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GPI Anchor Biosynthesis Report: AAL27645.1 (PIG-S family, Drosophila melanogaster)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (536 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 15 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

gb|AAL27645.1| (AY061834) SD10476p [Drosophila melanogaster] 784 0.0
ref|NP_149975.1| (NM_033198) phosphatidylinositol glycan, class ... 600 e-170
ref|NP_010722.1| (NC_001136) Glycosyl Phosphatidyl Inositol 17; ... 582 e-165
gb|AAF56365.1| (AE003749) CG13644 gene product [Drosophila melan... 496 e-139
sp|Q10351|YDA9_SCHPO HYPOTHETICAL 63.9 KD PROTEIN C1F12.09 IN CH... 401 e-110
gb|AAL32707.1| (AY062629) Unknown protein [Arabidopsis thaliana] 392 e-108
ref|NP_187374.1| (NM_111598) unknown protein [Arabidopsis thalia... 376 e-103
ref|NP_501852.1| (NM_069451) T14G10.7.p [Caenorhabditis elegans]... 347 2e-94
gb|AAF56366.1| (AE003749) CG6031 gene product [Drosophila melano... 252 9e-66
dbj|BAB70938.1| (AK055512) unnamed protein product [Homo sapiens] 217 5e-55
ref|XP_091596.1| (XM_091596) similar to phosphatidylinositol gly... 171 3e-41
Sequences with E-value WORSE than threshold

ref|XP_082327.1| (XM_082327) CG18761 [Drosophila melanogaster] >... 40 0.11
Alignments
>gb|AAL27645.1| (AY061834) SD10476p [Drosophila melanogaster]
          Length = 536

 Score =  784 bits (2003), Expect = 0.0
 Identities = 536/536 (100%), Positives = 536/536 (100%)

Query: 1   METSSSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSL 60
           METSSSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSL
Sbjct: 1   METSSSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSL 60

Query: 61  SKIPIKTAVQVAIYTQQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAAL 120
           SKIPIKTAVQVAIYTQQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAAL
Sbjct: 61  SKIPIKTAVQVAIYTQQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAAL 120

Query: 121 EKLLLENHVQSVGDFMFIEWPKLQEELLLTTERSALMRSDTPSNKIAQLLHAKILQTYRI 180
           EKLLLENHVQSVGDFMFIEWPKLQEELLLTTERSALMRSDTPSNKIAQLLHAKILQTYRI
Sbjct: 121 EKLLLENHVQSVGDFMFIEWPKLQEELLLTTERSALMRSDTPSNKIAQLLHAKILQTYRI 180

Query: 181 NQILSTDERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVS 240
           NQILSTDERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVS
Sbjct: 181 NQILSTDERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVS 240

Query: 241 NYTVRSQWKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAIN 300
           NYTVRSQWKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAIN
Sbjct: 241 NYTVRSQWKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAIN 300

Query: 301 LVVYIPPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVP 360
           LVVYIPPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVP
Sbjct: 301 LVVYIPPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVP 360

Query: 361 YHVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHIST 420
           YHVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHIST
Sbjct: 361 YHVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHIST 420

Query: 421 ASSTLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVA 480
           ASSTLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVA
Sbjct: 421 ASSTLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVA 480

Query: 481 SERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSFNMLRGVLQARRKEKQS 536
           SERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSFNMLRGVLQARRKEKQS
Sbjct: 481 SERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSFNMLRGVLQARRKEKQS 536
>ref|NP_149975.1| (NM_033198) phosphatidylinositol glycan, class S [Homo sapiens]
 dbj|BAB60853.1| (AB057723) phosphatidyl inositol glycan class S [Homo sapiens]
          Length = 555

 Score =  600 bits (1532), Expect = e-170
 Identities = 146/531 (27%), Positives = 245/531 (45%), Gaps = 49/531 (9%)

Query: 25  ATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSKIPIKTAVQ-VAIYTQQTSRGQL 83
           A L F  V I +G+P+WW+TT  YR +LP ++I  L+ + ++  V    ++T+++     
Sbjct: 19  AALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLRLMVPVTVVFTRESVPLDD 78

