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GPI Anchor Biosynthesis Report: NP_010722.1 (PIG-S family, Saccharomyces cerevisiae)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (534 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 15 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_010722.1| (NC_001136) Glycosyl Phosphatidyl Inositol 17; ... 788 0.0
gb|AAL27645.1| (AY061834) SD10476p [Drosophila melanogaster] 617 e-175
sp|Q10351|YDA9_SCHPO HYPOTHETICAL 63.9 KD PROTEIN C1F12.09 IN CH... 572 e-162
ref|NP_149975.1| (NM_033198) phosphatidylinositol glycan, class ... 527 e-148
gb|AAF56365.1| (AE003749) CG13644 gene product [Drosophila melan... 420 e-116
gb|AAL32707.1| (AY062629) Unknown protein [Arabidopsis thaliana] 380 e-104
ref|NP_187374.1| (NM_111598) unknown protein [Arabidopsis thalia... 338 1e-91
dbj|BAB70938.1| (AK055512) unnamed protein product [Homo sapiens] 190 7e-47
ref|XP_091596.1| (XM_091596) similar to phosphatidylinositol gly... 185 2e-45
gb|AAF56366.1| (AE003749) CG6031 gene product [Drosophila melano... 174 4e-42
ref|NP_501852.1| (NM_069451) T14G10.7.p [Caenorhabditis elegans]... 135 2e-30
Sequences with E-value WORSE than threshold

gb|AAF28987.1|AF161427_1 (AF161427) HSPC309 [Homo sapiens] 39 0.29
Alignments
>ref|NP_010722.1| (NC_001136) Glycosyl Phosphatidyl Inositol 17; Gpi17p
           [Saccharomyces cerevisiae]
 pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
 gb|AAB64866.1| (U33007) Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
          Length = 534

 Score =  788 bits (2014), Expect = 0.0
 Identities = 534/534 (100%), Positives = 534/534 (100%)

Query: 1   MSNANLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVY 60
           MSNANLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVY
Sbjct: 1   MSNANLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVY 60

Query: 61  VKSDTYRFPDVHDAIQVQVNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKL 120
           VKSDTYRFPDVHDAIQVQVNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKL
Sbjct: 61  VKSDTYRFPDVHDAIQVQVNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKL 120

Query: 121 DEFIGYSSAYDTKETLVYYDDAAVLSNDLPFFVAQTLVEHTFQLEWTHLNKTCEGVSTNN 180
           DEFIGYSSAYDTKETLVYYDDAAVLSNDLPFFVAQTLVEHTFQLEWTHLNKTCEGVSTNN
Sbjct: 121 DEFIGYSSAYDTKETLVYYDDAAVLSNDLPFFVAQTLVEHTFQLEWTHLNKTCEGVSTNN 180

Query: 181 DVAISYDPNIHLSVTLLSGDGNPVAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIVYHND 240
           DVAISYDPNIHLSVTLLSGDGNPVAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIVYHND
Sbjct: 181 DVAISYDPNIHLSVTLLSGDGNPVAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIVYHND 240

Query: 241 LNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSASSSPDGLAFING 300
           LNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSASSSPDGLAFING
Sbjct: 241 LNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSASSSPDGLAFING 300

Query: 301 TRISDEITTLDWNSYLVPQWGVIIINKMPLKPNSVISEDYLEPMMYRFATDIFQLLGLTE 360
           TRISDEITTLDWNSYLVPQWGVIIINKMPLKPNSVISEDYLEPMMYRFATDIFQLLGLTE
Sbjct: 301 TRISDEITTLDWNSYLVPQWGVIIINKMPLKPNSVISEDYLEPMMYRFATDIFQLLGLTE 360

Query: 361 GSQDLLSPYITIDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDL 420
           GSQDLLSPYITIDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDL
Sbjct: 361 GSQDLLSPYITIDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDL 420

Query: 421 RLQIIDLLNDPGKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPL 480
           RLQIIDLLNDPGKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPL
Sbjct: 421 RLQIIDLLNDPGKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPL 480

