Sequences with E-value BETTER than threshold (0.002)
ref|XP_325888.1| hypothetical protein [Neurospora crassa] >gi|28... 828 0.0
emb|CAD60556.1| unnamed protein product [Podospora anserina] 722 0.0
gb|EAA57290.1| hypothetical protein MG08259.4 [Magnaporthe grise... 700 0.0
gb|AAO39601.1| GM10512p [Drosophila melanogaster] 587 e-166
ref|NP_733060.1| CG31120-PB [Drosophila melanogaster] >gi|169020... 584 e-165
ref|XP_311969.1| ENSANGP00000010967 [Anopheles gambiae] >gi|2129... 430 e-119
ref|XP_220633.2| similar to phosphatidylinositol glycan class S ... 423 e-117
gb|AAQ89470.1| PIGS [Homo sapiens] 419 e-115
ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapie... 417 e-115
dbj|BAC11615.1| unnamed protein product [Homo sapiens] 410 e-113
ref|XP_203412.1| similar to phosphatidylinositol glycan class S ... 405 e-111
ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Sacc... 380 e-104
ref|NP_187374.2| expressed protein [Arabidopsis thaliana] >gi|17... 351 3e-95
ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe... 343 5e-93
gb|AAF20228.1| unknown protein [Arabidopsis thaliana] 314 5e-84
gb|AAH01319.2| PIGS protein [Homo sapiens] 255 2e-66
dbj|BAB92563.1| P0497A05.6 [Oryza sativa (japonica cultivar-group)] 244 5e-63
ref|XP_142436.3| similar to phosphatidylinositol glycan class S ... 195 2e-48
dbj|BAB70938.1| unnamed protein product [Homo sapiens] 188 3e-46
emb|CAE45912.1| hypothetical protein [Homo sapiens] 182 2e-44
ref|NP_501852.1| phosphatidylinositol glycan class S like (4K912... 125 4e-27
Alignments
>ref|XP_325888.1| hypothetical protein [Neurospora crassa]
gb|EAA30387.1| hypothetical protein [Neurospora crassa]
Length = 568
Score = 828 bits (2116), Expect = 0.0
Identities = 556/556 (100%), Positives = 556/556 (100%)
Query: 1 MFARRPIDHGAAGDTLSSETQMATMTSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRRI 60
MFARRPIDHGAAGDTLSSETQMATMTSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRRI
Sbjct: 1 MFARRPIDHGAAGDTLSSETQMATMTSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRRI 60
Query: 61 WVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQANSLPE 120
WVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQANSLPE
Sbjct: 61 WVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQANSLPE 120
Query: 121 QEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTIRLLPGDAVTATL 180
QEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTIRLLPGDAVTATL
Sbjct: 121 QEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTIRLLPGDAVTATL 180
Query: 181 DPYEPVLDITYPPNAAPSATSASSSLATYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRP 240
DPYEPVLDITYPPNAAPSATSASSSLATYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRP
Sbjct: 181 DPYEPVLDITYPPNAAPSATSASSSLATYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRP 240
Query: 241 QSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSSWDIEAAIQDYLKPVLGLLSP 300
QSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSSWDIEAAIQDYLKPVLGLLSP
Sbjct: 241 QSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSSWDIEAAIQDYLKPVLGLLSP 300
Query: 301 IHNFTIDTQVQLYASPGVQNQVLSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTI 360
IHNFTIDTQVQLYASPGVQNQVLSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTI
Sbjct: 301 IHNFTIDTQVQLYASPGVQNQVLSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTI 360
Query: 361 ALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSLPHDTAHVSVDALKQPLLTFTSHLLSL 420
ALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSLPHDTAHVSVDALKQPLLTFTSHLLSL
Sbjct: 361 ALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSLPHDTAHVSVDALKQPLLTFTSHLLSL 420
Query: 421 TGTPSSGSLPLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIV 480
TGTPSSGSLPLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIV
Sbjct: 421 TGTPSSGSLPLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIV 480
Query: 481 HLRQACETLGEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPL 540
HLRQACETLGEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPL
Sbjct: 481 HLRQACETLGEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPL 540
Query: 541 VMGMLNELKAWRKRKR 556
VMGMLNELKAWRKRKR
Sbjct: 541 VMGMLNELKAWRKRKR 556
>emb|CAD60556.1| unnamed protein product [Podospora anserina]
Length = 542
Score = 722 bits (1846), Expect = 0.0
Identities = 417/537 (77%), Positives = 469/537 (86%), Gaps = 14/537 (2%)
Query: 32 TPLSSATAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPL 91
++ A TS++K PPPEK SD+RRR VI+SFWLIVLLLGLP+WWKTTTI+RA+LPL
Sbjct: 16 ATIAPKQEAPTSSKKLPPPEKPSDVRRRSHVILSFWLIVLLLGLPIWWKTTTIYRADLPL 75
Query: 92 KEMLDWADGKACRPVFPLRISIQANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSS 151
++ML+WADGKACRPVFPLRISIQA SL +QEAQNLLRLTQHALDDLNDF+GHHLRLQ+
Sbjct: 76 EDMLEWADGKACRPVFPLRISIQATSLQDQEAQNLLRLTQHALDDLNDFAGHHLRLQMGD 135
Query: 152 PAAPQKRNDEPD--TALTIRLLPGDAVTATLDPYEPVLDITYPPNAAPSATSASSSLATY 209
++ D D +ALTIRL PG+ TATLDPYEP+LDITYP N PS T++SS+LATY
Sbjct: 136 -----EKQDSADNGSALTIRLTPGEGTTATLDPYEPILDITYPQNTIPSPTASSSALATY 190
Query: 210 VAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTF 269
+A QLR+TFAEEQA ISYLLS+NS+P+++RP L+PE AE+LAKRTTRSLKYAPTYHLTF
