Sequences with E-value BETTER than threshold (0.002)
ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapie... 888 0.0
ref|XP_203412.1| similar to phosphatidylinositol glycan class S ... 887 0.0
dbj|BAC11615.1| unnamed protein product [Homo sapiens] 880 0.0
ref|XP_220633.1| similar to phosphatidylinositol glycan class S ... 717 0.0
gb|EAA07616.1| agCP1277 [Anopheles gambiae str. PEST] 639 0.0
ref|NP_733060.1| CG31120-PB [Drosophila melanogaster] >gi|169020... 487 e-136
ref|NP_187374.2| unknown protein; protein id: At3g07180.1, suppo... 487 e-136
gb|AAF20228.1|AC012395_15 unknown protein [Arabidopsis thaliana] 448 e-124
ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe... 411 e-113
ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Sacc... 358 3e-97
ref|XP_142436.2| similar to phosphatidylinositol glycan class S ... 345 3e-93
dbj|BAB70938.1| unnamed protein product [Homo sapiens] 260 5e-68
ref|XP_220635.1| similar to phosphatidylinositol glycan class S ... 161 5e-38
ref|NP_501852.1| Putative endoplasmic reticulum membrane protein... 146 1e-33
gb|AAF28987.1|AF161427_1 HSPC309 [Homo sapiens] 95 6e-18
Alignments
>ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapiens]
sp|Q96S52|PIGS_HUMAN GPI transamidase component PIG-S (Phosphatidylinositol-glycan
biosynthesis, class S protein)
dbj|BAB60853.1| phosphatidyl inositol glycan class S [Homo sapiens]
Length = 555
Score = 888 bits (2270), Expect = 0.0
Identities = 510/555 (91%), Positives = 531/555 (94%)
Query: 1 MAAAGAAATDLEVVRGKRSALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGLNALLLR 60
MAAAGAAAT LEV RGKR+ALFFAAVAI+LGLPLWWKTTETYRA LPYS ISGLNAL LR
Sbjct: 1 MAAAGAAATHLEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLR 60
Query: 61 LMVPVTVVFTRDSVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEEALS 120
LMVPVTVVFTR+SVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRAL+HEEEALS
Sbjct: 61 LMVPVTVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALS 120
Query: 121 LGSVHEAEAMLAEPEKQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREAFN 180
GSV EAEAML EP++QAEGSLTVYVISEHSSLLPQDMMSYIGP+RTAVVRG++HREAFN
Sbjct: 121 SGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFN 180
Query: 181 IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLNPDPKS 240
IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWS++KRRPLKSSLGYEITFSLLNPDPKS
Sbjct: 181 IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKS 240
Query: 241 HDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSL 300
HDV+WDIEGAV+R+VQPFLN L AGNFSVDSQILYYAMLGVNPRFD ASSSY L MHSL
Sbjct: 241 HDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSL 300
Query: 301 PHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM 360
PHVINPVESRLGSSAASLYPVL+FLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM
Sbjct: 301 PHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM 360
Query: 361 VYNVDPKIYNASELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMT 420
VYNVD K YNAS LPVRVEVDMVRVMEVFLAQLRLLFGIAQPQ+PPKCLLSGP SEGLMT
Sbjct: 361 VYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMT 420
Query: 421 WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKALA 480
WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVY+AVAAVQK+A+ LA
Sbjct: 421 WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELA 480
Query: 481 LGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKI 540
GHL+SAF ASQEAVTSSE AFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKI
Sbjct: 481 SGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKI 540
Query: 541 FQETRKSWKKPEKID 555
F ETRKSW+KPEK D
Sbjct: 541 FLETRKSWRKPEKTD 555
>ref|XP_203412.1| similar to phosphatidylinositol glycan class S [Homo sapiens] [Mus
musculus]
Length = 555
Score = 887 bits (2268), Expect = 0.0
Identities = 555/555 (100%), Positives = 555/555 (100%)
Query: 1 MAAAGAAATDLEVVRGKRSALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGLNALLLR 60
MAAAGAAATDLEVVRGKRSALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGLNALLLR
Sbjct: 1 MAAAGAAATDLEVVRGKRSALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGLNALLLR 60
Query: 61 LMVPVTVVFTRDSVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEEALS 120
LMVPVTVVFTRDSVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEEALS
Sbjct: 61 LMVPVTVVFTRDSVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEEALS 120
Query: 121 LGSVHEAEAMLAEPEKQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREAFN 180
LGSVHEAEAMLAEPEKQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREAFN
Sbjct: 121 LGSVHEAEAMLAEPEKQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREAFN 180
Query: 181 IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLNPDPKS 240
IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLNPDPKS
Sbjct: 181 IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLNPDPKS 240
Query: 241 HDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSL 300
HDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSL
Sbjct: 241 HDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSL 300
Query: 301 PHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM 360
PHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM
Sbjct: 301 PHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM 360
Query: 361 VYNVDPKIYNASELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMT 420
VYNVDPKIYNASELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMT
Sbjct: 361 VYNVDPKIYNASELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMT 420
Query: 421 WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKALA 480
WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKALA
Sbjct: 421 WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKALA 480
Query: 481 LGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKI 540
LGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKI
Sbjct: 481 LGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKI 540
Query: 541 FQETRKSWKKPEKID 555
FQETRKSWKKPEKID
Sbjct: 541 FQETRKSWKKPEKID 555
>dbj|BAC11615.1| unnamed protein product [Homo sapiens]
Length = 555
Score = 880 bits (2251), Expect = 0.0
Identities = 508/555 (91%), Positives = 529/555 (94%)
Query: 1 MAAAGAAATDLEVVRGKRSALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGLNALLLR 60
MAAAGAAAT LEV RGKR+ALFFAAVAI+LGLPLWWKTTETYRA LPYS ISGLNAL LR
Sbjct: 1 MAAAGAAATHLEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLR 60
Query: 61 LMVPVTVVFTRDSVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEEALS 120
LMVPVTVVFTR+SVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRAL+HEEEALS
Sbjct: 61 LMVPVTVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALS 120
Query: 121 LGSVHEAEAMLAEPEKQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREAFN 180
GSV EAEAML EP++QAEGSLTVYVISEHSSLLPQDMMSYIGP+RTAVVRG++HREAFN
Sbjct: 121 SGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFN 180
