IMP GPI Biosynthesis Report IMP-Bioinformatics
GPI Biosynthesis Main Page  
GPI Site Motif   GPI Site Prediction   Home Page B.E.  

GPI Anchor Biosynthesis Report: BAB92563.1 (PIG-S family, Oryza sativa (japonica cultivar-group))




BLASTP 2.1.1 [Aug-8-2000]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (557 letters)

Database: nr
           1,315,992 sequences; 421,297,171 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 18 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

dbj|BAB92563.1| P0497A05.6 [Oryza sativa (japonica cultivar-group)] 877 0.0
ref|NP_187374.2| unknown protein; protein id: At3g07180.1, suppo... 732 0.0
gb|AAF20228.1|AC012395_15 unknown protein [Arabidopsis thaliana] 705 0.0
ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapie... 449 e-124
dbj|BAC11615.1| unnamed protein product [Homo sapiens] 445 e-123
ref|XP_203412.1| similar to phosphatidylinositol glycan class S ... 440 e-122
ref|XP_220633.1| similar to phosphatidylinositol glycan class S ... 432 e-119
ref|NP_733060.1| CG31120-PB [Drosophila melanogaster] >gi|169020... 359 1e-97
gb|EAA07616.1| agCP1277 [Anopheles gambiae str. PEST] 349 8e-95
ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe... 349 1e-94
ref|XP_142436.2| similar to phosphatidylinositol glycan class S ... 302 2e-80
ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Sacc... 262 1e-68
dbj|BAB70938.1| unnamed protein product [Homo sapiens] 141 4e-32
ref|XP_194600.1| similar to phosphatidylinositol glycan class S ... 71 6e-11
Sequences with E-value WORSE than threshold

ref|NP_501852.1| Putative endoplasmic reticulum membrane protein... 41 0.056
Alignments
>dbj|BAB92563.1| P0497A05.6 [Oryza sativa (japonica cultivar-group)]
          Length = 557

 Score =  877 bits (2243), Expect = 0.0
 Identities = 557/557 (100%), Positives = 557/557 (100%)

Query: 1   MAVLAEIAGDESPPPLPPSEGGEASGAPSTSSPSDADGGGKPSPRTSKPGRKRLVLTASV 60
           MAVLAEIAGDESPPPLPPSEGGEASGAPSTSSPSDADGGGKPSPRTSKPGRKRLVLTASV
Sbjct: 1   MAVLAEIAGDESPPPLPPSEGGEASGAPSTSSPSDADGGGKPSPRTSKPGRKRLVLTASV 60

Query: 61  LLSFLIGLPLLLKSTEIHRSPLPSDAIAALSRRLHSNPPSFPCGLHAVFLRSGRDPSEAS 120
           LLSFLIGLPLLLKSTEIHRSPLPSDAIAALSRRLHSNPPSFPCGLHAVFLRSGRDPSEAS
Sbjct: 61  LLSFLIGLPLLLKSTEIHRSPLPSDAIAALSRRLHSNPPSFPCGLHAVFLRSGRDPSEAS 120

Query: 121 VANRIEREISTQLVDLPDASTAGKISVSVTVESAGGCSSSSKVASPWRCGAVTTADLGRG 180
           VANRIEREISTQLVDLPDASTAGKISVSVTVESAGGCSSSSKVASPWRCGAVTTADLGRG
Sbjct: 121 VANRIEREISTQLVDLPDASTAGKISVSVTVESAGGCSSSSKVASPWRCGAVTTADLGRG 180

Query: 181 DEVFDELLDSALGDGGGDGMRVYTVVFVDSDDLKRIVIGKHRHAWVVGKVDEAEVVSIIG 240
           DEVFDELLDSALGDGGGDGMRVYTVVFVDSDDLKRIVIGKHRHAWVVGKVDEAEVVSIIG
Sbjct: 181 DEVFDELLDSALGDGGGDGMRVYTVVFVDSDDLKRIVIGKHRHAWVVGKVDEAEVVSIIG 240

Query: 241 KVFVKYFMNGGVEEGEASTVKREFMPVGSDGNIVLSFSLLNADPSDWVYDWEFENIGQRM 300
           KVFVKYFMNGGVEEGEASTVKREFMPVGSDGNIVLSFSLLNADPSDWVYDWEFENIGQRM
Sbjct: 241 KVFVKYFMNGGVEEGEASTVKREFMPVGSDGNIVLSFSLLNADPSDWVYDWEFENIGQRM 300

Query: 301 LTPVIEALRPIANINIESQVLYHTPKSSYSYSDDKLGGNVLSVGDIPFFVNSNEWHLDTS 360
           LTPVIEALRPIANINIESQVLYHTPKSSYSYSDDKLGGNVLSVGDIPFFVNSNEWHLDTS
Sbjct: 301 LTPVIEALRPIANINIESQVLYHTPKSSYSYSDDKLGGNVLSVGDIPFFVNSNEWHLDTS 360

