Comparison: Application of the big-PI fungal predictor on the C.albicans protein list created by de Groot et al., Yeast. 2003 Jul 15;20(9):781-96

Contact:
Birgit Eisenhaber (IMP/Austria)
Georg Schneider (IMP/Austria)
Michael Wildpaner (IMP/Austria)
Frank Eisenhaber (IMP/Austria)

Summary Table:

Prediction Quality	Rate	Percentage
1:P	78/104	75.00%
1:S	7/104	6.73%
1:S (Positive Total Score)	4/104	3.85%
1:S (Negative Total Score)	3/104	2.88%
1:I	13/104	12.50%
1:N	6/104	5.77%
Not Predicted	22/104	21.15%
Predicted	82/104	78.85%

Reasons for not predicted proteins are detailed below

N ID Length Prediction Quality Site Profile Score Function Score Total Score PValue

1 orf19.5741.prot 1260 1:P 1239 13.38 -0.29 13.09 1.306531e-05

2 orf19.2355.prot 1586 1:P 1565 11.91 -1.60 10.31 3.638391e-05

3 orf19.5745.prot 763 1:I 742 14.23 -14.81 -0.58 8.695993e-04

4 orf19.2122.prot 158 1:P 137 14.59 -1.26 13.33 1.189558e-05

5 orf19.1816.prot 882 1:I 867 -6.87 -80.28 -87.15 7.437345e-01

6 orf19.4556.prot 537 1:P 516 14.59 -1.26 13.33 1.189558e-05

7 orf19.7414.prot 1366 1:P 1345 15.97 -1.41 14.56 7.307818e-06

8 orf19.7414.prot 1366 1:P 1345 15.97 -1.41 14.56 7.307818e-06

9 orf19.7400.prot 2000 1:I 1982 6.80 -21.86 -15.06 1.318031e-02

10 orf19.5742.prot 894 1:I 878 0.05 -41.73 -41.68 1.675184e-01

11 orf19.7517.prot 462 1:N 439 -4.84 -6.49 -11.33 7.415731e-03

12 orf19.3895.prot 583 1:P 560 14.40 -1.96 12.44 1.672822e-05

13 orf19.2706.prot 453 1:P 430 18.36 -0.42 17.94 1.705646e-06

14 orf19.7114.prot 1018 1:S 989 7.83 -6.19 1.64 5.008974e-04

15 orf19.2075.prot 451 1:P 429 12.13 -9.72 2.41 4.087767e-04

16 CaDB 5366.2 (a) 42 1:P 19 19.25 -0.21 19.04 1.021461e-06

17 orf19.4255.prot 413 1:P 384 18.00 -0.09 17.91 1.722975e-06

18 orf19.2952.prot 479 1:P 456 17.70 -0.12 17.58 2.007684e-06

19 orf19.1321.prot 634 1:P 613 15.99 -0.91 15.08 5.909494e-06

20 orf19.4975.prot 919 1:P 895 17.52 -2.37 15.15 5.735837e-06

N ID Length Prediction Quality Site Profile Score Function Score Total Score PValue

