IMP GPI Biosynthesis Report IMP-Bioinformatics
GPI Biosynthesis Main Page  
GPI Site Motif   GPI Site Prediction   Home Page B.E.  

GPI Anchor Biosynthesis Report: O14357 (PIG-Q family, Schizosaccharomyces pombe)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (653 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 17 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 1002 0.0
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 755 0.0
ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 556 e-157
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 392 e-107
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 390 e-107
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 384 e-105
ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 383 e-105
emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 380 e-104
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 378 e-103
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 375 e-102
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 372 e-102
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 370 e-101
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 365 2e-99
ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 271 3e-71
emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 262 1e-68
emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 257 4e-67
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 167 7e-40
Alignments
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
           - fission yeast (Schizosaccharomyces pombe)
 emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
           protein gpi1. [Schizosaccharomyces pombe]
          Length = 653

 Score = 1002 bits (2563), Expect = 0.0
 Identities = 653/653 (100%), Positives = 653/653 (100%)

Query: 1   MSTTTMNIASAPIEVKRIYWPKSTISYTDSGYLVGWKYSTNDLIVVTTITSYQSFANFLV 60
           MSTTTMNIASAPIEVKRIYWPKSTISYTDSGYLVGWKYSTNDLIVVTTITSYQSFANFLV
Sbjct: 1   MSTTTMNIASAPIEVKRIYWPKSTISYTDSGYLVGWKYSTNDLIVVTTITSYQSFANFLV 60

Query: 61  DYCKNQDQSVDIKSLCILGTFNCSADILPGDKYEIDCWIKVQQSTELSHPRVFDSASTPL 120
           DYCKNQDQSVDIKSLCILGTFNCSADILPGDKYEIDCWIKVQQSTELSHPRVFDSASTPL
Sbjct: 61  DYCKNQDQSVDIKSLCILGTFNCSADILPGDKYEIDCWIKVQQSTELSHPRVFDSASTPL 120

Query: 121 KINLHIIYYHPPQPRKMQFLSLEPLSLLLLKDSFINKSNPEYESMQHQQILLKKLKLHFP 180
           KINLHIIYYHPPQPRKMQFLSLEPLSLLLLKDSFINKSNPEYESMQHQQILLKKLKLHFP
Sbjct: 121 KINLHIIYYHPPQPRKMQFLSLEPLSLLLLKDSFINKSNPEYESMQHQQILLKKLKLHFP 180

Query: 181 RRKENSWKRSLRSGLIELLNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFA 240
           RRKENSWKRSLRSGLIELLNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFA
Sbjct: 181 RRKENSWKRSLRSGLIELLNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFA 240

Query: 241 YFIILLRIINEVILLAINYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMKLWVFRK 300
           YFIILLRIINEVILLAINYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMKLWVFRK
Sbjct: 241 YFIILLRIINEVILLAINYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMKLWVFRK 300

Query: 301 SKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVR 360
           SKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVR
Sbjct: 301 SKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVR 360

Query: 361 SMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGA 420
           SMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGA
Sbjct: 361 SMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGA 420

Query: 421 SLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEY 480
           SLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEY
Sbjct: 421 SLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEY 480

Query: 481 DLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTML 540
           DLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTML
Sbjct: 481 DLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTML 540

Query: 541 RIKDPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYL 600
           RIKDPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYL
Sbjct: 541 RIKDPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYL 600

Query: 601 SKTTLISLLVGCPVPAIPAEQLYNIQYAMLPTKRISIRKLRDLLFHQKKFPYD 653
           SKTTLISLLVGCPVPAIPAEQLYNIQYAMLPTKRISIRKLRDLLFHQKKFPYD
Sbjct: 601 SKTTLISLLVGCPVPAIPAEQLYNIQYAMLPTKRISIRKLRDLLFHQKKFPYD 653
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
          Length = 517

