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Fungi: Prediction function parametrization

Contact:
Birgit Eisenhaber (IMP/Austria)
Georg Schneider (IMP/Austria)
Michael Wildpaner (IMP/Austria)
Frank Eisenhaber (IMP/Austria)

# 
# ------------------------------------------------------------------- #
# 
# package *-> main.Linux_i686 <-*
# *******************************
# 
# copyright by Birgit Eisenhaber, September 1997
#              E-mail Birgit.Eisenhaber@imp.univie.ac.at
#              WWW    http://mendel.imp.univie.ac.at
#              Post   IMP, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria
#              Tel.   +43-1-79730557, FAX +43-1-7987153
# All rights reserved.
# 
# name of executable           : main.Linux_i686
# time of program compilation  : Jun 17 2003 (16:16:40)
# time of program execution    : Tue Jun 17 16:50:24 2003
# version of the code          : Revision: 4.1 (Date: 2003/05/20 14:22:12)
#
ReadAapLib: AAProperty library opened
Number of entries in AapLib: <682>
-->All selects were switched on.


Selection parameters:
---------------------
0:     PToken: OC   
       SToken: 
         RExp: FUNGI
    is_SToken: 0
       expect: 1

-->begin GPI-site evaluations
-->ReadGPILib: GPI library opened
number of entries of file 1                   :   254
number of accepted entries of file 1          :   254
total number of entries                       :   254

-->Statistic_confidence of GPILib 1 : 
number of accepted entries                    :   254
number of entries with certain GPI-site       :     5
number of entries with potential GPI-site     :   249
number of entries with GPI-site by similarity :     0

-->GPIStatistic_Length: GPILibNumber = 1
Lmin                :  17 ( entry: 7609, file: 1 )
Lmax                :  33 ( entry: AAC35942, file: 1 )
deltaL              :   5
number of intervals :   4
interval of length   from     to    number of entries
                 1     17     22                   72
                 2     23     27                  133
                 3     28     32                   48
                 4     33     37                    1
total number of entries                       :   254

-->Statistic_taxonomy of GPILib 1 / total number of entries 254
   254 ..Fungi

-->degree of the polynom for fitting: 6
-->Calc_EVD_Param_PolyFit: file of values  opened
SSerr =       10.56744212
The 7 polynomial coefficients (starting from the lowest power on) are:
beta[0] = -7.186749e+00
beta[1] = -2.443516e-01
beta[2] = -4.286925e-03
beta[3] = -5.082074e-05
beta[4] = -3.594888e-07
beta[5] = -1.340176e-09
beta[6] = -2.006659e-12

Thresholds for GPI-anchor selecting:
   P-site: score =   2.00  p = 4.558987e-04
           score =   0.00  p = 7.562588e-04
   S-site: score =  -2.00  p = 1.212103e-03
-->All flags were switched on.

-->Switch_Off_Tax: number of entries before procedure : 254
-->Flags of all entries except Fungi switched off
-->Switch_Off_Tax: number of entries after procedure : 254

-->Switch_Off_Len: number of entries before procedure : 254
-->Flags of all entries with AALen-GPIPos > 32 switched off
-->Flags of all entries with AALen-GPIPos < 17 switched off
-->Switch_Off_Len: number of entries after procedure : 253

