Sequences with E-value BETTER than threshold (0.002)
emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 680 0.0
emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 641 0.0
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 350 4e-95
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 333 3e-90
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 333 4e-90
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 333 5e-90
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 322 6e-87
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 322 1e-86
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 318 1e-85
ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 307 3e-82
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 306 6e-82
ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 271 2e-71
ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 254 3e-66
sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 251 2e-65
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 245 2e-63
emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 233 4e-60
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 156 6e-37
Alignments
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
Length = 473
Score = 680 bits (1737), Expect = 0.0
Identities = 473/473 (100%), Positives = 473/473 (100%)
Query: 1 MSIKIFLPTEYFYLKRACNLYGQLHISEDNVVVYYVVDADNNKLDSDTNKMRFLGSILCS 60
MSIKIFLPTEYFYLKRACNLYGQLHISEDNVVVYYVVDADNNKLDSDTNKMRFLGSILCS
Sbjct: 1 MSIKIFLPTEYFYLKRACNLYGQLHISEDNVVVYYVVDADNNKLDSDTNKMRFLGSILCS 60
Query: 61 DSYADSNYCRSHDVVLCFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSLVIKDN 120
DSYADSNYCRSHDVVLCFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSLVIKDN
Sbjct: 61 DSYADSNYCRSHDVVLCFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSLVIKDN 120
Query: 121 NTLAHWTRENQINDECDFYMLAQLVQPNPDTCRKSNDLWFYGSQSLCLLANIPMQMFQYI 180
NTLAHWTRENQINDECDFYMLAQLVQPNPDTCRKSNDLWFYGSQSLCLLANIPMQMFQYI
Sbjct: 121 NTLAHWTRENQINDECDFYMLAQLVQPNPDTCRKSNDLWFYGSQSLCLLANIPMQMFQYI 180
Query: 181 VGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFL 240
VGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFL
Sbjct: 181 VGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFL 240
Query: 241 IQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQV 300
IQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQV
Sbjct: 241 IQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQV 300
Query: 301 FLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRY 360
FLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRY
Sbjct: 301 FLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRY 360
Query: 361 NILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRR 420
NILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRR
Sbjct: 361 NILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRR 420
Query: 421 KLLNFPIEMFLKYIKKGFYEIDCLRVLDIPLQKPLFFIHRNSKLIIFVYKLRV 473
KLLNFPIEMFLKYIKKGFYEIDCLRVLDIPLQKPLFFIHRNSKLIIFVYKLRV
Sbjct: 421 KLLNFPIEMFLKYIKKGFYEIDCLRVLDIPLQKPLFFIHRNSKLIIFVYKLRV 473
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
Length = 481
Score = 641 bits (1637), Expect = 0.0
Identities = 281/479 (58%), Positives = 360/479 (74%), Gaps = 9/479 (1%)
Query: 1 MSIKIFLPTEYFYLKRACNLYGQLHISEDNVVVYYVVDA-----DNNKLDSDTNKMRFLG 55
MSIKI+LP Y+YL ++ NLYGQ+ I+EDNVV YYV++A + ++ + +RFLG
Sbjct: 1 MSIKIYLPVNYYYLNKSTNLYGQVQINEDNVVSYYVLEASDLEFSDKAINHEKGNLRFLG 60
Query: 56 SILCSDSYADSNYCRSHDVVLCFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSL 115
SIL D AD N ++ LCFTY++ N++L++ GITPE L +K+ILYDKQ VR+L
Sbjct: 61 SILNEDFNADQNQFLKFNMRLCFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNL 120
Query: 116 VI--KDNNTLAHWTRENQINDECDFYMLAQLVQPNPDTCRKSNDLWFYGSQ-SLCLLANI 172
+ + N + +N D CDF L++L QP D K+N+ Q L L+A+
Sbjct: 121 FVSGESENRRSLEYNDNDSTD-CDFLELSRLNQPTADNQNKANNNANKSIQYGLTLIADS 179
Query: 173 PMQMFQYIVGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTL 232
P+++F+Y+ N FINSI+ HT IY+H+KEWQ+ K SR +NI++DR LGIILML+LF+L
Sbjct: 180 PIKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL 239
Query: 233 VSHPGDFLIQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDL 292
+ PGDFLIQISH +I +LY LLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLD
Sbjct: 240 ATQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDF 299
Query: 293 IEPIVRQVFLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLW 352
