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GPI Anchor Biosynthesis Report: XP_325888.1 (PIG-S family, Neurospora crassa)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (568 letters)

Database: nr
           1,543,518 sequences; 504,497,942 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 25 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|XP_325888.1| hypothetical protein [Neurospora crassa] >gi|28... 828 0.0
emb|CAD60556.1| unnamed protein product [Podospora anserina] 722 0.0
gb|EAA57290.1| hypothetical protein MG08259.4 [Magnaporthe grise... 700 0.0
gb|AAO39601.1| GM10512p [Drosophila melanogaster] 587 e-166
ref|NP_733060.1| CG31120-PB [Drosophila melanogaster] >gi|169020... 584 e-165
ref|XP_311969.1| ENSANGP00000010967 [Anopheles gambiae] >gi|2129... 430 e-119
ref|XP_220633.2| similar to phosphatidylinositol glycan class S ... 423 e-117
gb|AAQ89470.1| PIGS [Homo sapiens] 419 e-115
ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapie... 417 e-115
dbj|BAC11615.1| unnamed protein product [Homo sapiens] 410 e-113
ref|XP_203412.1| similar to phosphatidylinositol glycan class S ... 405 e-111
ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Sacc... 380 e-104
ref|NP_187374.2| expressed protein [Arabidopsis thaliana] >gi|17... 351 3e-95
ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe... 343 5e-93
gb|AAF20228.1| unknown protein [Arabidopsis thaliana] 314 5e-84
gb|AAH01319.2| PIGS protein [Homo sapiens] 255 2e-66
dbj|BAB92563.1| P0497A05.6 [Oryza sativa (japonica cultivar-group)] 244 5e-63
ref|XP_142436.3| similar to phosphatidylinositol glycan class S ... 195 2e-48
dbj|BAB70938.1| unnamed protein product [Homo sapiens] 188 3e-46
emb|CAE45912.1| hypothetical protein [Homo sapiens] 182 2e-44
ref|NP_501852.1| phosphatidylinositol glycan class S like (4K912... 125 4e-27
Alignments
>ref|XP_325888.1| hypothetical protein [Neurospora crassa]
 gb|EAA30387.1| hypothetical protein [Neurospora crassa]
          Length = 568

 Score =  828 bits (2116), Expect = 0.0
 Identities = 556/556 (100%), Positives = 556/556 (100%)

Query: 1   MFARRPIDHGAAGDTLSSETQMATMTSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRRI 60
           MFARRPIDHGAAGDTLSSETQMATMTSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRRI
Sbjct: 1   MFARRPIDHGAAGDTLSSETQMATMTSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRRI 60

Query: 61  WVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQANSLPE 120
           WVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQANSLPE
Sbjct: 61  WVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQANSLPE 120

Query: 121 QEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTIRLLPGDAVTATL 180
           QEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTIRLLPGDAVTATL
Sbjct: 121 QEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTIRLLPGDAVTATL 180

Query: 181 DPYEPVLDITYPPNAAPSATSASSSLATYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRP 240
           DPYEPVLDITYPPNAAPSATSASSSLATYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRP
Sbjct: 181 DPYEPVLDITYPPNAAPSATSASSSLATYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRP 240

Query: 241 QSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSSWDIEAAIQDYLKPVLGLLSP 300
           QSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSSWDIEAAIQDYLKPVLGLLSP
Sbjct: 241 QSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSSWDIEAAIQDYLKPVLGLLSP 300

Query: 301 IHNFTIDTQVQLYASPGVQNQVLSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTI 360
           IHNFTIDTQVQLYASPGVQNQVLSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTI
Sbjct: 301 IHNFTIDTQVQLYASPGVQNQVLSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTI 360

Query: 361 ALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSLPHDTAHVSVDALKQPLLTFTSHLLSL 420
           ALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSLPHDTAHVSVDALKQPLLTFTSHLLSL
Sbjct: 361 ALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSLPHDTAHVSVDALKQPLLTFTSHLLSL 420

Query: 421 TGTPSSGSLPLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIV 480
           TGTPSSGSLPLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIV
Sbjct: 421 TGTPSSGSLPLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIV 480

Query: 481 HLRQACETLGEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPL 540
           HLRQACETLGEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPL
Sbjct: 481 HLRQACETLGEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPL 540

Query: 541 VMGMLNELKAWRKRKR 556
           VMGMLNELKAWRKRKR
Sbjct: 541 VMGMLNELKAWRKRKR 556
>emb|CAD60556.1| unnamed protein product [Podospora anserina]
          Length = 542

 Score =  722 bits (1846), Expect = 0.0
 Identities = 417/537 (77%), Positives = 469/537 (86%), Gaps = 14/537 (2%)

Query: 32  TPLSSATAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPL 91
             ++    A TS++K PPPEK SD+RRR  VI+SFWLIVLLLGLP+WWKTTTI+RA+LPL
Sbjct: 16  ATIAPKQEAPTSSKKLPPPEKPSDVRRRSHVILSFWLIVLLLGLPIWWKTTTIYRADLPL 75

Query: 92  KEMLDWADGKACRPVFPLRISIQANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSS 151
           ++ML+WADGKACRPVFPLRISIQA SL +QEAQNLLRLTQHALDDLNDF+GHHLRLQ+  
Sbjct: 76  EDMLEWADGKACRPVFPLRISIQATSLQDQEAQNLLRLTQHALDDLNDFAGHHLRLQMGD 135

Query: 152 PAAPQKRNDEPD--TALTIRLLPGDAVTATLDPYEPVLDITYPPNAAPSATSASSSLATY 209
                ++ D  D  +ALTIRL PG+  TATLDPYEP+LDITYP N  PS T++SS+LATY
Sbjct: 136 -----EKQDSADNGSALTIRLTPGEGTTATLDPYEPILDITYPQNTIPSPTASSSALATY 190

Query: 210 VAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTF 269
           +A QLR+TFAEEQA ISYLLS+NS+P+++RP  L+PE AE+LAKRTTRSLKYAPTYHLTF
Sbjct: 191 IASQLRTTFAEEQATISYLLSTNSMPSEHRPLGLSPETAEALAKRTTRSLKYAPTYHLTF 250

Query: 270 SLFTSGPLPSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQVLSKDDLS 329
           SLFTSGPLPSSWDIE AI++Y+KPVL +LSPIHNFTIDTQVQLYASPGVQNQVL+K+DLS
Sbjct: 251 SLFTSGPLPSSWDIETAIEEYMKPVLDVLSPIHNFTIDTQVQLYASPGVQNQVLNKEDLS 310

Query: 330 SFINAAEWPLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTV 389
           SFINAAEWPLSPSIGGAPT+NFIVFVGNQTIA  S+ A         TS SWLIPQWGTV
Sbjct: 311 SFINAAEWPLSPSIGGAPTINFIVFVGNQTIASPSQEA-------APTSHSWLIPQWGTV 363

