Sequences with E-value BETTER than threshold (0.002)
ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 355 6e-97
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 353 3e-96
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 351 6e-96
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 351 7e-96
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 351 9e-96
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 339 4e-92
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 338 6e-92
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 335 4e-91
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 318 7e-86
sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 286 4e-76
emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 277 2e-73
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 276 4e-73
emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 258 1e-67
ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 253 3e-66
ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 233 2e-60
emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 197 3e-49
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 130 3e-29
Sequences with E-value WORSE than threshold
dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:13331... 38 0.19
Alignments
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
Length = 287
Score = 355 bits (903), Expect = 6e-97
Identities = 287/287 (100%), Positives = 287/287 (100%)
Query: 1 MDKITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTG 60
MDKITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTG
Sbjct: 1 MDKITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTG 60
Query: 61 NVADNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFS 120
NVADNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFS
Sbjct: 61 NVADNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFS 120
Query: 121 AFIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLT 180
AFIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLT
Sbjct: 121 AFIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLT 180
Query: 181 VLWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVS 240
VLWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVS
Sbjct: 181 VLWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVS 240
Query: 241 ALQTVLYFFATWPFQLFALEKHLAEKYGKPADAQNEALAEKKTKSQN 287
ALQTVLYFFATWPFQLFALEKHLAEKYGKPADAQNEALAEKKTKSQN
Sbjct: 241 ALQTVLYFFATWPFQLFALEKHLAEKYGKPADAQNEALAEKKTKSQN 287
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
N-acetylglucosaminyl transferase component Gpi1 [Homo
sapiens]
gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
sapiens]
dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
Length = 581
Score = 353 bits (897), Expect = 3e-96
Identities = 84/279 (30%), Positives = 128/279 (45%), Gaps = 24/279 (8%)
Query: 12 RTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWT--PNWEWTVNEFWDQTGNVADNLNGT 69
R E L+R + +LLDVA + L + + +VA+ L
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323
Query: 70 ITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLR 127
+ WL PAGLK+N +++ L FF YHI+LW S + F + +H SA +
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACL---- 379
Query: 128 SDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVR 187
G++ +++ D + H CF Y +L L + L+ LW L R
Sbjct: 380 --------------GLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFR 425
Query: 188 GKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLY 247
GKKWN LR+R D+ D Q F+ T LF ILLF+LPT +Y++VF LRL V A+Q +++
Sbjct: 426 GKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIH 485
Query: 248 FFATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
L++L L Y A + L + ++
Sbjct: 486 LLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASR 524
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
sapiens]
gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
sapiens]
Length = 760
Score = 351 bits (894), Expect = 6e-96
Identities = 82/265 (30%), Positives = 122/265 (45%), Gaps = 24/265 (9%)
Query: 12 RTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWT--PNWEWTVNEFWDQTGNVADNLNGT 69
R E L+R + +LLDVA + L + + +VA+ L
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323
Query: 70 ITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLR 127
+ WL PAGLK+N +++ L FF YHI+LW S + F + +H SA +
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACL---- 379