Query: 181 IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLNPDPKS 240
IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWS++KRRPLKSSLGYEITFSLLNPDPKS
Sbjct: 181 IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKS 240
Query: 241 HDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSL 300
HDV+WDIEGAV+R+VQP LN L AGNFSVDSQILYYAMLGVNPRFD ASSSY L MHSL
Sbjct: 241 HDVYWDIEGAVRRYVQPSLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSL 300
Query: 301 PHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM 360
PHVINPVESRLGSSAASLYPVL+FLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM
Sbjct: 301 PHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM 360
Query: 361 VYNVDPKIYNASELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMT 420
VYNVD K YNAS LPVRVEVDMVRVMEVFLAQLRLLFGIAQPQ+PPKCLLSGP SEGLMT
Sbjct: 361 VYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMT 420
Query: 421 WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKALA 480
WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVY+AVAAVQK+A+ LA
Sbjct: 421 WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELA 480
Query: 481 LGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKI 540
GHL+SAF ASQEAVTSSE AFFDPSLLHLLYFPD QKFAIYIPLFLPMAVPILLSLVKI
Sbjct: 481 SGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDYQKFAIYIPLFLPMAVPILLSLVKI 540
Query: 541 FQETRKSWKKPEKID 555
F ETRKSW+KPEK D
Sbjct: 541 FLETRKSWRKPEKTD 555
>ref|XP_220633.1| similar to phosphatidylinositol glycan class S [Homo sapiens]
[Rattus norvegicus]
Length = 496
Score = 717 bits (1833), Expect = 0.0
Identities = 430/469 (91%), Positives = 439/469 (92%), Gaps = 10/469 (2%)
Query: 97 KMKIKCRFQKAYRRALEHEEEALSLGSVHEAEAMLAEPEKQAEGSLTVYVISEHSSLLPQ 156
KMKIKCRFQKAYRRALEHEEEALSLGSVHEAEA+LAEPEKQAEGSLTVYVISEHSSLLPQ
Sbjct: 28 KMKIKCRFQKAYRRALEHEEEALSLGSVHEAEALLAEPEKQAEGSLTVYVISEHSSLLPQ 87
Query: 157 DMMSYIGPERTAVVRGLIHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSD 216
DMMSYIGPERTA+VRG+IHREAFNIIGRRI+ VAQAMSLTEDVLAAALADHLPEDKWSSD
Sbjct: 88 DMMSYIGPERTAIVRGMIHREAFNIIGRRIIHVAQAMSLTEDVLAAALADHLPEDKWSSD 147
Query: 217 KRRPLKSSLGYEITFSLLNPDPKSHDVHWDIEGA----VQRFVQP------FLNRLSVAG 266
KRRPLKSSLGYEITFSLLNPDPKSHDVHWDI G F++P FL LS
Sbjct: 148 KRRPLKSSLGYEITFSLLNPDPKSHDVHWDIGGGCPTLCAAFLEPPQRCRQFLCGLSAIC 207
Query: 267 NFSVDSQILYYAMLGVNPRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYPVLHFLL 326
QILYYAMLGVNPRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYPVL+FLL
Sbjct: 208 RAVSCEQILYYAMLGVNPRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYPVLNFLL 267
Query: 327 YVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDPKIYNASELPVRVEVDMVRVM 386
YVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDPKIYNASELPVRVEVDM +VM
Sbjct: 268 YVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDPKIYNASELPVRVEVDMAQVM 327
Query: 387 EVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWELDRLLWARSVENLATATTTLTSLA 446
EVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWELDRLLWARSVENLATATTTLTSLA
Sbjct: 328 EVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWELDRLLWARSVENLATATTTLTSLA 387
Query: 447 QLLGKISNIVIKDDVASEVYRAVAAVQKAAKALALGHLSSAFAASQEAVTSSERAFFDPS 506
QLLGKISNIVIKDDVASEVYRAVAAVQKAA+ALALGHLSSAFAASQEAVTSSERAFFDPS
Sbjct: 388 QLLGKISNIVIKDDVASEVYRAVAAVQKAAEALALGHLSSAFAASQEAVTSSERAFFDPS 447
Query: 507 LLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFQETRKSWKKPEKID 555
LLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIF ET KSWKKPEKID