Sbjct: 191 IASQLRTTFAEEQATISYLLSTNSMPSEHRPLGLSPETAEALAKRTTRSLKYAPTYHLTF 250
Query: 270 SLFTSGPLPSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQVLSKDDLS 329
SLFTSGPLPSSWDIE AI++Y+KPVL +LSPIHNFTIDTQVQLYASPGVQNQVL+K+DLS
Sbjct: 251 SLFTSGPLPSSWDIETAIEEYMKPVLDVLSPIHNFTIDTQVQLYASPGVQNQVLNKEDLS 310
Query: 330 SFINAAEWPLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTV 389
SFINAAEWPLSPSIGGAPT+NFIVFVGNQTIA S+ A TS SWLIPQWGTV
Sbjct: 311 SFINAAEWPLSPSIGGAPTINFIVFVGNQTIASPSQEA-------APTSHSWLIPQWGTV 363
Query: 390 YLLSLPHDTAHVSVDALKQPLLTFTSHLLSLTGTPSSGSLPLRLSTLSRIRSADLLLRAS 449
+LLSLP DT HV+ LKQPLLTFTSHLLSLTGTP SGSLPLRLSTL+RIRSADLLLRAS
Sbjct: 364 FLLSLPGDTTHVAATTLKQPLLTFTSHLLSLTGTPESGSLPLRLSTLARIRSADLLLRAS 423
Query: 450 STLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEAEGLEHARIAEAEAERAF 509
STLGSLARLA+AL SISIPSSVADGV+KT+ HLR AC+ LG EGL HARIAE EAERAF
Sbjct: 424 STLGSLARLAMALPSISIPSSVADGVTKTMSHLRLACDNLGGTEGLAHARIAEVEAERAF 483
Query: 510 FEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELKAWRKRKREAAEKGKEKK 566
FEKSMVGQLYFPDEHK+AVYLPLLGPVAVPLVMG++NE+KAWRKR+RE AEKG EKK
Sbjct: 484 FEKSMVGQLYFPDEHKIAVYLPLLGPVAVPLVMGLINEIKAWRKRRRERAEKGAEKK 540
>gb|EAA57290.1| hypothetical protein MG08259.4 [Magnaporthe grisea 70-15]
Length = 558
Score = 700 bits (1788), Expect = 0.0
Identities = 322/544 (59%), Positives = 406/544 (74%), Gaps = 16/544 (2%)
Query: 26 TSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIH 85
+ + VA PL + T K+PPPEK +D+RRR +VI+SFW ++L +GLP+WW TT+++
Sbjct: 27 SGARVAAPLPA-----NKTNKDPPPEKATDVRRRSYVILSFWALILSVGLPIWWHTTSVY 81
Query: 86 RANLPLKEMLDWADGKACRPVFPLRISIQANSLPEQEAQNLLRLTQHALDDLNDFSGHHL 145
RA+LPL EM+DWADG+ACRP FPL I+I+ ++ + E L+++TQ LD L++ +
Sbjct: 82 RASLPLDEMMDWADGRACRPTFPLTIAIEKGNMRQDETDVLIQMTQMVLDSLSESTDSTD 141
Query: 146 RLQLSSPAAPQKRNDEPDTALTIRLLPGDAVTATLDPYEPVLDITYPPNAAPSATSASSS 205
R QL A + D+ ALT+RL PGD T L P +P+L +TY N P S
Sbjct: 142 R-QLRLTVADETSVDDEKIALTVRLNPGDTTTTALQPEQPILQVTYAANTLP-----SPG 195
Query: 206 LATYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTY 265
LA+ +A + + +EEQA++S L++ S T ++P A ++AKR+TRSLKYAPTY
Sbjct: 196 LASRIARDVHAVLSEEQAVVSNLVAEASGSTKPSLSGVSPAVAAAVAKRSTRSLKYAPTY 255
Query: 266 HLTFSLFTSGPLPSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQVLSK 325
HLTFSLFT+G PSSWDI+AA++ Y++PVL +LSPIHNFTIDTQVQLYA+PGVQ+ VLSK
Sbjct: 256 HLTFSLFTAGSSPSSWDIDAAVEQYMRPVLDVLSPIHNFTIDTQVQLYAAPGVQSDVLSK 315
Query: 326 DDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQ 385
DDLSSFINAAEWPLSPSIGGAPT+NF+VFVGNQ+I+L + A A T+QSWLIPQ
Sbjct: 316 DDLSSFINAAEWPLSPSIGGAPTINFVVFVGNQSISLPTTEAGSDATA---TAQSWLIPQ 372
Query: 386 WGTVYLLSLPHDT-AHVSVDALKQPLLTFTSHLLSLTGTP-SSGSLPLRLSTLSRIRSAD 443
WGTVYL+ + V +L+ P+ TFT+HLLSL GTP +SGSLPLRL+ L RIRSAD
Sbjct: 373 WGTVYLVPQTAENANKVPAQSLRAPMATFTAHLLSLLGTPETSGSLPLRLAGLRRIRSAD 432
Query: 444 LLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEAEGLEHARIAEA 503
L+LRASS+LGSLARL+ AL I+IP SVADGVS TI HLRQAC+ LG + GL HARIA+
Sbjct: 433 LVLRASSSLGSLARLSRALPGIAIPRSVADGVSTTIRHLRQACDALGTSAGLAHARIAQV 492
Query: 504 EAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELKAWRKRKREAAEKGK 563
A++AFFEKSMVGQLYFPDEHKVAVYLPLLGPV VPLVMG+LNELKAWRKR++E A K
Sbjct: 493 AADKAFFEKSMVGQLYFPDEHKVAVYLPLLGPVGVPLVMGLLNELKAWRKRRQEKAAAEK 552
Query: 564 EKKK 567
KK
Sbjct: 553 TGKK 556
>gb|AAO39601.1| GM10512p [Drosophila melanogaster]
Length = 559
Score = 587 bits (1497), Expect = e-166
Identities = 129/571 (22%), Positives = 240/571 (41%), Gaps = 88/571 (15%)
Query: 36 SATAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEML 95
S +S+ +P + + + RI ++F ++++ +G+P+WW+TTT++R NLP E+L
Sbjct: 22 SIMETSSSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEIL 81
Query: 96 DWADGKACRPVFPLRISIQANSLPEQEA--QNLLRLTQHALDDLNDFSGHHLRLQLSSPA 153
+ P++ ++Q +Q + Q L+ Q+A D +S +L
Sbjct: 82 SLSK-------IPIKTAVQVAIYTQQTSRGQLLIAELQNAFSDNEIWSVEFKQLS----P 130
Query: 154 APQKRNDEPDTALTIRLLP------GDAVTATLDPYEPVLDITYPPNAAPSATSASSSLA 207
P + AL LL GD + + L +T +A + + S+ +A
Sbjct: 131 TPMTQEAHTPAALEKLLLENHVQSVGDFMFIEWPKLQEELLLTTERSALMRSDTPSNKIA 190
Query: 208 TYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHL 267
+ ++ T+ I+ +LS++ + +S P Y +
Sbjct: 191 QLLHAKILQTYR-----INQILSTDE-------------------RMGAKSEAPQPAYDV 226
Query: 268 TFSLFTSGPL--PSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYAS-----PGVQN 320
S+ P + W+I A++ Y++P L +S + N+T+ +Q + + V++
Sbjct: 227 IVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVRD 286
Query: 321 Q-------VLSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEG 373
Q L + L + + LS S P +N +V++ IA + ++S +
Sbjct: 287 QSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCHIA--PLHIYNSKDQ 344
Query: 374 GLTTS--QSWLIPQWGTVYLLSLPHDTA------------HVSVDALKQPLLTFTSHLLS 419
LT + +++ P WG + + P HVS Q +L LL
Sbjct: 345 RLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLD 404
Query: 420 LTGTPSSGSL---------PLRLSTLSRIRSADL--LLRASSTLGSLARLALALKSISIP 468
++ + P R + +R + + + ASSTL SL +L + I I
Sbjct: 405 ISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSLIKLLDQISYIVID 464
Query: 469 SSVADGVSKTIVHLRQACETL---GEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHK 525