Query: 361 ISATGRSKVLQFVVYIPSARECPLYLQLPDGELSKTNAFISPMWGGVVIWNPPGCSFGSK 420
           ISATGRSKVLQFVVYIPSARECPLYLQLPDGELSKTNAFISPMWGGVVIWNPPGCSFGSK
Sbjct: 361 ISATGRSKVLQFVVYIPSARECPLYLQLPDGELSKTNAFISPMWGGVVIWNPPGCSFGSK 420

Query: 421 PHGALDKMSSEELMETIEIFIGQLRQLFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLL 480
           PHGALDKMSSEELMETIEIFIGQLRQLFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLL
Sbjct: 421 PHGALDKMSSEELMETIEIFIGQLRQLFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLL 480

Query: 481 YRHHACSNLLSCLTTLESLSSLVQSLPRMIVMDEIGRQPFFAPVSLHVLLAVMKELKRYM 540
           YRHHACSNLLSCLTTLESLSSLVQSLPRMIVMDEIGRQPFFAPVSLHVLLAVMKELKRYM
Sbjct: 481 YRHHACSNLLSCLTTLESLSSLVQSLPRMIVMDEIGRQPFFAPVSLHVLLAVMKELKRYM 540

Query: 541 VERRKYSAFLASQATSS 557
           VERRKYSAFLASQATSS
Sbjct: 541 VERRKYSAFLASQATSS 557
>ref|NP_187374.2| unknown protein; protein id: At3g07180.1, supported by cDNA:
           gi_17065105 [Arabidopsis thaliana]
 gb|AAL32707.1| Unknown protein [Arabidopsis thaliana]
 gb|AAM91117.1| unknown protein [Arabidopsis thaliana]
          Length = 599

 Score =  732 bits (1870), Expect = 0.0
 Identities = 244/498 (48%), Positives = 325/498 (64%), Gaps = 19/498 (3%)

Query: 45  RTSKPGRKRLVLTASVLLSFLIGLPLLLKSTEIHRSPLPSDAIAALSRRLHSNPPSFPCG 104
           R++KPG KRL +T+SVL SFL+G+P L KS EI+RSPLP   I +LS +L S P  FPC 
Sbjct: 22  RSTKPGLKRLFITSSVLFSFLLGVPFLWKSVEIYRSPLPFHDIDSLSDQLESTPLRFPCN 81

Query: 105 LHAVFLRSGRDPSEASVANRIEREISTQLVDLPDASTAGKISVSVTVES-AGGCSSS--- 160
            HAVF+   R     ++ ++I+  I+ QL        +   S+SVTV++    CS +   
Sbjct: 82  FHAVFV-GFRSTDPDNLRSQIQDGIN-QLTHQSSQCGSCDFSLSVTVQNREDQCSDTLAH 139

Query: 161 SKVASPWRCGAVTTADLGRG-DEVFDELLDSALGDGGGDGMRVYTVVFVDSD-----DLK 214
           S     +RCG +       G D+  DE L+        +  ++Y+VV V+ +     D  
Sbjct: 140 SSTTCSYRCGVIKRNGFSVGLDDTVDESLNDVFSGCSENSGKMYSVVVVNKENANGGDEV 199

Query: 215 RIVIGKHRHAWVVGKVDE---AEVVSIIGKVFVKYFMNGGVEEGEASTVKREFMPVGSDG 271
           + V+GK RHAW+VG   E    ++V+ + ++FV+ FMNGG EE    +++ EFMPVGSDG
Sbjct: 200 KAVVGKRRHAWIVGNGLEERYGDIVARVSEIFVQVFMNGGREE---DSIQGEFMPVGSDG 256

Query: 272 NIVLSFSLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQVLYHTPKSSYSY 331
            IVLSFSLLN++P DWVYDW+F+ I + +L PV +AL PIANI +ESQVLYHTPKSS+S 
Sbjct: 257 KIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSSFSS 316

Query: 332 SDDKLGGNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYIPSARECPLYLQLPDG 391
            D KL   +    D+PFFVNSNEWHLDTS  A+GRSK+LQFVVYIPS +ECPL LQLP+ 
Sbjct: 317 WDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLLQLPNV 376

Query: 392 ELSKTNAFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSEELMETIEIFIGQLRQLFGLK 451
           E+SKTN FISPMWGGV++WNP  C        + + +S ++L + +EIF+GQ RQLFG K
Sbjct: 377 EISKTNGFISPMWGGVIVWNPGNCDKD-SESPSRNTISLQDLEQIVEIFLGQFRQLFGFK 435

