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GPI Anchor Biosynthesis Report: NP_149975.1 (PIG-S family, Homo sapiens)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (555 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 15 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_149975.1| (NM_033198) phosphatidylinositol glycan, class ... 884 0.0
gb|AAL32707.1| (AY062629) Unknown protein [Arabidopsis thaliana] 548 e-155
ref|NP_187374.1| (NM_111598) unknown protein [Arabidopsis thalia... 522 e-147
gb|AAL27645.1| (AY061834) SD10476p [Drosophila melanogaster] 453 e-126
gb|AAF56365.1| (AE003749) CG13644 gene product [Drosophila melan... 445 e-124
sp|Q10351|YDA9_SCHPO HYPOTHETICAL 63.9 KD PROTEIN C1F12.09 IN CH... 417 e-115
ref|NP_010722.1| (NC_001136) Glycosyl Phosphatidyl Inositol 17; ... 372 e-102
ref|XP_091596.1| (XM_091596) similar to phosphatidylinositol gly... 317 4e-85
dbj|BAB70938.1| (AK055512) unnamed protein product [Homo sapiens] 262 1e-68
ref|NP_501852.1| (NM_069451) T14G10.7.p [Caenorhabditis elegans]... 199 1e-49
gb|AAF28987.1|AF161427_1 (AF161427) HSPC309 [Homo sapiens] 80 8e-14
Sequences with E-value WORSE than threshold

gb|AAF56366.1| (AE003749) CG6031 gene product [Drosophila melano... 42 0.033
Alignments
>ref|NP_149975.1| (NM_033198) phosphatidylinositol glycan, class S [Homo sapiens]
 dbj|BAB60853.1| (AB057723) phosphatidyl inositol glycan class S [Homo sapiens]
          Length = 555

 Score =  884 bits (2260), Expect = 0.0
 Identities = 555/555 (100%), Positives = 555/555 (100%)

Query: 1   MAAAGAAATHLEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLR 60
           MAAAGAAATHLEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLR
Sbjct: 1   MAAAGAAATHLEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLR 60

Query: 61  LMVPVTVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALS 120
           LMVPVTVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALS
Sbjct: 61  LMVPVTVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALS 120

Query: 121 SGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFN 180
           SGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFN
Sbjct: 121 SGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFN 180

Query: 181 IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKS 240
           IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKS
Sbjct: 181 IIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKS 240

Query: 241 HDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSL 300
           HDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSL
Sbjct: 241 HDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSL 300

Query: 301 PHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM 360
           PHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM
Sbjct: 301 PHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM 360

Query: 361 VYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMT 420
           VYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMT
Sbjct: 361 VYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMT 420

Query: 421 WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELA 480
           WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELA
Sbjct: 421 WELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELA 480

Query: 481 SGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKI 540
           SGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKI
Sbjct: 481 SGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKI 540

Query: 541 FLETRKSWRKPEKTD 555
           FLETRKSWRKPEKTD
Sbjct: 541 FLETRKSWRKPEKTD 555
>gb|AAL32707.1| (AY062629) Unknown protein [Arabidopsis thaliana]
          Length = 599

 Score =  548 bits (1399), Expect = e-155
 Identities = 118/429 (27%), Positives = 207/429 (47%), Gaps = 31/429 (7%)

Query: 136 EQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRI----VQVAQ 191
           E +    +V V+++ ++    ++ + +G +R A + G    E +  I  R+    VQV  
Sbjct: 177 ENSGKMYSVVVVNKENANGGDEVKAVVGKRRHAWIVGNGLEERYGDIVARVSEIFVQVFM 236

Query: 192 AMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAV 251
                ED +                   P+ S     ++FSLLN +P+     WD +   
Sbjct: 237 NGGREEDSIQGEFM--------------PVGSDGKIVLSFSLLNSNPRDWVYDWDFQRID 282

Query: 252 RRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVINPVESRL 311
              + P   AL    N +V+SQ+LY+        +D    SY      LP  +N  E  L
Sbjct: 283 EALLAPVTKALAPIANITVESQVLYHTPKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHL 342

Query: 312 GSSAAS--LYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNV----- 364
            +SA +     +L F++Y+P     PL +Q  +     TN F SP WGG++V+N      
Sbjct: 343 DTSAGASGRSKILQFVVYIPSGKECPLLLQLPNVEISKTNGFISPMWGGVIVWNPGNCDK 402

Query: 365 DSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGP--TSE-GLMTW 421
           DS++ + + + ++   D+ +++E+FL Q R LFG          L +    TSE G   W
Sbjct: 403 DSESPSRNTISLQ---DLEQIVEIFLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEW 459