possible largest subset found: Q =  233
possible largest subset found: Q =  227
possible largest subset found: Q =  222
possible largest subset found: Q =  216
possible largest subset found: Q =  206
possible largest subset found: Q =  192
largest subset found: q = 192
number of 0's in found largest subset: 0
-->Mean and Dispersion of property NAKH920105 from -11 to -1
   Mean: 5.441908
   Dispersion: 1.527800
-->Mean and Dispersion of property NAKH920105 from 3 to 8
   Mean: 6.905547
   Dispersion: 2.376567
-->Mean and Dispersion of property NAKH920105 from 10 to Sequence C-end
   Mean: 12.001243
   Dispersion: 1.720968
-->Mean and Dispersion of property NAKH920105 from 26 to Sequence C-end
   Mean: 12.630885
   Dispersion: 2.396485
-->Mean and Dispersion of property HAGECH94_V from 3 to 8
   Mean: 129.815104
   Dispersion: 16.220161
-->Mean and Dispersion of property  at position(s) -1 0 1 2 
   number of selected entries: 192
   Mean : 386.603646
   Dispersion:  44.154918
-->Mean and Dispersion of property  at position(s) 0 1 2 
   number of selected entries: 192
   Mean : 273.077604
   Dispersion:  32.784327
-->Mean and Dispersion of property  at position(s) 0 1 
   number of selected entries: 192
   Mean : 185.074479
   Dispersion:  31.224105
-->Mean and Dispersion of property  at position(s) 1 2 
   number of selected entries: 192
   Mean : 178.174479
   Dispersion:  26.730564
-->All flags were switched on.

-->Switch_Off_Tax: number of entries before procedure : 254
-->Flags of all entries except Fungi switched off
-->Switch_Off_Tax: number of entries after procedure : 254

-->Switch_Off_Len: number of entries before procedure : 254
-->Flags of all entries with AALen-GPIPos > 32 switched off
-->Flags of all entries with AALen-GPIPos < 17 switched off
-->Switch_Off_Len: number of entries after procedure : 253