 Score =  755 bits (1929), Expect = 0.0
 Identities = 516/517 (99%), Positives = 517/517 (99%)

Query: 137 MQFLSLEPLSLLLLKDSFINKSNPEYESMQHQQILLKKLKLHFPRRKENSWKRSLRSGLI 196
           MQFLSLEPLSLLLLKDSFINKSNPEYESMQHQQILLKKLKLHFPRRKENSWKRSLRSGLI
Sbjct: 1   MQFLSLEPLSLLLLKDSFINKSNPEYESMQHQQILLKKLKLHFPRRKENSWKRSLRSGLI 60

Query: 197 ELLNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFAYFIILLRIINEVILLA 256
           ELLNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFAYFIILLRIINEVILLA
Sbjct: 61  ELLNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFAYFIILLRIINEVILLA 120

Query: 257 INYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMKLWVFRKSKRVAIEDYKEYIRFY 316
           INYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMKLWVFRKSKRVAIEDYKEYIRFY
Sbjct: 121 INYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMKLWVFRKSKRVAIEDYKEYIRFY 180

Query: 317 NNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLN 376
           NNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLN
Sbjct: 181 NNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLN 240

Query: 377 NDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIALISDFLSVMTI 436
           NDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIALISDFLSVMTI
Sbjct: 241 NDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIALISDFLSVMTI 300

Query: 437 HIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLI 496
           HIHLLYLASSR+YNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLI
Sbjct: 301 HIHLLYLASSRMYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLI 360

Query: 497 FFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEI 556
           FFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEI
Sbjct: 361 FFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEI 420

Query: 557 VSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKTTLISLLVGCPVPA 616
           VSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKTTLISLLVGCPVPA
Sbjct: 421 VSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKTTLISLLVGCPVPA 480

Query: 617 IPAEQLYNIQYAMLPTKRISIRKLRDLLFHQKKFPYD 653
           IPAEQLYNIQYAMLPTKRISIRKLRDLLFHQKKFPYD
Sbjct: 481 IPAEQLYNIQYAMLPTKRISIRKLRDLLFHQKKFPYD 517
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi1p
           [Saccharomyces cerevisiae]
 sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
 gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
          Length = 609

 Score =  556 bits (1418), Expect = e-157
 Identities = 159/553 (28%), Positives = 285/553 (50%), Gaps = 67/553 (12%)

Query: 127 IYYHPPQPRKMQFLSLEPLSLLLLKDSFINKSNPEYESMQH----QQILLKKLKLHFPRR 182
           +++  P+ + MQF S +P+SL++          PE E   H    + +   KL+ H PR 
Sbjct: 77  VHFRIPKLKFMQFYSSDPISLII----------PEKEVGLHSSVGETLNYSKLEQH-PRY 125

Query: 183 KENSWKRSLRSGLIEL-------LNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFF 235
           K ++ K S    +I L       LN+ +     + E  N + + +  +    SS+  Y  
Sbjct: 126 KRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQE--NLRGTMLNSVAAWCSSTCIYKM 183

Query: 236 NSLFAYF-IILLRIINEVILLAINYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMK 294
            +   ++   ++  I  ++   +NY      + +++     +Q+DLR QQ C++PVQY +
Sbjct: 184 VAKIGFYLTFVICSIASLVSSLLNYSH----FQLVNYSAFVQQIDLRCQQICYFPVQYER 239

Query: 295 LWVFRKSKRVAIED-----------------------YKEYIRFYNNLWLVANDMIFGIT 331
           +     +K+  I++                       Y +YI  YN +WL+ ND+ FG+ 
Sbjct: 240 I-----NKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGLI 294

Query: 332 MSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVI 391
           + + ++EN   +V     +   +   +++++   L + P G+KLN ++  F+ +L +WVI
Sbjct: 295 LGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVI 354

Query: 392 DV-WSNFLLHCL---PWTPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSR 447
           +  ++ F+   +     +  L   + +    G S  ++L  DF ++++  I++ Y  SS+
Sbjct: 355 EFSYTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSK 414