Sbjct: 448 LLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETHKSWKKPEKID 496
>gb|EAA07616.1| agCP1277 [Anopheles gambiae str. PEST]
Length = 590
Score = 639 bits (1632), Expect = 0.0
Identities = 186/553 (33%), Positives = 282/553 (50%), Gaps = 53/553 (9%)
Query: 19 SALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGLNALLLRLMVPVTVVFTRDSVPLDD 78
+ + F + I++G+P+WWKTTE YR LPYS+I LN P+ F R +
Sbjct: 60 ATIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNE------DPIKATF-RLGLYCQS 112
Query: 79 QEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEEAL---SLGSVHEAEAMLAEPE 135
E+ REI L ++++ K F++ + L+ + +L +L ++ + AE +
Sbjct: 113 AER--------REI-LSFELRKK--FEQNFVFELDLQLLSLDADTLSTIKTPARLEAELQ 161
Query: 136 KQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREAFNIIGRRIVQVAQAMSL 195
K +L ++ E L D++ + +RTA I A ++ + QV + L
Sbjct: 162 KHYPSTLGHFLFIEWPK-LDDDIL--VTSDRTA----FISESASSL---KAYQVLSSWLL 211
Query: 196 TEDVLAAALADHLPEDKWSSDKRR----PLKSSLGYEITFSLLNPDPKSHDVHWDIEGAV 251
E L A L + + R PL++ YE+ ++LNP P+ VHW+ A
Sbjct: 212 QEYKLKAILGSRDAQQTLMGRQLRLNSAPLQA--QYEVLITVLNPRPELQKVHWNARAAA 269
Query: 252 QRFVQPFLNRLSVAGNFSVDSQILYYAML---GVNPRFDPASSS----YSLAMHSLPHVI 304
+ +++PFL+ LS NF++ +Q +Y L G P+ P S Y+LA SLPH+I
Sbjct: 270 ENYIEPFLSELSGITNFTIKTQWIYQVGLEGAGAQPKQVPDDSKLGRHYALAEDSLPHII 329
Query: 305 NPVESRLGSSAASLYPVLHFLLYVPELAHSPLYIQDKDG---APVATN--AFHSPRWGGI 359
+E +LG+ P +H ++YVP A +PL I KDG AP+A+N AF S +WGGI
Sbjct: 330 TSLEKKLGTQITD-NPCIHLVVYVPPCAQAPLRIYRKDGQRAAPLASNVEAFTSAKWGGI 388
Query: 360 MVYNVDPKI---YNASELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSE 416
+ N Y +E V + VM V L QLR +F + L +
Sbjct: 389 VFANPAEATCVRYMETEQFSDVYIHAQDVMPVLLYQLRKIFDLENNTPLLDATLVPYSTI 448
Query: 417 GLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAA 476
WE+D + ++ + +ATTTL SL QLLG I IVI D+V + + A + A
Sbjct: 449 EPRAWEVDTFMRTNTIYLVHSATTTLQSLIQLLGGIEYIVINDEVGAAIQTAHRKIVAAK 508
Query: 477 KALALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLS 536
+ L G L A ++EA TS+ERAFFDPS+L LLYFPD+QK+AIYIPLFLP+ +P++ S
Sbjct: 509 QQLRAGRLQQAALLAREAYTSAERAFFDPSMLALLYFPDEQKYAIYIPLFLPIMIPVVFS 568
Query: 537 LVKIFQETRKSWK 549
I + RK
Sbjct: 569 FNTILKYFRKRKA 581
>ref|NP_733060.1| CG31120-PB [Drosophila melanogaster]
gb|AAL27645.1| SD10476p [Drosophila melanogaster]
gb|AAF56366.2| CG31120-PB [Drosophila melanogaster]
Length = 536
Score = 487 bits (1242), Expect = e-136
Identities = 147/548 (26%), Positives = 254/548 (45%), Gaps = 56/548 (10%)
Query: 19 SALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGLNALLLRLMVPVTVVFTRDSVPLDD 78
+ L F V I +G+P+WW+TT YR LP ++I L+ + ++ V V ++
Sbjct: 25 ATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSKIPIKTAVQV-------AIYTQQ 77
Query: 79 QEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEEALSLGS--VHEAEAMLAEPEK 136
+ + +++ F ++E ++ + + + H A+ +
Sbjct: 78 TSRGQLLIA-----------ELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAALEKLLLE 126
Query: 137 QAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREAFNIIGRRIVQVAQAMSLT 196
S+ ++ E L + ++ ER+A++R + +I Q+ A L
Sbjct: 127 NHVQSVGDFMFIEWPKLQEELLL---TTERSALMR-------SDTPSNKIAQLLHAKILQ 176
Query: 197 EDVLAAALADHLPEDKWSSDKRRPLKS---SLGYEITFSLLNPDPKSHDVHWDIEGAVQR 253
+ L S+D+R KS Y++ S+LNP P+ W+I AV+
Sbjct: 177 TYRINQIL---------STDERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKT 227
Query: 254 FVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSS----YSLAMHSLPHVINPVES 309