NSubFam = 99
Profile:
Ala
  0.4   0.4   0.1   0.2   0.4   0.2   0.2   0.2   0.4   0.1  -0.1  -0.0   0.0  -0.0   0.4 
  0.4   1.5   1.9   0.2   0.4   0.5   0.5   0.3   0.4   0.2   0.7   0.6   0.2   0.7   0.9 
  0.7   0.9   0.6   1.0   1.1   1.0   0.6   0.8   0.9   0.5   0.7 
Cys
 -0.3  -1.4  -2.6  -0.7  -0.0  -4.2  -4.0  -3.2  -2.6   0.2  -0.7  -3.2  -3.2  -3.2  -3.8 
 -0.7  -5.1  -1.4  -3.2  -0.8  -2.7  -1.4  -0.7  -0.7  -0.7  -1.4  -0.7  -0.8  -1.4  -0.3 
  0.5  -0.7  -1.4  -5.6  -5.6  -0.7  -1.4  -1.2  -0.4  -0.9  -0.7 
Asp
 -0.5   0.0  -0.4  -0.4  -0.5  -0.4  -1.4  -0.8  -0.3  -0.7  -0.5  -0.5  -0.5  -0.1  -0.3 
 -1.0  -5.1  -2.4  -1.2  -1.1  -1.1  -1.4  -1.4  -1.4  -1.4  -2.5  -4.6  -4.5  -2.5  -5.2 
 -4.2  -5.2  -5.4  -5.6  -5.6  -5.4  -5.5  -5.1  -2.2  -4.9  -4.7 
Glu
 -0.7  -0.5  -0.7  -0.7  -0.7  -0.8  -1.2  -0.5  -0.7  -0.2  -0.3  -0.3  -0.3   0.0   0.6 
 -6.2  -1.9  -5.9  -1.6  -1.1  -0.9  -0.5  -1.5  -1.3  -1.3  -2.0  -1.9  -2.7  -3.8  -5.2 
 -2.6  -1.9  -5.4  -5.6  -5.6  -5.4  -5.5  -2.5  -4.9  -4.9  -4.7 
Phe
 -0.8  -1.5  -0.8  -1.1  -2.2  -4.2  -4.0  -2.2  -1.1  -3.4  -1.1  -1.5   0.2   0.0  -2.2 
 -6.2  -5.1  -5.9  -0.8  -0.6   0.0  -0.2   0.2  -0.1   0.7   0.6   0.3   0.7   0.7   0.4 
  0.7   0.7   0.5   1.0   0.7   0.7   1.2   1.0   1.3   1.3   0.3 
Gly
  0.0   0.1   0.5   0.3   0.5   0.3   0.5  -0.1   0.3   0.1   0.2   0.1  -0.4  -0.5   0.4 
  1.2   1.3   1.1   0.4  -0.1  -0.2   0.4   0.2   0.6   0.6   0.4   0.6   0.5   0.3   0.3 
  0.8   0.6   0.4   0.4   0.1  -0.0  -0.0  -0.0   0.2  -0.5   0.6 
His
 -0.0  -0.2  -0.5  -0.5  -1.6  -4.2  -0.9  -1.6  -0.2  -0.5  -3.3  -0.9  -0.0   0.1  -0.9 
 -6.2  -5.1  -5.9  -0.5   0.6  -0.9  -0.0  -0.9  -1.6  -0.0  -3.9  -4.6  -4.5  -1.6  -5.2 
 -4.2  -5.2  -5.4  -5.6  -1.6  -1.6  -5.5  -0.7  -4.9  -0.4  -4.7 
Ile
 -1.2  -0.3  -0.5  -1.1  -1.1  -0.7  -0.7  -0.3  -0.1  -0.0   0.0  -0.5  -0.2  -0.6  -1.5 
 -6.2  -5.1  -5.9  -0.1   0.2   0.3  -0.5   0.1  -0.2  -0.2   0.3  -0.0   0.2   0.7   0.3 
  0.4   0.5   0.6   0.6   0.5   0.3   0.7   0.4   0.7   0.6   0.6 
Lys
 -0.8  -0.2  -1.0  -1.0  -0.1   0.1  -0.3  -0.4  -0.8  -0.6  -0.3  -0.2   0.2   0.6  -0.0 
 -6.2  -2.6  -5.9  -0.7   0.3   0.2   0.1  -1.2  -0.2  -0.3  -0.7  -0.3  -0.8  -1.9  -5.2 
 -1.9  -5.2  -5.4  -5.6  -5.6  -5.4  -5.5  -5.1  -4.9  -4.9  -4.7 
Leu
 -0.9  -1.9  -1.1  -1.1  -3.0  -1.9  -1.2  -1.6  -1.1  -1.3  -1.4  -1.9  -1.3  -1.5  -3.1 
 -6.2  -3.0  -2.3  -0.5  -0.5   0.2  -0.5  -0.3  -1.2   0.0   0.4   0.9   0.5   0.8   0.9 
  0.4   0.7   1.0   0.7   0.5   1.0   1.0   1.1   1.0   1.1   1.0 