21 orf19.575.prot 1249 1:P 1225 17.67 -4.00 13.67 1.044426e-05

22 orf19.4361.prot 941 1:P 917 17.29 -3.38 13.91 9.466503e-06

23 orf19.7472.prot 1526 1:P 1502 16.31 -0.58 15.73 4.488124e-06

24 orf19.2879.prot 1308 1:P 1284 14.56 -3.50 11.06 2.789530e-05

25 orf19.4072.prot 1086 1:P 1063 13.85 -2.15 11.70 2.204847e-05

26 orf19.3279.prot 1225 1:S 1195 5.13 -4.23 0.90 6.050745e-04

27 orf19.570.prot 714 1:I 690 16.13 -19.13 -3.00 1.516702e-03

28 orf19.4377.prot 130 1:I 103 4.78 -8.09 -3.31 1.622095e-03

29 orf19.3212.prot 558 1:P 536 13.41 -8.34 5.07 1.949874e-04

30 orf19.7625.prot 132 1:P 100 7.80 -4.85 2.95 3.535450e-04

31 orf19.2062.prot 232 1:P 209 19.68 0.00 19.68 7.508915e-07

32 orf19.2060.prot 228 1:P 205 18.45 -0.17 18.28 1.458737e-06

33 orf19.4035.prot 451 1:P 430 13.38 -1.01 12.37 1.718899e-05

34 orf19.3693.prot 641 1:N 614 4.35 -14.72 -10.37 6.318712e-03

35 orf19.4765.prot 219 1:P 194 17.54 -0.56 16.98 2.625144e-06

36 orf19.5635.prot 219 1:P 194 20.03 -2.46 17.57 2.014214e-06

37 orf19.3380.prot 908 1:P 887 13.32 -1.00 12.32 1.755642e-05

38 orf19.2108.prot 316 1:S 288 2.31 -3.19 -0.88 9.355413e-04

39 orf19.5674.prot 250 1:P 230 15.04 -1.32 13.72 1.022385e-05

40 orf19.7609.prot 124 1:P 107 14.92 -0.92 14.00 9.154150e-06

N ID Length Prediction Quality Site Profile Score Function Score Total Score PValue

41 orf19.7597.prot 315 1:I 286 -17.40 -45.95 -63.35 4.406206e-01

42 orf19.6420.prot 456 1:P 433 14.91 -5.25 9.66 4.562703e-05

43 orf19.968.prot 131 1:P 105 19.06 0.00 19.06 1.011760e-06

44 orf19.2878.prot 165 1:P 143 16.92 -0.18 16.74 2.907486e-06

45 orf19.848.prot 107 1:N 79 3.20 -9.74 -6.54 3.151594e-03

46 orf19.893.prot 557 1:P 533 15.63 -3.86 11.77 2.149332e-05

47 orf19.301.prot 753 1:P 730 18.63 -0.07 18.56 1.279576e-06

48 orf19.2033.prot 229 1:N 199 -1.93 -6.80 -8.73 4.745641e-03

49 orf19.535.prot 111 1:P 81 19.53 -0.06 19.47 8.305538e-07

50 orf19.532.prot 168 1:P 143 18.09 -0.34 17.75 1.857078e-06

51 orf19.3738.prot 169 1:P 140 10.18 -2.34 7.84 8.342205e-05

52 orf19.3740.prot 282 1:P 259 18.01 -0.10 17.91 1.728803e-06

53 orf19.3618.prot 533 1:P 511 15.06 -1.04 14.02 9.070544e-06

54 orf19.6336.prot 872 1:S 845 7.73 -9.53 -1.80 1.157122e-03

55 orf19.2475.prot 131 1:P 110 15.32 -1.46 13.86 9.687499e-06

56 orf19.2044.prot 485 1:I 458 4.58 -12.71 -8.13 4.250408e-03

57 orf19.5144.prot 226 1:P 197 20.93 -0.03 20.90 4.110888e-07

58 orf19.5305.prot 204 1:P 182 17.05 -0.40 16.65 3.023207e-06

59 orf19.5303.prot 277 1:P 253 17.56 -0.22 17.34 2.236899e-06

60 orf19.5302.prot 352 1:P 330 17.58 -1.15 16.43 3.331582e-06

N ID Length Prediction Quality Site Profile Score Function Score Total Score PValue