Query: 448 LYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYA 507
           LY+ QL ++ SL  LF GKKRNVLRNRID   + LDQLLLGT+LF +L+F  PT+  FY 
Sbjct: 415 LYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYM 474

Query: 508 AFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEIVSFEPLKQDGF 567
           ++ + R+  +T     E ++A +NHFPLF  +LR+KDP R+P G++ E+ +    K    
Sbjct: 475 SYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISIELKTTVSNKH--- 531

Query: 568 ATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKTTLISLLVGCPVPAIPAEQLYNIQY 627
            TL L  N  P+   SMF  Y  L  ++ ++Y S  T+  ++ G  +  +   +LY + Y
Sbjct: 532 TTLELQNN--PIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESI-MVNRNKLYYVLY 588

Query: 628 AMLPTKRISIRKL 640
           + LP+K +S++ L
Sbjct: 589 SSLPSKPLSVKDL 601
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
           sapiens]
          Length = 581

 Score =  392 bits (997), Expect = e-107
 Identities = 88/299 (29%), Positives = 163/299 (54%), Gaps = 15/299 (5%)

Query: 295 LWVFRKSKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENL---HLVVKLIENIT 351
           ++  RK+     E+  + +R  N +  V  D+  G+ + S++       HL   L+    
Sbjct: 260 IFSTRKA-----ENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVP--V 312

Query: 352 FEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQV 411
            ++  + ++ ++ WL+  PAGLK+N  + + + +  ++ I +W +++    P+   ++  
Sbjct: 313 ADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWH 372

Query: 412 VAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVL 471
           V +S   G ++ ++L+SD ++++T HI+  Y+  +RLY  ++  + SL +LFRGKK NVL
Sbjct: 373 VGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVL 432

Query: 472 RNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLN 531
           R R+DS  YDLDQL +GT+LFT+L+F LPT  ++Y  F L R+ V+    +   ++  +N
Sbjct: 433 RQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLIN 492

Query: 532 HFPLFVTMLRIKDPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRK 590
             PL+   LR+  PYR+ +G+ F +     L+ +    L L     P+    +   YR 
Sbjct: 493 SLPLYSLGLRLCRPYRLAAGVKFRV-----LRHEAGRPLRLLMQINPLPYSRVVHTYRL 546
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
           N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
          Length = 581

 Score =  390 bits (992), Expect = e-107
 Identities = 88/299 (29%), Positives = 163/299 (54%), Gaps = 15/299 (5%)

Query: 295 LWVFRKSKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENL---HLVVKLIENIT 351
           ++  RK+     E+  + +R  N +  V  D+  G+ + S++       HL   L+    
Sbjct: 260 IFSTRKA-----ENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVP--V 312

Query: 352 FEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQV 411
            ++  + ++ ++ WL+  PAGLK+N  + + + +  ++ I +W +++    P+   ++  
Sbjct: 313 ADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWH 372

Query: 412 VAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVL 471
           V +S   G ++ ++L+SD ++++T HI+  Y+  +RLY  ++  + SL +LFRGKK NVL
Sbjct: 373 VGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVL 432

Query: 472 RNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLN 531
           R R+DS  YDLDQL +GT+LFT+L+F LPT  ++Y  F L R+ V+    +   ++  +N
Sbjct: 433 RQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLIN 492

Query: 532 HFPLFVTMLRIKDPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRK 590
             PL+   LR+  PYR+ +G+ F +     L+ +    L L     P+    +   YR 
Sbjct: 493 SLPLYSLGLRLCRPYRLAAGVKFRV-----LRHEASRPLRLLMQINPLPYSRVVHTYRL 546
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
           component)) [Homo sapiens]
          Length = 583