Query: 128 SDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVR 187
G++ +++ D + H CF Y +L L + L+ LW L R
Sbjct: 380 --------------GLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFR 425
Query: 188 GKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLY 247
GKKWN LR+R D+ D Q F+ T LF ILLF+LPT +Y++VF LRL V A+Q +++
Sbjct: 426 GKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIH 485
Query: 248 FFATW--PFQLFALEKHLAEKYGKP 270
L++L L Y
Sbjct: 486 LLVDLINSLPLYSLGLRLCRPYRLA 510
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
component)) [Homo sapiens]
Length = 583
Score = 351 bits (894), Expect = 7e-96
Identities = 84/279 (30%), Positives = 127/279 (45%), Gaps = 24/279 (8%)
Query: 12 RTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWT--PNWEWTVNEFWDQTGNVADNLNGT 69
R E L+R + +LLDVA + L + + +VA+ L
Sbjct: 266 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 325
Query: 70 ITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLR 127
+ WL PAGLK+N +++ L FF YHI+LW S + F + +H SA +
Sbjct: 326 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACL---- 381
Query: 128 SDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVR 187
G++ +++ D + H CF Y +L L + L+ LW L R
Sbjct: 382 --------------GLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFR 427
Query: 188 GKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLY 247
GKKWN LR+R D+ D Q F+ T LF ILLF+LPT +Y++VF LRL V A+Q +++
Sbjct: 428 GKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIH 487
Query: 248 FFATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
L++L L Y A + L + +
Sbjct: 488 LLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEAGR 526
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
sapiens]
Length = 581
Score = 351 bits (893), Expect = 9e-96
Identities = 84/279 (30%), Positives = 127/279 (45%), Gaps = 24/279 (8%)
Query: 12 RTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWT--PNWEWTVNEFWDQTGNVADNLNGT 69
R E L+R + +LLDVA + L + + +VA+ L
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323
Query: 70 ITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLR 127
+ WL PAGLK+N +++ L FF YHI+LW S + F + +H SA +
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACL---- 379
Query: 128 SDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVR 187
G++ +++ D + H CF Y +L L + L+ LW L R
Sbjct: 380 --------------GLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFR 425
Query: 188 GKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLY 247
GKKWN LR+R D+ D Q F+ T LF ILLF+LPT +Y++VF LRL V A+Q +++
Sbjct: 426 GKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIH 485
Query: 248 FFATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
L++L L Y A + L + +
Sbjct: 486 LLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEAGR 524
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
(human) [Mus musculus]
Length = 581
Score = 339 bits (862), Expect = 4e-92
Identities = 83/278 (29%), Positives = 126/278 (44%), Gaps = 24/278 (8%)
Query: 13 TLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTGNVADNLNGTI 70
+ + L+R + +LLDVA + L + N N VA+ L +
Sbjct: 265 KAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLL 324
Query: 71 TWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLRS 128
WL PAGLK+N +++ L FF YHI+LW S + F + +H SA +
Sbjct: 325 QWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACL----- 379
Query: 129 DAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRG 188
G++ ++ D + H CF Y +L L Y L+ LW L RG
Sbjct: 380 -------------GLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRG 426
Query: 189 KKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYF 248
KKWN LR+R D+ D Q F+ T LF IL+F+LPT +Y++VF LRL V +Q +++
Sbjct: 427 KKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHL 486
Query: 249 FATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
L++L L Y A + L ++ +
Sbjct: 487 LVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGR 524
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
musculus]
Length = 581
Score = 338 bits (860), Expect = 6e-92
Identities = 83/278 (29%), Positives = 127/278 (44%), Gaps = 24/278 (8%)
Query: 13 TLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTGNVADNLNGTI 70
++ + L+R + +LLDVA + L + N N VA+ L +
Sbjct: 265 KAQSPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLL 324