Query: 84  L-------IAELQNAFSDNEIWSVEFKQL--SPTPMTQEA---HTPAALEKLLLENHVQ- 130
                   + E +            F++         +EA    +    E +L E   Q 
Sbjct: 79  QEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQEAEAMLDEPQEQA 138

Query: 131 --SVGDFMFIEWPKLQEELLLTT---ERSALMR---SDTPSNKIAQLL--HAKILQTYR- 179
             S+  ++  E   L  + +++    +R+A++R        N I + +   A+ +     
Sbjct: 139 EGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTED 198

Query: 180 -INQIL---------STDERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYI 229
            +   L         S ++R   KS      Y++  S+LNP P+     W+I  AV+ Y+
Sbjct: 199 VLAAALADHLPEDKWSAEKRRPLKSSL---GYEITFSLLNPDPKSHDVYWDIEGAVRRYV 255

Query: 230 EPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNL 289
           +P+L  +    N++V SQ  Y   +  + +     S     Y L   +LPH++  +   L
Sbjct: 256 QPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASS----SYYLDMHSLPHVINPVESRL 311

Query: 290 SASTTD-KPAINLVVYIPPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHV 348
            +S     P +N ++Y+P    +PL+I +     +  N   AF SP WGG ++ N     
Sbjct: 312 GSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATN---AFHSPRWGGIMVYNVDSKT 368

Query: 349 CLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEA 408
             A++     V   V     M+V L QL  L  I+         +          WE + 
Sbjct: 369 YNASVL---PVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDR 425

Query: 409 YLRRSAIRHISTASSTLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLA 468
            L   ++ +++TA++TL SL +LL +IS IVI D+V + +  + A +  +   L    LA
Sbjct: 426 LLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLA 485

Query: 469 DASVLAKRAFVASERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSS 519
            A V ++ A  +SE  FFD SLL  LYFPD+QK+AIYIPLFLP+ VP+L S
Sbjct: 486 SAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLS 536
>ref|NP_010722.1| (NC_001136) Glycosyl Phosphatidyl Inositol 17; Gpi17p
           [Saccharomyces cerevisiae]
 pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
 gb|AAB64866.1| (U33007) Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
          Length = 534

 Score =  582 bits (1486), Expect = e-165
 Identities = 118/528 (22%), Positives = 211/528 (39%), Gaps = 60/528 (11%)

Query: 29  FIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSK---------IPIKTAVQVAIYTQQTS 79
           F+ + + +GVP+W++ TTVYR +LP   I SL           IP+        +     
Sbjct: 14  FVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYVKSDTYRFPDVHD 73

Query: 80  RGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAALEKLLLENHVQSVGDFMFIE 139
             Q+ +  L N+      WS+   Q+ P   T E      +E    + HV ++    FI 
Sbjct: 74  AIQVQVNHLLNSQEQRVPWSL---QVLPYNETIE-----QMESEGNQFHVVTLKLDEFIG 125

Query: 140 WPKL--QEELLLTTERSALMRSDTPSNKIAQLL--HAKILQTYRINQILSTDERMGAKSE 195
           +      +E L+  + +A++ +D P   +AQ L  H   L+   +N+           + 
Sbjct: 126 YSSAYDTKETLVYYDDAAVLSNDLPFF-VAQTLVEHTFQLEWTHLNKTCEGVSTNNDVAI 184

Query: 196 APQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYRVAIE 255
           +  P   + V++L+         W I   +  Y  P+   +S + N+TV S   Y     
Sbjct: 185 SYDPNIHLSVTLLSGDGN--PVAWEIEPTLTDYFSPFRKFLSPLVNFTVDSSIVYH---- 238

Query: 256 ADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCHIAP--L 313
                +   S  G   ++    L H +     +  A   +  A+NL +  P    +P  L
Sbjct: 239 ---NDLNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSASSSPDGL 295

Query: 314 HIYNSK--DQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNMQV 371
              N       +T  D ++++ P WG  II   P              P  V + D ++ 
Sbjct: 296 AFINGTRISDEITTLDWNSYLVPQWGVIIINKMPLK------------PNSVISEDYLEP 343