 Score =  384 bits (978), Expect = e-105
 Identities = 91/349 (26%), Positives = 178/349 (50%), Gaps = 16/349 (4%)

Query: 246 LRIINEVILLAINYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMK-LWVFRKSKRV 304
           + ++   +L  ++              +S     L   +     ++++  ++  RK+   
Sbjct: 212 ICLLLASLLSLVSAVSACRRRVFKLWPLSFLGSKLSTCEQLRHRLEHLTLIFSTRKA--- 268

Query: 305 AIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENL---HLVVKLIENITFEYAIKNVRS 361
             E+  + +R  N +  V  D+  G+ + S++       HL   L+     ++  + ++ 
Sbjct: 269 --ENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVP--VADHVAEELQH 324

Query: 362 MVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGAS 421
           ++ WL+  PAGLK+N  + + + +  ++ I +W +++    P+   ++  V +S   G +
Sbjct: 325 LLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLT 384

Query: 422 LMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYD 481
           + ++L+SD ++++T HI+  Y+  +RLY  ++  + SL +LFRGKK NVLR R+DS  YD
Sbjct: 385 VALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYD 444

Query: 482 LDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLR 541
           LDQL +GT+LFT+L+F LPT  ++Y  F L R+ V+    +   ++  +N  PL+   LR
Sbjct: 445 LDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLR 504

Query: 542 IKDPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRK 590
           +  PYR+ +G+ F +     L+ +    L L     P+    +   YR 
Sbjct: 505 LCRPYRLAAGVKFRV-----LRHEAGRPLRLLMQINPLPYSRVVHTYRL 548
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
 pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
 emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
          Length = 680

 Score =  383 bits (974), Expect = e-105
 Identities = 95/367 (25%), Positives = 181/367 (48%), Gaps = 29/367 (7%)

Query: 196 IELLNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFAYFIILLRIINEVILL 255
           +E  +Q+F +  L        +S  +RL   +  S    + SL  +F +L         L
Sbjct: 225 LETSSQNFSISYLI-------SSLTWRLLATILGSLSSLYYSLAQFFYLLSS------FL 271

Query: 256 AINYRPIPLSYNMMDIFVSARQVDLRLQQACFWP-----VQYMKLWVFRKSKRVAIEDYK 310
             ++  I     + + +++ R   +R  Q  +WP     +  M +   + ++  A++ + 
Sbjct: 272 IFSWVHIASRRVLKNTWINFR---IRSCQILYWPIFLEEIDMMSISCVKHAEEAALQRHS 328

Query: 311 EYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTP 370
            +         +A D++ G  +   +L N   V   + +   E+    +RS  +WL+  P
Sbjct: 329 TWSA-------MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVP 381

Query: 371 AGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFG-GASLMIALISD 429
           AG KLN ++   +  +S+ VI +WS   +    +   L++V+AI G   GA++  A + D
Sbjct: 382 AGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVID 441

Query: 430 FLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGT 489
            ++  T+HI  L+ A + +Y+ Q++ + +L +LFRG+K N LR R+DSY Y + Q ++G+
Sbjct: 442 VITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTVKQHVVGS 501

Query: 490 ILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIP 549
           +LFT L+  LPT  VFY  F +T  ++ +   + E  ++ ++  P    M+ +    R P
Sbjct: 502 LLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWLVRRKRFP 561

Query: 550 SGLNFEI 556
            G+ FE+
Sbjct: 562 CGVWFEM 568
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
          Length = 669

 Score =  380 bits (967), Expect = e-104
 Identities = 98/417 (23%), Positives = 181/417 (42%), Gaps = 38/417 (9%)

Query: 179 FPRRKENSWKRSLRSGLIEL------LNQSFEVRMLTHENNNK----------KNSYVFR 222
           + + KEN  K + +   I        L +SF +  + H  N K          KN Y   
Sbjct: 197 YNKDKENKLKLNDKDEYISFNFIEDKLTESFHMNQIIHLINKKCVFTKCLENYKNRYFVL 256

