RID=1088427889-31901-55135666612.BLASTQ4, NP_651134.1_220-424 BLASTP 2.2.9 [May-01-2004] 

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


RID: 1088427889-31901-55135666612.BLASTQ4


Query= NP_651134.1_220-424
         (205 letters)



Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples 
           1,875,456 sequences; 622,745,705 total letters


 
If you have any problems or questions with the results of this search 
please refer to the BLAST FAQs



Results of PSI-Blast iteration 1

Distribution of 11 Blast Hits on the Query Sequence

Legend:
New sequence mark - means that the alignment score was below the threshold on the previous iteration 
Checked mark - means that the alignment was checked on the previous iteration 



Hit list size 

Sequences with E-value BETTER than threshold

                                                                     Score    E
Sequences producing significant alignments:                           (bits) Value



New sequence mark  gi|24649260|ref|NP_651134.1|  CG4449-PA [Drosophila melanogaster]...   395   e-109 LocusLink info

New sequence mark  gi|21430264|gb|AAM50810.1|  LD33701p [Drosophila melanogaster]         395   e-109 LocusLink info

New sequence mark  gi|31200225|ref|XP_309060.1|  ENSANGP00000019625 [Anopheles gambi...   103   3e-21 




Sequences with E-value WORSE than threshold




   gi|45751566|gb|AAH68007.1|  FLJ14639 protein [Homo sapiens]             36   0.58  LocusLink info

   gi|14042298|dbj|BAB55189.1|  unnamed protein product [Homo sapien...    36   0.58  LocusLink info

   gi|11041477|dbj|BAB17279.1|  hypothetical protein [Macaca fascicu...    35   0.69  

   gi|21360814|gb|AAM49721.1|  hypothetical protein [Homo sapiens]         35   1.2   LocusLink info

   gi|46447823|ref|NP_116204.3|  nuclear factor of activated T-cells...    35   1.3   LocusLink info

   gi|18408968|ref|NP_564924.1|  ubiquitin-related [Arabidopsis thal...    34   2.4   

   gi|33304726|gb|AAP34642.1|  small ubiquitin-like modifier [Bigelo...    33   4.8   

   gi|38075215|ref|XP_130609.3|  RIKEN cDNA 4921517L17 [Mus musculus]      32   8.0   LocusLink info




Alignments











>gi|24649260|ref|NP_651134.1|  LocusLink info CG4449-PA [Drosophila melanogaster]
 gi|23172035|gb|AAF56116.2|  LocusLink info CG4449-PA [Drosophila melanogaster]
          Length = 424

 Score =  395 bits (1016), Expect = e-109
 Identities = 205/205 (100%), Positives = 205/205 (100%)

Query: 1   PRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHL 60
           PRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHL
Sbjct: 220 PRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHL 279

Query: 61  FKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDY 120
           FKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDY
Sbjct: 280 FKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDY 339

Query: 121 NPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGD 180
           NPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGD
Sbjct: 340 NPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGD 399

Query: 181 LLDPNDTPNNQDMEGNEVIDLKIKA 205
           LLDPNDTPNNQDMEGNEVIDLKIKA
Sbjct: 400 LLDPNDTPNNQDMEGNEVIDLKIKA 424





>gi|21430264|gb|AAM50810.1|  LocusLink info LD33701p [Drosophila melanogaster]
          Length = 422

 Score =  395 bits (1014), Expect = e-109
 Identities = 205/205 (100%), Positives = 205/205 (100%)

Query: 1   PRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHL 60
           PRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHL
Sbjct: 218 PRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHL 277

Query: 61  FKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDY 120
           FKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDY
Sbjct: 278 FKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDY 337

Query: 121 NPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGD 180
           NPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGD
Sbjct: 338 NPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGD 397

Query: 181 LLDPNDTPNNQDMEGNEVIDLKIKA 205
           LLDPNDTPNNQDMEGNEVIDLKIKA
Sbjct: 398 LLDPNDTPNNQDMEGNEVIDLKIKA 422





>gi|31200225|ref|XP_309060.1|   ENSANGP00000019625 [Anopheles gambiae]
 gi|21292650|gb|EAA04795.1|   ENSANGP00000019625 [Anopheles gambiae str. PEST]
          Length = 199

 Score =  103 bits (256), Expect = 3e-21
 Identities = 63/200 (31%), Positives = 106/200 (53%), Gaps = 8/200 (4%)