61 orf19.6784.prot 430 1:N 408 2.39 -7.15 -4.76 2.206675e-03

62 orf19.876.prot 246 1:P 219 16.80 -1.20 15.60 4.751322e-06

63 orf19.2833.prot 197 1:P 169 17.14 -0.19 16.95 2.656857e-06

64 orf19.4910.prot 250 1:N 231 -9.02 -23.22 -32.24 8.624770e-02

65 orf19.5760.prot 392 1:P 363 16.86 -0.05 16.81 2.825872e-06

66 orf19.3923.prot 208 1:P 185 16.42 -0.99 15.43 5.101441e-06

67 orf19.2759.prot 220 1:P 195 12.56 -1.76 10.80 3.055477e-05

68 orf19.6302.prot 281 1:P 256 12.90 -2.16 10.74 3.121195e-05

69 orf19.1616.prot 1114 1:S 1089 5.93 -6.74 -0.81 9.183240e-04

70 orf19.2906.prot 296 1:P 275 14.52 -0.54 13.98 9.203701e-06

71 orf19.2907.prot 227 1:P 200 12.15 -8.35 3.80 2.802060e-04

72 orf19.2910.prot 236 1:I 205 5.44 -11.02 -5.58 2.608389e-03

73 orf19.1714.prot 307 1:P 286 13.14 -0.63 12.51 1.629468e-05

74 orf19.2451.prot 462 1:P 440 12.56 -2.70 9.86 4.253276e-05

75 orf19.3638.prot 340 1:P 314 15.15 -9.31 5.84 1.553085e-04

76 orf19.1401.prot 653 1:P 632 13.61 -0.92 12.69 1.522636e-05

77 orf19.6321.prot 108 1:P 84 19.26 -2.50 16.76 2.880508e-06

78 orf19.4404.prot 734 1:P 707 8.66 -2.10 6.56 1.245805e-04

79 orf19.1824.prot 311 1:P 288 12.27 -1.78 10.49 3.414476e-05

80 orf19.1989.prot 452 1:I 430 10.40 -18.04 -7.64 3.888688e-03

N ID Length Prediction Quality Site Profile Score Function Score Total Score PValue

81 orf19.1911.prot 384 1:P 361 14.01 -0.03 13.98 9.210123e-06

82 orf19.4651.prot 139 1:P 116 16.56 -0.37 16.19 3.697507e-06

83 orf19.2685.prot 342 1:I 314 -0.63 -44.79 -45.42 2.074960e-01

84 orf19.207.prot 1404 1:P 1382 15.93 -3.29 12.64 1.555914e-05

85 CaDB 15957.2 (a) 42 1:P 20 19.35 -0.33 19.02 1.028683e-06

86 orf19.4689.prot 214 1:S 191 5.96 -4.92 1.04 5.842552e-04

87 orf19.4334.prot 240 1:P 219 16.63 -0.67 15.96 4.072296e-06

88 orf19.2767.prot 113 1:P 92 17.04 -0.26 16.78 2.856666e-06

89 orf19.5588.prot 765 1:P 743 14.14 -3.97 10.17 3.820505e-05

90 orf19.5762.prot 223 1:P 202 14.88 -3.30 11.58 2.304027e-05

91 orf19.2765.prot 213 1:P 194 16.10 -1.13 14.97 6.163411e-06

92 orf19.6216.prot 148 1:I 117 -7.93 -26.26 -34.19 1.005634e-01

93 orf19.3829.prot 548 1:P 516 16.84 -2.31 14.53 7.406665e-06

94 orf19.6081.prot 544 1:P 515 18.17 -2.83 15.34 5.307519e-06

95 orf19.6594.prot 632 1:P 607 14.02 -4.56 9.46 4.882412e-05

96 orf19.1443.prot 304 1:P 280 9.07 -4.25 4.82 2.097886e-04

97 orf19.5102.prot 754 1:P 726 11.54 -1.61 9.93 4.154567e-05

98 orf19.1327.prot 714 1:P 693 15.99 -0.91 15.08 5.909494e-06

99 orf19.5636.prot 241 1:P 221 15.19 -2.09 13.10 1.301495e-05

100 orf19.6928.prot 544 1:P 520 13.80 -0.12 13.68 1.038736e-05

N ID Length Prediction Quality Site Profile Score Function Score Total Score PValue

