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GPI Anchor Biosynthesis Report: NP_004195.1 (PIG-Q family, Homo sapiens)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (581 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 1

Distribution of 19 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)




 gb|AAK61235.1|AE006464_3  (AE006464) N-acetylglucosamyl transfera...   980  0.0

 ref|NP_004195.1|  (NM_004204) phosphatidylinositol glycan, class ...   978  0.0

 dbj|BAA84658.1|  (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|...   978  0.0

 emb|CAB56148.1|  (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t...   975  0.0

 ref|NP_035952.1|  (NM_011822) glycosylphosphatidylinositol 1 homo...   974  0.0

 gb|AAC79574.1|  (AF030178) N-acetylglucosaminyl transferase compo...   971  0.0

 ref|XP_027458.2|  (XM_027458) similar to phosphatidylinositol gly...   859  0.0

 gb|AAH03917.1|AAH03917  (BC003917) Unknown (protein for IMAGE:348...   539  e-152

 dbj|BAB30464.1|  (AK016852) data source:MGD, source key:MGI:13331...   532  e-150

 gb|AAH10094.1|AAH10094  (BC010094) Similar to phosphatidylinosito...   502  e-141

 sp|O14357|GPI1_SCHPO  N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B...   346  7e-94

 ref|NP_011732.1|  (NC_001139) Participates in synthesis of N-acet...   343  5e-93

 gb|AAC49650.1|  (U77355) Gpi1+ [Schizosaccharomyces pombe]             339  7e-92

 emb|CAC10056.1|  (AJ298995) GPI1 protein [Drosophila melanogaster]     303  7e-81

 emb|CAC10057.1|  (AJ298996) GPI1 protein [Drosophila virilis]          285  1e-75

 ref|NP_191276.1|  (NM_115577) putative protein [Arabidopsis thali...   283  6e-75

 ref|NP_502086.1|  (NM_069685) F01G4.5.p [Caenorhabditis elegans] ...   276  1e-72

 emb|CAC10160.1|  (AJ249657) N-acetylglucosamine transferase [Plas...   273  5e-72

 pir||T45794  hypothetical protein F28O9.10 - Arabidopsis thaliana...   144  4e-33


Alignments


>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
           sapiens]
          Length = 581

 Score =  980 bits (2506), Expect = 0.0
 Identities = 579/581 (99%), Positives = 579/581 (99%)

Query: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60
           MVLKAFFPTCCVS DSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV
Sbjct: 1   MVLKAFFPTCCVSTDSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60

Query: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120
           AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA
Sbjct: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120

Query: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180
           PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR
Sbjct: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180

Query: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240
           FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL
Sbjct: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240

Query: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300
           GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS
Sbjct: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300

Query: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420
           LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL
Sbjct: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRV 540
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEA RPLRLLMQINPLPYSRV
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEAGRPLRLLMQINPLPYSRV 540

Query: 541 VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581
           VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD
Sbjct: 541 VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581





>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
           N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
          Length = 581

 Score =  978 bits (2502), Expect = 0.0
 Identities = 581/581 (100%), Positives = 581/581 (100%)

Query: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60
           MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV
Sbjct: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60

Query: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120
           AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA
Sbjct: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120

Query: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180
           PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR
Sbjct: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180

Query: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240
           FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL
Sbjct: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240

Query: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300
           GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS
Sbjct: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300

Query: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420
           LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL
Sbjct: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRV 540
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRV
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRV 540

Query: 541 VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581
           VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD
Sbjct: 541 VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581





>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
 gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
           (human) [Mus musculus]
          Length = 581

 Score =  978 bits (2502), Expect = 0.0
 Identities = 486/581 (83%), Positives = 524/581 (89%)

Query: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60
           MVLK FFPTCC SADSGLLVGRWVP QSSAV+LAV+HFPFIPIQVK+LLAQV++ASQV V
Sbjct: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60

Query: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120
           AVLGTWCH +QEP+ESLG FLE LG +F H+PWL+LCRERG   WSC+AT+ Q       
Sbjct: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120

Query: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180
             E+QVMLIFYDQR++LLS LH P VLP  Q G TTASTGGLA +FDTVARSEVLFR+D+
Sbjct: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180

Query: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240
           FDE PVRLSHWQSEGVEASIL ELA+RASGP+CLLLASLLSL+SA SACR++KLWPLSF+
Sbjct: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240

