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GPI Anchor Biosynthesis Report: AN5150.1 (PIG-S family, Aspergillus nidulans)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (551 letters)

Database: nr
           1,543,518 sequences; 504,497,942 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 28 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

emb|CAD60556.1| unnamed protein product [Podospora anserina] 657 0.0
ref|XP_220633.2| similar to phosphatidylinositol glycan class S ... 655 0.0
gb|AAQ89470.1| PIGS [Homo sapiens] 655 0.0
ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapie... 652 0.0
dbj|BAC11615.1| unnamed protein product [Homo sapiens] 644 0.0
ref|XP_325888.1| hypothetical protein [Neurospora crassa] >gi|28... 644 0.0
ref|XP_203412.1| similar to phosphatidylinositol glycan class S ... 638 0.0
gb|EAA57290.1| hypothetical protein MG08259.4 [Magnaporthe grise... 609 e-173
gb|AAO39601.1| GM10512p [Drosophila melanogaster] 548 e-154
ref|NP_733060.1| CG31120-PB [Drosophila melanogaster] >gi|169020... 547 e-154
ref|XP_311969.1| ENSANGP00000010967 [Anopheles gambiae] >gi|2129... 535 e-150
ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Sacc... 535 e-150
ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe... 458 e-127
ref|NP_187374.2| expressed protein [Arabidopsis thaliana] >gi|17... 391 e-107
gb|AAF20228.1| unknown protein [Arabidopsis thaliana] 352 2e-95
gb|AAH01319.2| PIGS protein [Homo sapiens] 287 5e-76
dbj|BAB92563.1| P0497A05.6 [Oryza sativa (japonica cultivar-group)] 274 3e-72
ref|XP_142436.3| similar to phosphatidylinositol glycan class S ... 259 1e-67
dbj|BAB70938.1| unnamed protein product [Homo sapiens] 207 5e-52
emb|CAE45912.1| hypothetical protein [Homo sapiens] 195 2e-48
ref|NP_501852.1| phosphatidylinositol glycan class S like (4K912... 132 2e-29
Sequences with E-value WORSE than threshold

ref|NP_701826.1| hypothetical protein [Plasmodium falciparum 3D7... 42 0.035
Alignments
>emb|CAD60556.1| unnamed protein product [Podospora anserina]
          Length = 542

 Score =  657 bits (1677), Expect = 0.0
 Identities = 279/534 (52%), Positives = 369/534 (68%), Gaps = 36/534 (6%)

Query: 3   SKLSASTLPPPEKPSAIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAG 62
           +  S+  LPPPEKPS ++ RS V+ +FW ++L LG PIWWKTT+IYRA+LP+++ML+WA 
Sbjct: 24  APTSSKKLPPPEKPSDVRRRSHVILSFWLIVLLLGLPIWWKTTTIYRADLPLEDMLEWAD 83

Query: 63  GKSCRPVFPLEIRFETPSLPDVEAQQLLRITQHTLDDLNEFTAHHLRLKLAEDEAEQTTQ 122
           GK+CRPVFPL I  +  SL D EAQ LLR+TQH LDDLN+F  HHLRL++ ++  +Q + 
Sbjct: 84  GKACRPVFPLRISIQATSLQDQEAQNLLRLTQHALDDLNDFAGHHLRLQMGDE--KQDSA 141

Query: 123 KADTALTVRLQAQGDLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAAELQHL 182
              +ALT+RL       G  + L     +LDI YP + IP P++ +S LA++IA++L+  
Sbjct: 142 DNGSALTIRLTPG---EGTTATLDPYEPILDITYPQNTIPSPTASSSALATYIASQLRTT 198

Query: 183 FSEEKAIMSHILSNNAIGNYAVSTPNSLAHQISPQLAESITKRLRRSMKYAETYHLAFSL 242
           F+EE+A +S++LS N++     S    L   +SP+ AE++ KR  RS+KYA TYHL FSL
Sbjct: 199 FAEEQATISYLLSTNSMP----SEHRPL--GLSPETAEALAKRTTRSLKYAPTYHLTFSL 252

Query: 243 FTPGPAPSSWDIESAVQGYISPIVQALSPISNFTVDTQVQLYATFSPTTPVPEYDETQGV 302
           FT GP PSSWDIE+A++ Y+ P++  LSPI NFT+DTQVQLYA+      V         
Sbjct: 253 FTSGPLPSSWDIETAIEEYMKPVLDVLSPIHNFTIDTQVQLYASPGVQNQV--------- 303

Query: 303 WTLKEENLSAFINAAEWPLNPSIGSGPTINFILYVPAPSQ-SPLLVQESKATSWIIPQWG 361
             L +E+LS+FINAAEWPL+PSIG  PTINFI++V   +  SP       + SW+IPQWG
Sbjct: 304 --LNKEDLSSFINAAEWPLSPSIGGAPTINFIVFVGNQTIASPSQEAAPTSHSWLIPQWG 361

Query: 362 GVFILNPPLSTTDGSAQSNPPHLPRDALRAPFLTFSHQLLSLLGTPSTPPSLPFRLQTLI 421
            VF+L+ P  TT         H+    L+ P LTF+  LLSL GTP +  SLP RL TL 
Sbjct: 362 TVFLLSLPGDTT---------HVAATTLKQPLLTFTSHLLSLTGTPES-GSLPLRLSTLA 411

Query: 422 RIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSHLSSSCESLQNGQFQAA 481
           RIR+A +LL ASST+GSLARL  +LPSI IP++VA  V  T+SHL  +C++L        
Sbjct: 412 RIRSADLLLRASSTLGSLARLAMALPSISIPSSVADGVTKTMSHLRLACDNLGGT---EG 468

Query: 482 LASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVPLVLGLLKEV 535
           LA AR+AE EAER+FFEKSMVGQ+YFPDEHK+AVYLPLLGP+ VPLV+GL+ E+
Sbjct: 469 LAHARIAEVEAERAFFEKSMVGQLYFPDEHKIAVYLPLLGPVAVPLVMGLINEI 522
>ref|XP_220633.2| similar to phosphatidylinositol glycan class S [Rattus norvegicus]
          Length = 564

 Score =  655 bits (1674), Expect = 0.0
 Identities = 153/554 (27%), Positives = 245/554 (43%), Gaps = 42/554 (7%)

Query: 18  AIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAGGKSCRPVFPLEIRFE 77
            +    +    F AV + LG P+WWKTT  YRA LP  E+         R + P+ + F 
Sbjct: 21  EVVRGKRAALFFAAVAILLGLPLWWKTTETYRAPLPYSEI-SGLNALQLRLMVPVTVVFT 79

Query: 78  TPSLPDVEAQQLLRITQHTLD-DLNEFTAHHLRLKLAEDEAEQTTQKADTALTVRLQAQG 136
             S+P  + ++L     H  +  L        R + A   A +  ++A +  +V  +A+ 
Sbjct: 80  RDSVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEEALSLGSVH-EAEA 138

Query: 137 DLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAAELQHLFSEEKAIMSHILSN 196
            L  P  +     T+  I    S +P        + S+I  E   +       M H  + 
Sbjct: 139 LLAEPEKQAEGSLTVYVISEHSSLLP------QDMMSYIGPERTAIVRG----MIHREAF 188

Query: 197 NAIGNYAVS-------TPNSLAHQISPQLAE-SITKRLRRSMKYAETYHLAFSLFTPGPA 248
           N IG   +        T + LA  ++  L E   +   RR +K +  Y + FSL  P P 
Sbjct: 189 NIIGRRIIHVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLNPDPK 248

Query: 249 PSS--WDIESAVQGYISPIVQALSPISNFTVDTQVQLYATFSPTTPVPEYDETQGVWTLK 306
                WDIE  VQ Y+ P +  LS   NF+VD+Q+  YA        P +D     ++L 
Sbjct: 249 SHDVHWDIEEGVQRYVQPFLNRLSAAGNFSVDSQILYYAMLGVN---PRFDPASSSYSLA 305

Query: 307 EENLSAFINAAEWPLNPSIGS-GPTINFILYVPAPSQSPLLVQESKA-----TSWIIPQW 360
             +L   IN  E  L  S  S  P +NF+LYVP  + SPL +Q+         ++  P+W
Sbjct: 306 MHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRW 365