Query: 71 TWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLRS 128
WL PAGLK+N +++ L FF YHI+LW S + F + +H SA +
Sbjct: 325 QWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACL----- 379
Query: 129 DAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRG 188
G++ ++ D + H CF Y +L L Y L+ LW L RG
Sbjct: 380 -------------GLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRG 426
Query: 189 KKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYF 248
KKWN LR+R D+ D Q F+ T LF IL+F+LPT +Y++VF LRL V +Q +++
Sbjct: 427 KKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHL 486
Query: 249 FATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
L++L L Y A + L ++ +
Sbjct: 487 LVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGR 524
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
glycosylphosphatidylinositol homolog (S. cerevisiae)
[Mus musculus]
dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
Length = 581
Score = 335 bits (853), Expect = 4e-91
Identities = 83/278 (29%), Positives = 125/278 (44%), Gaps = 24/278 (8%)
Query: 13 TLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTGNVADNLNGTI 70
+ + L+R + +LLDVA + L + N N VA+ L +
Sbjct: 265 KAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLL 324
Query: 71 TWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLRS 128
WL PAGLK+N +++ L FF YHI+LW S + F + +H SA +
Sbjct: 325 QWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACL----- 379
Query: 129 DAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRG 188
G++ ++ D + H CF Y +L L Y L+ LW L RG
Sbjct: 380 -------------GLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRG 426
Query: 189 KKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYF 248
KKWN LR+R D+ D Q F+ T LF IL+F+LPT +Y +VF LRL V +Q +++
Sbjct: 427 KKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHL 486
Query: 249 FATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
L++L L Y A + L ++ +
Sbjct: 487 LVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGR 524
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
Length = 339
Score = 318 bits (808), Expect = 7e-86
Identities = 83/278 (29%), Positives = 126/278 (44%), Gaps = 24/278 (8%)
Query: 13 TLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTGNVADNLNGTI 70
+ + L+R + +LLDVA + L + N N VA+ L +
Sbjct: 23 KAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLL 82
Query: 71 TWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLRS 128
WL PAGLK+N +++ L FF YHI+LW S + F + +H SA +
Sbjct: 83 QWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACL----- 137
Query: 129 DAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRG 188
G++ ++ D + H CF Y +L L Y L+ LW L RG
Sbjct: 138 -------------GLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRG 184
Query: 189 KKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYF 248
KKWN LR+R D+ D Q F+ T LF IL+F+LPT +Y++VF LRL V +Q +++
Sbjct: 185 KKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHL 244
Query: 249 FATW--PFQLFALEKHLAEKYGKPADAQNEALAEKKTK 284
L++L L Y A + L ++ +
Sbjct: 245 LVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGR 282
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
- fission yeast (Schizosaccharomyces pombe)
emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
protein gpi1. [Schizosaccharomyces pombe]
Length = 653
Score = 286 bits (725), Expect = 4e-76
Identities = 71/270 (26%), Positives = 117/270 (43%), Gaps = 25/270 (9%)
Query: 21 VRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVA-DNLNGTITWLRSNPAG 79
+R ++ L+ D+ F I + + N V + T A N+ + WL PAG
Sbjct: 313 IRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAG 372
Query: 80 LKLNTPVNETLAWFFTYHIYLWTSM-LFCFQKFAFNFHNDFSAFIGFLRSDAFFRFIAYS 138
LKLN + + + + I +W++ L C F + +A S
Sbjct: 373 LKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPF-----------------LVQVVAIS 415
Query: 139 LIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLRERK 198
GG S A++ DF + +H + +++L + L L RGKK N LR R
Sbjct: 416 GFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRI 475
Query: 199 DTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAV----SALQTVLYFFATWPF 254
D+ D Q L T LF +L+F LPTI+V++ F R++V + +T+L F +P
Sbjct: 476 DSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFP- 534
Query: 255 QLFALEKHLAEKYGKPADAQNEALAEKKTK 284
LF + + Y P+ E ++ + K
Sbjct: 535 -LFVTMLRIKDPYRIPSGLNFEIVSFEPLK 563