Query: 422 ELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELAS 481
           ELD L    +  NL + +TTL SL++L+  +  ++IKD++  +V  ++ A + +    + 
Sbjct: 460 ELDVLSRKHTCFNLHSCSTTLGSLSRLVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASL 519

Query: 482 GHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIF 541
           G  +S+  +S+EA + +E AFF PS++ + YF  +  FA+Y P FLP+   ++L+ V+ +
Sbjct: 520 GGYSSSASSSREARSLAENAFFHPSIMSVSYFSYEHCFAVYSPFFLPVVGHVVLAAVREW 579

Query: 542 LETRKSWRK 550
              ++   K
Sbjct: 580 KRYKQEKAK 588
>ref|NP_187374.1| (NM_111598) unknown protein [Arabidopsis thaliana]
 gb|AAF20228.1|AC012395_15 (AC012395) unknown protein [Arabidopsis thaliana]
          Length = 605

 Score =  522 bits (1332), Expect = e-147
 Identities = 111/402 (27%), Positives = 192/402 (47%), Gaps = 31/402 (7%)

Query: 136 EQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRI----VQVAQ 191
           E +    +V V+++ ++    ++ + +G +R A + G    E +  I  R+    VQV  
Sbjct: 200 ENSGKMYSVVVVNKENANGGDEVKAVVGKRRHAWIVGNGLEERYGDIVARVSEIFVQVFM 259

Query: 192 AMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAV 251
                ED +                   P+ S     ++FSLLN +P+     WD +   
Sbjct: 260 NGGREEDSIQGEFM--------------PVGSDGKIVLSFSLLNSNPRDWVYDWDFQRID 305

Query: 252 RRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVINPVESRL 311
              + P   AL    N +V+SQ+LY+        +D    SY      LP  +N  E  L
Sbjct: 306 EALLAPVTKALAPIANITVESQVLYHTPKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHL 365

Query: 312 GSSAAS--LYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNV----- 364
            +SA +     +L F++Y+P     PL +Q  +     TN F SP WGG++V+N      
Sbjct: 366 DTSAGASGRSKILQFVVYIPSGKECPLLLQLPNVEISKTNGFISPMWGGVIVWNPGNCDK 425

Query: 365 DSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGP--TSE-GLMTW 421
           DS++ + + + ++   D+ +++E+FL Q R LFG          L +    TSE G   W
Sbjct: 426 DSESPSRNTISLQ---DLEQIVEIFLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEW 482

Query: 422 ELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELAS 481
           ELD L    +  NL + +TTL SL++L+  +  ++IKD++  +V  ++ A + +    + 
Sbjct: 483 ELDVLSRKHTCFNLHSCSTTLGSLSRLVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASL 542

Query: 482 GHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYI 523
           G  +S+  +S+EA + +E AFF PS++ + YF  +  FA+Y 
Sbjct: 543 GGYSSSASSSREARSLAENAFFHPSIMSVSYFSYEHCFAVYS 584
>gb|AAL27645.1| (AY061834) SD10476p [Drosophila melanogaster]
          Length = 536

 Score =  453 bits (1154), Expect = e-126
 Identities = 111/352 (31%), Positives = 177/352 (49%), Gaps = 16/352 (4%)

Query: 214 SAEKRRPLKSS---LGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSV 270
           S ++R   KS      Y++  S+LNP P+     W+I  AV+ Y++P+L  +    N++V
Sbjct: 185 STDERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTV 244

Query: 271 DSQILYYAMLGVNPRFDSASSS----YYLDMHSLPHVINPVESRLGSSAASLYPVLNFLL 326
            SQ  Y   +  + +     S     Y L   +LPH++  +   L +S     P +N ++
Sbjct: 245 RSQWKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTD-KPAINLVV 303

Query: 327 YVPELAHSPLYIQDKDGAPVATN---AFHSPRWGGIMVYNVDSKTYNASVL---PVRVEV 380
           Y+P    +PL+I +     +  N   AF SP WGG ++ N       A++     V   V
Sbjct: 304 YIPPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHV 363

Query: 381 DMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATT 440
                M+V L QL  L  I+         +          WE +  L   ++ +++TA++
Sbjct: 364 STTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASS 423

Query: 441 TLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEAVTSSEL 500
           TL SL +LL +IS IVI D+V + +  + A +  +   L    LA A V ++ A  +SE 
Sbjct: 424 TLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVASER 483