Query: 223 LFDRVSS--------STFYFFNSLFAYFIILLRIINEVILLAINYRPIPLSYNMMDIFVS 274
             + +          S F +  S+  +F  ++ +IN  +   I       +  +   ++ 
Sbjct: 257 KKEEILKKKKKQKKMSIFSYIVSIILFFTYIISLINSCLYYII------CTPKLFSEYIF 310

Query: 275 ARQVDLRLQQACF----WPVQYMKLW-VFRKSKRVAIEDYKEYIRFYNNLWLVANDMIFG 329
           +++ D  LQ + +    +P ++  ++  F K+K+   E YK        +  +  D+  G
Sbjct: 311 SKKCDGYLQNSAYPKFIFPSEWHNIFRSFMKNKQNPSEYYKYREILLIRIINLIIDIFLG 370

Query: 330 ITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVW 389
             +   +  N+  +  + E     Y    + S++  L+  P G KLNN+   FI  + V 
Sbjct: 371 FLIFLLLYFNVINLHYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNFTSFIGSILVS 430

Query: 390 VIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRLY 449
           ++D W  F          ++  V  +   G S  ++ + D+L  +T H+ ++YL   ++ 
Sbjct: 431 ILDKWDLFTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKIC 490

Query: 450 NWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAF 509
               + +YSL  LF GKK N+L+ R+D+  Y  +++LLGTILFT+LIF  PTI+V    F
Sbjct: 491 TLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLVF 550

Query: 510 ALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNF---EIVSFEPLK 563
            L  + +   + +   M   + + P ++  ++      I  G  F   E+   E LK
Sbjct: 551 GLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGFKFTKYEVGEHELLK 607
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
 gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
           (human) [Mus musculus]
          Length = 581

 Score =  378 bits (963), Expect = e-103
 Identities = 105/418 (25%), Positives = 199/418 (47%), Gaps = 39/418 (9%)

Query: 204 EVRMLTHENNNKKNSYVFRLFDRVSSS-TFYFFNSLFAYFIILLRIINEVILLAINYRPI 262
            VR+   ++   + S +  L  R S        + L          +         ++  
Sbjct: 185 PVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRL---------WKLW 235

Query: 263 PLSYNMMDIFVSARQVDLRLQQACFWPVQYMKLWVFRKSKRVAIEDYKEYIRFYNNLWLV 322
           PLS+ +     +  Q+  RL+   F       ++   K+     ++  + +R  N L  V
Sbjct: 236 PLSF-IRSKLSTCEQLHHRLKHLSF-------IFSTEKA-----QNPMQLMRKANMLVSV 282

Query: 323 ANDMIFGITMSSFILENLHL------VVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLN 376
             D+  G+ + S++  N  +      +V + + +  E     ++ ++ WL+  PAGLK+N
Sbjct: 283 LLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEE-----LQHLLQWLMGAPAGLKMN 337

Query: 377 NDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIALISDFLSVMTI 436
             + + + +  ++ I +W +++    P+   ++  V +S   G ++ +++ SD ++++T 
Sbjct: 338 RALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTF 397

Query: 437 HIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLI 496
           HI+  Y+  +RLY  ++  + SL +LFRGKK NVLR R+DS  YDLDQL +GT+LFT+L+
Sbjct: 398 HIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILV 457

Query: 497 FFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEI 556
           F LPT  ++Y  F L R+ V+T   +   ++  +N  PL+   LR+  PYR+ +G+ F +
Sbjct: 458 FLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRV 517

Query: 557 VSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKTTLISLLVGCPV 614
                L+++    L L     P+S   +   YR         +   T    L+ G  +
Sbjct: 518 -----LEKEAGRPLRLLMQINPLSYNHVMHIYRLPRCGCHPKHSWGTLCRKLVFGELI 570
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
           glycosylphosphatidylinositol homolog (S. cerevisiae)
           [Mus musculus]
 dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
          Length = 581

