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GPI Anchor Biosynthesis Report: Q10351 (PIG-S family, Schizosaccharomyces pombe)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (554 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 15 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

sp|Q10351|YDA9_SCHPO HYPOTHETICAL 63.9 KD PROTEIN C1F12.09 IN CH... 828 0.0
gb|AAL27645.1| (AY061834) SD10476p [Drosophila melanogaster] 578 e-164
ref|NP_010722.1| (NC_001136) Glycosyl Phosphatidyl Inositol 17; ... 565 e-160
ref|NP_149975.1| (NM_033198) phosphatidylinositol glycan, class ... 554 e-156
gb|AAL32707.1| (AY062629) Unknown protein [Arabidopsis thaliana] 484 e-135
ref|NP_187374.1| (NM_111598) unknown protein [Arabidopsis thalia... 441 e-122
gb|AAF56365.1| (AE003749) CG13644 gene product [Drosophila melan... 401 e-110
ref|NP_501852.1| (NM_069451) T14G10.7.p [Caenorhabditis elegans]... 378 e-103
dbj|BAB70938.1| (AK055512) unnamed protein product [Homo sapiens] 199 1e-49
ref|XP_091596.1| (XM_091596) similar to phosphatidylinositol gly... 195 1e-48
gb|AAF56366.1| (AE003749) CG6031 gene product [Drosophila melano... 156 9e-37
Sequences with E-value WORSE than threshold

ref|NP_126656.1| (NC_000868) SIMILAR TO SEVERAL DNA HELICASES [P... 36 1.9
Alignments
>sp|Q10351|YDA9_SCHPO HYPOTHETICAL 63.9 KD PROTEIN C1F12.09 IN CHROMOSOME I
 pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 pir||T38067 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA93813.1| (Z69944) hypothetical protein [Schizosaccharomyces pombe]
          Length = 554

 Score =  828 bits (2116), Expect = 0.0
 Identities = 554/554 (100%), Positives = 554/554 (100%)

Query: 1   MSPCKWLTMFLKGCWGKIANMNHLDSCFPLRYIIGRKKASKLLCTMDSGLKSDSHEVERS 60
           MSPCKWLTMFLKGCWGKIANMNHLDSCFPLRYIIGRKKASKLLCTMDSGLKSDSHEVERS
Sbjct: 1   MSPCKWLTMFLKGCWGKIANMNHLDSCFPLRYIIGRKKASKLLCTMDSGLKSDSHEVERS 60

Query: 61  NFQLNKPEKSLKRYALLSFYVIILLAIPVWWKTTHYERSSLPFEDMENAPSTVQTHLRFS 120
           NFQLNKPEKSLKRYALLSFYVIILLAIPVWWKTTHYERSSLPFEDMENAPSTVQTHLRFS
Sbjct: 61  NFQLNKPEKSLKRYALLSFYVIILLAIPVWWKTTHYERSSLPFEDMENAPSTVQTHLRFS 120

Query: 121 PTFRILDDKGNNLTKEVQKVLEAEPQIYSYNLKVLEDDPVDYRIVLRESTDLQWFWDENN 180
           PTFRILDDKGNNLTKEVQKVLEAEPQIYSYNLKVLEDDPVDYRIVLRESTDLQWFWDENN
Sbjct: 121 PTFRILDDKGNNLTKEVQKVLEAEPQIYSYNLKVLEDDPVDYRIVLRESTDLQWFWDENN 180

Query: 181 FIIDTPSKGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSSTVEPSRAETKRTVQFSPQY 240
           FIIDTPSKGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSSTVEPSRAETKRTVQFSPQY
Sbjct: 181 FIIDTPSKGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSSTVEPSRAETKRTVQFSPQY 240

Query: 241 RVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVEDAEAYIKD 300
           RVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVEDAEAYIKD
Sbjct: 241 RVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVEDAEAYIKD 300