Query: 372 MLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSLIKL 431
           M+ +     DI    Q+ G+     + L P     +++ R + ++++  A+ TL SL+KL
Sbjct: 344 MMYRFAT--DI---FQLLGLTEGSQDLLSP-YITIDSFKRLTILQNLDKATETLWSLVKL 397

Query: 432 LDQISYIVIDDEVGAAITNSY------ADILAAKAALLEHRLADASVLAKRAFVASERGF 485
             Q   + I  EV   +  +        D+L       +    +A  L+       E+ F
Sbjct: 398 TQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNELVKLCEKAF 457

Query: 486 FDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSF-NMLRGVLQARRK 532
           F+  ++ Q +FP E   A+Y+PL  PI   +   F N+++   Q  +K
Sbjct: 458 FNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMKEKNQKSKK 505
>gb|AAF56365.1| (AE003749) CG13644 gene product [Drosophila melanogaster]
          Length = 347

 Score =  496 bits (1265), Expect = e-139
 Identities = 347/347 (100%), Positives = 347/347 (100%)

Query: 190 MGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWK 249
           MGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWK
Sbjct: 1   MGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWK 60

Query: 250 YRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCH 309
           YRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCH
Sbjct: 61  YRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPPCH 120

Query: 310 IAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNM 369
           IAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNM
Sbjct: 121 IAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNM 180

Query: 370 QVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSLI 429
           QVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSLI
Sbjct: 181 QVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTASSTLQSLI 240

Query: 430 KLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGFFDAS 489
           KLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGFFDAS
Sbjct: 241 KLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVASERGFFDAS 300

Query: 490 LLAQLYFPDEQKYAIYIPLFLPIMVPVLSSFNMLRGVLQARRKEKQS 536
           LLAQLYFPDEQKYAIYIPLFLPIMVPVLSSFNMLRGVLQARRKEKQS
Sbjct: 301 LLAQLYFPDEQKYAIYIPLFLPIMVPVLSSFNMLRGVLQARRKEKQS 347
>sp|Q10351|YDA9_SCHPO HYPOTHETICAL 63.9 KD PROTEIN C1F12.09 IN CHROMOSOME I
 pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 pir||T38067 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA93813.1| (Z69944) hypothetical protein [Schizosaccharomyces pombe]
          Length = 554

 Score =  401 bits (1021), Expect = e-110
 Identities = 86/364 (23%), Positives = 161/364 (43%), Gaps = 39/364 (10%)

Query: 173 KILQTY----RINQILSTDERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTY 228
           ++++ +    R +  +        ++    P Y V++S+L  +       W+I  A++ Y
Sbjct: 208 QVMEVWSKFTRFSSTVEPSRAETKRTVQFSPQYRVLLSLLVGEGNHEPINWDIENAIQKY 267

Query: 229 IEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQN 288
             P + +++ ++   + +Q +Y V  E     ++D     +H       LP+L+ +  + 
Sbjct: 268 FNPLIEQLASLAKLNIETQIQYFV--EDAEAYIKDDKFCTKH-----ADLPNLVNNFEKY 320

Query: 289 LSAST-TDKPAINLVVYIPPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEH 347
           LS S    +P I+ V+Y+P   I PL + N     +  N   + + P WG     N    
Sbjct: 321 LSFSPHIREPTIHFVLYVPSPQIQPLWLENEDSNIIPTN---SMLLPQWGSITTIN---- 373

Query: 348 VCLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYE 407
                   ++ + + V   D  +V+   L  LL I +++ +  +               +
Sbjct: 374 ----FNVTEKKLLHDVDLKDYFRVISRDLLLLLGI-NDVPVSSLSATLA----------D 418

Query: 408 AYLRRSAIRHISTASSTLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRL 467
             LR+        AS TLQ+L KL+  +  + +  E+   + ++   +  A  AL ++ L
Sbjct: 419 RLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNL 478

Query: 468 ADASVLAKRAFVASERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSF-----NM 522
            +A   +  AF  S+   F  S++  +YFPDE KY IY PLF PI++P+L SF     +M
Sbjct: 479 NEALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISFIKEVKDM 538

Query: 523 LRGV 526
           LR  
Sbjct: 539 LRER 542
 Score = 39.7 bits (92), Expect = 0.13
 Identities = 16/64 (25%), Positives = 30/64 (46%), Gaps = 3/64 (4%)

