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GPI Anchor Biosynthesis Report: NP_011732.1 (PIG-Q family, Saccharomyces cerevisiae)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (609 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 4

Distribution of 21 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 803 0.0
sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 598 e-170
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 569 e-161
ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 468 e-131
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 458 e-127
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 458 e-127
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 456 e-127
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 455 e-127
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 452 e-126
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 450 e-125
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 428 e-118
emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 405 e-111
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 375 e-102
emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 362 7e-99
emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 352 8e-96
ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 269 7e-71
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 155 2e-36
emb|CAB68123.2| (AL137080) hypothetical protein [Arabidopsis tha... 116 8e-25
pir||T45795 hypothetical protein F28O9.20 - Arabidopsis thaliana 110 8e-23
dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:13331... 64 6e-09
gb|AAH10094.1|AAH10094 (BC010094) Similar to phosphatidylinosito... 51 8e-05
Alignments
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi1p
           [Saccharomyces cerevisiae]
 sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
 gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
          Length = 609

 Score =  803 bits (2052), Expect = 0.0
 Identities = 609/609 (100%), Positives = 609/609 (100%)

Query: 1   MPNYIFWPYESLFENSAAQGPQVALAISFEKTHFVVLGVCEPQYLEEVSIRPPYSVVATK 60
           MPNYIFWPYESLFENSAAQGPQVALAISFEKTHFVVLGVCEPQYLEEVSIRPPYSVVATK
Sbjct: 1   MPNYIFWPYESLFENSAAQGPQVALAISFEKTHFVVLGVCEPQYLEEVSIRPPYSVVATK 60

Query: 61  NNGAEGWNYKVADPCNVHFRIPKLKFMQFYSSDPISLIIPEKEVGLHSSVGETLNYSKLE 120
           NNGAEGWNYKVADPCNVHFRIPKLKFMQFYSSDPISLIIPEKEVGLHSSVGETLNYSKLE
Sbjct: 61  NNGAEGWNYKVADPCNVHFRIPKLKFMQFYSSDPISLIIPEKEVGLHSSVGETLNYSKLE 120

Query: 121 QHPRYKRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQENLRGTMLNSVAAWCSSTCI 180
           QHPRYKRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQENLRGTMLNSVAAWCSSTCI
Sbjct: 121 QHPRYKRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQENLRGTMLNSVAAWCSSTCI 180

Query: 181 YKMVAKIGFYLTFVICSIASLVSSLLNYSHFQLVNYSAFVQQIDLRCQQICYFPVQYERI 240
           YKMVAKIGFYLTFVICSIASLVSSLLNYSHFQLVNYSAFVQQIDLRCQQICYFPVQYERI
Sbjct: 181 YKMVAKIGFYLTFVICSIASLVSSLLNYSHFQLVNYSAFVQQIDLRCQQICYFPVQYERI 240

Query: 241 NKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGLILGAILI 300
           NKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGLILGAILI
Sbjct: 241 NKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGLILGAILI 300

Query: 301 ENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSYTT 360
           ENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSYTT
Sbjct: 301 ENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSYTT 360

Query: 361 FIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQL 420
           FIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQL
Sbjct: 361 FIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQL 420

Query: 421 NIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLR 480
           NIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLR
Sbjct: 421 NIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLR 480

Query: 481 MLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISIELKTTVSNKHTTLELQNNP 540
           MLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISIELKTTVSNKHTTLELQNNP
Sbjct: 481 MLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISIELKTTVSNKHTTLELQNNP 540

Query: 541 IKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESIMVNRNKLYYVLYSSLPSKPLSVKD 600
           IKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESIMVNRNKLYYVLYSSLPSKPLSVKD
Sbjct: 541 IKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESIMVNRNKLYYVLYSSLPSKPLSVKD 600

Query: 601 LYKRLTIQA 609
           LYKRLTIQA
Sbjct: 601 LYKRLTIQA 609
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
           - fission yeast (Schizosaccharomyces pombe)
 emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
           protein gpi1. [Schizosaccharomyces pombe]
          Length = 653

 Score =  598 bits (1527), Expect = e-170
 Identities = 158/553 (28%), Positives = 283/553 (50%), Gaps = 67/553 (12%)

Query: 77  VHFRIPKLKFMQFYSSDP----------ISLIIPEKEVGLHSSVGETLNYSKLEQH-PRY 125
           +++  P+ + MQF S +P          I+   PE E   H    + +   KL+ H PR 
Sbjct: 127 IYYHPPQPRKMQFLSLEPLSLLLLKDSFINKSNPEYESMQH----QQILLKKLKLHFPRR 182

Query: 126 KRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQE--NLRGTMLNSVAAWCSSTCIYKM 183
           K ++ K S    +I L       LN+ +     + E  N + + +  +    SS+  Y  
Sbjct: 183 KENSWKRSLRSGLIEL-------LNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFF 235

