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GPI Anchor Biosynthesis Report: CAC10057.1 (PIG-Q family, Drosophila virilis)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (473 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 17 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 680 0.0
emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 641 0.0
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 350 4e-95
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 333 3e-90
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 333 4e-90
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 333 5e-90
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 322 6e-87
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 322 1e-86
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 318 1e-85
ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 307 3e-82
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 306 6e-82
ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 271 2e-71
ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 254 3e-66
sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 251 2e-65
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 245 2e-63
emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 233 4e-60
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 156 6e-37
Alignments
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
          Length = 473

 Score =  680 bits (1737), Expect = 0.0
 Identities = 473/473 (100%), Positives = 473/473 (100%)

Query: 1   MSIKIFLPTEYFYLKRACNLYGQLHISEDNVVVYYVVDADNNKLDSDTNKMRFLGSILCS 60
           MSIKIFLPTEYFYLKRACNLYGQLHISEDNVVVYYVVDADNNKLDSDTNKMRFLGSILCS
Sbjct: 1   MSIKIFLPTEYFYLKRACNLYGQLHISEDNVVVYYVVDADNNKLDSDTNKMRFLGSILCS 60

Query: 61  DSYADSNYCRSHDVVLCFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSLVIKDN 120
           DSYADSNYCRSHDVVLCFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSLVIKDN
Sbjct: 61  DSYADSNYCRSHDVVLCFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSLVIKDN 120

Query: 121 NTLAHWTRENQINDECDFYMLAQLVQPNPDTCRKSNDLWFYGSQSLCLLANIPMQMFQYI 180
           NTLAHWTRENQINDECDFYMLAQLVQPNPDTCRKSNDLWFYGSQSLCLLANIPMQMFQYI
Sbjct: 121 NTLAHWTRENQINDECDFYMLAQLVQPNPDTCRKSNDLWFYGSQSLCLLANIPMQMFQYI 180

Query: 181 VGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFL 240
           VGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFL
Sbjct: 181 VGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFL 240

Query: 241 IQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQV 300
           IQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQV
Sbjct: 241 IQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQV 300

Query: 301 FLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRY 360
           FLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRY
Sbjct: 301 FLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRY 360

Query: 361 NILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRR 420
           NILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRR
Sbjct: 361 NILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRR 420

Query: 421 KLLNFPIEMFLKYIKKGFYEIDCLRVLDIPLQKPLFFIHRNSKLIIFVYKLRV 473
           KLLNFPIEMFLKYIKKGFYEIDCLRVLDIPLQKPLFFIHRNSKLIIFVYKLRV
Sbjct: 421 KLLNFPIEMFLKYIKKGFYEIDCLRVLDIPLQKPLFFIHRNSKLIIFVYKLRV 473
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
          Length = 481

 Score =  641 bits (1637), Expect = 0.0
 Identities = 281/479 (58%), Positives = 360/479 (74%), Gaps = 9/479 (1%)

Query: 1   MSIKIFLPTEYFYLKRACNLYGQLHISEDNVVVYYVVDA-----DNNKLDSDTNKMRFLG 55
           MSIKI+LP  Y+YL ++ NLYGQ+ I+EDNVV YYV++A      +  ++ +   +RFLG
Sbjct: 1   MSIKIYLPVNYYYLNKSTNLYGQVQINEDNVVSYYVLEASDLEFSDKAINHEKGNLRFLG 60

Query: 56  SILCSDSYADSNYCRSHDVVLCFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSL 115
           SIL  D  AD N     ++ LCFTY++   N++L++ GITPE L  +K+ILYDKQ VR+L
Sbjct: 61  SILNEDFNADQNQFLKFNMRLCFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNL 120

Query: 116 VI--KDNNTLAHWTRENQINDECDFYMLAQLVQPNPDTCRKSNDLWFYGSQ-SLCLLANI 172
            +  +  N  +    +N   D CDF  L++L QP  D   K+N+      Q  L L+A+ 
Sbjct: 121 FVSGESENRRSLEYNDNDSTD-CDFLELSRLNQPTADNQNKANNNANKSIQYGLTLIADS 179