Query: 15  DKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSL-SKIPIKTA-VQVA 72
           +K E   +  A L+F V+I+ + +P+WW+TT   R +LP  ++ +  S +          
Sbjct: 65  NKPEKSLKRYALLSFYVIIL-LAIPVWWKTTHYERSSLPFEDMENAPSTVQTHLRFSPTF 123

Query: 73  IYTQ 76
               
Sbjct: 124 RILD 127
>gb|AAL32707.1| (AY062629) Unknown protein [Arabidopsis thaliana]
          Length = 599

 Score =  392 bits (997), Expect = e-108
 Identities = 84/322 (26%), Positives = 140/322 (43%), Gaps = 24/322 (7%)

Query: 203 VIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVR 262
           +  S+LN  PR     W+     +  + P    ++ ++N TV SQ  Y     +      
Sbjct: 260 LSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSS----FS 315

Query: 263 DQSKLGRHYALQETALPHLLTSIAQNL--SASTTDKPAI-NLVVYIPPCHIAPLHIYNSK 319
              K  + Y  + + LP  + S   +L  SA  + +  I   VVYIP     PL +   +
Sbjct: 316 SWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLL---Q 372

Query: 320 DQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNM----QVMLDQ 375
              +  +  + FISP WGG I+ NP          D E+   +  +  ++    ++ L Q
Sbjct: 373 LPNVEISKTNGFISPMWGGVIVWNPGN-----CDKDSESPSRNTISLQDLEQIVEIFLGQ 427

Query: 376 LHKLLDISSELQ-MEGVKVVDIEQLEP--RRWEYEAYLRRSAIRHISTASSTLQSLIKLL 432
             +L    SE +   G+    I   E     WE +   R+    ++ + S+TL SL +L+
Sbjct: 428 FRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSRLV 487

Query: 433 DQISYIVIDDEVGAAITNSY-ADILAAKAALLEHRLADASVLAKRAFVASERGFFDASLL 491
             +  ++I DE+G  +  S  A  LA   A L    + AS   +   +A E  FF  S++
Sbjct: 488 RSLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLA-ENAFFHPSIM 546

Query: 492 AQLYFPDEQKYAIYIPLFLPIM 513
           +  YF  E  +A+Y P FLP++
Sbjct: 547 SVSYFSYEHCFAVYSPFFLPVV 568
 Score = 34.6 bits (79), Expect = 3.6
 Identities = 12/32 (37%), Positives = 19/32 (58%)

Query: 30 IVVIIGIGVPMWWRTTTVYRVNLPSTEILSLS 61
          ++    +GVP  W++  +YR  LP  +I SLS
Sbjct: 37 VLFSFLLGVPFLWKSVEIYRSPLPFHDIDSLS 68
>ref|NP_187374.1| (NM_111598) unknown protein [Arabidopsis thaliana]
 gb|AAF20228.1|AC012395_15 (AC012395) unknown protein [Arabidopsis thaliana]
          Length = 605

 Score =  376 bits (956), Expect = e-103
 Identities = 80/315 (25%), Positives = 134/315 (42%), Gaps = 24/315 (7%)

Query: 203 VIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVR 262
           +  S+LN  PR     W+     +  + P    ++ ++N TV SQ  Y     +      
Sbjct: 283 LSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSS----FS 338

Query: 263 DQSKLGRHYALQETALPHLLTSIAQNL--SASTTDKPAI-NLVVYIPPCHIAPLHIYNSK 319
              K  + Y  + + LP  + S   +L  SA  + +  I   VVYIP     PL +   +
Sbjct: 339 SWDKKLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLL---Q 395

Query: 320 DQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNM----QVMLDQ 375
              +  +  + FISP WGG I+ NP          D E+   +  +  ++    ++ L Q
Sbjct: 396 LPNVEISKTNGFISPMWGGVIVWNPGN-----CDKDSESPSRNTISLQDLEQIVEIFLGQ 450

Query: 376 LHKLLDISSELQ-MEGVKVVDIEQLEP--RRWEYEAYLRRSAIRHISTASSTLQSLIKLL 432
             +L    SE +   G+    I   E     WE +   R+    ++ + S+TL SL +L+
Sbjct: 451 FRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSRLV 510