Query: 390 YLLSLPHDTAHVSVDALKQPLLTFTSHLLSLTGTPSSGSLPLRLSTLSRIRSADLLLRAS 449
           +LLSLP DT HV+   LKQPLLTFTSHLLSLTGTP SGSLPLRLSTL+RIRSADLLLRAS
Sbjct: 364 FLLSLPGDTTHVAATTLKQPLLTFTSHLLSLTGTPESGSLPLRLSTLARIRSADLLLRAS 423

Query: 450 STLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEAEGLEHARIAEAEAERAF 509
           STLGSLARLA+AL SISIPSSVADGV+KT+ HLR AC+ LG  EGL HARIAE EAERAF
Sbjct: 424 STLGSLARLAMALPSISIPSSVADGVTKTMSHLRLACDNLGGTEGLAHARIAEVEAERAF 483

Query: 510 FEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELKAWRKRKREAAEKGKEKK 566
           FEKSMVGQLYFPDEHK+AVYLPLLGPVAVPLVMG++NE+KAWRKR+RE AEKG EKK
Sbjct: 484 FEKSMVGQLYFPDEHKIAVYLPLLGPVAVPLVMGLINEIKAWRKRRRERAEKGAEKK 540
>gb|EAA57290.1| hypothetical protein MG08259.4 [Magnaporthe grisea 70-15]
          Length = 558

 Score =  700 bits (1788), Expect = 0.0
 Identities = 322/544 (59%), Positives = 406/544 (74%), Gaps = 16/544 (2%)

Query: 26  TSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIH 85
           + + VA PL +       T K+PPPEK +D+RRR +VI+SFW ++L +GLP+WW TT+++
Sbjct: 27  SGARVAAPLPA-----NKTNKDPPPEKATDVRRRSYVILSFWALILSVGLPIWWHTTSVY 81

Query: 86  RANLPLKEMLDWADGKACRPVFPLRISIQANSLPEQEAQNLLRLTQHALDDLNDFSGHHL 145
           RA+LPL EM+DWADG+ACRP FPL I+I+  ++ + E   L+++TQ  LD L++ +    
Sbjct: 82  RASLPLDEMMDWADGRACRPTFPLTIAIEKGNMRQDETDVLIQMTQMVLDSLSESTDSTD 141

Query: 146 RLQLSSPAAPQKRNDEPDTALTIRLLPGDAVTATLDPYEPVLDITYPPNAAPSATSASSS 205
           R QL    A +   D+   ALT+RL PGD  T  L P +P+L +TY  N  P     S  
Sbjct: 142 R-QLRLTVADETSVDDEKIALTVRLNPGDTTTTALQPEQPILQVTYAANTLP-----SPG 195

Query: 206 LATYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTY 265
           LA+ +A  + +  +EEQA++S L++  S  T      ++P  A ++AKR+TRSLKYAPTY
Sbjct: 196 LASRIARDVHAVLSEEQAVVSNLVAEASGSTKPSLSGVSPAVAAAVAKRSTRSLKYAPTY 255

Query: 266 HLTFSLFTSGPLPSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQVLSK 325
           HLTFSLFT+G  PSSWDI+AA++ Y++PVL +LSPIHNFTIDTQVQLYA+PGVQ+ VLSK
Sbjct: 256 HLTFSLFTAGSSPSSWDIDAAVEQYMRPVLDVLSPIHNFTIDTQVQLYAAPGVQSDVLSK 315

Query: 326 DDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQ 385
           DDLSSFINAAEWPLSPSIGGAPT+NF+VFVGNQ+I+L +  A   A     T+QSWLIPQ
Sbjct: 316 DDLSSFINAAEWPLSPSIGGAPTINFVVFVGNQSISLPTTEAGSDATA---TAQSWLIPQ 372

Query: 386 WGTVYLLSLPHDT-AHVSVDALKQPLLTFTSHLLSLTGTP-SSGSLPLRLSTLSRIRSAD 443
           WGTVYL+    +    V   +L+ P+ TFT+HLLSL GTP +SGSLPLRL+ L RIRSAD
Sbjct: 373 WGTVYLVPQTAENANKVPAQSLRAPMATFTAHLLSLLGTPETSGSLPLRLAGLRRIRSAD 432

Query: 444 LLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEAEGLEHARIAEA 503
           L+LRASS+LGSLARL+ AL  I+IP SVADGVS TI HLRQAC+ LG + GL HARIA+ 
Sbjct: 433 LVLRASSSLGSLARLSRALPGIAIPRSVADGVSTTIRHLRQACDALGTSAGLAHARIAQV 492

Query: 504 EAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELKAWRKRKREAAEKGK 563
            A++AFFEKSMVGQLYFPDEHKVAVYLPLLGPV VPLVMG+LNELKAWRKR++E A   K
Sbjct: 493 AADKAFFEKSMVGQLYFPDEHKVAVYLPLLGPVGVPLVMGLLNELKAWRKRRQEKAAAEK 552

Query: 564 EKKK 567
             KK
Sbjct: 553 TGKK 556
>gb|AAO39601.1| GM10512p [Drosophila melanogaster]
          Length = 559

 Score =  587 bits (1497), Expect = e-166
 Identities = 129/571 (22%), Positives = 240/571 (41%), Gaps = 88/571 (15%)

Query: 36  SATAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEML 95
           S     +S+  +P  +   + + RI   ++F ++++ +G+P+WW+TTT++R NLP  E+L
Sbjct: 22  SIMETSSSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEIL 81

Query: 96  DWADGKACRPVFPLRISIQANSLPEQEA--QNLLRLTQHALDDLNDFSGHHLRLQLSSPA 153
             +         P++ ++Q     +Q +  Q L+   Q+A  D   +S    +L      
Sbjct: 82  SLSK-------IPIKTAVQVAIYTQQTSRGQLLIAELQNAFSDNEIWSVEFKQLS----P 130

Query: 154 APQKRNDEPDTALTIRLLP------GDAVTATLDPYEPVLDITYPPNAAPSATSASSSLA 207
            P  +      AL   LL       GD +       +  L +T   +A   + + S+ +A
Sbjct: 131 TPMTQEAHTPAALEKLLLENHVQSVGDFMFIEWPKLQEELLLTTERSALMRSDTPSNKIA 190

Query: 208 TYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHL 267
             +  ++  T+      I+ +LS++                    +   +S    P Y +
Sbjct: 191 QLLHAKILQTYR-----INQILSTDE-------------------RMGAKSEAPQPAYDV 226

Query: 268 TFSLFTSGPL--PSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYAS-----PGVQN 320
             S+    P    + W+I  A++ Y++P L  +S + N+T+ +Q +   +       V++
Sbjct: 227 IVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVRD 286

Query: 321 Q-------VLSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEG 373
           Q        L +  L   + +    LS S    P +N +V++    IA    + ++S + 
Sbjct: 287 QSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCHIA--PLHIYNSKDQ 344

Query: 374 GLTTS--QSWLIPQWGTVYLLSLPHDTA------------HVSVDALKQPLLTFTSHLLS 419
            LT +   +++ P WG   + + P                HVS     Q +L     LL 
Sbjct: 345 RLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLD 404