IEP+VRQVFLAIG GCLGFT+QIALL DLIS++GLH+HCFY+YTKVL NVE +GL+VLW
Sbjct: 300 IEPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLW 359
Query: 353 QVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATL 412
QVVRGNRYNIL+ R E+HNYMNRQLYLATIFFSAILFL PTTLVYY+VFA LKALT ATL
Sbjct: 360 QVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATL 419
Query: 413 ATLECFRRKLLNFPIEMFLKYIKKGFYEIDCLRVLDIPLQKPLFFIHRNSKLIIFVYKL 471
+ RRKL+ PIE+ +K + +G +EIDC+++ D+ + F +H+ K+ + VYK+
Sbjct: 420 SVFHFVRRKLMYLPIEVCIKRLLRGCHEIDCIQIKDVSHHERAFLMHKQQKINVTVYKI 478
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
sapiens]
gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
sapiens]
Length = 760
Score = 350 bits (889), Expect = 4e-95
Identities = 77/251 (30%), Positives = 142/251 (55%), Gaps = 2/251 (0%)
Query: 188 SIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLF--TLVSHPGDFLIQISH 245
+T R + + K + ++++++D ALG++L+ L + + H D L+ ++
Sbjct: 255 EHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVAD 314
Query: 246 IIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIG 305
+ +L LL+ L G+P GLK+N L+ F YHI LW +++ L+ P V + +G
Sbjct: 315 HVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG 374
Query: 306 AFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRN 365
CLG T ++LL+D+I+++ H +CFYVY L +++ GL+ LW++ RG ++N+LR
Sbjct: 375 LSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQ 434
Query: 366 RIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNF 425
R+++ +Y QL++ T+ F+ +LFL PTT +YY+VF L+ L A + + +
Sbjct: 435 RVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 494
Query: 426 PIEMFLKYIKK 436
P+ + +
Sbjct: 495 PLYSLGLRLCR 505
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
component)) [Homo sapiens]
Length = 583
Score = 333 bits (847), Expect = 3e-90
Identities = 77/251 (30%), Positives = 142/251 (55%), Gaps = 2/251 (0%)
Query: 188 SIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLF--TLVSHPGDFLIQISH 245
+T R + + K + ++++++D ALG++L+ L + + H D L+ ++
Sbjct: 257 EHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVAD 316
Query: 246 IIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIG 305
+ +L LL+ L G+P GLK+N L+ F YHI LW +++ L+ P V + +G
Sbjct: 317 HVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG 376
Query: 306 AFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRN 365
CLG T ++LL+D+I+++ H +CFYVY L +++ GL+ LW++ RG ++N+LR
Sbjct: 377 LSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQ 436
Query: 366 RIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNF 425
R+++ +Y QL++ T+ F+ +LFL PTT +YY+VF L+ L A + + +
Sbjct: 437 RVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 496
Query: 426 PIEMFLKYIKK 436
P+ + +
Sbjct: 497 PLYSLGLRLCR 507
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
sapiens]
Length = 581
Score = 333 bits (846), Expect = 4e-90
Identities = 77/251 (30%), Positives = 142/251 (55%), Gaps = 2/251 (0%)
Query: 188 SIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLF--TLVSHPGDFLIQISH 245
+T R + + K + ++++++D ALG++L+ L + + H D L+ ++
Sbjct: 255 EHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVAD 314
Query: 246 IIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIG 305
+ +L LL+ L G+P GLK+N L+ F YHI LW +++ L+ P V + +G
Sbjct: 315 HVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG 374
Query: 306 AFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRN 365
CLG T ++LL+D+I+++ H +CFYVY L +++ GL+ LW++ RG ++N+LR
Sbjct: 375 LSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQ 434
Query: 366 RIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNF 425
R+++ +Y QL++ T+ F+ +LFL PTT +YY+VF L+ L A + + +
Sbjct: 435 RVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 494
Query: 426 PIEMFLKYIKK 436
P+ + +
Sbjct: 495 PLYSLGLRLCR 505
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
N-acetylglucosaminyl transferase component Gpi1 [Homo
sapiens]
gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
sapiens]
dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
Length = 581
Score = 333 bits (846), Expect = 5e-90
Identities = 77/251 (30%), Positives = 142/251 (55%), Gaps = 2/251 (0%)
Query: 188 SIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLF--TLVSHPGDFLIQISH 245
+T R + + K + ++++++D ALG++L+ L + + H D L+ ++
Sbjct: 255 EHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVAD 314