V ++ + + A L A+ A+ A +ER FF+ S++ QLYFPDE K
Sbjct: 465 DEVGTAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGFFDASLLAQLYFPDEQK 524
Query: 526 VAVYLPLLGPVAVPLVMGMLNELKAWRKRKR 556
A+Y+PL P+ VP V+ N L+ + +R
Sbjct: 525 YAIYIPLFLPIMVP-VLSSFNMLRGVLQARR 554
>ref|NP_733060.1| CG31120-PB [Drosophila melanogaster]
gb|AAL27645.1| SD10476p [Drosophila melanogaster]
gb|AAF56366.2| CG31120-PB [Drosophila melanogaster]
Length = 536
Score = 584 bits (1491), Expect = e-165
Identities = 128/569 (22%), Positives = 239/569 (41%), Gaps = 88/569 (15%)
Query: 38 TAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDW 97
+S+ +P + + + RI ++F ++++ +G+P+WW+TTT++R NLP E+L
Sbjct: 1 METSSSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSL 60
Query: 98 ADGKACRPVFPLRISIQANSLPEQEA--QNLLRLTQHALDDLNDFSGHHLRLQLSSPAAP 155
+ P++ ++Q +Q + Q L+ Q+A D +S +L P
Sbjct: 61 SK-------IPIKTAVQVAIYTQQTSRGQLLIAELQNAFSDNEIWSVEFKQLS----PTP 109
Query: 156 QKRNDEPDTALTIRLLP------GDAVTATLDPYEPVLDITYPPNAAPSATSASSSLATY 209
+ AL LL GD + + L +T +A + + S+ +A
Sbjct: 110 MTQEAHTPAALEKLLLENHVQSVGDFMFIEWPKLQEELLLTTERSALMRSDTPSNKIAQL 169
Query: 210 VAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTF 269
+ ++ T+ I+ +LS++ + +S P Y +
Sbjct: 170 LHAKILQTYR-----INQILSTDE-------------------RMGAKSEAPQPAYDVIV 205
Query: 270 SLFTSGPL--PSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYAS-----PGVQNQ- 321
S+ P + W+I A++ Y++P L +S + N+T+ +Q + + V++Q
Sbjct: 206 SVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVRDQS 265
Query: 322 ------VLSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGL 375
L + L + + LS S P +N +V++ IA + ++S + L
Sbjct: 266 KLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCHIA--PLHIYNSKDQRL 323
Query: 376 TTS--QSWLIPQWGTVYLLSLPHDTA------------HVSVDALKQPLLTFTSHLLSLT 421
T + +++ P WG + + P HVS Q +L LL ++
Sbjct: 324 TRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLDIS 383
Query: 422 GTPSSGSL---------PLRLSTLSRIRSADL--LLRASSTLGSLARLALALKSISIPSS 470
+ P R + +R + + + ASSTL SL +L + I I
Sbjct: 384 SELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSLIKLLDQISYIVIDDE 443
Query: 471 VADGVSKTIVHLRQACETL---GEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVA 527
V ++ + + A L A+ A+ A +ER FF+ S++ QLYFPDE K A
Sbjct: 444 VGAAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGFFDASLLAQLYFPDEQKYA 503
Query: 528 VYLPLLGPVAVPLVMGMLNELKAWRKRKR 556
+Y+PL P+ VP V+ N L+ + +R
Sbjct: 504 IYIPLFLPIMVP-VLSSFNMLRGVLQARR 531
>ref|XP_311969.1| ENSANGP00000010967 [Anopheles gambiae]
gb|EAA07616.1| ENSANGP00000010967 [Anopheles gambiae str. PEST]
Length = 590
Score = 430 bits (1094), Expect = e-119
Identities = 149/616 (24%), Positives = 243/616 (39%), Gaps = 98/616 (15%)
Query: 15 TLSSETQMATMTSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRR-----IWVIVSFWLI 69
T S ET+ T SS E S R I+ ++F +I
Sbjct: 8 TPSEETKAQTKASSGDMATDKRRVQQDDDASGEQCEAGSSSAAREDAAIHIYATIAFIVI 67
Query: 70 VLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQANSLPE--QEAQNLL 127
++++G+P+WWKTT ++R +LP E+ + P++ + + + + + L
Sbjct: 68 IIVIGVPMWWKTTEVYRVSLPYSEIRALNED-------PIKATFRLGLYCQSAERREILS 120
Query: 128 RLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTIRL------LPGDAVTATLD 181
+ + F L LQL S A + L L G +
Sbjct: 121 FELRKKFEQNFVFE---LDLQLLSLDADTLSTIKTPARLEAELQKHYPSTLGHFLFIEWP 177
Query: 182 PYEPVLDITYPPNAAPSATSASSSLATYVAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQ 241
+ + +T A S +++S + +Y + S+ + +Y+ +
Sbjct: 178 KLDDDILVTSDRTAFISESASS--------------------LKAYQVLSSWLLQEYKLK 217
Query: 242 SL--TPEAAESLAKRTTRSLKYAP---TYHLTFSLFTSGP--LPSSWDIEAAIQDYLKPV 294
++ + +A ++L R R L AP Y + ++ P W+ AA ++Y++P
Sbjct: 218 AILGSRDAQQTLMGRQLR-LNSAPLQAQYEVLITVLNPRPELQKVHWNARAAAENYIEPF 276
Query: 295 LGLLSPIHNFTIDTQV----QLYASPGVQNQV-----------LSKDDLSSFINAAEWPL 339
L LS I NFTI TQ L + QV L++D L I + E L
Sbjct: 277 LSELSGITNFTIKTQWIYQVGLEGAGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKL 336
Query: 340 SPSIGGAPTVNFIVFVGNQ--------------TIALSSE-NAHDSAE---------GGL 375
I P ++ +V+V L+S A SA+
Sbjct: 337 GTQITDNPCIHLVVYVPPCAQAPLRIYRKDGQRAAPLASNVEAFTSAKWGGIVFANPAEA 396
Query: 376 TTSQSWLIPQWGTVYLLSLPHDTAHVSVDALKQPL-LTFTSHLLSLTGTPSSGSLP--LR 432
T + Q+ VY+ + D V + L++ L + LL T P S P
Sbjct: 397 TCVRYMETEQFSDVYIHA--QDVMPVLLYQLRKIFDLENNTPLLDATLVPYSTIEPRAWE 454
Query: 433 LSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVS---KTIVHLRQACETL 489
+ T R + L+ A++TL SL +L ++ I I V + + IV +Q
Sbjct: 455 VDTFMRTNTIYLVHSATTTLQSLIQLLGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAG 514
Query: 490 GEAEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELK 549
+ AR A AERAFF+ SM+ LYFPDE K A+Y+PL P+ +P+V LK
Sbjct: 515 RLQQAALLAREAYTSAERAFFDPSMLALLYFPDEQKYAIYIPLFLPIMIPVVFSFNTILK 574
Query: 550 AWRKRKREAAEKGKEK 565
+RKRK A K KE+
Sbjct: 575 YFRKRKATTATKAKEE 590
>ref|XP_220633.2| similar to phosphatidylinositol glycan class S [Rattus norvegicus]
Length = 564
Score = 423 bits (1076), Expect = e-117
Identities = 134/551 (24%), Positives = 236/551 (42%), Gaps = 58/551 (10%)
Query: 55 DIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQ 114