Query: 481 LGPISAVMFFGFYNVMKEKNQKSKKNGTEREVAKEKLELKEAQKLHAIDGEDEL 534
           LGPISAVMFFGFYNVMKEKNQKSKKNGTEREVAKEKLELKEAQKLHAIDGEDEL
Sbjct: 481 LGPISAVMFFGFYNVMKEKNQKSKKNGTEREVAKEKLELKEAQKLHAIDGEDEL 534
>gb|AAL27645.1| (AY061834) SD10476p [Drosophila melanogaster]
          Length = 536

 Score =  617 bits (1575), Expect = e-175
 Identities = 116/532 (21%), Positives = 210/532 (38%), Gaps = 60/532 (11%)

Query: 10  VGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYVKSDTYRFP 69
               F+ + + +GVP+W++ TTVYR +LP   I SL           IP+        + 
Sbjct: 25  ATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSK---------IPIKTAVQVAIYT 75

Query: 70  DVHDAIQVQVNHLLNSQEQRVPWSL---QVLPYNETIE-----QMESEGNQFHVVTLKLD 121
                 Q+ +  L N+      WS+   Q+ P   T E      +E    + HV ++   
Sbjct: 76  QQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAALEKLLLENHVQSVGDF 135

Query: 122 EFIGYSSAYDTKETLVYYDDAAVLSNDLPFF-VAQTLVEHTFQLEWTHLNKTCEGVSTNN 180
            FI +      +E L+  + +A++ +D P   +AQ L  H   L+   +N+         
Sbjct: 136 MFIEWPKL--QEELLLTTERSALMRSDTPSNKIAQLL--HAKILQTYRINQILSTDERMG 191

Query: 181 DVAISYDPNIHLSVTLLSGDG--NPVAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIVYH 238
             + +  P   + V++L+         W I   +  Y  P+   +S + N+TV S   Y 
Sbjct: 192 AKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYR 251

Query: 239 -------NDLNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSASSS 291
                    +   S  G   ++    L H +     +  A   +  A+NL +  P    +
Sbjct: 252 VAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCHIA 311

Query: 292 PDGLAFINGTRISDEITTLDWNSYLVPQWGVIIINKMPLK------------PNSVISED 339
           P  L   N       +T  D ++++ P WG  II   P              P  V + D
Sbjct: 312 P--LHIYNSK--DQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTD 367

Query: 340 YLEPMMYRFATDI-----FQLLGLTEGSQDLLSP-YITIDSFKRLTILQNLDKATETLWS 393
            ++ M+ +    +      Q+ G+     + L P     +++ R + ++++  A+ TL S
Sbjct: 368 NMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQS 427

Query: 394 LVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNELVKLC 453
           L+KL  Q   + I  EV   +  +        D+L       +    +A  L+       
Sbjct: 428 LIKLLDQISYIVIDDEVGAAITNSY------ADILAAKAALLEHRLADASVLAKRAFVAS 481

Query: 454 EKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSKK 505
           E+ FF+  ++ Q +FP E   A+Y+PL  PI   +   F N+++   Q  +K
Sbjct: 482 ERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSF-NMLRGVLQARRK 532
>sp|Q10351|YDA9_SCHPO HYPOTHETICAL 63.9 KD PROTEIN C1F12.09 IN CHROMOSOME I
 pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 pir||T38067 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA93813.1| (Z69944) hypothetical protein [Schizosaccharomyces pombe]
          Length = 554

 Score =  572 bits (1461), Expect = e-162
 Identities = 113/513 (22%), Positives = 215/513 (41%), Gaps = 52/513 (10%)

Query: 5   NLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYVKSD 64
           +L+++    F  I L L +P+W+K T   R+SLP   +E+  +      HL       S 
Sbjct: 70  SLKRYALLSFYVIIL-LAIPVWWKTTHYERSSLPFEDMENAPST--VQTHLRF-----SP 121

Query: 65  TYR-FPDVHDAIQVQVNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKLDEF 123
           T+R   D  + +  +V  +L ++ Q   ++L+VL         E +   + +V   L E 
Sbjct: 122 TFRILDDKGNNLTKEVQKVLEAEPQIYSYNLKVL---------EDDPVDYRIV---LRES 169