Query: 246 IIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIG 305
+ +L LL+ L G+P GLK+N L+ F YHI LW +++ L+ P V + +G
Sbjct: 315 HVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG 374
Query: 306 AFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRN 365
CLG T ++LL+D+I+++ H +CFYVY L +++ GL+ LW++ RG ++N+LR
Sbjct: 375 LSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQ 434
Query: 366 RIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNF 425
R+++ +Y QL++ T+ F+ +LFL PTT +YY+VF L+ L A + + +
Sbjct: 435 RVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 494
Query: 426 PIEMFLKYIKK 436
P+ + +
Sbjct: 495 PLYSLGLRLCR 505
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
musculus]
Length = 581
Score = 322 bits (819), Expect = 6e-87
Identities = 69/234 (29%), Positives = 134/234 (56%), Gaps = 2/234 (0%)
Query: 205 IYTKGSRLSNIMIDRALGIILMLVLFTL--VSHPGDFLIQISHIIIHQLYSLLKVLEGSP 262
+ K + L ++++D ALG++L+ L + + + L+ ++ + +L LL+ L G+P
Sbjct: 272 LMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAP 331
Query: 263 IGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADL 322
GLK+N L+ F YHI LW +++ L+ P + + +G CLG T +++ +D+
Sbjct: 332 AGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDI 391
Query: 323 ISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATI 382
I+++ H +CFYVY L +++ GL+ LW++ RG ++N+LR R+++ +Y QL++ T+
Sbjct: 392 IALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTL 451
Query: 383 FFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIKK 436
F+ ++FL PTT +YY+VF L+ L + + + P+ + +
Sbjct: 452 LFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCR 505
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
(human) [Mus musculus]
Length = 581
Score = 322 bits (817), Expect = 1e-86
Identities = 69/234 (29%), Positives = 134/234 (56%), Gaps = 2/234 (0%)
Query: 205 IYTKGSRLSNIMIDRALGIILMLVLFTL--VSHPGDFLIQISHIIIHQLYSLLKVLEGSP 262
+ K + L ++++D ALG++L+ L + + + L+ ++ + +L LL+ L G+P
Sbjct: 272 LMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAP 331
Query: 263 IGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADL 322
GLK+N L+ F YHI LW +++ L+ P + + +G CLG T +++ +D+
Sbjct: 332 AGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDI 391
Query: 323 ISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATI 382
I+++ H +CFYVY L +++ GL+ LW++ RG ++N+LR R+++ +Y QL++ T+
Sbjct: 392 IALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTL 451
Query: 383 FFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIKK 436
F+ ++FL PTT +YY+VF L+ L + + + P+ + +
Sbjct: 452 LFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCR 505
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
glycosylphosphatidylinositol homolog (S. cerevisiae)
[Mus musculus]
dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
Length = 581
Score = 318 bits (808), Expect = 1e-85
Identities = 68/234 (29%), Positives = 133/234 (56%), Gaps = 2/234 (0%)
Query: 205 IYTKGSRLSNIMIDRALGIILMLVLFTL--VSHPGDFLIQISHIIIHQLYSLLKVLEGSP 262
+ K + L ++++D ALG++L+ L + + + L+ ++ + +L LL+ L G+P
Sbjct: 272 LMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAP 331
Query: 263 IGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADL 322
GLK+N L+ F YHI LW +++ L+ P + + +G CLG T +++ +D+
Sbjct: 332 AGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDI 391
Query: 323 ISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATI 382
I+++ H +CFYVY L +++ GL+ LW++ RG ++N+LR R+++ +Y QL++ T+
Sbjct: 392 IALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTL 451
Query: 383 FFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIKK 436
F+ ++FL PTT +Y +VF L+ L + + + P+ + +
Sbjct: 452 LFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCR 505
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
Length = 680
Score = 307 bits (780), Expect = 3e-82
Identities = 59/269 (21%), Positives = 112/269 (40%), Gaps = 5/269 (1%)
Query: 171 NIPMQMFQYIVGNKFINSI-ITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVL 229
N ++ Q + F+ I + +H +E + + S S + +D LG ++ L L
Sbjct: 290 NFRIRSCQILYWPIFLEEIDMMSISCVKHAEE--AALQRHSTWSAMAVDLVLGNLIGLGL 347
Query: 230 FTLVSHPGDFLIQISHIIIHQ-LYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWST 288
F+ + + L S L G P G KLN L I++WST
Sbjct: 348 LFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWST 407
Query: 289 FLDLIEPIVRQVFLAIGAFGC-LGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKG 347
