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GPI Anchor Biosynthesis Report: orf6.7723.prot (PIG-Q family, Candida albicans)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (648 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 21 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 613 e-174
ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 499 e-140
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 466 e-130
ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 446 e-124
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 415 e-115
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 414 e-114
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 414 e-114
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 410 e-113
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 407 e-112
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 404 e-111
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 386 e-106
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 369 e-101
emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 309 9e-83
emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 251 3e-65
emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 238 3e-61
ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 211 3e-53
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 149 1e-34
emb|CAB68123.2| (AL137080) hypothetical protein [Arabidopsis tha... 81 6e-14
dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:13331... 79 3e-13
pir||T45795 hypothetical protein F28O9.20 - Arabidopsis thaliana 74 5e-12
gb|AAH10094.1|AAH10094 (BC010094) Similar to phosphatidylinosito... 63 1e-08
Alignments
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
           - fission yeast (Schizosaccharomyces pombe)
 emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
           protein gpi1. [Schizosaccharomyces pombe]
          Length = 653

 Score =  613 bits (1565), Expect = e-174
 Identities = 168/647 (25%), Positives = 306/647 (46%), Gaps = 107/647 (16%)

Query: 1   MNSIQPPLQ---IYFPNDLKIHYQDQGYWLGYKL--NNIYIVFS----------LVVNDK 45
           MN    P++   IY+P    I Y D GY +G+K   N++ +V +          LV   K
Sbjct: 6   MNIASAPIEVKRIYWPKS-TISYTDSGYLVGWKYSTNDLIVVTTITSYQSFANFLVDYCK 64

Query: 46  DLKLPPELSDLAIIGTHNQKTTKQREGGYSNYDLNLIYDHTNQLLINQNQDS-------- 97
           +     ++  L I+GT N  +     G     D  +    + +L   +  DS        
Sbjct: 65  NQDQSVDIKSLCILGTFNC-SADILPGDKYEIDCWIKVQQSTELSHPRVFDSASTPLKIN 123

Query: 98  -YLIYFDPPNFKNLEYFSITP--------ILLQSMGPNLGKSQDDQLFNKLKQFEYQNPL 148
            ++IY+ PP  + +++ S+ P          +    P     Q  Q+   LK+ +   P 
Sbjct: 124 LHIIYYHPPQPRKMQFLSLEPLSLLLLKDSFINKSNPEYESMQHQQIL--LKKLKLHFPR 181

Query: 149 KTKKDDQSFISDDLVLDKINHLYRNRLL------YKRFHSNRSFRALNSKKFNFF-IIQN 201
           + +   +  +   L+   +N  +  R+L       K  +  R F  ++S  F FF  +  
Sbjct: 182 RKENSWKRSLRSGLIEL-LNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFA 240

Query: 202 IIIPIIKFIQKMLIMMITIINYKFLNLKSGSNYDDGLSLVGVSRVFRQLDLRLKQFNYFP 261
             I +++ I +++++    INY+ + L          +++ +    RQ+DLRL+Q  ++P
Sbjct: 241 YFIILLRIINEVILLA---INYRPIPLS--------YNMMDIFVSARQVDLRLQQACFWP 289

Query: 262 TQFLCYYYDKNILYSRNSLKLPIFNDNLN---INNSNYINLYNSIWLIFNDILIGVTLYH 318
            Q+               +KL +F  +      +   YI  YN++WL+ ND++ G+T+  
Sbjct: 290 VQY---------------MKLWVFRKSKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSS 334

Query: 319 MIMDNFTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDLGKFLGDLYIWTIG 378
            I++N   ++K I   +  +Y    +  ++ W+  + PAG KLN D+ KF+  L +W I 
Sbjct: 335 FILENLHLVVKLIE-NITFEYAIKNVRSMVIWL-VDTPAGLKLNNDICKFIMKLSVWVID 392

Query: 379 FCQNIILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINLITLHIYGFYYCS 438
              N +L          +   + +++  +  +GG S +++ + D ++++T+HI+  Y  S
Sbjct: 393 VWSNFLLHC------LPWTPFLVQVVA-ISGFGGASLMIALISDFLSVMTIHIHLLYLAS 445

Query: 439 AKIYRRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTAIDGFGFDIDQLL 498
           +++Y  QL +I SL QLFRGKK NVLRNRID+                   + +D+DQLL
Sbjct: 446 SRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDS-------------------YEYDLDQLL 486

Query: 499 LGTLLFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIPLFVILLKLKNSK 558
           LGT+LF ++IF LPT++ FY  F +  + ++  L + E +   +   PLFV +L++K+  
Sbjct: 487 LGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPY 546

Query: 559 RLQGGIKFEFIKFY----QGMTILKL--SNKSLTYQEIFINFIKLFR 599
           R+  G+ FE + F      G   L L  ++K ++   +F ++ KL R
Sbjct: 547 RIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLAR 593
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi1p
           [Saccharomyces cerevisiae]
 sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
 gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
          Length = 609

 Score =  499 bits (1273), Expect = e-140
 Identities = 154/516 (29%), Positives = 240/516 (45%), Gaps = 43/516 (8%)

Query: 93  QNQDSYLIYFDPPNFKNLEYFSITPILLQSMGPNLGKSQDDQLFNKLKQFEYQNPLKTKK 152
           +  D   ++F  P  K ++++S  PI L  + P         +   L   + +   + K+
Sbjct: 70  KVADPCNVHFRIPKLKFMQFYSSDPISL--IIPEKEVGLHSSVGETLNYSKLEQHPRYKR 127