Query: 420 LTGTPSSGSL---------PLRLSTLSRIRSADL--LLRASSTLGSLARLALALKSISIP 468
           ++       +         P R    + +R + +  +  ASSTL SL +L   +  I I 
Sbjct: 405 ISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSLIKLLDQISYIVID 464

Query: 469 SSVADGVSKTIVHLRQACETL---GEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHK 525
             V   ++ +   +  A   L     A+    A+ A   +ER FF+ S++ QLYFPDE K
Sbjct: 465 DEVGTAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGFFDASLLAQLYFPDEQK 524

Query: 526 VAVYLPLLGPVAVPLVMGMLNELKAWRKRKR 556
            A+Y+PL  P+ VP V+   N L+   + +R
Sbjct: 525 YAIYIPLFLPIMVP-VLSSFNMLRGVLQARR 554
>ref|NP_733060.1| CG31120-PB [Drosophila melanogaster]
 gb|AAL27645.1| SD10476p [Drosophila melanogaster]
 gb|AAF56366.2| CG31120-PB [Drosophila melanogaster]
          Length = 536

 Score =  584 bits (1491), Expect = e-165
 Identities = 128/569 (22%), Positives = 239/569 (41%), Gaps = 88/569 (15%)

Query: 38  TAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDW 97
               +S+  +P  +   + + RI   ++F ++++ +G+P+WW+TTT++R NLP  E+L  
Sbjct: 1   METSSSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSL 60

Query: 98  ADGKACRPVFPLRISIQANSLPEQEA--QNLLRLTQHALDDLNDFSGHHLRLQLSSPAAP 155
           +         P++ ++Q     +Q +  Q L+   Q+A  D   +S    +L       P
Sbjct: 61  SK-------IPIKTAVQVAIYTQQTSRGQLLIAELQNAFSDNEIWSVEFKQLS----PTP 109

Query: 156 QKRNDEPDTALTIRLLP------GDAVTATLDPYEPVLDITYPPNAAPSATSASSSLATY 209
             +      AL   LL       GD +       +  L +T   +A   + + S+ +A  
Sbjct: 110 MTQEAHTPAALEKLLLENHVQSVGDFMFIEWPKLQEELLLTTERSALMRSDTPSNKIAQL 169

Query: 210 VAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTF 269
           +  ++  T+      I+ +LS++                    +   +S    P Y +  
Sbjct: 170 LHAKILQTYR-----INQILSTDE-------------------RMGAKSEAPQPAYDVIV 205

Query: 270 SLFTSGPL--PSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYAS-----PGVQNQ- 321
           S+    P    + W+I  A++ Y++P L  +S + N+T+ +Q +   +       V++Q 
Sbjct: 206 SVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVRDQS 265

Query: 322 ------VLSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGL 375
                  L +  L   + +    LS S    P +N +V++    IA    + ++S +  L
Sbjct: 266 KLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCHIA--PLHIYNSKDQRL 323

Query: 376 TTS--QSWLIPQWGTVYLLSLPHDTA------------HVSVDALKQPLLTFTSHLLSLT 421
           T +   +++ P WG   + + P                HVS     Q +L     LL ++
Sbjct: 324 TRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLDIS 383

Query: 422 GTPSSGSL---------PLRLSTLSRIRSADL--LLRASSTLGSLARLALALKSISIPSS 470
                  +         P R    + +R + +  +  ASSTL SL +L   +  I I   
Sbjct: 384 SELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSLIKLLDQISYIVIDDE 443

Query: 471 VADGVSKTIVHLRQACETL---GEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVA 527
           V   ++ +   +  A   L     A+    A+ A   +ER FF+ S++ QLYFPDE K A
Sbjct: 444 VGAAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGFFDASLLAQLYFPDEQKYA 503

Query: 528 VYLPLLGPVAVPLVMGMLNELKAWRKRKR 556
           +Y+PL  P+ VP V+   N L+   + +R
Sbjct: 504 IYIPLFLPIMVP-VLSSFNMLRGVLQARR 531
>ref|XP_311969.1| ENSANGP00000010967 [Anopheles gambiae]
 gb|EAA07616.1| ENSANGP00000010967 [Anopheles gambiae str. PEST]
          Length = 590

 Score =  430 bits (1094), Expect = e-119
 Identities = 149/616 (24%), Positives = 243/616 (39%), Gaps = 98/616 (15%)

Query: 15  TLSSETQMATMTSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRR-----IWVIVSFWLI 69
           T S ET+  T  SS                  E      S   R      I+  ++F +I
Sbjct: 8   TPSEETKAQTKASSGDMATDKRRVQQDDDASGEQCEAGSSSAAREDAAIHIYATIAFIVI 67

Query: 70  VLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQANSLPE--QEAQNLL 127
           ++++G+P+WWKTT ++R +LP  E+    +        P++ + +     +  +  + L 
Sbjct: 68  IIVIGVPMWWKTTEVYRVSLPYSEIRALNED-------PIKATFRLGLYCQSAERREILS 120

Query: 128 RLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTIRL------LPGDAVTATLD 181
              +   +    F    L LQL S  A      +    L   L        G  +     
Sbjct: 121 FELRKKFEQNFVFE---LDLQLLSLDADTLSTIKTPARLEAELQKHYPSTLGHFLFIEWP 177

Query: 182 PYEPVLDITYPPNAAPSATSASSSLATYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQ 241
             +  + +T    A  S +++S                    + +Y + S+ +  +Y+ +
Sbjct: 178 KLDDDILVTSDRTAFISESASS--------------------LKAYQVLSSWLLQEYKLK 217

Query: 242 SL--TPEAAESLAKRTTRSLKYAP---TYHLTFSLFTSGP--LPSSWDIEAAIQDYLKPV 294
           ++  + +A ++L  R  R L  AP    Y +  ++    P      W+  AA ++Y++P 
Sbjct: 218 AILGSRDAQQTLMGRQLR-LNSAPLQAQYEVLITVLNPRPELQKVHWNARAAAENYIEPF 276

Query: 295 LGLLSPIHNFTIDTQV----QLYASPGVQNQV-----------LSKDDLSSFINAAEWPL 339
           L  LS I NFTI TQ      L  +     QV           L++D L   I + E  L
Sbjct: 277 LSELSGITNFTIKTQWIYQVGLEGAGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKL 336

Query: 340 SPSIGGAPTVNFIVFVGNQ--------------TIALSSE-NAHDSAE---------GGL 375
              I   P ++ +V+V                    L+S   A  SA+            
Sbjct: 337 GTQITDNPCIHLVVYVPPCAQAPLRIYRKDGQRAAPLASNVEAFTSAKWGGIVFANPAEA 396

Query: 376 TTSQSWLIPQWGTVYLLSLPHDTAHVSVDALKQPL-LTFTSHLLSLTGTPSSGSLP--LR 432
           T  +     Q+  VY+ +   D   V +  L++   L   + LL  T  P S   P    
Sbjct: 397 TCVRYMETEQFSDVYIHA--QDVMPVLLYQLRKIFDLENNTPLLDATLVPYSTIEPRAWE 454