Query: 433 DQISYIVIDDEVGAAITNSY-ADILAAKAALLEHRLADASVLAKRAFVASERGFFDASLL 491
             +  ++I DE+G  +  S  A  LA   A L    + AS   +   +A E  FF  S++
Sbjct: 511 RSLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLA-ENAFFHPSIM 569

Query: 492 AQLYFPDEQKYAIYI 506
           +  YF  E  +A+Y 
Sbjct: 570 SVSYFSYEHCFAVYS 584
>ref|NP_501852.1| (NM_069451) T14G10.7.p [Caenorhabditis elegans]
 pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
 emb|CAA93093.1| (Z68880) predicted using Genefinder~cDNA EST EMBL:M89453 comes from
           this gene~cDNA EST yk111h3.3 comes from this gene~cDNA
           EST yk111h3.5 comes from this gene~cDNA EST yk457b10.3
           comes from this gene~cDNA EST yk457b10.5 comes from this
           gene~cDNA EST yk3>
          Length = 544

 Score =  347 bits (883), Expect = 2e-94
 Identities = 69/321 (21%), Positives = 131/321 (40%), Gaps = 44/321 (13%)

Query: 18  EDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSKIPIKTAVQVAIYTQQ 77
           E  +++ A+L+F++++  IG+PMW  TT+ YR N P+        +PI+    + + T +
Sbjct: 41  ERPYKLAASLSFVILVFFIGMPMWHFTTSTYRSNFPTFPSNQSITVPIRV---IFVITSE 97

Query: 78  TSRGQL--LIAELQNAFSDNEI-------WSVEFKQLSPTPMTQEAHTPAALEK------ 122
             R  +  L+ EL+   +  E+       W+ +F  L      ++ H+    E       
Sbjct: 98  DLRNDVKTLMNELEVNMTKKEVVAPLDFTWTTQFIGLRSFEHLEQDHSIQIEENKEFFDV 157

Query: 123 ---LLLE--------NHVQ-SVGDFMFIEWPKLQEELLLTTERSALMRSDT----PS-NK 165
              L+ E          +Q  +G + FI+WP   EE    + R   + S+T    P  N 
Sbjct: 158 FVALVPEKEWTHYSATRIQLGIGKWNFIQWPTKSEEKQKASTRVTDLVSETLIDVPHLNS 217

Query: 166 IAQLLHAKILQTYRINQI-LSTDERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMA 224
           I +    + +Q ++I  + LS  +R+   S      Y + V  L+    +T         
Sbjct: 218 IVRRDLRQKMQPWQIAALPLSHQKRLVWDSAPLATNYHIQVIHLHENSEITPESLKEQEK 277

Query: 225 VKTYIEPWLAKVSGVSNYTVRSQ--WKYRVAIEADLKQVRDQSKLGRHYALQETALPHLL 282
               +  +  KV  ++N  V  +  W +       +K V+++      +AL  +A+  L+
Sbjct: 278 TARALRKFAEKVKEITNVEVSFEHLWDFEPTRGFLIKDVQER------WALTHSAMEELV 331

Query: 283 TSIAQNLSASTTDKPAINLVV 303
             +   L  S      +  VV
Sbjct: 332 KKVDSQLQTSLQHGAVLRFVV 352
 Score =  137 bits (343), Expect = 4e-31
 Identities = 40/117 (34%), Positives = 64/117 (54%), Gaps = 10/117 (8%)

Query: 403 RWEYEAYLRRSAIRHISTASSTLQSLIKLLDQISYIVIDDEVGAAITNSYA---DILAAK 459
           +WE      R+++ +   A+S +++L +L  +IS IVI+D+V    T +     D++   
Sbjct: 418 QWEVARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVADRATRAVEILNDVVRPG 477

Query: 460 AALLEHRLADASVLAKRAFVASERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPV 516
             L   RL +A  LA  A         D SLLA LYFP +Q+ A+ IPL +PI++P+
Sbjct: 478 RMLDFERLLEARRLADSANS-------DHSLLAMLYFPMDQRTAVLIPLAVPIVLPI 527
>gb|AAF56366.1| (AE003749) CG6031 gene product [Drosophila melanogaster]
          Length = 164

