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GPI Lipid Anchor Project Sequence analysis of GPI-anchored proteins on the proprotein level Prediction of potential GPI-modification sites in proprotein sequences Big-PI predictor Occurence of potentially GPI modified proteins in complete genomes Prediction of GPI lipid anchors in plants Big-PI Plant Predictor Prediction of GPI lipid anchors in fungi Big-PI Fungal Predictor

Comparison: Application of the big-PI fungal predictor on the N.crassa protein list created by de Groot et al., Yeast. 2003 Jul 15;20(9):781-96

Contact:
Birgit Eisenhaber (IMP/Austria)
Georg Schneider (IMP/Austria)
Michael Wildpaner (IMP/Austria)
Frank Eisenhaber (IMP/Austria)

Summary Table:

	Prediction Quality	Rate	Percentage
	1:P	67/97	69.07%
	1:S	1/97	1.03%
	1:S (Positive Total Score)	-	-
	1:S (Negative Total Score)	1/97	1.03%
	1:I	24/97	24.74%
	1:N	5/97	5.16%
	Not Predicted	30/97	30.93%
	Predicted	67/97	69.07%

Reasons for not predicted proteins are detailed below

N		ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
1		NCU00086.1	369	1:P	352	10.75	-0.12	10.63	3.248148e-05
2		NCU00175.1	286	1:P	261	13.55	-2.49	11.06	2.784096e-05
3		NCU00267.1	198	1:P	171	15.08	-0.86	14.22	8.388785e-06
4		NCU00270.1	401	1:P	372	14.63	-1.14	13.49	1.121015e-05
5		NCU00449.1	261	1:I	244	-11.48	-20.16	-31.64	8.208416e-02
6		NCU00473.1	396	1:P	370	19.32	-0.35	18.97	1.055141e-06
7		NCU00881.1	229	1:P	207	12.58	-0.85	11.73	2.181332e-05
8		NCU01050.1	238	1:I	210	-2.49	-35.29	-37.78	1.303320e-01
9		NCU01162.1	457	1:P	426	11.05	-0.94	10.11	3.894009e-05
10		NCU01351.1	369	1:P	345	11.19	-4.75	6.44	1.295507e-04
11		NCU01353.1	725	1:P	699	16.59	-0.08	16.51	3.213992e-06
12		NCU01403.1	450	1:P	427	11.58	-1.30	10.28	3.675503e-05
13		NCU01418.1	142	1:P	118	17.51	-2.62	14.89	6.378605e-06
14		NCU01462.1	306	1:P	285	16.02	-0.78	15.24	5.530722e-06
15		NCU02041.1	195	1:P	173	20.14	-2.81	17.33	2.239560e-06
16		NCU02170.1	725	1:I	696	0.42	-9.93	-9.51	5.443236e-03
17		NCU02184.1	1434	1:P	1412	15.51	-0.30	15.21	5.587391e-06
18		NCU02880.1	166	1:P	145	16.79	-0.09	16.70	2.967720e-06
19		NCU02884.1	220	1:P	193	14.97	-6.65	8.32	7.148482e-05
20		NCU02900.1	107	1:I	91	-2.94	-17.30	-20.24	2.612408e-02