Query: 153 DDQSFISDDLVLDKINHLYRNRLLYKRFHSNRSFRALNSKKFNFFIIQNIIIPIIKFIQK 212
           D++  +S+ L +  +   Y   L         S   L     N          I K + K
Sbjct: 128 DNKK-LSETLNIINLFPAYCKALNELYPFIQTSQENLRGTMLNSVAAWCSSTCIYKMVAK 186

Query: 213 MLIMMITIINYKFLNLKSGSNYDDGLSLVGVSRVFRQLDLRLKQFNYFPTQFLCYYYDKN 272
           +   +  +I      + S  NY     LV  S   +Q+DLR +Q  YFP Q+       N
Sbjct: 187 IGFYLTFVICSIASLVSSLLNYSH-FQLVNYSAFVQQIDLRCQQICYFPVQYERINKKDN 245

Query: 273 ILYSRNSLKLPIFNDNLNINNS----------NYINLYNSIWLIFNDILIGVTLYHMIMD 322
           I     ++   +  DN N   S          +YI LYN+IWLI NDI  G+ L  ++++
Sbjct: 246 I----QNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGLILGAILIE 301

Query: 323 NFTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDLGKFLGDLYIWTIGFCQN 382
           N   ++     RV++ +L+ + +K IT     NP G KLN +L  FL +L++W I F   
Sbjct: 302 NRDFLVSASH-RVLKFFLY-DSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSYT 359

Query: 383 IILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINLITLHIYGFYYCSAKIY 442
             +K     +  + +  ++  +  L    G SF +S  ID   +++  IY FY  S+K+Y
Sbjct: 360 TFIKRLIDPKTLSSLLTLTIYMMFLV---GFSFAVSLAIDFFAILSFPIYVFYRISSKLY 416

Query: 443 RRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTAIDGFGFDIDQLLLGTL 502
             QL I+ SLF LF GKK NVLRNRID                      F +DQLLLGTL
Sbjct: 417 HCQLNIMASLFNLFCGKKRNVLRNRID-------------------HNYFQLDQLLLGTL 457

Query: 503 LFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIPLFVILLKLKNSKRLQG 562
           LF++++FL PTV AFY+ + ++ +L +      E +  +I   PLF +LL+LK+ KRL G
Sbjct: 458 LFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPG 517

Query: 563 GIKFEF-IKFYQGMTILKLSNKSLTYQEIFINFIKL 597
           GI  E         T L+L N  + ++ +F  +  L
Sbjct: 518 GISIELKTTVSNKHTTLELQNNPIKFKSMFRPYNLL 553
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
          Length = 517

 Score =  466 bits (1189), Expect = e-130
 Identities = 136/514 (26%), Positives = 252/514 (48%), Gaps = 81/514 (15%)

Query: 110 LEYFSITP--------ILLQSMGPNLGKSQDDQLFNKLKQFEYQNPLKTKKDDQSFISDD 161
           +++ S+ P          +    P     Q  Q+   LK+ +   P + +   +  +   
Sbjct: 1   MQFLSLEPLSLLLLKDSFINKSNPEYESMQHQQIL--LKKLKLHFPRRKENSWKRSLRSG 58

Query: 162 LVLDKINHLYRNRLL------YKRFHSNRSFRALNSKKFNFF-IIQNIIIPIIKFIQKML 214
           L+   +N  +  R+L       K  +  R F  ++S  F FF  +    I +++ I +++
Sbjct: 59  LIEL-LNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFAYFIILLRIINEVI 117

Query: 215 IMMITIINYKFLNLKSGSNYDDGLSLVGVSRVFRQLDLRLKQFNYFPTQFLCYYYDKNIL 274
           ++    INY+ + L          +++ +    RQ+DLRL+Q  ++P Q+          
Sbjct: 118 LLA---INYRPIPLS--------YNMMDIFVSARQVDLRLQQACFWPVQY---------- 156

Query: 275 YSRNSLKLPIFNDNLN---INNSNYINLYNSIWLIFNDILIGVTLYHMIMDNFTSIIKFI 331
                +KL +F  +      +   YI  YN++WL+ ND++ G+T+   I++N   ++K I
Sbjct: 157 -----MKLWVFRKSKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLI 211

Query: 332 RFRVMEKYLFLELMKLITWISTENPAGFKLNTDLGKFLGDLYIWTIGFCQNIILKLDRGS 391
              +  +Y    +  ++ W+  + PAG KLN D+ KF+  L +W I    N +L      
Sbjct: 212 E-NITFEYAIKNVRSMVIWL-VDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHC---- 265

Query: 392 QHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINLITLHIYGFYYCSAKIYRRQLEIIKS 451
               +   + +++  +  +GG S +++ + D ++++T+HI+  Y  S+++Y  QL +I S
Sbjct: 266 --LPWTPFLVQVVA-ISGFGGASLMIALISDFLSVMTIHIHLLYLASSRMYNWQLRVIYS 322

Query: 452 LFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTAIDGFGFDIDQLLLGTLLFMMMIFLL 511
           L QLFRGKK NVLRNRID+                   + +D+DQLLLGT+LF ++IF L
Sbjct: 323 LLQLFRGKKRNVLRNRIDS-------------------YEYDLDQLLLGTILFTVLIFFL 363