 Score =  375 bits (954), Expect = e-102
 Identities = 104/418 (24%), Positives = 198/418 (46%), Gaps = 39/418 (9%)

Query: 204 EVRMLTHENNNKKNSYVFRLFDRVSSS-TFYFFNSLFAYFIILLRIINEVILLAINYRPI 262
            VR+   ++   + S +  L  R S        + L          +         ++  
Sbjct: 185 PVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRL---------WKLW 235

Query: 263 PLSYNMMDIFVSARQVDLRLQQACFWPVQYMKLWVFRKSKRVAIEDYKEYIRFYNNLWLV 322
           PLS+ +     +  Q+  RL+   F       ++   K+     ++  + +R  N L  V
Sbjct: 236 PLSF-IRSKLSTCEQLHHRLKHLSF-------IFSTEKA-----QNPMQLMRKANMLVSV 282

Query: 323 ANDMIFGITMSSFILENLHL------VVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLN 376
             D+  G+ + S++  N  +      +V + + +  E     ++ ++ WL+  PAGLK+N
Sbjct: 283 LLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEE-----LQHLLQWLMGAPAGLKMN 337

Query: 377 NDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIALISDFLSVMTI 436
             + + + +  ++ I +W +++    P+   ++  V +S   G ++ +++ SD ++++T 
Sbjct: 338 RALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTF 397

Query: 437 HIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLI 496
           HI+  Y+  +RLY  ++  + SL +LFRGKK NVLR R+DS  YDLDQL +GT+LFT+L+
Sbjct: 398 HIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILV 457

Query: 497 FFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEI 556
           F LPT  ++   F L R+ V+T   +   ++  +N  PL+   LR+  PYR+ +G+ F +
Sbjct: 458 FLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRV 517

Query: 557 VSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKTTLISLLVGCPV 614
                L+++    L L     P+S   +   YR         +   T    L+ G  +
Sbjct: 518 -----LEKEAGRPLRLLMQINPLSYNHVMHIYRLPRCGCHPKHSWGTLCRKLVFGELI 570
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
          Length = 339

 Score =  372 bits (947), Expect = e-102
 Identities = 90/314 (28%), Positives = 167/314 (52%), Gaps = 16/314 (5%)

Query: 307 EDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHL------VVKLIENITFEYAIKNVR 360
           ++  + +R  N L  V  D+  G+ + S++  N  +      +V + + +  E     ++
Sbjct: 25  QNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEE-----LQ 79

Query: 361 SMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGA 420
            ++ WL+  PAGLK+N  + + + +  ++ I +W +++    P+   ++  V +S   G 
Sbjct: 80  HLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGL 139

Query: 421 SLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEY 480
           ++ +++ SD ++++T HI+  Y+  +RLY  ++  + SL +LFRGKK NVLR R+DS  Y
Sbjct: 140 TVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSY 199

Query: 481 DLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTML 540
           DLDQL +GT+LFT+L+F LPT  ++Y  F L R+ V+T   +   ++  +N  PL+   L
Sbjct: 200 DLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGL 259

Query: 541 RIKDPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYL 600
           R+  PYR+ +G+ F +     L+++    L L     P+S   +   YR         + 
Sbjct: 260 RLCRPYRLAAGVKFRV-----LEKEAGRPLRLLMQINPLSYNHVMHIYRLPRCGCHPKHS 314

Query: 601 SKTTLISLLVGCPV 614
             T    L+ G  +
Sbjct: 315 WGTLCRKLVFGELI 328
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
           musculus]
          Length = 581

 Score =  370 bits (942), Expect = e-101
 Identities = 99/394 (25%), Positives = 187/394 (47%), Gaps = 39/394 (9%)