Query: 7   VNLDSEDE---GEKEAPPVVEEENI-FDNDNPTIEVALSWLGEIQIYKLRQHQKFKHLFK 62
           ++LDS+DE   G  E  P     N  F+ +N  + + + W   I+ +  R++QKF+ +F 
Sbjct: 1   ISLDSDDESGDGAVELEPSFSAANSSFETENYEMRIKIKWGRGIETFVHRRYQKFEDIFN 60

Query: 63  ELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDYNP 122
           +LA++   D   I +++    V   DTP SI  K    + G   K+       A    + 
Sbjct: 61  QLAAKESADRACIFLNLDDRIVYASDTPDSIDYKPHQFIAGRILKTKAPILPTA----HG 116

Query: 123 EALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGDLL 182
            +         +KVQ +K K PL + + K     ++ IKCAEEL C+P+ I+L+FDG+L+
Sbjct: 117 SSTGHNSNMITLKVQMEKRKQPLRLQIDKNQTMSVLVIKCAEELKCEPKDIRLYFDGELV 176

Query: 183 DPNDTPNNQDMEGNEVIDLK 202
             ND P + D+EG+E++D++
Sbjct: 177 GNNDKPEDLDLEGDEILDIR 196





>gi|45751566|gb|AAH68007.1|  LocusLink info FLJ14639 protein [Homo sapiens]
          Length = 127

 Score = 35.8 bits (81), Expect = 0.58
 Identities = 19/81 (23%), Positives = 37/81 (45%), Gaps = 1/81 (1%)

Query: 121 NPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGD 180
           +PEA     ++ Q++VQ  +    L + + +    K +     E +    R +  FFDG 
Sbjct: 46  SPEA-TETSQQLQLRVQGKEKHQTLEVSLSRDSPLKTLMSHYEEAMGLSGRKLSFFFDGT 104

Query: 181 LLDPNDTPNNQDMEGNEVIDL 201
            L   + P +  ME  ++I++
Sbjct: 105 KLSGRELPADLGMESGDLIEV 125





>gi|14042298|dbj|BAB55189.1|  LocusLink info unnamed protein product [Homo sapiens]
 gi|17390726|gb|AAH18311.1|  LocusLink info FLJ14639 protein [Homo sapiens]
 gi|18204286|gb|AAH21551.1|  LocusLink info FLJ14639 protein [Homo sapiens]
          Length = 138

 Score = 35.8 bits (81), Expect = 0.58
 Identities = 19/81 (23%), Positives = 37/81 (45%), Gaps = 1/81 (1%)

Query: 121 NPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGD 180
           +PEA     ++ Q++VQ  +    L + + +    K +     E +    R +  FFDG 
Sbjct: 57  SPEA-TETSQQLQLRVQGKEKHQTLEVSLSRDSPLKTLMSHYEEAMGLSGRKLSFFFDGT 115

Query: 181 LLDPNDTPNNQDMEGNEVIDL 201
            L   + P +  ME  ++I++
Sbjct: 116 KLSGRELPADLGMESGDLIEV 136





>gi|11041477|dbj|BAB17279.1|   hypothetical protein [Macaca fascicularis]
          Length = 408

 Score = 35.4 bits (80), Expect = 0.69
 Identities = 32/156 (20%), Positives = 61/156 (39%), Gaps = 23/156 (14%)

Query: 51  LRQHQKFKHLFKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHN 110
           LR  +  + +   +A+  G+  + I +      + P  TP ++ L               
Sbjct: 269 LRMSEPLQSVVDHMATHLGVSPSRILLLFGETELSPTATPRTLKL--------------- 313

Query: 111 NNNVAAKID-----YNPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEE 165
              VA  ID      +PEA     ++ Q++VQ  +    L + + +    K +     E 
Sbjct: 314 --GVADIIDCVVLASSPEA-TETSRQLQLRVQGKEKHQTLEVSLSRDSPLKTLMSHYEEA 370

Query: 166 LNCDPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDL 201
           +    R +  FFDG  L   + P +  ME  ++I++
Sbjct: 371 MGLSGRKLSFFFDGTKLSGRELPADLGMESGDLIEV 406





>gi|21360814|gb|AAM49721.1|  LocusLink info hypothetical protein [Homo sapiens]
          Length = 419

 Score = 34.7 bits (78), Expect = 1.2
 Identities = 32/156 (20%), Positives = 61/156 (39%), Gaps = 23/156 (14%)

Query: 51  LRQHQKFKHLFKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHN 110
           LR  +  + +   +A+  G+  + I +      + P  TP ++ L               
Sbjct: 280 LRMSEPLQSVVDHMATHLGVSPSRILLLFGETELSPTATPRTLKL--------------- 324