Query: 512 PTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIPLFVILLKLKNSKRLQGGIKFEFIKF 571
           PT++ FY  F +  + ++  L + E +   +   PLFV +L++K+  R+  G+ FE + F
Sbjct: 364 PTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEIVSF 423

Query: 572 Y----QGMTILKL--SNKSLTYQEIFINFIKLFR 599
                 G   L L  ++K ++   +F ++ KL R
Sbjct: 424 EPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLAR 457
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
 pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
 emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
          Length = 680

 Score =  446 bits (1136), Expect = e-124
 Identities = 110/564 (19%), Positives = 212/564 (37%), Gaps = 96/564 (17%)

Query: 64  QKTTKQREGGYSNYDLN--------LIYDHTNQLLINQNQDSYLIYFDPPNFKNLEYFSI 115
            K +    G + + +L         +IY     L +N    +Y+I +D P F +  +   
Sbjct: 113 CKYSGLSCGCHKSIELWNSIQVLDCIIYTGMGILYLN-AMSTYVIVYDTPVFGSHHF--- 168

Query: 116 TPILLQSMGPNLGKSQDDQLFNKLKQFEYQNPLKTKKDDQSFISD-DLVLDKINHLYRNR 174
             +   +  P        Q    LK+ ++ + L     ++  +++ + V+  +N      
Sbjct: 169 -SLSFSNSSP--------QTKAPLKKPKWVDDLH----NRKPLNEMETVILSLN---CAA 212

Query: 175 LLYKRFHSNRSFRALNSKKFNFFIIQNIIIPIIKFIQKMLIMMITIINYK---FLNLKSG 231
                +    +   L +   NF I   I     + +  +L  + ++       F  L S 
Sbjct: 213 AAKIAYKKISTQ--LETSSQNFSISYLISSLTWRLLATILGSLSSLYYSLAQFFYLLSSF 270

Query: 232 SNYDDGL-----SLVGVSRVFRQLDLRLKQFNYFPTQFLCYYYDKNILYSRNSLKLPIFN 286
             +          L      FR   +R  Q  Y+P                    +   +
Sbjct: 271 LIFSWVHIASRRVLKNTWINFR---IRSCQILYWPIFLEEID-------------MMSIS 314

Query: 287 DNLNINNSNYINLYNSIWL-IFNDILIGVTLYHMIMDNFTSIIKFIRFRVMEKYLFLELM 345
              +   +      +S W  +  D+++G  +   ++ N  S+  F+ F   +++    L 
Sbjct: 315 CVKHAEEAALQR--HSTWSAMAVDLVLGNLIGLGLLFNTESVCSFV-FDFAKEFTNGILR 371

Query: 346 KLITWISTENPAGFKLNTDLGKFLGDLYIWTIGFCQNIILKLDRGSQHFNYISLMSKILG 405
               W+    PAGFKLNT+L   LG + +  I     + + +       ++I  + +++ 
Sbjct: 372 SGSVWLMGV-PAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMA------SFIFCLIRVIA 424

Query: 406 LLCYYGGCSFLLSFLIDGINLITLHIYGFYYCSAKIYRRQLEIIKSLFQLFRGKKYNVLR 465
           +L    G +   +F+ID I   TLHI   ++    +Y  Q++ + +L++LFRG+K N LR
Sbjct: 425 ILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLR 484

Query: 466 NRIDNLNNYELDNNNNNNTTAIDGFGFDIDQLLLGTLLFMMMIFLLPTVFAFYLVFFIMH 525
            R+D+                   +G+ + Q ++G+LLF  ++ LLPT   FY+ F I  
Sbjct: 485 QRMDS-------------------YGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITS 525

Query: 526 LLILMSLNLLENLQIIICFIPLFVILLKLKNSKRLQGGIKFEFI-----------KFYQG 574
             I     L+E    +I   P   +++ L   KR   G+ FE              F   
Sbjct: 526 TTINSICMLIEFAISVIHATPYAEVMIWLVRRKRFPCGVWFEMEHCGEHILKSNDAFEDS 585

Query: 575 MTILKLSNKSLTYQEIFINFIKLF 598
            ++L+          +  N    F
Sbjct: 586 KSLLEEHGTPEKNSLMVSNLRSNF 609
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
           sapiens]
          Length = 581

 Score =  415 bits (1058), Expect = e-115
 Identities = 97/351 (27%), Positives = 165/351 (46%), Gaps = 41/351 (11%)

Query: 254 LKQFNYFPTQFL--CYYYDKNILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDIL 311
            + F  +P  FL       + + +    L L IF+     N +  +   N++  +  D+ 
Sbjct: 229 CRVFKLWPLSFLGSKLSTCEQLRHRLEHLTL-IFSTRKAENPAQLMRKANTVASVLLDVA 287

Query: 312 IGVTLYHMIM-----DNFTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDLG 366
           +G+ L   +       +    +  +   V E+     L  L+ W+    PAG K+N  L 
Sbjct: 288 LGLMLLSWLHGRSRIGHLADALVPVADHVAEE-----LQHLLQWLMGA-PAGLKMNRALD 341

Query: 367 KFLGDLYIWTIGFCQNIILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINL 426
           + LG  +++ I    + I  +    +H  +   +S  LGL       +  LS L D I L
Sbjct: 342 QVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGL-------TVALSLLSDIIAL 394