Query: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300
            SKLSTCEQL HRL+HL+ IFST KA+NP QLMRKAN + SVLLDVALGL+LLSWLH  +
Sbjct: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300

Query: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIG LA+ALVPVAD VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420
           LMSPF+EHILWHVGLSACLGLTVALS+ SDIIALLTFHIYCFYVYGARLYCLKI+GLSSL
Sbjct: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTIL+FLLPTTALYYLVFTLLRLLV+ V
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRV 540
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVL  EA RPLRLLMQINPL Y+ V
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV 540

Query: 541 VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581
           +H YRLP CGCHPKHSWG LCRKL  GELIYPWRQR DKQD
Sbjct: 541 MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581





>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
           component)) [Homo sapiens]
          Length = 583

 Score =  975 bits (2494), Expect = 0.0
 Identities = 579/583 (99%), Positives = 579/583 (99%), Gaps = 2/583 (0%)

Query: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60
           MVLKAFFPTCCVS DSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV
Sbjct: 1   MVLKAFFPTCCVSTDSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60

Query: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120
           AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA
Sbjct: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120

Query: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180
           PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR
Sbjct: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180

Query: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSAC--RVFKLWPLS 238
           FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSAC  RVFKLWPLS
Sbjct: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRRRVFKLWPLS 240

Query: 239 FLGSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHG 298
           FLGSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHG
Sbjct: 241 FLGSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHG 300

Query: 299 RSRIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISY 358
           RSRIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISY
Sbjct: 301 RSRIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISY 360

Query: 359 IHLMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLS 418
           IHLMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLS
Sbjct: 361 IHLMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLS 420

Query: 419 SLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVV 478
           SLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVV
Sbjct: 421 SLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVV 480

Query: 479 AVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYS 538
           AVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEA RPLRLLMQINPLPYS
Sbjct: 481 AVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEAGRPLRLLMQINPLPYS 540

Query: 539 RVVHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581
           RVVHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD
Sbjct: 541 RVVHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 583





>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
           glycosylphosphatidylinositol homolog (S. cerevisiae)
           [Mus musculus]
 dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
          Length = 581

 Score =  974 bits (2492), Expect = 0.0
 Identities = 485/581 (83%), Positives = 523/581 (89%)

Query: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60
           MVLK FFPTCC SADSGLLVGRWVP QSSAV+LAV+HFPFIPIQVK+LLAQV++ASQV V
Sbjct: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60

Query: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120
           AVLGTWCH +QEP+ESLG FLE LG +F H+PWL+LCRERG   WSC+AT+ Q       
Sbjct: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120

Query: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180
             E+QVMLIFYDQR++LLS LH P VLP  Q G TTASTGGLA +FDTVARSEVLFR+D+
Sbjct: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180

Query: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240
           FDE PVRLSHWQSEGVEASIL ELA+RASGP+CLLLASLLSL+SA SACR++KLWPLSF+
Sbjct: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240

Query: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300
            SKLSTCEQL HRL+HL+ IFST KA+NP QLMRKAN + SVLLDVALGL+LLSWLH  +
Sbjct: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300

Query: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIG LA+ALVPVAD VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420
           LMSPF+EHILWHVGLSACLGLTVALS+ SDIIALLTFHIYCFYVYGARLYCLKI+GLSSL
Sbjct: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTIL+FLLPTTALY LVFTLLRLLV+ V
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRV 540
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVL  EA RPLRLLMQINPL Y+ V
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV 540

Query: 541 VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581
           +H YRLP CGCHPKHSWG LCRKL  GELIYPWRQR DKQD
Sbjct: 541 MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581





>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
           musculus]
          Length = 581

 Score =  971 bits (2485), Expect = 0.0
 Identities = 493/581 (84%), Positives = 534/581 (91%)

Query: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60
           MVLKAFFPTCC SADSGLLVGRWVP Q+SAV+LAV+HFPFIPIQVK+LLAQV++ASQV V
Sbjct: 1   MVLKAFFPTCCASADSGLLVGRWVPGQNSAVILAVVHFPFIPIQVKELLAQVQKASQVQV 60

Query: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120
            VLGTWCH +QEPEESLG+FLE LGA+F H PWL+LCRE+G  FWSC+AT+ Q  +    
Sbjct: 61  TVLGTWCHRQQEPEESLGKFLEGLGAIFSHNPWLQLCREKGTRFWSCKATYHQMSSTLDT 120

