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GPI Anchor Biosynthesis Report: T20486 (PIG-Q family, Caenorhabditis elegans)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (287 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 18 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 355 6e-97
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 353 3e-96
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 351 6e-96
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 351 7e-96
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 351 9e-96
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 339 4e-92
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 338 6e-92
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 335 4e-91
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 318 7e-86
sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 286 4e-76
emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 277 2e-73
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 276 4e-73
emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 258 1e-67
ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 253 3e-66
ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 233 2e-60
emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 197 3e-49
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 130 3e-29
Sequences with E-value WORSE than threshold

dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:13331... 38 0.19
Alignments
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
 pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
 emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
          Length = 287

 Score =  355 bits (903), Expect = 6e-97
 Identities = 287/287 (100%), Positives = 287/287 (100%)

Query: 1   MDKITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTG 60
           MDKITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTG
Sbjct: 1   MDKITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTG 60

Query: 61  NVADNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFS 120
           NVADNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFS
Sbjct: 61  NVADNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFS 120

Query: 121 AFIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLT 180
           AFIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLT
Sbjct: 121 AFIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLT 180

Query: 181 VLWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVS 240
           VLWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVS
Sbjct: 181 VLWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVS 240

Query: 241 ALQTVLYFFATWPFQLFALEKHLAEKYGKPADAQNEALAEKKTKSQN 287
           ALQTVLYFFATWPFQLFALEKHLAEKYGKPADAQNEALAEKKTKSQN
Sbjct: 241 ALQTVLYFFATWPFQLFALEKHLAEKYGKPADAQNEALAEKKTKSQN 287
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
           N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
          Length = 581

 Score =  353 bits (897), Expect = 3e-96
 Identities = 84/279 (30%), Positives = 128/279 (45%), Gaps = 24/279 (8%)

Query: 12  RTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWT--PNWEWTVNEFWDQTGNVADNLNGT 69
           R  E    L+R  +    +LLDVA  + L +            +       +VA+ L   
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323

Query: 70  ITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLR 127
           + WL   PAGLK+N  +++ L  FF YHI+LW S +     F  +  +H   SA +    
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACL---- 379

Query: 128 SDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVR 187
                         G++   +++ D   +   H  CF  Y  +L  L  + L+ LW L R
Sbjct: 380 --------------GLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFR 425

Query: 188 GKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLY 247
           GKKWN LR+R D+   D  Q F+ T LF ILLF+LPT  +Y++VF  LRL V A+Q +++
Sbjct: 426 GKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIH 485

Query: 248 FFATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
                     L++L   L   Y   A  +   L  + ++
Sbjct: 486 LLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASR 524
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
 gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 760

 Score =  351 bits (894), Expect = 6e-96
 Identities = 82/265 (30%), Positives = 122/265 (45%), Gaps = 24/265 (9%)

Query: 12  RTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWT--PNWEWTVNEFWDQTGNVADNLNGT 69
           R  E    L+R  +    +LLDVA  + L +            +       +VA+ L   
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323

Query: 70  ITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLR 127
           + WL   PAGLK+N  +++ L  FF YHI+LW S +     F  +  +H   SA +    
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACL---- 379

Query: 128 SDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVR 187
                         G++   +++ D   +   H  CF  Y  +L  L  + L+ LW L R
Sbjct: 380 --------------GLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFR 425

Query: 188 GKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLY 247
           GKKWN LR+R D+   D  Q F+ T LF ILLF+LPT  +Y++VF  LRL V A+Q +++
Sbjct: 426 GKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIH 485

Query: 248 FFATW--PFQLFALEKHLAEKYGKP 270
                     L++L   L   Y   
Sbjct: 486 LLVDLINSLPLYSLGLRLCRPYRLA 510
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
           component)) [Homo sapiens]
          Length = 583

 Score =  351 bits (894), Expect = 7e-96
 Identities = 84/279 (30%), Positives = 127/279 (45%), Gaps = 24/279 (8%)

Query: 12  RTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWT--PNWEWTVNEFWDQTGNVADNLNGT 69
           R  E    L+R  +    +LLDVA  + L +            +       +VA+ L   
Sbjct: 266 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 325