Query: 184 VAKIGFYLTFVICSIASLVSSLLNYS----HFQLVNYSAFVQQIDLRCQQICYFPVQYER 239
            +   +++  ++  I  ++   +NY      + +++     +Q+DLR QQ C++PVQY +
Sbjct: 236 NSLFAYFI-ILLRIINEVILLAINYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMK 294

Query: 240 I-----NKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGLI 294
           +     +K+  I++                       Y +YI  YN +WL+ ND+ FG+ 
Sbjct: 295 LWVFRKSKRVAIED-----------------------YKEYIRFYNNLWLVANDMIFGIT 331

Query: 295 LGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVI 354
           + + ++EN   +V     +   +   +++++   L + P G+KLN ++  F+ +L +WVI
Sbjct: 332 MSSFILENLHLVVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVI 391

Query: 355 EFSYTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSK 414
           +  ++ F+   +     +  L   + +    G S  ++L  DF ++++  I++ Y  SS+
Sbjct: 392 DV-WSNFLLHCL---PWTPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSR 447

Query: 415 LYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYM 474
           LY+ QL ++ SL  LF GKKRNVLRNRID   + LDQLLLGT+LF +L+F  PT+  FY 
Sbjct: 448 LYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYA 507

Query: 475 SYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISIELKTTVSNKHT-- 532
           ++ + R+  +T     E ++A +NHFPLF  +LR+KDP R+P G++ E+ +    K    
Sbjct: 508 AFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEIVSFEPLKQDGF 567

Query: 533 -TLELQNN--PIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESI-MVNRNKLYYVLY 588
            TL L  N  P+   SMF  Y  L  ++ ++Y S  T+  ++ G  +  +   +LY + Y
Sbjct: 568 ATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKTTLISLLVGCPVPAIPAEQLYNIQY 627

Query: 589 SSLPSKPLSVKDL 601
           + LP+K +S++ L
Sbjct: 628 AMLPTKRISIRKL 640
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
          Length = 517

 Score =  569 bits (1453), Expect = e-161
 Identities = 156/543 (28%), Positives = 277/543 (50%), Gaps = 67/543 (12%)

Query: 87  MQFYSSDP----------ISLIIPEKEVGLHSSVGETLNYSKLEQH-PRYKRDNKKLSET 135
           MQF S +P          I+   PE E   H    + +   KL+ H PR K ++ K S  
Sbjct: 1   MQFLSLEPLSLLLLKDSFINKSNPEYESMQH----QQILLKKLKLHFPRRKENSWKRSLR 56

Query: 136 LNIINLFPAYCKALNELYPFIQTSQE--NLRGTMLNSVAAWCSSTCIYKMVAKIGFYLTF 193
             +I L       LN+ +     + E  N + + +  +    SS+  Y   +   +++  
Sbjct: 57  SGLIEL-------LNQSFEVRMLTHENNNKKNSYVFRLFDRVSSSTFYFFNSLFAYFI-I 108

Query: 194 VICSIASLVSSLLNYS----HFQLVNYSAFVQQIDLRCQQICYFPVQYERI-----NKKD 244
           ++  I  ++   +NY      + +++     +Q+DLR QQ C++PVQY ++     +K+ 
Sbjct: 109 LLRIINEVILLAINYRPIPLSYNMMDIFVSARQVDLRLQQACFWPVQYMKLWVFRKSKRV 168

Query: 245 NIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGLILGAILIENRD 304
            I++                       Y +YI  YN +WL+ ND+ FG+ + + ++EN  
Sbjct: 169 AIED-----------------------YKEYIRFYNNLWLVANDMIFGITMSSFILENLH 205

Query: 305 FLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSYTTFIKR 364
            +V     +   +   +++++   L + P G+KLN ++  F+ +L +WVI+  ++ F+  
Sbjct: 206 LVVKLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDV-WSNFLLH 264

Query: 365 LIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQLNIMA 424
            +     +  L   + +    G S  ++L  DF ++++  I++ Y  SS++Y+ QL ++ 
Sbjct: 265 CL---PWTPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRMYNWQLRVIY 321

Query: 425 SLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTI 484
           SL  LF GKKRNVLRNRID   + LDQLLLGT+LF +L+F  PT+  FY ++ + R+  +
Sbjct: 322 SLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVM 381

Query: 485 TIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISIELKTTVSNKHT---TLELQNN-- 539
           T     E ++A +NHFPLF  +LR+KDP R+P G++ E+ +    K     TL L  N  
Sbjct: 382 TCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSK 441

Query: 540 PIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESI-MVNRNKLYYVLYSSLPSKPLSV 598
           P+   SMF  Y  L  ++ ++Y S  T+  ++ G  +  +   +LY + Y+ LP+K +S+
Sbjct: 442 PMSLGSMFEHYRKLARRLISHYLSKTTLISLLVGCPVPAIPAEQLYNIQYAMLPTKRISI 501