Met
 -1.1  -0.7  -1.1  -1.1  -2.9  -1.1  -1.7  -0.3  -1.1  -0.4  -0.4  -1.7  -0.4  -0.2  -3.8 
 -6.2  -1.7  -5.9  -0.2  -0.2   0.5  -0.2  -1.1   0.4  -0.1   0.3   0.2   0.9   0.5   0.2 
  0.5   0.5   0.8   0.1   0.9   0.6  -0.1   0.8   0.8   0.8   1.1 
Asn
 -0.2   0.1   0.2   0.3   0.3   0.4   0.3   0.1   0.2   0.2  -0.4  -0.2  -0.6   0.4   0.5 
  1.7  -0.9  -0.6   0.9   0.6   0.1  -0.1   0.3   0.5   0.3  -0.9  -2.3  -0.7  -0.5  -1.2 
 -2.3  -2.3  -2.3  -5.6  -0.9  -5.4  -1.6  -5.1  -0.9  -1.8  -1.6 
Pro
 -0.1  -0.1   0.2   0.1  -0.6   0.3   0.1   0.4   0.0   0.0  -0.0   0.4   0.4  -0.4  -0.3 
 -6.2  -0.8  -5.9   0.1  -0.3  -1.1  -0.0   0.6   0.4  -0.7  -0.4  -1.0  -0.5  -1.7  -1.7 
 -1.0  -1.3  -2.4  -1.7  -5.6  -2.4  -5.5  -2.2  -1.4  -4.9  -4.7 
Gln
  0.2  -0.8  -0.2  -0.3  -0.4  -0.4  -0.8  -0.6  -0.4  -0.6  -0.6  -0.2  -0.8  -0.1  -0.8 
 -6.2  -1.5  -5.9   0.1  -0.3  -0.0  -0.4   0.1  -0.4  -0.3  -3.9  -1.1  -4.5  -2.2  -5.2 
 -2.2  -5.2  -2.2  -5.6  -5.6  -2.2  -5.5  -5.1  -1.2  -1.0  -1.5 
Arg
 -2.6  -3.8  -2.6  -1.2  -1.2  -1.5  -1.9  -0.8  -1.0  -1.5  -1.2  -1.5  -1.5  -1.0  -1.5 
 -6.2  -2.5  -5.9  -0.5   0.3  -0.4  -0.0  -0.7  -0.9  -1.0  -1.5  -4.6  -1.0  -2.6  -2.6 
 -2.6  -5.2  -5.4  -5.6  -5.6  -5.4  -5.5  -5.1  -4.9  -4.9  -4.7 
Ser
  1.3   1.3   1.2   1.3   1.3   1.5   1.5   1.4   1.3   1.4   1.5   1.4   1.1   0.7   1.2 
  1.7   1.3   1.1   1.0   0.7  -0.3   0.7   0.8   0.8   0.4   0.5   0.1  -0.1  -0.2   0.0 
  0.1  -0.4  -0.2  -0.1  -0.3  -0.2  -0.4  -1.0  -1.1  -0.5  -0.4 
Thr
  1.0   0.8   1.0   0.9   0.7   0.5   1.0   1.0   0.7   0.5   0.7   0.9   0.9   0.6   1.0 
 -6.2  -0.7  -0.7  -0.1  -0.2   0.3   0.4  -0.0   0.4   0.1  -0.3  -0.0   0.3  -0.6  -0.4 
 -0.1  -0.6  -0.2  -0.6  -0.2  -0.8  -0.8  -1.3  -1.2  -0.7  -0.8 
Val
 -0.0  -0.1  -0.3  -0.0  -0.2  -1.3  -0.3  -0.5  -0.3   0.1   0.2  -0.0   0.3  -0.8  -1.0 
 -6.2  -0.7  -5.9   0.2  -0.0   0.4   0.3   0.5  -0.0   0.1   0.4   0.5   0.8   0.7   1.0 
  0.7   1.0   0.9   0.7   0.7   0.8   0.8   0.8   0.2   0.4   0.3 
Trp
 -2.3  -0.4  -1.1  -2.3  -0.4  -4.2  -4.0  -3.2  -1.1  -1.1  -3.3  -3.2  -3.2  -3.2  -3.8 
 -6.2  -5.1  -5.9  -3.2  -0.5  -0.5  -1.1  -0.4  -1.1   1.0  -0.0  -1.1  -0.5  -0.4  -5.2 
 -4.2  -5.2  -1.1  -1.1  -0.0  -5.4   0.0  -5.1  -0.1  -0.6  -0.4 
Tyr
 -1.3  -0.9  -2.0  -0.4  -0.2   0.1  -0.9  -1.3  -1.3  -1.3  -2.0  -0.0  -0.9   1.1  -2.0 
 -6.2  -5.1  -5.9  -0.9  -2.0   0.3  -3.2  -2.0  -0.4  -0.4  -0.2  -0.0  -0.4  -2.0  -0.9 
 -2.0  -2.0  -5.4  -0.8   0.3  -0.3  -0.8  -0.2  -4.9  -0.8  -0.2 