+ + + I G G T A + D+I+ LH + ++ + +++
Sbjct: 408 LWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQA 467
Query: 348 LTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKAL 407
L LW++ RG + N LR R++++ Y +Q + ++ F+ +L L PTT V+Y+ F
Sbjct: 468 LAALWRLFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTT 527
Query: 408 TCATLATLECFRRKLLNFPIEMFLKYIKK 436
+ +E + P + ++ +
Sbjct: 528 INSICMLIEFAISVIHATPYAEVMIWLVR 556
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
Length = 339
Score = 306 bits (776), Expect = 6e-82
Identities = 69/234 (29%), Positives = 134/234 (56%), Gaps = 2/234 (0%)
Query: 205 IYTKGSRLSNIMIDRALGIILMLVLFTL--VSHPGDFLIQISHIIIHQLYSLLKVLEGSP 262
+ K + L ++++D ALG++L+ L + + + L+ ++ + +L LL+ L G+P
Sbjct: 30 LMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAP 89
Query: 263 IGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADL 322
GLK+N L+ F YHI LW +++ L+ P + + +G CLG T +++ +D+
Sbjct: 90 AGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDI 149
Query: 323 ISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATI 382
I+++ H +CFYVY L +++ GL+ LW++ RG ++N+LR R+++ +Y QL++ T+
Sbjct: 150 IALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTL 209
Query: 383 FFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIKK 436
F+ ++FL PTT +YY+VF L+ L + + + P+ + +
Sbjct: 210 LFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCR 263
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
N-acetylglucoaminylphosphatidylinositol, the first
intermediate in synthesis of
glycosylphosphatidylinositol (GPI) anchors; Gpi1p
[Saccharomyces cerevisiae]
sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
cerevisiae)
emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
Length = 609
Score = 271 bits (687), Expect = 2e-71
Identities = 71/272 (26%), Positives = 135/272 (49%), Gaps = 17/272 (6%)
Query: 174 MQMFQYIVGNKFINSIITHTVI-YRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTL 232
+Q +V NS +H+ + + Y ++ +Y + + I+ D + G+IL +L
Sbjct: 246 IQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLY---NTIWLIINDISFGLILGAILI-- 300
Query: 233 VSHPGDFLIQISHIII-----HQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIEL-W 286
DFL+ SH ++ L ++ + L +P+G+KLN L NF + F + IE +
Sbjct: 301 --ENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSY 358
Query: 287 STFLD-LIEPIVRQVFLAIGAFG--CLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNV 343
+TF+ LI+P L + + +GF++ ++L D +I+ + FY + L +
Sbjct: 359 TTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHC 418
Query: 344 EVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFAT 403
++ + L+ + G + N+LRNRI+ + + QL L T+ F ++FL PT + +Y+ +
Sbjct: 419 QLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTV 478
Query: 404 LKALTCATLATLECFRRKLLNFPIEMFLKYIK 435
L+ LT E + +FP+ L +K
Sbjct: 479 LRMLTITIEIFSEAVIALINHFPLFALLLRLK 510
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
Length = 287
Score = 254 bits (643), Expect = 3e-66
Identities = 71/260 (27%), Positives = 109/260 (41%), Gaps = 20/260 (7%)
Query: 189 IITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFLIQISHIII 248
I + V+ R + + S +++D A I L + + + +
Sbjct: 4 ITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVA 63
Query: 249 HQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFL---------------DLI 293
L + L +P GLKLN +N F YHI LW++ L I
Sbjct: 64 DNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFI 123
Query: 294 EPIVRQVFLAIGAFGCL-GFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLW 352
+ F A+ + G + A++ D I LH +CF Y L + LTVLW
Sbjct: 124 GFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLW 183
Query: 353 QVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATL 412
+VRG ++N LR R + RQ +LAT F +LF+ PT VY+VVF L+ L + L
Sbjct: 184 SLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLR-LAVSAL 242
Query: 413 ATLECFRRKLLNFPIEMFLK 432
T+ F +P ++F
Sbjct: 243 QTVLYF---FATWPFQLFAL 259
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
- fission yeast (Schizosaccharomyces pombe)
emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
protein gpi1. [Schizosaccharomyces pombe]
Length = 653
Score = 251 bits (635), Expect = 2e-65
Identities = 73/259 (28%), Positives = 121/259 (46%), Gaps = 7/259 (2%)
Query: 178 QYIVGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPG 237
QY+ F S YKE+ Y + L + D GI + + +