Query: 427 ITLHIYGFYYCSAKIYRRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTA 486
           +T HIY FY   A++Y  ++  + SL++LFRGKK+NVLR R+D+ +              
Sbjct: 395 LTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCS-------------- 440

Query: 487 IDGFGFDIDQLLLGTLLFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIP 546
                +D+DQL +GTLLF +++FLLPT   +YLVF ++ LL++    L+  L  +I  +P
Sbjct: 441 -----YDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLP 495

Query: 547 LFVILLKLKNSKRLQGGIKFEFIKFYQGMTI-LKLSNKSLTYQEIFINFIK 596
           L+ + L+L    RL  G+KF  ++   G  + L +    L Y  +   +  
Sbjct: 496 LYSLGLRLCRPYRLAAGVKFRVLRHEAGRPLRLLMQINPLPYSRVVHTYRL 546
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
           component)) [Homo sapiens]
          Length = 583

 Score =  414 bits (1054), Expect = e-114
 Identities = 98/352 (27%), Positives = 166/352 (46%), Gaps = 41/352 (11%)

Query: 253 RLKQFNYFPTQFL--CYYYDKNILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDI 310
           R + F  +P  FL       + + +    L L IF+     N +  +   N++  +  D+
Sbjct: 230 RRRVFKLWPLSFLGSKLSTCEQLRHRLEHLTL-IFSTRKAENPAQLMRKANTVASVLLDV 288

Query: 311 LIGVTLYHMIM-----DNFTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDL 365
            +G+ L   +       +    +  +   V E+     L  L+ W+    PAG K+N  L
Sbjct: 289 ALGLMLLSWLHGRSRIGHLADALVPVADHVAEE-----LQHLLQWLMGA-PAGLKMNRAL 342

Query: 366 GKFLGDLYIWTIGFCQNIILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGIN 425
            + LG  +++ I    + I  +    +H  +   +S  LGL       +  LS L D I 
Sbjct: 343 DQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGL-------TVALSLLSDIIA 395

Query: 426 LITLHIYGFYYCSAKIYRRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTT 485
           L+T HIY FY   A++Y  ++  + SL++LFRGKK+NVLR R+D+ +             
Sbjct: 396 LLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCS------------- 442

Query: 486 AIDGFGFDIDQLLLGTLLFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFI 545
                 +D+DQL +GTLLF +++FLLPT   +YLVF ++ LL++    L+  L  +I  +
Sbjct: 443 ------YDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 496

Query: 546 PLFVILLKLKNSKRLQGGIKFEFIKFYQGMTI-LKLSNKSLTYQEIFINFIK 596
           PL+ + L+L    RL  G+KF  ++   G  + L +    L Y  +   +  
Sbjct: 497 PLYSLGLRLCRPYRLAAGVKFRVLRHEAGRPLRLLMQINPLPYSRVVHTYRL 548
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
           N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
          Length = 581

 Score =  414 bits (1053), Expect = e-114
 Identities = 96/351 (27%), Positives = 164/351 (46%), Gaps = 41/351 (11%)

Query: 254 LKQFNYFPTQFL--CYYYDKNILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDIL 311
            + F  +P  FL       + + +    L L IF+     N +  +   N++  +  D+ 
Sbjct: 229 CRVFKLWPLSFLGSKLSTCEQLRHRLEHLTL-IFSTRKAENPAQLMRKANTVASVLLDVA 287

Query: 312 IGVTLYHMIM-----DNFTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDLG 366
           +G+ L   +       +    +  +   V E+     L  L+ W+    PAG K+N  L 
Sbjct: 288 LGLMLLSWLHGRSRIGHLADALVPVADHVAEE-----LQHLLQWLMGA-PAGLKMNRALD 341

Query: 367 KFLGDLYIWTIGFCQNIILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINL 426
           + LG  +++ I    + I  +    +H  +   +S  LGL       +  LS L D I L
Sbjct: 342 QVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGL-------TVALSLLSDIIAL 394

Query: 427 ITLHIYGFYYCSAKIYRRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTA 486
           +T HIY FY   A++Y  ++  + SL++LFRGKK+NVLR R+D+ +              
Sbjct: 395 LTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCS-------------- 440

Query: 487 IDGFGFDIDQLLLGTLLFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIP 546
                +D+DQL +GTLLF +++FLLPT   +YLVF ++ LL++    L+  L  +I  +P
Sbjct: 441 -----YDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLP 495

Query: 547 LFVILLKLKNSKRLQGGIKFEFIKFYQGMTI-LKLSNKSLTYQEIFINFIK 596
           L+ + L+L    RL  G+KF  ++      + L +    L Y  +   +  
Sbjct: 496 LYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRVVHTYRL 546
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
 gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
           (human) [Mus musculus]
          Length = 581

 Score =  410 bits (1044), Expect = e-113
 Identities = 96/350 (27%), Positives = 163/350 (46%), Gaps = 39/350 (11%)

Query: 254 LKQFNYFPTQFLCYYYDK-NILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDILI 312
            + +  +P  F+         L+ R      IF+     N    +   N +  +  D+ +
Sbjct: 229 CRLWKLWPLSFIRSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVAL 288

Query: 313 GVTLYHMIMDN-----FTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDLGK 367
           G+ L   +  N       + +  +  RV E+     L  L+ W+    PAG K+N  L +
Sbjct: 289 GLLLLSWLHSNNRIGQLANALVPVADRVAEE-----LQHLLQWLMGA-PAGLKMNRALDQ 342