ConsensSequence: s s s s s s s s s s s s s s s s a A s s a s s s g a l l l l a a l a a l l l l l l

Positions with profile component > 1.0 for all amino acid types
Ala  1 2 18 19
Cys 
Asp 
Glu 
Phe  21 23 24
Gly  0 1 2
His 
Ile 
Lys 
Leu  21 22 24 25
Met  25
Asn  0
Pro 
Gln 
Arg 
Ser  -15 -14 -13 -12 -11 -10 -9 -8 -7 -6 -5 -4 -3 -1 0 1 2 3
Thr  -8
Val 
Trp  9
Tyr  -2

Parameters for score calculation:
---------------------------------
TaxonCode: 20
Calculation of profile:
   number of input sequences: 253
   number of regions: 5
   1: from                :  -15
      to                  :  -12
      weight of region    :  0.0
   2: from                :  -11
      to                  :   -2
      weight of region    :  0.5
   3: from                :   -1
      to                  :    2
      weight of region    :  3.0
   4: from                :    3
      to                  :    9
      weight of region    :  0.5
   5: from                :   10
      to                  :   25
      weight of region    :  1.0
   weight of profile      :  0.6
omega-site will be searched in interval [12..40] from C-terminal

Function 0 = Combi_contents_win_penalty : contents and windows of DE in N-terminal region
   Parameters:
   ScFunc[0].from         =           -11
   ScFunc[0].to           =            -1
   ScFunc[0].PropertyName =            DE sections
   ScFunc[0].par1         =         -4.00 penalty
   ScFunc[0].par2         =         50.00 threshold: percentage of DE in N-terminal region
   ScFunc[0].par4         =             3 winlen
   ScFunc[0].par5         =             2 par5 DEs are max allowed per window if par2 is exceeded
   ScFunc[0].wfunc        =          1.00

Function 1 = Lower_Average : hydrophilicity of N-terminal region [-11..-1]
   Parameters:
   ScFunc[1].from         =           -11
   ScFunc[1].to           =            -1
   ScFunc[1].PropertyName =    NAKH920105 hydrophobicity scale
   ScFunc[1].par1         =          5.44 mean hydrophobicity for N-terminal region was calculated
   ScFunc[1].par2         =          1.53 dispersion was calculated
   ScFunc[1].wfunc        =          1.00
   ScFunc[1].distr_type   =       F_GAUSS

Function 2 = PenaltyForBadProfile : penalty for bad Omega-Site
   Parameters:
   ScFunc[2].from         =             0
   ScFunc[2].to           =             2
   ScFunc[2].par1         =          0.10 threshold
   ScFunc[2].par2         =         -4.00 penalty
   ScFunc[2].wfunc        =          1.00

Function 3 = Sum : volume function
   Parameters:
   ScFunc[3].from         =            -1
   ScFunc[3].to           =             2
   ScFunc[3].PropertyName =    HAGECH94_V mean volume of residues
   ScFunc[3].par1         =        386.60 mean volume was calculated
   ScFunc[3].par2         =         44.15 dispersion was calculated
   ScFunc[3].wfunc        =          1.00
   ScFunc[3].distr_type   =       F_GAUSS

Function 4 = Sum3 : volume function for three positions
   Parameters:
   ScFunc[4].from         =             0 Position 1
   ScFunc[4].to           =             2 Position 2
   ScFunc[4].PropertyName =    HAGECH94_V mean volume of residues
   ScFunc[4].par1         =        273.08 mean volume was calculated
   ScFunc[4].par2         =         32.78 dispersion was calculated
   ScFunc[4].par4         =             1 Position 3
   ScFunc[4].wfunc        =          1.00
   ScFunc[4].distr_type   =       F_GAUSS

Function 5 = Sum2 : volume function for two positions
   Parameters:
   ScFunc[5].from         =             0 Position 1
   ScFunc[5].to           =             1 Position 2
   ScFunc[5].PropertyName =    HAGECH94_V mean volume of residues
   ScFunc[5].par1         =        185.07 mean volume was calculated
   ScFunc[5].par2         =         31.22 dispersion was calculated
   ScFunc[5].wfunc        =          0.50
   ScFunc[5].distr_type   =       F_GAUSS

Function 6 = Penalty_Mobility : penalty for more than one residue out of P, T, V, I
   Parameters:
   ScFunc[6].from         =            -1
   ScFunc[6].to           =             2
   ScFunc[6].PropertyName =          PTVI residues
   ScFunc[6].par1         =         -4.00 penalty
   ScFunc[6].wfunc        =          1.00