Query: 501 AFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSL--VKIFLETRKSWRK 550
            FFD SLL  LYFPD+QK+AIYIPLFLP+ VP+L S   ++  L+ R+  ++
Sbjct: 484 GFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSFNMLRGVLQARRKEKQ 535
 Score = 41.3 bits (96), Expect = 0.036
 Identities = 19/49 (38%), Positives = 31/49 (62%)

Query: 19 AALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLRLMVPVTV 67
          A L F  V I +G+P+WW+TT  YR +LP ++I  L+ + ++  V V +
Sbjct: 25 ATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSKIPIKTAVQVAI 73
>gb|AAF56365.1| (AE003749) CG13644 gene product [Drosophila melanogaster]
          Length = 347

 Score =  445 bits (1133), Expect = e-124
 Identities = 109/347 (31%), Positives = 173/347 (49%), Gaps = 16/347 (4%)

Query: 219 RPLKSS---LGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQIL 275
              KS      Y++  S+LNP P+     W+I  AV+ Y++P+L  +    N++V SQ  
Sbjct: 1   MGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWK 60

Query: 276 YYAMLGVNPRFDSASSS----YYLDMHSLPHVINPVESRLGSSAASLYPVLNFLLYVPEL 331
           Y   +  + +     S     Y L   +LPH++  +   L +S     P +N ++Y+P  
Sbjct: 61  YRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTD-KPAINLVVYIPPC 119

Query: 332 AHSPLYIQDKDGAPVATN---AFHSPRWGGIMVYNVDSKTYNASVL---PVRVEVDMVRV 385
             +PL+I +     +  N   AF SP WGG ++ N       A++     V   V     
Sbjct: 120 HIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDN 179

Query: 386 MEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSL 445
           M+V L QL  L  I+         +          WE +  L   ++ +++TA++TL SL
Sbjct: 180 MQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSL 239

Query: 446 AQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEAVTSSELAFFDP 505
            +LL +IS IVI D+V + +  + A +  +   L    LA A V ++ A  +SE  FFD 
Sbjct: 240 IKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGFFDA 299

Query: 506 SLLHLLYFPDDQKFAIYIPLFLPMAVPILLSL--VKIFLETRKSWRK 550
           SLL  LYFPD+QK+AIYIPLFLP+ VP+L S   ++  L+ R+  ++
Sbjct: 300 SLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSFNMLRGVLQARRKEKQ 346
>sp|Q10351|YDA9_SCHPO HYPOTHETICAL 63.9 KD PROTEIN C1F12.09 IN CHROMOSOME I
 pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 pir||T38067 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA93813.1| (Z69944) hypothetical protein [Schizosaccharomyces pombe]
          Length = 554

 Score =  417 bits (1063), Expect = e-115
 Identities = 127/502 (25%), Positives = 220/502 (43%), Gaps = 68/502 (13%)

Query: 61  LMVPV---TVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEE 117
           L +PV   T  + R S+P +D E  P TV            +   RF   + R LD +  
Sbjct: 85  LAIPVWWKTTHYERSSLPFEDMENAPSTV------------QTHLRFSPTF-RILDDKGN 131

Query: 118 ALSSGSVQEAEAMLD-EPQEQA-------EGSLTVYVISEHSSLLP---QDMMSYI---- 162
            L+    +E + +L+ EPQ  +       +  +   ++   S+ L     +    I    
Sbjct: 132 NLT----KEVQKVLEAEPQIYSYNLKVLEDDPVDYRIVLRESTDLQWFWDENNFIIDTPS 187

Query: 163 -GPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPL 221
            GP   A++      EAF+     +       S T              +   AE +R +
Sbjct: 188 KGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSST-------------VEPSRAETKRTV 234

Query: 222 KSSLGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLG 281
           + S  Y +  SLL  +     + WDIE A+++Y  P +  L +    ++++QI Y+    
Sbjct: 235 QFSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVEDA 294

Query: 282 VNPRFDSASSSYYLDMHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDK 341
                D    + + D   LP+++N  E  L  S     P ++F+LYVP     PL+++++
Sbjct: 295 EAYIKDDKFCTKHAD---LPNLVNNFEKYLSFSPHIREPTIHFVLYVPSPQIQPLWLENE 351

Query: 342 DGAPVATNAFHSPRWGGIMVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQ 401
           D   + TN+   P+WG I   N     +N +   +  +VD+     V    L LL GI  
Sbjct: 352 DSNIIPTNSMLLPQWGSITTIN-----FNVTEKKLLHDVDLKDYFRVISRDLLLLLGIND 406