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
Length = 473
Score = 277 bits (702), Expect = 2e-73
Identities = 70/261 (26%), Positives = 108/261 (40%), Gaps = 20/261 (7%)
Query: 1 MDKITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTG 60
++ I + V+ R + + S +++D A I L + + +
Sbjct: 186 INSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFLIQISH 245
Query: 61 NVADNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFS 120
+ L + L +P GLKLN +N F YHI LW++ L
Sbjct: 246 IIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFL--------------- 290
Query: 121 AFIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLT 180
I + F A+ + G + A++ D I LH +CF Y L + LT
Sbjct: 291 DLIEPIVRQVFLAIGAFGCL-GFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLT 349
Query: 181 VLWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVS 240
VLW +VRG ++N LR R + RQ +LAT F +LF+ PT VY+VVF L+
Sbjct: 350 VLWQVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTC 409
Query: 241 ALQTVLYFFA----TWPFQLF 257
A L F +P ++F
Sbjct: 410 ATLATLECFRRKLLNFPIEMF 430
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
Length = 517
Score = 276 bits (700), Expect = 4e-73
Identities = 70/270 (25%), Positives = 117/270 (42%), Gaps = 25/270 (9%)
Query: 21 VRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVA-DNLNGTITWLRSNPAG 79
+R ++ L+ D+ F I + + N V + T A N+ + WL PAG
Sbjct: 177 IRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAG 236
Query: 80 LKLNTPVNETLAWFFTYHIYLWTSM-LFCFQKFAFNFHNDFSAFIGFLRSDAFFRFIAYS 138
LKLN + + + + I +W++ L C F + +A S
Sbjct: 237 LKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPF-----------------LVQVVAIS 279
Query: 139 LIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLRERK 198
GG S A++ DF + +H + ++++ + L L RGKK N LR R
Sbjct: 280 GFGGASLMIALISDFLSVMTIHIHLLYLASSRMYNWQLRVIYSLLQLFRGKKRNVLRNRI 339
Query: 199 DTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAV----SALQTVLYFFATWPF 254
D+ D Q L T LF +L+F LPTI+V++ F R++V + +T+L F +P
Sbjct: 340 DSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFP- 398
Query: 255 QLFALEKHLAEKYGKPADAQNEALAEKKTK 284
LF + + Y P+ E ++ + K
Sbjct: 399 -LFVTMLRIKDPYRIPSGLNFEIVSFEPLK 427
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
Length = 481
Score = 258 bits (653), Expect = 1e-67
Identities = 68/250 (27%), Positives = 105/250 (41%), Gaps = 16/250 (6%)
Query: 1 MDKITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTG 60
++ I + + + + S ++LD I L I + +
Sbjct: 193 INSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLATQPGDFLIQISH 252
Query: 61 NVADNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFS 120
V D L G + L +P GLKLN +N F YHI LW++ L
Sbjct: 253 YVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFL--------------- 297
Query: 121 AFIGFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLT 180
FI L F + G + A++ D + LH +CF Y L + L+
Sbjct: 298 DFIEPLVRQVFLAIGMIGCL-GFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLS 356
Query: 181 VLWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVS 240
VLW +VRG ++N L+ R ++ RQ +LAT F +LF+LPT VY++VF L+
Sbjct: 357 VLWQVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTF 416
Query: 241 ALQTVLYFFA 250
A +V +F
Sbjct: 417 ATLSVFHFVR 426
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
N-acetylglucoaminylphosphatidylinositol, the first
intermediate in synthesis of
glycosylphosphatidylinositol (GPI) anchors; Gpi1p
[Saccharomyces cerevisiae]
sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
cerevisiae)
emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
Length = 609
Score = 253 bits (642), Expect = 3e-66
Identities = 61/259 (23%), Positives = 105/259 (39%), Gaps = 21/259 (8%)
Query: 24 FSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQ-TGNVADNLNGTITWLRSNPAGLKL 82
++ L++ D++F + L I N ++ V+ + D+L L +NP G+KL
Sbjct: 279 YNTIWLIINDISFGLILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKL 338
Query: 83 NTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGFLRSDAFFRFIAYSL-IG 141
N + L+ F + I F++ I + Y + +
Sbjct: 339 NAELANFLSELFLWVIE-------------FSYTTFIKRLIDPKTLSSLLTLTIYMMFLV 385
Query: 142 GISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLRERKDTV 201
G S ++ DF I F ++KL + + L++L GKK N LR R D
Sbjct: 386 GFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHN 445