Query: 124 IGYSSAYDTKETLVYYDDAAVLSNDLPFFVAQTLVE------HTFQLEWTHLNKTCEGVS 177
                 +D    ++  D  +   ++L   +   L E           ++T  + T E   
Sbjct: 170 TDLQWFWDENNFII--DTPSKGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSSTVEPSR 227

Query: 178 TNNDVAISYDPNIHLSVTLLSGDGN--PVAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSI 235
                 + + P   + ++LL G+GN  P+ W+IE  +  YF+P  + L+ L    +++ I
Sbjct: 228 AETKRTVQFSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQI 287

Query: 236 VYHNDLNLHSLNGSCTSVTWFDLSHTI-DLSELSSMAYYPEDSALNLAIVFPSASSSPDG 294
            Y  +     +          DL + + +  +  S + +  +  ++  +  PS    P  
Sbjct: 288 QYFVEDAEAYIKDDKFCTKHADLPNLVNNFEKYLSFSPHIREPTIHFVLYVPSPQIQPLW 347

Query: 295 LAFINGTRISDEITTLDWNSYLVPQWGVIIINKMPLKPNSVISEDYLEPMMYRFATDIFQ 354
           L        +++   +  NS L+PQWG I      +    ++ +  L+      + D+  
Sbjct: 348 LE-------NEDSNIIPTNSMLLPQWGSITTINFNVTEKKLLHDVDLKDYFRVISRDLLL 400

Query: 355 LLGLTEGSQDLLSPYITIDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVS--- 411
           LLG+ +     LS  +  D   R  I ++  +A++TL +L KL    Q M++P+E+    
Sbjct: 401 LLGINDVPVSSLSATLA-DRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYV 459

Query: 412 DNVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQE 471
            + + +LD+  + +   N          N AL  SN      ++A F+  MV   +FP E
Sbjct: 460 KDTLLSLDMAYKALSQNN---------LNEALSYSNNAFSKSQEALFHPSMVTTIYFPDE 510

Query: 472 HMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSK 504
               +Y PL  PI   +   F   +K+  ++ K
Sbjct: 511 SKYGIYAPLFAPILIPLLISFIKEVKDMLRERK 543
>ref|NP_149975.1| (NM_033198) phosphatidylinositol glycan, class S [Homo sapiens]
 dbj|BAB60853.1| (AB057723) phosphatidyl inositol glycan class S [Homo sapiens]
          Length = 555

 Score =  527 bits (1343), Expect = e-148
 Identities = 119/574 (20%), Positives = 204/574 (34%), Gaps = 116/574 (20%)

Query: 8   KWVGFCFVAIYLFLGVPLWYKLT----------------------------TVYRASLPI 39
           K     F A+ + LG+PLW+K T                               R S+P+
Sbjct: 17  KRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLRLMVPVTVVFTRESVPL 76

Query: 40  NYIESLQNNKFQDIHLV-IPVYVKSDTYRFPDVHDAIQVQVNHLLNSQEQRVPWSLQVLP 98
           +  E L    F  +H   IP+  K             Q      L+ +E+ +        
Sbjct: 77  DDQEKLP---FTVVHEREIPLKYKMKI------KCRFQKAYRRALDHEEEALS-----SG 122

Query: 99  YNETIEQMESEGNQFHVVTLKLDEFIGYSSAYDTKET-LVYYDDAAVLSNDL---PFFV- 153
             +  E M  E  +    +L +     +SS         +     AV+   +    F + 
Sbjct: 123 SVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNII 182

Query: 154 ------------------AQTLVEHTFQLEWTHLNKTCEGVSTNNDVAISYDPNIHLSVT 195
                             A  L +H  + +W          S      +       ++ +
Sbjct: 183 GRRIVQVAQAMSLTEDVLAAALADHLPEDKW----------SAEKRRPLKSSLGYEITFS 232

