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GPI Anchor Biosynthesis Report: NP_191276.1 (PIG-Q family, Arabidopsis thaliana)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (680 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 21 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 1058 0.0
sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 446 e-124
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 438 e-121
emb|CAB68123.2| (AL137080) hypothetical protein [Arabidopsis tha... 429 e-118
pir||T45795 hypothetical protein F28O9.20 - Arabidopsis thaliana 416 e-115
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 396 e-109
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 387 e-106
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 387 e-106
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 386 e-106
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 360 4e-98
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 357 4e-97
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 356 6e-97
ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 348 2e-94
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 347 5e-94
emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 279 1e-73
emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 277 5e-73
emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 269 9e-71
ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 214 4e-54
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 169 1e-40
Sequences with E-value WORSE than threshold

gb|AAH10094.1|AAH10094 (BC010094) Similar to phosphatidylinosito... 44 0.010
dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:13331... 35 2.7
Alignments
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
 pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
 emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
          Length = 680

 Score = 1058 bits (2707), Expect = 0.0
 Identities = 680/680 (100%), Positives = 680/680 (100%)

Query: 1   MKRKCRIWLPKQLASTDDLSHSLLFGWFVCHSSSCLDVVVSFISDESSLSNAGSKLQDVL 60
           MKRKCRIWLPKQLASTDDLSHSLLFGWFVCHSSSCLDVVVSFISDESSLSNAGSKLQDVL
Sbjct: 1   MKRKCRIWLPKQLASTDDLSHSLLFGWFVCHSSSCLDVVVSFISDESSLSNAGSKLQDVL 60

Query: 61  RETNEKMPSTLRDKAAFTLLGRYDISRNANGNVSKIIRDKDMSRKAGAYVRPCKYSGLSC 120
           RETNEKMPSTLRDKAAFTLLGRYDISRNANGNVSKIIRDKDMSRKAGAYVRPCKYSGLSC
Sbjct: 61  RETNEKMPSTLRDKAAFTLLGRYDISRNANGNVSKIIRDKDMSRKAGAYVRPCKYSGLSC 120

Query: 121 GCHKSIELWNSIQVLDCIIYTGMGILYLNAMSTYVIVYDTPVFGSHHFSLSFSNSSPQTK 180
           GCHKSIELWNSIQVLDCIIYTGMGILYLNAMSTYVIVYDTPVFGSHHFSLSFSNSSPQTK
Sbjct: 121 GCHKSIELWNSIQVLDCIIYTGMGILYLNAMSTYVIVYDTPVFGSHHFSLSFSNSSPQTK 180

Query: 181 APLKKPKWVDDLHNRKPLNEMETVILSLNCAAAAKIAYKKISTQLETSSQNFSISYLISS 240
           APLKKPKWVDDLHNRKPLNEMETVILSLNCAAAAKIAYKKISTQLETSSQNFSISYLISS
Sbjct: 181 APLKKPKWVDDLHNRKPLNEMETVILSLNCAAAAKIAYKKISTQLETSSQNFSISYLISS 240

Query: 241 LTWRLLATILGSLSSLYYSLAQFFYLLSSFLIFSWVHIASRRVLKNTWINFRIRSCQILY 300
           LTWRLLATILGSLSSLYYSLAQFFYLLSSFLIFSWVHIASRRVLKNTWINFRIRSCQILY
Sbjct: 241 LTWRLLATILGSLSSLYYSLAQFFYLLSSFLIFSWVHIASRRVLKNTWINFRIRSCQILY 300

Query: 301 WPIFLEEIDMMSISCVKHAEEAALQRHSTWSAMAVDLVLGNLIGLGLLFNTESVCSFVFD 360
           WPIFLEEIDMMSISCVKHAEEAALQRHSTWSAMAVDLVLGNLIGLGLLFNTESVCSFVFD
Sbjct: 301 WPIFLEEIDMMSISCVKHAEEAALQRHSTWSAMAVDLVLGNLIGLGLLFNTESVCSFVFD 360

Query: 361 FAKEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLI 420
           FAKEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLI
Sbjct: 361 FAKEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLI 420

Query: 421 RVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKL 480
           RVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKL
Sbjct: 421 RVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKL 480

Query: 481 NPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAIS 540
           NPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAIS
Sbjct: 481 NPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAIS 540

Query: 541 VIHATPYAEVMIWLVRRKRFPCGVWFEMEHCGEHILKSNDAFEDSKSLLEEHGTPEKNSL 600
           VIHATPYAEVMIWLVRRKRFPCGVWFEMEHCGEHILKSNDAFEDSKSLLEEHGTPEKNSL
Sbjct: 541 VIHATPYAEVMIWLVRRKRFPCGVWFEMEHCGEHILKSNDAFEDSKSLLEEHGTPEKNSL 600