Query: 173 PMQMFQYIVGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTL 232
           P+++F+Y+  N FINSI+ HT IY+H+KEWQ+   K SR +NI++DR LGIILML+LF+L
Sbjct: 180 PIKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL 239

Query: 233 VSHPGDFLIQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDL 292
            + PGDFLIQISH +I +LY LLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLD 
Sbjct: 240 ATQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDF 299

Query: 293 IEPIVRQVFLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLW 352
           IEP+VRQVFLAIG  GCLGFT+QIALL DLIS++GLH+HCFY+YTKVL NVE +GL+VLW
Sbjct: 300 IEPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLW 359

Query: 353 QVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATL 412
           QVVRGNRYNIL+ R E+HNYMNRQLYLATIFFSAILFL PTTLVYY+VFA LKALT ATL
Sbjct: 360 QVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATL 419

Query: 413 ATLECFRRKLLNFPIEMFLKYIKKGFYEIDCLRVLDIPLQKPLFFIHRNSKLIIFVYKL 471
           +     RRKL+  PIE+ +K + +G +EIDC+++ D+   +  F +H+  K+ + VYK+
Sbjct: 420 SVFHFVRRKLMYLPIEVCIKRLLRGCHEIDCIQIKDVSHHERAFLMHKQQKINVTVYKI 478
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
 gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 760

 Score =  350 bits (889), Expect = 4e-95
 Identities = 77/251 (30%), Positives = 142/251 (55%), Gaps = 2/251 (0%)

Query: 188 SIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLF--TLVSHPGDFLIQISH 245
             +T     R  +    +  K + ++++++D ALG++L+  L   + + H  D L+ ++ 
Sbjct: 255 EHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVAD 314

Query: 246 IIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIG 305
            +  +L  LL+ L G+P GLK+N  L+      F YHI LW +++ L+ P V  +   +G
Sbjct: 315 HVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG 374

Query: 306 AFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRN 365
              CLG T  ++LL+D+I+++  H +CFYVY   L  +++ GL+ LW++ RG ++N+LR 
Sbjct: 375 LSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQ 434

Query: 366 RIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNF 425
           R+++ +Y   QL++ T+ F+ +LFL PTT +YY+VF  L+ L  A    +      + + 
Sbjct: 435 RVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 494

Query: 426 PIEMFLKYIKK 436
           P+      + +
Sbjct: 495 PLYSLGLRLCR 505
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
           component)) [Homo sapiens]
          Length = 583

 Score =  333 bits (847), Expect = 3e-90
 Identities = 77/251 (30%), Positives = 142/251 (55%), Gaps = 2/251 (0%)

Query: 188 SIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLF--TLVSHPGDFLIQISH 245
             +T     R  +    +  K + ++++++D ALG++L+  L   + + H  D L+ ++ 
Sbjct: 257 EHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVAD 316

Query: 246 IIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIG 305
            +  +L  LL+ L G+P GLK+N  L+      F YHI LW +++ L+ P V  +   +G
Sbjct: 317 HVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG 376

Query: 306 AFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRN 365
              CLG T  ++LL+D+I+++  H +CFYVY   L  +++ GL+ LW++ RG ++N+LR 
Sbjct: 377 LSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQ 436

Query: 366 RIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNF 425
           R+++ +Y   QL++ T+ F+ +LFL PTT +YY+VF  L+ L  A    +      + + 
Sbjct: 437 RVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 496

Query: 426 PIEMFLKYIKK 436
           P+      + +
Sbjct: 497 PLYSLGLRLCR 507
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
           sapiens]
          Length = 581

 Score =  333 bits (846), Expect = 4e-90
 Identities = 77/251 (30%), Positives = 142/251 (55%), Gaps = 2/251 (0%)

Query: 188 SIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLF--TLVSHPGDFLIQISH 245
             +T     R  +    +  K + ++++++D ALG++L+  L   + + H  D L+ ++ 
Sbjct: 255 EHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVAD 314