Query: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180
           P EDQVMLIFYDQR++LLS LH P VLPD Q G +TASTGGLA +FDTVARSEVLFR+D+
Sbjct: 121 PTEDQVMLIFYDQRKLLLSWLHPPPVLPDCQIGDSTASTGGLADIFDTVARSEVLFRNDQ 180

Query: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240
           FDEGPVRLSHWQSEGVEASIL ELA+RASGP+CLLLASLLSL+SA SACR++KL PL+F+
Sbjct: 181 FDEGPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLGPLAFI 240

Query: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300
            SKLSTCEQL+HRL+HL+ IFST KA++P QLMRKAN + SVLLDVALGL+LLSWLH  +
Sbjct: 241 RSKLSTCEQLQHRLKHLSFIFSTEKAQSPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300

Query: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIG LA+ALVPVAD VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420
           LMSPF+EHILWHVGLSACLGLTVALS+ SDIIALLTFHIYCFYVYGARLYCLKI+GLSSL
Sbjct: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTIL+FLLPTTALYYLVFTLLRLLV+ V
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRV 540
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVL  EA RPLRLLMQINPLPYS V
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLPYSHV 540

Query: 541 VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581
           VHTYRLPSCGCHPKHSWG LCRKLF GELIYPWRQR DKQD
Sbjct: 541 VHTYRLPSCGCHPKHSWGTLCRKLFFGELIYPWRQREDKQD 581





>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
 gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 760

 Score =  859 bits (2196), Expect = 0.0
 Identities = 509/510 (99%), Positives = 509/510 (99%)

Query: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60
           MVLKAFFPTCCVS DSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV
Sbjct: 1   MVLKAFFPTCCVSTDSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60

Query: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120
           AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA
Sbjct: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120

Query: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180
           PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR
Sbjct: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180

Query: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240
           FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL
Sbjct: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240

Query: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300
           GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS
Sbjct: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300

Query: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420
           LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL
Sbjct: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLA 510
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLA
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLA 510





>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
          Length = 339

 Score =  539 bits (1375), Expect = e-152
 Identities = 302/339 (89%), Positives = 316/339 (93%)

Query: 243 KLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRI 302
           KLSTCEQL HRL+HL+ IFST KA+NP QLMRKAN + SVLLDVALGL+LLSWLH  +RI
Sbjct: 1   KLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRI 60

Query: 303 GHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLM 362
           G LA+ALVPVAD VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLM
Sbjct: 61  GQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLM 120

Query: 363 SPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWR 422
           SPF+EHILWHVGLSACLGLTVALS+ SDIIALLTFHIYCFYVYGARLYCLKI+GLSSLWR
Sbjct: 121 SPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWR 180

Query: 423 LFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQG 482
           LFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTIL+FLLPTTALYYLVFTLLRLLV+ VQG
Sbjct: 181 LFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQG 240

Query: 483 LIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRVVH 542
           LIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVL  EA RPLRLLMQINPL Y+ V+H
Sbjct: 241 LIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMH 300

Query: 543 TYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581
            YRLP CGCHPKHSWG LCRKL  GELIYPWRQR DKQD
Sbjct: 301 IYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 339





>dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:1333114,
           evidence:ISS~phosphatidylinositol glycan, class
           Q~putative [Mus musculus]
          Length = 378

 Score =  532 bits (1356), Expect = e-150
 Identities = 240/314 (76%), Positives = 271/314 (85%)

Query: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60
           MVLK FFPTCC SADSGLLVGRWVP QSSAV+LAV+HFPFIPIQVK+LLAQV++ASQV V
Sbjct: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60

Query: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120
           AVLGTWCH +QEP+ESLG FLE LG +F H+PWL+LCRERG   WSC+AT+ Q       
Sbjct: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120

Query: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180
             E+QVMLIFYDQR++LLS LH P VLP  Q G TTASTGGLA +FDTVARSEVLFR+D+
Sbjct: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180

Query: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240
           FDE PVRLSHWQSEGVEASIL ELA+RASGP+CLLLASLLSL+SA SACR++KLWPLSF+
Sbjct: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240

Query: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300
            SKLSTCEQL HRL+HL+ IFST KA+NP QLMRKAN + SVLLDVALGL+LLSWLH  +
Sbjct: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300