Query: 361 GGVFILNPPLSTTDGSAQSNPPHLPRDALRAPFLTFSHQLLS----------LLGTPSTP 410
           GG+ + N      + S       +    +   FL     L            LL  P + 
Sbjct: 366 GGIMVYNVDPKIYNASELPVRVEVDMAQVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSE 425

Query: 411 PSLPFRLQTLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSHLSSSC 470
             + + L  L+  R+   L +A++T+ SLA+L   + +I I   VAS V   ++ +  + 
Sbjct: 426 GLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAA 485

Query: 471 ESLQNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVPLVLG 530
           E+L  G   +A A+++ A   +ER+FF+ S++  +YFPD+ K A+Y+PL  P+ VP++L 
Sbjct: 486 EALALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLS 545

Query: 531 LLKEVKRVVTGWKA 544
           L+K        WK 
Sbjct: 546 LVKIFLETHKSWKK 559
>gb|AAQ89470.1| PIGS [Homo sapiens]
          Length = 547

 Score =  655 bits (1673), Expect = 0.0
 Identities = 155/559 (27%), Positives = 252/559 (44%), Gaps = 48/559 (8%)

Query: 16  PSAIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAGGKSCRPVFPLEIR 75
           PS +    +    F AV + LG P+WWKTT  YRA LP  ++         R + P+ + 
Sbjct: 2   PSEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQI-SGLNALQLRLMVPVTVV 60

Query: 76  FETPSLPDVEAQQLLRITQHTLD-DLNEFTAHHLRLKLAEDEAEQTTQKADTALTVRLQA 134
           F   S+P  + ++L     H  +  L        R + A   A    ++A ++ +V+ +A
Sbjct: 61  FTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQ-EA 119

Query: 135 QGDLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAAELQHLFSEEKAIMSHIL 194
           +  L  P+ +     T+  I    S +P        + S+I  +   +       + H  
Sbjct: 120 EAMLDEPQEQAEGSLTVYVISEHSSLLP------QDMMSYIGPKRTAVVRG----IMHRE 169

Query: 195 SNNAIGNYAVS-------TPNSLAHQISPQLAE-SITKRLRRSMKYAETYHLAFSLFTPG 246
           + N IG   V        T + LA  ++  L E   +   RR +K +  Y + FSL  P 
Sbjct: 170 AFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPD 229

Query: 247 PAPSS--WDIESAVQGYISPIVQALSPISNFTVDTQVQLYATFSPTTPVPEYDETQGVWT 304
           P      WDIE AV+ Y+ P + AL    NF+VD+Q+  YA        P +D     + 
Sbjct: 230 PKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVN---PRFDSASSSYY 286

Query: 305 LKEENLSAFINAAEWPLNPSIGS-GPTINFILYVPAPSQSPLLVQESKA-----TSWIIP 358
           L   +L   IN  E  L  S  S  P +NF+LYVP  + SPL +Q+         ++  P
Sbjct: 287 LDMHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSP 346

Query: 359 QWGGVFILNPPLSTTDGSAQSNPPHLPRDALRAPFLTFSHQLLSLLGT------------ 406
           +WGG+ + N    T + S    P  +  D +R     F  QL  L G             
Sbjct: 347 RWGGIMVYNVDSKTYNASVL--PVRVEVDMVR-VMEVFLAQLRLLFGIAQPQLPPKCLLS 403

Query: 407 -PSTPPSLPFRLQTLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSH 465
            P++   + + L  L+  R+   L +A++T+ SLA+L   + +I I   VAS V   ++ 
Sbjct: 404 GPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAA 463

Query: 466 LSSSCESLQNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGV 525
           +  S E L +G   +A  +++ A   +E +FF+ S++  +YFPD+ K A+Y+PL  P+ V
Sbjct: 464 VQKSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAV 523

Query: 526 PLVLGLLKEVKRVVTGWKA 544
           P++L L+K        W+ 
Sbjct: 524 PILLSLVKIFLETRKSWRK 542
>ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapiens]
 sp|Q96S52|PIGS_HUMAN GPI transamidase component PIG-S (Phosphatidylinositol-glycan
           biosynthesis, class S protein)
 dbj|BAB60853.1| phosphatidyl inositol glycan class S [Homo sapiens]
          Length = 555

 Score =  652 bits (1664), Expect = 0.0
 Identities = 153/557 (27%), Positives = 250/557 (44%), Gaps = 48/557 (8%)

Query: 18  AIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAGGKSCRPVFPLEIRFE 77
            +    +    F AV + LG P+WWKTT  YRA LP  ++         R + P+ + F 
Sbjct: 12  EVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQI-SGLNALQLRLMVPVTVVFT 70

Query: 78  TPSLPDVEAQQLLRITQHTLD-DLNEFTAHHLRLKLAEDEAEQTTQKADTALTVRLQAQG 136
             S+P  + ++L     H  +  L        R + A   A    ++A ++ +V+ +A+ 
Sbjct: 71  RESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQ-EAEA 129

Query: 137 DLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAAELQHLFSEEKAIMSHILSN 196
            L  P+ +     T+  I    S +P        + S+I  +   +       + H  + 
Sbjct: 130 MLDEPQEQAEGSLTVYVISEHSSLLP------QDMMSYIGPKRTAVVRG----IMHREAF 179

Query: 197 NAIGNYAVS-------TPNSLAHQISPQLAE-SITKRLRRSMKYAETYHLAFSLFTPGPA 248
           N IG   V        T + LA  ++  L E   +   RR +K +  Y + FSL  P P 
Sbjct: 180 NIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPK 239

Query: 249 PSS--WDIESAVQGYISPIVQALSPISNFTVDTQVQLYATFSPTTPVPEYDETQGVWTLK 306
                WDIE AV+ Y+ P + AL    NF+VD+Q+  YA        P +D     + L 
Sbjct: 240 SHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVN---PRFDSASSSYYLD 296

Query: 307 EENLSAFINAAEWPLNPSIGS-GPTINFILYVPAPSQSPLLVQESKA-----TSWIIPQW 360
             +L   IN  E  L  S  S  P +NF+LYVP  + SPL +Q+         ++  P+W
Sbjct: 297 MHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRW 356

Query: 361 GGVFILNPPLSTTDGSAQSNPPHLPRDALRAPFLTFSHQLLSLLGT-------------P 407
           GG+ + N    T + S    P  +  D +R     F  QL  L G              P
Sbjct: 357 GGIMVYNVDSKTYNASVL--PVRVEVDMVR-VMEVFLAQLRLLFGIAQPQLPPKCLLSGP 413

Query: 408 STPPSLPFRLQTLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSHLS 467
           ++   + + L  L+  R+   L +A++T+ SLA+L   + +I I   VAS V   ++ + 
Sbjct: 414 TSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQ 473

Query: 468 SSCESLQNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVPL 527
            S E L +G   +A  +++ A   +E +FF+ S++  +YFPD+ K A+Y+PL  P+ VP+
Sbjct: 474 KSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPI 533

Query: 528 VLGLLKEVKRVVTGWKA 544
           +L L+K        W+ 
Sbjct: 534 LLSLVKIFLETRKSWRK 550
>dbj|BAC11615.1| unnamed protein product [Homo sapiens]
          Length = 555

 Score =  644 bits (1646), Expect = 0.0
 Identities = 153/557 (27%), Positives = 249/557 (44%), Gaps = 48/557 (8%)

Query: 18  AIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAGGKSCRPVFPLEIRFE 77
            +    +    F AV + LG P+WWKTT  YRA LP  ++         R + P+ + F 
Sbjct: 12  EVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQI-SGLNALQLRLMVPVTVVFT 70

Query: 78  TPSLPDVEAQQLLRITQHTLD-DLNEFTAHHLRLKLAEDEAEQTTQKADTALTVRLQAQG 136
             S+P  + ++L     H  +  L        R + A   A    ++A ++ +V+ +A+ 
Sbjct: 71  RESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQ-EAEA 129

Query: 137 DLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAAELQHLFSEEKAIMSHILSN 196
            L  P+ +     T+  I    S +P        + S+I  +   +       + H  + 
Sbjct: 130 MLDEPQEQAEGSLTVYVISEHSSLLP------QDMMSYIGPKRTAVVRG----IMHREAF 179

Query: 197 NAIGNYAVS-------TPNSLAHQISPQLAE-SITKRLRRSMKYAETYHLAFSLFTPGPA 248
           N IG   V        T + LA  ++  L E   +   RR +K +  Y + FSL  P P 
Sbjct: 180 NIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPK 239