Query: 246 IIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIG 305
            +  +L  LL+ L G+P GLK+N  L+      F YHI LW +++ L+ P V  +   +G
Sbjct: 315 HVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG 374

Query: 306 AFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRN 365
              CLG T  ++LL+D+I+++  H +CFYVY   L  +++ GL+ LW++ RG ++N+LR 
Sbjct: 375 LSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQ 434

Query: 366 RIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNF 425
           R+++ +Y   QL++ T+ F+ +LFL PTT +YY+VF  L+ L  A    +      + + 
Sbjct: 435 RVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 494

Query: 426 PIEMFLKYIKK 436
           P+      + +
Sbjct: 495 PLYSLGLRLCR 505
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
           N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
          Length = 581

 Score =  333 bits (846), Expect = 5e-90
 Identities = 77/251 (30%), Positives = 142/251 (55%), Gaps = 2/251 (0%)

Query: 188 SIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLF--TLVSHPGDFLIQISH 245
             +T     R  +    +  K + ++++++D ALG++L+  L   + + H  D L+ ++ 
Sbjct: 255 EHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVAD 314

Query: 246 IIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIG 305
            +  +L  LL+ L G+P GLK+N  L+      F YHI LW +++ L+ P V  +   +G
Sbjct: 315 HVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVG 374

Query: 306 AFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRN 365
              CLG T  ++LL+D+I+++  H +CFYVY   L  +++ GL+ LW++ RG ++N+LR 
Sbjct: 375 LSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQ 434

Query: 366 RIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNF 425
           R+++ +Y   QL++ T+ F+ +LFL PTT +YY+VF  L+ L  A    +      + + 
Sbjct: 435 RVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSL 494

Query: 426 PIEMFLKYIKK 436
           P+      + +
Sbjct: 495 PLYSLGLRLCR 505
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
           musculus]
          Length = 581

 Score =  322 bits (819), Expect = 6e-87
 Identities = 69/234 (29%), Positives = 134/234 (56%), Gaps = 2/234 (0%)

Query: 205 IYTKGSRLSNIMIDRALGIILMLVLFTL--VSHPGDFLIQISHIIIHQLYSLLKVLEGSP 262
           +  K + L ++++D ALG++L+  L +   +    + L+ ++  +  +L  LL+ L G+P
Sbjct: 272 LMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAP 331

Query: 263 IGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADL 322
            GLK+N  L+      F YHI LW +++ L+ P +  +   +G   CLG T  +++ +D+
Sbjct: 332 AGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDI 391

Query: 323 ISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATI 382
           I+++  H +CFYVY   L  +++ GL+ LW++ RG ++N+LR R+++ +Y   QL++ T+
Sbjct: 392 IALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTL 451

Query: 383 FFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIKK 436
            F+ ++FL PTT +YY+VF  L+ L       +      + + P+      + +
Sbjct: 452 LFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCR 505
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
 gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
           (human) [Mus musculus]
          Length = 581

 Score =  322 bits (817), Expect = 1e-86
 Identities = 69/234 (29%), Positives = 134/234 (56%), Gaps = 2/234 (0%)

Query: 205 IYTKGSRLSNIMIDRALGIILMLVLFTL--VSHPGDFLIQISHIIIHQLYSLLKVLEGSP 262
           +  K + L ++++D ALG++L+  L +   +    + L+ ++  +  +L  LL+ L G+P
Sbjct: 272 LMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAP 331

Query: 263 IGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADL 322
            GLK+N  L+      F YHI LW +++ L+ P +  +   +G   CLG T  +++ +D+
Sbjct: 332 AGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDI 391

Query: 323 ISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATI 382
           I+++  H +CFYVY   L  +++ GL+ LW++ RG ++N+LR R+++ +Y   QL++ T+
Sbjct: 392 IALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTL 451