Query: 301 RIGHLADALVPVAD 314
           RIG LA+ALVPVAD
Sbjct: 301 RIGQLANALVPVAD 314





>gb|AAH10094.1|AAH10094 (BC010094) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 299

 Score =  502 bits (1281), Expect = e-141
 Identities = 274/274 (100%), Positives = 274/274 (100%)

Query: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60
           MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV
Sbjct: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60

Query: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120
           AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA
Sbjct: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120

Query: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180
           PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR
Sbjct: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180

Query: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240
           FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL
Sbjct: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240

Query: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMR 274
           GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMR
Sbjct: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMR 274





>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
           - fission yeast (Schizosaccharomyces pombe)
 emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
           protein gpi1. [Schizosaccharomyces pombe]
          Length = 653

 Score =  346 bits (879), Expect = 7e-94
 Identities = 88/308 (28%), Positives = 163/308 (52%), Gaps = 10/308 (3%)

Query: 267 ENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVP--VADHVAEELQHLL 324
           E+  + +R  N +  V  D+  G+ + S++       HL   L+     ++  + ++ ++
Sbjct: 307 EDYKEYIRFYNNLWLVANDMIFGITMSSFILENL---HLVVKLIENITFEYAIKNVRSMV 363

Query: 325 QWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTVA 384
            WL+  PAGLK+N  + + + +  ++ I +W +++    P+   ++  V +S   G ++ 
Sbjct: 364 IWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLM 423

Query: 385 LSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDLD 444
           ++L+SD ++++T HI+  Y+  +RLY  ++  + SL +LFRGKK NVLR R+DS  YDLD
Sbjct: 424 IALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLD 483

Query: 445 QLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRLC 504
           QL +GT+LFT+L+F LPT  ++Y  F L R+ V+    +   ++  +N  PL+   LR+ 
Sbjct: 484 QLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIK 543

Query: 505 RPYRLAAGVKFRVLRHEASRP-----LRLLMQINPLPYSRVVHTYRLPSCGCHPKHSWGA 559
            PYR+ +G+ F ++  E  +      L L     P+    +   YR  +      +    
Sbjct: 544 DPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKT 603

Query: 560 LCRKLFLG 567
               L +G
Sbjct: 604 TLISLLVG 611





>ref|NP_011732.1| (NC_001139) Participates in synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi1p
           [Saccharomyces cerevisiae]
 sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
 gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
          Length = 609

 Score =  343 bits (872), Expect = 5e-93
 Identities = 101/323 (31%), Positives = 162/323 (49%), Gaps = 27/323 (8%)

Query: 269 PAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVA-----EELQHL 323
               +   NT+  ++ D++ GL+L + L          D LV  +  V      + L+ +
Sbjct: 272 YPDYILLYNTIWLIINDISFGLILGAILIENR------DFLVSASHRVLKFFLYDSLKTI 325

Query: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACL---- 379
            + L   P G+K+N  L   L   FL+ I    SY      F++ ++    LS+ L    
Sbjct: 326 TETLANNPLGIKLNAELANFLSELFLWVIEF--SYT----TFIKRLIDPKTLSSLLTLTI 379

Query: 380 ------GLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLR 433
                 G + A+SL  D  A+L+F IY FY   ++LY  +++ ++SL+ LF GKK NVLR
Sbjct: 380 YMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLR 439

Query: 434 QRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINS 493
            R+D   + LDQL +GTLLF IL+FL PT   +Y+ +T+LR+L + ++     ++ LIN 
Sbjct: 440 NRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINH 499

Query: 494 LPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRVVHTYRLPSCGCHP 553
            PL++L LRL  P RL  G+   +    +++   L +Q NP+ +  +   Y L       
Sbjct: 500 FPLFALLLRLKDPKRLPGGISIELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRT 559

Query: 554 KHSWGALCRKLFLGELIYPWRQR 576
            +   A  RK+  GE I   R +
Sbjct: 560 NYFSFATVRKIVRGESIMVNRNK 582





>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
          Length = 517

 Score =  339 bits (862), Expect = 7e-92
 Identities = 87/308 (28%), Positives = 163/308 (52%), Gaps = 10/308 (3%)

Query: 267 ENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVP--VADHVAEELQHLL 324
           E+  + +R  N +  V  D+  G+ + S++       HL   L+     ++  + ++ ++
Sbjct: 171 EDYKEYIRFYNNLWLVANDMIFGITMSSFILENL---HLVVKLIENITFEYAIKNVRSMV 227