N		ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
21		NCU02909.1	574	1:P	549	11.03	-4.33	6.70	1.196175e-04
22		NCU02956.1	492	1:P	460	15.84	-1.18	14.66	7.013336e-06
23		NCU03013.1	248	1:P	224	15.57	-2.14	13.43	1.145067e-05
24		NCU03168.1	529	1:P	497	13.89	-0.94	12.95	1.378806e-05
25		NCU03210.1	379	1:P	352	16.92	-0.13	16.79	2.852010e-06
26		NCU03222.1	200	1:P	174	16.81	-4.94	11.87	2.073893e-05
27		NCU03293.1	173	1:P	150	18.28	-1.43	16.85	2.769517e-06
28		NCU03530.1	260	1:P	236	16.40	-0.39	16.01	3.990653e-06
29		NCU03602.1	458	1:P	432	13.45	-2.31	11.14	2.710634e-05
30		NCU03770.1	489	1:I	468	7.14	-13.35	-6.21	2.956474e-03
31		NCU03873.1	178	1:P	154	19.19	-4.43	14.76	6.733081e-06
32		NCU03895.1	132	1:N	112	5.33	-15.97	-10.64	6.611595e-03
33		NCU04395.1	480	1:I	462	-5.46	-12.07	-17.53	1.854751e-02
34		NCU04493.1	172	1:P	140	18.53	-1.06	17.47	2.106155e-06
35		NCU04496.1	221	1:P	200	20.07	-2.29	17.78	1.829506e-06
36		NCU04542.1	224	1:P	200	15.69	-2.30	13.39	1.165550e-05
37		NCU04543.1	310	1:P	286	14.61	-4.15	10.46	3.445253e-05
38		NCU04616.1	624	1:P	597	14.25	-8.41	5.84	1.552519e-04
39		NCU05071.1	351	1:I	329	-0.49	-29.80	-30.29	7.324298e-02
40		NCU05096.1	429	1:I	398	12.55	-19.82	-7.27	3.624284e-03

N		ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
41		NCU05104.1	450	1:N	418	-7.42	-19.14	-26.56	5.195335e-02
42		NCU05229.1	277	1:P	255	16.58	0.00	16.58	3.115881e-06
43		NCU05318.1	275	1:P	251	15.15	-4.22	10.93	2.919838e-05
44		NCU05395.1	122	1:P	97	19.46	-4.85	14.61	7.154122e-06
45		NCU05667.1	219	1:P	197	17.39	-0.01	17.38	2.197038e-06
46		NCU05686.1	477	1:P	445	9.58	-0.69	8.89	5.908071e-05
47		NCU05974.1	364	1:P	341	16.46	-0.24	16.22	3.649016e-06
48		NCU06055.1	433	1:I	413	-7.30	-35.27	-42.57	1.766565e-01
49		NCU06109.1	1376	1:S	1348	5.49	-5.84	-0.35	8.227428e-04
50		NCU06185.1	245	1:P	222	17.65	0.00	17.65	1.946312e-06
51		NCU06381.1	406	1:P	379	15.86	-2.89	12.97	1.368357e-05
52		NCU06607.1	572	1:P	552	14.10	-1.54	12.56	1.601299e-05
53		NCU06703.1	699	1:P	675	14.63	-0.06	14.57	7.271053e-06
54		NCU06781.1	494	1:P	468	18.36	-1.07	17.29	2.280877e-06
55		NCU07050.1	828	1:N	811	1.20	-9.63	-8.43	4.490851e-03
56		NCU07063.1	551	1:P	525	13.28	-1.89	11.39	2.473326e-05
57		NCU07159.1	396	1:I	369	-1.83	-49.41	-51.24	2.770063e-01
58		NCU07253.1	466	1:P	435	13.33	-0.21	13.12	1.290373e-05
59		NCU07277.1	734	1:P	713	16.84	0.00	16.84	2.787803e-06
60		NCU07355.1	464	1:I	443	1.10	-39.76	-38.66	1.383179e-01