Query: 452 SSYHTQSMDGVTKFITSPKGFAQWELDLLYRHHACSNLLSCLTTLESLSSLVQSLPRMIV 511
           S     +  G  K +TS +GF +WELD+L R H C NL SC TTL SLS LV+SLPRMI+
Sbjct: 436 SEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSRLVRSLPRMII 495

Query: 512 MDEIGRQPFFAPVSLHVL 529
            DEIG Q  ++  +  + 
Sbjct: 496 KDEIGEQVKYSLKAAKLA 513
>gb|AAF20228.1|AC012395_15 unknown protein [Arabidopsis thaliana]
          Length = 605

 Score =  705 bits (1802), Expect = 0.0
 Identities = 242/521 (46%), Positives = 323/521 (61%), Gaps = 42/521 (8%)

Query: 45  RTSKPGRKRLVLTASVLLSFLI-----------------------GLPLLLKSTEIHRSP 81
           R++KPG KRL +T+SVL   L+                       G+P L KS EI+RSP
Sbjct: 22  RSTKPGLKRLFITSSVLFFGLLNSLSSEFSISLPLLYPFPVSEVSGVPFLWKSVEIYRSP 81

Query: 82  LPSDAIAALSRRLHSNPPSFPCGLHAVFLRSGRDPSEASVANRIEREISTQLVDLPDAST 141
           LP   I +LS +L S P  FPC  HAVF+   R     ++ ++I+  I+ QL        
Sbjct: 82  LPFHDIDSLSDQLESTPLRFPCNFHAVFV-GFRSTDPDNLRSQIQDGIN-QLTHQSSQCG 139

Query: 142 AGKISVSVTVES-AGGCSSS---SKVASPWRCGAVTTADLGRG-DEVFDELLDSALGDGG 196
           +   S+SVTV++    CS +   S     +RCG +       G D+  DE L+       
Sbjct: 140 SCDFSLSVTVQNREDQCSDTLAHSSTTCSYRCGVIKRNGFSVGLDDTVDESLNDVFSGCS 199

Query: 197 GDGMRVYTVVFVDSD-----DLKRIVIGKHRHAWVVGKVDE---AEVVSIIGKVFVKYFM 248
            +  ++Y+VV V+ +     D  + V+GK RHAW+VG   E    ++V+ + ++FV+ FM
Sbjct: 200 ENSGKMYSVVVVNKENANGGDEVKAVVGKRRHAWIVGNGLEERYGDIVARVSEIFVQVFM 259

Query: 249 NGGVEEGEASTVKREFMPVGSDGNIVLSFSLLNADPSDWVYDWEFENIGQRMLTPVIEAL 308
           NGG EE    +++ EFMPVGSDG IVLSFSLLN++P DWVYDW+F+ I + +L PV +AL
Sbjct: 260 NGGREE---DSIQGEFMPVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKAL 316

Query: 309 RPIANINIESQVLYHTPKSSYSYSDDKLGGNVLSVGDIPFFVNSNEWHLDTSISATGRSK 368
            PIANI +ESQVLYHTPKSS+S  D KL   +    D+PFFVNSNEWHLDTS  A+GRSK
Sbjct: 317 APIANITVESQVLYHTPKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSK 376

Query: 369 VLQFVVYIPSARECPLYLQLPDGELSKTNAFISPMWGGVVIWNPPGCSFGSKPHGALDKM 428
           +LQFVVYIPS +ECPL LQLP+ E+SKTN FISPMWGGV++WNP  C        + + +
Sbjct: 377 ILQFVVYIPSGKECPLLLQLPNVEISKTNGFISPMWGGVIVWNPGNCDKD-SESPSRNTI 435

Query: 429 SSEELMETIEIFIGQLRQLFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLLYRHHACSN 488
           S ++L + +EIF+GQ RQLFG KS     +  G  K +TS +GF +WELD+L R H C N
Sbjct: 436 SLQDLEQIVEIFLGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFN 495

Query: 489 LLSCLTTLESLSSLVQSLPRMIVMDEIGRQPFFAPVSLHVL 529
           L SC TTL SLS LV+SLPRMI+ DEIG Q  ++  +  + 
Sbjct: 496 LHSCSTTLGSLSRLVRSLPRMIIKDEIGEQVKYSLKAAKLA 536
>ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapiens]
 sp|Q96S52|PIGS_HUMAN GPI transamidase component PIG-S (Phosphatidylinositol-glycan
           biosynthesis, class S protein)
 dbj|BAB60853.1| phosphatidyl inositol glycan class S [Homo sapiens]
          Length = 555

 Score =  449 bits (1143), Expect = e-124
 Identities = 92/338 (27%), Positives = 159/338 (46%), Gaps = 25/338 (7%)

Query: 221 HRHAW-VVGK--VDEAEVVSIIGKVFVKYFMNGGVEEGEASTVKREFMPVGSDGNIVLSF 277
           HR A+ ++G+  V  A+ +S+   V      +   E+  ++  +R   P+ S     ++F
Sbjct: 175 HREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRR---PLKSSLGYEITF 231