Sbjct: 291 QYMKLWVFRKSKRVAI---EDYKEYIRFY---NNLWLVANDMIFGITMSSFILENLHLVV 344
Query: 238 DFLIQIS-HIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPI 296
+ I+ I + S++ L +P GLKLN + F + + I++WS FL P
Sbjct: 345 KLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPW 404
Query: 297 VRQVFLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVR 356
+ + G G + IAL++D +S++ +H H Y+ + L N +++ + L Q+ R
Sbjct: 405 TPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFR 464
Query: 357 GNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLE 416
G + N+LRNRI+++ Y QL L TI F+ ++F PT V+Y FA + LA E
Sbjct: 465 GKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICE 524
Query: 417 CFRRKLLNFPIEMFLKYIK 435
L +FP+ + + IK
Sbjct: 525 TMLAFLNHFPLFVTMLRIK 543
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
Length = 517
Score = 245 bits (620), Expect = 2e-63
Identities = 72/259 (27%), Positives = 121/259 (45%), Gaps = 7/259 (2%)
Query: 178 QYIVGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPG 237
QY+ F S YKE+ Y + L + D GI + + +
Sbjct: 155 QYMKLWVFRKSKRVAI---EDYKEYIRFY---NNLWLVANDMIFGITMSSFILENLHLVV 208
Query: 238 DFLIQIS-HIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPI 296
+ I+ I + S++ L +P GLKLN + F + + I++WS FL P
Sbjct: 209 KLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPW 268
Query: 297 VRQVFLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVR 356
+ + G G + IAL++D +S++ +H H Y+ + + N +++ + L Q+ R
Sbjct: 269 TPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRMYNWQLRVIYSLLQLFR 328
Query: 357 GNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLE 416
G + N+LRNRI+++ Y QL L TI F+ ++F PT V+Y FA + LA E
Sbjct: 329 GKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICE 388
Query: 417 CFRRKLLNFPIEMFLKYIK 435
L +FP+ + + IK
Sbjct: 389 TMLAFLNHFPLFVTMLRIK 407
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
Length = 669
Score = 233 bits (590), Expect = 4e-60
Identities = 63/236 (26%), Positives = 116/236 (48%), Gaps = 9/236 (3%)
Query: 211 RLSNIMIDRALGIILMLVLFTLVSHPGDFLIQISHII--IHQLYSLLKVLEGSPIGLKLN 268
R+ N++ID LG ++ L+L+ V + ++ + + I L S+L L +P+G KLN
Sbjct: 359 RIINLIIDIFLGFLIFLLLYFNVINL-HYISEKAQIFYGTSTLTSILGTLLQNPLGFKLN 417
Query: 269 IHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADLISIVGL 328
+ +F ++ W F + I V +G LGF++ ++ + D + V
Sbjct: 418 NNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTA 477
Query: 329 HAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAIL 388
H Y++ K + + K + L+ + G ++NIL+ R++ + Y N ++ L TI F+ ++
Sbjct: 478 HVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILI 537
Query: 389 FLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFL------KYIKKGF 438
FL+PT V +VF + + + L + +L P +F KYI KGF
Sbjct: 538 FLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGF 593
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana (fragment)
emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
Length = 143
Score = 156 bits (393), Expect = 6e-37
Identities = 32/143 (22%), Positives = 56/143 (38%), Gaps = 2/143 (1%)
Query: 215 IMIDRALGIILMLVLFTLVSHPGDFLIQISHIIIHQ-LYSLLKVLEGSPIGLKLNIHLNN 273
+ +D LG ++ L L F+ + + L S L G P G KLN L
Sbjct: 1 MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 60
Query: 274 FFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGC-LGFTYQIALLADLISIVGLHAHC 332
I++WST + + + I G G T A + D+I+ LH
Sbjct: 61 VLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMA 120
Query: 333 FYVYTKVLNNVEVKGLTVLWQVV 355
+ ++ + +++ L LW++
Sbjct: 121 LHWAITLVYSHQIQALAALWRLF 143
CPU time: 141.23 user secs. 2.65 sys. secs 143.88 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.315 0.170 0.506
Gapped
Lambda K H
0.270 0.0597 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 328297406
Number of Sequences: 887402
Number of extensions: 17423262
Number of successful extensions: 78471
Number of sequences better than 10.0: 211
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 193
Number of HSP's that attempted gapping in prelim test: 78161
Number of HSP's gapped (non-prelim): 395
length of query: 473
length of database: 277,845,442
effective HSP length: 52
effective length of query: 421
effective length of database: 231,700,538
effective search space: 97545926498
effective search space used: 97545926498
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.7 bits)
S2: 75 (33.3 bits)