Query: 433 LSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVS---KTIVHLRQACETL 489
           + T  R  +  L+  A++TL SL +L   ++ I I   V   +    + IV  +Q     
Sbjct: 455 VDTFMRTNTIYLVHSATTTLQSLIQLLGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAG 514

Query: 490 GEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELK 549
              +    AR A   AERAFF+ SM+  LYFPDE K A+Y+PL  P+ +P+V      LK
Sbjct: 515 RLQQAALLAREAYTSAERAFFDPSMLALLYFPDEQKYAIYIPLFLPIMIPVVFSFNTILK 574

Query: 550 AWRKRKREAAEKGKEK 565
            +RKRK   A K KE+
Sbjct: 575 YFRKRKATTATKAKEE 590
>ref|XP_220633.2| similar to phosphatidylinositol glycan class S [Rattus norvegicus]
          Length = 564

 Score =  423 bits (1076), Expect = e-117
 Identities = 134/551 (24%), Positives = 236/551 (42%), Gaps = 58/551 (10%)

Query: 55  DIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQ 114
           ++ R     + F  + +LLGLP+WWKTT  +RA LP  E+    +    R + P+ +   
Sbjct: 21  EVVRGKRAALFFAAVAILLGLPLWWKTTETYRAPLPYSEISGL-NALQLRLMVPVTVVFT 79

Query: 115 ANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTI-RLLPG 173
            +S+P  + + L     H   +      + ++++     A ++  +  + AL++  +   
Sbjct: 80  RDSVPLDDQEKLPFTVVH---EREIPLKYKMKIKCRFQKAYRRALEHEEEALSLGSVHEA 136

Query: 174 DAVTATLDPYEPVLDITY---PPNAAPSATSAS-------SSLATYVAGQLRSTFAEEQA 223
           +A+ A  +         Y     ++       S       + +   +  +  +       
Sbjct: 137 EALLAEPEKQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAIVRGMIHREAFNIIGRRII 196

Query: 224 IISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSS--W 281
            ++  +S              PE   S  KR  R LK +  Y +TFSL    P      W
Sbjct: 197 HVAQAMSLTEDVLAAALADHLPEDKWSSDKR--RPLKSSLGYEITFSLLNPDPKSHDVHW 254

Query: 282 DIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV--------LSKDDLSSFIN 333
           DIE  +Q Y++P L  LS   NF++D+Q+  YA  GV  +         L+   L   IN
Sbjct: 255 DIEEGVQRYVQPFLNRLSAAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSLPHVIN 314

Query: 334 AAEWPLSPSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYLL 392
             E  L  S     P +NF+++V    +A S     D  +G    + ++  P+WG + + 
Sbjct: 315 PVESRLGSSAASLYPVLNFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGIMVY 371

Query: 393 SLPHDTAHVSVDALK------QPLLTFTSHL--------------LSLTGTPSSGSLPLR 432
           ++     + S   ++      Q +  F + L                L+G  S G +   
Sbjct: 372 NVDPKIYNASELPVRVEVDMAQVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWE 431

Query: 433 LSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEA 492
           L  L   RS + L  A++TL SLA+L   + +I I   VA  V + +  +++A E L   
Sbjct: 432 LDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAEALALG 491

Query: 493 E---GLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG----ML 545
                   ++ A   +ERAFF+ S++  LYFPD+ K A+Y+PL  P+AVP+++      L
Sbjct: 492 HLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFL 551

Query: 546 NELKAWRKRKR 556
              K+W+K ++
Sbjct: 552 ETHKSWKKPEK 562
>gb|AAQ89470.1| PIGS [Homo sapiens]
          Length = 547

 Score =  419 bits (1067), Expect = e-115
 Identities = 133/555 (23%), Positives = 236/555 (41%), Gaps = 60/555 (10%)

Query: 53  LSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRIS 112
            S++ R     + F  + ++LGLP+WWKTT  +RA+LP  ++    +    R + P+ + 
Sbjct: 2   PSEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGL-NALQLRLMVPVTVV 60

Query: 113 IQANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTI-RLL 171
               S+P  + + L     H   +      + ++++     A ++  D  + AL+   + 
Sbjct: 61  FTRESVPLDDQEKLPFTVVH---EREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQ 117

Query: 172 PGDAVTATLDPYEPVLDIT----YPPNAAPSATSAS-------SSLATYVAGQLRSTFAE 220
             +A+       +    +T       ++       S       + +   +  +  +    
Sbjct: 118 EAEAMLDE-PQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGR 176

Query: 221 EQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSS 280
               ++  +S              PE   S  KR  R LK +  Y +TFSL    P    
Sbjct: 177 RIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKR--RPLKSSLGYEITFSLLNPDPKSHD 234

Query: 281 --WDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV--------LSKDDLSS 330
             WDIE A++ Y++P L  L    NF++D+Q+  YA  GV  +         L    L  
Sbjct: 235 VYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPH 294

Query: 331 FINAAEWPLSPSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTV 389
            IN  E  L  S     P +NF+++V    +A S     D  +G    + ++  P+WG +
Sbjct: 295 VINPVESRLGSSAASLYPVLNFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGI 351

Query: 390 YLLSLPHDTAHVSVDALK------QPLLTFTSHL--------------LSLTGTPSSGSL 429
            + ++   T + SV  ++      + +  F + L                L+G  S G +
Sbjct: 352 MVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLM 411

Query: 430 PLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETL 489
              L  L   RS + L  A++TL SLA+L   + +I I   VA  V K +  ++++ E L
Sbjct: 412 TWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEEL 471

Query: 490 GEAE---GLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG--- 543
                      ++ A   +E AFF+ S++  LYFPD+ K A+Y+PL  P+AVP+++    
Sbjct: 472 ASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVK 531

Query: 544 -MLNELKAWRKRKRE 557
             L   K+WRK ++ 
Sbjct: 532 IFLETRKSWRKPEKT 546
>ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapiens]
 sp|Q96S52|PIGS_HUMAN GPI transamidase component PIG-S (Phosphatidylinositol-glycan
           biosynthesis, class S protein)
 dbj|BAB60853.1| phosphatidyl inositol glycan class S [Homo sapiens]
          Length = 555

 Score =  417 bits (1063), Expect = e-115
 Identities = 132/553 (23%), Positives = 235/553 (41%), Gaps = 60/553 (10%)

Query: 55  DIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQ 114
           ++ R     + F  + ++LGLP+WWKTT  +RA+LP  ++    +    R + P+ +   
Sbjct: 12  EVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGL-NALQLRLMVPVTVVFT 70

Query: 115 ANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTI-RLLPG 173
             S+P  + + L     H   +      + ++++     A ++  D  + AL+   +   
Sbjct: 71  RESVPLDDQEKLPFTVVH---EREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQEA 127