Query: 70  ITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLR 127
           + WL   PAGLK+N  +++ L  FF YHI+LW S +     F  +  +H   SA +    
Sbjct: 326 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACL---- 381

Query: 128 SDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVR 187
                         G++   +++ D   +   H  CF  Y  +L  L  + L+ LW L R
Sbjct: 382 --------------GLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFR 427

Query: 188 GKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLY 247
           GKKWN LR+R D+   D  Q F+ T LF ILLF+LPT  +Y++VF  LRL V A+Q +++
Sbjct: 428 GKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIH 487

Query: 248 FFATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
                     L++L   L   Y   A  +   L  +  +
Sbjct: 488 LLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEAGR 526
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
           sapiens]
          Length = 581

 Score =  351 bits (893), Expect = 9e-96
 Identities = 84/279 (30%), Positives = 127/279 (45%), Gaps = 24/279 (8%)

Query: 12  RTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWT--PNWEWTVNEFWDQTGNVADNLNGT 69
           R  E    L+R  +    +LLDVA  + L +            +       +VA+ L   
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323

Query: 70  ITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLR 127
           + WL   PAGLK+N  +++ L  FF YHI+LW S +     F  +  +H   SA +    
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACL---- 379

Query: 128 SDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVR 187
                         G++   +++ D   +   H  CF  Y  +L  L  + L+ LW L R
Sbjct: 380 --------------GLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFR 425

Query: 188 GKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLY 247
           GKKWN LR+R D+   D  Q F+ T LF ILLF+LPT  +Y++VF  LRL V A+Q +++
Sbjct: 426 GKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIH 485

Query: 248 FFATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
                     L++L   L   Y   A  +   L  +  +
Sbjct: 486 LLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEAGR 524
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
 gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
           (human) [Mus musculus]
          Length = 581

 Score =  339 bits (862), Expect = 4e-92
 Identities = 83/278 (29%), Positives = 126/278 (44%), Gaps = 24/278 (8%)

Query: 13  TLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTGNVADNLNGTI 70
             +  + L+R  +    +LLDVA  + L +    N       N        VA+ L   +
Sbjct: 265 KAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLL 324

Query: 71  TWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLRS 128
            WL   PAGLK+N  +++ L  FF YHI+LW S +     F  +  +H   SA +     
Sbjct: 325 QWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACL----- 379

Query: 129 DAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRG 188
                        G++   ++  D   +   H  CF  Y  +L  L  Y L+ LW L RG
Sbjct: 380 -------------GLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRG 426

Query: 189 KKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYF 248
           KKWN LR+R D+   D  Q F+ T LF IL+F+LPT  +Y++VF  LRL V  +Q +++ 
Sbjct: 427 KKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHL 486

Query: 249 FATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
                    L++L   L   Y   A  +   L ++  +
Sbjct: 487 LVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGR 524
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
           musculus]
          Length = 581

 Score =  338 bits (860), Expect = 6e-92
 Identities = 83/278 (29%), Positives = 127/278 (44%), Gaps = 24/278 (8%)

Query: 13  TLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTGNVADNLNGTI 70
             ++ + L+R  +    +LLDVA  + L +    N       N        VA+ L   +
Sbjct: 265 KAQSPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLL 324

Query: 71  TWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLRS 128
            WL   PAGLK+N  +++ L  FF YHI+LW S +     F  +  +H   SA +     
Sbjct: 325 QWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACL----- 379

Query: 129 DAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRG 188
                        G++   ++  D   +   H  CF  Y  +L  L  Y L+ LW L RG
Sbjct: 380 -------------GLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRG 426

Query: 189 KKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYF 248
           KKWN LR+R D+   D  Q F+ T LF IL+F+LPT  +Y++VF  LRL V  +Q +++ 
Sbjct: 427 KKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHL 486

Query: 249 FATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
                    L++L   L   Y   A  +   L ++  +
Sbjct: 487 LVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGR 524
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
           glycosylphosphatidylinositol homolog (S. cerevisiae)
           [Mus musculus]
 dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
          Length = 581

 Score =  335 bits (853), Expect = 4e-91
 Identities = 83/278 (29%), Positives = 125/278 (44%), Gaps = 24/278 (8%)