Query: 325 QWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTVA 384
            WL+  PAGLK+N  + + + +  ++ I +W +++    P+   ++  V +S   G ++ 
Sbjct: 228 IWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLM 287

Query: 385 LSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDLD 444
           ++L+SD ++++T HI+  Y+  +R+Y  ++  + SL +LFRGKK NVLR R+DS  YDLD
Sbjct: 288 IALISDFLSVMTIHIHLLYLASSRMYNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLD 347

Query: 445 QLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRLC 504
           QL +GT+LFT+L+F LPT  ++Y  F L R+ V+    +   ++  +N  PL+   LR+ 
Sbjct: 348 QLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIK 407

Query: 505 RPYRLAAGVKFRVLRHEASRP-----LRLLMQINPLPYSRVVHTYRLPSCGCHPKHSWGA 559
            PYR+ +G+ F ++  E  +      L L     P+    +   YR  +      +    
Sbjct: 408 DPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKLARRLISHYLSKT 467

Query: 560 LCRKLFLG 567
               L +G
Sbjct: 468 TLISLLVG 475





>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
          Length = 481

 Score =  303 bits (768), Expect = 7e-81
 Identities = 84/231 (36%), Positives = 137/231 (58%), Gaps = 2/231 (0%)

Query: 275 KANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHLLQWLMGAPAGL 334
           K +  A+++LD  LG++L+  L   S      D L+ ++ +V +EL  LL+ L G+P GL
Sbjct: 215 KRSRPANIVLDRILGIILMLILF--SLATQPGDFLIQISHYVIDELYGLLKVLEGSPIGL 272

Query: 335 KMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTVALSLLSDIIAL 394
           K+N  L+      F YHI LW +++  + P V  +   +G+  CLG T  ++LL D+I++
Sbjct: 273 KLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTFQIALLVDLISV 332

Query: 395 LTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFT 454
           +  H +CFY+Y   LY ++  GLS LW++ RG ++N+L+ R +S +Y   QL++ T+ F+
Sbjct: 333 IGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMNRQLYLATIFFS 392

Query: 455 ILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRLCR 505
            +LFLLPTT +YY+VF  L+ L  A   + H +   +  LP+     RL R
Sbjct: 393 AILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRLLR 443





>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
          Length = 473

 Score =  285 bits (723), Expect = 1e-75
 Identities = 83/273 (30%), Positives = 152/273 (55%), Gaps = 6/273 (2%)

Query: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323
           R  +    +  K + ++++++D ALG++L+  L   + + H  D L+ ++  +  +L  L
Sbjct: 197 RHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLF--TLVSHPGDFLIQISHIIIHQLYSL 254

Query: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV 383
           L+ L G+P GLK+N  L+      F YHI LW +++ L+ P V  +   +G   CLG T 
Sbjct: 255 LKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTY 314

Query: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443
            ++LL+D+I+++  H +CFYVY   L  +++ GL+ LW++ RG ++N+LR R+++ +Y  
Sbjct: 315 QIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMN 374

Query: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503
            QL++ T+ F+ +LFL PTT +YY+VF  L+ L  A    +      + + P+      +
Sbjct: 375 RQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYI 434

Query: 504 CRP-YRLAAGVKFRVLRHEASRPLRLLMQINPL 535
            +  Y +      RVL     +PL  + + + L
Sbjct: 435 KKGFYEIDC---LRVLDIPLQKPLFFIHRNSKL 464





>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
 pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
 emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
          Length = 680

 Score =  283 bits (718), Expect = 6e-75
 Identities = 85/263 (32%), Positives = 137/263 (51%), Gaps = 12/263 (4%)

Query: 260 IFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEE 319
           I   + AE  A  +++ +T +++ +D+ LG ++   L   +           V D   E 
Sbjct: 313 ISCVKHAEEAA--LQRHSTWSAMAVDLVLGNLIGLGLLFNTE-----SVCSFVFDFAKEF 365

Query: 320 LQHLLQ----WLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG- 374
              +L+    WLMG PAG K+N  L  VLG   L  I +W +    M+ F+  ++  +  
Sbjct: 366 TNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAI 425

Query: 375 LSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQ 434
           L    G TV+ + + D+I   T HI   +     +Y  +I  L++LWRLFRG+K N LRQ
Sbjct: 426 LGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFRGRKLNPLRQ 485