Query: 278 SLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQVLYHTPKSSYSYSDDKLG 337
           SLLN DP      W+ E   +R + P + AL    N +++SQ+LY+         D    
Sbjct: 232 SLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASS 291

Query: 338 GNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYIPSARECPLYLQLPDGELSKTN 397
              L +  +P  +N  E  L +S  A     VL F++Y+P     PLY+Q  DG    TN
Sbjct: 292 SYYLDMHSLPHVINPVESRLGSS--AASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATN 349

Query: 398 AFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSEELMETIEIFIGQLRQLFGLKSSYHTQ 457
           AF SP WGG++++N    ++ +     L      +++  +E+F+ QLR LFG+      Q
Sbjct: 350 AFHSPRWGGIMVYNVDSKTYNAS---VLPVRVEVDMVRVMEVFLAQLRLLFGI-----AQ 401

Query: 458 SMDGVTKFITSP--KGFAQWELDLLYRHHACSNLLSCLTTLESLSSLVQSLPRMIVMDEI 515
                   ++ P  +G   WELD L    +  NL +  TTL SL+ L+  +  +++ D++
Sbjct: 402 PQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDV 461

Query: 516 GRQPFFAPVSLHVLLAVMKELKRYMVERRKYSAFLASQ 553
             + +         +A +++    +      SAF+ASQ
Sbjct: 462 ASEVYK-------AVAAVQKSAEELASGHLASAFVASQ 492
 Score = 34.7 bits (79), Expect = 6.2
 Identities = 12/28 (42%), Positives = 19/28 (67%)

Query: 63 SFLIGLPLLLKSTEIHRSPLPSDAIAAL 90
          + ++GLPL  K+TE +R+ LP   I+ L
Sbjct: 27 AIVLGLPLWWKTTETYRASLPYSQISGL 54
>dbj|BAC11615.1| unnamed protein product [Homo sapiens]
          Length = 555

 Score =  445 bits (1133), Expect = e-123
 Identities = 92/338 (27%), Positives = 159/338 (46%), Gaps = 25/338 (7%)

Query: 221 HRHAW-VVGK--VDEAEVVSIIGKVFVKYFMNGGVEEGEASTVKREFMPVGSDGNIVLSF 277
           HR A+ ++G+  V  A+ +S+   V      +   E+  ++  +R   P+ S     ++F
Sbjct: 175 HREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRR---PLKSSLGYEITF 231

Query: 278 SLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQVLYHTPKSSYSYSDDKLG 337
           SLLN DP      W+ E   +R + P + AL    N +++SQ+LY+         D    
Sbjct: 232 SLLNPDPKSHDVYWDIEGAVRRYVQPSLNALGAAGNFSVDSQILYYAMLGVNPRFDSASS 291

Query: 338 GNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYIPSARECPLYLQLPDGELSKTN 397
              L +  +P  +N  E  L +S  A     VL F++Y+P     PLY+Q  DG    TN
Sbjct: 292 SYYLDMHSLPHVINPVESRLGSS--AASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATN 349

Query: 398 AFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSEELMETIEIFIGQLRQLFGLKSSYHTQ 457
           AF SP WGG++++N    ++ +     L      +++  +E+F+ QLR LFG+      Q
Sbjct: 350 AFHSPRWGGIMVYNVDSKTYNAS---VLPVRVEVDMVRVMEVFLAQLRLLFGI-----AQ 401

Query: 458 SMDGVTKFITSP--KGFAQWELDLLYRHHACSNLLSCLTTLESLSSLVQSLPRMIVMDEI 515
                   ++ P  +G   WELD L    +  NL +  TTL SL+ L+  +  +++ D++
Sbjct: 402 PQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDV 461

Query: 516 GRQPFFAPVSLHVLLAVMKELKRYMVERRKYSAFLASQ 553
             + +         +A +++    +      SAF+ASQ
Sbjct: 462 ASEVYK-------AVAAVQKSAEELASGHLASAFVASQ 492
 Score = 34.7 bits (79), Expect = 6.3
 Identities = 12/28 (42%), Positives = 19/28 (67%)

Query: 63 SFLIGLPLLLKSTEIHRSPLPSDAIAAL 90
          + ++GLPL  K+TE +R+ LP   I+ L
Sbjct: 27 AIVLGLPLWWKTTETYRASLPYSQISGL 54
>ref|XP_203412.1| similar to phosphatidylinositol glycan class S [Homo sapiens] [Mus
           musculus]
          Length = 555

 Score =  440 bits (1121), Expect = e-122
 Identities = 94/338 (27%), Positives = 155/338 (45%), Gaps = 25/338 (7%)