Query: 13  TLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTGNVADNLNGTI 70
             +  + L+R  +    +LLDVA  + L +    N       N        VA+ L   +
Sbjct: 265 KAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLL 324

Query: 71  TWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLRS 128
            WL   PAGLK+N  +++ L  FF YHI+LW S +     F  +  +H   SA +     
Sbjct: 325 QWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACL----- 379

Query: 129 DAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRG 188
                        G++   ++  D   +   H  CF  Y  +L  L  Y L+ LW L RG
Sbjct: 380 -------------GLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRG 426

Query: 189 KKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYF 248
           KKWN LR+R D+   D  Q F+ T LF IL+F+LPT  +Y +VF  LRL V  +Q +++ 
Sbjct: 427 KKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHL 486

Query: 249 FATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
                    L++L   L   Y   A  +   L ++  +
Sbjct: 487 LVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGR 524
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
          Length = 339

 Score =  318 bits (808), Expect = 7e-86
 Identities = 83/278 (29%), Positives = 126/278 (44%), Gaps = 24/278 (8%)

Query: 13  TLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTGNVADNLNGTI 70
             +  + L+R  +    +LLDVA  + L +    N       N        VA+ L   +
Sbjct: 23  KAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLL 82

Query: 71  TWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLRS 128
            WL   PAGLK+N  +++ L  FF YHI+LW S +     F  +  +H   SA +     
Sbjct: 83  QWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACL----- 137

Query: 129 DAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRG 188
                        G++   ++  D   +   H  CF  Y  +L  L  Y L+ LW L RG
Sbjct: 138 -------------GLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRG 184

Query: 189 KKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYF 248
           KKWN LR+R D+   D  Q F+ T LF IL+F+LPT  +Y++VF  LRL V  +Q +++ 
Sbjct: 185 KKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHL 244

Query: 249 FATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
                    L++L   L   Y   A  +   L ++  +
Sbjct: 245 LVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGR 282
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
           - fission yeast (Schizosaccharomyces pombe)
 emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
           protein gpi1. [Schizosaccharomyces pombe]
          Length = 653

 Score =  286 bits (725), Expect = 4e-76
 Identities = 71/270 (26%), Positives = 117/270 (43%), Gaps = 25/270 (9%)

Query: 21  VRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVA-DNLNGTITWLRSNPAG 79
           +R ++   L+  D+ F I + +    N    V    + T   A  N+   + WL   PAG
Sbjct: 313 IRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAG 372

Query: 80  LKLNTPVNETLAWFFTYHIYLWTSM-LFCFQKFAFNFHNDFSAFIGFLRSDAFFRFIAYS 138
           LKLN  + + +     + I +W++  L C     F                   + +A S
Sbjct: 373 LKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPF-----------------LVQVVAIS 415

Query: 139 LIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLRERK 198
             GG S   A++ DF  +  +H +     +++L       +  L  L RGKK N LR R 
Sbjct: 416 GFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRI 475

Query: 199 DTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAV----SALQTVLYFFATWPF 254
           D+   D  Q  L T LF +L+F LPTI+V++  F   R++V    +  +T+L F   +P 
Sbjct: 476 DSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFP- 534

Query: 255 QLFALEKHLAEKYGKPADAQNEALAEKKTK 284
            LF     + + Y  P+    E ++ +  K
Sbjct: 535 -LFVTMLRIKDPYRIPSGLNFEIVSFEPLK 563
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
          Length = 473

 Score =  277 bits (702), Expect = 2e-73
 Identities = 70/261 (26%), Positives = 108/261 (40%), Gaps = 20/261 (7%)

Query: 1   MDKITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTG 60
           ++ I  + V+ R  +    +    S    +++D A  I L  +         +     + 
Sbjct: 186 INSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFLIQISH 245

Query: 61  NVADNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFS 120
            +   L   +  L  +P GLKLN  +N      F YHI LW++ L               
Sbjct: 246 IIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFL--------------- 290

Query: 121 AFIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLT 180
             I  +    F    A+  + G +   A++ D   I  LH +CF  Y   L  +    LT
Sbjct: 291 DLIEPIVRQVFLAIGAFGCL-GFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLT 349