Query: 599 KDL 601
           + L
Sbjct: 502 RKL 504
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
 pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
 emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
          Length = 680

 Score =  468 bits (1194), Expect = e-131
 Identities = 101/512 (19%), Positives = 200/512 (38%), Gaps = 73/512 (14%)

Query: 77  VHFRIPKLKFMQFYSSDPISLIIPEKEVGLHSSVGETLNYSKLEQHPRYKRDNKKLSETL 136
           + +  P      F  S   S   P+ +  L             + H R   +  +     
Sbjct: 156 IVYDTPVFGSHHF--SLSFSNSSPQTKAPLKKPKWVD------DLHNRKPLNEMETVILS 207

Query: 137 NIINLFPAYCKALNELYPFIQTSQENLRGTMLNSVAAW--------------CSSTCIYK 182
             +N   A   A  ++   ++TS +N   + L S   W               S    + 
Sbjct: 208 --LNCAAAAKIAYKKISTQLETSSQNFSISYLISSLTWRLLATILGSLSSLYYSLAQFFY 265

Query: 183 MVAKIGFYLTFVICSIASLVSSLLNYSHFQLVNYSAFVQQIDLRCQQICYFPVQYERINK 242
           +++    +    I S   L ++ +N+                +R  QI Y+P+  E I  
Sbjct: 266 LLSSFLIFSWVHIASRRVLKNTWINFR---------------IRSCQILYWPIFLEEI-- 308

Query: 243 KDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGLILGAILIEN 302
             ++ ++  +   + +  Q   ++                  +  D+  G ++G  L+ N
Sbjct: 309 --DMMSISCVKHAEEAALQRHSTWSA----------------MAVDLVLGNLIGLGLLFN 350

Query: 303 RDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSYTTFI 362
            + + S      K F    L++ +  L   P G KLN ELA  L  + L VI+   T + 
Sbjct: 351 TESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLW- 409

Query: 363 KRLIDPKTLSSLLTLTIYMMFLVGFSFA----VSLAIDFFAILSFPIYVFYRISSKLYHC 418
                   ++S +   I ++ ++G +F      +  ID     +  I   +   + +Y  
Sbjct: 410 ------VFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSH 463

Query: 419 QLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTV 478
           Q+  +A+L+ LF G+K N LR R+D   + + Q ++G+LLF  L+ L PT   FY+ +T+
Sbjct: 464 QIQALAALWRLFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTI 523

Query: 479 LRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISIEL---KTTVSNKHTTLE 535
                 +I +  E  I++I+  P   +++ L   KR P G+  E+      +   +   E
Sbjct: 524 TSTTINSICMLIEFAISVIHATPYAEVMIWLVRRKRFPCGVWFEMEHCGEHILKSNDAFE 583

Query: 536 LQNNPIKFKSMFRPYNLLLSQMRTNYFSFATV 567
              + ++        +L++S +R+N+ +   +
Sbjct: 584 DSKSLLEEHGTPEKNSLMVSNLRSNFLTLGQI 615
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
           component)) [Homo sapiens]
          Length = 583

 Score =  458 bits (1167), Expect = e-127
 Identities = 103/356 (28%), Positives = 173/356 (47%), Gaps = 32/356 (8%)

Query: 233 FPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFG 292
           +P+ +   +K    + +   +E          +  P++     +   NT+  ++ D++ G
Sbjct: 237 WPLSFLG-SKLSTCEQLRHRLEHLTLIFSTRKAENPAQL----MRKANTVASVLLDVALG 291

Query: 293 LILGAILIENR------DFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFL 346
           L+L + L          D LV  +  V      + L+ + + L   P G+K+N  L   L
Sbjct: 292 LMLLSWLHGRSRIGHLADALVPVADHVA-----EELQHLLQWLMGAPAGLKMNRALDQVL 346

Query: 347 SELFLWVIEFS------YTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAI 400
              FL+ I          + F++ ++    LS+ L LT+          A+SL  D  A+
Sbjct: 347 GRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV----------ALSLLSDIIAL 396

Query: 401 LSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFI 460
           L+F IY FY   ++LY  +++ ++SL+ LF GKK NVLR R+D   + LDQL +GTLLF 
Sbjct: 397 LTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFT 456

Query: 461 ILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGIS 520
           IL+FL PT   +Y+ +T+LR+L + ++     ++ LIN  PL++L LRL  P RL  G+ 
Sbjct: 457 ILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVK 516

Query: 521 IELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESI 576
             +    + +   L +Q NP+ +  +   Y L        +   A  RK+  GE I
Sbjct: 517 FRVLRHEAGRPLRLLMQINPLPYSRVVHTYRLPSCGCHPKHSWGALCRKLFLGELI 572
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
           sapiens]
          Length = 581