Query: 249 PSS--WDIESAVQGYISPIVQALSPISNFTVDTQVQLYATFSPTTPVPEYDETQGVWTLK 306
                WDIE AV+ Y+ P + AL    NF+VD+Q+  YA        P +D     + L 
Sbjct: 240 SHDVYWDIEGAVRRYVQPSLNALGAAGNFSVDSQILYYAMLGVN---PRFDSASSSYYLD 296

Query: 307 EENLSAFINAAEWPLNPSIGS-GPTINFILYVPAPSQSPLLVQESKA-----TSWIIPQW 360
             +L   IN  E  L  S  S  P +NF+LYVP  + SPL +Q+         ++  P+W
Sbjct: 297 MHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRW 356

Query: 361 GGVFILNPPLSTTDGSAQSNPPHLPRDALRAPFLTFSHQLLSLLGT-------------P 407
           GG+ + N    T + S    P  +  D +R     F  QL  L G              P
Sbjct: 357 GGIMVYNVDSKTYNASVL--PVRVEVDMVR-VMEVFLAQLRLLFGIAQPQLPPKCLLSGP 413

Query: 408 STPPSLPFRLQTLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSHLS 467
           ++   + + L  L+  R+   L +A++T+ SLA+L   + +I I   VAS V   ++ + 
Sbjct: 414 TSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQ 473

Query: 468 SSCESLQNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVPL 527
            S E L +G   +A  +++ A   +E +FF+ S++  +YFPD  K A+Y+PL  P+ VP+
Sbjct: 474 KSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDYQKFAIYIPLFLPMAVPI 533

Query: 528 VLGLLKEVKRVVTGWKA 544
           +L L+K        W+ 
Sbjct: 534 LLSLVKIFLETRKSWRK 550
>ref|XP_325888.1| hypothetical protein [Neurospora crassa]
 gb|EAA30387.1| hypothetical protein [Neurospora crassa]
          Length = 568

 Score =  644 bits (1645), Expect = 0.0
 Identities = 281/554 (50%), Positives = 374/554 (66%), Gaps = 46/554 (8%)

Query: 3   SKLSASTLPPPEKPSAIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAG 62
           ++ S    PPPEK S I+ R  V+ +FW ++L LG P+WWKTT+I+RA LP++EMLDWA 
Sbjct: 40  AQTSTRKEPPPEKLSDIRRRIWVIVSFWLIVLLLGLPVWWKTTTIHRANLPLKEMLDWAD 99

Query: 63  GKSCRPVFPLEIRFETPSLPDVEAQQLLRITQHTLDDLNEFTAHHLRLKLAEDEAEQT-T 121
           GK+CRPVFPL I  +  SLP+ EAQ LLR+TQH LDDLN+F+ HHLRL+L+   A Q   
Sbjct: 100 GKACRPVFPLRISIQANSLPEQEAQNLLRLTQHALDDLNDFSGHHLRLQLSSPAAPQKRN 159

Query: 122 QKADTALTVRLQAQGDLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAAELQH 181
            + DTALT+RL     +T   + L     +LDI YPP+  P  +S +S LA+++A +L+ 
Sbjct: 160 DEPDTALTIRLLPGDAVT---ATLDPYEPVLDITYPPNAAPSATSASSSLATYVAGQLRS 216

Query: 182 LFSEEKAIMSHILSNNAIGNYAVSTPNSLAHQISPQLAESITKRLRRSMKYAETYHLAFS 241
            F+EE+AI+S++LS+N+I        +     ++P+ AES+ KR  RS+KYA TYHL FS
Sbjct: 217 TFAEEQAIISYLLSSNSIP------TDYRPQSLTPEAAESLAKRTTRSLKYAPTYHLTFS 270

Query: 242 LFTPGPAPSSWDIESAVQGYISPIVQALSPISNFTVDTQVQLYATFSPTTPVPEYDETQG 301
           LFT GP PSSWDIE+A+Q Y+ P++  LSPI NFT+DTQVQLYA+      V        
Sbjct: 271 LFTSGPLPSSWDIEAAIQDYLKPVLGLLSPIHNFTIDTQVQLYASPGVQNQV-------- 322

Query: 302 VWTLKEENLSAFINAAEWPLNPSIGSGPTINFILYVPAPSQSPLLVQ--------ESKAT 353
              L +++LS+FINAAEWPL+PSIG  PT+NFI++V   + +              + + 
Sbjct: 323 ---LSKDDLSSFINAAEWPLSPSIGGAPTVNFIVFVGNQTIALSSENAHDSAEGGLTTSQ 379

Query: 354 SWIIPQWGGVFILNPPLSTTDGSAQSNPPHLPRDALRAPFLTFSHQLLSLLGTPSTPPSL 413
           SW+IPQWG V++L+ P  T          H+  DAL+ P LTF+  LLSL GTPS+  SL
Sbjct: 380 SWLIPQWGTVYLLSLPHDTA---------HVSVDALKQPLLTFTSHLLSLTGTPSS-GSL 429

Query: 414 PFRLQTLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSHLSSSCESL 473
           P RL TL RIR+A +LL ASST+GSLARL  +L SI IP++VA  V+ T+ HL  +CE+L
Sbjct: 430 PLRLSTLSRIRSADLLLRASSTLGSLARLALALKSISIPSSVADGVSKTIVHLRQACETL 489

Query: 474 QNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVPLVLGLLK 533
                   L  AR+AEAEAER+FFEKSMVGQ+YFPDEHKVAVYLPLLGP+ VPLV+G+L 
Sbjct: 490 GE---AEGLEHARIAEAEAERAFFEKSMVGQLYFPDEHKVAVYLPLLGPVAVPLVMGMLN 546

Query: 534 EVKRVVTGWKARRQ 547
           E+K     W+ R++
Sbjct: 547 ELK----AWRKRKR 556
>ref|XP_203412.1| similar to phosphatidylinositol glycan class S [Mus musculus]
 gb|AAH58979.1| Unknown (protein for MGC:67343) [Mus musculus]
          Length = 555

 Score =  638 bits (1629), Expect = 0.0
 Identities = 153/557 (27%), Positives = 250/557 (44%), Gaps = 48/557 (8%)

Query: 18  AIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAGGKSCRPVFPLEIRFE 77
            +    +    F AV + LG P+WWKTT  YRA LP  ++         R + P+ + F 
Sbjct: 12  EVVRGKRSALFFAAVAILLGLPLWWKTTETYRAPLPYSDI-SGLNALLLRLMVPVTVVFT 70

Query: 78  TPSLPDVEAQQLLRITQHTLD-DLNEFTAHHLRLKLAEDEAEQTTQKADTALTVRLQAQG 136
             S+P  + ++L     H  +  L        R + A   A +  ++A +  +V  +A+ 
Sbjct: 71  RDSVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEEALSLGSVH-EAEA 129

Query: 137 DLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAAELQHLFSEEKAIMSHILSN 196
            L  P  +     T+  I    S +P        + S+I  E   +       + H  + 
Sbjct: 130 MLAEPEKQAEGSLTVYVISEHSSLLP------QDMMSYIGPERTAVVRG----LIHREAF 179

Query: 197 NAIGNYAVS-------TPNSLAHQISPQLAE-SITKRLRRSMKYAETYHLAFSLFTPGPA 248
           N IG   V        T + LA  ++  L E   +   RR +K +  Y + FSL  P P 
Sbjct: 180 NIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRRPLKSSLGYEITFSLLNPDPK 239

Query: 249 PSS--WDIESAVQGYISPIVQALSPISNFTVDTQVQLYATFSPTTPVPEYDETQGVWTLK 306
                WDIE AVQ ++ P +  LS   NF+VD+Q+  YA        P +D     ++L 
Sbjct: 240 SHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAMLGVN---PRFDPASSSYSLA 296

Query: 307 EENLSAFINAAEWPLNPSIGS-GPTINFILYVPAPSQSPLLVQESKA-----TSWIIPQW 360
             +L   IN  E  L  S  S  P ++F+LYVP  + SPL +Q+         ++  P+W
Sbjct: 297 MHSLPHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRW 356

Query: 361 GGVFILNPPLSTTDGSAQSNPPHLPRDALRAPFLTFSHQLLSLLGT-------------P 407
           GG+ + N      + S    P  +  D +R     F  QL  L G              P
Sbjct: 357 GGIMVYNVDPKIYNAS--ELPVRVEVDMVR-VMEVFLAQLRLLFGIAQPQVPPKCLLSGP 413