Query: 368 FLGDLYIWTIGFCQNIILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINLI 427
            LG  +++ I    + I  +    +H  +   +S  LGL       +  LS   D I L+
Sbjct: 343 VLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGL-------TVALSIFSDIIALL 395

Query: 428 TLHIYGFYYCSAKIYRRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTAI 487
           T HIY FY   A++Y  ++  + SL++LFRGKK+NVLR R+D+ +               
Sbjct: 396 TFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCS--------------- 440

Query: 488 DGFGFDIDQLLLGTLLFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIPL 547
               +D+DQL +GTLLF +++FLLPT   +YLVF ++ LL++    L+  L  +I  +PL
Sbjct: 441 ----YDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPL 496

Query: 548 FVILLKLKNSKRLQGGIKFEFIKFYQGMTI-LKLSNKSLTYQEIFINFIK 596
           + + L+L    RL  G+KF  ++   G  + L +    L+Y  +   +  
Sbjct: 497 YSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMHIYRL 546
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
           glycosylphosphatidylinositol homolog (S. cerevisiae)
           [Mus musculus]
 dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
          Length = 581

 Score =  407 bits (1036), Expect = e-112
 Identities = 95/350 (27%), Positives = 162/350 (46%), Gaps = 39/350 (11%)

Query: 254 LKQFNYFPTQFLCYYYDK-NILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDILI 312
            + +  +P  F+         L+ R      IF+     N    +   N +  +  D+ +
Sbjct: 229 CRLWKLWPLSFIRSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVAL 288

Query: 313 GVTLYHMIMDN-----FTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDLGK 367
           G+ L   +  N       + +  +  RV E+     L  L+ W+    PAG K+N  L +
Sbjct: 289 GLLLLSWLHSNNRIGQLANALVPVADRVAEE-----LQHLLQWLMGA-PAGLKMNRALDQ 342

Query: 368 FLGDLYIWTIGFCQNIILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINLI 427
            LG  +++ I    + I  +    +H  +   +S  LGL       +  LS   D I L+
Sbjct: 343 VLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGL-------TVALSIFSDIIALL 395

Query: 428 TLHIYGFYYCSAKIYRRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTAI 487
           T HIY FY   A++Y  ++  + SL++LFRGKK+NVLR R+D+ +               
Sbjct: 396 TFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCS--------------- 440

Query: 488 DGFGFDIDQLLLGTLLFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIPL 547
               +D+DQL +GTLLF +++FLLPT   + LVF ++ LL++    L+  L  +I  +PL
Sbjct: 441 ----YDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPL 496

Query: 548 FVILLKLKNSKRLQGGIKFEFIKFYQGMTI-LKLSNKSLTYQEIFINFIK 596
           + + L+L    RL  G+KF  ++   G  + L +    L+Y  +   +  
Sbjct: 497 YSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMHIYRL 546
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
           musculus]
          Length = 581

 Score =  404 bits (1029), Expect = e-111
 Identities = 94/351 (26%), Positives = 162/351 (45%), Gaps = 41/351 (11%)

Query: 254 LKQFNYFPTQFLC--YYYDKNILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDIL 311
            + +   P  F+       + + +    L   IF+     +    +   N +  +  D+ 
Sbjct: 229 CRLWKLGPLAFIRSKLSTCEQLQHRLKHL-SFIFSTEKAQSPMQLMRKANMLVSVLLDVA 287

Query: 312 IGVTLYHMIMDN-----FTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDLG 366
           +G+ L   +  N       + +  +  RV E+     L  L+ W+    PAG K+N  L 
Sbjct: 288 LGLLLLSWLHSNNRIGQLANALVPVADRVAEE-----LQHLLQWLMGA-PAGLKMNRALD 341

Query: 367 KFLGDLYIWTIGFCQNIILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINL 426
           + LG  +++ I    + I  +    +H  +   +S  LGL       +  LS   D I L
Sbjct: 342 QVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGL-------TVALSIFSDIIAL 394

Query: 427 ITLHIYGFYYCSAKIYRRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTA 486
           +T HIY FY   A++Y  ++  + SL++LFRGKK+NVLR R+D+ +              
Sbjct: 395 LTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCS-------------- 440

Query: 487 IDGFGFDIDQLLLGTLLFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIP 546
                +D+DQL +GTLLF +++FLLPT   +YLVF ++ LL++    L+  L  +I  +P
Sbjct: 441 -----YDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLP 495

Query: 547 LFVILLKLKNSKRLQGGIKFEFIKFYQGMTI-LKLSNKSLTYQEIFINFIK 596
           L+ + L+L    RL  G+KF  ++   G  + L +    L Y  +   +  
Sbjct: 496 LYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLPYSHVVHTYRL 546
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
 gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 760

 Score =  386 bits (983), Expect = e-106
 Identities = 90/315 (28%), Positives = 151/315 (47%), Gaps = 40/315 (12%)

Query: 254 LKQFNYFPTQFL--CYYYDKNILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDIL 311
            + F  +P  FL       + + +    L L IF+     N +  +   N++  +  D+ 
Sbjct: 229 CRVFKLWPLSFLGSKLSTCEQLRHRLEHLTL-IFSTRKAENPAQLMRKANTVASVLLDVA 287