Query: 204 EVRMLTHENNNKKNSYVFRLFDRVSSS-TFYFFNSLFAYFIILLRIINEVILLAINYRPI 262
            VR+   ++   + S +  L  R S        + L          + ++  LA     +
Sbjct: 185 PVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLGPLAFIRSKL 244

Query: 263 PLSYNMMDIFVSARQVDLRLQQACFWPVQYMKLWVFRKSKRVAIEDYKEYIRFYNNLWLV 322
                +            RL+   F       ++   K+     +   + +R  N L  V
Sbjct: 245 STCEQLQH----------RLKHLSF-------IFSTEKA-----QSPMQLMRKANMLVSV 282

Query: 323 ANDMIFGITMSSFILENLHL------VVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLN 376
             D+  G+ + S++  N  +      +V + + +  E     ++ ++ WL+  PAGLK+N
Sbjct: 283 LLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEE-----LQHLLQWLMGAPAGLKMN 337

Query: 377 NDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIALISDFLSVMTI 436
             + + + +  ++ I +W +++    P+   ++  V +S   G ++ +++ SD ++++T 
Sbjct: 338 RALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTF 397

Query: 437 HIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLI 496
           HI+  Y+  +RLY  ++  + SL +LFRGKK NVLR R+DS  YDLDQL +GT+LFT+L+
Sbjct: 398 HIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILV 457

Query: 497 FFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEI 556
           F LPT  ++Y  F L R+ V+T   +   ++  +N  PL+   LR+  PYR+ +G+ F +
Sbjct: 458 FLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRV 517

Query: 557 VSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRK 590
                L+++    L L     P+    +   YR 
Sbjct: 518 -----LEKEAGRPLRLLMQINPLPYSHVVHTYRL 546
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
 gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 760

 Score =  365 bits (928), Expect = 2e-99
 Identities = 80/258 (31%), Positives = 148/258 (57%), Gaps = 10/258 (3%)

Query: 295 LWVFRKSKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENL---HLVVKLIENIT 351
           ++  RK+     E+  + +R  N +  V  D+  G+ + S++       HL   L+    
Sbjct: 260 IFSTRKA-----ENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVP--V 312

Query: 352 FEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQV 411
            ++  + ++ ++ WL+  PAGLK+N  + + + +  ++ I +W +++    P+   ++  
Sbjct: 313 ADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWH 372

Query: 412 VAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVL 471
           V +S   G ++ ++L+SD ++++T HI+  Y+  +RLY  ++  + SL +LFRGKK NVL
Sbjct: 373 VGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVL 432

Query: 472 RNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLN 531
           R R+DS  YDLDQL +GT+LFT+L+F LPT  ++Y  F L R+ V+    +   ++  +N
Sbjct: 433 RQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLIN 492

Query: 532 HFPLFVTMLRIKDPYRIP 549
             PL+   LR+  PYR+ 
Sbjct: 493 SLPLYSLGLRLCRPYRLA 510
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
 pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
 emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
          Length = 287

 Score =  271 bits (686), Expect = 3e-71
 Identities = 69/258 (26%), Positives = 113/258 (43%), Gaps = 25/258 (9%)

Query: 312 YIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPA 371
            +R ++   L+  D+ F I + +    N    V    + T   A  N+   + WL   PA
Sbjct: 20  LVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVA-DNLNGTITWLRSNPA 78

Query: 372 GLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPF-----------------LVQVVAI 414
           GLKLN  + + +     + I +W++ +L C     F                   + +A 
Sbjct: 79  GLKLNTPVNETLAWFFTYHIYLWTS-MLFCFQKFAFNFHNDFSAFIGFLRSDAFFRFIAY 137

Query: 415 SGFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNR 474
           S  GG S   A++ DF  +  +H +     +++L       +  L  L RGKK N LR R
Sbjct: 138 SLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLRER 197

Query: 475 IDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFP 534
            D+   D  Q  L T LF +L+F LPTI+V++  F   R++V    +  +T+L F   +P
Sbjct: 198 KDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAV----SALQTVLYFFATWP 253