Query: 601 MVSNLRSNFLTLGQILLPHYKTIFSGISASSLTTSARGVLSGKRMPSKLGLDLPPPRPWL 660
           MVSNLRSNFLTLGQILLPHYKTIFSGISASSLTTSARGVLSGKRMPSKLGLDLPPPRPWL
Sbjct: 601 MVSNLRSNFLTLGQILLPHYKTIFSGISASSLTTSARGVLSGKRMPSKLGLDLPPPRPWL 660

Query: 661 HMPLRQYWMLCHNSISSCGK 680
           HMPLRQYWMLCHNSISSCGK
Sbjct: 661 HMPLRQYWMLCHNSISSCGK 680
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
           - fission yeast (Schizosaccharomyces pombe)
 emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
           protein gpi1. [Schizosaccharomyces pombe]
          Length = 653

 Score =  446 bits (1135), Expect = e-124
 Identities = 108/457 (23%), Positives = 206/457 (44%), Gaps = 31/457 (6%)

Query: 222 STQLETSSQNFSISYLI-------SSLTWRLLATILGSLSSLYYSLAQFFYLLSS----- 269
           S  +E  +Q+F +  L        +S  +RL   +  S    + SL  +F +L       
Sbjct: 193 SGLIELLNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFAYFIILLRIINEV 252

Query: 270 -FLIFSWVHIASRRVLKNTWINFR---IRSCQILYWPIFLEEIDMMSISCVKHAEEAALQ 325
             L  ++  I     + + +++ R   +R  Q  +WP     +  M +   + ++  A++
Sbjct: 253 ILLAINYRPIPLSYNMMDIFVSARQVDLRLQQACFWP-----VQYMKLWVFRKSKRVAIE 307

Query: 326 RHSTWSA-------MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLM 378
            +  +         +A D++ G  +   +L N   V   + +   E+    +RS  +WL+
Sbjct: 308 DYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLV 367

Query: 379 GVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAF 438
             PAG KLN ++   +  +S+ VI +WS   +    +   L++V+AI G   GA++  A 
Sbjct: 368 DTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGF-GGASLMIAL 426

Query: 439 VIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTVKQHV 498
           + D ++  T+HI  L+ A + +Y+ Q++ + +L +LFRG+K N LR R+DSY Y + Q +
Sbjct: 427 ISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLL 486

Query: 499 VGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWLVRRK 558
           +G++LFT L+  LPT  VFY  F +T  ++ +   + E  ++ ++  P    M+ +    
Sbjct: 487 LGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPY 546

Query: 559 RFPCGVWFEMEHCGEHILKSNDAFEDSKS--LLEEHGTPEKNSLMVSNLRSNFLTLGQIL 616
           R P G+ FE+                + +   +      E    +   L S++L+   ++
Sbjct: 547 RIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKTTLI 606

Query: 617 LPHYKTIFSGISASSLTTSARGVLSGKRMPSKLGLDL 653
                     I A  L      +L  KR+  +   DL
Sbjct: 607 SLLVGCPVPAIPAEQLYNIQYAMLPTKRISIRKLRDL 643
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
          Length = 517

 Score =  438 bits (1115), Expect = e-121
 Identities = 108/457 (23%), Positives = 206/457 (44%), Gaps = 31/457 (6%)

Query: 222 STQLETSSQNFSISYLI-------SSLTWRLLATILGSLSSLYYSLAQFFYLLSS----- 269
           S  +E  +Q+F +  L        +S  +RL   +  S    + SL  +F +L       
Sbjct: 57  SGLIELLNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFAYFIILLRIINEV 116

Query: 270 -FLIFSWVHIASRRVLKNTWINFR---IRSCQILYWPIFLEEIDMMSISCVKHAEEAALQ 325
             L  ++  I     + + +++ R   +R  Q  +WP     +  M +   + ++  A++
Sbjct: 117 ILLAINYRPIPLSYNMMDIFVSARQVDLRLQQACFWP-----VQYMKLWVFRKSKRVAIE 171

Query: 326 RHSTWSA-------MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLM 378
            +  +         +A D++ G  +   +L N   V   + +   E+    +RS  +WL+
Sbjct: 172 DYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLV 231

Query: 379 GVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAF 438
             PAG KLN ++   +  +S+ VI +WS   +    +   L++V+AI G   GA++  A 
Sbjct: 232 DTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGF-GGASLMIAL 290

Query: 439 VIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTVKQHV 498
           + D ++  T+HI  L+ A + +Y+ Q++ + +L +LFRG+K N LR R+DSY Y + Q +
Sbjct: 291 ISDFLSVMTIHIHLLYLASSRMYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLL 350

Query: 499 VGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWLVRRK 558
           +G++LFT L+  LPT  VFY  F +T  ++ +   + E  ++ ++  P    M+ +    
Sbjct: 351 LGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPY 410

Query: 559 RFPCGVWFEMEHCGEHILKSNDAFEDSKS--LLEEHGTPEKNSLMVSNLRSNFLTLGQIL 616
           R P G+ FE+                + +   +      E    +   L S++L+   ++
Sbjct: 411 RIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKTTLI 470