 Score =  252 bits (639), Expect = 9e-66
 Identities = 161/161 (100%), Positives = 161/161 (100%)

Query: 1   METSSSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSL 60
           METSSSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSL
Sbjct: 1   METSSSSPVKPRRKDKDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSL 60

Query: 61  SKIPIKTAVQVAIYTQQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAAL 120
           SKIPIKTAVQVAIYTQQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAAL
Sbjct: 61  SKIPIKTAVQVAIYTQQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMTQEAHTPAAL 120

Query: 121 EKLLLENHVQSVGDFMFIEWPKLQEELLLTTERSALMRSDT 161
           EKLLLENHVQSVGDFMFIEWPKLQEELLLTTERSALMRSDT
Sbjct: 121 EKLLLENHVQSVGDFMFIEWPKLQEELLLTTERSALMRSDT 161
>dbj|BAB70938.1| (AK055512) unnamed protein product [Homo sapiens]
          Length = 184

 Score =  217 bits (548), Expect = 5e-55
 Identities = 60/162 (37%), Positives = 86/162 (53%)

Query: 358 AVPYHVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRH 417
            V   V     M+V L QL  L  I+         +          WE +  L   ++ +
Sbjct: 4   PVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVEN 63

Query: 418 ISTASSTLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRA 477
           ++TA++TL SL +LL +IS IVI D+V + +  + A +  +   L    LA A V ++  
Sbjct: 64  LATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEV 123

Query: 478 FVASERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSS 519
             +SE  FFD SLL  LYFPD+QK+ IYIPLFLP+ VP+L S
Sbjct: 124 VTSSELAFFDPSLLHLLYFPDDQKFPIYIPLFLPMAVPILLS 165
>ref|XP_091596.1| (XM_091596) similar to phosphatidylinositol glycan, class S (H.
           sapiens) [Homo sapiens]
          Length = 204

 Score =  171 bits (430), Expect = 3e-41
 Identities = 38/181 (20%), Positives = 73/181 (39%), Gaps = 30/181 (16%)

Query: 95  NEIWSVEFKQL--SPTPMTQEA---HTPAALEKLLLENHVQ---SVGDFMFIEWPKLQEE 146
                  F++         +EA    +    E +L E   Q   S+  ++  E   L  +
Sbjct: 22  KMKIKCRFQKAYRRALDHEEEALSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQ 81

Query: 147 LLLTT---ERSALMR---SDTPSNKIAQLL--HAKILQTYR--INQIL---------STD 187
            +++    +R+A++R        N I + +   A+ +      +   L         S +
Sbjct: 82  DMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAE 141

Query: 188 ERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQ 247
           +R   KS      Y++  S+LNP P+     W+I  AV+ Y++P+L  +    N++V SQ
Sbjct: 142 KRRPLKSSL---GYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQ 198

Query: 248 W 248
            
Sbjct: 199 V 199
>ref|XP_082327.1| (XM_082327) CG18761 [Drosophila melanogaster]
 gb|AAG22170.1| (AE003749) CG18761 gene product [Drosophila melanogaster]
 gb|AAL13751.1| (AY058522) LD22945p [Drosophila melanogaster]
          Length = 536

 Score = 39.7 bits (92), Expect = 0.11
 Identities = 19/19 (100%), Positives = 19/19 (100%)

Query: 1  METSSSSPVKPRRKDKDED 19
          METSSSSPVKPRRKDKDED
Sbjct: 1  METSSSSPVKPRRKDKDED 19
CPU time:   103.86 user secs.	    2.32 sys. secs	  106.18 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.310    0.163    0.438 

Gapped
Lambda     K      H
   0.270   0.0572    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 252848135
Number of Sequences: 887402
Number of extensions: 9568674
Number of successful extensions: 48782
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 48707
Number of HSP's gapped (non-prelim): 39
length of query: 536
length of database: 277,845,442
effective HSP length: 61
effective length of query: 475
effective length of database: 223,713,920
effective search space: 106264112000
effective search space used: 106264112000
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (20.9 bits)
S2: 75 (33.3 bits)