Query: 181 VLWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVS 240
           VLW +VRG ++N LR R +      RQ +LAT  F  +LF+ PT  VY+VVF  L+    
Sbjct: 350 VLWQVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTC 409

Query: 241 ALQTVLYFFA----TWPFQLF 257
           A    L  F      +P ++F
Sbjct: 410 ATLATLECFRRKLLNFPIEMF 430
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
          Length = 517

 Score =  276 bits (700), Expect = 4e-73
 Identities = 70/270 (25%), Positives = 117/270 (42%), Gaps = 25/270 (9%)

Query: 21  VRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVA-DNLNGTITWLRSNPAG 79
           +R ++   L+  D+ F I + +    N    V    + T   A  N+   + WL   PAG
Sbjct: 177 IRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAG 236

Query: 80  LKLNTPVNETLAWFFTYHIYLWTSM-LFCFQKFAFNFHNDFSAFIGFLRSDAFFRFIAYS 138
           LKLN  + + +     + I +W++  L C     F                   + +A S
Sbjct: 237 LKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPF-----------------LVQVVAIS 279

Query: 139 LIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLRERK 198
             GG S   A++ DF  +  +H +     ++++       +  L  L RGKK N LR R 
Sbjct: 280 GFGGASLMIALISDFLSVMTIHIHLLYLASSRMYNWQLRVIYSLLQLFRGKKRNVLRNRI 339

Query: 199 DTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAV----SALQTVLYFFATWPF 254
           D+   D  Q  L T LF +L+F LPTI+V++  F   R++V    +  +T+L F   +P 
Sbjct: 340 DSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFP- 398

Query: 255 QLFALEKHLAEKYGKPADAQNEALAEKKTK 284
            LF     + + Y  P+    E ++ +  K
Sbjct: 399 -LFVTMLRIKDPYRIPSGLNFEIVSFEPLK 427
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
          Length = 481

 Score =  258 bits (653), Expect = 1e-67
 Identities = 68/250 (27%), Positives = 105/250 (41%), Gaps = 16/250 (6%)

Query: 1   MDKITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTG 60
           ++ I  +  + +  +         S    ++LD    I L  I         +     + 
Sbjct: 193 INSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLATQPGDFLIQISH 252

Query: 61  NVADNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFS 120
            V D L G +  L  +P GLKLN  +N      F YHI LW++ L               
Sbjct: 253 YVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFL--------------- 297

Query: 121 AFIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLT 180
            FI  L    F        + G +   A++ D   +  LH +CF  Y   L  +    L+
Sbjct: 298 DFIEPLVRQVFLAIGMIGCL-GFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLS 356

Query: 181 VLWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVS 240
           VLW +VRG ++N L+ R ++     RQ +LAT  F  +LF+LPT  VY++VF  L+    
Sbjct: 357 VLWQVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTF 416

Query: 241 ALQTVLYFFA 250
           A  +V +F  
Sbjct: 417 ATLSVFHFVR 426
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi1p
           [Saccharomyces cerevisiae]
 sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
 gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
          Length = 609

 Score =  253 bits (642), Expect = 3e-66
 Identities = 61/259 (23%), Positives = 105/259 (39%), Gaps = 21/259 (8%)

Query: 24  FSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQ-TGNVADNLNGTITWLRSNPAGLKL 82
           ++   L++ D++F + L  I   N ++ V+         + D+L      L +NP G+KL
Sbjct: 279 YNTIWLIINDISFGLILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKL 338

Query: 83  NTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGFLRSDAFFRFIAYSL-IG 141
           N  +   L+  F + I              F++       I      +      Y + + 
Sbjct: 339 NAELANFLSELFLWVIE-------------FSYTTFIKRLIDPKTLSSLLTLTIYMMFLV 385

Query: 142 GISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLRERKDTV 201
           G S   ++  DF  I       F   ++KL +     +  L++L  GKK N LR R D  
Sbjct: 386 GFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHN 445

Query: 202 ILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSAL----QTVLYFFATWPFQLF 257
                Q  L T LF+IL+F+ PT+  +++ +  LR+    +    + V+     +P  LF
Sbjct: 446 YFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFP--LF 503

Query: 258 ALEKHLAEKYGKPADAQNE 276
           AL   L +    P     E
Sbjct: 504 ALLLRLKDPKRLPGGISIE 522
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
 pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
 emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
          Length = 680