Query: 617 LPHYKTIFSGISASSLTTSARGVLSGKRMPSKLGLDL 653
                     I A  L      +L  KR+  +   DL
Sbjct: 471 SLLVGCPVPAIPAEQLYNIQYAMLPTKRISIRKLRDL 507
>emb|CAB68123.2| (AL137080) hypothetical protein [Arabidopsis thaliana]
          Length = 261

 Score =  429 bits (1092), Expect = e-118
 Identities = 212/264 (80%), Positives = 218/264 (82%), Gaps = 30/264 (11%)

Query: 1   MKRKCRIWLPKQLASTDDLSHSLLFGWFVCHSSSCLDVVVSFISDESSLSNAGSKLQDVL 60
           MKRKCRIWLPKQLASTDDLSHSLLFGWFVCHSSSCLDVVVSFISDESSLSNAGSKLQDVL
Sbjct: 1   MKRKCRIWLPKQLASTDDLSHSLLFGWFVCHSSSCLDVVVSFISDESSLSNAGSKLQDVL 60

Query: 61  RETNEKMPSTLRDKAAFTLLGRYDISRNANGNVSKIIRDKDMSRKAGAYVRPCKYSGLSC 120
           RETNEKMPSTLRDKAAFTLLGRYDISRNANGNVSKIIRDKDMSRKAGAYVRPCKYSGLSC
Sbjct: 61  RETNEKMPSTLRDKAAFTLLGRYDISRNANGNVSKIIRDKDMSRKAGAYVRPCKYSGLSC 120

Query: 121 GCHK---------------------------SIELWNSIQVLDCIIYTGMGILYLNAMST 153
           GCHK                            I  ++S   L  I + G     ++    
Sbjct: 121 GCHKVDGLLEDFRKDSVRNYWIHMVHDSAEHRIMEFHSSSRLHHIHWNGD---IVSQCDV 177

Query: 154 YVIVYDTPVFGSHHFSLSFSNSSPQTKAPLKKPKWVDDLHNRKPLNEMETVILSLNCAAA 213
           +VIVYDTPVFGSHHFSLSFSNSSPQTKAPLKKPKWVDDLHNRKPLNEMETVILSLNCAAA
Sbjct: 178 HVIVYDTPVFGSHHFSLSFSNSSPQTKAPLKKPKWVDDLHNRKPLNEMETVILSLNCAAA 237

Query: 214 AKIAYKKISTQLETSSQNFSISYL 237
           AKIAYKKISTQLETSSQNFSISYL
Sbjct: 238 AKIAYKKISTQLETSSQNFSISYL 261
>pir||T45795 hypothetical protein F28O9.20 - Arabidopsis thaliana
          Length = 242

 Score =  416 bits (1058), Expect = e-115
 Identities = 190/242 (78%), Positives = 196/242 (80%), Gaps = 30/242 (12%)

Query: 1   MKRKCRIWLPKQLASTDDLSHSLLFGWFVCHSSSCLDVVVSFISDESSLSNAGSKLQDVL 60
           MKRKCRIWLPKQLASTDDLSHSLLFGWFVCHSSSCLDVVVSFISDESSLSNAGSKLQDVL
Sbjct: 1   MKRKCRIWLPKQLASTDDLSHSLLFGWFVCHSSSCLDVVVSFISDESSLSNAGSKLQDVL 60

Query: 61  RETNEKMPSTLRDKAAFTLLGRYDISRNANGNVSKIIRDKDMSRKAGAYVRPCKYSGLSC 120
           RETNEKMPSTLRDKAAFTLLGRYDISRNANGNVSKIIRDKDMSRKAGAYVRPCKYSGLSC
Sbjct: 61  RETNEKMPSTLRDKAAFTLLGRYDISRNANGNVSKIIRDKDMSRKAGAYVRPCKYSGLSC 120

Query: 121 GCHK---------------------------SIELWNSIQVLDCIIYTGMGILYLNAMST 153
           GCHK                            I  ++S   L  I + G     ++    
Sbjct: 121 GCHKVDGLLEDFRKDSVRNYWIHMVHDSAEHRIMEFHSSSRLHHIHWNGD---IVSQCDV 177

Query: 154 YVIVYDTPVFGSHHFSLSFSNSSPQTKAPLKKPKWVDDLHNRKPLNEMETVILSLNCAAA 213
           +VIVYDTPVFGSHHFSLSFSNSSPQTKAPLKKPKWVDDLHNRKPLNEMETVILSLNCAAA
Sbjct: 178 HVIVYDTPVFGSHHFSLSFSNSSPQTKAPLKKPKWVDDLHNRKPLNEMETVILSLNCAAA 237

Query: 214 AK 215
           AK
Sbjct: 238 AK 239
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
           component)) [Homo sapiens]
          Length = 583