Query: 221 HRHAW-VVGK--VDEAEVVSIIGKVFVKYFMNGGVEEGEASTVKREFMPVGSDGNIVLSF 277
           HR A+ ++G+  V  A+ +S+   V      +   E+  +S  +R   P+ S     ++F
Sbjct: 175 HREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRR---PLKSSLGYEITF 231

Query: 278 SLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQVLYHTPKSSYSYSDDKLG 337
           SLLN DP      W+ E   QR + P +  L    N +++SQ+LY+         D    
Sbjct: 232 SLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASS 291

Query: 338 GNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYIPSARECPLYLQLPDGELSKTN 397
              L++  +P  +N  E  L +S  A     VL F++Y+P     PLY+Q  DG    TN
Sbjct: 292 SYSLAMHSLPHVINPVESRLGSS--AASLYPVLHFLLYVPELAHSPLYIQDKDGAPVATN 349

Query: 398 AFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSEELMETIEIFIGQLRQLFGLKSSYHTQ 457
           AF SP WGG++++N     + +     L      +++  +E+F+ QLR LFG+      Q
Sbjct: 350 AFHSPRWGGIMVYNVDPKIYNASE---LPVRVEVDMVRVMEVFLAQLRLLFGI-----AQ 401

Query: 458 SMDGVTKFITSPK--GFAQWELDLLYRHHACSNLLSCLTTLESLSSLVQSLPRMIVMDEI 515
                   ++ PK  G   WELD L    +  NL +  TTL SL+ L+  +  +++ D++
Sbjct: 402 PQVPPKCLLSGPKSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDV 461

Query: 516 GRQPFFAPVSLHVLLAVMKELKRYMVERRKYSAFLASQ 553
             + + A  ++             +      SAF ASQ
Sbjct: 462 ASEVYRAVAAVQKAAK-------ALALGHLSSAFAASQ 492
 Score = 41.7 bits (97), Expect = 0.044
 Identities = 18/44 (40%), Positives = 26/44 (58%), Gaps = 3/44 (6%)

Query: 51 RKRLVLTASVLLSFLIGLPLLLKSTEIHRSPLPSDAIAALSRRL 94
          R  L   A   ++ L+GLPL  K+TE +R+PLP   I+ L+  L
Sbjct: 18 RSALFFAA---VAILLGLPLWWKTTETYRAPLPYSDISGLNALL 58
>ref|XP_220633.1| similar to phosphatidylinositol glycan class S [Homo sapiens]
           [Rattus norvegicus]
          Length = 496

 Score =  432 bits (1099), Expect = e-119
 Identities = 89/348 (25%), Positives = 150/348 (42%), Gaps = 35/348 (10%)

Query: 221 HRHAW-VVGK--VDEAEVVSIIGKVFVKYFMNGGVEEGEASTVKREFMPVGSDGNIVLSF 277
           HR A+ ++G+  +  A+ +S+   V      +   E+  +S  +R   P+ S     ++F
Sbjct: 106 HREAFNIIGRRIIHVAQAMSLTEDVLAAALADHLPEDKWSSDKRR---PLKSSLGYEITF 162

Query: 278 SLLNADPSDWVYDWEFENIGQRMLTPVIE----------ALRPIANINIESQVLYHTPKS 327
           SLLN DP      W+       +    +E           L  I       Q+LY+    
Sbjct: 163 SLLNPDPKSHDVHWDIGGGCPTLCAAFLEPPQRCRQFLCGLSAICRAVSCEQILYYAMLG 222

Query: 328 SYSYSDDKLGGNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYIPSARECPLYLQ 387
                D       L++  +P  +N  E  L +S  A     VL F++Y+P     PLY+Q
Sbjct: 223 VNPRFDPASSSYSLAMHSLPHVINPVESRLGSS--AASLYPVLNFLLYVPELAHSPLYIQ 280

Query: 388 LPDGELSKTNAFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSEELMETIEIFIGQLRQL 447
             DG    TNAF SP WGG++++N     + +     L      ++ + +E+F+ QLR L
Sbjct: 281 DKDGAPVATNAFHSPRWGGIMVYNVDPKIYNASE---LPVRVEVDMAQVMEVFLAQLRLL 337

Query: 448 FGLKSSYHTQSMDGVTKFITSPK--GFAQWELDLLYRHHACSNLLSCLTTLESLSSLVQS 505
           FG+      Q        ++ PK  G   WELD L    +  NL +  TTL SL+ L+  
Sbjct: 338 FGI-----AQPQVPPKCLLSGPKSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGK 392

Query: 506 LPRMIVMDEIGRQPFFAPVSLHVLLAVMKELKRYMVERRKYSAFLASQ 553
           +  +++ D++  + +         +A +++    +      SAF ASQ
Sbjct: 393 ISNIVIKDDVASEVYR-------AVAAVQKAAEALALGHLSSAFAASQ 433
>ref|NP_733060.1| CG31120-PB [Drosophila melanogaster]
 gb|AAL27645.1| SD10476p [Drosophila melanogaster]
 gb|AAF56366.2| CG31120-PB [Drosophila melanogaster]
          Length = 536