Query: 301 DKFCTKHADLPNLVNNFEKYLSFSPHIREPTIHFVLYVPSPQIQPLWLENEDSNIIPTNS 360
           DKFCTKHADLPNLVNNFEKYLSFSPHIREPTIHFVLYVPSPQIQPLWLENEDSNIIPTNS
Sbjct: 301 DKFCTKHADLPNLVNNFEKYLSFSPHIREPTIHFVLYVPSPQIQPLWLENEDSNIIPTNS 360

Query: 361 MLLPQWGSITTINFNVTEKKLLHDVDLKDYFRVISRDLLLLLGINDVPVSSLSATLADRL 420
           MLLPQWGSITTINFNVTEKKLLHDVDLKDYFRVISRDLLLLLGINDVPVSSLSATLADRL
Sbjct: 361 MLLPQWGSITTINFNVTEKKLLHDVDLKDYFRVISRDLLLLLGINDVPVSSLSATLADRL 420

Query: 421 LRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLNE 480
           LRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLNE
Sbjct: 421 LRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLNE 480

Query: 481 ALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISFIKEVKDMLR 540
           ALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISFIKEVKDMLR
Sbjct: 481 ALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISFIKEVKDMLR 540

Query: 541 ERKLHRVANVPKPN 554
           ERKLHRVANVPKPN
Sbjct: 541 ERKLHRVANVPKPN 554
>gb|AAL27645.1| (AY061834) SD10476p [Drosophila melanogaster]
          Length = 536

 Score =  578 bits (1476), Expect = e-164
 Identities = 119/517 (23%), Positives = 221/517 (42%), Gaps = 61/517 (11%)

Query: 65  NKPEKSLKRYALLSFYVIIL-LAIPVWWKTTHYERSSLPFEDMENA-----PSTVQTHLR 118
           +K E   +  A L+F V+I+ + +P+WW+TT   R +LP  ++ +       + VQ  + 
Sbjct: 15  DKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSKIPIKTAVQVAIY 74

Query: 119 FSPTFR---ILDDKGNNLT---------KEVQKVLEAEPQIYSYNL-KVLEDDPVDYRIV 165
              T R   ++ +  N  +         K++      +       L K+L ++ V     
Sbjct: 75  TQQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAALEKLLLENHVQ---S 131

Query: 166 LRESTDLQWFWDENNFIIDT--PSKGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSSTV 223
           + +   ++W   +   ++ T   +   S+     +  L  A   Q   +     +  ST 
Sbjct: 132 VGDFMFIEWPKLQEELLLTTERSALMRSDTPSNKIAQLLHAKILQTYRI----NQILSTD 187

Query: 224 EPSRAETKRTVQFSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNI 283
           E   A+++      P Y V++S+L  +       W+I  A++ Y  P + +++ ++   +
Sbjct: 188 ERMGAKSEAP---QPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTV 244

Query: 284 ETQIQYFV--EDAEAYIKDDKFCTKH-----ADLPNLVNNFEKYLSFSPHIREPTIHFVL 336
            +Q +Y V  E     ++D     +H       LP+L+ +  + LS S    +P I+ V+
Sbjct: 245 RSQWKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSAST-TDKPAINLVV 303

Query: 337 YVPSPQIQPLWLENEDSNIIPTN---SMLLPQWGSITTIN--------FNVTEKKLLHDV 385
           Y+P   I PL + N     +  N   + + P WG     N            ++ + + V
Sbjct: 304 YIPPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHV 363

Query: 386 DLKDYFRVISRDLLLLL---------GINDVPVSSLSATL--ADRLLRQRIAESCIEASD 434
              D  +V+   L  LL         G+  V +  L       +  LR+        AS 
Sbjct: 364 STTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASS 423

Query: 435 TLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLNEALSYSNNAFSKSQE 494
           TLQ+L KL+  +  + +  E+   + ++   +  A  AL ++ L +A   +  AF  S+ 
Sbjct: 424 TLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVASER 483