 Score =  396 bits (1007), Expect = e-109
 Identities = 100/346 (28%), Positives = 163/346 (46%), Gaps = 23/346 (6%)

Query: 266 LLSSFLIFSWVHIASRRVLKNTW----INFRIRSCQILYWPIFLEEIDMMSISCVKHAEE 321
           LL+S L       A RR +   W    +  ++ +C+ L     LE + +  I   + AE 
Sbjct: 215 LLASLLSLVSAVSACRRRVFKLWPLSFLGSKLSTCEQLRHR--LEHLTL--IFSTRKAEN 270

Query: 322 AA--LQRHSTWSAMAVDLVLGNLI-----GLGLLFNTESVCSFVFDFAKEFTNGILRSGS 374
            A  +++ +T +++ +D+ LG ++     G   + +       V D   E    +L+   
Sbjct: 271 PAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHLLQ--- 327

Query: 375 VWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATV 434
            WLMG PAG K+N  L  VLG   L  I +W +    M+ F+  ++  +  L    G TV
Sbjct: 328 -WLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG-LSACLGLTV 385

Query: 435 SAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTV 494
           + + + D+I   T HI   +     +Y  +I  L++LWRLFRG+K N LRQR+DS  Y +
Sbjct: 386 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 445

Query: 495 KQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWL 554
            Q  +G+LLFT LL LLPTT+++Y+ FT+    + ++  LI   + +I++ P   + + L
Sbjct: 446 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 505

Query: 555 VRRKRFPCGVWFEMEHCGEHILKSNDAFEDSKSLLEEHGTPEKNSL 600
            R  R   GV F +     H            + L          L
Sbjct: 506 CRPYRLAAGVKFRVLR---HEAGRPLRLLMQINPLPYSRVVHTYRL 548
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
           N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
          Length = 581

 Score =  387 bits (986), Expect = e-106
 Identities = 99/346 (28%), Positives = 162/346 (46%), Gaps = 25/346 (7%)

Query: 266 LLSSFLIFSWVHIASRRVLKNTW----INFRIRSCQILYWPIFLEEIDMMSISCVKHAEE 321
           LL+S L       A R  +   W    +  ++ +C+ L     LE + +  I   + AE 
Sbjct: 215 LLASLLSLVSAVSACR--VFKLWPLSFLGSKLSTCEQLRHR--LEHLTL--IFSTRKAEN 268

Query: 322 AA--LQRHSTWSAMAVDLVLGNLI-----GLGLLFNTESVCSFVFDFAKEFTNGILRSGS 374
            A  +++ +T +++ +D+ LG ++     G   + +       V D   E    +L+   
Sbjct: 269 PAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHLLQ--- 325

Query: 375 VWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATV 434
            WLMG PAG K+N  L  VLG   L  I +W +    M+ F+  ++  +  L    G TV
Sbjct: 326 -WLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG-LSACLGLTV 383

Query: 435 SAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTV 494
           + + + D+I   T HI   +     +Y  +I  L++LWRLFRG+K N LRQR+DS  Y +
Sbjct: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443

Query: 495 KQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWL 554
            Q  +G+LLFT LL LLPTT+++Y+ FT+    + ++  LI   + +I++ P   + + L
Sbjct: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503

Query: 555 VRRKRFPCGVWFEMEHCGEHILKSNDAFEDSKSLLEEHGTPEKNSL 600
            R  R   GV F +     H            + L          L
Sbjct: 504 CRPYRLAAGVKFRVLR---HEASRPLRLLMQINPLPYSRVVHTYRL 546
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
           sapiens]
          Length = 581

 Score =  387 bits (986), Expect = e-106
 Identities = 99/346 (28%), Positives = 162/346 (46%), Gaps = 25/346 (7%)

Query: 266 LLSSFLIFSWVHIASRRVLKNTW----INFRIRSCQILYWPIFLEEIDMMSISCVKHAEE 321
           LL+S L       A R  +   W    +  ++ +C+ L     LE + +  I   + AE 
Sbjct: 215 LLASLLSLVSAVSACR--VFKLWPLSFLGSKLSTCEQLRHR--LEHLTL--IFSTRKAEN 268

Query: 322 AA--LQRHSTWSAMAVDLVLGNLI-----GLGLLFNTESVCSFVFDFAKEFTNGILRSGS 374
            A  +++ +T +++ +D+ LG ++     G   + +       V D   E    +L+   
Sbjct: 269 PAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHLLQ--- 325

Query: 375 VWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATV 434
            WLMG PAG K+N  L  VLG   L  I +W +    M+ F+  ++  +  L    G TV
Sbjct: 326 -WLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG-LSACLGLTV 383

Query: 435 SAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTV 494
           + + + D+I   T HI   +     +Y  +I  L++LWRLFRG+K N LRQR+DS  Y +
Sbjct: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443

Query: 495 KQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWL 554
            Q  +G+LLFT LL LLPTT+++Y+ FT+    + ++  LI   + +I++ P   + + L
Sbjct: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503