Query: 174 DAVTATLDPYEPVLDIT----YPPNAAPSATSAS-------SSLATYVAGQLRSTFAEEQ 222
           +A+       +    +T       ++       S       + +   +  +  +      
Sbjct: 128 EAMLDE-PQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRI 186

Query: 223 AIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSS-- 280
             ++  +S              PE   S  KR  R LK +  Y +TFSL    P      
Sbjct: 187 VQVAQAMSLTEDVLAAALADHLPEDKWSAEKR--RPLKSSLGYEITFSLLNPDPKSHDVY 244

Query: 281 WDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV--------LSKDDLSSFI 332
           WDIE A++ Y++P L  L    NF++D+Q+  YA  GV  +         L    L   I
Sbjct: 245 WDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVI 304

Query: 333 NAAEWPLSPSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYL 391
           N  E  L  S     P +NF+++V    +A S     D  +G    + ++  P+WG + +
Sbjct: 305 NPVESRLGSSAASLYPVLNFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGIMV 361

Query: 392 LSLPHDTAHVSVDALK------QPLLTFTSHL--------------LSLTGTPSSGSLPL 431
            ++   T + SV  ++      + +  F + L                L+G  S G +  
Sbjct: 362 YNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTW 421

Query: 432 RLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGE 491
            L  L   RS + L  A++TL SLA+L   + +I I   VA  V K +  ++++ E L  
Sbjct: 422 ELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELAS 481

Query: 492 AE---GLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG----M 544
                    ++ A   +E AFF+ S++  LYFPD+ K A+Y+PL  P+AVP+++      
Sbjct: 482 GHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIF 541

Query: 545 LNELKAWRKRKRE 557
           L   K+WRK ++ 
Sbjct: 542 LETRKSWRKPEKT 554
>dbj|BAC11615.1| unnamed protein product [Homo sapiens]
          Length = 555

 Score =  410 bits (1045), Expect = e-113
 Identities = 132/553 (23%), Positives = 234/553 (41%), Gaps = 60/553 (10%)

Query: 55  DIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQ 114
           ++ R     + F  + ++LGLP+WWKTT  +RA+LP  ++    +    R + P+ +   
Sbjct: 12  EVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGL-NALQLRLMVPVTVVFT 70

Query: 115 ANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTI-RLLPG 173
             S+P  + + L     H   +      + ++++     A ++  D  + AL+   +   
Sbjct: 71  RESVPLDDQEKLPFTVVH---EREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQEA 127

Query: 174 DAVTATLDPYEPVLDIT----YPPNAAPSATSAS-------SSLATYVAGQLRSTFAEEQ 222
           +A+       +    +T       ++       S       + +   +  +  +      
Sbjct: 128 EAMLDE-PQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRI 186

Query: 223 AIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSS-- 280
             ++  +S              PE   S  KR  R LK +  Y +TFSL    P      
Sbjct: 187 VQVAQAMSLTEDVLAAALADHLPEDKWSAEKR--RPLKSSLGYEITFSLLNPDPKSHDVY 244

Query: 281 WDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV--------LSKDDLSSFI 332
           WDIE A++ Y++P L  L    NF++D+Q+  YA  GV  +         L    L   I
Sbjct: 245 WDIEGAVRRYVQPSLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVI 304

Query: 333 NAAEWPLSPSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYL 391
           N  E  L  S     P +NF+++V    +A S     D  +G    + ++  P+WG + +
Sbjct: 305 NPVESRLGSSAASLYPVLNFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGIMV 361

Query: 392 LSLPHDTAHVSVDALK------QPLLTFTSHL--------------LSLTGTPSSGSLPL 431
            ++   T + SV  ++      + +  F + L                L+G  S G +  
Sbjct: 362 YNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTW 421

Query: 432 RLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGE 491
            L  L   RS + L  A++TL SLA+L   + +I I   VA  V K +  ++++ E L  
Sbjct: 422 ELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELAS 481

Query: 492 AE---GLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG----M 544
                    ++ A   +E AFF+ S++  LYFPD  K A+Y+PL  P+AVP+++      
Sbjct: 482 GHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDYQKFAIYIPLFLPMAVPILLSLVKIF 541

Query: 545 LNELKAWRKRKRE 557
           L   K+WRK ++ 
Sbjct: 542 LETRKSWRKPEKT 554
>ref|XP_203412.1| similar to phosphatidylinositol glycan class S [Mus musculus]
 gb|AAH58979.1| Unknown (protein for MGC:67343) [Mus musculus]
          Length = 555

 Score =  405 bits (1032), Expect = e-111
 Identities = 128/542 (23%), Positives = 235/542 (42%), Gaps = 58/542 (10%)

Query: 64  VSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQANSLPEQEA 123
           + F  + +LLGLP+WWKTT  +RA LP  ++    +    R + P+ +    +S+P  + 
Sbjct: 21  LFFAAVAILLGLPLWWKTTETYRAPLPYSDISGL-NALLLRLMVPVTVVFTRDSVPLDDQ 79

Query: 124 QNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTI-RLLPGDAVTATLDP 182
           + L     H   +      + ++++     A ++  +  + AL++  +   +A+ A  + 
Sbjct: 80  EKLPFTVVH---EREIPLKYKMKIKCRFQKAYRRALEHEEEALSLGSVHEAEAMLAEPEK 136

Query: 183 YEPVLDITY---PPNAAPSATSAS-------SSLATYVAGQLRSTFAEEQAIISYLLSSN 232
                   Y     ++       S       + +   +  +  +        ++  +S  
Sbjct: 137 QAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREAFNIIGRRIVQVAQAMSLT 196

Query: 233 SIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSS--WDIEAAIQDY 290
                       PE   S  KR  R LK +  Y +TFSL    P      WDIE A+Q +
Sbjct: 197 EDVLAAALADHLPEDKWSSDKR--RPLKSSLGYEITFSLLNPDPKSHDVHWDIEGAVQRF 254

Query: 291 LKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV--------LSKDDLSSFINAAEWPLSPS 342
           ++P L  LS   NF++D+Q+  YA  GV  +         L+   L   IN  E  L  S
Sbjct: 255 VQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSLPHVINPVESRLGSS 314

Query: 343 IGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSLPHDTAHV 401
                P ++F+++V    +A S     D  +G    + ++  P+WG + + ++     + 
Sbjct: 315 AASLYPVLHFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGIMVYNVDPKIYNA 371

Query: 402 SVDALK------QPLLTFTSHL--------------LSLTGTPSSGSLPLRLSTLSRIRS 441
           S   ++      + +  F + L                L+G  S G +   L  L   RS
Sbjct: 372 SELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWELDRLLWARS 431

Query: 442 ADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEAE---GLEHA 498
            + L  A++TL SLA+L   + +I I   VA  V + +  +++A + L           +
Sbjct: 432 VENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKALALGHLSSAFAAS 491

Query: 499 RIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLN----ELKAWRKR 554
           + A   +ERAFF+ S++  LYFPD+ K A+Y+PL  P+AVP+++ ++       K+W+K 
Sbjct: 492 QEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFQETRKSWKKP 551