 Score =  359 bits (913), Expect = 1e-97
 Identities = 69/311 (22%), Positives = 122/311 (39%), Gaps = 27/311 (8%)

Query: 257 ASTVKREFMPVGSDGNIVLSFSLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINI 316
           ++  +             +  S+LN  P      W      +  + P +  +  ++N  +
Sbjct: 185 STDERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTV 244

Query: 317 ESQVLYHTPKS---SYSYSDDKLGG-NVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQF 372
            SQ  Y               KLG    L    +P  + S   +L  S   T     +  
Sbjct: 245 RSQWKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSAS---TTDKPAINL 301

Query: 373 VVYIPSARECPLYLQL-PDGELSKT--NAFISPMWGGVVIWNPPGCSFGSKPHGALDKMS 429
           VVYIP     PL++    D  L++   +AFISP WGG +I NPP        H  L  MS
Sbjct: 302 VVYIPPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPP-------EHVCLAAMS 354

Query: 430 SEELM-------ETIEIFIGQLRQLFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLLYR 482
            +E +       + +++ + QL +L  + S    + +  V      P+   +WE +   R
Sbjct: 355 DQEAVPYHVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPR---RWEYEAYLR 411

Query: 483 HHACSNLLSCLTTLESLSSLVQSLPRMIVMDEIGRQPFFAPVSLHVLLAVMKELKRYMVE 542
             A  ++ +  +TL+SL  L+  +  +++ DE+G     +   +    A + E +     
Sbjct: 412 RSAIRHISTASSTLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADAS 471

Query: 543 RRKYSAFLASQ 553
                AF+AS+
Sbjct: 472 VLAKRAFVASE 482
>gb|EAA07616.1| agCP1277 [Anopheles gambiae str. PEST]
          Length = 590

 Score =  349 bits (888), Expect = 8e-95
 Identities = 64/309 (20%), Positives = 120/309 (38%), Gaps = 28/309 (9%)

Query: 261 KREFMPVGSDGNIVLSFSLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQV 320
           +    P+ +     +  ++LN  P      W      +  + P +  L  I N  I++Q 
Sbjct: 235 RLNSAPLQAQ--YEVLITVLNPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQW 292

Query: 321 LYHTPKS------SYSYSDDKLGG-NVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFV 373
           +Y                D KLG    L+   +P  + S E  L T I     +  +  V
Sbjct: 293 IYQVGLEGAGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKLGTQI---TDNPCIHLV 349

Query: 374 VYIPSARECPLYLQLPDG---ELSKTN--AFISPMWGGVVIWNPPGCS---FGSKPHGAL 425
           VY+P   + PL +   DG       +N  AF S  WGG+V  NP   +   +      + 
Sbjct: 350 VYVPPCAQAPLRIYRKDGQRAAPLASNVEAFTSAKWGGIVFANPAEATCVRYMETEQFSD 409

Query: 426 DKMSSEELMETIEIFIGQLRQLFGL-KSSYHTQSMDGVTKFITSPKGFAQWELDLLYRHH 484
             + +++    + + + QLR++F L  ++    +       I  P+    WE+D   R +
Sbjct: 410 VYIHAQD---VMPVLLYQLRKIFDLENNTPLLDATLVPYSTI-EPRA---WEVDTFMRTN 462

Query: 485 ACSNLLSCLTTLESLSSLVQSLPRMIVMDEIGRQPFFAPVSLHVLLAVMKELKRYMVERR 544
               + S  TTL+SL  L+  +  +++ DE+G     A   +      ++  +       
Sbjct: 463 TIYLVHSATTTLQSLIQLLGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAGRLQQAALL 522

Query: 545 KYSAFLASQ 553
              A+ +++
Sbjct: 523 AREAYTSAE 531
>ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe]
 sp|Q10351|PIGS_SCHPO GPI transamidase component PIG-S homolog
 pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA93813.1| hypothetical protein [Schizosaccharomyces pombe]
          Length = 554

 Score =  349 bits (886), Expect = 1e-94
 Identities = 75/302 (24%), Positives = 119/302 (38%), Gaps = 31/302 (10%)

Query: 252 VEEGEASTVKREFMPVGSDGNIVLSFSLLNADPSDWVYDWEFENIGQRMLTPVIEALRPI 311
           VE   A T +     V       +  SLL  + +    +W+ EN  Q+   P+IE L  +
Sbjct: 223 VEPSRAETKR----TVQFSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASL 278