Query: 196 LLSGDG--NPVAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIVYHNDLNLHSLNGSCTSV 253
           LL+ D   + V W+IE  +  Y  PF   L    NF+VDS I+Y+  L ++    S +S 
Sbjct: 233 LLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSS 292

Query: 254 TWFD---LSHTIDLSE----LSSMAYYPEDSALNLAIVFPSASSSPDGLAFINGTRISDE 306
            + D   L H I+  E     S+ + Y     LN  +  P  + SP  +   +G  ++  
Sbjct: 293 YYLDMHSLPHVINPVESRLGSSAASLY---PVLNFLLYVPELAHSPLYIQDKDGAPVA-- 347

Query: 307 ITTLDWNSYLVPQWGVIIINKMPLKPNS-VISEDYLEPMMYRFATDIFQLLGLTEG-SQD 364
                 N++  P+WG I++  +  K  +  +    +E  M R        L L  G +Q 
Sbjct: 348 -----TNAFHSPRWGGIMVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQP 402

Query: 365 LLSPY-------------ITIDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVS 411
            L P                +D       ++NL  AT TL SL +L  +   + I  +V+
Sbjct: 403 QLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVA 462

Query: 412 DNVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQE 471
             V +A+    +  + L      G     +A   S E V   E AFF+  ++   +FP +
Sbjct: 463 SEVYKAVAAVQKSAEEL----ASGH--LASAFVASQEAVTSSELAFFDPSLLHLLYFPDD 516

Query: 472 HMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSKK 505
              A+Y+PL  P++  +      +  E  +  +K
Sbjct: 517 QKFAIYIPLFLPMAVPILLSLVKIFLETRKSWRK 550
>gb|AAF56365.1| (AE003749) CG13644 gene product [Drosophila melanogaster]
          Length = 347

 Score =  420 bits (1070), Expect = e-116
 Identities = 74/354 (20%), Positives = 137/354 (37%), Gaps = 38/354 (10%)

Query: 179 NNDVAISYDPNIHLSVTLLSGDG--NPVAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIV 236
               + +  P   + V++L+         W I   +  Y  P+   +S + N+TV S   
Sbjct: 1   MGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWK 60

Query: 237 YH-------NDLNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSAS 289
           Y          +   S  G   ++    L H +     +  A   +  A+NL +  P   
Sbjct: 61  YRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCH 120

Query: 290 SSPDGLAFINGTRISDEITTLDWNSYLVPQWGVIIINKMPLK------------PNSVIS 337
            +P  L   N       +T  D ++++ P WG  II   P              P  V +
Sbjct: 121 IAP--LHIYNSK--DQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVST 176

Query: 338 EDYLEPMMYRFATDI-----FQLLGLTEGSQDLLSP-YITIDSFKRLTILQNLDKATETL 391
            D ++ M+ +    +      Q+ G+     + L P     +++ R + ++++  A+ TL
Sbjct: 177 TDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTL 236

Query: 392 WSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNELVK 451
            SL+KL  Q   + I  EV   +  +        D+L       +    +A  L+     
Sbjct: 237 QSLIKLLDQISYIVIDDEVGAAITNSY------ADILAAKAALLEHRLADASVLAKRAFV 290

Query: 452 LCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSKK 505
             E+ FF+  ++ Q +FP E   A+Y+PL  PI   +   F N+++   Q  +K
Sbjct: 291 ASERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSF-NMLRGVLQARRK 343
>gb|AAL32707.1| (AY062629) Unknown protein [Arabidopsis thaliana]
          Length = 599

 Score =  380 bits (968), Expect = e-104
 Identities = 84/354 (23%), Positives = 135/354 (37%), Gaps = 47/354 (13%)

Query: 183 AISYDPNIHLSVTLLSGDGNP--VAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIVYHN- 239
            +  D  I LS +LL+ +       W+ +       +P  K L+P+ N TV+S ++YH  
Sbjct: 251 PVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTP 310

Query: 240 -------DLNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSASSSP 292
                  D  L S     + + +F  S+   L   +S         L   +  PS    P
Sbjct: 311 KSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLD--TSAGASGRSKILQFVVYIPSGKECP 368