Query: 495 ALFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISF 531
             F  S++  +YFPDE KY IY PLF PI++P+L SF
Sbjct: 484 GFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSF 520
>ref|NP_010722.1| (NC_001136) Glycosyl Phosphatidyl Inositol 17; Gpi17p
           [Saccharomyces cerevisiae]
 pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
 gb|AAB64866.1| (U33007) Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
          Length = 534

 Score =  565 bits (1441), Expect = e-160
 Identities = 112/513 (21%), Positives = 214/513 (40%), Gaps = 52/513 (10%)

Query: 70  SLKRYALLSFYVII-LLAIPVWWKTTHYERSSLPFEDMENAPST--VQTHLRF-----SP 121
           +L+++    F  I   L +P+W+K T   R+SLP   +E+  +      HL       S 
Sbjct: 5   NLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYVKSD 64

Query: 122 TFRILDDKGNNLTKEVQKVLEAEPQIYSYNLKVL---------EDDPVDYRIV---LRES 169
           T+R   D  + +  +V  +L ++ Q   ++L+VL         E +   + +V   L E 
Sbjct: 65  TYR-FPDVHDAIQVQVNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKLDEF 123

Query: 170 TDLQWFWDENNFII--DTPSKGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSSTVEPSR 227
                 +D    ++  D  +   ++L   +   L E           ++T  + T E   
Sbjct: 124 IGYSSAYDTKETLVYYDDAAVLSNDLPFFVAQTLVE------HTFQLEWTHLNKTCEGVS 177

Query: 228 AETKRTVQFSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQI 287
                 + + P   + ++LL G+GN  P+ W+IE  +  YF+P  + L+ L    +++ I
Sbjct: 178 TNNDVAISYDPNIHLSVTLLSGDGN--PVAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSI 235

Query: 288 QYFVEDAEAYIKDDKFCTKHADLPNLVNNFEKYLSFSPHIREPTIHFVLYVPSPQIQPLW 347
            Y  +     +          DL + + +  +  S + +  +  ++  +  PS    P  
Sbjct: 236 VYHNDLNLHSLNGSCTSVTWFDLSHTI-DLSELSSMAYYPEDSALNLAIVFPSASSSPDG 294

Query: 348 LE-------NEDSNIIPTNSMLLPQWGSITTINFNVTEKKLLHDVDLKDYFRVISRDLLL 400
           L        +++   +  NS L+PQWG I      +    ++ +  L+      + D+  
Sbjct: 295 LAFINGTRISDEITTLDWNSYLVPQWGVIIINKMPLKPNSVISEDYLEPMMYRFATDIFQ 354

Query: 401 LLGINDVPVSSLSATL-ADRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYV 459
           LLG+ +     LS  +  D   R  I ++  +A++TL +L KL    Q M++P+E+    
Sbjct: 355 LLGLTEGSQDLLSPYITIDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVSD-- 412

Query: 460 KDTLLSLDMAYKALSQNN---------LNEALSYSNNAFSKSQEALFHPSMVTTIYFPDE 510
            + + +LD+  + +   N          N AL  SN      ++A F+  MV   +FP E
Sbjct: 413 -NVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQE 471

Query: 511 SKYGIYAPLFAPILIPLLISFIKEVKDMLRERK 543
               +Y PL  PI   +   F   +K+  ++ K
Sbjct: 472 HMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSK 504
>ref|NP_149975.1| (NM_033198) phosphatidylinositol glycan, class S [Homo sapiens]
 dbj|BAB60853.1| (AB057723) phosphatidyl inositol glycan class S [Homo sapiens]
          Length = 555

 Score =  554 bits (1414), Expect = e-156
 Identities = 131/529 (24%), Positives = 222/529 (41%), Gaps = 73/529 (13%)

Query: 72  KRYALLSFYVIILLAIPVWWKTTHYERSSL----------------------------PF 103
           KR AL    V I+L +P+WWKTT   R+SL                            P 
Sbjct: 17  KRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLRLMVPVTVVFTRESVPL 76

Query: 104 EDMENAPSTVQTHLRFSP---TFRILDDKGNNLTKEVQKVLEAEPQIYSYNLK----VLE 156
           +D E  P TV  H R  P     +I         + +      E  + S +++    +L+
Sbjct: 77  DDQEKLPFTV-VHEREIPLKYKMKIKCRFQKAYRRALDH---EEEALSSGSVQEAEAMLD 132

Query: 157 DDPVDYRIVLRESTDLQWFWDENNFIIDTPSKGPSELAILIVNCLWEAFSPQVMEVWSKF 216
           +        L      +        ++     GP   A++      EAF+     +    
Sbjct: 133 EPQEQAEGSLTVYVISEHSSLLPQDMM--SYIGPKRTAVVRGIMHREAFNIIGRRIVQVA 190

Query: 217 TRFSSTVEPS-------------RAETKRTVQFSPQYRVLLSLLVGEGNHEPINWDIENA 263
              S T +                AE +R ++ S  Y +  SLL  +     + WDIE A
Sbjct: 191 QAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGA 250

Query: 264 IQKYFNPLIEQLASLAKLNIETQIQYFVEDAEAYIKDDKFCTKHAD---LPNLVNNFEKY 320
           +++Y  P +  L +    ++++QI Y+         D    + + D   LP+++N  E  
Sbjct: 251 VRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVINPVESR 310

Query: 321 LSFSPHIREPTIHFVLYVPSPQIQPLWLENEDSNIIPTNSMLLPQWGSITTIN-----FN 375
           L  S     P ++F+LYVP     PL+++++D   + TN+   P+WG I   N     +N
Sbjct: 311 LGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDSKTYN 370

Query: 376 VTEKKLLHDVDLKDYFRVISRDLLLLLGIND---VPVSSLSATLA--------DRLLRQR 424
            +   +  +VD+     V    L LL GI      P   LS   +        DRLL  R
Sbjct: 371 ASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWAR 430

Query: 425 IAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLNEALSY 484
             E+   A+ TL +LA+L+  + ++ +  ++   V   + ++  + + L+  +L  A   
Sbjct: 431 SVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVA 490

Query: 485 SNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISFIK 533
           S  A + S+ A F PS++  +YFPD+ K+ IY PLF P+ +P+L+S +K
Sbjct: 491 SQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVK 539
>gb|AAL32707.1| (AY062629) Unknown protein [Arabidopsis thaliana]
          Length = 599

 Score =  484 bits (1233), Expect = e-135
 Identities = 91/386 (23%), Positives = 159/386 (40%), Gaps = 29/386 (7%)

Query: 186 PSKGPSELAILIVNCLWEAFSPQVMEVWSKFTR--FSSTVEPSRAETK-RTVQFSPQYRV 242
              G    A ++ N L E +   V  V   F +   +   E    + +   V    +  +
Sbjct: 201 AVVGKRRHAWIVGNGLEERYGDIVARVSEIFVQVFMNGGREEDSIQGEFMPVGSDGKIVL 260

Query: 243 LLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVEDAEAYIKDDK 302
             SLL         +WD +   +    P+ + LA +A + +E+Q+ Y    +     D K
Sbjct: 261 SFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSSFSSWDKK 320

Query: 303 ---FCTKHADLPNLVNNFEKYLSFSPHI--REPTIHFVLYVPSPQIQPLWLENEDSNIIP 357
              +  + +DLP  VN+ E +L  S     R   + FV+Y+PS +  PL L+  +  I  
Sbjct: 321 LQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLLQLPNVEISK 380

Query: 358 TNSMLLPQWGSITTIN-FNVTEKK------LLHDVDLKDYFRVISRDLLLLLGINDVPV- 409
           TN  + P WG +   N  N  +         +   DL+    +       L G       
Sbjct: 381 TNGFISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEIFLGQFRQLFGFKSEAKY 440