Query: 408 STPPSLPFRLQTLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSHLS 467
            +   + + L  L+  R+   L +A++T+ SLA+L   + +I I   VAS V   ++ + 
Sbjct: 414 KSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQ 473

Query: 468 SSCESLQNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVPL 527
            + ++L  G   +A A+++ A   +ER+FF+ S++  +YFPD+ K A+Y+PL  P+ VP+
Sbjct: 474 KAAKALALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPI 533

Query: 528 VLGLLKEVKRVVTGWKA 544
           +L L+K  +     WK 
Sbjct: 534 LLSLVKIFQETRKSWKK 550
>gb|EAA57290.1| hypothetical protein MG08259.4 [Magnaporthe grisea 70-15]
          Length = 558

 Score =  609 bits (1555), Expect = e-173
 Identities = 258/549 (46%), Positives = 350/549 (62%), Gaps = 51/549 (9%)

Query: 7   ASTLPPPEKPSAIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAGGKSC 66
            +  PPPEK + ++ RS V+ +FWA+IL +G PIWW TTS+YRA LP+ EM+DWA G++C
Sbjct: 40  TNKDPPPEKATDVRRRSYVILSFWALILSVGLPIWWHTTSVYRASLPLDEMMDWADGRAC 99

Query: 67  RPVFPLEIRFETPSLPDVEAQQLLRITQHTLDDLN---EFTAHHLRLKLAEDEAEQTTQK 123
           RP FPL I  E  ++   E   L+++TQ  LD L+   + T   LRL +A++ +      
Sbjct: 100 RPTFPLTIAIEKGNMRQDETDVLIQMTQMVLDSLSESTDSTDRQLRLTVADETSVDDE-- 157

Query: 124 ADTALTVRLQAQGDLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAAELQHLF 183
              ALTVRL      T   + L+ +  +L + Y  + +P P      LAS IA ++  + 
Sbjct: 158 -KIALTVRLNPGDTTT---TALQPEQPILQVTYAANTLPSPG-----LASRIARDVHAVL 208

Query: 184 SEEKAIMSHILSNNAIGNYAVSTPNSLAHQISPQLAESITKRLRRSMKYAETYHLAFSLF 243
           SEE+A++S     N +   + ST  SL+  +SP +A ++ KR  RS+KYA TYHL FSLF
Sbjct: 209 SEEQAVVS-----NLVAEASGSTKPSLS-GVSPAVAAAVAKRSTRSLKYAPTYHLTFSLF 262

Query: 244 TPGPAPSSWDIESAVQGYISPIVQALSPISNFTVDTQVQLYATFSPTTPVPEYDETQGVW 303
           T G +PSSWDI++AV+ Y+ P++  LSPI NFT+DTQVQLYA     + V          
Sbjct: 263 TAGSSPSSWDIDAAVEQYMRPVLDVLSPIHNFTIDTQVQLYAAPGVQSDV---------- 312

Query: 304 TLKEENLSAFINAAEWPLNPSIGSGPTINFILYVPAPSQS-PLLVQESK----ATSWIIP 358
            L +++LS+FINAAEWPL+PSIG  PTINF+++V   S S P     S     A SW+IP
Sbjct: 313 -LSKDDLSSFINAAEWPLSPSIGGAPTINFVVFVGNQSISLPTTEAGSDATATAQSWLIP 371

Query: 359 QWGGVFILNPPLSTTDGSAQSNPPHLPRDALRAPFLTFSHQLLSLLGTPSTPPSLPFRLQ 418
           QWG V+++             N   +P  +LRAP  TF+  LLSLLGTP T  SLP RL 
Sbjct: 372 QWGTVYLVP--------QTAENANKVPAQSLRAPMATFTAHLLSLLGTPETSGSLPLRLA 423

Query: 419 TLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSHLSSSCESLQNGQF 478
            L RIR+A ++L ASS++GSLARL+ +LP I IP +VA  V+TT+ HL  +C++L     
Sbjct: 424 GLRRIRSADLVLRASSSLGSLARLSRALPGIAIPRSVADGVSTTIRHLRQACDALGT--- 480

Query: 479 QAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVPLVLGLLKEVKRV 538
            A LA AR+A+  A+++FFEKSMVGQ+YFPDEHKVAVYLPLLGP+GVPLV+GLL E+K  
Sbjct: 481 SAGLAHARIAQVAADKAFFEKSMVGQLYFPDEHKVAVYLPLLGPVGVPLVMGLLNELK-- 538

Query: 539 VTGWKARRQ 547
              W+ RRQ
Sbjct: 539 --AWRKRRQ 545
>gb|AAO39601.1| GM10512p [Drosophila melanogaster]
          Length = 559

 Score =  548 bits (1397), Expect = e-154
 Identities = 124/574 (21%), Positives = 219/574 (37%), Gaps = 75/574 (13%)

Query: 6   SASTLPPPEKPSAIQT-RSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAGGK 64
           S+S + P  K       R     AF  VI+ +G P+WW+TT++YR  LP  E+L  +   
Sbjct: 28  SSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSK-- 85

Query: 65  SCRPVFPLEIRFETPSLPDVEAQQLLRITQHTLDDLNEFTAHHLRLKLAEDEAEQTTQKA 124
                 P++   +        ++  L +     +  ++     +  K             
Sbjct: 86  -----IPIKTAVQVAIYTQQTSRGQL-LIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHT 139

Query: 125 DTALT-------VRLQAQGDLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAA 177
             AL        V+                   +L      + +   +  N      IA 
Sbjct: 140 PAALEKLLLENHVQSVGDFMFIEWPKL---QEELLLTTERSALMRSDTPSNK-----IAQ 191

Query: 178 ELQHLFSEEKAIMSHILSNNAIGNYAVSTPNSLAHQISPQLAESITKRLRRSMKYAETYH 237
            L             IL    I     +     A   +PQ                  Y 
Sbjct: 192 LLHA----------KILQTYRINQILSTDERMGAKSEAPQ----------------PAYD 225

Query: 238 LAFSLFTPGPA--PSSWDIESAVQGYISPIVQALSPISNFTVDTQVQL-YATFSPTTPVP 294
           +  S+  P P    + W+I  AV+ YI P +  +S +SN+TV +Q +   A  +    V 
Sbjct: 226 VIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVR 285

Query: 295 EYDETQGVWTLKEENLSAFINAAEWPLNPSIGSGPTINFILYVPAPSQSPLLVQES---- 350
           +  +    + L+E  L   + +    L+ S    P IN ++Y+P    +PL +  S    
Sbjct: 286 DQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCHIAPLHIYNSKDQR 345

Query: 351 ----KATSWIIPQWGGVFILNPPLSTTDGS---AQSNPPHLPRDALRAPFLTFSHQLLSL 403
                  ++I P WGG  I NPP      +    ++ P H+         L   H+LL +
Sbjct: 346 LTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLDI 405

Query: 404 --------LGTPSTPPSLPFRLQTLIRIRAATV--LLSASSTMGSLARLTESLPSIPIPA 453
                   +         P R +    +R + +  + +ASST+ SL +L + +  I I  
Sbjct: 406 SSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSLIKLLDQISYIVIDD 465

Query: 454 TVASSVATTLSHLSSSCESLQNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKV 513
            V +++  + + + ++  +L   +   A   A+ A   +ER FF+ S++ Q+YFPDE K 
Sbjct: 466 EVGTAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGFFDASLLAQLYFPDEQKY 525

Query: 514 AVYLPLLGPIGVPLVLGLLKEVKRVVTGWKARRQ 547
           A+Y+PL  PI VP++      ++ V+   +  +Q
Sbjct: 526 AIYIPLFLPIMVPVLSSF-NMLRGVLQARRKEKQ 558
>ref|NP_733060.1| CG31120-PB [Drosophila melanogaster]
 gb|AAL27645.1| SD10476p [Drosophila melanogaster]
 gb|AAF56366.2| CG31120-PB [Drosophila melanogaster]
          Length = 536

 Score =  547 bits (1396), Expect = e-154
 Identities = 124/574 (21%), Positives = 219/574 (37%), Gaps = 75/574 (13%)

Query: 6   SASTLPPPEKPSAIQT-RSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAGGK 64
           S+S + P  K       R     AF  VI+ +G P+WW+TT++YR  LP  E+L  +   
Sbjct: 5   SSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSK-- 62