 Score =  458 bits (1166), Expect = e-127
 Identities = 103/356 (28%), Positives = 173/356 (47%), Gaps = 32/356 (8%)

Query: 233 FPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFG 292
           +P+ +   +K    + +   +E          +  P++     +   NT+  ++ D++ G
Sbjct: 235 WPLSFLG-SKLSTCEQLRHRLEHLTLIFSTRKAENPAQL----MRKANTVASVLLDVALG 289

Query: 293 LILGAILIENR------DFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFL 346
           L+L + L          D LV  +  V      + L+ + + L   P G+K+N  L   L
Sbjct: 290 LMLLSWLHGRSRIGHLADALVPVADHVA-----EELQHLLQWLMGAPAGLKMNRALDQVL 344

Query: 347 SELFLWVIEFS------YTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAI 400
              FL+ I          + F++ ++    LS+ L LT+          A+SL  D  A+
Sbjct: 345 GRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV----------ALSLLSDIIAL 394

Query: 401 LSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFI 460
           L+F IY FY   ++LY  +++ ++SL+ LF GKK NVLR R+D   + LDQL +GTLLF 
Sbjct: 395 LTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFT 454

Query: 461 ILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGIS 520
           IL+FL PT   +Y+ +T+LR+L + ++     ++ LIN  PL++L LRL  P RL  G+ 
Sbjct: 455 ILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVK 514

Query: 521 IELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESI 576
             +    + +   L +Q NP+ +  +   Y L        +   A  RK+  GE I
Sbjct: 515 FRVLRHEAGRPLRLLMQINPLPYSRVVHTYRLPSCGCHPKHSWGALCRKLFLGELI 570
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
           N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
          Length = 581

 Score =  456 bits (1161), Expect = e-127
 Identities = 103/356 (28%), Positives = 174/356 (47%), Gaps = 32/356 (8%)

Query: 233 FPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFG 292
           +P+ +   +K    + +   +E          +  P++     +   NT+  ++ D++ G
Sbjct: 235 WPLSFLG-SKLSTCEQLRHRLEHLTLIFSTRKAENPAQL----MRKANTVASVLLDVALG 289

Query: 293 LILGAILIENR------DFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFL 346
           L+L + L          D LV  +  V      + L+ + + L   P G+K+N  L   L
Sbjct: 290 LMLLSWLHGRSRIGHLADALVPVADHVA-----EELQHLLQWLMGAPAGLKMNRALDQVL 344

Query: 347 SELFLWVIEFS------YTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAI 400
              FL+ I          + F++ ++    LS+ L LT+          A+SL  D  A+
Sbjct: 345 GRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV----------ALSLLSDIIAL 394

Query: 401 LSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFI 460
           L+F IY FY   ++LY  +++ ++SL+ LF GKK NVLR R+D   + LDQL +GTLLF 
Sbjct: 395 LTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFT 454

Query: 461 ILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGIS 520
           IL+FL PT   +Y+ +T+LR+L + ++     ++ LIN  PL++L LRL  P RL  G+ 
Sbjct: 455 ILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVK 514

Query: 521 IELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESI 576
             +    +++   L +Q NP+ +  +   Y L        +   A  RK+  GE I
Sbjct: 515 FRVLRHEASRPLRLLMQINPLPYSRVVHTYRLPSCGCHPKHSWGALCRKLFLGELI 570
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
 gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
           (human) [Mus musculus]
          Length = 581

 Score =  455 bits (1160), Expect = e-127
 Identities = 106/356 (29%), Positives = 175/356 (48%), Gaps = 32/356 (8%)

Query: 233 FPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFG 292
           +P+ + R +K    + +   ++  +       +  P +     +   N +  ++ D++ G
Sbjct: 235 WPLSFIR-SKLSTCEQLHHRLKHLSFIFSTEKAQNPMQL----MRKANMLVSVLLDVALG 289

Query: 293 LILGAILIENR------DFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFL 346
           L+L + L  N       + LV  + RV      + L+ + + L   P G+K+N  L   L
Sbjct: 290 LLLLSWLHSNNRIGQLANALVPVADRVA-----EELQHLLQWLMGAPAGLKMNRALDQVL 344

Query: 347 SELFLWVIEFS------YTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAI 400
              FL+ I          + FI+ ++    LS+ L LT+          A+S+  D  A+
Sbjct: 345 GRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTV----------ALSIFSDIIAL 394

Query: 401 LSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFI 460
           L+F IY FY   ++LY  ++  ++SL+ LF GKK NVLR R+D   + LDQL +GTLLF 
Sbjct: 395 LTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFT 454

Query: 461 ILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGIS 520
           ILVFL PT   +Y+ +T+LR+L IT++     ++ LIN  PL++L LRL  P RL  G+ 
Sbjct: 455 ILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVK 514