Query: 111 NNNVAAKID-----YNPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEE 165
              VA  ID      +PEA     ++ Q++VQ  +    L + + +    K +     E 
Sbjct: 325 --GVADIIDCVVLTSSPEA-TETSQQLQLRVQGKEKHQTLEVSLSRDSPLKTLMSHYEEA 381

Query: 166 LNCDPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDL 201
           +    R +  FFDG  L   + P +  ME  ++I++
Sbjct: 382 MGLSGRKLSFFFDGTKLSGRELPADLGMESGDLIEV 417





>gi|46447823|ref|NP_116204.3|  LocusLink info nuclear factor of activated T-cells, cytoplasmic,
           calcineurin-dependent 2 interacting protein [Homo
           sapiens]
 gi|22760416|dbj|BAC11189.1|  LocusLink info unnamed protein product [Homo sapiens]
          Length = 419

 Score = 34.7 bits (78), Expect = 1.3
 Identities = 32/156 (20%), Positives = 61/156 (39%), Gaps = 23/156 (14%)

Query: 51  LRQHQKFKHLFKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHN 110
           LR  +  + +   +A+  G+  + I +      + P  TP ++ L               
Sbjct: 280 LRMSEPLQSVVDHMATHLGVSPSRILLLFGETELSPTATPRTLKL--------------- 324

Query: 111 NNNVAAKID-----YNPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEE 165
              VA  ID      +PEA     ++ Q++VQ  +    L + + +    K +     E 
Sbjct: 325 --GVADIIDCVVLTSSPEA-TETSQQLQLRVQGKEKHQTLEVSLSRDSPLKTLMSHYEEA 381

Query: 166 LNCDPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDL 201
           +    R +  FFDG  L   + P +  ME  ++I++
Sbjct: 382 MGLSGRKLSFFFDGTKLSGRELPADLGMESGDLIEV 417





>gi|18408968|ref|NP_564924.1|   ubiquitin-related [Arabidopsis thaliana]
 gi|21554742|gb|AAM63677.1|   unknown [Arabidopsis thaliana]
          Length = 215

 Score = 33.9 bits (76), Expect = 2.4
 Identities = 14/36 (38%), Positives = 24/36 (66%)

Query: 169 DPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDLKIK 204
           DP+ +   FDGD +DP+ TP+   ME +++I++  K
Sbjct: 178 DPQNLVFIFDGDKIDPSTTPSELGMEDHDMIEVHTK 213





>gi|33304726|gb|AAP34642.1|   small ubiquitin-like modifier [Bigelowiella natans]
          Length = 90

 Score = 32.7 bits (73), Expect = 4.8
 Identities = 15/56 (26%), Positives = 31/56 (55%)

Query: 149 MKKTDNFKIIFIKCAEELNCDPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDLKIK 204
           +KKT  FK +     +++  +  +++  FDGD +  + TP + DM+  + ID  ++
Sbjct: 29  VKKTTPFKRLMEAYCQKVGAEKSSVRFLFDGDRISGDQTPADLDMQDEDEIDAMVQ 84





>gi|38075215|ref|XP_130609.3|  LocusLink info RIKEN cDNA 4921517L17 [Mus musculus]
          Length = 661

 Score = 32.0 bits (71), Expect = 8.0
 Identities = 17/64 (26%), Positives = 33/64 (51%), Gaps = 3/64 (4%)

Query: 10  DSEDEGEKEAPPVVEE---ENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHLFKELAS 66
           DS  E +KE+P  ++E   E     D P +++ LSW  +I+ +  ++ +K   L  ++  
Sbjct: 59  DSRSEEDKESPASIKEIKAETPQPKDRPGVQIKLSWSQKIKSWTAKKKRKLYQLVIDIIM 118

Query: 67  RNGI 70
            N +
Sbjct: 119 MNRV 122










  Database: All non-redundant GenBank CDS
  translations+PDB+SwissProt+PIR+PRF excluding environmental samples
    Posted date:  Jun 28, 2004 12:15 AM
  Number of letters in database: 622,745,705
  Number of sequences in database:  1,875,456
  
Lambda     K      H
   0.316    0.137    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,558,079
Number of Sequences: 1875456
Number of extensions: 2546840
Number of successful extensions: 4216
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 4203
Number of HSP's gapped (non-prelim): 11
length of query: 205
length of database: 622,745,705
effective HSP length: 119
effective length of query: 86
effective length of database: 399,566,441
effective search space: 34362713926
effective search space used: 34362713926
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 71 (32.0 bits)