+++P+L ++S N++V SQ Y + + + S Y+L +LPH++ +
Sbjct: 228 YIEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQ 287
Query: 310 RLGSSAASLYPVLHFLLYVPELAHSPLYIQDKDGAPVATN---AFHSPRWGGIMVYNVDP 366
L +S P ++ ++Y+P +PL+I + + N AF SP WGG ++ N
Sbjct: 288 NLSASTTD-KPAINLVVYIPPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPE 346
Query: 367 KIYNAS---ELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWEL 423
+ A+ + V V M+V L QL L I+ + + WE
Sbjct: 347 HVCLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEY 406
Query: 424 DRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKALALGH 483
+ L ++ +++TA++TL SL +LL +IS IVI D+V + + + A + A AL
Sbjct: 407 EAYLRRSAIRHISTASSTLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHR 466
Query: 484 LSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSL---VKI 540
L+ A ++ A +SER FFD SLL LYFPD+QK+AIYIPLFLP+ VP+L S +
Sbjct: 467 LADASVLAKRAFVASERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSFNMLRGV 526
Query: 541 FQETRKSW 548
Q RK
Sbjct: 527 LQARRKEK 534
>ref|NP_187374.2| unknown protein; protein id: At3g07180.1, supported by cDNA:
gi_17065105 [Arabidopsis thaliana]
gb|AAL32707.1| Unknown protein [Arabidopsis thaliana]
gb|AAM91117.1| unknown protein [Arabidopsis thaliana]
Length = 599
Score = 487 bits (1241), Expect = e-136
Identities = 107/383 (27%), Positives = 186/383 (47%), Gaps = 9/383 (2%)
Query: 176 REAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLN 235
R A+ + + ++ ++ + E+ + P+ S ++FSLLN
Sbjct: 207 RHAWIVGNGLEERYGDIVARVSEIFVQVFMNGGREEDSIQGEFMPVGSDGKIVLSFSLLN 266
Query: 236 PDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSSYSL 295
+P+ WD + + + P L+ N +V+SQ+LY+ +D SY
Sbjct: 267 SNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSSFSSWDKKLQSYIF 326
Query: 296 AMHSLPHVINPVESRLGSSAAS--LYPVLHFLLYVPELAHSPLYIQDKDGAPVATNAFHS 353
LP +N E L +SA + +L F++Y+P PL +Q + TN F S
Sbjct: 327 RTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLLQLPNVEISKTNGFIS 386
Query: 354 PRWGGIMVYNVDPKIYNASELPVRVEV---DMVRVMEVFLAQLRLLFGIAQPQVPPKCLL 410
P WGG++V+N SE P R + D+ +++E+FL Q R LFG L
Sbjct: 387 PMWGGVIVWN-PGNCDKDSESPSRNTISLQDLEQIVEIFLGQFRQLFGFKSEAKYTTGLG 445
Query: 411 SGP---KSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYR 467
+ G WELD L + NL + +TTL SL++L+ + ++IKD++ +V
Sbjct: 446 TYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSRLVRSLPRMIIKDEIGEQVKY 505
Query: 468 AVAAVQKAAKALALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFL 527
++ A + A +LG SS+ ++S+EA + +E AFF PS++ + YF + FA+Y P FL
Sbjct: 506 SLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHPSIMSVSYFSYEHCFAVYSPFFL 565
Query: 528 PMAVPILLSLVKIFQETRKSWKK 550
P+ ++L+ V+ ++ ++ K
Sbjct: 566 PVVGHVVLAAVREWKRYKQEKAK 588
Score = 38.9 bits (90), Expect = 0.33
Identities = 15/31 (48%), Positives = 20/31 (64%)
Query: 24 AAVAILLGLPLWWKTTETYRAPLPYSDISGL 54
+ LLG+P WK+ E YR+PLP+ DI L
Sbjct: 37 VLFSFLLGVPFLWKSVEIYRSPLPFHDIDSL 67
>gb|AAF20228.1|AC012395_15 unknown protein [Arabidopsis thaliana]
Length = 605
Score = 448 bits (1141), Expect = e-124
Identities = 100/355 (28%), Positives = 170/355 (47%), Gaps = 9/355 (2%)
Query: 176 REAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLN 235
R A+ + + ++ ++ + E+ + P+ S ++FSLLN
Sbjct: 230 RHAWIVGNGLEERYGDIVARVSEIFVQVFMNGGREEDSIQGEFMPVGSDGKIVLSFSLLN 289
Query: 236 PDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASSSYSL 295
+P+ WD + + + P L+ N +V+SQ+LY+ +D SY