Query: 410 -SSLSATLA------------DRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQ 456
            + L                 D L R+    +    S TL +L++LV S+  M +  EI 
Sbjct: 441 TTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSRLVRSLPRMIIKDEIG 500

Query: 457 MYVKDTLLSLDMAYKALSQNNLNEALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIY 516
             VK +L +  +A    S    + + S S  A S ++ A FHPS+++  YF  E  + +Y
Sbjct: 501 EQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHPSIMSVSYFSYEHCFAVY 560

Query: 517 APLFAPILIPLLISFIKEVKDMLRER 542
           +P F P++  ++++ ++E K   +E+
Sbjct: 561 SPFFLPVVGHVVLAAVREWKRYKQEK 586
 Score = 37.3 bits (86), Expect = 0.59
 Identities = 12/38 (31%), Positives = 19/38 (49%)

Query: 72  KRYALLSFYVIILLAIPVWWKTTHYERSSLPFEDMENA 109
           + +   S     LL +P  WK+    RS LPF D+++ 
Sbjct: 30  RLFITSSVLFSFLLGVPFLWKSVEIYRSPLPFHDIDSL 67
>ref|NP_187374.1| (NM_111598) unknown protein [Arabidopsis thaliana]
 gb|AAF20228.1|AC012395_15 (AC012395) unknown protein [Arabidopsis thaliana]
          Length = 605

 Score =  441 bits (1124), Expect = e-122
 Identities = 85/361 (23%), Positives = 143/361 (39%), Gaps = 29/361 (8%)

Query: 186 PSKGPSELAILIVNCLWEAFSPQVMEVWSKFTR--FSSTVEPSRAETK-RTVQFSPQYRV 242
              G    A ++ N L E +   V  V   F +   +   E    + +   V    +  +
Sbjct: 224 AVVGKRRHAWIVGNGLEERYGDIVARVSEIFVQVFMNGGREEDSIQGEFMPVGSDGKIVL 283

Query: 243 LLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVEDAEAYIKDDK 302
             SLL         +WD +   +    P+ + LA +A + +E+Q+ Y    +     D K
Sbjct: 284 SFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSSFSSWDKK 343

Query: 303 ---FCTKHADLPNLVNNFEKYLSFSPHI--REPTIHFVLYVPSPQIQPLWLENEDSNIIP 357
              +  + +DLP  VN+ E +L  S     R   + FV+Y+PS +  PL L+  +  I  
Sbjct: 344 LQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLLQLPNVEISK 403

Query: 358 TNSMLLPQWGSITTIN-FNVTEKK------LLHDVDLKDYFRVISRDLLLLLGINDVPV- 409
           TN  + P WG +   N  N  +         +   DL+    +       L G       
Sbjct: 404 TNGFISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEIFLGQFRQLFGFKSEAKY 463

Query: 410 -SSLSATLA------------DRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQ 456
            + L                 D L R+    +    S TL +L++LV S+  M +  EI 
Sbjct: 464 TTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSRLVRSLPRMIIKDEIG 523

Query: 457 MYVKDTLLSLDMAYKALSQNNLNEALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIY 516
             VK +L +  +A    S    + + S S  A S ++ A FHPS+++  YF  E  + +Y
Sbjct: 524 EQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHPSIMSVSYFSYEHCFAVY 583

Query: 517 A 517
           +
Sbjct: 584 S 584
 Score = 34.6 bits (79), Expect = 4.6
 Identities = 14/43 (32%), Positives = 21/43 (48%)

Query: 67  PEKSLKRYALLSFYVIILLAIPVWWKTTHYERSSLPFEDMENA 109
            E S+    L  F V  +  +P  WK+    RS LPF D+++ 
Sbjct: 48  SEFSISLPLLYPFPVSEVSGVPFLWKSVEIYRSPLPFHDIDSL 90
>gb|AAF56365.1| (AE003749) CG13644 gene product [Drosophila melanogaster]
          Length = 347