Query: 535 --LFVTMLRIKDPYRIPS 550
             LF     + + Y  P+
Sbjct: 254 FQLFALEKHLAEKYGKPA 271
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
          Length = 481

 Score =  262 bits (665), Expect = 1e-68
 Identities = 63/233 (27%), Positives = 111/233 (47%), Gaps = 5/233 (2%)

Query: 312 YIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPA 371
             R  N +      +I  + + S   +    ++++       Y I  +  ++  L  +P 
Sbjct: 216 RSRPANIVLDRILGIILMLILFSLATQPGDFLIQISH-----YVIDELYGLLKVLEGSPI 270

Query: 372 GLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIALISDFL 431
           GLKLN  +  F +    + I++WS FL    P    +   + + G  G +  IAL+ D +
Sbjct: 271 GLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTFQIALLVDLI 330

Query: 432 SVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTIL 491
           SV+ +H H  Y+ +  LYN + R +  L Q+ RG + N+L+ R +S+ Y   QL L TI 
Sbjct: 331 SVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMNRQLYLATIF 390

Query: 492 FTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKD 544
           F+ ++F LPT  V+Y  FA  +      L++   +   L + P+ V + R+  
Sbjct: 391 FSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRLLR 443
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
          Length = 473

 Score =  257 bits (651), Expect = 4e-67
 Identities = 69/238 (28%), Positives = 113/238 (46%), Gaps = 4/238 (1%)

Query: 309 YKEYIRFY---NNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIW 365
           YKE+   Y   + L  +  D   GI +   +   L               I  + S++  
Sbjct: 199 YKEWQSIYTKGSRLSNIMIDRALGIILMLVL-FTLVSHPGDFLIQISHIIIHQLYSLLKV 257

Query: 366 LVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIA 425
           L  +P GLKLN  +  F +    + I++WS FL    P    +   +   G  G +  IA
Sbjct: 258 LEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIA 317

Query: 426 LISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQL 485
           L++D +S++ +H H  Y+ +  L N +++ +  L Q+ RG + N+LRNRI+++ Y   QL
Sbjct: 318 LLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQL 377

Query: 486 LLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIK 543
            L TI F+ ++F  PT  V+Y  FA  +      LA  E     L +FP+ + +  IK
Sbjct: 378 YLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIK 435
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana  (fragment)
 emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
          Length = 143

 Score =  167 bits (419), Expect = 7e-40
 Identities = 41/143 (28%), Positives = 78/143 (53%), Gaps = 1/143 (0%)

Query: 322 VANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICK 381
           +A D++ G  +   +L N   V   + +   E+    +RS  +WL+  PAG KLN ++  
Sbjct: 1   MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 60

Query: 382 FIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFG-GASLMIALISDFLSVMTIHIHL 440
            +  +S+ VI +WS   +    +   L++V+AI G   GA++  A + D ++  T+HI  
Sbjct: 61  VLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMA 120

Query: 441 LYLASSRLYNWQLRVIYSLLQLF 463
           L+ A + +Y+ Q++ + +L +LF
Sbjct: 121 LHWAITLVYSHQIQALAALWRLF 143
CPU time:   169.45 user secs.	    2.66 sys. secs	  172.11 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.317    0.163    0.475 

Gapped
Lambda     K      H
   0.270   0.0571    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 404420202
Number of Sequences: 887402
Number of extensions: 19851371
Number of successful extensions: 85573
Number of sequences better than 10.0: 238
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 219
Number of HSP's that attempted gapping in prelim test: 85173
Number of HSP's gapped (non-prelim): 510
length of query: 653
length of database: 277,845,442
effective HSP length: 55
effective length of query: 598
effective length of database: 229,038,332
effective search space: 136964922536
effective search space used: 136964922536
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 76 (33.7 bits)