++ R + F + +LLGLP+WWKTT +RA LP E+ + R + P+ +
Sbjct: 21 EVVRGKRAALFFAAVAILLGLPLWWKTTETYRAPLPYSEISGL-NALQLRLMVPVTVVFT 79
Query: 115 ANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTI-RLLPG 173
+S+P + + L H + + ++++ A ++ + + AL++ +
Sbjct: 80 RDSVPLDDQEKLPFTVVH---EREIPLKYKMKIKCRFQKAYRRALEHEEEALSLGSVHEA 136
Query: 174 DAVTATLDPYEPVLDITY---PPNAAPSATSAS-------SSLATYVAGQLRSTFAEEQA 223
+A+ A + Y ++ S + + + + +
Sbjct: 137 EALLAEPEKQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAIVRGMIHREAFNIIGRRII 196
Query: 224 IISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSS--W 281
++ +S PE S KR R LK + Y +TFSL P W
Sbjct: 197 HVAQAMSLTEDVLAAALADHLPEDKWSSDKR--RPLKSSLGYEITFSLLNPDPKSHDVHW 254
Query: 282 DIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV--------LSKDDLSSFIN 333
DIE +Q Y++P L LS NF++D+Q+ YA GV + L+ L IN
Sbjct: 255 DIEEGVQRYVQPFLNRLSAAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSLPHVIN 314
Query: 334 AAEWPLSPSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYLL 392
E L S P +NF+++V +A S D +G + ++ P+WG + +
Sbjct: 315 PVESRLGSSAASLYPVLNFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGIMVY 371
Query: 393 SLPHDTAHVSVDALK------QPLLTFTSHL--------------LSLTGTPSSGSLPLR 432
++ + S ++ Q + F + L L+G S G +
Sbjct: 372 NVDPKIYNASELPVRVEVDMAQVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWE 431
Query: 433 LSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEA 492
L L RS + L A++TL SLA+L + +I I VA V + + +++A E L
Sbjct: 432 LDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAEALALG 491
Query: 493 E---GLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG----ML 545
++ A +ERAFF+ S++ LYFPD+ K A+Y+PL P+AVP+++ L
Sbjct: 492 HLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFL 551
Query: 546 NELKAWRKRKR 556
K+W+K ++
Sbjct: 552 ETHKSWKKPEK 562
>gb|AAQ89470.1| PIGS [Homo sapiens]
Length = 547
Score = 419 bits (1067), Expect = e-115
Identities = 133/555 (23%), Positives = 236/555 (41%), Gaps = 60/555 (10%)
Query: 53 LSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRIS 112
S++ R + F + ++LGLP+WWKTT +RA+LP ++ + R + P+ +
Sbjct: 2 PSEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGL-NALQLRLMVPVTVV 60
Query: 113 IQANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTI-RLL 171
S+P + + L H + + ++++ A ++ D + AL+ +
Sbjct: 61 FTRESVPLDDQEKLPFTVVH---EREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQ 117
Query: 172 PGDAVTATLDPYEPVLDIT----YPPNAAPSATSAS-------SSLATYVAGQLRSTFAE 220
+A+ + +T ++ S + + + + +
Sbjct: 118 EAEAMLDE-PQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGR 176
Query: 221 EQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSS 280
++ +S PE S KR R LK + Y +TFSL P
Sbjct: 177 RIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKR--RPLKSSLGYEITFSLLNPDPKSHD 234
Query: 281 --WDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV--------LSKDDLSS 330
WDIE A++ Y++P L L NF++D+Q+ YA GV + L L
Sbjct: 235 VYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPH 294
Query: 331 FINAAEWPLSPSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTV 389
IN E L S P +NF+++V +A S D +G + ++ P+WG +
Sbjct: 295 VINPVESRLGSSAASLYPVLNFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGI 351
Query: 390 YLLSLPHDTAHVSVDALK------QPLLTFTSHL--------------LSLTGTPSSGSL 429
+ ++ T + SV ++ + + F + L L+G S G +
Sbjct: 352 MVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLM 411
Query: 430 PLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETL 489
L L RS + L A++TL SLA+L + +I I VA V K + ++++ E L
Sbjct: 412 TWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEEL 471
Query: 490 GEAE---GLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG--- 543
++ A +E AFF+ S++ LYFPD+ K A+Y+PL P+AVP+++
Sbjct: 472 ASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVK 531
Query: 544 -MLNELKAWRKRKRE 557
L K+WRK ++
Sbjct: 532 IFLETRKSWRKPEKT 546
>ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapiens]
sp|Q96S52|PIGS_HUMAN GPI transamidase component PIG-S (Phosphatidylinositol-glycan
biosynthesis, class S protein)
dbj|BAB60853.1| phosphatidyl inositol glycan class S [Homo sapiens]
Length = 555
Score = 417 bits (1063), Expect = e-115
Identities = 132/553 (23%), Positives = 235/553 (41%), Gaps = 60/553 (10%)
Query: 55 DIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQ 114
++ R + F + ++LGLP+WWKTT +RA+LP ++ + R + P+ +
Sbjct: 12 EVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGL-NALQLRLMVPVTVVFT 70
Query: 115 ANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTI-RLLPG 173
S+P + + L H + + ++++ A ++ D + AL+ +
Sbjct: 71 RESVPLDDQEKLPFTVVH---EREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQEA 127
Query: 174 DAVTATLDPYEPVLDIT----YPPNAAPSATSAS-------SSLATYVAGQLRSTFAEEQ 222
+A+ + +T ++ S + + + + +
Sbjct: 128 EAMLDE-PQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRI 186
Query: 223 AIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSS-- 280
++ +S PE S KR R LK + Y +TFSL P
Sbjct: 187 VQVAQAMSLTEDVLAAALADHLPEDKWSAEKR--RPLKSSLGYEITFSLLNPDPKSHDVY 244
Query: 281 WDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV--------LSKDDLSSFI 332