 Score =  401 bits (1021), Expect = e-110
 Identities = 85/335 (25%), Positives = 150/335 (44%), Gaps = 33/335 (9%)

Query: 226 SRAETKRTVQFSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIET 285
             A+++      P Y V++S+L  +       W+I  A++ Y  P + +++ ++   + +
Sbjct: 1   MGAKSEAP---QPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRS 57

Query: 286 QIQYFV--EDAEAYIKDDKFCTKH-----ADLPNLVNNFEKYLSFSPHIREPTIHFVLYV 338
           Q +Y V  E     ++D     +H       LP+L+ +  + LS S    +P I+ V+Y+
Sbjct: 58  QWKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSAST-TDKPAINLVVYI 116

Query: 339 PSPQIQPLWLENEDSNIIPTN---SMLLPQWGSITTIN--------FNVTEKKLLHDVDL 387
           P   I PL + N     +  N   + + P WG     N            ++ + + V  
Sbjct: 117 PPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVST 176

Query: 388 KDYFRVISRDLLLLL---------GINDVPVSSLSATL--ADRLLRQRIAESCIEASDTL 436
            D  +V+   L  LL         G+  V +  L       +  LR+        AS TL
Sbjct: 177 TDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTL 236

Query: 437 QNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLNEALSYSNNAFSKSQEAL 496
           Q+L KL+  +  + +  E+   + ++   +  A  AL ++ L +A   +  AF  S+   
Sbjct: 237 QSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGF 296

Query: 497 FHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISF 531
           F  S++  +YFPDE KY IY PLF PI++P+L SF
Sbjct: 297 FDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSF 331
>ref|NP_501852.1| (NM_069451) T14G10.7.p [Caenorhabditis elegans]
 pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
 emb|CAA93093.1| (Z68880) predicted using Genefinder~cDNA EST EMBL:M89453 comes from
           this gene~cDNA EST yk111h3.3 comes from this gene~cDNA
           EST yk111h3.5 comes from this gene~cDNA EST yk457b10.3
           comes from this gene~cDNA EST yk457b10.5 comes from this
           gene~cDNA EST yk3>
          Length = 544

 Score =  378 bits (962), Expect = e-103
 Identities = 65/438 (14%), Positives = 148/438 (32%), Gaps = 59/438 (13%)

Query: 111 STVQTHLR-----FSPTFRILDDKGNNLTKEVQKVLEAEPQIYSYNLKVLEDDPVDYRIV 165
           +T    LR            +++        V  V E E   YS             ++ 
Sbjct: 128 TTQFIGLRSFEHLEQDHSIQIEENKEFFDVFVALVPEKEWTHYSAT---------RIQLG 178

Query: 166 LRESTDLQWFWDENN-----FIIDTPSKGPSELAILI--VNCLWEAFSPQVMEVWSKFTR 218
           + +   +QW             +       SE  I +  +N +      Q M+ W     
Sbjct: 179 IGKWNFIQWPTKSEEKQKASTRVTDLV---SETLIDVPHLNSIVRRDLRQKMQPWQI--- 232

Query: 219 FSSTVEPSRAETKRTVQFSP---QYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQL 275
                 P   + +     +P    Y + +  L       P +   +    +      E++
Sbjct: 233 ---AALPLSHQKRLVWDSAPLATNYHIQVIHLHENSEITPESLKEQEKTARALRKFAEKV 289

Query: 276 ASLAKLNIETQIQY-FVEDAEAYIKD--DKFCTKHADLPNLVNNFEKYLSFSPHIREPTI 332
             +  + +  +  + F       IKD  +++   H+ +  LV   +  L  S       +
Sbjct: 290 KEITNVEVSFEHLWDFEPTRGFLIKDVQERWALTHSAMEELVKKVDSQLQTSLQ-HGAVL 348