Query: 65  SCRPVFPLEIRFETPSLPDVEAQQLLRITQHTLDDLNEFTAHHLRLKLAEDEAEQTTQKA 124
                 P++   +        ++  L +     +  ++     +  K             
Sbjct: 63  -----IPIKTAVQVAIYTQQTSRGQL-LIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHT 116

Query: 125 DTALT-------VRLQAQGDLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAA 177
             AL        V+                   +L      + +   +  N      IA 
Sbjct: 117 PAALEKLLLENHVQSVGDFMFIEWPKL---QEELLLTTERSALMRSDTPSNK-----IAQ 168

Query: 178 ELQHLFSEEKAIMSHILSNNAIGNYAVSTPNSLAHQISPQLAESITKRLRRSMKYAETYH 237
            L             IL    I     +     A   +PQ                  Y 
Sbjct: 169 LLHA----------KILQTYRINQILSTDERMGAKSEAPQ----------------PAYD 202

Query: 238 LAFSLFTPGPA--PSSWDIESAVQGYISPIVQALSPISNFTVDTQVQL-YATFSPTTPVP 294
           +  S+  P P    + W+I  AV+ YI P +  +S +SN+TV +Q +   A  +    V 
Sbjct: 203 VIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVR 262

Query: 295 EYDETQGVWTLKEENLSAFINAAEWPLNPSIGSGPTINFILYVPAPSQSPLLVQES---- 350
           +  +    + L+E  L   + +    L+ S    P IN ++Y+P    +PL +  S    
Sbjct: 263 DQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCHIAPLHIYNSKDQR 322

Query: 351 ----KATSWIIPQWGGVFILNPPLSTTDGS---AQSNPPHLPRDALRAPFLTFSHQLLSL 403
                  ++I P WGG  I NPP      +    ++ P H+         L   H+LL +
Sbjct: 323 LTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLDI 382

Query: 404 --------LGTPSTPPSLPFRLQTLIRIRAATV--LLSASSTMGSLARLTESLPSIPIPA 453
                   +         P R +    +R + +  + +ASST+ SL +L + +  I I  
Sbjct: 383 SSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSLIKLLDQISYIVIDD 442

Query: 454 TVASSVATTLSHLSSSCESLQNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKV 513
            V +++  + + + ++  +L   +   A   A+ A   +ER FF+ S++ Q+YFPDE K 
Sbjct: 443 EVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGFFDASLLAQLYFPDEQKY 502

Query: 514 AVYLPLLGPIGVPLVLGLLKEVKRVVTGWKARRQ 547
           A+Y+PL  PI VP++      ++ V+   +  +Q
Sbjct: 503 AIYIPLFLPIMVPVLSSF-NMLRGVLQARRKEKQ 535
>ref|XP_311969.1| ENSANGP00000010967 [Anopheles gambiae]
 gb|EAA07616.1| ENSANGP00000010967 [Anopheles gambiae str. PEST]
          Length = 590

 Score =  535 bits (1366), Expect = e-150
 Identities = 128/560 (22%), Positives = 214/560 (37%), Gaps = 71/560 (12%)

Query: 18  AIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAGGKSCRPVFPLEIRFE 77
                     AF  +I+ +G P+WWKTT +YR  LP  E+             P++  F 
Sbjct: 53  DAAIHIYATIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNED-------PIKATF- 104

Query: 78  TPSLPDVEAQQLLRITQHTLDDLNEFTAHHLRLKLAEDEAEQTTQKADTALTVRLQAQGD 137
              L    A++   ++        +     L L+L                   L    D
Sbjct: 105 RLGLYCQSAERREILSFELRKKFEQNFVFELDLQL-------------------LSLDAD 145

Query: 138 LTGPRSELRTDTTMLDIFYPPSQ-----IPPPSSPNSPLASFIAAELQHLFSEEKAIMSH 192
                         L   YP +      I  P   +  L   + ++     SE     S 
Sbjct: 146 TLSTIKTPARLEAELQKHYPSTLGHFLFIEWPKLDDDIL---VTSDRTAFISESA---SS 199

Query: 193 ILSNNAIGNYAVSTPNSLAHQISPQLAESITKRLRRSMKYAE---TYHLAFSLFTPGPAP 249
           + +   + ++ +      A   S    +++  R  R +  A     Y +  ++  P P  
Sbjct: 200 LKAYQVLSSWLLQEYKLKAILGSRDAQQTLMGRQLR-LNSAPLQAQYEVLITVLNPRPEL 258

Query: 250 SS--WDIESAVQGYISPIVQALSPISNFTVDTQVQL----YATFSPTTPVPEYDETQGVW 303
               W+  +A + YI P +  LS I+NFT+ TQ           +    VP+  +    +
Sbjct: 259 QKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQVGLEGAGAQPKQVPDDSKLGRHY 318

Query: 304 TLKEENLSAFINAAEWPLNPSIGSGPTINFILYVPAPSQSPLLVQESKAT---------- 353
            L E++L   I + E  L   I   P I+ ++YVP  +Q+PL +                
Sbjct: 319 ALAEDSLPHIITSLEKKLGTQITDNPCIHLVVYVPPCAQAPLRIYRKDGQRAAPLASNVE 378

Query: 354 SWIIPQWGGVFILNPPLSTTDG------------SAQSNPPHLPRDALRAPFLTFSHQLL 401
           ++   +WGG+   NP  +T                AQ   P L     +   L  +  LL
Sbjct: 379 AFTSAKWGGIVFANPAEATCVRYMETEQFSDVYIHAQDVMPVLLYQLRKIFDLENNTPLL 438

Query: 402 SLLGTP-STPPSLPFRLQTLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVA 460
                P ST     + + T +R     ++ SA++T+ SL +L   +  I I   V +++ 
Sbjct: 439 DATLVPYSTIEPRAWEVDTFMRTNTIYLVHSATTTLQSLIQLLGGIEYIVINDEVGAAIQ 498

Query: 461 TTLSHLSSSCESLQNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLL 520
           T    + ++ + L+ G+ Q A   AR A   AER+FF+ SM+  +YFPDE K A+Y+PL 
Sbjct: 499 TAHRKIVAAKQQLRAGRLQQAALLAREAYTSAERAFFDPSMLALLYFPDEQKYAIYIPLF 558

Query: 521 GPIGVPLVLGLLKEVKRVVT 540
            PI +P+V      +K    
Sbjct: 559 LPIMIPVVFSFNTILKYFRK 578
>ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Saccharomyces
           cerevisiae]
 sp|Q04080|GP17_YEAST GPI transamidase component GPI17
 pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
 gb|AAB64866.1| Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
          Length = 534

 Score =  535 bits (1365), Expect = e-150
 Identities = 137/558 (24%), Positives = 226/558 (39%), Gaps = 84/558 (15%)

Query: 17  SAIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQ--EMLDWAGGKSCRPVFPLEI 74
           S    R  V   F A+ LFLG P+W+K T++YRA LPI   E L     +    V P+ +
Sbjct: 2   SNANLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYV 61

Query: 75  RFETPSLPDVEAQQLLRITQHTLDDLNEFTAHHLRLKLAEDEAEQTTQKADTALTVRLQA 134
           + +T   PDV     ++   H L+   +     L++    +  EQ   + +    V L+ 
Sbjct: 62  KSDTYRFPDVHDAIQVQ-VNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKL 120

Query: 135 QGDLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAAEL-QHLFSEEKAIMSHI 193
             +  G  S   T  T+  ++Y  + +      ++ L  F+A  L +H F  E   ++  
Sbjct: 121 D-EFIGYSSAYDTKETL--VYYDDAAV-----LSNDLPFFVAQTLVEHTFQLEWTHLNKT 172

Query: 194 LSNNAIGNYAVSTPNSLAHQISPQLAESITKRLRRSMKYAETYHLAFSLFTPGPAPSSWD 253
                     VST N +A    P +                  HL+ +L +    P +W+
Sbjct: 173 CE-------GVSTNNDVAISYDPNI------------------HLSVTLLSGDGNPVAWE 207

Query: 254 IESAVQGYISPIVQALSPISNFTVDTQVQL------YATFSPTTPVPEYDETQGVWTLKE 307
           IE  +  Y SP  + LSP+ NFTVD+ +        ++     T V  +D +  +     
Sbjct: 208 IEPTLTDYFSPFRKFLSPLVNFTVDSSIVYHNDLNLHSLNGSCTSVTWFDLSHTI----- 262