WDIE A++ Y++P L L NF++D+Q+ YA GV + L L I
Sbjct: 245 WDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVI 304
Query: 333 NAAEWPLSPSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYL 391
N E L S P +NF+++V +A S D +G + ++ P+WG + +
Sbjct: 305 NPVESRLGSSAASLYPVLNFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGIMV 361
Query: 392 LSLPHDTAHVSVDALK------QPLLTFTSHL--------------LSLTGTPSSGSLPL 431
++ T + SV ++ + + F + L L+G S G +
Sbjct: 362 YNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTW 421
Query: 432 RLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGE 491
L L RS + L A++TL SLA+L + +I I VA V K + ++++ E L
Sbjct: 422 ELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELAS 481
Query: 492 AE---GLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG----M 544
++ A +E AFF+ S++ LYFPD+ K A+Y+PL P+AVP+++
Sbjct: 482 GHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIF 541
Query: 545 LNELKAWRKRKRE 557
L K+WRK ++
Sbjct: 542 LETRKSWRKPEKT 554
>dbj|BAC11615.1| unnamed protein product [Homo sapiens]
Length = 555
Score = 410 bits (1045), Expect = e-113
Identities = 132/553 (23%), Positives = 234/553 (41%), Gaps = 60/553 (10%)
Query: 55 DIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQ 114
++ R + F + ++LGLP+WWKTT +RA+LP ++ + R + P+ +
Sbjct: 12 EVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGL-NALQLRLMVPVTVVFT 70
Query: 115 ANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTI-RLLPG 173
S+P + + L H + + ++++ A ++ D + AL+ +
Sbjct: 71 RESVPLDDQEKLPFTVVH---EREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQEA 127
Query: 174 DAVTATLDPYEPVLDIT----YPPNAAPSATSAS-------SSLATYVAGQLRSTFAEEQ 222
+A+ + +T ++ S + + + + +
Sbjct: 128 EAMLDE-PQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRI 186
Query: 223 AIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSS-- 280
++ +S PE S KR R LK + Y +TFSL P
Sbjct: 187 VQVAQAMSLTEDVLAAALADHLPEDKWSAEKR--RPLKSSLGYEITFSLLNPDPKSHDVY 244
Query: 281 WDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV--------LSKDDLSSFI 332
WDIE A++ Y++P L L NF++D+Q+ YA GV + L L I
Sbjct: 245 WDIEGAVRRYVQPSLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVI 304
Query: 333 NAAEWPLSPSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYL 391
N E L S P +NF+++V +A S D +G + ++ P+WG + +
Sbjct: 305 NPVESRLGSSAASLYPVLNFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGIMV 361
Query: 392 LSLPHDTAHVSVDALK------QPLLTFTSHL--------------LSLTGTPSSGSLPL 431
++ T + SV ++ + + F + L L+G S G +
Sbjct: 362 YNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTW 421
Query: 432 RLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGE 491
L L RS + L A++TL SLA+L + +I I VA V K + ++++ E L
Sbjct: 422 ELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELAS 481
Query: 492 AE---GLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG----M 544
++ A +E AFF+ S++ LYFPD K A+Y+PL P+AVP+++
Sbjct: 482 GHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDYQKFAIYIPLFLPMAVPILLSLVKIF 541
Query: 545 LNELKAWRKRKRE 557
L K+WRK ++
Sbjct: 542 LETRKSWRKPEKT 554
>ref|XP_203412.1| similar to phosphatidylinositol glycan class S [Mus musculus]
gb|AAH58979.1| Unknown (protein for MGC:67343) [Mus musculus]
Length = 555
Score = 405 bits (1032), Expect = e-111
Identities = 128/542 (23%), Positives = 235/542 (42%), Gaps = 58/542 (10%)
Query: 64 VSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISIQANSLPEQEA 123
+ F + +LLGLP+WWKTT +RA LP ++ + R + P+ + +S+P +
Sbjct: 21 LFFAAVAILLGLPLWWKTTETYRAPLPYSDISGL-NALLLRLMVPVTVVFTRDSVPLDDQ 79
Query: 124 QNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRNDEPDTALTI-RLLPGDAVTATLDP 182
+ L H + + ++++ A ++ + + AL++ + +A+ A +
Sbjct: 80 EKLPFTVVH---EREIPLKYKMKIKCRFQKAYRRALEHEEEALSLGSVHEAEAMLAEPEK 136
Query: 183 YEPVLDITY---PPNAAPSATSAS-------SSLATYVAGQLRSTFAEEQAIISYLLSSN 232
Y ++ S + + + + + ++ +S
Sbjct: 137 QAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREAFNIIGRRIVQVAQAMSLT 196
Query: 233 SIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSS--WDIEAAIQDY 290
PE S KR R LK + Y +TFSL P WDIE A+Q +
Sbjct: 197 EDVLAAALADHLPEDKWSSDKR--RPLKSSLGYEITFSLLNPDPKSHDVHWDIEGAVQRF 254
Query: 291 LKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV--------LSKDDLSSFINAAEWPLSPS 342
++P L LS NF++D+Q+ YA GV + L+ L IN E L S
Sbjct: 255 VQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSLPHVINPVESRLGSS 314
Query: 343 IGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSLPHDTAHV 401
P ++F+++V +A S D +G + ++ P+WG + + ++ +
Sbjct: 315 AASLYPVLHFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGIMVYNVDPKIYNA 371
Query: 402 SVDALK------QPLLTFTSHL--------------LSLTGTPSSGSLPLRLSTLSRIRS 441
S ++ + + F + L L+G S G + L L RS
Sbjct: 372 SELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWELDRLLWARS 431
Query: 442 ADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEAE---GLEHA 498
+ L A++TL SLA+L + +I I VA V + + +++A + L +
Sbjct: 432 VENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKALALGHLSSAFAAS 491
Query: 499 RIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLN----ELKAWRKR 554
+ A +ERAFF+ S++ LYFPD+ K A+Y+PL P+AVP+++ ++ K+W+K