Query: 312 IGVTLYHMIM-----DNFTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDLG 366
           +G+ L   +       +    +  +   V E+     L  L+ W+    PAG K+N  L 
Sbjct: 288 LGLMLLSWLHGRSRIGHLADALVPVADHVAEE-----LQHLLQWLMGA-PAGLKMNRALD 341

Query: 367 KFLGDLYIWTIGFCQNIILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINL 426
           + LG  +++ I    + I  +    +H  +   +S  LGL       +  LS L D I L
Sbjct: 342 QVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGL-------TVALSLLSDIIAL 394

Query: 427 ITLHIYGFYYCSAKIYRRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTA 486
           +T HIY FY   A++Y  ++  + SL++LFRGKK+NVLR R+D+ +              
Sbjct: 395 LTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCS-------------- 440

Query: 487 IDGFGFDIDQLLLGTLLFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIP 546
                +D+DQL +GTLLF +++FLLPT   +YLVF ++ LL++    L+  L  +I  +P
Sbjct: 441 -----YDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLP 495

Query: 547 LFVILLKLKNSKRLQ 561
           L+ + L+L    RL 
Sbjct: 496 LYSLGLRLCRPYRLA 510
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
          Length = 339

 Score =  369 bits (940), Expect = e-101
 Identities = 94/337 (27%), Positives = 158/337 (45%), Gaps = 39/337 (11%)

Query: 266 CYYYDKNILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDILIGVTLYHMIMDN-- 323
                +  L+ R      IF+     N    +   N +  +  D+ +G+ L   +  N  
Sbjct: 1   KLSTCEQ-LHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNR 59

Query: 324 ---FTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDLGKFLGDLYIWTIGFC 380
                + +  +  RV E+     L  L+ W+    PAG K+N  L + LG  +++ I   
Sbjct: 60  IGQLANALVPVADRVAEE-----LQHLLQWLMGA-PAGLKMNRALDQVLGRFFLYHIHLW 113

Query: 381 QNIILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINLITLHIYGFYYCSAK 440
            + I  +    +H  +   +S  LGL       +  LS   D I L+T HIY FY   A+
Sbjct: 114 ISYIHLMSPFIEHILWHVGLSACLGL-------TVALSIFSDIIALLTFHIYCFYVYGAR 166

Query: 441 IYRRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTAIDGFGFDIDQLLLG 500
           +Y  ++  + SL++LFRGKK+NVLR R+D+ +                   +D+DQL +G
Sbjct: 167 LYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCS-------------------YDLDQLFIG 207

Query: 501 TLLFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIPLFVILLKLKNSKRL 560
           TLLF +++FLLPT   +YLVF ++ LL++    L+  L  +I  +PL+ + L+L    RL
Sbjct: 208 TLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRL 267

Query: 561 QGGIKFEFIKFYQGMTI-LKLSNKSLTYQEIFINFIK 596
             G+KF  ++   G  + L +    L+Y  +   +  
Sbjct: 268 AAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMHIYRL 304
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
          Length = 669

 Score =  309 bits (785), Expect = 9e-83
 Identities = 103/477 (21%), Positives = 184/477 (37%), Gaps = 68/477 (14%)

Query: 111 EYFSITPILLQSMGPNLGKSQDDQLFNKL-KQFEYQNPLKTKKDDQSFISDDLVLDKINH 169
             +S       S   N    ++D    +L K  + +       D   +IS + + DK+  
Sbjct: 166 HIYSENITPSSSNNNNDNNKENDCDKEQLDKYNKDKENKLKLNDKDEYISFNFIEDKLTE 225

Query: 170 LY------------RNRLLYKRFHSNRSF------RALNSKKFNFFIIQNIIIPIIKFIQ 211
            +                     + NR F           KK     I + I+ II F  
Sbjct: 226 SFHMNQIIHLINKKCVFTKCLENYKNRYFVLKKEEILKKKKKQKKMSIFSYIVSIILFFT 285

Query: 212 KMLIMMITIINYKFLNLKSGSNYDDGLSLVGVSRVFRQLDLRLKQFNYFPTQFLCYYYDK 271
            ++ ++ + + Y     K  S Y             ++ D  L+   Y   +F+      
Sbjct: 286 YIISLINSCLYYIICTPKLFSEY----------IFSKKCDGYLQNSAY--PKFIFPSEWH 333

Query: 272 NILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDILIGVTLYHMIMDNFTSIIKFI 331
           NI  S        F  N   N S Y      + +   +++I + L  +I       +  +
Sbjct: 334 NIFRS--------FMKNK-QNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINL 384

Query: 332 RFRV--MEKYLFLELMKLITWISTENPAGFKLNTDLGKFLGDLYIWTIGFCQNIILKLDR 389
            +     + +     +  I     +NP GFKLN +   F+G + +  +       L  + 
Sbjct: 385 HYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWD---LFTNT 441

Query: 390 GSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINLITLHIYGFYYCSAKIYRRQLEII 449
              + + +        LL    G SF LSF+ID +  +T H+   Y    KI     + +
Sbjct: 442 IPVNNSTVLNFVGYTSLL----GFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNM 497

Query: 450 KSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTAIDGFGFDIDQLLLGTLLFMMMIF 509
            SL+ LF GKK+N+L+ R+D                      +  +++LLGT+LF ++IF
Sbjct: 498 YSLYLLFNGKKWNILKLRVDT-------------------NYYSNEEVLLGTILFTILIF 538