Query: 308 ENLSAFINAAEWPLNPSIGSGPTINFILYVPAPSQSP---LLVQESKA---------TSW 355
            +LS   + A +P + +      +N  +  P+ S SP     +  ++           S+
Sbjct: 263 -DLSELSSMAYYPEDSA------LNLAIVFPSASSSPDGLAFINGTRISDEITTLDWNSY 315

Query: 356 IIPQWGGVFILNPPLSTTDGSAQSNPPHLPRDALRAPFLTFSHQLLSLLGTPSTPPSLPF 415
           ++PQWG + I   PL      ++        D L      F+  +  LLG       L  
Sbjct: 316 LVPQWGVIIINKMPLKPNSVISE--------DYLEPMMYRFATDIFQLLGLTEGSQDLLS 367

Query: 416 RLQTLIRIRAATVLLS---ASSTMGSLARLTESLPSIPIPATVASSVATTLSHLSSSCES 472
              T+   +  T+L +   A+ T+ SL +LT+    + IP  V+ +V   L       + 
Sbjct: 368 PYITIDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDL 427

Query: 473 LQNGQ------FQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVP 526
           L +        +  AL  +       E++FF   MV Q +FP EH +AVYLPLLGPI   
Sbjct: 428 LNDPGKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAV 487

Query: 527 LVLGLLKEVKRVVTGWKA 544
           +  G    +K      K 
Sbjct: 488 MFFGFYNVMKEKNQKSKK 505
>ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe]
 sp|Q10351|PIGS_SCHPO GPI transamidase component PIG-S homolog
 pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA93813.1| SPAC1F12.09 [Schizosaccharomyces pombe]
          Length = 554

 Score =  458 bits (1168), Expect = e-127
 Identities = 124/506 (24%), Positives = 206/506 (40%), Gaps = 71/506 (14%)

Query: 68  PVFPL---EIRFETPSLPDVEAQQLLRITQHTLDDLNEFTAHHLRLKLAEDEAEQTTQKA 124
              P+      +E  SLP  + +      Q            HLR               
Sbjct: 85  LAIPVWWKTTHYERSSLPFEDMENAPSTVQ-----------THLRFSPTF-RILDDKGNN 132

Query: 125 DTALTVRLQAQGDLTGPRSELRTDTTMLDIFYPPSQIPPPSSPNSPLASFIAAELQHLFS 184
            T               +  L  +  +    Y    +         +    + +LQ  + 
Sbjct: 133 LT------------KEVQKVLEAEPQIY--SYNLKVLEDDPVDYR-IVLRESTDLQWFWD 177

Query: 185 EEKAIMSHILSNNAIGNYAVSTPNSLAHQISPQLAESITKRLR-------------RSMK 231
           E   I+     +      A+   N L    SPQ+ E  +K  R             R+++
Sbjct: 178 ENNFIID--TPSKGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSSTVEPSRAETKRTVQ 235

Query: 232 YAETYHLAFSLFT--PGPAPSSWDIESAVQGYISPIVQALSPISNFTVDTQVQLYATFSP 289
           ++  Y +  SL        P +WDIE+A+Q Y +P+++ L+ ++   ++TQ+Q +   + 
Sbjct: 236 FSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQYFVEDA- 294

Query: 290 TTPVPEYDETQGVWTLKEENLSAFINAAEW--PLNPSIGSGPTINFILYVPAPSQSPLLV 347
                E       +  K  +L   +N  E     +P I   PTI+F+LYVP+P   PL +
Sbjct: 295 -----EAYIKDDKFCTKHADLPNLVNNFEKYLSFSPHIRE-PTIHFVLYVPSPQIQPLWL 348

Query: 348 QESKA-----TSWIIPQWGGVFILNPPLSTTDGSAQSNPPHLPRDALRAPFLTFSHQLLS 402
           +   +      S ++PQWG +  +N   + T+         L    L+  F   S  LL 
Sbjct: 349 ENEDSNIIPTNSMLLPQWGSITTIN--FNVTEKKL------LHDVDLKDYFRVISRDLLL 400

Query: 403 LLGTPSTP-PSLPFRL-QTLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVA 460
           LLG    P  SL   L   L+R R A   + AS T+ +LA+L  S+ S+ +P  +   V 
Sbjct: 401 LLGINDVPVSSLSATLADRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVK 460

Query: 461 TTLSHLSSSCESLQNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLL 520
            TL  L  + ++L       AL+ +  A ++++ + F  SMV  +YFPDE K  +Y PL 
Sbjct: 461 DTLLSLDMAYKALSQNNLNEALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLF 520

Query: 521 GPIGVPLVLGLLKEVKRVVTGWKARR 546
            PI +PL++  +KEVK ++   K  R
Sbjct: 521 APILIPLLISFIKEVKDMLRERKLHR 546
 Score = 45.9 bits (108), Expect = 0.003
 Identities = 26/110 (23%), Positives = 47/110 (42%), Gaps = 10/110 (9%)

Query: 6   SASTLPPPEKPSAIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQEMLDWAGGKS 65
           S   L  PEK      +   + +F+ VI+ L  P+WWKTT   R+ LP ++M +      
Sbjct: 60  SNFQLNKPEK----SLKRYALLSFY-VIILLAIPVWWKTTHYERSSLPFEDMENAPSTVQ 114

Query: 66  CRPVFPLEIRFETPSLPDVEAQQLLRITQHTLDDLNEFTAHHLRLKLAED 115
               F       T  + D +   L +  Q  L+   +  +++L++   + 
Sbjct: 115 THLRF-----SPTFRILDDKGNNLTKEVQKVLEAEPQIYSYNLKVLEDDP 159
>ref|NP_187374.2| expressed protein [Arabidopsis thaliana]
 gb|AAL32707.1| Unknown protein [Arabidopsis thaliana]
 gb|AAM91117.1| unknown protein [Arabidopsis thaliana]
          Length = 599

 Score =  391 bits (995), Expect = e-107
 Identities = 100/346 (28%), Positives = 156/346 (44%), Gaps = 32/346 (9%)

Query: 226 LRRSMKYAETYHLAFSLFTPGPAP--SSWDIESAVQGYISPIVQALSPISNFTVDTQVQL 283
               +       L+FSL    P      WD +   +  ++P+ +AL+PI+N TV++QV  
Sbjct: 248 EFMPVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLY 307

Query: 284 YATFSPTTPVPEYDETQGVWTLKEENLSAFINAAEWPLNPSI---GSGPTINFILYVPAP 340
           +    P +    +D+    +  +  +L  F+N+ EW L+ S    G    + F++Y+P+ 
Sbjct: 308 HT---PKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSG 364

Query: 341 SQSPLLVQE-----SKATSWIIPQWGGVFILNPPLSTTDGSAQSNPPHLPRD--ALRAPF 393
            + PLL+Q      SK   +I P WGGV + NP     D  + S      +D   +   F
Sbjct: 365 KECPLLLQLPNVEISKTNGFISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEIF 424

Query: 394 LTFSHQLLSL---------LGTPSTPPSL----PFRLQTLIRIRAATVLLSASSTMGSLA 440
           L    QL            LGT     S      + L  L R      L S S+T+GSL+
Sbjct: 425 LGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLS 484

Query: 441 RLTESLPSIPIPATVASSVATTL--SHLSSSCESLQNGQFQAALASARVAEAEAERSFFE 498
           RL  SLP + I   +   V  +L  + L+ S  SL  G + ++ +S+R A + AE +FF 
Sbjct: 485 RLVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASL--GGYSSSASSSREARSLAENAFFH 542

Query: 499 KSMVGQMYFPDEHKVAVYLPLLGPIGVPLVLGLLKEVKRVVTGWKA 544
            S++   YF  EH  AVY P   P+   +VL  ++E KR       
Sbjct: 543 PSIMSVSYFSYEHCFAVYSPFFLPVVGHVVLAAVREWKRYKQEKAK 588
 Score = 40.1 bits (93), Expect = 0.18
 Identities = 16/67 (23%), Positives = 25/67 (36%), Gaps = 1/67 (1%)

Query: 35  FLGFPIWWKTTSIYRAELPIQEMLDWAGGKSCRPV-FPLEIRFETPSLPDVEAQQLLRIT 93
            LG P  WK+  IYR+ LP  ++   +      P+ FP             +   L    
Sbjct: 42  LLGVPFLWKSVEIYRSPLPFHDIDSLSDQLESTPLRFPCNFHAVFVGFRSTDPDNLRSQI 101