Query: 521 IELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESI 576
             +    + +   L +Q NP+ +  +   Y L        +      RK+V GE I
Sbjct: 515 FRVLEKEAGRPLRLLMQINPLSYNHVMHIYRLPRCGCHPKHSWGTLCRKLVFGELI 570
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
           glycosylphosphatidylinositol homolog (S. cerevisiae)
           [Mus musculus]
 dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
          Length = 581

 Score =  452 bits (1151), Expect = e-126
 Identities = 105/356 (29%), Positives = 174/356 (48%), Gaps = 32/356 (8%)

Query: 233 FPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFG 292
           +P+ + R +K    + +   ++  +       +  P +     +   N +  ++ D++ G
Sbjct: 235 WPLSFIR-SKLSTCEQLHHRLKHLSFIFSTEKAQNPMQL----MRKANMLVSVLLDVALG 289

Query: 293 LILGAILIENR------DFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFL 346
           L+L + L  N       + LV  + RV      + L+ + + L   P G+K+N  L   L
Sbjct: 290 LLLLSWLHSNNRIGQLANALVPVADRVA-----EELQHLLQWLMGAPAGLKMNRALDQVL 344

Query: 347 SELFLWVIEFS------YTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAI 400
              FL+ I          + FI+ ++    LS+ L LT+          A+S+  D  A+
Sbjct: 345 GRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTV----------ALSIFSDIIAL 394

Query: 401 LSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFI 460
           L+F IY FY   ++LY  ++  ++SL+ LF GKK NVLR R+D   + LDQL +GTLLF 
Sbjct: 395 LTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFT 454

Query: 461 ILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGIS 520
           ILVFL PT   + + +T+LR+L IT++     ++ LIN  PL++L LRL  P RL  G+ 
Sbjct: 455 ILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVK 514

Query: 521 IELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESI 576
             +    + +   L +Q NP+ +  +   Y L        +      RK+V GE I
Sbjct: 515 FRVLEKEAGRPLRLLMQINPLSYNHVMHIYRLPRCGCHPKHSWGTLCRKLVFGELI 570
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
           musculus]
          Length = 581

 Score =  450 bits (1148), Expect = e-125
 Identities = 105/355 (29%), Positives = 173/355 (48%), Gaps = 32/355 (9%)

Query: 234 PVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGL 293
           P+ + R +K    + +   ++  +       +  P +     +   N +  ++ D++ GL
Sbjct: 236 PLAFIR-SKLSTCEQLQHRLKHLSFIFSTEKAQSPMQL----MRKANMLVSVLLDVALGL 290

Query: 294 ILGAILIENR------DFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLS 347
           +L + L  N       + LV  + RV      + L+ + + L   P G+K+N  L   L 
Sbjct: 291 LLLSWLHSNNRIGQLANALVPVADRVA-----EELQHLLQWLMGAPAGLKMNRALDQVLG 345

Query: 348 ELFLWVIEFS------YTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAIL 401
             FL+ I          + FI+ ++    LS+ L LT+          A+S+  D  A+L
Sbjct: 346 RFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTV----------ALSIFSDIIALL 395

Query: 402 SFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFII 461
           +F IY FY   ++LY  ++  ++SL+ LF GKK NVLR R+D   + LDQL +GTLLF I
Sbjct: 396 TFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTI 455

Query: 462 LVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISI 521
           LVFL PT   +Y+ +T+LR+L IT++     ++ LIN  PL++L LRL  P RL  G+  
Sbjct: 456 LVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKF 515

Query: 522 ELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESI 576
            +    + +   L +Q NP+ +  +   Y L        +      RK+  GE I
Sbjct: 516 RVLEKEAGRPLRLLMQINPLPYSHVVHTYRLPSCGCHPKHSWGTLCRKLFFGELI 570
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
          Length = 339

 Score =  428 bits (1091), Expect = e-118
 Identities = 104/347 (29%), Positives = 169/347 (47%), Gaps = 31/347 (8%)

Query: 242 KKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFGLILGAILIE 301
           K    + +   ++  +       +  P +     +   N +  ++ D++ GL+L + L  
Sbjct: 1   KLSTCEQLHHRLKHLSFIFSTEKAQNPMQL----MRKANMLVSVLLDVALGLLLLSWLHS 56

Query: 302 NR------DFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIE 355
           N       + LV  + RV      + L+ + + L   P G+K+N  L   L   FL+ I 
Sbjct: 57  NNRIGQLANALVPVADRVA-----EELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIH 111

Query: 356 FS------YTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFY 409
                    + FI+ ++    LS+ L LT+          A+S+  D  A+L+F IY FY
Sbjct: 112 LWISYIHLMSPFIEHILWHVGLSACLGLTV----------ALSIFSDIIALLTFHIYCFY 161

Query: 410 RISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTV 469
              ++LY  ++  ++SL+ LF GKK NVLR R+D   + LDQL +GTLLF ILVFL PT 
Sbjct: 162 VYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTT 221