Query: 202 ILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSAL----QTVLYFFATWPFQLF 257
Q L T LF+IL+F+ PT+ +++ + LR+ + + V+ +P LF
Sbjct: 446 YFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFP--LF 503
Query: 258 ALEKHLAEKYGKPADAQNE 276
AL L + P E
Sbjct: 504 ALLLRLKDPKRLPGGISIE 522
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
Length = 680
Score = 233 bits (591), Expect = 2e-60
Identities = 59/274 (21%), Positives = 99/274 (35%), Gaps = 22/274 (8%)
Query: 7 NVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVADN- 65
++ ++ E L + S + +D+ + N E + +D +
Sbjct: 312 SISCVKHAEEAAL--QRHSTWSAMAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGI 369
Query: 66 LNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGF 125
L WL PAG KLNT + L I +W+++ F F
Sbjct: 370 LRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIF------------ 417
Query: 126 LRSDAFFRFIAY-SLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWS 184
R IA + G + +A V D LH T + L LW
Sbjct: 418 ----CLIRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWR 473
Query: 185 LVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQT 244
L RG+K NPLR+R D+ +Q + + LF LL +LPT V+++ F ++++
Sbjct: 474 LFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICM 533
Query: 245 VLYFFATW--PFQLFALEKHLAEKYGKPADAQNE 276
++ F + + L + P E
Sbjct: 534 LIEFAISVIHATPYAEVMIWLVRRKRFPCGVWFE 567
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
Length = 669
Score = 197 bits (497), Expect = 3e-49
Identities = 53/236 (22%), Positives = 88/236 (36%), Gaps = 20/236 (8%)
Query: 11 IRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVADNLNGTI 70
+L+ + L + F + +N+ N + + Q L +
Sbjct: 349 YYKYREILLIRIINLIIDIFLGFLIFLLLYFNVI--NLHYISEK--AQIFYGTSTLTSIL 404
Query: 71 TWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGFLRSDA 130
L NP G KLN + + W + +
Sbjct: 405 GTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTI----------------PVNNST 448
Query: 131 FFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKK 190
F+ Y+ + G S F + V D+ + H + K+C L + + L+ L GKK
Sbjct: 449 VLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKK 508
Query: 191 WNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVL 246
WN L+ R DT + L T LF IL+F+ PTIFV +VF + L ++ + +L
Sbjct: 509 WNILKLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLL 564
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana (fragment)
emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
Length = 143
Score = 130 bits (326), Expect = 3e-29
Identities = 33/159 (20%), Positives = 49/159 (30%), Gaps = 18/159 (11%)
Query: 30 LLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVADN-LNGTITWLRSNPAGLKLNTPVNE 88
+ +D+ + N E + +D + L WL PAG KLNT +
Sbjct: 1 MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 60
Query: 89 TLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGFLRSDAFFRFIAY-SLIGGISTFS 147
L I +W+++ F F R IA + G + +
Sbjct: 61 VLGMVSLNVIQIWSTLWVFMASFIF----------------CLIRVIAILGITFGATVSA 104
Query: 148 AMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLV 186
A V D LH T + L LW L
Sbjct: 105 AFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLF 143
>dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:1333114,
evidence:ISS~phosphatidylinositol glycan, class
Q~putative [Mus musculus]
Length = 378
Score = 38.0 bits (88), Expect = 0.19
Identities = 10/50 (20%), Positives = 17/50 (34%), Gaps = 2/50 (4%)
Query: 13 TLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTG 60
+ + L+R + +LLDVA + L + N N
Sbjct: 265 KAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVAD 314
CPU time: 103.60 user secs. 2.38 sys. secs 105.98 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.319 0.179 0.524
Gapped
Lambda K H
0.270 0.0628 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 208284706
Number of Sequences: 887402
Number of extensions: 11960944
Number of successful extensions: 50985
Number of sequences better than 10.0: 286
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 264
Number of HSP's that attempted gapping in prelim test: 50658
Number of HSP's gapped (non-prelim): 454
length of query: 287
length of database: 277,845,442
effective HSP length: 49
effective length of query: 238
effective length of database: 234,362,744
effective search space: 55778333072
effective search space used: 55778333072
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 73 (32.4 bits)