101 orf19.2237.prot 525 1:S 502 7.98 -6.18 1.80 4.809279e-04

102 orf19.7030.prot 234 1:P 215 15.34 -3.96 11.38 2.482256e-05

103 orf19.1671.prot 470 1:P 440 12.18 -1.94 10.24 3.725081e-05

104 orf19.1127.prot 105 1:I 87 -7.61 -71.77 -79.38 6.562314e-01

(a) only C-terminal 42 residues

N	ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
1	orf19.5741.prot	1260	1:P	1239	13.38	-0.29	13.09	1.306531e-05
2	orf19.2355.prot	1586	1:P	1565	11.91	-1.60	10.31	3.638391e-05
3	orf19.5745.prot	763	1:I	742	14.23	-14.81	-0.58	8.695993e-04
4	orf19.2122.prot	158	1:P	137	14.59	-1.26	13.33	1.189558e-05
5	orf19.1816.prot	882	1:I	867	-6.87	-80.28	-87.15	7.437345e-01
6	orf19.4556.prot	537	1:P	516	14.59	-1.26	13.33	1.189558e-05
7	orf19.7414.prot	1366	1:P	1345	15.97	-1.41	14.56	7.307818e-06
8	orf19.7414.prot	1366	1:P	1345	15.97	-1.41	14.56	7.307818e-06
9	orf19.7400.prot	2000	1:I	1982	6.80	-21.86	-15.06	1.318031e-02
10	orf19.5742.prot	894	1:I	878	0.05	-41.73	-41.68	1.675184e-01
11	orf19.7517.prot	462	1:N	439	-4.84	-6.49	-11.33	7.415731e-03
12	orf19.3895.prot	583	1:P	560	14.40	-1.96	12.44	1.672822e-05
13	orf19.2706.prot	453	1:P	430	18.36	-0.42	17.94	1.705646e-06
14	orf19.7114.prot	1018	1:S	989	7.83	-6.19	1.64	5.008974e-04
15	orf19.2075.prot	451	1:P	429	12.13	-9.72	2.41	4.087767e-04
16	CaDB 5366.2 (a)	42	1:P	19	19.25	-0.21	19.04	1.021461e-06
17	orf19.4255.prot	413	1:P	384	18.00	-0.09	17.91	1.722975e-06
18	orf19.2952.prot	479	1:P	456	17.70	-0.12	17.58	2.007684e-06
19	orf19.1321.prot	634	1:P	613	15.99	-0.91	15.08	5.909494e-06
20	orf19.4975.prot	919	1:P	895	17.52	-2.37	15.15	5.735837e-06

N	ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
21	orf19.575.prot	1249	1:P	1225	17.67	-4.00	13.67	1.044426e-05
22	orf19.4361.prot	941	1:P	917	17.29	-3.38	13.91	9.466503e-06
23	orf19.7472.prot	1526	1:P	1502	16.31	-0.58	15.73	4.488124e-06
24	orf19.2879.prot	1308	1:P	1284	14.56	-3.50	11.06	2.789530e-05
25	orf19.4072.prot	1086	1:P	1063	13.85	-2.15	11.70	2.204847e-05
26	orf19.3279.prot	1225	1:S	1195	5.13	-4.23	0.90	6.050745e-04
27	orf19.570.prot	714	1:I	690	16.13	-19.13	-3.00	1.516702e-03
28	orf19.4377.prot	130	1:I	103	4.78	-8.09	-3.31	1.622095e-03
29	orf19.3212.prot	558	1:P	536	13.41	-8.34	5.07	1.949874e-04
30	orf19.7625.prot	132	1:P	100	7.80	-4.85	2.95	3.535450e-04
31	orf19.2062.prot	232	1:P	209	19.68	0.00	19.68	7.508915e-07
32	orf19.2060.prot	228	1:P	205	18.45	-0.17	18.28	1.458737e-06
33	orf19.4035.prot	451	1:P	430	13.38	-1.01	12.37	1.718899e-05
34	orf19.3693.prot	641	1:N	614	4.35	-14.72	-10.37	6.318712e-03
35	orf19.4765.prot	219	1:P	194	17.54	-0.56	16.98	2.625144e-06
36	orf19.5635.prot	219	1:P	194	20.03	-2.46	17.57	2.014214e-06
37	orf19.3380.prot	908	1:P	887	13.32	-1.00	12.32	1.755642e-05
38	orf19.2108.prot	316	1:S	288	2.31	-3.19	-0.88	9.355413e-04
39	orf19.5674.prot	250	1:P	230	15.04	-1.32	13.72	1.022385e-05
40	orf19.7609.prot	124	1:P	107	14.92	-0.92	14.00	9.154150e-06