Function 7 = C_Position : penalty, if site is outside of interval[from..to] relative to C-terminal
   Parameters:
   ScFunc[7].from         =            17
   ScFunc[7].to           =            32
   ScFunc[7].par1         =          0.50 lambda (x < from)
   ScFunc[7].par2         =          1.00 lambda (x > to)
   ScFunc[7].wfunc        =          4.00
   ScFunc[7].distr_type   =   F_BOLTZMANN

Function 8 = Lower_Average : hydrophilicity of spacer region [3..8]
   Parameters:
   ScFunc[8].from         =             3 (after omega-site)
   ScFunc[8].to           =             8
   ScFunc[8].PropertyName =    NAKH920105 hydrophobicity scale
   ScFunc[8].par1         =          6.91 mean hydrophobicity for spacer region was calculated
   ScFunc[8].par2         =          2.38 dispersion was calculated
   ScFunc[8].wfunc        =          3.00
   ScFunc[8].distr_type   =       F_GAUSS

Function 9 = Lower_Average : function for spacer volume (mean for positions 3..8)
   Parameters:
   ScFunc[9].from         =             3 (after omega-site)
   ScFunc[9].to           =             8
   ScFunc[9].PropertyName =    HAGECH94_V mean volume of residues
   ScFunc[9].par1         =        129.82 mean volume for spacer region was calculated
   ScFunc[9].par2         =         16.22 dispersion was calculated
   ScFunc[9].wfunc        =          1.00
   ScFunc[9].distr_type   =       F_GAUSS

Function 10 = Charged_AA_Penalty_Spacer : penalty for charged AA (DEKRH) in spacer (H=2/3)
   Parameters:
   ScFunc[10].from         =             3
   ScFunc[10].to           =            10
   ScFunc[10].par1         =         -4.00 penalty
   ScFunc[10].par4         =             3 threshold: max number of charged AA in spacer
   ScFunc[10].wfunc        =          1.00

Function 11 = Flexibility_Spacer : contents of PVI in spacer
   Parameters:
   ScFunc[11].from         =             3
   ScFunc[11].to           =             8
   ScFunc[11].par1         =         -4.00 penalty
   ScFunc[11].par4         =             4 par4 non-flexible residues are max allowed
   ScFunc[11].wfunc        =          1.00

Function 12 = PenaltyForBadProfile : penalty for bad profile in hydrophobic tail
   Parameters:
   ScFunc[12].from         =            10
   ScFunc[12].to           =   PROFILE_END
   ScFunc[12].par1         =          0.20 threshold
   ScFunc[12].par2         =        -12.00 penalty
   ScFunc[12].wfunc        =          1.00

 Function 13 = Higher_Average: function for hydrophobic region [10..C_end] (for at least the par4=8 last AAs)
   Parameters:
   ScFunc[13].from         =            10
   ScFunc[13].to           = SEQUENCE_CEND
   ScFunc[13].PropertyName =    NAKH920105 hydrophobicity scale
   ScFunc[13].par1         =         12.00 mean hydrophobicity for at least the 8 last AAs was calculated
   ScFunc[13].par2         =          1.72 dispersion was calculated
   ScFunc[13].par4         =             8 minimal length of hydrophobic region
   ScFunc[13].wfunc        =          1.00
   ScFunc[13].distr_type   =       F_GAUSS

Function 14 = Higher_Average : function for hydrophobic tail [26..C_end](for at least the par4=8 last AAs)
   Parameters:
   ScFunc[14].from         =            26 (after profile-end)
   ScFunc[14].to           = SEQUENCE_CEND
   ScFunc[14].PropertyName =    NAKH920105 hydrophobicity scale
   ScFunc[14].par1         =         12.63 mean hydrophobicity for at least the 8 last AAs was calculated
   ScFunc[14].par2         =          2.40 dispersion was calculated
   ScFunc[14].par4         =             8 minimal length of tail for calculation of hydrophobicity parameters
   ScFunc[14].wfunc        =          1.00
   ScFunc[14].distr_type   =       F_GAUSS