 Score =  233 bits (591), Expect = 2e-60
 Identities = 59/274 (21%), Positives = 99/274 (35%), Gaps = 22/274 (8%)

Query: 7   NVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVADN- 65
           ++  ++  E   L  +  S    + +D+     +      N E   +  +D      +  
Sbjct: 312 SISCVKHAEEAAL--QRHSTWSAMAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGI 369

Query: 66  LNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGF 125
           L     WL   PAG KLNT +   L       I +W+++      F F            
Sbjct: 370 LRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIF------------ 417

Query: 126 LRSDAFFRFIAY-SLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWS 184
                  R IA   +  G +  +A V D      LH        T +       L  LW 
Sbjct: 418 ----CLIRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWR 473

Query: 185 LVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQT 244
           L RG+K NPLR+R D+     +Q  + + LF  LL +LPT  V+++ F      ++++  
Sbjct: 474 LFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICM 533

Query: 245 VLYFFATW--PFQLFALEKHLAEKYGKPADAQNE 276
           ++ F  +         +   L  +   P     E
Sbjct: 534 LIEFAISVIHATPYAEVMIWLVRRKRFPCGVWFE 567
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
          Length = 669

 Score =  197 bits (497), Expect = 3e-49
 Identities = 53/236 (22%), Positives = 88/236 (36%), Gaps = 20/236 (8%)

Query: 11  IRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVADNLNGTI 70
                  +L+        + L  + F +  +N+   N  +   +   Q       L   +
Sbjct: 349 YYKYREILLIRIINLIIDIFLGFLIFLLLYFNVI--NLHYISEK--AQIFYGTSTLTSIL 404

Query: 71  TWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGFLRSDA 130
             L  NP G KLN      +       +  W                        + +  
Sbjct: 405 GTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTI----------------PVNNST 448

Query: 131 FFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKK 190
              F+ Y+ + G S F + V D+ +    H      +  K+C L +  +  L+ L  GKK
Sbjct: 449 VLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKK 508

Query: 191 WNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVL 246
           WN L+ R DT      +  L T LF IL+F+ PTIFV  +VF  + L ++ +  +L
Sbjct: 509 WNILKLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLL 564
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana  (fragment)
 emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
          Length = 143

 Score =  130 bits (326), Expect = 3e-29
 Identities = 33/159 (20%), Positives = 49/159 (30%), Gaps = 18/159 (11%)

Query: 30  LLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVADN-LNGTITWLRSNPAGLKLNTPVNE 88
           + +D+     +      N E   +  +D      +  L     WL   PAG KLNT +  
Sbjct: 1   MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 60

Query: 89  TLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGFLRSDAFFRFIAY-SLIGGISTFS 147
            L       I +W+++      F F                   R IA   +  G +  +
Sbjct: 61  VLGMVSLNVIQIWSTLWVFMASFIF----------------CLIRVIAILGITFGATVSA 104

Query: 148 AMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLV 186
           A V D      LH        T +       L  LW L 
Sbjct: 105 AFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLF 143
>dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:1333114,
           evidence:ISS~phosphatidylinositol glycan, class
           Q~putative [Mus musculus]
          Length = 378

 Score = 38.0 bits (88), Expect = 0.19
 Identities = 10/50 (20%), Positives = 17/50 (34%), Gaps = 2/50 (4%)

Query: 13  TLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTG 60
             +  + L+R  +    +LLDVA  + L +    N       N       
Sbjct: 265 KAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVAD 314
CPU time:   103.60 user secs.	    2.38 sys. secs	  105.98 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.319    0.179    0.524 

Gapped
Lambda     K      H
   0.270   0.0628    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 208284706
Number of Sequences: 887402
Number of extensions: 11960944
Number of successful extensions: 50985
Number of sequences better than 10.0: 286
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 264
Number of HSP's that attempted gapping in prelim test: 50658
Number of HSP's gapped (non-prelim): 454
length of query: 287
length of database: 277,845,442
effective HSP length: 49
effective length of query: 238
effective length of database: 234,362,744
effective search space: 55778333072
effective search space used: 55778333072
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 73 (32.4 bits)