Query: 470 MAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLPGGISIELKTTVSN 529
             +Y+ +T+LR+L IT++     ++ LIN  PL++L LRL  P RL  G+   +    + 
Sbjct: 222 ALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAG 281

Query: 530 KHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFATVRKIVRGESI 576
           +   L +Q NP+ +  +   Y L        +      RK+V GE I
Sbjct: 282 RPLRLLMQINPLSYNHVMHIYRLPRCGCHPKHSWGTLCRKLVFGELI 328
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
          Length = 473

 Score =  405 bits (1031), Expect = e-111
 Identities = 84/380 (22%), Positives = 158/380 (41%), Gaps = 23/380 (6%)

Query: 141 LFPAYCKALNELYPFIQTSQENLRGTMLNSVAAWCSSTCIYKMVAKIGFYLTFVICSIAS 200
            F     A N    F   + ENL    L         + + K    +             
Sbjct: 77  CFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSLVIKDNNTLA-----HWTRENQ 131

Query: 201 LVSSLLNYSHFQLVNYSAFVQQIDLRCQQICYFPVQYERINKKDNIQNVGSMVEKDNSNS 260
           +      Y   QLV  +    +   +   + ++  Q   +     +Q    +V     NS
Sbjct: 132 INDECDFYMLAQLVQPNPDTCR---KSNDLWFYGSQSLCLLANIPMQMFQYIVGNKFINS 188

Query: 261 QFSHSYMPSKFYPDYILLY---NTIWLIINDISFGLILGAILIENRDFLVSASHRVL--- 314
             +H+ +  + Y ++  +Y   + +  I+ D + G+IL  +L      LVS     L   
Sbjct: 189 IITHTVI-YRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFT----LVSHPGDFLIQI 243

Query: 315 KFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSYTTFIKRLIDPKTLSSL 374
              +   L ++ + L  +P+G+KLN  L NF  + F + IE  ++TF+  LI+P      
Sbjct: 244 SHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIEL-WSTFLD-LIEPIVRQVF 301

Query: 375 LTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKK 434
           L +  +    +GF++ ++L  D  +I+    + FY  +  L + ++  +  L+ +  G +
Sbjct: 302 LAIGAF--GCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNR 359

Query: 435 RNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVI 494
            N+LRNRI+ + +   QL L T+ F  ++FL PT + +Y+ +  L+ LT       E   
Sbjct: 360 YNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFR 419

Query: 495 ALINHFPLFALLLRLKDPKR 514
             + +FP+   L  +K    
Sbjct: 420 RKLLNFPIEMFLKYIKKGFY 439
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
 gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 760

 Score =  375 bits (954), Expect = e-102
 Identities = 90/296 (30%), Positives = 150/296 (50%), Gaps = 32/296 (10%)

Query: 233 FPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFG 292
           +P+ +   +K    + +   +E          +  P++     +   NT+  ++ D++ G
Sbjct: 235 WPLSFLG-SKLSTCEQLRHRLEHLTLIFSTRKAENPAQL----MRKANTVASVLLDVALG 289

Query: 293 LILGAILIENR------DFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFL 346
           L+L + L          D LV  +  V      + L+ + + L   P G+K+N  L   L
Sbjct: 290 LMLLSWLHGRSRIGHLADALVPVADHVA-----EELQHLLQWLMGAPAGLKMNRALDQVL 344

Query: 347 SELFLWVIEFS------YTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAI 400
              FL+ I          + F++ ++    LS+ L LT+          A+SL  D  A+
Sbjct: 345 GRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV----------ALSLLSDIIAL 394

Query: 401 LSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFI 460
           L+F IY FY   ++LY  +++ ++SL+ LF GKK NVLR R+D   + LDQL +GTLLF 
Sbjct: 395 LTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFT 454

Query: 461 ILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLP 516
           IL+FL PT   +Y+ +T+LR+L + ++     ++ LIN  PL++L LRL  P RL 
Sbjct: 455 ILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRLCRPYRLA 510
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
          Length = 481

 Score =  362 bits (922), Expect = 7e-99
 Identities = 82/386 (21%), Positives = 154/386 (39%), Gaps = 37/386 (9%)

Query: 141 LFPAYCKALNELYPFIQTSQENLRGTMLNSVAAWCSSTCIYKMVAKIGFYLTFVICSIAS 200
            F       N    ++  + E L+   +                +           +  S
Sbjct: 82  CFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNLFVSGES----------ENRRS 131

Query: 201 L----VSS--LLNYSHFQLVNYSAFVQQIDLRCQQICYFPVQY-ERINKKDNIQNVGSMV 253
           L      S         +L   +A  Q             +QY   +     I+    M 
Sbjct: 132 LEYNDNDSTDCDFLELSRLNQPTADNQNKANNNANKS---IQYGLTLIADSPIKIFEYMA 188