N		ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
61		NCU07454.1	186	1:P	165	17.86	0.00	17.86	1.764273e-06
62		NCU07486.1	265	1:I	249	0.20	-25.87	-25.67	4.758317e-02
63		NCU07533.1	603	1:I	583	-1.05	-44.28	-45.33	2.064770e-01
64		NCU07776.1	190	1:P	169	17.19	-0.49	16.70	2.966990e-06
65		NCU07881.1	302	1:I	268	6.52	-15.60	-9.08	5.049890e-03
66		NCU07962.1	183	1:P	159	16.24	-2.05	14.19	8.464899e-06
67		NCU08090.1	122	1:P	96	16.31	-1.66	14.65	7.050129e-06
68		NCU08131.1	533	1:P	509	18.35	-0.56	17.79	1.826123e-06
69		NCU08171.1	381	1:I	365	0.59	-23.69	-23.10	3.632710e-02
70		NCU08193.1	229	1:P	204	17.20	-4.04	13.16	1.274155e-05
71		NCU08227.1	321	1:N	296	0.06	-5.90	-5.84	2.743519e-03
72		NCU08318.1	246	1:P	222	16.08	-1.70	14.38	7.860479e-06
73		NCU08457.1	108	1:I	81	-5.92	-22.57	-28.49	6.236210e-02
74		NCU08523.1	326	1:I	294	10.31	-23.45	-13.14	9.890240e-03
75		NCU08680.1	137	1:I	123	7.43	-39.66	-32.23	8.616607e-02
76		NCU08909.1	542	1:N	524	-3.75	-6.67	-10.42	6.373998e-03
77		NCU09055.1	89	1:I	61	0.57	-13.05	-12.48	8.923317e-03
78		NCU09072.1	329	1:P	298	19.12	-1.70	17.42	2.157165e-06
79		NCU09099.1	283	1:P	259	17.98	-0.52	17.46	2.115141e-06
80		NCU09102.1	604	1:I	579	-3.76	-20.00	-23.76	3.901510e-02

N		ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
81		NCU09117.1	375	1:P	346	13.10	-1.11	11.99	1.980280e-05
82		NCU09133.1	257	1:P	234	19.42	-1.41	18.01	1.649909e-06
83		NCU09155.1	518	1:P	492	15.40	-0.55	14.85	6.483579e-06
84		NCU09175.1	410	1:P	384	17.92	-1.26	16.66	3.018579e-06
85		NCU09225.1	360	1:I	337	1.48	-23.11	-21.63	3.077550e-02
86		NCU09263.1	207	1:P	182	16.62	-0.57	16.05	3.927921e-06
87		NCU09525.1	206	1:I	177	-12.83	-41.88	-54.71	3.219854e-01
88		NCU09568.1	342	1:P	317	17.95	-0.69	17.26	2.316054e-06
89		NCU09647.1	326	1:P	301	13.26	-0.18	13.08	1.312963e-05
90		NCU09672.1	320	1:I	301	5.01	-65.82	-60.81	4.050765e-01
91		NCU09729.1	886	1:P	859	9.40	-3.17	6.23	1.378140e-04
92		NCU09733.1	476	1:P	453	16.14	0.00	16.14	3.772453e-06
93		NCU09775.1	342	1:I	323	-9.70	-17.70	-27.40	5.635329e-02
94		NCU09785.1	516	1:P	486	11.67	-8.02	3.65	2.924293e-04
95		NCU09851.1	252	1:P	225	19.33	-1.62	17.71	1.890291e-06
96		NCU09868.1	131	1:P	103	18.97	-0.12	18.85	1.117670e-06
97		NCU09929.1	281	1:P	260	13.20	-0.09	13.11	1.299609e-05

30 proteins described in the paper have not been predicted as suitable candidates for GPI anchoring:

1. 2 proteins have an untypical N-terminal region [-11..-1] (relative to the omega-site):

NCU06109.1 (S<0)
NCU08227.1


2. We could not detect a suitable omega-site for 7 proteins:

NCU00449.1
NCU02170.1
NCU02900.1
NCU04395.1
NCU07050.1
NCU07881.1
NCU09225.1


3. In 1 further protein a typical spacer region is missing:

NCU03770.1


4. 12 proteins lack an appropriately constructed hydrophobic tail:

NCU01050.1
NCU05104.1
NCU03895.1
NCU05071.1
NCU05096.1
NCU07159.1
NCU07355.1
NCU07533.1
NCU08457.1
NCU08523.1
NCU09102.1
NCU09672.1


5. 8 sequences are non-compatible with our prediction function at all:

NCU06055.1
NCU07486.1
NCU08171.1
NCU08680.1
NCU08909.1
NCU09055.1
NCU09525.1
NCU09775.1

Last modified: 24th September 2003