Query: 312 ANINIESQVLYHTPKSSYSYSDDKLGGNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQ 371
           A +NIE+Q+ Y    +     DDK         D+P  VN+ E +L  S S   R   + 
Sbjct: 279 AKLNIETQIQYFVEDAEAYIKDDKF---CTKHADLPNLVNNFEKYL--SFSPHIREPTIH 333

Query: 372 FVVYIPSARECPLYLQLPDGELSKTNAFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSE 431
           FV+Y+PS +  PL+L+  D  +  TN+ + P WG +   N     F       L      
Sbjct: 334 FVLYVPSPQIQPLWLENEDSNIIPTNSMLLPQWGSITTIN-----FNVTEKKLL---HDV 385

Query: 432 ELMETIEIFIGQLRQLFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLLYRHHACSNLLS 491
           +L +   +    L  L G+         D     +++         D L R     + + 
Sbjct: 386 DLKDYFRVISRDLLLLLGIN--------DVPVSSLSATLA------DRLLRQRIAESCIE 431

Query: 492 CLTTLESLSSLVQSLPRMIVMDEIGRQPFFAPVSLHVLLAVMKELKRYMVERRKYSAFLA 551
              TL++L+ LV S+  M V  EI        +SL +    + +           +AF  
Sbjct: 432 ASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLNEALSYSNNAFSK 491

Query: 552 SQ 553
           SQ
Sbjct: 492 SQ 493
>ref|XP_142436.2| similar to phosphatidylinositol glycan class S [Homo sapiens] [Mus
           musculus]
          Length = 207

 Score =  302 bits (766), Expect = 2e-80
 Identities = 59/191 (30%), Positives = 92/191 (47%), Gaps = 8/191 (4%)

Query: 221 HRHAW-VVGK--VDEAEVVSIIGKVFVKYFMNGGVEEGEASTVKREFMPVGSDGNIVLSF 277
           HR A+ ++G+  V  A+ +S+   V      +   E+  +S  +R   P+ S     ++F
Sbjct: 18  HREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRR---PLKSSLGYEITF 74

Query: 278 SLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQVLYHTPKSSYSYSDDKLG 337
           SLLN DP      W+ E   QR + P +  L    N +++SQ+LY+         D    
Sbjct: 75  SLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVNPRFDPASS 134

Query: 338 GNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYIPSARECPLYLQLPDGELSKTN 397
              L++  +P  +N  E  L +S  A     VL F++Y+P     PLY+Q  DG    TN
Sbjct: 135 SYSLAMHSLPHVINPVESRLGSS--AASLYPVLHFLLYVPELAHSPLYIQDKDGAPVATN 192

Query: 398 AFISPMWGGVV 408
           AF SP WGG++
Sbjct: 193 AFHSPRWGGIM 203
>ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Saccharomyces
           cerevisiae]
 sp|Q04080|GP17_YEAST GPI transamidase component GPI17
 pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
 gb|AAB64866.1| Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
          Length = 534

 Score =  262 bits (664), Expect = 1e-68
 Identities = 66/266 (24%), Positives = 107/266 (39%), Gaps = 40/266 (15%)

Query: 267 VGSDGNIVLSFSLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQVLYHTPK 326
           +  D NI LS +LL+ D +     WE E       +P  + L P+ N  ++S ++YH   
Sbjct: 184 ISYDPNIHLSVTLLSGDGNP--VAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIVYHNDL 241

Query: 327 SSYSYSDDKLGGNVLSVG--DIPFFVNSNEWHLDTSISATGRSKVLQFVVYIPSARECPL 384
           + +S     L G+  SV   D+   ++ +E    +S++       L   +  PSA   P 
Sbjct: 242 NLHS-----LNGSCTSVTWFDLSHTIDLSEL---SSMAYYPEDSALNLAIVFPSASSSPD 293

Query: 385 YLQLPDG-----ELSKT--NAFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSEELMETI 437
            L   +G     E++    N+++ P WG ++I   P      KP+  + +   E +M   
Sbjct: 294 GLAFINGTRISDEITTLDWNSYLVPQWGVIIINKMP-----LKPNSVISEDYLEPMMYR- 347

Query: 438 EIFIGQLRQLFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLLYRHHACSNLLSCLTTLE 497
             F   + QL GL       S D ++ +IT         +D   R     NL     TL 
Sbjct: 348 --FATDIFQLLGLTEG----SQDLLSPYIT---------IDSFKRLTILQNLDKATETLW 392

Query: 498 SLSSLVQSLPRMIVMDEIGRQPFFAP 523
           SL  L Q    M +  E+      A 
Sbjct: 393 SLVKLTQQFQGMSIPREVSDNVIEAL 418
>dbj|BAB70938.1| unnamed protein product [Homo sapiens]
          Length = 184

 Score =  141 bits (353), Expect = 4e-32
 Identities = 32/130 (24%), Positives = 60/130 (45%), Gaps = 14/130 (10%)