Query: 555 KR 556
           ++
Sbjct: 552 EK 553
>ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Saccharomyces
           cerevisiae]
 sp|Q04080|GP17_YEAST GPI transamidase component GPI17
 pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
 gb|AAB64866.1| Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
          Length = 534

 Score =  380 bits (967), Expect = e-104
 Identities = 130/552 (23%), Positives = 227/552 (40%), Gaps = 75/552 (13%)

Query: 54  SDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISI 113
           S+   R WV   F  I L LG+P+W+K TT++RA+LP+  +    + K       + + +
Sbjct: 2   SNANLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYV 61

Query: 114 QANSLPEQEAQNLLR-LTQHALDDLNDFSGHHLR-LQLSSPAAPQKRNDEPDTALTIRLL 171
           ++++    +  + ++    H L+         L+ L  +      +        +T++L 
Sbjct: 62  KSDTYRFPDVHDAIQVQVNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKLD 121

Query: 172 PGDAVTATLDPYEPVLDITYPPNAAPSATSASSSLATYVAGQLRSTFAEEQAIISYLLSS 231
                ++  D       + Y   A  S       +A  +   +  TF  E   ++     
Sbjct: 122 EFIGYSSAYD--TKETLVYYDDAAVLSNDLPF-FVAQTL---VEHTFQLEWTHLN----- 170

Query: 232 NSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSSWDIEAAIQDYL 291
                         +  E ++     ++ Y P  HL+ +L +    P +W+IE  + DY 
Sbjct: 171 --------------KTCEGVSTNNDVAISYDPNIHLSVTLLSGDGNPVAWEIEPTLTDYF 216

Query: 292 KPVLGLLSPIHNFTIDTQV------QLYASPGVQNQVLSKD-----DLSSFINAAEWP-- 338
            P    LSP+ NFT+D+ +       L++  G    V   D     DLS   + A +P  
Sbjct: 217 SPFRKFLSPLVNFTVDSSIVYHNDLNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPED 276

Query: 339 -------LSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYL 391
                  + PS   +P  + + F+    I         S E       S+L+PQWG + +
Sbjct: 277 SALNLAIVFPSASSSP--DGLAFINGTRI---------SDEITTLDWNSYLVPQWGVIII 325

Query: 392 LSLP-HDTAHVSVDALKQPLLTFTSHLLSLTGTPSSGSLPL-----RLSTLSRIRSADLL 445
             +P    + +S D L+  +  F + +  L G  + GS  L      + +  R+     L
Sbjct: 326 NKMPLKPNSVISEDYLEPMMYRFATDIFQLLGL-TEGSQDLLSPYITIDSFKRLTILQNL 384

Query: 446 LRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEA---------EGLE 496
            +A+ TL SL +L    + +SIP  V+D V + +    Q  + L +            L 
Sbjct: 385 DKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNALH 444

Query: 497 HARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELKAW-RKRK 555
            +       E+AFF   MV Q +FP EH +AVYLPLLGP++  +  G  N +K   +K K
Sbjct: 445 LSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSK 504

Query: 556 REAAEKGKEKKK 567
           +   E+   K+K
Sbjct: 505 KNGTEREVAKEK 516
>ref|NP_187374.2| expressed protein [Arabidopsis thaliana]
 gb|AAL32707.1| Unknown protein [Arabidopsis thaliana]
 gb|AAM91117.1| unknown protein [Arabidopsis thaliana]
          Length = 599

 Score =  351 bits (892), Expect = 3e-95
 Identities = 93/348 (26%), Positives = 144/348 (40%), Gaps = 55/348 (15%)

Query: 258 SLKYAPTYHLTFSLFTSGPLP--SSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYAS 315
            +       L+FSL  S P      WD +   +  L PV   L+PI N T+++QV LY +
Sbjct: 251 PVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQV-LYHT 309

Query: 316 P---------GVQNQVLSKDDLSSFINAAEWPLSPSI---GGAPTVNFIVFVGN---QTI 360
           P          +Q+ +    DL  F+N+ EW L  S    G +  + F+V++ +     +
Sbjct: 310 PKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPL 369

Query: 361 ALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSL--------PHDTAHVSVDALKQPLLT 412
            L   N   S   G      ++ P WG V + +               +S+  L+Q +  
Sbjct: 370 LLQLPNVEISKTNG------FISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEI 423

Query: 413 FTSHLLSLTG-----------------TPSSGSLPLRLSTLSRIRSADLLLRASSTLGSL 455
           F      L G                 T   G     L  LSR  +   L   S+TLGSL
Sbjct: 424 FLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSL 483

Query: 456 ARLALALKSISIPSSVADGVSKTI--VHLRQACETLGEA-EGLEHARIAEAEAERAFFEK 512
           +RL  +L  + I   + + V  ++    L Q+  +LG        +R A + AE AFF  
Sbjct: 484 SRLVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHP 543

Query: 513 SMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELKAWRKRKREAAE 560
           S++   YF  EH  AVY P   PV   +V+  + E   W++ K+E A+
Sbjct: 544 SIMSVSYFSYEHCFAVYSPFFLPVVGHVVLAAVRE---WKRYKQEKAK 588
 Score = 41.3 bits (96), Expect = 0.082
 Identities = 26/99 (26%), Positives = 37/99 (37%), Gaps = 1/99 (1%)

Query: 63  IVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPV-FPLRISIQANSLPEQ 121
           I S  L   LLG+P  WK+  I+R+ LP  ++   +D     P+ FP             
Sbjct: 33  ITSSVLFSFLLGVPFLWKSVEIYRSPLPFHDIDSLSDQLESTPLRFPCNFHAVFVGFRST 92

Query: 122 EAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRND 160
           +  NL    Q  ++ L   S        S     Q R D
Sbjct: 93  DPDNLRSQIQDGINQLTHQSSQCGSCDFSLSVTVQNRED 131
>ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe]
 sp|Q10351|PIGS_SCHPO GPI transamidase component PIG-S homolog
 pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA93813.1| SPAC1F12.09 [Schizosaccharomyces pombe]
          Length = 554

 Score =  343 bits (873), Expect = 5e-93
 Identities = 137/542 (25%), Positives = 233/542 (42%), Gaps = 71/542 (13%)

Query: 35  SSATAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEM 94
           S +   + S  +   PEK      + + ++SF++I+L L +PVWWKTT   R++LP ++M
Sbjct: 52  SDSHEVERSNFQLNKPEK----SLKRYALLSFYVIIL-LAIPVWWKTTHYERSSLPFEDM 106

Query: 95  LDWADGKACRPVFPLRISIQANSLPEQEAQNLLRLTQHALD-DLNDFSGHHLRLQLSSPA 153
            +          F     I      + +  NL +  Q  L+ +   +S +   L+     
Sbjct: 107 ENAPSTVQTHLRFSPTFRI-----LDDKGNNLTKEVQKVLEAEPQIYSYNLKVLE----- 156