Query: 293 DGLAFINGTRISDEITTLDWNSYLVPQWGVIII------NKMPLKPN-SVISEDYLEPMM 345
             L   N   IS        N ++ P WG +I+      +K    P+ + IS   LE ++
Sbjct: 369 LLLQLPN-VEISK------TNGFISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIV 421

Query: 346 YRFATDIFQLLGLTE--------GSQDLLSP-----YITIDSFKRLTILQNLDKATETLW 392
             F     QL G           G+  +L+         +D   R     NL   + TL 
Sbjct: 422 EIFLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLG 481

Query: 393 SLVKLTQQFQGMSIPRE-VSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNELVK 451
           SL +L +     S+PR  + D + E +   L+   L       G   ++++   S E   
Sbjct: 482 SLSRLVR-----SLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGG--YSSSASSSREARS 534

Query: 452 LCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSKK 505
           L E AFF+  ++  ++F  EH  AVY P   P+   +        K   Q+  K
Sbjct: 535 LAENAFFHPSIMSVSYFSYEHCFAVYSPFFLPVVGHVVLAAVREWKRYKQEKAK 588
 Score = 43.2 bits (101), Expect = 0.012
 Identities = 24/104 (23%), Positives = 42/104 (40%), Gaps = 6/104 (5%)

Query: 6   LRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIP-----VY 60
           L++      V     LGVP  +K   +YR+ LP + I+SL  ++ +   L  P     V+
Sbjct: 28  LKRLFITSSVLFSFLLGVPFLWKSVEIYRSPLPFHDIDSLS-DQLESTPLRFPCNFHAVF 86

Query: 61  VKSDTYRFPDVHDAIQVQVNHLLNSQEQRVPWSLQVLPYNETIE 104
           V   +    ++   IQ  +N L +   Q       +    +  E
Sbjct: 87  VGFRSTDPDNLRSQIQDGINQLTHQSSQCGSCDFSLSVTVQNRE 130
>ref|NP_187374.1| (NM_111598) unknown protein [Arabidopsis thaliana]
 gb|AAF20228.1|AC012395_15 (AC012395) unknown protein [Arabidopsis thaliana]
          Length = 605

 Score =  338 bits (860), Expect = 1e-91
 Identities = 79/327 (24%), Positives = 127/327 (38%), Gaps = 47/327 (14%)

Query: 183 AISYDPNIHLSVTLLSGDGNP--VAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIVYHN- 239
            +  D  I LS +LL+ +       W+ +       +P  K L+P+ N TV+S ++YH  
Sbjct: 274 PVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTP 333

Query: 240 -------DLNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSASSSP 292
                  D  L S     + + +F  S+   L   +S         L   +  PS    P
Sbjct: 334 KSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLD--TSAGASGRSKILQFVVYIPSGKECP 391

Query: 293 DGLAFINGTRISDEITTLDWNSYLVPQWGVIII------NKMPLKPN-SVISEDYLEPMM 345
             L   N   IS        N ++ P WG +I+      +K    P+ + IS   LE ++
Sbjct: 392 LLLQLPN-VEISK------TNGFISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIV 444

Query: 346 YRFATDIFQLLGLTE--------GSQDLLSP-----YITIDSFKRLTILQNLDKATETLW 392
             F     QL G           G+  +L+         +D   R     NL   + TL 
Sbjct: 445 EIFLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLG 504

Query: 393 SLVKLTQQFQGMSIPRE-VSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNELVK 451
           SL +L +     S+PR  + D + E +   L+   L       G   ++++   S E   
Sbjct: 505 SLSRLVR-----SLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGG--YSSSASSSREARS 557

Query: 452 LCEKAFFNGEMVQQNFFPQEHMIAVYL 478
           L E AFF+  ++  ++F  EH  AVY 
Sbjct: 558 LAENAFFHPSIMSVSYFSYEHCFAVYS 584
 Score = 41.3 bits (96), Expect = 0.044
 Identities = 21/88 (23%), Positives = 37/88 (41%), Gaps = 6/88 (6%)