Query: 510 LLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIPLFVILLKLKNSKRLQGGIKF 566
           L PT+F   LVF +++L+I   + LL  ++ II + P ++  ++   +K +  G KF
Sbjct: 539 LYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGFKF 595
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
          Length = 481

 Score =  251 bits (636), Expect = 3e-65
 Identities = 59/279 (21%), Positives = 117/279 (41%), Gaps = 39/279 (13%)

Query: 279 SLKLPIFNDNLNINNSNYINLYNSIWLIFNDILIGVTLYHMIMDNFTSIIKFIRFRVMEK 338
             K      +            N +      I++ + L+ +       +I+   + + E 
Sbjct: 205 HFKEWQTACDKRS------RPANIVLDRILGIILMLILFSLATQPGDFLIQISHYVIDEL 258

Query: 339 YLFLELMKLITWISTENPAGFKLNTDLGKFLGDLYIWTIGFCQNIILKLDRGSQHFNYIS 398
           Y  L++++        +P G KLN  L  F  D + + I      +   D        + 
Sbjct: 259 YGLLKVLE-------GSPIGLKLNIHLNNFFLDCFKYHIELWSTFL---DFIEPLVRQVF 308

Query: 399 LMSKILGLLCYYGGCSFLLSFLIDGINLITLHIYGFYYCSAKIYRRQLEIIKSLFQLFRG 458
           L   ++G L    G +F ++ L+D I++I LH + FY  +  +Y  +   +  L+Q+ RG
Sbjct: 309 LAIGMIGCL----GFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRG 364

Query: 459 KKYNVLRNRIDNLNNYELDNNNNNNTTAIDGFGFDIDQLLLGTLLFMMMIFLLPTVFAFY 518
            +YN+L+ R ++ N                   +   QL L T+ F  ++FLLPT   +Y
Sbjct: 365 NRYNILKGRTESHN-------------------YMNRQLYLATIFFSAILFLLPTTLVYY 405

Query: 519 LVFFIMHLLILMSLNLLENLQIIICFIPLFVILLKLKNS 557
           +VF  +  L   +L++   ++  + ++P+ V + +L   
Sbjct: 406 IVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRLLRG 444
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
          Length = 473

 Score =  238 bits (602), Expect = 3e-61
 Identities = 62/297 (20%), Positives = 118/297 (38%), Gaps = 41/297 (13%)

Query: 263 QFLCYYYDKNILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDILIGVTLYHMIMD 322
           +F+       ++Y     K                 L N +      I++ + L+ ++  
Sbjct: 184 KFINSIITHTVIYR--HYKEWQSIYTKGS------RLSNIMIDRALGIILMLVLFTLVSH 235

Query: 323 NFTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDLGKFLGDLYIWTIGFCQN 382
               +I+     + + Y  L++++        +P G KLN  L  F  D + + I     
Sbjct: 236 PGDFLIQISHIIIHQLYSLLKVLE-------GSPIGLKLNIHLNNFFLDCFKYHIELWST 288

Query: 383 IILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGINLITLHIYGFYYCSAKIY 442
            +   D        + L     G L    G ++ ++ L D I+++ LH + FY  +  + 
Sbjct: 289 FL---DLIEPIVRQVFLAIGAFGCL----GFTYQIALLADLISIVGLHAHCFYVYTKVLN 341

Query: 443 RRQLEIIKSLFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTAIDGFGFDIDQLLLGTL 502
             +++ +  L+Q+ RG +YN+LRNRI+  N                   +   QL L T+
Sbjct: 342 NVEVKGLTVLWQVVRGNRYNILRNRIEAHN-------------------YMNRQLYLATI 382

Query: 503 LFMMMIFLLPTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIPLFVILLKLKNSKR 559
            F  ++FL PT   +Y+VF  +  L   +L  LE  +  +   P+ + L  +K    
Sbjct: 383 FFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIKKGFY 439
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
 pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
 emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
          Length = 287

 Score =  211 bits (533), Expect = 3e-53
 Identities = 56/231 (24%), Positives = 93/231 (40%), Gaps = 35/231 (15%)

Query: 343 ELMKLITWISTENPAGFKLNTDLGKFLGDLYIWTIGFCQNIILKLDRGSQHFNYISLMSK 402
            L   ITW+ + NPAG KLNT + + L   + + I    +++    + +  FN+ +  S 
Sbjct: 65  NLNGTITWLRS-NPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFA--FNFHNDFSA 121

Query: 403 ILGLLCYYGGCSFLLSFLIDGINLITLHIY-----------GFYYCSAKIYRRQLEIIKS 451
            +G L       F+   LI GI+  +  +Y            F   + K+       +  
Sbjct: 122 FIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTV 181

Query: 452 LFQLFRGKKYNVLRNRIDNLNNYELDNNNNNNTTAIDGFGFDIDQLLLGTLLFMMMIFLL 511
           L+ L RGKK+N LR R D +                     D  Q  L T LF++++F+L
Sbjct: 182 LWSLVRGKKWNPLRERKDTVI-------------------LDTRQQFLATSLFVILLFIL 222

Query: 512 PTVFAFYLVFFIMHLLILMSLNLLENLQIIICFIPLFVILLKLKNSKRLQG 562
           PT+F +++VF  + L +     +L           LF +   L        
Sbjct: 223 PTIFVYFVVFRCLRLAVSALQTVLYFFATW--PFQLFALEKHLAEKYGKPA 271
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana  (fragment)
 emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
          Length = 143