Sbjct: 290 SNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSSFSSWDKKLQSYIF 349
Query: 296 AMHSLPHVINPVESRLGSSAAS--LYPVLHFLLYVPELAHSPLYIQDKDGAPVATNAFHS 353
LP +N E L +SA + +L F++Y+P PL +Q + TN F S
Sbjct: 350 RTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLLQLPNVEISKTNGFIS 409
Query: 354 PRWGGIMVYNVDPKIYNASELPVRVEV---DMVRVMEVFLAQLRLLFGIAQPQVPPKCLL 410
P WGG++V+N SE P R + D+ +++E+FL Q R LFG L
Sbjct: 410 PMWGGVIVWN-PGNCDKDSESPSRNTISLQDLEQIVEIFLGQFRQLFGFKSEAKYTTGLG 468
Query: 411 SGP---KSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYR 467
+ G WELD L + NL + +TTL SL++L+ + ++IKD++ +V
Sbjct: 469 TYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSRLVRSLPRMIIKDEIGEQVKY 528
Query: 468 AVAAVQKAAKALALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIY 522
++ A + A +LG SS+ ++S+EA + +E AFF PS++ + YF + FA+Y
Sbjct: 529 SLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHPSIMSVSYFSYEHCFAVY 583
Score = 37.3 bits (86), Expect = 1.1
Identities = 14/29 (48%), Positives = 20/29 (68%)
Query: 26 VAILLGLPLWWKTTETYRAPLPYSDISGL 54
V+ + G+P WK+ E YR+PLP+ DI L
Sbjct: 62 VSEVSGVPFLWKSVEIYRSPLPFHDIDSL 90
>ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe]
sp|Q10351|PIGS_SCHPO GPI transamidase component PIG-S homolog
pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
(Schizosaccharomyces pombe)
emb|CAA93813.1| hypothetical protein [Schizosaccharomyces pombe]
Length = 554
Score = 411 bits (1047), Expect = e-113
Identities = 128/506 (25%), Positives = 222/506 (43%), Gaps = 64/506 (12%)
Query: 61 LMVPV---TVVFTRDSVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEE 117
L +PV T + R S+P +D E P TV + RF +R L+ +
Sbjct: 85 LAIPVWWKTTHYERSSLPFEDMENAPSTV------------QTHLRFSPTFRI-LDDKGN 131
Query: 118 ALSLGSVHEAEA-MLAEPEKQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHR 176
L+ E + + AEP+ + S + +L D + Y R +
Sbjct: 132 NLT----KEVQKVLEAEPQ----------IYSYNLKVLEDDPVDYRIVLRESTDLQWFWD 177
Query: 177 EAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWS-------------SDKRRPLKS 223
E II ++ L + L A + WS ++ +R ++
Sbjct: 178 ENNFIIDTPSKGPSELAILIVNCLWEAF-SPQVMEVWSKFTRFSSTVEPSRAETKRTVQF 236
Query: 224 SLGYEITFSLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVN 283
S Y + SLL + ++WDIE A+Q++ P + +L+ ++++QI Y+ +
Sbjct: 237 SPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVE---D 293
Query: 284 PRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLYIQDKDG 343
+ LP+++N E L S P +HF+LYVP PL+++++D
Sbjct: 294 AEAYIKDDKFCTKHADLPNLVNNFEKYLSFSPHIREPTIHFVLYVPSPQIQPLWLENEDS 353
Query: 344 APVATNAFHSPRWGGIMVYNVDPKIYNASELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQ 403
+ TN+ P+WG I N +N +E + +VD+ V L LL GI
Sbjct: 354 NIIPTNSMLLPQWGSITTIN-----FNVTEKKLLHDVDLKDYFRVISRDLLLLLGINDVP 408
Query: 404 VPPKCLLSGPKSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVAS 463
V L DRLL R E+ A+ TL +LA+L+ + ++ + ++
Sbjct: 409 VS-----------SLSATLADRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQM 457
Query: 464 EVYRAVAAVQKAAKALALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYI 523
V + ++ A KAL+ +L+ A + S A + S+ A F PS++ +YFPD+ K+ IY
Sbjct: 458 YVKDTLLSLDMAYKALSQNNLNEALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYA 517
Query: 524 PLFLPMAVPILLSLVKIFQETRKSWK 549
PLF P+ +P+L+S +K ++ + K
Sbjct: 518 PLFAPILIPLLISFIKEVKDMLRERK 543
Score = 41.6 bits (97), Expect = 0.057
Identities = 18/34 (52%), Positives = 22/34 (63%)
Query: 17 KRSALFFAAVAILLGLPLWWKTTETYRAPLPYSD 50