N	ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
41	orf19.7597.prot	315	1:I	286	-17.40	-45.95	-63.35	4.406206e-01
42	orf19.6420.prot	456	1:P	433	14.91	-5.25	9.66	4.562703e-05
43	orf19.968.prot	131	1:P	105	19.06	0.00	19.06	1.011760e-06
44	orf19.2878.prot	165	1:P	143	16.92	-0.18	16.74	2.907486e-06
45	orf19.848.prot	107	1:N	79	3.20	-9.74	-6.54	3.151594e-03
46	orf19.893.prot	557	1:P	533	15.63	-3.86	11.77	2.149332e-05
47	orf19.301.prot	753	1:P	730	18.63	-0.07	18.56	1.279576e-06
48	orf19.2033.prot	229	1:N	199	-1.93	-6.80	-8.73	4.745641e-03
49	orf19.535.prot	111	1:P	81	19.53	-0.06	19.47	8.305538e-07
50	orf19.532.prot	168	1:P	143	18.09	-0.34	17.75	1.857078e-06
51	orf19.3738.prot	169	1:P	140	10.18	-2.34	7.84	8.342205e-05
52	orf19.3740.prot	282	1:P	259	18.01	-0.10	17.91	1.728803e-06
53	orf19.3618.prot	533	1:P	511	15.06	-1.04	14.02	9.070544e-06
54	orf19.6336.prot	872	1:S	845	7.73	-9.53	-1.80	1.157122e-03
55	orf19.2475.prot	131	1:P	110	15.32	-1.46	13.86	9.687499e-06
56	orf19.2044.prot	485	1:I	458	4.58	-12.71	-8.13	4.250408e-03
57	orf19.5144.prot	226	1:P	197	20.93	-0.03	20.90	4.110888e-07
58	orf19.5305.prot	204	1:P	182	17.05	-0.40	16.65	3.023207e-06
59	orf19.5303.prot	277	1:P	253	17.56	-0.22	17.34	2.236899e-06
60	orf19.5302.prot	352	1:P	330	17.58	-1.15	16.43	3.331582e-06

N	ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
61	orf19.6784.prot	430	1:N	408	2.39	-7.15	-4.76	2.206675e-03
62	orf19.876.prot	246	1:P	219	16.80	-1.20	15.60	4.751322e-06
63	orf19.2833.prot	197	1:P	169	17.14	-0.19	16.95	2.656857e-06
64	orf19.4910.prot	250	1:N	231	-9.02	-23.22	-32.24	8.624770e-02
65	orf19.5760.prot	392	1:P	363	16.86	-0.05	16.81	2.825872e-06
66	orf19.3923.prot	208	1:P	185	16.42	-0.99	15.43	5.101441e-06
67	orf19.2759.prot	220	1:P	195	12.56	-1.76	10.80	3.055477e-05
68	orf19.6302.prot	281	1:P	256	12.90	-2.16	10.74	3.121195e-05
69	orf19.1616.prot	1114	1:S	1089	5.93	-6.74	-0.81	9.183240e-04
70	orf19.2906.prot	296	1:P	275	14.52	-0.54	13.98	9.203701e-06
71	orf19.2907.prot	227	1:P	200	12.15	-8.35	3.80	2.802060e-04
72	orf19.2910.prot	236	1:I	205	5.44	-11.02	-5.58	2.608389e-03
73	orf19.1714.prot	307	1:P	286	13.14	-0.63	12.51	1.629468e-05
74	orf19.2451.prot	462	1:P	440	12.56	-2.70	9.86	4.253276e-05
75	orf19.3638.prot	340	1:P	314	15.15	-9.31	5.84	1.553085e-04
76	orf19.1401.prot	653	1:P	632	13.61	-0.92	12.69	1.522636e-05
77	orf19.6321.prot	108	1:P	84	19.26	-2.50	16.76	2.880508e-06
78	orf19.4404.prot	734	1:P	707	8.66	-2.10	6.56	1.245805e-04
79	orf19.1824.prot	311	1:P	288	12.27	-1.78	10.49	3.414476e-05
80	orf19.1989.prot	452	1:I	430	10.40	-18.04	-7.64	3.888688e-03