Function 15 = Sliding_Window : penalty for too short even hydrophobic section
   Parameters:
   ScFunc[15].from         =             9
   ScFunc[15].to           = SEQUENCE_CEND
   ScFunc[15].PropertyName =    NAKH920105 hydrophobicity scale
   ScFunc[15].par1         =          9.00 threshold for windows containig Leu (min mean window hydrophobicity)
   ScFunc[15].par2         =          6.00 threshold for windows without Leu
   ScFunc[15].par3         =        -12.00 penalty
   ScFunc[15].par4         =             4 winlen
   ScFunc[15].par5         =             5 min number of windows one after another
   ScFunc[15].wfunc        =          1.00

Function 16 = Post_Sliding_Window_Polar_Penalty : penalty for polar windows in the hydrophobic tail
   Parameters:
   ScFunc[16].from         =            10
   ScFunc[16].to           = SEQUENCE_CEND
   ScFunc[16].PropertyName =    NAKH920105 hydrophobicity scale
   ScFunc[16].par1         =          1.50 minimum of mean hydrophobicity per window
   ScFunc[16].par2         =         -4.00 penalty
   ScFunc[16].par3         =          0.50 threshold for extreme windows
   ScFunc[16].par4         =             3 winlen
   ScFunc[16].wfunc        =          1.00

Function 17 = Post_Sliding_Window_SGC_Penalty : penalty for too long SGC - sections (len = par4) in hydrophobic tail
   Parameters:
   ScFunc[17].from         =             9
   ScFunc[17].to           = SEQUENCE_CEND
   ScFunc[17].PropertyName =    NAKH920105 hydrophobicity scale
   ScFunc[17].par1         =        -12.00 penalty
   ScFunc[17].par2         =          8.20 threshold for hydrophobicity of a window with len=(winlen+1)
   ScFunc[17].par4         =             3 winlen
   ScFunc[17].par5         =             2 are max allowed per window 
   ScFunc[17].wfunc        =          1.00

Function 18 = LVI_contents : contents of Leu, Val, Ile in the hydrophobic tail
   Parameters:
   ScFunc[18].from         =            10
   ScFunc[18].to           = SEQUENCE_CEND
   ScFunc[18].par1         =         20.00 threshold
   ScFunc[18].par2         =        -12.00 penalty
   ScFunc[18].wfunc        =          1.00

Function 19 = Combi_FWHY_contents_2win : penalty if more than 40% WHFY and 2 disconnected windows (l=4) with > 2 aromats
   Parameters:
   ScFunc[19].from         =             9
   ScFunc[19].to           = SEQUENCE_CEND
   ScFunc[19].PropertyName =    ZVEL_AROMA WHFY sections
   ScFunc[19].par1         =         40.00 threshold: percentage of aromats in tail
   ScFunc[19].par2         =        -12.00 penalty
   ScFunc[19].par4         =             4 winlen
   ScFunc[19].par5         =             2 aromats are max allowed per window if par1 is exceeded
   ScFunc[19].wfunc        =          1.00

Function 20 = Sliding_Window : penalty for small (according to volume) windows in tail 
   Parameters:
   ScFunc[20].from         =             9
   ScFunc[20].to           = SEQUENCE_CEND
   ScFunc[20].PropertyName =    HAGECH94_V volume scale
   ScFunc[20].par1         =        110.00 threshold (min mean window volume)
   ScFunc[20].par2         =        -12.00 penalty
   ScFunc[20].par4         =             4 winlen
   ScFunc[20].par5         =             5 min number of windows one after another
   ScFunc[20].wfunc        =          0.00

Function 21 = Sum2 : volume function for two positions
   Parameters:
   ScFunc[21].from         =             1 Position 1
   ScFunc[21].to           =             2 Position 2
   ScFunc[21].PropertyName =    HAGECH94_V mean volume of residues
   ScFunc[21].par1         =        178.17 mean volume was calculated
   ScFunc[21].par2         =         26.73 dispersion was calculated
   ScFunc[21].wfunc        =          0.50
   ScFunc[21].distr_type   =       F_GAUSS



Last modified: 11th August 2003