Query: 94  QHTLDDL 100
           Q  ++ L
Sbjct: 102 QDGINQL 108
>gb|AAF20228.1| unknown protein [Arabidopsis thaliana]
          Length = 605

 Score =  352 bits (895), Expect = 2e-95
 Identities = 93/318 (29%), Positives = 145/318 (45%), Gaps = 32/318 (10%)

Query: 226 LRRSMKYAETYHLAFSLFTPGPAP--SSWDIESAVQGYISPIVQALSPISNFTVDTQVQL 283
               +       L+FSL    P      WD +   +  ++P+ +AL+PI+N TV++QV  
Sbjct: 271 EFMPVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLY 330

Query: 284 YATFSPTTPVPEYDETQGVWTLKEENLSAFINAAEWPLNPSI---GSGPTINFILYVPAP 340
           +    P +    +D+    +  +  +L  F+N+ EW L+ S    G    + F++Y+P+ 
Sbjct: 331 HT---PKSSFSSWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSG 387

Query: 341 SQSPLLVQE-----SKATSWIIPQWGGVFILNPPLSTTDGSAQSNPPHLPRD--ALRAPF 393
            + PLL+Q      SK   +I P WGGV + NP     D  + S      +D   +   F
Sbjct: 388 KECPLLLQLPNVEISKTNGFISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEIF 447

Query: 394 LTFSHQLLSL---------LGTPSTPPSL----PFRLQTLIRIRAATVLLSASSTMGSLA 440
           L    QL            LGT     S      + L  L R      L S S+T+GSL+
Sbjct: 448 LGQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLS 507

Query: 441 RLTESLPSIPIPATVASSVATTL--SHLSSSCESLQNGQFQAALASARVAEAEAERSFFE 498
           RL  SLP + I   +   V  +L  + L+ S  SL  G + ++ +S+R A + AE +FF 
Sbjct: 508 RLVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASL--GGYSSSASSSREARSLAENAFFH 565

Query: 499 KSMVGQMYFPDEHKVAVY 516
            S++   YF  EH  AVY
Sbjct: 566 PSIMSVSYFSYEHCFAVY 583
 Score = 36.9 bits (85), Expect = 1.4
 Identities = 15/65 (23%), Positives = 24/65 (36%), Gaps = 1/65 (1%)

Query: 37  GFPIWWKTTSIYRAELPIQEMLDWAGGKSCRPV-FPLEIRFETPSLPDVEAQQLLRITQH 95
           G P  WK+  IYR+ LP  ++   +      P+ FP             +   L    Q 
Sbjct: 67  GVPFLWKSVEIYRSPLPFHDIDSLSDQLESTPLRFPCNFHAVFVGFRSTDPDNLRSQIQD 126

Query: 96  TLDDL 100
            ++ L
Sbjct: 127 GINQL 131
>gb|AAH01319.2| PIGS protein [Homo sapiens]
          Length = 243

 Score =  287 bits (728), Expect = 5e-76
 Identities = 69/241 (28%), Positives = 119/241 (48%), Gaps = 21/241 (8%)

Query: 322 NPSIGSGPTINFILYVPAPSQSPLLVQESKA-----TSWIIPQWGGVFILNPPLSTTDGS 376
           + +    P +NF+LYVP  + SPL +Q+         ++  P+WGG+ + N    T + S
Sbjct: 1   SSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDSKTYNAS 60

Query: 377 AQSNPPHLPRDALRAPFLTFSHQLLSLLGT-------------PSTPPSLPFRLQTLIRI 423
               P  +  D +R     F  QL  L G              P++   + + L  L+  
Sbjct: 61  VL--PVRVEVDMVR-VMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWA 117

Query: 424 RAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSHLSSSCESLQNGQFQAALA 483
           R+   L +A++T+ SLA+L   + +I I   VAS V   ++ +  S E L +G   +A  
Sbjct: 118 RSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFV 177

Query: 484 SARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVPLVLGLLKEVKRVVTGWK 543
           +++ A   +E +FF+ S++  +YFPD+ K A+Y+PL  P+ VP++L L+K        W+
Sbjct: 178 ASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRKSWR 237

Query: 544 A 544
            
Sbjct: 238 K 238
>dbj|BAB92563.1| P0497A05.6 [Oryza sativa (japonica cultivar-group)]
          Length = 557

 Score =  274 bits (696), Expect = 3e-72
 Identities = 71/264 (26%), Positives = 116/264 (43%), Gaps = 39/264 (14%)

Query: 224 KRLRRSMKYAETYHLAFSLFTPGPAP--SSWDIESAVQGYISPIVQALSPISNFTVDTQV 281
           KR    +       L+FSL    P+     W+ E+  Q  ++P+++AL PI+N  +++QV
Sbjct: 261 KREFMPVGSDGNIVLSFSLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQV 320

Query: 282 QLYATFSPTTPVPEYDETQGVWTLKEENLSAFINAAEWPLNPSI---GSGPTINFILYVP 338
             +    P +     D+  G   L   ++  F+N+ EW L+ SI   G    + F++Y+P
Sbjct: 321 LYHT---PKSSYSYSDDKLGGNVLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYIP 377

Query: 339 APSQSPLLVQESKA-----TSWIIPQWGGVFILNPPLSTTDGSAQSNPPHLPRDALRAPF 393
           +  + PL +Q          ++I P WGGV I NPP     G +  + PH   D + +  
Sbjct: 378 SARECPLYLQLPDGELSKTNAFISPMWGGVVIWNPP-----GCSFGSKPHGALDKMSSEE 432

Query: 394 LT-----FSHQLLSLLGTPST----------------PPSLPFRLQTLIRIRAATVLLSA 432
           L      F  QL  L G  S+                     + L  L R  A + LLS 
Sbjct: 433 LMETIEIFIGQLRQLFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLLYRHHACSNLLSC 492

Query: 433 SSTMGSLARLTESLPSIPIPATVA 456
            +T+ SL+ L +SLP + +   + 
Sbjct: 493 LTTLESLSSLVQSLPRMIVMDEIG 516
 Score = 36.9 bits (85), Expect = 1.4
 Identities = 13/66 (19%), Positives = 27/66 (40%), Gaps = 8/66 (12%)

Query: 3  SKLSASTL-------PPPEKPSAIQTRSKVVAAFWAVILFLGFPIWWKTTSIYRAELPIQ 55
          +  ++S         P P      + R  + A+   +   +G P+  K+T I+R+ LP  
Sbjct: 27 APSTSSPSDADGGGKPSPRTSKPGRKRLVLTAS-VLLSFLIGLPLLLKSTEIHRSPLPSD 85

Query: 56 EMLDWA 61
           +   +
Sbjct: 86 AIAALS 91
 Score = 35.0 bits (80), Expect = 6.1
 Identities = 7/27 (25%), Positives = 16/27 (58%)

Query: 518 PLLGPIGVPLVLGLLKEVKRVVTGWKA 544
           P   P+ + ++L ++KE+KR +   + 
Sbjct: 519 PFFAPVSLHVLLAVMKELKRYMVERRK 545
>ref|XP_142436.3| similar to phosphatidylinositol glycan class S [Mus musculus]
          Length = 225

 Score =  259 bits (657), Expect = 1e-67
 Identities = 65/219 (29%), Positives = 97/219 (43%), Gaps = 23/219 (10%)

Query: 162 PPPSSPNSPLASFIAAELQHLFSEEKAIMSHILSNNAIGNYAVS-------TPNSLAHQI 214
           P    P   + S+I  E   +       + H  + N IG   V        T + LA  +
Sbjct: 10  PSLCPPAQDMMSYIGPERTAVVRG----LIHREAFNIIGRRIVQVAQAMSLTEDVLAAAL 65

Query: 215 SPQLAE-SITKRLRRSMKYAETYHLAFSLFTPGPAPSS--WDIESAVQGYISPIVQALSP 271
           +  L E   +   RR +K +  Y + FSL  P P      WDIE AVQ ++ P +  LS 
Sbjct: 66  ADHLPEDKWSSDKRRPLKSSLGYEITFSLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSV 125