N	ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
81	orf19.1911.prot	384	1:P	361	14.01	-0.03	13.98	9.210123e-06
82	orf19.4651.prot	139	1:P	116	16.56	-0.37	16.19	3.697507e-06
83	orf19.2685.prot	342	1:I	314	-0.63	-44.79	-45.42	2.074960e-01
84	orf19.207.prot	1404	1:P	1382	15.93	-3.29	12.64	1.555914e-05
85	CaDB 15957.2 (a)	42	1:P	20	19.35	-0.33	19.02	1.028683e-06
86	orf19.4689.prot	214	1:S	191	5.96	-4.92	1.04	5.842552e-04
87	orf19.4334.prot	240	1:P	219	16.63	-0.67	15.96	4.072296e-06
88	orf19.2767.prot	113	1:P	92	17.04	-0.26	16.78	2.856666e-06
89	orf19.5588.prot	765	1:P	743	14.14	-3.97	10.17	3.820505e-05
90	orf19.5762.prot	223	1:P	202	14.88	-3.30	11.58	2.304027e-05
91	orf19.2765.prot	213	1:P	194	16.10	-1.13	14.97	6.163411e-06
92	orf19.6216.prot	148	1:I	117	-7.93	-26.26	-34.19	1.005634e-01
93	orf19.3829.prot	548	1:P	516	16.84	-2.31	14.53	7.406665e-06
94	orf19.6081.prot	544	1:P	515	18.17	-2.83	15.34	5.307519e-06
95	orf19.6594.prot	632	1:P	607	14.02	-4.56	9.46	4.882412e-05
96	orf19.1443.prot	304	1:P	280	9.07	-4.25	4.82	2.097886e-04
97	orf19.5102.prot	754	1:P	726	11.54	-1.61	9.93	4.154567e-05
98	orf19.1327.prot	714	1:P	693	15.99	-0.91	15.08	5.909494e-06
99	orf19.5636.prot	241	1:P	221	15.19	-2.09	13.10	1.301495e-05
100	orf19.6928.prot	544	1:P	520	13.80	-0.12	13.68	1.038736e-05

N	ID	Length	Prediction Quality	Site	Profile Score	Function Score	Total Score	PValue
101	orf19.2237.prot	525	1:S	502	7.98	-6.18	1.80	4.809279e-04
102	orf19.7030.prot	234	1:P	215	15.34	-3.96	11.38	2.482256e-05
103	orf19.1671.prot	470	1:P	440	12.18	-1.94	10.24	3.725081e-05
104	orf19.1127.prot	105	1:I	87	-7.61	-71.77	-79.38	6.562314e-01

22 proteins described in the paper have not been predicted as suitable candidates for GPI anchoring:

We could not detect a suitable omega-site for 7 proteins:

In further 2 proteins a typical spacer region is missing:

8 proteins lack an appropriately constructed hydrophobic tail:

5 sequences are non-compatible with our prediction function at all:

Comparison: Application of the big-PI fungal predictor on the C.albicans protein list created by de Groot et al., Yeast. 2003 Jul 15;20(9):781-96

Contact: Birgit Eisenhaber (IMP/Austria) Georg Schneider (IMP/Austria) Michael Wildpaner (IMP/Austria) Frank Eisenhaber (IMP/Austria)

Summary Table:

Last modified: 23rd September 2003

Contact:
Birgit Eisenhaber (IMP/Austria)
Georg Schneider (IMP/Austria)
Michael Wildpaner (IMP/Austria)
Frank Eisenhaber (IMP/Austria)