Query: 555 VRRKRFPCGVWFEMEHCGEHILKSNDAFEDSKSLLEEHGTPEKNSL 600
            R  R   GV F +     H            + L          L
Sbjct: 504 CRPYRLAAGVKFRVLR---HEAGRPLRLLMQINPLPYSRVVHTYRL 546
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
 gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 760

 Score =  386 bits (983), Expect = e-106
 Identities = 93/307 (30%), Positives = 154/307 (49%), Gaps = 22/307 (7%)

Query: 266 LLSSFLIFSWVHIASRRVLKNTW----INFRIRSCQILYWPIFLEEIDMMSISCVKHAEE 321
           LL+S L       A R  +   W    +  ++ +C+ L     LE + +  I   + AE 
Sbjct: 215 LLASLLSLVSAVSACR--VFKLWPLSFLGSKLSTCEQLRHR--LEHLTL--IFSTRKAEN 268

Query: 322 AA--LQRHSTWSAMAVDLVLGNLI-----GLGLLFNTESVCSFVFDFAKEFTNGILRSGS 374
            A  +++ +T +++ +D+ LG ++     G   + +       V D   E    +L+   
Sbjct: 269 PAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHLLQ--- 325

Query: 375 VWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATV 434
            WLMG PAG K+N  L  VLG   L  I +W +    M+ F+  ++  +  L    G TV
Sbjct: 326 -WLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG-LSACLGLTV 383

Query: 435 SAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTV 494
           + + + D+I   T HI   +     +Y  +I  L++LWRLFRG+K N LRQR+DS  Y +
Sbjct: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443

Query: 495 KQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWL 554
            Q  +G+LLFT LL LLPTT+++Y+ FT+    + ++  LI   + +I++ P   + + L
Sbjct: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503

Query: 555 VRRKRFP 561
            R  R  
Sbjct: 504 CRPYRLA 510
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
 gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
           (human) [Mus musculus]
          Length = 581

 Score =  360 bits (916), Expect = 4e-98
 Identities = 94/316 (29%), Positives = 153/316 (47%), Gaps = 22/316 (6%)

Query: 266 LLSSFLIFSWVHIASRRVLKNTW----INFRIRSCQILYWPIFLEEIDMMSISCVKHAEE 321
           LL+S L       A R  L   W    I  ++ +C+ L+    L+ +    I   + A+ 
Sbjct: 215 LLASLLSLISAASACR--LWKLWPLSFIRSKLSTCEQLHHR--LKHLSF--IFSTEKAQN 268

Query: 322 AA--LQRHSTWSAMAVDLVLGNLIGLG-----LLFNTESVCSFVFDFAKEFTNGILRSGS 374
               +++ +   ++ +D+ LG L+         +    +    V D   E    +L+   
Sbjct: 269 PMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQ--- 325

Query: 375 VWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATV 434
            WLMG PAG K+N  L  VLG   L  I +W +    M+ FI  ++  +  L    G TV
Sbjct: 326 -WLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVG-LSACLGLTV 383

Query: 435 SAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTV 494
           + +   D+I   T HI   +     +Y  +I  L++LWRLFRG+K N LRQR+DS  Y +
Sbjct: 384 ALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443

Query: 495 KQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWL 554
            Q  +G+LLFT L+ LLPTT+++Y+ FT+    + ++  LI   + +I++ P   + + L
Sbjct: 444 DQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRL 503

Query: 555 VRRKRFPCGVWFEMEH 570
            R  R   GV F +  
Sbjct: 504 CRPYRLAAGVKFRVLE 519
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
           musculus]
          Length = 581

 Score =  357 bits (907), Expect = 4e-97
 Identities = 93/316 (29%), Positives = 152/316 (47%), Gaps = 22/316 (6%)

Query: 266 LLSSFLIFSWVHIASRRVLKNT----WINFRIRSCQILYWPIFLEEIDMMSISCVKHAEE 321
           LL+S L       A R  L       +I  ++ +C+ L     L+ +    I   + A+ 
Sbjct: 215 LLASLLSLISAASACR--LWKLGPLAFIRSKLSTCEQLQHR--LKHLSF--IFSTEKAQS 268

Query: 322 AA--LQRHSTWSAMAVDLVLGNLIGLG-----LLFNTESVCSFVFDFAKEFTNGILRSGS 374
               +++ +   ++ +D+ LG L+         +    +    V D   E    +L+   
Sbjct: 269 PMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQ--- 325

Query: 375 VWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATV 434
            WLMG PAG K+N  L  VLG   L  I +W +    M+ FI  ++  +  L    G TV
Sbjct: 326 -WLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVG-LSACLGLTV 383

Query: 435 SAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTV 494
           + +   D+I   T HI   +     +Y  +I  L++LWRLFRG+K N LRQR+DS  Y +
Sbjct: 384 ALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443