Query: 154 APQKRNDEPDTALTIRLLPGDAVTATLDPYEPVLDITYPPNAAPSATSASSSLATYVAGQ 213
                  +      I L     +    D    ++D         + +   S LA  +   
Sbjct: 157 -------DDPVDYRIVLRESTDLQWFWDENNFIID---------TPSKGPSELAILIVNC 200

Query: 214 LRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFT 273
           L   F       S  +            ++ P  AE     T R+++++P Y +  SL  
Sbjct: 201 LWEAF-------SPQVMEVWSKFTRFSSTVEPSRAE-----TKRTVQFSPQYRVLLSLLV 248

Query: 274 --SGPLPSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQVLSKD----- 326
                 P +WDIE AIQ Y  P++  L+ +    I+TQ+Q +       +   KD     
Sbjct: 249 GEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVEDA---EAYIKDDKFCT 305

Query: 327 ---DLSSFINAAEW--PLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSW 381
              DL + +N  E     SP I   PT++F+++V +  I        DS    +  + S 
Sbjct: 306 KHADLPNLVNNFEKYLSFSPHI-REPTIHFVLYVPSPQIQPLWLENEDSN---IIPTNSM 361

Query: 382 LIPQWGTVYLLSLPHDTAHVSVD-ALKQPLLTFTSHLLSLTG---TPSSGSLPLRLSTLS 437
           L+PQWG++  ++       +  D  LK      +  LL L G    P S         L 
Sbjct: 362 LLPQWGSITTINFNVTEKKLLHDVDLKDYFRVISRDLLLLLGINDVPVSSLSATLADRLL 421

Query: 438 RIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGE---AEG 494
           R R A+  + AS TL +LA+L  +++S+++P  +   V  T++ L  A + L +    E 
Sbjct: 422 RQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLNEA 481

Query: 495 LEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELK-AWRK 553
           L ++  A ++++ A F  SMV  +YFPDE K  +Y PL  P+ +PL++  + E+K   R+
Sbjct: 482 LSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISFIKEVKDMLRE 541

Query: 554 RK 555
           RK
Sbjct: 542 RK 543
>gb|AAF20228.1| unknown protein [Arabidopsis thaliana]
          Length = 605

 Score =  314 bits (797), Expect = 5e-84
 Identities = 84/317 (26%), Positives = 128/317 (39%), Gaps = 52/317 (16%)

Query: 258 SLKYAPTYHLTFSLFTSGPLP--SSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYAS 315
            +       L+FSL  S P      WD +   +  L PV   L+PI N T+++QV LY +
Sbjct: 274 PVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQV-LYHT 332

Query: 316 P---------GVQNQVLSKDDLSSFINAAEWPLSPSI---GGAPTVNFIVFVGN---QTI 360
           P          +Q+ +    DL  F+N+ EW L  S    G +  + F+V++ +     +
Sbjct: 333 PKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPL 392

Query: 361 ALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSL--------PHDTAHVSVDALKQPLLT 412
            L   N   S   G      ++ P WG V + +               +S+  L+Q +  
Sbjct: 393 LLQLPNVEISKTNG------FISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEI 446

Query: 413 FTSHLLSLTG-----------------TPSSGSLPLRLSTLSRIRSADLLLRASSTLGSL 455
           F      L G                 T   G     L  LSR  +   L   S+TLGSL
Sbjct: 447 FLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSL 506

Query: 456 ARLALALKSISIPSSVADGVSKTI--VHLRQACETLGEA-EGLEHARIAEAEAERAFFEK 512
           +RL  +L  + I   + + V  ++    L Q+  +LG        +R A + AE AFF  
Sbjct: 507 SRLVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHP 566

Query: 513 SMVGQLYFPDEHKVAVY 529
           S++   YF  EH  AVY
Sbjct: 567 SIMSVSYFSYEHCFAVY 583
 Score = 37.8 bits (87), Expect = 0.81
 Identities = 21/88 (23%), Positives = 32/88 (35%), Gaps = 1/88 (1%)

Query: 74  GLPVWWKTTTIHRANLPLKEMLDWADGKACRPV-FPLRISIQANSLPEQEAQNLLRLTQH 132
           G+P  WK+  I+R+ LP  ++   +D     P+ FP             +  NL    Q 
Sbjct: 67  GVPFLWKSVEIYRSPLPFHDIDSLSDQLESTPLRFPCNFHAVFVGFRSTDPDNLRSQIQD 126

Query: 133 ALDDLNDFSGHHLRLQLSSPAAPQKRND 160
            ++ L   S        S     Q R D
Sbjct: 127 GINQLTHQSSQCGSCDFSLSVTVQNRED 154
>gb|AAH01319.2| PIGS protein [Homo sapiens]
          Length = 243

 Score =  255 bits (646), Expect = 2e-66
 Identities = 66/245 (26%), Positives = 115/245 (46%), Gaps = 31/245 (12%)

Query: 341 PSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSLPHDTA 399
            S     P +NF+++V    +A S     D  +G    + ++  P+WG + + ++   T 
Sbjct: 1   SSAASLYPVLNFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGIMVYNVDSKTY 57

Query: 400 HVSVDALK------QPLLTFTSHL--------------LSLTGTPSSGSLPLRLSTLSRI 439
           + SV  ++      + +  F + L                L+G  S G +   L  L   
Sbjct: 58  NASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWA 117

Query: 440 RSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEAE---GLE 496
           RS + L  A++TL SLA+L   + +I I   VA  V K +  ++++ E L          
Sbjct: 118 RSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFV 177

Query: 497 HARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG----MLNELKAWR 552
            ++ A   +E AFF+ S++  LYFPD+ K A+Y+PL  P+AVP+++      L   K+WR
Sbjct: 178 ASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRKSWR 237

Query: 553 KRKRE 557
           K ++ 
Sbjct: 238 KPEKT 242
>dbj|BAB92563.1| P0497A05.6 [Oryza sativa (japonica cultivar-group)]
          Length = 557

 Score =  244 bits (618), Expect = 5e-63
 Identities = 73/310 (23%), Positives = 117/310 (37%), Gaps = 43/310 (13%)

Query: 203 SSSLATYVAGQLRSTFAEEQAIISYLLS-SNSIPTDYRPQSLTPEAAESLAKRTTRSLKY 261
           S  L   V G+ R  +   +   + ++S    +   Y       E   S  KR    +  
Sbjct: 210 SDDLKRIVIGKHRHAWVVGKVDEAEVVSIIGKVFVKYFMNGGVEEGEASTVKREFMPVGS 269

Query: 262 APTYHLTFSLFTSGPLP--SSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQL------- 312
                L+FSL  + P      W+ E   Q  L PV+  L PI N  I++QV         
Sbjct: 270 DGNIVLSFSLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQVLYHTPKSSY 329

Query: 313 -YASPGVQNQVLSKDDLSSFINAAEWPLSPSI---GGAPTVNFIVFVGNQTIALSSENAH 368
            Y+   +   VLS  D+  F+N+ EW L  SI   G +  + F+V++ +   A       
Sbjct: 330 SYSDDKLGGNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYIPS---ARECPLYL 386