Query: 383 FFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIKK 436
            F+ ++FL PTT +YY+VF  L+ L       +      + + P+      + +
Sbjct: 452 LFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCR 505
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
           glycosylphosphatidylinositol homolog (S. cerevisiae)
           [Mus musculus]
 dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
          Length = 581

 Score =  318 bits (808), Expect = 1e-85
 Identities = 68/234 (29%), Positives = 133/234 (56%), Gaps = 2/234 (0%)

Query: 205 IYTKGSRLSNIMIDRALGIILMLVLFTL--VSHPGDFLIQISHIIIHQLYSLLKVLEGSP 262
           +  K + L ++++D ALG++L+  L +   +    + L+ ++  +  +L  LL+ L G+P
Sbjct: 272 LMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAP 331

Query: 263 IGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADL 322
            GLK+N  L+      F YHI LW +++ L+ P +  +   +G   CLG T  +++ +D+
Sbjct: 332 AGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDI 391

Query: 323 ISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATI 382
           I+++  H +CFYVY   L  +++ GL+ LW++ RG ++N+LR R+++ +Y   QL++ T+
Sbjct: 392 IALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTL 451

Query: 383 FFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIKK 436
            F+ ++FL PTT +Y +VF  L+ L       +      + + P+      + +
Sbjct: 452 LFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCR 505
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
 pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
 emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
          Length = 680

 Score =  307 bits (780), Expect = 3e-82
 Identities = 59/269 (21%), Positives = 112/269 (40%), Gaps = 5/269 (1%)

Query: 171 NIPMQMFQYIVGNKFINSI-ITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVL 229
           N  ++  Q +    F+  I +      +H +E  +   + S  S + +D  LG ++ L L
Sbjct: 290 NFRIRSCQILYWPIFLEEIDMMSISCVKHAEE--AALQRHSTWSAMAVDLVLGNLIGLGL 347

Query: 230 FTLVSHPGDFLIQISHIIIHQ-LYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWST 288
                    F+   +    +  L S    L G P G KLN  L           I++WST
Sbjct: 348 LFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWST 407

Query: 289 FLDLIEPIVRQVFLAIGAFGC-LGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKG 347
               +   +  +   I   G   G T   A + D+I+   LH    +    ++ + +++ 
Sbjct: 408 LWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQIQA 467

Query: 348 LTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKAL 407
           L  LW++ RG + N LR R++++ Y  +Q  + ++ F+ +L L PTT V+Y+ F      
Sbjct: 468 LAALWRLFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTITSTT 527

Query: 408 TCATLATLECFRRKLLNFPIEMFLKYIKK 436
             +    +E     +   P    + ++ +
Sbjct: 528 INSICMLIEFAISVIHATPYAEVMIWLVR 556
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
          Length = 339

 Score =  306 bits (776), Expect = 6e-82
 Identities = 69/234 (29%), Positives = 134/234 (56%), Gaps = 2/234 (0%)

Query: 205 IYTKGSRLSNIMIDRALGIILMLVLFTL--VSHPGDFLIQISHIIIHQLYSLLKVLEGSP 262
           +  K + L ++++D ALG++L+  L +   +    + L+ ++  +  +L  LL+ L G+P
Sbjct: 30  LMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAP 89

Query: 263 IGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADL 322
            GLK+N  L+      F YHI LW +++ L+ P +  +   +G   CLG T  +++ +D+
Sbjct: 90  AGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDI 149

Query: 323 ISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATI 382
           I+++  H +CFYVY   L  +++ GL+ LW++ RG ++N+LR R+++ +Y   QL++ T+
Sbjct: 150 IALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTL 209

Query: 383 FFSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIKK 436
            F+ ++FL PTT +YY+VF  L+ L       +      + + P+      + +
Sbjct: 210 LFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCR 263
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi1p
           [Saccharomyces cerevisiae]
 sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
 gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
          Length = 609

 Score =  271 bits (687), Expect = 2e-71
 Identities = 71/272 (26%), Positives = 135/272 (49%), Gaps = 17/272 (6%)