Query: 495 KQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWL 554
            Q  +G+LLFT L+ LLPTT+++Y+ FT+    + ++  LI   + +I++ P   + + L
Sbjct: 444 DQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRL 503

Query: 555 VRRKRFPCGVWFEMEH 570
            R  R   GV F +  
Sbjct: 504 CRPYRLAAGVKFRVLE 519
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
           glycosylphosphatidylinositol homolog (S. cerevisiae)
           [Mus musculus]
 dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
          Length = 581

 Score =  356 bits (906), Expect = 6e-97
 Identities = 93/316 (29%), Positives = 152/316 (47%), Gaps = 22/316 (6%)

Query: 266 LLSSFLIFSWVHIASRRVLKNTW----INFRIRSCQILYWPIFLEEIDMMSISCVKHAEE 321
           LL+S L       A R  L   W    I  ++ +C+ L+    L+ +    I   + A+ 
Sbjct: 215 LLASLLSLISAASACR--LWKLWPLSFIRSKLSTCEQLHHR--LKHLSF--IFSTEKAQN 268

Query: 322 AA--LQRHSTWSAMAVDLVLGNLIGLG-----LLFNTESVCSFVFDFAKEFTNGILRSGS 374
               +++ +   ++ +D+ LG L+         +    +    V D   E    +L+   
Sbjct: 269 PMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQ--- 325

Query: 375 VWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATV 434
            WLMG PAG K+N  L  VLG   L  I +W +    M+ FI  ++  +  L    G TV
Sbjct: 326 -WLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVG-LSACLGLTV 383

Query: 435 SAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTV 494
           + +   D+I   T HI   +     +Y  +I  L++LWRLFRG+K N LRQR+DS  Y +
Sbjct: 384 ALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443

Query: 495 KQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWL 554
            Q  +G+LLFT L+ LLPTT+++ + FT+    + ++  LI   + +I++ P   + + L
Sbjct: 444 DQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRL 503

Query: 555 VRRKRFPCGVWFEMEH 570
            R  R   GV F +  
Sbjct: 504 CRPYRLAAGVKFRVLE 519
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi1p
           [Saccharomyces cerevisiae]
 sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
 gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
          Length = 609

 Score =  348 bits (884), Expect = 2e-94
 Identities = 101/464 (21%), Positives = 194/464 (41%), Gaps = 49/464 (10%)

Query: 184 KKPKWVDDLHNRKPLNEMETVILSLNCAAAAKIAYKKISTQLETSSQNFSISYLISSLTW 243
           + P++  D    K L+E   +I   N   A   A  ++   ++TS +N   + L S   W
Sbjct: 121 QHPRYKRD---NKKLSETLNII---NLFPAYCKALNELYPFIQTSQENLRGTMLNSVAAW 174

Query: 244 ----RLLATILGSLSSLYYSLAQFFYLLSSFLIFSWVHIAS-RRVLKNTWINFRIRSCQI 298
                +   +      L + +     L+SS L  ++ H            I+  +R  QI
Sbjct: 175 CSSTCIYKMVAKIGFYLTFVICSIASLVSSLL--NYSHFQLVNYSAFVQQID--LRCQQI 230

Query: 299 LYWPIFLEEI--------------------DMMSISCVKHAEEAALQRHSTWSAMAVDLV 338
            Y+P+  E I                                   +  ++T   +  D+ 
Sbjct: 231 CYFPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDIS 290

Query: 339 LGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVS 398
            G ++G  L+ N + + S      K F    L++ +  L   P G KLN ELA  L  + 
Sbjct: 291 FGLILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELF 350

Query: 399 LNVIQIWSTLWV-------FMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIM 451
           L VI+   T ++        ++S +   I ++ ++G +F  +++    ID     +  I 
Sbjct: 351 LWVIEFSYTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLA----IDFFAILSFPIY 406

Query: 452 ALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLL 511
             +   + +Y  Q+  +A+L+ LF G+K N LR R+D   + + Q ++G+LLF  L+ L 
Sbjct: 407 VFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLT 466

Query: 512 PTTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWLVRRKRFPCGVWFEMEHC 571
           PT   FY+ +T+      +I +  E  I++I+  P   +++ L   KR P G+  E++  
Sbjct: 467 PTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISIELK-- 524

Query: 572 GEHILKSNDAFEDSKSLLEEHGTPEKNSLMVSNLRSNFLTLGQI 615
              +   +   E   + ++        +L++S +R+N+ +   +
Sbjct: 525 -TTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFATV 567
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
          Length = 339

 Score =  347 bits (881), Expect = 5e-94
 Identities = 85/286 (29%), Positives = 143/286 (49%), Gaps = 16/286 (5%)

Query: 292 RIRSCQILYWPIFLEEIDMMSISCVKHAEEAA--LQRHSTWSAMAVDLVLGNLIGLG--- 346
           ++ +C+ L+    L+ +    I   + A+     +++ +   ++ +D+ LG L+      
Sbjct: 1   KLSTCEQLHHR--LKHLSF--IFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHS 56