Query: 254 EKDNSNSQFSHSYMPSKFYPDYIL---LYNTIWLIINDI----SFGLILGAILIENRDFL 306
           E    NS   H+ +  K + ++       +    I+ D        LIL ++  +  DFL
Sbjct: 189 ENVFINSIMVHTTI-YKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLATQPGDFL 247

Query: 307 VSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSYTTFIKRLI 366
           +  SH     ++ D L  + + L  +P+G+KLN  L NF  + F + IE  ++TF+   I
Sbjct: 248 IQISH-----YVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIEL-WSTFLD-FI 300

Query: 367 DPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQLNIMASL 426
           +P  L   + L I M+  +GF+F ++L +D  +++    + FY  +  LY+ +   ++ L
Sbjct: 301 EP--LVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVL 358

Query: 427 FNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTITI 486
           + +  G + N+L+ R + + +   QL L T+ F  ++FL PT + +Y+ +  L+ LT   
Sbjct: 359 WQVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFAT 418

Query: 487 EIFSEAVIALINHFPLFALLLRLKDP 512
                 V   + + P+   + RL   
Sbjct: 419 LSVFHFVRRKLMYLPIEVCIKRLLRG 444
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
          Length = 669

 Score =  352 bits (896), Expect = 8e-96
 Identities = 105/449 (23%), Positives = 186/449 (41%), Gaps = 54/449 (12%)

Query: 110 VGETLNYSKLEQHPRYKRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQ--------- 160
                +  +L+++ + K +  KL++    I+ F      L E +   Q            
Sbjct: 185 KENDCDKEQLDKYNKDKENKLKLNDKDEYIS-FNFIEDKLTESFHMNQIIHLINKKCVFT 243

Query: 161 ---ENLRGTMLNSVAAWCSST-------CIYKMVAKIGFYLTFVICSIASLVSSLLNY-- 208
              EN +                      I+  +  I  + T++I    SL++S L Y  
Sbjct: 244 KCLENYKNRYFVLKKEEILKKKKKQKKMSIFSYIVSIILFFTYII----SLINSCLYYII 299

Query: 209 SHFQLVNYSAFVQQIDLRCQQICY----FPVQYERINKKDNIQNVGSMVEKDNSNSQFSH 264
              +L +   F ++ D   Q   Y    FP ++  I +         M  K N       
Sbjct: 300 CTPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFR-------SFMKNKQN------- 345

Query: 265 SYMPSKFYPDYILLYNTIWLIINDISFGLILGAILIENRDFLVSASHRVLKFFLYDSLKT 324
              PS++Y    +L   I  +I DI  G ++  +L  N   L   S +   F+   +L +
Sbjct: 346 ---PSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIFYGTSTLTS 402

Query: 325 ITETLANNPLGIKLNAELANFLSELFLWVIEFSYTTFIKRLIDPKTLSSLLTLTIYMMFL 384
           I  TL  NPLG KLN    +F+  + + +++  +  F   +  P   S++L    Y   L
Sbjct: 403 ILGTLLQNPLGFKLNNNFTSFIGSILVSILD-KWDLFTNTI--PVNNSTVLNFVGYTS-L 458

Query: 385 VGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDH 444
           +GFSF +S  ID+   ++  + + Y    K+       M SL+ LF GKK N+L+ R+D 
Sbjct: 459 LGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDT 518

Query: 445 NYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFA 504
           NY+  +++LLGT+LF IL+FL PT+    + + ++ ++   I      +  +I + P + 
Sbjct: 519 NYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYI 578

Query: 505 LLLRLKDPKRLPGGISI---ELKTTVSNK 530
             ++    K +  G      E+      K
Sbjct: 579 FFIQPNCNKYISKGFKFTKYEVGEHELLK 607
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
 pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
 emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
          Length = 287

 Score =  269 bits (683), Expect = 7e-71
 Identities = 61/246 (24%), Positives = 100/246 (39%), Gaps = 15/246 (6%)

Query: 284 LIINDISFGLILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELA 343
           L++ D++F + L  I   N ++ V+         + D+L      L +NP G+KLN  + 
Sbjct: 29  LLLLDVAFSIYLWNIWTPNWEWTVNEFWD-QTGNVADNLNGTITWLRSNPAGLKLNTPVN 87

Query: 344 N-----FLSELFLWV-IEFSYTTFIKRL-IDPKTLSSLLTLTIYMMF-----LVGFSFAV 391
                 F   ++LW  + F +  F      D       L    +  F     + G S   
Sbjct: 88  ETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGFLRSDAFFRFIAYSLIGGISTFS 147

Query: 392 SLAIDFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQ 451
           ++  DF  I       F   ++KL +     +  L++L  GKK N LR R D       Q
Sbjct: 148 AMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLRERKDTVILDTRQ 207

Query: 452 LLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKD 511
             L T LF+IL+F+ PT+  +++ +  LR+    ++            F LFAL   L +
Sbjct: 208 QFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYFFATW--PFQLFALEKHLAE 265