Query: 402 PQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDV 461
               P   LS   +        DRLL  R  E+   A+ TL +LA+L+  + ++ +  ++
Sbjct: 407 ---VPVSSLSATLA--------DRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEI 455

Query: 462 ASEVYKAVAAVQKSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAI 521
              V   + ++  + + L+  +L  A   S  A + S+ A F PS++  +YFPD+ K+ I
Sbjct: 456 QMYVKDTLLSLDMAYKALSQNNLNEALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGI 515

Query: 522 YIPLFLPMAVPILLSLVKIFLE 543
           Y PLF P+ +P+L+S +K   +
Sbjct: 516 YAPLFAPILIPLLISFIKEVKD 537
 Score = 36.3 bits (83), Expect = 1.2
 Identities = 17/35 (48%), Positives = 23/35 (65%)

Query: 17  KRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQI 51
           KR AL    V I+L +P+WWKTT   R+SLP+  +
Sbjct: 72  KRYALLSFYVIILLAIPVWWKTTHYERSSLPFEDM 106
>ref|NP_010722.1| (NC_001136) Glycosyl Phosphatidyl Inositol 17; Gpi17p
           [Saccharomyces cerevisiae]
 pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
 gb|AAB64866.1| (U33007) Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
          Length = 534

 Score =  372 bits (947), Expect = e-102
 Identities = 90/379 (23%), Positives = 153/379 (39%), Gaps = 43/379 (11%)

Query: 190 AQAMSLTEDVLAAALADHLPE--DKWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDI 247
             A +L E         HL +  +  S      +       ++ +LL+ D     V W+I
Sbjct: 152 FVAQTLVEHTFQLEW-THLNKTCEGVSTNNDVAISYDPNIHLSVTLLSGDGN--PVAWEI 208

Query: 248 EGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVINPV 307
           E  +  Y  PF   L    NF+VDS I+Y+  L ++    S +S  + D   L H I+  
Sbjct: 209 EPTLTDYFSPFRKFLSPLVNFTVDSSIVYHNDLNLHSLNGSCTSVTWFD---LSHTIDLS 265

Query: 308 ESRLGSSAASLYP---VLNFLLYVPELAHSPLYIQDKDGAPVAT-------NAFHSPRWG 357
           E     S+ + YP    LN  +  P  + SP  +   +G  ++        N++  P+WG
Sbjct: 266 E----LSSMAYYPEDSALNLAIVFPSASSSPDGLAFINGTRISDEITTLDWNSYLVPQWG 321

Query: 358 GIMVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEG 417
            I++  +  K  N+ +    +E  M R        L L  G +Q  L P           
Sbjct: 322 VIIINKMPLK-PNSVISEDYLEPMMYRFATDIFQLLGLTEG-SQDLLSPYI--------- 370

Query: 418 LMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAE 477
                +D       ++NL  AT TL SL +L  +   + I  +V+  V +A+    +  +
Sbjct: 371 ----TIDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIID 426

Query: 478 EL----ASGHL--ASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAV 531
            L      G +   +A   S E V   E AFF+  ++   +FP +   A+Y+PL  P++ 
Sbjct: 427 LLNDPGKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISA 486

Query: 532 PILLSLVKIFLETRKSWRK 550
            +      +  E  +  +K
Sbjct: 487 VMFFGFYNVMKEKNQKSKK 505
 Score = 39.8 bits (92), Expect = 0.11
 Identities = 23/48 (47%), Positives = 31/48 (63%), Gaps = 3/48 (6%)

Query: 23 FAAVAIVLGLPLWWKTTETYRASLPYSQISGL--NALQ-LRLMVPVTV 67
          F A+ + LG+PLW+K T  YRASLP + I  L  N  Q + L++PV V
Sbjct: 14 FVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYV 61
>ref|XP_091596.1| (XM_091596) similar to phosphatidylinositol glycan, class S (H.
           sapiens) [Homo sapiens]
          Length = 204

 Score =  317 bits (804), Expect = 4e-85
 Identities = 177/178 (99%), Positives = 178/178 (99%)

Query: 97  KMKIKCRFQKAYRRALDHEEEALSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQ 156
           KMKIKCRFQKAYRRALDHEEEALSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQ
Sbjct: 22  KMKIKCRFQKAYRRALDHEEEALSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQ 81

Query: 157 DMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAE 216
           DMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAE
Sbjct: 82  DMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAE 141

Query: 217 KRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQI 274
           KRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQ+
Sbjct: 142 KRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQV 199
>dbj|BAB70938.1| (AK055512) unnamed protein product [Homo sapiens]
          Length = 184

 Score =  262 bits (663), Expect = 1e-68
 Identities = 178/181 (98%), Positives = 179/181 (98%)