Query: 174 MQMFQYIVGNKFINSIITHTVI-YRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTL 232
           +Q    +V     NS  +H+ +  + Y ++  +Y   + +  I+ D + G+IL  +L   
Sbjct: 246 IQNVGSMVEKDNSNSQFSHSYMPSKFYPDYILLY---NTIWLIINDISFGLILGAILI-- 300

Query: 233 VSHPGDFLIQISHIII-----HQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIEL-W 286
                DFL+  SH ++       L ++ + L  +P+G+KLN  L NF  + F + IE  +
Sbjct: 301 --ENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGIKLNAELANFLSELFLWVIEFSY 358

Query: 287 STFLD-LIEPIVRQVFLAIGAFG--CLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNV 343
           +TF+  LI+P      L +  +    +GF++ ++L  D  +I+    + FY  +  L + 
Sbjct: 359 TTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHC 418

Query: 344 EVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFAT 403
           ++  +  L+ +  G + N+LRNRI+ + +   QL L T+ F  ++FL PT + +Y+ +  
Sbjct: 419 QLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTV 478

Query: 404 LKALTCATLATLECFRRKLLNFPIEMFLKYIK 435
           L+ LT       E     + +FP+   L  +K
Sbjct: 479 LRMLTITIEIFSEAVIALINHFPLFALLLRLK 510
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
 pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
 emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
          Length = 287

 Score =  254 bits (643), Expect = 3e-66
 Identities = 71/260 (27%), Positives = 109/260 (41%), Gaps = 20/260 (7%)

Query: 189 IITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPGDFLIQISHIII 248
           I  + V+ R  +    +    S    +++D A  I L  +         +     +  + 
Sbjct: 4   ITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVA 63

Query: 249 HQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFL---------------DLI 293
             L   +  L  +P GLKLN  +N      F YHI LW++ L                 I
Sbjct: 64  DNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFI 123

Query: 294 EPIVRQVFLAIGAFGCL-GFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLW 352
             +    F    A+  + G +   A++ D   I  LH +CF  Y   L  +    LTVLW
Sbjct: 124 GFLRSDAFFRFIAYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLW 183

Query: 353 QVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATL 412
            +VRG ++N LR R +      RQ +LAT  F  +LF+ PT  VY+VVF  L+ L  + L
Sbjct: 184 SLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLR-LAVSAL 242

Query: 413 ATLECFRRKLLNFPIEMFLK 432
            T+  F      +P ++F  
Sbjct: 243 QTVLYF---FATWPFQLFAL 259
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
           - fission yeast (Schizosaccharomyces pombe)
 emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
           protein gpi1. [Schizosaccharomyces pombe]
          Length = 653

 Score =  251 bits (635), Expect = 2e-65
 Identities = 73/259 (28%), Positives = 121/259 (46%), Gaps = 7/259 (2%)

Query: 178 QYIVGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPG 237
           QY+    F  S          YKE+   Y   + L  +  D   GI +   +   +    
Sbjct: 291 QYMKLWVFRKSKRVAI---EDYKEYIRFY---NNLWLVANDMIFGITMSSFILENLHLVV 344

Query: 238 DFLIQIS-HIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPI 296
             +  I+    I  + S++  L  +P GLKLN  +  F +    + I++WS FL    P 
Sbjct: 345 KLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPW 404

Query: 297 VRQVFLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVR 356
              +   +   G  G +  IAL++D +S++ +H H  Y+ +  L N +++ +  L Q+ R
Sbjct: 405 TPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFR 464

Query: 357 GNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLE 416
           G + N+LRNRI+++ Y   QL L TI F+ ++F  PT  V+Y  FA  +      LA  E
Sbjct: 465 GKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICE 524

Query: 417 CFRRKLLNFPIEMFLKYIK 435
                L +FP+ + +  IK
Sbjct: 525 TMLAFLNHFPLFVTMLRIK 543
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
          Length = 517

 Score =  245 bits (620), Expect = 2e-63
 Identities = 72/259 (27%), Positives = 121/259 (45%), Gaps = 7/259 (2%)