Sbjct: 492 QEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFQETRKSWKKP 551
Query: 555 KR 556
++
Sbjct: 552 EK 553
>ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Saccharomyces
cerevisiae]
sp|Q04080|GP17_YEAST GPI transamidase component GPI17
pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
gb|AAB64866.1| Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
Length = 534
Score = 380 bits (967), Expect = e-104
Identities = 130/552 (23%), Positives = 227/552 (40%), Gaps = 75/552 (13%)
Query: 54 SDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPVFPLRISI 113
S+ R WV F I L LG+P+W+K TT++RA+LP+ + + K + + +
Sbjct: 2 SNANLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYV 61
Query: 114 QANSLPEQEAQNLLR-LTQHALDDLNDFSGHHLR-LQLSSPAAPQKRNDEPDTALTIRLL 171
++++ + + ++ H L+ L+ L + + +T++L
Sbjct: 62 KSDTYRFPDVHDAIQVQVNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKLD 121
Query: 172 PGDAVTATLDPYEPVLDITYPPNAAPSATSASSSLATYVAGQLRSTFAEEQAIISYLLSS 231
++ D + Y A S +A + + TF E ++
Sbjct: 122 EFIGYSSAYD--TKETLVYYDDAAVLSNDLPF-FVAQTL---VEHTFQLEWTHLN----- 170
Query: 232 NSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFTSGPLPSSWDIEAAIQDYL 291
+ E ++ ++ Y P HL+ +L + P +W+IE + DY
Sbjct: 171 --------------KTCEGVSTNNDVAISYDPNIHLSVTLLSGDGNPVAWEIEPTLTDYF 216
Query: 292 KPVLGLLSPIHNFTIDTQV------QLYASPGVQNQVLSKD-----DLSSFINAAEWP-- 338
P LSP+ NFT+D+ + L++ G V D DLS + A +P
Sbjct: 217 SPFRKFLSPLVNFTVDSSIVYHNDLNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPED 276
Query: 339 -------LSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYL 391
+ PS +P + + F+ I S E S+L+PQWG + +
Sbjct: 277 SALNLAIVFPSASSSP--DGLAFINGTRI---------SDEITTLDWNSYLVPQWGVIII 325
Query: 392 LSLP-HDTAHVSVDALKQPLLTFTSHLLSLTGTPSSGSLPL-----RLSTLSRIRSADLL 445
+P + +S D L+ + F + + L G + GS L + + R+ L
Sbjct: 326 NKMPLKPNSVISEDYLEPMMYRFATDIFQLLGL-TEGSQDLLSPYITIDSFKRLTILQNL 384
Query: 446 LRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEA---------EGLE 496
+A+ TL SL +L + +SIP V+D V + + Q + L + L
Sbjct: 385 DKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNALH 444
Query: 497 HARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELKAW-RKRK 555
+ E+AFF MV Q +FP EH +AVYLPLLGP++ + G N +K +K K
Sbjct: 445 LSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSK 504
Query: 556 REAAEKGKEKKK 567
+ E+ K+K
Sbjct: 505 KNGTEREVAKEK 516
>ref|NP_187374.2| expressed protein [Arabidopsis thaliana]
gb|AAL32707.1| Unknown protein [Arabidopsis thaliana]
gb|AAM91117.1| unknown protein [Arabidopsis thaliana]
Length = 599
Score = 351 bits (892), Expect = 3e-95
Identities = 93/348 (26%), Positives = 144/348 (40%), Gaps = 55/348 (15%)
Query: 258 SLKYAPTYHLTFSLFTSGPLP--SSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYAS 315
+ L+FSL S P WD + + L PV L+PI N T+++QV LY +
Sbjct: 251 PVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQV-LYHT 309
Query: 316 P---------GVQNQVLSKDDLSSFINAAEWPLSPSI---GGAPTVNFIVFVGN---QTI 360
P +Q+ + DL F+N+ EW L S G + + F+V++ + +
Sbjct: 310 PKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPL 369
Query: 361 ALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSL--------PHDTAHVSVDALKQPLLT 412
L N S G ++ P WG V + + +S+ L+Q +
Sbjct: 370 LLQLPNVEISKTNG------FISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEI 423
Query: 413 FTSHLLSLTG-----------------TPSSGSLPLRLSTLSRIRSADLLLRASSTLGSL 455
F L G T G L LSR + L S+TLGSL
Sbjct: 424 FLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSL 483
Query: 456 ARLALALKSISIPSSVADGVSKTI--VHLRQACETLGEA-EGLEHARIAEAEAERAFFEK 512
+RL +L + I + + V ++ L Q+ +LG +R A + AE AFF
Sbjct: 484 SRLVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHP 543
Query: 513 SMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELKAWRKRKREAAE 560
S++ YF EH AVY P PV +V+ + E W++ K+E A+
Sbjct: 544 SIMSVSYFSYEHCFAVYSPFFLPVVGHVVLAAVRE---WKRYKQEKAK 588
Score = 41.3 bits (96), Expect = 0.082
Identities = 26/99 (26%), Positives = 37/99 (37%), Gaps = 1/99 (1%)
Query: 63 IVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWADGKACRPV-FPLRISIQANSLPEQ 121
I S L LLG+P WK+ I+R+ LP ++ +D P+ FP
Sbjct: 33 ITSSVLFSFLLGVPFLWKSVEIYRSPLPFHDIDSLSDQLESTPLRFPCNFHAVFVGFRST 92
Query: 122 EAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRND 160
+ NL Q ++ L S S Q R D
Sbjct: 93 DPDNLRSQIQDGINQLTHQSSQCGSCDFSLSVTVQNRED 131
>ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe]
sp|Q10351|PIGS_SCHPO GPI transamidase component PIG-S homolog
pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
(Schizosaccharomyces pombe)
emb|CAA93813.1| SPAC1F12.09 [Schizosaccharomyces pombe]
Length = 554
Score = 343 bits (873), Expect = 5e-93
Identities = 137/542 (25%), Positives = 233/542 (42%), Gaps = 71/542 (13%)
Query: 35 SSATAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEM 94
S + + S + PEK + + ++SF++I+L L +PVWWKTT R++LP ++M
Sbjct: 52 SDSHEVERSNFQLNKPEK----SLKRYALLSFYVIIL-LAIPVWWKTTHYERSSLPFEDM 106
Query: 95 LDWADGKACRPVFPLRISIQANSLPEQEAQNLLRLTQHALD-DLNDFSGHHLRLQLSSPA 153
+ F I + + NL + Q L+ + +S + L+
Sbjct: 107 ENAPSTVQTHLRFSPTFRI-----LDDKGNNLTKEVQKVLEAEPQIYSYNLKVLE----- 156
Query: 154 APQKRNDEPDTALTIRLLPGDAVTATLDPYEPVLDITYPPNAAPSATSASSSLATYVAGQ 213