Query: 375 PVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVEN 434
           PVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVEN
Sbjct: 4   PVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVEN 63

Query: 435 LATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEA 494
           LATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQE 
Sbjct: 64  LATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEV 123

Query: 495 VTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRKSWRKPEKT 554
           VTSSELAFFDPSLLHLLYFPDDQKF IYIPLFLPMAVPILLSLVK+FLETRKSWRKPEKT
Sbjct: 124 VTSSELAFFDPSLLHLLYFPDDQKFPIYIPLFLPMAVPILLSLVKLFLETRKSWRKPEKT 183

Query: 555 D 555
           D
Sbjct: 184 D 184
>ref|NP_501852.1| (NM_069451) T14G10.7.p [Caenorhabditis elegans]
 pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
 emb|CAA93093.1| (Z68880) predicted using Genefinder~cDNA EST EMBL:M89453 comes from
           this gene~cDNA EST yk111h3.3 comes from this gene~cDNA
           EST yk111h3.5 comes from this gene~cDNA EST yk457b10.3
           comes from this gene~cDNA EST yk457b10.5 comes from this
           gene~cDNA EST yk3>
          Length = 544

 Score =  199 bits (501), Expect = 1e-49
 Identities = 78/292 (26%), Positives = 125/292 (42%), Gaps = 43/292 (14%)

Query: 258 FLNALGAAGNFSVDSQILY-YAMLGVNPRFDSASSSYYLDMHSLPHVINPVESRLGSSAA 316
           F   +    N  V  + L+ +         D     + L   ++  ++  V+S+L +S  
Sbjct: 285 FAEKVKEITNVEVSFEHLWDFEPTRGFLIKD-VQERWALTHSAMEELVKKVDSQLQTS-L 342

Query: 317 SLYPVLNFLLY--VPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDSKTYNASVL 374
               VL F++   V  +       +D  G  VA+       WG ++  N    T + ++ 
Sbjct: 343 QHGAVLRFVVLETVEPVIVLDDTGEDSHGVAVAS-------WGAVLPRN--EATESRAIA 393

Query: 375 PVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVEN 434
            +R+++ M            L  G  +P +             +  WE+ R     SV+N
Sbjct: 394 AIRIQMGM---------DAELSHGWHRPPV------------AICQWEVARARLRASVDN 432

Query: 435 LATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEA 494
              A + + +L QL  KISNIVI DDVA    +A  AV+   + +  G +   F    EA
Sbjct: 433 AMRAASAVRALDQLAQKISNIVINDDVAD---RATRAVEILNDVVRPGRMLD-FERLLEA 488

Query: 495 VTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRK 546
              ++ A  D SLL +LYFP DQ+ A+ IPL    AVPI+L + K+  E  K
Sbjct: 489 RRLADSANSDHSLLAMLYFPMDQRTAVLIPL----AVPIVLPICKLVYEIAK 536
>gb|AAF28987.1|AF161427_1 (AF161427) HSPC309 [Homo sapiens]
          Length = 162

 Score = 80.3 bits (196), Expect = 8e-14
 Identities = 34/58 (58%), Positives = 34/58 (58%), Gaps = 4/58 (6%)

Query: 352 HSPRWGGIMVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCL 409
             P   GIMVYNVDSKTYNASVLPVRVEVDMVRV            G     L P CL
Sbjct: 97  IVPAGVGIMVYNVDSKTYNASVLPVRVEVDMVRVWRCSWHSCGCSLG----LLSPSCL 150
>gb|AAF56366.1| (AE003749) CG6031 gene product [Drosophila melanogaster]
          Length = 164

 Score = 41.7 bits (97), Expect = 0.033
 Identities = 19/49 (38%), Positives = 31/49 (62%)

Query: 19 AALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLRLMVPVTV 67
          A L F  V I +G+P+WW+TT  YR +LP ++I  L+ + ++  V V +
Sbjct: 25 ATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSKIPIKTAVQVAI 73
CPU time:    95.26 user secs.	    2.94 sys. secs	   98.20 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.313    0.156    0.432 

Gapped
Lambda     K      H
   0.270   0.0548    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 249635480
Number of Sequences: 887402
Number of extensions: 9058216
Number of successful extensions: 27652
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 27598
Number of HSP's gapped (non-prelim): 28
length of query: 555
length of database: 277,845,442
effective HSP length: 60
effective length of query: 495
effective length of database: 224,601,322
effective search space: 111177654390
effective search space used: 111177654390
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.2 bits)
S2: 75 (33.4 bits)