Query: 272 ISNFTVDTQVQLYATFSPTTPVPEYDETQGVWTLKEENLSAFINAAEWPLNPSIGS-GPT 330
             NF+VD+Q+  YA        P +D     ++L   +L   IN  E  L  S  S  P 
Sbjct: 126 AGNFSVDSQILYYAMLGVN---PRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYPV 182

Query: 331 INFILYVPAPSQSPLLVQESKA-----TSWIIPQWGGVF 364
           ++F+LYVP  + SPL +Q+         ++  P+WGG+ 
Sbjct: 183 LHFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIM 221
>dbj|BAB70938.1| unnamed protein product [Homo sapiens]
          Length = 184

 Score =  207 bits (524), Expect = 5e-52
 Identities = 49/177 (27%), Positives = 86/177 (47%), Gaps = 14/177 (7%)

Query: 381 PPHLPRDALRAPFLTFSHQLLSLLGT-------------PSTPPSLPFRLQTLIRIRAAT 427
           P  +  D +R     F  QL  L G              P++   + + L  L+  R+  
Sbjct: 4   PVRVEVDMVR-VMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVE 62

Query: 428 VLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSHLSSSCESLQNGQFQAALASARV 487
            L +A++T+ SLA+L   + +I I   VAS V   ++ +  S E L +G   +A  +++ 
Sbjct: 63  NLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQE 122

Query: 488 AEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVPLVLGLLKEVKRVVTGWKA 544
               +E +FF+ S++  +YFPD+ K  +Y+PL  P+ VP++L L+K        W+ 
Sbjct: 123 VVTSSELAFFDPSLLHLLYFPDDQKFPIYIPLFLPMAVPILLSLVKLFLETRKSWRK 179
>emb|CAE45912.1| hypothetical protein [Homo sapiens]
          Length = 162

 Score =  195 bits (493), Expect = 2e-48
 Identities = 45/142 (31%), Positives = 79/142 (54%)

Query: 403 LLGTPSTPPSLPFRLQTLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATT 462
           LL  P++   + + L  L+  R+   L +A++T+ SLA+L   + +I I   VAS V   
Sbjct: 16  LLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKA 75

Query: 463 LSHLSSSCESLQNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGP 522
           ++ +  S E L +G   +A  +++ A   +E  FF+ S++  +YFPD+ K A+Y+PL  P
Sbjct: 76  VAAVQKSAEELASGHLASAFVASQEAVTSSELVFFDPSLLHLLYFPDDQKFAIYIPLFLP 135

Query: 523 IGVPLVLGLLKEVKRVVTGWKA 544
           + VP++L L+K        W+ 
Sbjct: 136 MAVPILLSLVKIFLETRKSWRK 157
>ref|NP_501852.1| phosphatidylinositol glycan class S like (4K912) [Caenorhabditis
           elegans]
 pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
 emb|CAA93093.1| Hypothetical protein T14G10.7 [Caenorhabditis elegans]
          Length = 544

 Score =  132 bits (331), Expect = 2e-29
 Identities = 23/114 (20%), Positives = 50/114 (43%), Gaps = 4/114 (3%)

Query: 414 PFRLQTLIRIRAATVLLSASSTMGSLARLTESLPSIPIPATVASSVATTLSHLSSSCESL 473
            + +       +    + A+S + +L +L + + +I I   VA      +  L    + +
Sbjct: 418 QWEVARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVADRATRAVEIL---NDVV 474

Query: 474 QNGQFQAALASARVAEAEAERSFFEKSMVGQMYFPDEHKVAVYLPLLGPIGVPL 527
           + G+          A   A+ +  + S++  +YFP + + AV +PL  PI +P+
Sbjct: 475 RPGRMLD-FERLLEARRLADSANSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPI 527
 Score = 43.2 bits (101), Expect = 0.022
 Identities = 49/363 (13%), Positives = 106/363 (28%), Gaps = 54/363 (14%)

Query: 25  VVAAFWAVILFLGFPIWWKTTSIYRAE---LPIQEMLDWAGGKSCRPVFPLEIRFETPSL 81
              +F  ++ F+G P+W  TTS YR+     P  + +            P+ + F   S 
Sbjct: 48  ASLSFVILVFFIGMPMWHFTTSTYRSNFPTFPSNQSI----------TVPIRVIFVITS- 96

Query: 82  PDVEAQQLLRITQHTLDDLNEFTAHHLRLKLAEDEAEQT------TQKADTALTVRLQAQ 135
              + +  ++     +++L         +   +             +  +   +++++  
Sbjct: 97  --EDLRNDVKT---LMNELEVNMTKKEVVAPLDFTWTTQFIGLRSFEHLEQDHSIQIEEN 151

Query: 136 GDLTGPRSELRTDTTMLDIFYPP----------SQIPPPSSPNSPLASFIAAELQHLFSE 185
            +       L  +       Y            + I  P+          +  +  L SE
Sbjct: 152 KEFFDVFVALVPEKE--WTHYSATRIQLGIGKWNFIQWPTKSEEK--QKASTRVTDLVSE 207

Query: 186 EKAIMSHILSNNAIGNYAVSTPNSLAHQISPQLAESITKRLRRSMKYAE---TYHLAFSL 242
               + H+   N+I    +              A  ++ + R     A     YH+    
Sbjct: 208 TLIDVPHL---NSIVRRDLRQKMQPWQI----AALPLSHQKRLVWDSAPLATNYHIQVIH 260

Query: 243 FTPGPA--PSSWDIESAVQGYISPIVQALSPISNFTVDTQVQLYATFSPTTPVPEYDETQ 300
                   P S   +      +    + +  I+N  V+   +    F PT      D  +
Sbjct: 261 LHENSEITPESLKEQEKTARALRKFAEKVKEITN--VEVSFEHLWDFEPTRGFLIKDVQE 318

Query: 301 GVWTLKEENLSAFINAAEWPLNPSIGSGPTINFILYVPAPSQSPLLVQESKATSWIIPQW 360
             W L    +   +   +  L  S+  G  + F++         L      +    +  W
Sbjct: 319 R-WALTHSAMEELVKKVDSQLQTSLQHGAVLRFVVLETVEPVIVLDDTGEDSHGVAVASW 377

Query: 361 GGV 363
           G V
Sbjct: 378 GAV 380
>ref|NP_701826.1| hypothetical protein [Plasmodium falciparum 3D7]
 gb|AAN36550.1| hypothetical protein PFL2330w [Plasmodium falciparum 3D7]
          Length = 746

 Score = 42.4 bits (99), Expect = 0.035
 Identities = 36/165 (21%), Positives = 60/165 (35%), Gaps = 16/165 (9%)

Query: 261 YISPIVQALSPISNFTVDTQVQ----LYATFSPTTPVPEYDETQGVWTLKEENLSAFINA 316
           YI P ++ L  I    +  Q+     +Y          E  E      +  + ++ F N 
Sbjct: 364 YIRPFIERLLYIYQINIYPQILSNINMYEISDNINLYIEEKEKGNARLIILDKITKFTNI 423

Query: 317 AEWPLNPSIGSGPT------INFILYVPAPSQSPLLVQESKAT----SWIIPQWGGVFIL 366
            +     ++ S PT      INF++  P   +       SK      +  I +WG ++I 
Sbjct: 424 FDEITFDNVLSKPTYEVPKNINFMVVFPNGDEIYFYNNLSKTNKIETAVSIAEWGTIYIN 483

Query: 367 NPPLSTTDGSAQSNPPHLPRDALRAPFL--TFSHQLLSLLGTPST 409
           N      D S  +N  +L   + +A  +   F     SLLG  S 
Sbjct: 484 NEFTQLKDRSNYNNKNNLVNISNQAHVISGIFISHFRSLLGLCSI 528
CPU time:   179.86 user secs.	    5.92 sys. secs	  185.78 total secs.

  Database: nr
    Posted date:  Nov 22, 2003  1:30 PM
  Number of letters in database: 504,497,942
  Number of sequences in database:  1,543,518
  
Lambda     K      H
   0.309    0.167    0.455 

Gapped
Lambda     K      H
   0.270   0.0584    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 555253879
Number of Sequences: 1543518
Number of extensions: 25755364
Number of successful extensions: 65313
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 65101
Number of HSP's gapped (non-prelim): 57
length of query: 551
length of database: 504,497,942
effective HSP length: 62
effective length of query: 489
effective length of database: 408,799,826
effective search space: 199903114914
effective search space used: 199903114914
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (20.9 bits)
S2: 78 (34.5 bits)