Query: 22  GVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIP-----VYVKSDTYRFPDVHDAIQ 76
           GVP  +K   +YR+ LP + I+SL  ++ +   L  P     V+V   +    ++   IQ
Sbjct: 67  GVPFLWKSVEIYRSPLPFHDIDSLS-DQLESTPLRFPCNFHAVFVGFRSTDPDNLRSQIQ 125

Query: 77  VQVNHLLNSQEQRVPWSLQVLPYNETIE 104
             +N L +   Q       +    +  E
Sbjct: 126 DGINQLTHQSSQCGSCDFSLSVTVQNRE 153
>dbj|BAB70938.1| (AK055512) unnamed protein product [Homo sapiens]
          Length = 184

 Score =  190 bits (478), Expect = 7e-47
 Identities = 41/182 (22%), Positives = 70/182 (37%), Gaps = 20/182 (10%)

Query: 338 EDYLEPMMYRFATDIFQLLGLTEG-SQDLLSPY-------------ITIDSFKRLTILQN 383
              +E  M R        L L  G +Q  L P                +D       ++N
Sbjct: 4   PVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVEN 63

Query: 384 LDKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNAL 443
           L  AT TL SL +L  +   + I  +V+  V +A+    +  + L      G     +A 
Sbjct: 64  LATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEEL----ASGH--LASAF 117

Query: 444 HLSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMKEKNQKS 503
             S E+V   E AFF+  ++   +FP +    +Y+PL  P++  +      +  E  +  
Sbjct: 118 VASQEVVTSSELAFFDPSLLHLLYFPDDQKFPIYIPLFLPMAVPILLSLVKLFLETRKSW 177

Query: 504 KK 505
           +K
Sbjct: 178 RK 179
>ref|XP_091596.1| (XM_091596) similar to phosphatidylinositol glycan, class S (H.
           sapiens) [Homo sapiens]
          Length = 204

 Score =  185 bits (466), Expect = 2e-45
 Identities = 33/189 (17%), Positives = 59/189 (30%), Gaps = 40/189 (21%)

Query: 72  HDAIQVQVNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKLDEFIGYSSAYD 131
               Q      L+ +E+ +          +  E M  E  +    +L +     +SS   
Sbjct: 26  KCRFQKAYRRALDHEEEALS-----SGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLP 80

Query: 132 TKET-LVYYDDAAVLSNDL---PFFV-------------------AQTLVEHTFQLEWTH 168
                 +     AV+   +    F +                   A  L +H  + +W  
Sbjct: 81  QDMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKW-- 138

Query: 169 LNKTCEGVSTNNDVAISYDPNIHLSVTLLSGDG--NPVAWEIEPTLTDYFSPFRKFLSPL 226
                   S      +       ++ +LL+ D   + V W+IE  +  Y  PF   L   
Sbjct: 139 --------SAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAA 190

Query: 227 VNFTVDSSI 235
            NF+VDS +
Sbjct: 191 GNFSVDSQV 199
>gb|AAF56366.1| (AE003749) CG6031 gene product [Drosophila melanogaster]
          Length = 164

 Score =  174 bits (438), Expect = 4e-42
 Identities = 35/148 (23%), Positives = 62/148 (41%), Gaps = 19/148 (12%)

Query: 10  VGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYVKSDTYRFP 69
               F+ + + +GVP+W++ TTVYR +LP   I SL           IP+        + 
Sbjct: 25  ATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSK---------IPIKTAVQVAIYT 75

Query: 70  DVHDAIQVQVNHLLNSQEQRVPWSL---QVLPYNETIE-----QMESEGNQFHVVTLKLD 121
                 Q+ +  L N+      WS+   Q+ P   T E      +E    + HV ++   
Sbjct: 76  QQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAALEKLLLENHVQSVGDF 135

Query: 122 EFIGYSSAYDTKETLVYYDDAAVLSNDL 149
            FI +      +E L+  + +A++ +D 
Sbjct: 136 MFIEWPKL--QEELLLTTERSALMRSDT 161
>ref|NP_501852.1| (NM_069451) T14G10.7.p [Caenorhabditis elegans]
 pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
 emb|CAA93093.1| (Z68880) predicted using Genefinder~cDNA EST EMBL:M89453 comes from
           this gene~cDNA EST yk111h3.3 comes from this gene~cDNA
           EST yk111h3.5 comes from this gene~cDNA EST yk457b10.3
           comes from this gene~cDNA EST yk457b10.5 comes from this
           gene~cDNA EST yk3>
          Length = 544

 Score =  135 bits (337), Expect = 2e-30
 Identities = 37/237 (15%), Positives = 82/237 (33%), Gaps = 30/237 (12%)

Query: 252 SVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSASSSPDGLAFINGTRISDEITTLD 311
           ++T   +   +   +          + L   +           L  +    + D+ T  D
Sbjct: 321 ALTHSAMEELVKKVDSQLQTSLQHGAVLRFVV-----------LETVEPVIVLDD-TGED 368

Query: 312 WNSYLVPQWGVIIINKMPLKPNSVISEDYLEPMMYRFATDIFQLLGLTEGSQDLLSPYIT 371
            +   V  WG ++        +  I+   ++  M    +      G       +      
Sbjct: 369 SHGVAVASWGAVLPRN-EATESRAIAAIRIQMGMDAELSH-----GWHRPPVAIC--QWE 420

Query: 372 IDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDP 431
           +   +    + N  +A   + +L +L Q+   + I  +V+D    A+       ++LND 
Sbjct: 421 VARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVADRATRAV-------EILNDV 473

Query: 432 GKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVM 488
            + G ++    L    E  +L + A  +  ++   +FP +   AV +PL  PI   +
Sbjct: 474 VRPGRMLDFERLL---EARRLADSANSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPI 527
 Score = 39.7 bits (92), Expect = 0.12
 Identities = 15/71 (21%), Positives = 34/71 (47%), Gaps = 5/71 (7%)

Query: 10  VGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYVKSDTYRFP 69
               FV +  F+G+P+W+  T+ YR++ P     +  +N+   + + +   + S+  R  
Sbjct: 48  ASLSFVILVFFIGMPMWHFTTSTYRSNFP-----TFPSNQSITVPIRVIFVITSEDLRND 102

Query: 70  DVHDAIQVQVN 80
                 +++VN
Sbjct: 103 VKTLMNELEVN 113
>gb|AAF28987.1|AF161427_1 (AF161427) HSPC309 [Homo sapiens]
          Length = 162

 Score = 38.6 bits (89), Expect = 0.29
 Identities = 20/139 (14%), Positives = 34/139 (24%), Gaps = 16/139 (11%)

Query: 217 SPFRKFLSPLVNFTVDSSIVYHNDLNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPED 276
            PF   L         S  +  N           T +       TI     S M+     
Sbjct: 1   QPFLNALGAAELLCGLSDSLLCNVGGESPRL---TQLP----PATIWTCTASPMSSTQWS 53

Query: 277 SALNLAIVFPSASSSP------DGLAFINGTRISDEITTLDWNSYLVPQWGVIIINKMPL 330
              +  +  P  +           L     T  +           +VP    I++  +  
Sbjct: 54  PGWDPVL--PPCTLCSTFYSTCLSLHTHRCTFRTRMALQWPPMPSIVPAGVGIMVYNVDS 111

Query: 331 KPNS-VISEDYLEPMMYRF 348
           K  +  +    +E  M R 
Sbjct: 112 KTYNASVLPVRVEVDMVRV 130
CPU time:    87.43 user secs.	    1.92 sys. secs	   89.35 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.311    0.160    0.426 

Gapped
Lambda     K      H
   0.270   0.0561    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 235530429
Number of Sequences: 887402
Number of extensions: 8386508
Number of successful extensions: 29871
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 29781
Number of HSP's gapped (non-prelim): 30
length of query: 534
length of database: 277,845,442
effective HSP length: 58
effective length of query: 476
effective length of database: 226,376,126
effective search space: 107755035976
effective search space used: 107755035976
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.0 bits)
S2: 75 (33.4 bits)