KR AL V ILL +P+WWKTT R+ LP+ D
Sbjct: 72 KRYALLSFYVIILLAIPVWWKTTHYERSSLPFED 105
>ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Saccharomyces
cerevisiae]
sp|Q04080|GP17_YEAST GPI transamidase component GPI17
pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
gb|AAB64866.1| Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
Length = 534
Score = 358 bits (910), Expect = 3e-97
Identities = 83/356 (23%), Positives = 149/356 (41%), Gaps = 40/356 (11%)
Query: 211 DKWSSDKRRPLKSSLGYEITFSLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSV 270
+ S++ + ++ +LL+ D + V W+IE + + PF LS NF+V
Sbjct: 174 EGVSTNNDVAISYDPNIHLSVTLLSGD--GNPVAWEIEPTLTDYFSPFRKFLSPLVNFTV 231
Query: 271 DSQILYYAMLGVNPRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYP---VLHFLLY 327
DS I+Y+ L ++ S S+ L H I+ E S+ + YP L+ +
Sbjct: 232 DSSIVYHNDLNLHSL---NGSCTSVTWFDLSHTIDLSE----LSSMAYYPEDSALNLAIV 284
Query: 328 VPELAHSPLYIQDKDGAPVAT-------NAFHSPRWGGIMVYNVDPKIYNASELPVRVEV 380
P + SP + +G ++ N++ P+W G+++ N P N+ +E
Sbjct: 285 FPSASSSPDGLAFINGTRISDEITTLDWNSYLVPQW-GVIIINKMPLKPNSVISEDYLEP 343
Query: 381 DMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWELDRLLWARSVENLATATT 440
M R L L G +Q + P + K ++ +NL AT
Sbjct: 344 MMYRFATDIFQLLGLTEG-SQDLLSPYITIDSFKRLTIL-------------QNLDKATE 389
Query: 441 TLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKAL----ALGHL--SSAFAASQEA 494
TL SL +L + + I +V+ V A+ + L G + ++A S E
Sbjct: 390 TLWSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNEL 449
Query: 495 VTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFQETRKSWKK 550
V E+AFF+ ++ +FP + A+Y+PL P++ + + +E + KK
Sbjct: 450 VKLCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSKK 505
Score = 43.6 bits (102), Expect = 0.014
Identities = 22/54 (40%), Positives = 30/54 (54%), Gaps = 3/54 (5%)
Query: 17 KRSALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGL--NALL-LRLMVPVTV 67
K F A+ + LG+PLW+K T YRA LP + I L N + L++PV V
Sbjct: 8 KWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYV 61
>ref|XP_142436.2| similar to phosphatidylinositol glycan class S [Homo sapiens] [Mus
musculus]
Length = 207
Score = 345 bits (876), Expect = 3e-93
Identities = 203/203 (100%), Positives = 203/203 (100%)
Query: 158 MMSYIGPERTAVVRGLIHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDK 217
MMSYIGPERTAVVRGLIHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDK
Sbjct: 1 MMSYIGPERTAVVRGLIHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDK 60
Query: 218 RRPLKSSLGYEITFSLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYY 277
RRPLKSSLGYEITFSLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYY
Sbjct: 61 RRPLKSSLGYEITFSLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYY 120
Query: 278 AMLGVNPRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLY 337
AMLGVNPRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLY
Sbjct: 121 AMLGVNPRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLY 180
Query: 338 IQDKDGAPVATNAFHSPRWGGIM 360
IQDKDGAPVATNAFHSPRWGGIM
Sbjct: 181 IQDKDGAPVATNAFHSPRWGGIM 203
>dbj|BAB70938.1| unnamed protein product [Homo sapiens]
Length = 184
Score = 260 bits (660), Expect = 5e-68
Identities = 165/181 (91%), Positives = 172/181 (94%)
Query: 375 PVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWELDRLLWARSVEN 434
PVRVEVDMVRVMEVFLAQLRLLFGIAQPQ+PPKCLLSGP SEGLMTWELDRLLWARSVEN
Sbjct: 4 PVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVEN 63