Query: 369 DSAEGGLTTSQSWLIPQWGTVYLLSLP-------HDTA--HVSVDALKQPLLTFTSHLLS 419
              +G L+ + +++ P WG V + + P          A   +S + L + +  F   L  
Sbjct: 387 QLPDGELSKTNAFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSEELMETIEIFIGQLRQ 446

Query: 420 LTGTPSS-----------------GSLPLRLSTLSRIRSADLLLRASSTLGSLARLALAL 462
           L G  SS                 G     L  L R  +   LL   +TL SL+ L  +L
Sbjct: 447 LFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLLYRHHACSNLLSCLTTLESLSSLVQSL 506

Query: 463 KSISIPSSVA 472
             + +   + 
Sbjct: 507 PRMIVMDEIG 516
 Score = 38.2 bits (88), Expect = 0.63
 Identities = 24/91 (26%), Positives = 38/91 (41%), Gaps = 12/91 (13%)

Query: 19  ETQMATMTSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVW 78
           E   A  TSSP            +    +P  ++L        V+ +  L+  L+GLP+ 
Sbjct: 23  EASGAPSTSSPSDA---DGGGKPSPRTSKPGRKRL--------VLTASVLLSFLIGLPLL 71

Query: 79  WKTTTIHRANLPLKEMLDWADG-KACRPVFP 108
            K+T IHR+ LP   +   +    +  P FP
Sbjct: 72  LKSTEIHRSPLPSDAIAALSRRLHSNPPSFP 102
>ref|XP_142436.3| similar to phosphatidylinositol glycan class S [Mus musculus]
          Length = 225

 Score =  195 bits (493), Expect = 2e-48
 Identities = 50/193 (25%), Positives = 80/193 (40%), Gaps = 16/193 (8%)

Query: 209 YVAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLT 268
            +  +  +        ++  +S              PE   S  KR  R LK +  Y +T
Sbjct: 34  LIHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKR--RPLKSSLGYEIT 91

Query: 269 FSLFTSGPLPSS--WDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV---- 322
           FSL    P      WDIE A+Q +++P L  LS   NF++D+Q+  YA  GV  +     
Sbjct: 92  FSLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPAS 151

Query: 323 ----LSKDDLSSFINAAEWPLSPSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTT 377
               L+   L   IN  E  L  S     P ++F+++V    +A S     D  +G    
Sbjct: 152 SSYSLAMHSLPHVINPVESRLGSSAASLYPVLHFLLYVP--ELAHSPLYIQD-KDGAPVA 208

Query: 378 SQSWLIPQWGTVY 390
           + ++  P+WG + 
Sbjct: 209 TNAFHSPRWGGIM 221
>dbj|BAB70938.1| unnamed protein product [Homo sapiens]
          Length = 184

 Score =  188 bits (475), Expect = 3e-46
 Identities = 56/180 (31%), Positives = 88/180 (48%), Gaps = 24/180 (13%)

Query: 400 HVSVDALKQPLLTFTSHL--------------LSLTGTPSSGSLPLRLSTLSRIRSADLL 445
            V VD ++  +  F + L                L+G  S G +   L  L   RS + L
Sbjct: 6   RVEVDMVR-VMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENL 64

Query: 446 LRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEAEGLEHARIAEAE- 504
             A++TL SLA+L   + +I I   VA  V K +  ++++ E L     L  A +A  E 
Sbjct: 65  ATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGH-LASAFVASQEV 123

Query: 505 ---AERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG----MLNELKAWRKRKRE 557
              +E AFF+ S++  LYFPD+ K  +Y+PL  P+AVP+++      L   K+WRK ++ 
Sbjct: 124 VTSSELAFFDPSLLHLLYFPDDQKFPIYIPLFLPMAVPILLSLVKLFLETRKSWRKPEKT 183
>emb|CAE45912.1| hypothetical protein [Homo sapiens]
          Length = 162

 Score =  182 bits (459), Expect = 2e-44
 Identities = 48/147 (32%), Positives = 77/147 (51%), Gaps = 7/147 (4%)

Query: 418 LSLTGTPSSGSLPLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSK 477
             L+G  S G +   L  L   RS + L  A++TL SLA+L   + +I I   VA  V K
Sbjct: 15  CLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYK 74

Query: 478 TIVHLRQACETLGEAE---GLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLG 534
            +  ++++ E L           ++ A   +E  FF+ S++  LYFPD+ K A+Y+PL  
Sbjct: 75  AVAAVQKSAEELASGHLASAFVASQEAVTSSELVFFDPSLLHLLYFPDDQKFAIYIPLFL 134

Query: 535 PVAVPLVMG----MLNELKAWRKRKRE 557
           P+AVP+++      L   K+WRK ++ 
Sbjct: 135 PMAVPILLSLVKIFLETRKSWRKPEKT 161
>ref|NP_501852.1| phosphatidylinositol glycan class S like (4K912) [Caenorhabditis
           elegans]
 pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
 emb|CAA93093.1| Hypothetical protein T14G10.7 [Caenorhabditis elegans]
          Length = 544

 Score =  125 bits (312), Expect = 4e-27
 Identities = 33/114 (28%), Positives = 61/114 (52%), Gaps = 7/114 (6%)

Query: 430 PLRLSTLSRIR-SADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACET 488
              ++  +R+R S D  +RA+S + +L +LA  + +I I   VAD  ++ +  L    + 
Sbjct: 418 QWEVAR-ARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVADRATRAVEIL---NDV 473

Query: 489 LGEAEGLEHARIAEAE--AERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPL 540
           +     L+  R+ EA   A+ A  + S++  LYFP + + AV +PL  P+ +P+
Sbjct: 474 VRPGRMLDFERLLEARRLADSANSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPI 527
 Score = 40.1 bits (93), Expect = 0.16
 Identities = 12/37 (32%), Positives = 24/37 (64%)

Query: 54 SDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLP 90
          S+   ++   +SF ++V  +G+P+W  TT+ +R+N P
Sbjct: 40 SERPYKLAASLSFVILVFFIGMPMWHFTTSTYRSNFP 76
CPU time:   197.08 user secs.	    6.00 sys. secs	  203.08 total secs.

  Database: nr
    Posted date:  Nov 22, 2003  1:30 PM
  Number of letters in database: 504,497,942
  Number of sequences in database:  1,543,518
  
Lambda     K      H
   0.306    0.160    0.441 

Gapped
Lambda     K      H
   0.270   0.0561    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 523387523
Number of Sequences: 1543518
Number of extensions: 21970585
Number of successful extensions: 113040
Number of sequences better than 10.0: 87
Number of HSP's better than 10.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 65
Number of HSP's that attempted gapping in prelim test: 112706
Number of HSP's gapped (non-prelim): 240
length of query: 568
length of database: 504,497,942
effective HSP length: 63
effective length of query: 505
effective length of database: 407,256,308
effective search space: 205664435540
effective search space used: 205664435540
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (20.7 bits)
S2: 78 (34.5 bits)