+ I L + D ++D + + S LA +
Sbjct: 157 -------DDPVDYRIVLRESTDLQWFWDENNFIID---------TPSKGPSELAILIVNC 200
Query: 214 LRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFSLFT 273
L F S + ++ P AE T R+++++P Y + SL
Sbjct: 201 LWEAF-------SPQVMEVWSKFTRFSSTVEPSRAE-----TKRTVQFSPQYRVLLSLLV 248
Query: 274 --SGPLPSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQVLSKD----- 326
P +WDIE AIQ Y P++ L+ + I+TQ+Q + + KD
Sbjct: 249 GEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVEDA---EAYIKDDKFCT 305
Query: 327 ---DLSSFINAAEW--PLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSW 381
DL + +N E SP I PT++F+++V + I DS + + S
Sbjct: 306 KHADLPNLVNNFEKYLSFSPHI-REPTIHFVLYVPSPQIQPLWLENEDSN---IIPTNSM 361
Query: 382 LIPQWGTVYLLSLPHDTAHVSVD-ALKQPLLTFTSHLLSLTG---TPSSGSLPLRLSTLS 437
L+PQWG++ ++ + D LK + LL L G P S L
Sbjct: 362 LLPQWGSITTINFNVTEKKLLHDVDLKDYFRVISRDLLLLLGINDVPVSSLSATLADRLL 421
Query: 438 RIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGE---AEG 494
R R A+ + AS TL +LA+L +++S+++P + V T++ L A + L + E
Sbjct: 422 RQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLNEA 481
Query: 495 LEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLNELK-AWRK 553
L ++ A ++++ A F SMV +YFPDE K +Y PL P+ +PL++ + E+K R+
Sbjct: 482 LSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISFIKEVKDMLRE 541
Query: 554 RK 555
RK
Sbjct: 542 RK 543
>gb|AAF20228.1| unknown protein [Arabidopsis thaliana]
Length = 605
Score = 314 bits (797), Expect = 5e-84
Identities = 84/317 (26%), Positives = 128/317 (39%), Gaps = 52/317 (16%)
Query: 258 SLKYAPTYHLTFSLFTSGPLP--SSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYAS 315
+ L+FSL S P WD + + L PV L+PI N T+++QV LY +
Sbjct: 274 PVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQV-LYHT 332
Query: 316 P---------GVQNQVLSKDDLSSFINAAEWPLSPSI---GGAPTVNFIVFVGN---QTI 360
P +Q+ + DL F+N+ EW L S G + + F+V++ + +
Sbjct: 333 PKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPL 392
Query: 361 ALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSL--------PHDTAHVSVDALKQPLLT 412
L N S G ++ P WG V + + +S+ L+Q +
Sbjct: 393 LLQLPNVEISKTNG------FISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEI 446
Query: 413 FTSHLLSLTG-----------------TPSSGSLPLRLSTLSRIRSADLLLRASSTLGSL 455
F L G T G L LSR + L S+TLGSL
Sbjct: 447 FLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSL 506
Query: 456 ARLALALKSISIPSSVADGVSKTI--VHLRQACETLGEA-EGLEHARIAEAEAERAFFEK 512
+RL +L + I + + V ++ L Q+ +LG +R A + AE AFF
Sbjct: 507 SRLVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHP 566
Query: 513 SMVGQLYFPDEHKVAVY 529
S++ YF EH AVY
Sbjct: 567 SIMSVSYFSYEHCFAVY 583
Score = 37.8 bits (87), Expect = 0.81
Identities = 21/88 (23%), Positives = 32/88 (35%), Gaps = 1/88 (1%)
Query: 74 GLPVWWKTTTIHRANLPLKEMLDWADGKACRPV-FPLRISIQANSLPEQEAQNLLRLTQH 132
G+P WK+ I+R+ LP ++ +D P+ FP + NL Q
Sbjct: 67 GVPFLWKSVEIYRSPLPFHDIDSLSDQLESTPLRFPCNFHAVFVGFRSTDPDNLRSQIQD 126
Query: 133 ALDDLNDFSGHHLRLQLSSPAAPQKRND 160
++ L S S Q R D
Sbjct: 127 GINQLTHQSSQCGSCDFSLSVTVQNRED 154
>gb|AAH01319.2| PIGS protein [Homo sapiens]
Length = 243
Score = 255 bits (646), Expect = 2e-66
Identities = 66/245 (26%), Positives = 115/245 (46%), Gaps = 31/245 (12%)
Query: 341 PSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQSWLIPQWGTVYLLSLPHDTA 399
S P +NF+++V +A S D +G + ++ P+WG + + ++ T
Sbjct: 1 SSAASLYPVLNFLLYVP--ELAHSPLYIQD-KDGAPVATNAFHSPRWGGIMVYNVDSKTY 57
Query: 400 HVSVDALK------QPLLTFTSHL--------------LSLTGTPSSGSLPLRLSTLSRI 439
+ SV ++ + + F + L L+G S G + L L
Sbjct: 58 NASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWA 117
Query: 440 RSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEAE---GLE 496
RS + L A++TL SLA+L + +I I VA V K + ++++ E L
Sbjct: 118 RSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFV 177
Query: 497 HARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG----MLNELKAWR 552
++ A +E AFF+ S++ LYFPD+ K A+Y+PL P+AVP+++ L K+WR
Sbjct: 178 ASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRKSWR 237
Query: 553 KRKRE 557
K ++
Sbjct: 238 KPEKT 242
>dbj|BAB92563.1| P0497A05.6 [Oryza sativa (japonica cultivar-group)]
Length = 557
Score = 244 bits (618), Expect = 5e-63
Identities = 73/310 (23%), Positives = 117/310 (37%), Gaps = 43/310 (13%)
Query: 203 SSSLATYVAGQLRSTFAEEQAIISYLLS-SNSIPTDYRPQSLTPEAAESLAKRTTRSLKY 261
S L V G+ R + + + ++S + Y E S KR +
Sbjct: 210 SDDLKRIVIGKHRHAWVVGKVDEAEVVSIIGKVFVKYFMNGGVEEGEASTVKREFMPVGS 269
Query: 262 APTYHLTFSLFTSGPLP--SSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQL------- 312
L+FSL + P W+ E Q L PV+ L PI N I++QV
Sbjct: 270 DGNIVLSFSLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQVLYHTPKSSY 329
Query: 313 -YASPGVQNQVLSKDDLSSFINAAEWPLSPSI---GGAPTVNFIVFVGNQTIALSSENAH 368
Y+ + VLS D+ F+N+ EW L SI G + + F+V++ + A
Sbjct: 330 SYSDDKLGGNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYIPS---ARECPLYL 386
Query: 369 DSAEGGLTTSQSWLIPQWGTVYLLSLP-------HDTA--HVSVDALKQPLLTFTSHLLS 419
+G L+ + +++ P WG V + + P A +S + L + + F L
Sbjct: 387 QLPDGELSKTNAFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSEELMETIEIFIGQLRQ 446
Query: 420 LTGTPSS-----------------GSLPLRLSTLSRIRSADLLLRASSTLGSLARLALAL 462
L G SS G L L R + LL +TL SL+ L +L
Sbjct: 447 LFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLLYRHHACSNLLSCLTTLESLSSLVQSL 506
Query: 463 KSISIPSSVA 472
+ + +
Sbjct: 507 PRMIVMDEIG 516
Score = 38.2 bits (88), Expect = 0.63
Identities = 24/91 (26%), Positives = 38/91 (41%), Gaps = 12/91 (13%)
Query: 19 ETQMATMTSSPVATPLSSATAAQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVW 78
E A TSSP + +P ++L V+ + L+ L+GLP+
Sbjct: 23 EASGAPSTSSPSDA---DGGGKPSPRTSKPGRKRL--------VLTASVLLSFLIGLPLL 71
Query: 79 WKTTTIHRANLPLKEMLDWADG-KACRPVFP 108
K+T IHR+ LP + + + P FP
Sbjct: 72 LKSTEIHRSPLPSDAIAALSRRLHSNPPSFP 102
>ref|XP_142436.3| similar to phosphatidylinositol glycan class S [Mus musculus]
Length = 225
Score = 195 bits (493), Expect = 2e-48
Identities = 50/193 (25%), Positives = 80/193 (40%), Gaps = 16/193 (8%)
Query: 209 YVAGQLRSTFAEEQAIISYLLSSNSIPTDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLT 268
+ + + ++ +S PE S KR R LK + Y +T
Sbjct: 34 LIHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKR--RPLKSSLGYEIT 91
Query: 269 FSLFTSGPLPSS--WDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV---- 322
FSL P WDIE A+Q +++P L LS NF++D+Q+ YA GV +
Sbjct: 92 FSLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPAS 151
Query: 323 ----LSKDDLSSFINAAEWPLSPSIGGA-PTVNFIVFVGNQTIALSSENAHDSAEGGLTT 377
L+ L IN E L S P ++F+++V +A S D +G
Sbjct: 152 SSYSLAMHSLPHVINPVESRLGSSAASLYPVLHFLLYVP--ELAHSPLYIQD-KDGAPVA 208
Query: 378 SQSWLIPQWGTVY 390
+ ++ P+WG +
Sbjct: 209 TNAFHSPRWGGIM 221
>dbj|BAB70938.1| unnamed protein product [Homo sapiens]
Length = 184
Score = 188 bits (475), Expect = 3e-46
Identities = 56/180 (31%), Positives = 88/180 (48%), Gaps = 24/180 (13%)
Query: 400 HVSVDALKQPLLTFTSHL--------------LSLTGTPSSGSLPLRLSTLSRIRSADLL 445
V VD ++ + F + L L+G S G + L L RS + L
Sbjct: 6 RVEVDMVR-VMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENL 64
Query: 446 LRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETLGEAEGLEHARIAEAE- 504
A++TL SLA+L + +I I VA V K + ++++ E L L A +A E
Sbjct: 65 ATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGH-LASAFVASQEV 123
Query: 505 ---AERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMG----MLNELKAWRKRKRE 557
+E AFF+ S++ LYFPD+ K +Y+PL P+AVP+++ L K+WRK ++
Sbjct: 124 VTSSELAFFDPSLLHLLYFPDDQKFPIYIPLFLPMAVPILLSLVKLFLETRKSWRKPEKT 183
>emb|CAE45912.1| hypothetical protein [Homo sapiens]
Length = 162
Score = 182 bits (459), Expect = 2e-44
Identities = 48/147 (32%), Positives = 77/147 (51%), Gaps = 7/147 (4%)
Query: 418 LSLTGTPSSGSLPLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSK 477
L+G S G + L L RS + L A++TL SLA+L + +I I VA V K
Sbjct: 15 CLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYK 74
Query: 478 TIVHLRQACETLGEAE---GLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLG 534
+ ++++ E L ++ A +E FF+ S++ LYFPD+ K A+Y+PL
Sbjct: 75 AVAAVQKSAEELASGHLASAFVASQEAVTSSELVFFDPSLLHLLYFPDDQKFAIYIPLFL 134
Query: 535 PVAVPLVMG----MLNELKAWRKRKRE 557
P+AVP+++ L K+WRK ++
Sbjct: 135 PMAVPILLSLVKIFLETRKSWRKPEKT 161
>ref|NP_501852.1| phosphatidylinositol glycan class S like (4K912) [Caenorhabditis
elegans]
pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
emb|CAA93093.1| Hypothetical protein T14G10.7 [Caenorhabditis elegans]
Length = 544
Score = 125 bits (312), Expect = 4e-27
Identities = 33/114 (28%), Positives = 61/114 (52%), Gaps = 7/114 (6%)
Query: 430 PLRLSTLSRIR-SADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACET 488
++ +R+R S D +RA+S + +L +LA + +I I VAD ++ + L +
Sbjct: 418 QWEVAR-ARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVADRATRAVEIL---NDV 473
Query: 489 LGEAEGLEHARIAEAE--AERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPL 540
+ L+ R+ EA A+ A + S++ LYFP + + AV +PL P+ +P+
Sbjct: 474 VRPGRMLDFERLLEARRLADSANSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPI 527
Score = 40.1 bits (93), Expect = 0.16
Identities = 12/37 (32%), Positives = 24/37 (64%)
Query: 54 SDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLP 90
S+ ++ +SF ++V +G+P+W TT+ +R+N P
Sbjct: 40 SERPYKLAASLSFVILVFFIGMPMWHFTTSTYRSNFP 76
CPU time: 197.08 user secs. 6.00 sys. secs 203.08 total secs.
Database: nr
Posted date: Nov 22, 2003 1:30 PM
Number of letters in database: 504,497,942
Number of sequences in database: 1,543,518
Lambda K H
0.306 0.160 0.441
Gapped
Lambda K H
0.270 0.0561 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 523387523
Number of Sequences: 1543518
Number of extensions: 21970585
Number of successful extensions: 113040
Number of sequences better than 10.0: 87
Number of HSP's better than 10.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 65
Number of HSP's that attempted gapping in prelim test: 112706
Number of HSP's gapped (non-prelim): 240
length of query: 568
length of database: 504,497,942
effective HSP length: 63
effective length of query: 505
effective length of database: 407,256,308
effective search space: 205664435540
effective search space used: 205664435540
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (20.7 bits)
S2: 78 (34.5 bits)