Query: 435 LATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKALALGHLSSAFAASQEA 494
LATATTTLTSLAQLLGKISNIVIKDDVASEVY+AVAAVQK+A+ LA GHL+SAF ASQE
Sbjct: 64 LATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEV 123
Query: 495 VTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFQETRKSWKKPEKI 554
VTSSE AFFDPSLLHLLYFPDDQKF IYIPLFLPMAVPILLSLVK+F ETRKSW+KPEK
Sbjct: 124 VTSSELAFFDPSLLHLLYFPDDQKFPIYIPLFLPMAVPILLSLVKLFLETRKSWRKPEKT 183
Query: 555 D 555
D
Sbjct: 184 D 184
>ref|XP_220635.1| similar to phosphatidylinositol glycan class S [Homo sapiens]
[Rattus norvegicus]
Length = 108
Score = 161 bits (404), Expect = 5e-38
Identities = 81/84 (96%), Positives = 83/84 (98%)
Query: 12 EVVRGKRSALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGLNALLLRLMVPVTVVFTR 71
EVVRGKR+ALFFAAVAILLGLPLWWKTTETYRAPLPYS+ISGLNAL LRLMVPVTVVFTR
Sbjct: 21 EVVRGKRAALFFAAVAILLGLPLWWKTTETYRAPLPYSEISGLNALQLRLMVPVTVVFTR 80
Query: 72 DSVPLDDQEKLPFTVVHEREIPLK 95
DSVPLDDQEKLPFTVVHEREIPLK
Sbjct: 81 DSVPLDDQEKLPFTVVHEREIPLK 104
>ref|NP_501852.1| Putative endoplasmic reticulum membrane protein, with at least 2
transmembrane domains, a coiled coil-4 domain, of
bilaterial origin [Caenorhabditis elegans]
pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
emb|CAA93093.1| Hypothetical protein T14G10.7 [Caenorhabditis elegans]
Length = 544
Score = 146 bits (367), Expect = 1e-33
Identities = 51/127 (40%), Positives = 67/127 (52%), Gaps = 8/127 (6%)
Query: 420 TWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKAL 479
WE+ R SV+N A + + +L QL KISNIVI DDVA RAV + +
Sbjct: 418 QWEVARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVADRATRAVEILNDVVRP- 476
Query: 480 ALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVK 539
G + F EA ++ A D SLL +LYFP DQ+ A+ IPL AVPI+L + K
Sbjct: 477 --GRMLD-FERLLEARRLADSANSDHSLLAMLYFPMDQRTAVLIPL----AVPIVLPICK 529
Query: 540 IFQETRK 546
+ E K
Sbjct: 530 LVYEIAK 536
Score = 35.0 bits (80), Expect = 4.7
Identities = 15/51 (29%), Positives = 25/51 (48%), Gaps = 6/51 (11%)
Query: 19 SALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGLNALLLRLMVPVTVVF 69
++L F + +G+P+W TT TYR+ P + VP+ V+F
Sbjct: 48 ASLSFVILVFFIGMPMWHFTTSTYRSNFPTF------PSNQSITVPIRVIF 92
>gb|AAF28987.1|AF161427_1 HSPC309 [Homo sapiens]
Length = 162
Score = 94.6 bits (233), Expect = 6e-18
Identities = 30/66 (45%), Positives = 33/66 (49%)
Query: 352 HSPRWGGIMVYNVDPKIYNASELPVRVEVDMVRVMEVFLAQLRLLFGIAQPQVPPKCLLS 411
P GIMVYNVD K YNAS LPVRVEVDMVRV G+ P
Sbjct: 97 IVPAGVGIMVYNVDSKTYNASVLPVRVEVDMVRVWRCSWHSCGCSLGLLSPSCLQNACFQ 156
Query: 412 GPKSEG 417
G + +G
Sbjct: 157 GLRVKG 162
CPU time: 155.85 user secs. 4.90 sys. secs 160.75 total secs.
Database: nr
Posted date: Jan 18, 2003 1:23 PM
Number of letters in database: 421,297,171
Number of sequences in database: 1,315,992
Lambda K H
0.311 0.166 0.465
Gapped
Lambda K H
0.270 0.0581 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 476170437
Number of Sequences: 1315992
Number of extensions: 21400382
Number of successful extensions: 50379
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 50309
Number of HSP's gapped (non-prelim): 41
length of query: 555
length of database: 421,297,171
effective HSP length: 60
effective length of query: 495
effective length of database: 342,337,651
effective search space: 169457137245
effective search space used: 169457137245
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.0 bits)
S2: 77 (34.1 bits)