Query: 347 --LLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQI 404
              +    +    V D   E    +L+    WLMG PAG K+N  L  VLG   L  I +
Sbjct: 57  NNRIGQLANALVPVADRVAEELQHLLQ----WLMGAPAGLKMNRALDQVLGRFFLYHIHL 112

Query: 405 WSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQ 464
           W +    M+ FI  ++  +  L    G TV+ +   D+I   T HI   +     +Y  +
Sbjct: 113 WISYIHLMSPFIEHILWHVG-LSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLK 171

Query: 465 IQALAALWRLFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTIT 524
           I  L++LWRLFRG+K N LRQR+DS  Y + Q  +G+LLFT L+ LLPTT+++Y+ FT+ 
Sbjct: 172 IYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLL 231

Query: 525 STTINSICMLIEFAISVIHATPYAEVMIWLVRRKRFPCGVWFEMEH 570
              + ++  LI   + +I++ P   + + L R  R   GV F +  
Sbjct: 232 RLLVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLE 277
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
          Length = 473

 Score =  279 bits (707), Expect = 1e-73
 Identities = 68/315 (21%), Positives = 128/315 (40%), Gaps = 28/315 (8%)

Query: 264 FYLLSSFLIFSWVHIASRRVLKNTWINFRIRSCQILYWPI----------FLEEIDMMSI 313
           FY+L+  +  +      R+   + W       C +   P+          F+  I +   
Sbjct: 138 FYMLAQLVQPN--PDTCRKS-NDLWFYGSQSLCLLANIPMQMFQYIVGNKFINSI-ITHT 193

Query: 314 SCVKHAEEAALQRHSTWSAMAVDLVLGNLIGLGLLFNTESVCSFVFDFA---KEFTNGIL 370
              +H +E   Q   T  +   ++++   +G+ L+    ++ S   DF           L
Sbjct: 194 VIYRHYKE--WQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFLIQISHIIIHQL 251

Query: 371 RSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITF 430
            S    L G P G KLN  L           I++WST    +   +  +   I   G   
Sbjct: 252 YSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFG-CL 310

Query: 431 GATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSY 490
           G T   A + D+I+   LH    +    ++ + +++ L  LW++ RG + N LR R++++
Sbjct: 311 GFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAH 370

Query: 491 GYTVKQHVVGSLLFTPLLLLLPTTSVFYIFF-TITSTTINSICMLIEFAISVIHATPYAE 549
            Y  +Q  + ++ F+ +L L PTT V+Y+ F T+ + T  ++  L  F   +++  P   
Sbjct: 371 NYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLN-FPIEM 429

Query: 550 VMIWLVRRKRFPCGV 564
            +      K    G 
Sbjct: 430 FL------KYIKKGF 438
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
          Length = 481

 Score =  277 bits (702), Expect = 5e-73
 Identities = 64/257 (24%), Positives = 115/257 (43%), Gaps = 7/257 (2%)

Query: 303 IFLEEIDMMSISCVKHAEEAALQRHSTWSAMAVDLVLGNLIGLGLLFNTESVCSFVFDFA 362
           +F+  I M+  +  KH +E   Q      +   ++VL  ++G+ L+    S+ +   DF 
Sbjct: 191 VFINSI-MVHTTIYKHFKE--WQTACDKRSRPANIVLDRILGIILMLILFSLATQPGDFL 247

Query: 363 ---KEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCL 419
                +    L      L G P G KLN  L           I++WST   F+   +  +
Sbjct: 248 IQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQV 307

Query: 420 IRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRK 479
              I ++G   G T   A ++D+I+   LH    +    ++Y+ + + L+ LW++ RG +
Sbjct: 308 FLAIGMIG-CLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNR 366

Query: 480 LNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAI 539
            N L+ R +S+ Y  +Q  + ++ F+ +L LLPTT V+YI F        +   +  F  
Sbjct: 367 YNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVR 426

Query: 540 SVIHATPYAEVMIWLVR 556
             +   P    +  L+R
Sbjct: 427 RKLMYLPIEVCIKRLLR 443
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
          Length = 669

 Score =  269 bits (682), Expect = 9e-71
 Identities = 82/343 (23%), Positives = 149/343 (42%), Gaps = 23/343 (6%)

Query: 252 SLSSLYYSLAQFFYLLSSFLIFSWVHIASRRVLKNTWINFRIRSC----QILYWPIFLEE 307
           S+ S   S+  FF  + S +     +I     L + +I  +   C    Q   +P F+  
Sbjct: 272 SIFSYIVSIILFFTYIISLINSCLYYIICTPKLFSEYIFSK--KCDGYLQNSAYPKFIFP 329

Query: 308 IDMMSIS-CVKHAEEAALQRHS-------TWSAMAVDLVLGNLIGLGLLFNTESVCSFVF 359
            +  +I       ++   + +            + +D+ LG LI L L FN  ++  ++ 
Sbjct: 330 SEWHNIFRSFMKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINL-HYIS 388