Query: 333 HFVLYVPSPQIQPLWLENEDSNIIPTNSMLLPQWGSITTINFNVTEKKLLHDVDLKDYFR 392
            FV+      ++P+ + ++      ++ + +  WG++   N   TE + +  + ++    
Sbjct: 349 RFVVL---ETVEPVIVLDDTGE--DSHGVAVASWGAVLPRN-EATESRAIAAIRIQMGM- 401

Query: 393 VISRDLLLLLGINDVPVSSLSATLADRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVP 452
               D  L  G +  PV+     +A   LR    ++ + A+  ++ L +L   + ++ + 
Sbjct: 402 ----DAELSHGWHRPPVAICQWEVARARLRA-SVDNAMRAASAVRALDQLAQKISNIVIN 456

Query: 453 KEIQMYVKDTLL---SLDMAYKALSQNNLNEALSYSNNAFSKSQEALFHPSMVTTIYFPD 509
            ++       +     +    + L    L         A   +  A    S++  +YFP 
Sbjct: 457 DDVADRATRAVEILNDVVRPGRMLDFERLL-------EARRLADSANSDHSLLAMLYFPM 509

Query: 510 ESKYGIYAPLFAPILIPL 527
           + +  +  PL  PI++P+
Sbjct: 510 DQRTAVLIPLAVPIVLPI 527
 Score =  128 bits (320), Expect = 2e-28
 Identities = 26/121 (21%), Positives = 42/121 (34%), Gaps = 22/121 (18%)

Query: 56  EVERSNFQLNKPEKSLKRYALLSFYV-IILLAIPVWWKTTHYERSSLPFEDMENAPSTVQ 114
           E+ +S       E+  K  A LSF + +  + +P+W  TT   RS+ P       PS   
Sbjct: 29  EITQSFINYVHSERPYKLAASLSFVILVFFIGMPMWHFTTSTYRSNFP-----TFPSNQS 83

Query: 115 THLRFSPTFRILDD--------KGNNLTKEVQKVLEAEPQIYSYNLKV--------LEDD 158
             +     F I  +          N L   + K     P  +++  +         LE D
Sbjct: 84  ITVPIRVIFVITSEDLRNDVKTLMNELEVNMTKKEVVAPLDFTWTTQFIGLRSFEHLEQD 143

Query: 159 P 159
            
Sbjct: 144 H 144
>dbj|BAB70938.1| (AK055512) unnamed protein product [Homo sapiens]
          Length = 184

 Score =  199 bits (502), Expect = 1e-49
 Identities = 48/167 (28%), Positives = 82/167 (48%), Gaps = 11/167 (6%)

Query: 378 EKKLLHDVDLKDYFRVISRDLLLLLGIND---VPVSSLSATLA--------DRLLRQRIA 426
              +  +VD+     V    L LL GI      P   LS   +        DRLL  R  
Sbjct: 2   PFPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSV 61

Query: 427 ESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLNEALSYSN 486
           E+   A+ TL +LA+L+  + ++ +  ++   V   + ++  + + L+  +L  A   S 
Sbjct: 62  ENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQ 121

Query: 487 NAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISFIK 533
              + S+ A F PS++  +YFPD+ K+ IY PLF P+ +P+L+S +K
Sbjct: 122 EVVTSSELAFFDPSLLHLLYFPDDQKFPIYIPLFLPMAVPILLSLVK 168
>ref|XP_091596.1| (XM_091596) similar to phosphatidylinositol glycan, class S (H.
           sapiens) [Homo sapiens]
          Length = 204

 Score =  195 bits (493), Expect = 1e-48
 Identities = 30/183 (16%), Positives = 64/183 (34%), Gaps = 22/183 (12%)

Query: 122 TFRILDDKGNNLTKEVQKVLEAEPQIYSYNLK----VLEDDPVDYRIVLRESTDLQWFWD 177
             +I         + +      E  + S +++    +L++        L      +    
Sbjct: 22  KMKIKCRFQKAYRRALDH---EEEALSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSL 78