Query: 435 RVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 494
           R+DS  Y + Q  +G+LLFT LL LLPTT+++Y+ FT+    + ++  LI   + +I++ 
Sbjct: 486 RMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTTINSICMLIEFAISVIHAT 545

Query: 495 PLYSLGLRLCRPYRLAAGVKFRV 517
           P   + + L R  R   GV F +
Sbjct: 546 PYAEVMIWLVRRKRFPCGVWFEM 568





>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
 pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
 emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
          Length = 287

 Score =  276 bits (699), Expect = 1e-72
 Identities = 87/266 (32%), Positives = 123/266 (45%), Gaps = 24/266 (9%)

Query: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323
           R  E    L+R  +    +LLDVA  + L  W           +       +VA+ L   
Sbjct: 12  RTLEAKILLVRTFSFTRLLLLDVAFSIYL--WNIWTPNWEWTVNEFWDQTGNVADNLNGT 69

Query: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLT- 382
           + WL   PAGLK+N  +++ L  FF YHI+LW S +     F  +  +H   SA +G   
Sbjct: 70  ITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLR 127

Query: 383 -------VALSLLS----------DIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFR 425
                  +A SL+           D   +   H  CF  Y  +L  L  + L+ LW L R
Sbjct: 128 SDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVR 187

Query: 426 GKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIH 485
           GKKWN LR+R D+   D  Q F+ T LF ILLF+LPT  +Y++VF  LRL V A+Q +++
Sbjct: 188 GKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLY 247

Query: 486 LLVDLINSLPLYSLGLRLCRPYRLAA 511
                     L++L   L   Y   A
Sbjct: 248 FFATW--PFQLFALEKHLAEKYGKPA 271





>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
          Length = 669

 Score =  273 bits (693), Expect = 5e-72
 Identities = 67/262 (25%), Positives = 123/262 (46%), Gaps = 22/262 (8%)

Query: 270 AQ------LMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323
                   L+        + L +   ++ L ++  +++I +    L  +           
Sbjct: 355 ILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIFYGTSTLTSI----------- 403

Query: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV 383
           L  L+  P G K+N      +G   +  +  W  + + +      +L  VG ++ LG + 
Sbjct: 404 LGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSLLGFSF 463

Query: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443
            LS + D +  +T H+   Y++  ++  L    + SL+ LF GKKWN+L+ RVD+  Y  
Sbjct: 464 FLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSN 523

Query: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503
           +++ +GT+LFTIL+FL PT  +  LVF L+ L++  +  L+ ++  +I   P Y   ++ 
Sbjct: 524 EEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQP 583

Query: 504 -CRPYRLAAGVKF---RVLRHE 521
            C  Y ++ G KF    V  HE
Sbjct: 584 NCNKY-ISKGFKFTKYEVGEHE 604





>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana  (fragment)
 emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
          Length = 143

 Score =  144 bits (361), Expect = 4e-33
 Identities = 43/148 (29%), Positives = 68/148 (45%), Gaps = 10/148 (6%)

Query: 282 VLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHLLQ----WLMGAPAGLKMN 337
           + +D+ LG ++   L   +           V D   E    +L+    WLMG PAG K+N
Sbjct: 1   MAVDLVLGNLIGLGLLFNTE-----SVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLN 55

Query: 338 RALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG-LSACLGLTVALSLLSDIIALLT 396
             L  VLG   L  I +W +    M+ F+  ++  +  L    G TV+ + + D+I   T
Sbjct: 56  TELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFAT 115

Query: 397 FHIYCFYVYGARLYCLKIHGLSSLWRLF 424
            HI   +     +Y  +I  L++LWRLF
Sbjct: 116 LHIMALHWAITLVYSHQIQALAALWRLF 143





CPU time:   152.00 user secs.	    2.35 sys. secs	  154.35 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.315    0.165    0.518 

Gapped
Lambda     K      H
   0.270   0.0578    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 387424936
Number of Sequences: 887402
Number of extensions: 19123067
Number of successful extensions: 71878
Number of sequences better than 10.0: 103
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 82
Number of HSP's that attempted gapping in prelim test: 71757
Number of HSP's gapped (non-prelim): 163
length of query: 581
length of database: 277,845,442
effective HSP length: 52
effective length of query: 529
effective length of database: 231,700,538
effective search space: 122569584602
effective search space used: 122569584602
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.8 bits)
S2: 76 (33.7 bits)