Query: 360 DFAKEF-TNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMA---SF 415
           + A+ F     L S    L+  P GFKLN      +G + ++++  W      +    S 
Sbjct: 389 EKAQIFYGTSTLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNST 448

Query: 416 IFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLF 475
           +   +   ++LG +F      +FVID + F T H+  ++  +  + +   + + +L+ LF
Sbjct: 449 VLNFVGYTSLLGFSF----FLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLF 504

Query: 476 RGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLI 535
            G+K N L+ R+D+  Y+ ++ ++G++LFT L+ L PT  V  + F +    IN I  L+
Sbjct: 505 NGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLL 564

Query: 536 EFAISVIHATPYAEVMIWLVRRKRFPCGVWFEMEHCGEHILKS 578
                +I  TP+    I     K    G  F     GEH L  
Sbjct: 565 CVMEKIILYTPFYIFFIQPNCNKYISKGFKFTKYEVGEHELLK 607
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
 pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
 emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
          Length = 287

 Score =  214 bits (541), Expect = 4e-54
 Identities = 55/235 (23%), Positives = 89/235 (37%), Gaps = 18/235 (7%)

Query: 319 AEEAALQRHSTWSAMAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLM 378
           A+   ++  S    + +D+     +      N E   +  +D         L     WL 
Sbjct: 16  AKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVA-DNLNGTITWLR 74

Query: 379 GVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFC----------------LIRV 422
             PAG KLNT +   L       I +W+++      F F                   R 
Sbjct: 75  SNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGFLRSDAFFRF 134

Query: 423 IAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNP 482
           IA   +  G +  +A V D      LH        T +       L  LW L RG+K NP
Sbjct: 135 IA-YSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNP 193

Query: 483 LRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEF 537
           LR+R D+     +Q  + + LF  LL +LPT  V+++ F      ++++  ++ F
Sbjct: 194 LRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYF 248
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana  (fragment)
 emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
          Length = 143

 Score =  169 bits (425), Expect = 1e-40
 Identities = 143/143 (100%), Positives = 143/143 (100%)

Query: 333 MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 392
           MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG
Sbjct: 1   MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 60

Query: 393 VLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMA 452
           VLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMA
Sbjct: 61  VLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMA 120

Query: 453 LHWAITLVYSHQIQALAALWRLF 475
           LHWAITLVYSHQIQALAALWRLF
Sbjct: 121 LHWAITLVYSHQIQALAALWRLF 143
>gb|AAH10094.1|AAH10094 (BC010094) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 299

 Score = 43.6 bits (102), Expect = 0.010
 Identities = 15/62 (24%), Positives = 25/62 (40%), Gaps = 10/62 (16%)

Query: 266 LLSSFLIFSWVHIASRRVLKNTW----INFRIRSCQILYWPIFLEEIDMMSISCVKHAEE 321
           LL+S L       A R  +   W    +  ++ +C+ L     LE + +  I   + AE 
Sbjct: 215 LLASLLSLVSAVSACR--VFKLWPLSFLGSKLSTCEQLRHR--LEHLTL--IFSTRKAEN 268

Query: 322 AA 323
            A
Sbjct: 269 PA 270
>dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:1333114,
           evidence:ISS~phosphatidylinositol glycan, class
           Q~putative [Mus musculus]
          Length = 378

 Score = 35.5 bits (81), Expect = 2.7
 Identities = 20/107 (18%), Positives = 41/107 (37%), Gaps = 17/107 (15%)

Query: 266 LLSSFLIFSWVHIASRRVLKNTW----INFRIRSCQILYWPIFLEEIDMMSISCVKHAEE 321
           LL+S L       A R  L   W    I  ++ +C+ L+    L+ +    I   + A+ 
Sbjct: 215 LLASLLSLISAASACR--LWKLWPLSFIRSKLSTCEQLHHR--LKHLSF--IFSTEKAQN 268

Query: 322 AA--LQRHSTWSAMAVDLVLGNLIGLG-----LLFNTESVCSFVFDF 361
               +++ +   ++ +D+ LG L+         +    +    V D 
Sbjct: 269 PMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADV 315
CPU time:   158.45 user secs.	    3.14 sys. secs	  161.59 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.321    0.159    0.484 

Gapped
Lambda     K      H
   0.270   0.0559    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 389592965
Number of Sequences: 887402
Number of extensions: 17083079
Number of successful extensions: 74990
Number of sequences better than 10.0: 134
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 115
Number of HSP's that attempted gapping in prelim test: 74689
Number of HSP's gapped (non-prelim): 252
length of query: 680
length of database: 277,845,442
effective HSP length: 55
effective length of query: 625
effective length of database: 229,038,332
effective search space: 143148957500
effective search space used: 143148957500
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.2 bits)
S2: 76 (33.8 bits)