPGFKA TFDITMNREA DFSPTISNMP IRATTTLTNG RISETFFTTP
     LTSTYLLAFI VSHYQVISNN NNAARPFRIY ARNNVGSQGD WSLEMGEKLL LAMENYTAIP
     YYTMAQNLDM KQAAIPDFSA GAMENWGLLT YREALILYDP LNSNHHYRQR VANIVSHEIA
     HMWFGNLVTC AWWDNLWLNE GFARFSQYYL TATVDPELGY EIRFIPEQLQ VAMFSDSVDS
     AHALTDTSVN DPVAVSAHFS TITYARGAAI LRMTQHLLSY DTFVKGLRQY LRARQFDVAE
     PYHLFSALDA AAAEDNALAA YRGITIDAYF RTWSEKAGHP LLSVTVDHES GRMTLVQARW
     ERNTGVSRFP GLWHIPITWT RAGAPDFENL KPSQVMTGQS LVIDRGTRGQ EWVIFNKQVS
     GFYRVNYDNT TWGLITRALR SANRTVIHEL SRSQIVDDVF QLARSGVMSY QRALNILSYL
     RFEDAYAPWL SAISGFNWVI RRFAHDAANL QTLQNQIIGL SEAVVARLGF TEVSGGTYMT
     DLQRLHVMQF LCNVGHQQCI DAGRQNFLNW RNGSFIPANM RPWVYCTGLR YGSAEDFNYF
     WNRYIVEDLS NEKVVMLEAA GCTRDQASLE KFLNAIVSGN DDVRPQDHSS ALSSAITSND
     VNTMRAFDWL TKNVDQITRT LGSITSPLNT ITSRLLTEAQ MTQVQTWLDA NRNTIGAAYN
     TGVNGIATSR ANLQWSANRM SEFLRFFETG FVDDVPSEAT TVAPPAETTV TPSTFPPTVA
     PATTPAPGSG NIAALSVVSL LVTLAINMVA
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