 Score =  149 bits (374), Expect = 1e-34
 Identities = 36/151 (23%), Positives = 74/151 (48%), Gaps = 8/151 (5%)

Query: 306 IFNDILIGVTLYHMIMDNFTSIIKFIRFRVMEKYLFLELMKLITWISTENPAGFKLNTDL 365
           +  D+++G  +   ++ N  S+  F+ F   +++    L     W+    PAGFKLNT+L
Sbjct: 1   MAVDLVLGNLIGLGLLFNTESVCSFV-FDFAKEFTNGILRSGSVWLMGV-PAGFKLNTEL 58

Query: 366 GKFLGDLYIWTIGFCQNIILKLDRGSQHFNYISLMSKILGLLCYYGGCSFLLSFLIDGIN 425
              LG + +  I     + + +       ++I  + +++ +L    G +   +F+ID I 
Sbjct: 59  AGVLGMVSLNVIQIWSTLWVFMA------SFIFCLIRVIAILGITFGATVSAAFVIDVIT 112

Query: 426 LITLHIYGFYYCSAKIYRRQLEIIKSLFQLF 456
             TLHI   ++    +Y  Q++ + +L++LF
Sbjct: 113 FATLHIMALHWAITLVYSHQIQALAALWRLF 143
>emb|CAB68123.2| (AL137080) hypothetical protein [Arabidopsis thaliana]
          Length = 261

 Score = 81.0 bits (198), Expect = 6e-14
 Identities = 16/107 (14%), Positives = 38/107 (34%), Gaps = 22/107 (20%)

Query: 96  DSYLIYFDPPNFKNLEYFSITPILLQSMGPNLGKSQDDQLFNKLKQFEYQNPLKTKKDDQ 155
           D ++I +D P F +  +     +   +  P        Q    LK+ ++ + L     ++
Sbjct: 176 DVHVIVYDTPVFGSHHF----SLSFSNSSP--------QTKAPLKKPKWVDDLH----NR 219

Query: 156 SFISD-DLVLDKINHLYRNRLLYKRFHSNRSFRALNSKKFNFFIIQN 201
             +++ + V+  +N           +    +   L +   NF I   
Sbjct: 220 KPLNEMETVILSLN---CAAAAKIAYKKISTQ--LETSSQNFSISYL 261
>dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:1333114,
           evidence:ISS~phosphatidylinositol glycan, class
           Q~putative [Mus musculus]
          Length = 378

 Score = 78.6 bits (192), Expect = 3e-13
 Identities = 12/86 (13%), Positives = 28/86 (31%), Gaps = 6/86 (6%)

Query: 254 LKQFNYFPTQFLCYYYDK-NILYSRNSLKLPIFNDNLNINNSNYINLYNSIWLIFNDILI 312
            + +  +P  F+         L+ R      IF+     N    +   N +  +  D+ +
Sbjct: 229 CRLWKLWPLSFIRSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVAL 288

Query: 313 GVTLYHMIMDN-----FTSIIKFIRF 333
           G+ L   +  N       + +  +  
Sbjct: 289 GLLLLSWLHSNNRIGQLANALVPVAD 314
>pir||T45795 hypothetical protein F28O9.20 - Arabidopsis thaliana
          Length = 242

 Score = 74.3 bits (181), Expect = 5e-12
 Identities = 12/83 (14%), Positives = 31/83 (36%), Gaps = 20/83 (24%)

Query: 96  DSYLIYFDPPNFKNLEYFSITPILLQSMGPNLGKSQDDQLFNKLKQFEYQNPLKTKKDDQ 155
           D ++I +D P F +  +     +   +  P        Q    LK+ ++ + L     ++
Sbjct: 176 DVHVIVYDTPVFGSHHF----SLSFSNSSP--------QTKAPLKKPKWVDDLH----NR 219

Query: 156 SFISD-DLVLDKINHLYRNRLLY 177
             +++ + V+  +N         
Sbjct: 220 KPLNEMETVILSLN---CAAAAK 239
>gb|AAH10094.1|AAH10094 (BC010094) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 299

 Score = 63.4 bits (153), Expect = 1e-08
 Identities = 9/47 (19%), Positives = 17/47 (36%), Gaps = 3/47 (6%)

Query: 254 LKQFNYFPTQFL--CYYYDKNILYSRNSLKLPIFNDNLNINNSNYIN 298
            + F  +P  FL       + + +    L L IF+     N +  + 
Sbjct: 229 CRVFKLWPLSFLGSKLSTCEQLRHRLEHLTL-IFSTRKAENPAQLMR 274
CPU time:   163.58 user secs.	    2.63 sys. secs	  166.21 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.319    0.167    0.479 

Gapped
Lambda     K      H
   0.270   0.0584    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 382332669
Number of Sequences: 887402
Number of extensions: 17483590
Number of successful extensions: 90445
Number of sequences better than 10.0: 438
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 421
Number of HSP's that attempted gapping in prelim test: 89474
Number of HSP's gapped (non-prelim): 1093
length of query: 648
length of database: 277,845,442
effective HSP length: 54
effective length of query: 594
effective length of database: 229,925,734
effective search space: 136575885996
effective search space used: 136575885996
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.0 bits)
S2: 76 (33.7 bits)