Query: 512 PKRLPG 517
               P 
Sbjct: 266 KYGKPA 271
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana  (fragment)
 emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
          Length = 143

 Score =  155 bits (390), Expect = 2e-36
 Identities = 36/150 (24%), Positives = 66/150 (44%), Gaps = 11/150 (7%)

Query: 285 IINDISFGLILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELAN 344
           +  D+  G ++G  L+ N + + S      K F    L++ +  L   P G KLN ELA 
Sbjct: 1   MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 60

Query: 345 FLSELFLWVIEFSYTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFA----VSLAIDFFAI 400
            L  + L VI+   T +         ++S +   I ++ ++G +F      +  ID    
Sbjct: 61  VLGMVSLNVIQIWSTLW-------VFMASFIFCLIRVIAILGITFGATVSAAFVIDVITF 113

Query: 401 LSFPIYVFYRISSKLYHCQLNIMASLFNLF 430
            +  I   +   + +Y  Q+  +A+L+ LF
Sbjct: 114 ATLHIMALHWAITLVYSHQIQALAALWRLF 143
>emb|CAB68123.2| (AL137080) hypothetical protein [Arabidopsis thaliana]
          Length = 261

 Score =  116 bits (290), Expect = 8e-25
 Identities = 20/105 (19%), Positives = 35/105 (33%), Gaps = 13/105 (12%)

Query: 67  WNYKVADPCNVH---FRIPKLKFMQFYSSDPISLIIPEKEVGLHSSVGETLNYSKLEQHP 123
           WN  +   C+VH   +  P      F  S   S   P+ +  L             + H 
Sbjct: 167 WNGDIVSQCDVHVIVYDTPVFGSHHF--SLSFSNSSPQTKAPLKKPKWVD------DLHN 218

Query: 124 RYKRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQENLRGTML 168
           R   +  +       +N   A   A  ++   ++TS +N   + L
Sbjct: 219 RKPLNEMETVILS--LNCAAAAKIAYKKISTQLETSSQNFSISYL 261
>pir||T45795 hypothetical protein F28O9.20 - Arabidopsis thaliana
          Length = 242

 Score =  110 bits (273), Expect = 8e-23
 Identities = 16/86 (18%), Positives = 25/86 (28%), Gaps = 13/86 (15%)

Query: 67  WNYKVADPCNVH---FRIPKLKFMQFYSSDPISLIIPEKEVGLHSSVGETLNYSKLEQHP 123
           WN  +   C+VH   +  P      F  S   S   P+ +  L             + H 
Sbjct: 167 WNGDIVSQCDVHVIVYDTPVFGSHHF--SLSFSNSSPQTKAPLKKPKWVD------DLHN 218

Query: 124 RYKRDNKKLSETLNIINLFPAYCKAL 149
           R   +  +       +N   A    L
Sbjct: 219 RKPLNEMETVILS--LNCAAAAKNCL 242
>dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:1333114,
           evidence:ISS~phosphatidylinositol glycan, class
           Q~putative [Mus musculus]
          Length = 378

 Score = 64.2 bits (155), Expect = 6e-09
 Identities = 13/85 (15%), Positives = 34/85 (39%), Gaps = 11/85 (12%)

Query: 233 FPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLYNTIWLIINDISFG 292
           +P+ + R +K    + +   ++  +       +  P +     +   N +  ++ D++ G
Sbjct: 235 WPLSFIR-SKLSTCEQLHHRLKHLSFIFSTEKAQNPMQL----MRKANMLVSVLLDVALG 289

Query: 293 LILGAILIENR------DFLVSASH 311
           L+L + L  N       + LV  + 
Sbjct: 290 LLLLSWLHSNNRIGQLANALVPVAD 314
>gb|AAH10094.1|AAH10094 (BC010094) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 299

 Score = 50.6 bits (120), Expect = 8e-05
 Identities = 4/39 (10%), Positives = 14/39 (35%), Gaps = 1/39 (2%)

Query: 233 FPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKF 271
           +P+ +   +K    + +   +E          +  P++ 
Sbjct: 235 WPLSFLG-SKLSTCEQLRHRLEHLTLIFSTRKAENPAQL 272
CPU time:   148.89 user secs.	    4.42 sys. secs	  153.31 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.317    0.169    0.484 

Gapped
Lambda     K      H
   0.270   0.0591    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 366484031
Number of Sequences: 887402
Number of extensions: 17892364
Number of successful extensions: 74201
Number of sequences better than 10.0: 227
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 207
Number of HSP's that attempted gapping in prelim test: 73877
Number of HSP's gapped (non-prelim): 382
length of query: 609
length of database: 277,845,442
effective HSP length: 57
effective length of query: 552
effective length of database: 227,263,528
effective search space: 125449467456
effective search space used: 125449467456
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 76 (33.7 bits)