Query: 178 QYIVGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTLVSHPG 237
           QY+    F  S          YKE+   Y   + L  +  D   GI +   +   +    
Sbjct: 155 QYMKLWVFRKSKRVAI---EDYKEYIRFY---NNLWLVANDMIFGITMSSFILENLHLVV 208

Query: 238 DFLIQIS-HIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPI 296
             +  I+    I  + S++  L  +P GLKLN  +  F +    + I++WS FL    P 
Sbjct: 209 KLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPW 268

Query: 297 VRQVFLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVR 356
              +   +   G  G +  IAL++D +S++ +H H  Y+ +  + N +++ +  L Q+ R
Sbjct: 269 TPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRMYNWQLRVIYSLLQLFR 328

Query: 357 GNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATLATLE 416
           G + N+LRNRI+++ Y   QL L TI F+ ++F  PT  V+Y  FA  +      LA  E
Sbjct: 329 GKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICE 388

Query: 417 CFRRKLLNFPIEMFLKYIK 435
                L +FP+ + +  IK
Sbjct: 389 TMLAFLNHFPLFVTMLRIK 407
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
          Length = 669

 Score =  233 bits (590), Expect = 4e-60
 Identities = 63/236 (26%), Positives = 116/236 (48%), Gaps = 9/236 (3%)

Query: 211 RLSNIMIDRALGIILMLVLFTLVSHPGDFLIQISHII--IHQLYSLLKVLEGSPIGLKLN 268
           R+ N++ID  LG ++ L+L+  V +   ++ + + I      L S+L  L  +P+G KLN
Sbjct: 359 RIINLIIDIFLGFLIFLLLYFNVINL-HYISEKAQIFYGTSTLTSILGTLLQNPLGFKLN 417

Query: 269 IHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADLISIVGL 328
            +  +F        ++ W  F + I      V   +G    LGF++ ++ + D +  V  
Sbjct: 418 NNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTA 477

Query: 329 HAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAIL 388
           H    Y++ K +  +  K +  L+ +  G ++NIL+ R++ + Y N ++ L TI F+ ++
Sbjct: 478 HVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILI 537

Query: 389 FLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFL------KYIKKGF 438
           FL+PT  V  +VF  +  +    +  L    + +L  P  +F       KYI KGF
Sbjct: 538 FLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGF 593
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana  (fragment)
 emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
          Length = 143

 Score =  156 bits (393), Expect = 6e-37
 Identities = 32/143 (22%), Positives = 56/143 (38%), Gaps = 2/143 (1%)

Query: 215 IMIDRALGIILMLVLFTLVSHPGDFLIQISHIIIHQ-LYSLLKVLEGSPIGLKLNIHLNN 273
           + +D  LG ++ L L         F+   +    +  L S    L G P G KLN  L  
Sbjct: 1   MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 60

Query: 274 FFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGC-LGFTYQIALLADLISIVGLHAHC 332
                    I++WST    +   +  +   I   G   G T   A + D+I+   LH   
Sbjct: 61  VLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMA 120

Query: 333 FYVYTKVLNNVEVKGLTVLWQVV 355
            +    ++ + +++ L  LW++ 
Sbjct: 121 LHWAITLVYSHQIQALAALWRLF 143
CPU time:   141.23 user secs.	    2.65 sys. secs	  143.88 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.315    0.170    0.506 

Gapped
Lambda     K      H
   0.270   0.0597    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 328297406
Number of Sequences: 887402
Number of extensions: 17423262
Number of successful extensions: 78471
Number of sequences better than 10.0: 211
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 193
Number of HSP's that attempted gapping in prelim test: 78161
Number of HSP's gapped (non-prelim): 395
length of query: 473
length of database: 277,845,442
effective HSP length: 52
effective length of query: 421
effective length of database: 231,700,538
effective search space: 97545926498
effective search space used: 97545926498
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.7 bits)
S2: 75 (33.3 bits)