Query: 178 ENNFIIDTPSKGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSSTVEPS----------- 226
               ++     GP   A++      EAF+     +       S T +             
Sbjct: 79  LPQDMM--SYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPED 136

Query: 227 --RAETKRTVQFSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIE 284
              AE +R ++ S  Y +  SLL  +     + WDIE A+++Y  P +  L +    +++
Sbjct: 137 KWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVD 196

Query: 285 TQI 287
           +Q+
Sbjct: 197 SQV 199
>gb|AAF56366.1| (AE003749) CG6031 gene product [Drosophila melanogaster]
          Length = 164

 Score =  156 bits (392), Expect = 9e-37
 Identities = 28/150 (18%), Positives = 62/150 (40%), Gaps = 24/150 (16%)

Query: 65  NKPEKSLKRYALLSFYVIIL-LAIPVWWKTTHYERSSLPFEDMENA-----PSTVQTHLR 118
           +K E   +  A L+F V+I+ + +P+WW+TT   R +LP  ++ +       + VQ  + 
Sbjct: 15  DKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSKIPIKTAVQVAIY 74

Query: 119 FSPTFR---ILDDKGNNLT---------KEVQKVLEAEPQIYSYNL-KVLEDDPVDYRIV 165
              T R   ++ +  N  +         K++      +       L K+L ++ V     
Sbjct: 75  TQQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAALEKLLLENHVQ---S 131

Query: 166 LRESTDLQWFWDENNFIIDT--PSKGPSEL 193
           + +   ++W   +   ++ T   +   S+ 
Sbjct: 132 VGDFMFIEWPKLQEELLLTTERSALMRSDT 161
>ref|NP_126656.1| (NC_000868) SIMILAR TO SEVERAL DNA HELICASES [Pyrococcus abyssi]
 pir||B75073 ATP-dependent RNA helicase homolog PAB1722 - Pyrococcus abyssi
           (strain Orsay)
 emb|CAB49887.1| (AJ248286) SIMILAR TO SEVERAL DNA HELICASES [Pyrococcus abyssi]
          Length = 872

 Score = 35.8 bits (82), Expect = 1.9
 Identities = 21/129 (16%), Positives = 46/129 (35%), Gaps = 17/129 (13%)

Query: 190 PSELAILIVNCLWEAFSPQVMEVWSKFTRFSSTVE-------PSRAETKRTVQFSPQYRV 242
           P+ +AI   N +    +         F      +E       P   + +R  +    Y +
Sbjct: 374 PNRVAI---NGIVLRRNGLDYYYKEHFEELVEGIEKGLVEGIPVNLDNERIAKKHLHYLL 430

Query: 243 L---LSLLVGEGNHEPIN---WDIENAIQKYFNPLIEQLA-SLAKLNIETQIQYFVEDAE 295
               +  +     H  I       E  +++Y NPL  ++   + K  I + I+   +++ 
Sbjct: 431 SELGVVDVNEIPEHWRIALRPLKEEGIVEEYLNPLTNKVEIRIKKPAIYSSIRSTSDESF 490

Query: 296 AYIKDDKFC 304
             +KD+ + 
Sbjct: 491 FLVKDEGWI 499
CPU time:   111.77 user secs.	    2.64 sys. secs	  114.41 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.314    0.169    0.459 

Gapped
Lambda     K      H
   0.270   0.0591    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 303954436
Number of Sequences: 887402
Number of extensions: 13423063
Number of successful extensions: 33745
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 33656
Number of HSP's gapped (non-prelim): 30
length of query: 554
length of database: 277,845,442
effective HSP length: 58
effective length of query: 496
effective length of database: 226,376,126
effective search space: 112282558496
effective search space used: 112282558496
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.1 bits)
S2: 76 (33.7 bits)