Query: 426 DKMSSEELMETIEIFIGQLRQLFGLKSSYHTQSMDGVTKFITSP--KGFAQWELDLLYRH 483
                 +++  +E+F+ QLR LFG+      Q        ++ P  +G   WELD L   
Sbjct: 4   PVRVEVDMVRVMEVFLAQLRLLFGI-----AQPQLPPKCLLSGPTSEGLMTWELDRLLWA 58

Query: 484 HACSNLLSCLTTLESLSSLVQSLPRMIVMDEIGRQPFFAPVSLHVLLAVMKELKRYMVER 543
            +  NL +  TTL SL+ L+  +  +++ D++  + +         +A +++    +   
Sbjct: 59  RSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYK-------AVAAVQKSAEELASG 111

Query: 544 RKYSAFLASQ 553
              SAF+ASQ
Sbjct: 112 HLASAFVASQ 121
>ref|XP_194600.1| similar to phosphatidylinositol glycan class S [Homo sapiens] [Mus
           musculus]
          Length = 137

 Score = 71.3 bits (173), Expect = 6e-11
 Identities = 21/81 (25%), Positives = 36/81 (43%), Gaps = 7/81 (8%)

Query: 473 AQWELDLLYRHHACSNLLSCLTTLESLSSLVQSLPRMIVMDEIGRQPFFAPVSLHVLLAV 532
             WELD L    +  NL +  TTL SL+ L+  +  +++ D++  + + A  ++      
Sbjct: 1   MTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAK- 59

Query: 533 MKELKRYMVERRKYSAFLASQ 553
                  +      SAF ASQ
Sbjct: 60  ------ALALGHLSSAFAASQ 74
>ref|NP_501852.1| Putative endoplasmic reticulum membrane protein, with at least 2
           transmembrane domains, a coiled coil-4 domain, of
           bilaterial origin [Caenorhabditis elegans]
 pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
 emb|CAA93093.1| Hypothetical protein T14G10.7 [Caenorhabditis elegans]
          Length = 544

 Score = 41.3 bits (96), Expect = 0.056
 Identities = 42/295 (14%), Positives = 91/295 (30%), Gaps = 40/295 (13%)

Query: 257 ASTVKREFMPVGSDGNIVLSFSLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINI 316
           +   +  +       N  +    L+ +        + +    R L    E ++ I N+ +
Sbjct: 238 SHQKRLVWDSAPLATNYHIQVIHLHENSEITPESLKEQEKTARALRKFAEKVKEITNVEV 297

Query: 317 ESQVLYHTPKSSYSYSDDKLGGNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYI 376
             + L+    +      D      L+   +   V   +  L TS+       VL+FVV  
Sbjct: 298 SFEHLWDFEPTRGFLIKDVQERWALTHSAMEELVKKVDSQLQTSLQHGA---VLRFVVL- 353

Query: 377 PSARECPLYLQLPDGELSKTNAFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSEELMET 436
                 P+ +    GE   ++      WG V+  N       +    A+  +  +  M+ 
Sbjct: 354 --ETVEPVIVLDDTGE--DSHGVAVASWGAVLPRN------EATESRAIAAIRIQMGMDA 403

Query: 437 IEIFIGQLRQLFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLLYRHHACSNLLSCLTTL 496
                        L   +H   +              QWE+       +  N +   + +
Sbjct: 404 ------------ELSHGWHRPPV-----------AICQWEVARARLRASVDNAMRAASAV 440

Query: 497 ESLSSLVQSLPRMIVMDEIGRQPFFA---PVSLHVLLAVMKELKRYMVERRKYSA 548
            +L  L Q +  +++ D++  +   A      +     ++   +     R   SA
Sbjct: 441 RALDQLAQKISNIVINDDVADRATRAVEILNDVVRPGRMLDFERLLEARRLADSA 495
CPU time:   152.87 user secs.	    3.71 sys. secs	  156.58 total secs.

  Database: nr
    Posted date:  Jan 18, 2003  1:23 PM
  Number of letters in database: 421,297,171
  Number of sequences in database:  1,315,992
  
Lambda     K      H
   0.310    0.157    0.439 

Gapped
Lambda     K      H
   0.270   0.0549    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 409197252
Number of Sequences: 1315992
Number of extensions: 17058420
Number of successful extensions: 79943
Number of sequences better than 10.0: 88
Number of HSP's better than 10.0 without gapping: 36
Number of HSP's successfully gapped in prelim test: 54
Number of HSP's that attempted gapping in prelim test: 79647
Number of HSP's gapped (non-prelim): 207
length of query: 557
length of database: 421,297,171
effective HSP length: 60
effective length of query: 497
effective length of database: 342,337,651
effective search space: 170141812547
effective search space used: 170141812547
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.0 bits)
S2: 77 (34.2 bits)