Sequences with E-value BETTER than threshold (0.002)
emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 683 0.0
emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 652 0.0
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 374 e-102
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 357 3e-97
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 356 4e-97
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 356 5e-97
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 348 8e-95
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 348 9e-95
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 345 1e-93
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 331 1e-89
sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 327 4e-88
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 319 7e-86
ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 303 4e-81
ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 253 4e-66
ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 245 1e-63
emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 234 2e-60
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 152 1e-35
Sequences with E-value WORSE than threshold
dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:13331... 35 1.9
Alignments
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
Length = 481
Score = 683 bits (1744), Expect = 0.0
Identities = 481/481 (100%), Positives = 481/481 (100%)
Query: 1 MSIKIYLPVNYYYLNKSTNLYGQVQINEDNVVSYYVLEASDLEFSDKAINHEKGNLRFLG 60
MSIKIYLPVNYYYLNKSTNLYGQVQINEDNVVSYYVLEASDLEFSDKAINHEKGNLRFLG
Sbjct: 1 MSIKIYLPVNYYYLNKSTNLYGQVQINEDNVVSYYVLEASDLEFSDKAINHEKGNLRFLG 60
Query: 61 SILNEDFNADQNQFLKFNMRLCFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNL 120
SILNEDFNADQNQFLKFNMRLCFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNL
Sbjct: 61 SILNEDFNADQNQFLKFNMRLCFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNL 120
Query: 121 FVSGESENRRSLEYNDNDSTDCDFLELSRLNQPTADNQNKANNNANKSIQYGLTLIADSP 180
FVSGESENRRSLEYNDNDSTDCDFLELSRLNQPTADNQNKANNNANKSIQYGLTLIADSP
Sbjct: 121 FVSGESENRRSLEYNDNDSTDCDFLELSRLNQPTADNQNKANNNANKSIQYGLTLIADSP 180
Query: 181 IKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLA 240
IKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLA
Sbjct: 181 IKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLA 240
Query: 241 TQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFI 300
TQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFI
Sbjct: 241 TQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFI 300
Query: 301 EPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQ 360
EPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQ
Sbjct: 301 EPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQ 360
Query: 361 VVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLS 420
VVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLS
Sbjct: 361 VVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLS 420
Query: 421 VFHFVRRKLMYLPIEVCIKRLLRGCHEIDCIQIKDVSHHERAFLMHKQQKINVTVYKITT 480
VFHFVRRKLMYLPIEVCIKRLLRGCHEIDCIQIKDVSHHERAFLMHKQQKINVTVYKITT
Sbjct: 421 VFHFVRRKLMYLPIEVCIKRLLRGCHEIDCIQIKDVSHHERAFLMHKQQKINVTVYKITT 480
Query: 481 L 481
L
Sbjct: 481 L 481
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
Length = 473
Score = 652 bits (1666), Expect = 0.0
Identities = 281/479 (58%), Positives = 360/479 (74%), Gaps = 9/479 (1%)
Query: 1 MSIKIYLPVNYYYLNKSTNLYGQVQINEDNVVSYYVLEASDLEFSDKAINHEKGNLRFLG 60
MSIKI+LP Y+YL ++ NLYGQ+ I+EDNVV YYV++A + ++ + +RFLG
Sbjct: 1 MSIKIFLPTEYFYLKRACNLYGQLHISEDNVVVYYVVDA-----DNNKLDSDTNKMRFLG 55
Query: 61 SILNEDFNADQNQFLKFNMRLCFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNL 120
SIL D AD N ++ LCFTY++ N++L++ GITPE L +K+ILYDKQ VR+L
Sbjct: 56 SILCSDSYADSNYCRSHDVVLCFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSL 115
Query: 121 FVSGESENRRSLEYNDNDSTD-CDFLELSRLNQPTADNQNKANNNANKSIQYGLTLIADS 179
+ + N + +N D CDF L++L QP D K+N+ Q L L+A+
Sbjct: 116 VI--KDNNTLAHWTRENQINDECDFYMLAQLVQPNPDTCRKSNDLWFYGSQ-SLCLLANI 172
Query: 180 PIKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL 239
P+++F+Y+ N FINSI+ HT IY+H+KEWQ+ K SR +NI++DR LGIILML+LF+L
Sbjct: 173 PMQMFQYIVGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTL 232
Query: 240 ATQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDF 299
+ PGDFLIQISH +I +LY LLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLD
Sbjct: 233 VSHPGDFLIQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDL 292
Query: 300 IEPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLW 359
IEP+VRQVFLAIG GCLGFT+QIALL DLIS++GLH+HCFY+YTKVL NVE +GL+VLW
Sbjct: 293 IEPIVRQVFLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLW 352
Query: 360 QVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATL 419
QVVRGNRYNIL+ R E+HNYMNRQLYLATIFFSAILFL PTTLVYY+VFA LKALT ATL
Sbjct: 353 QVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATL 412
Query: 420 SVFHFVRRKLMYLPIEVCIKRLLRGCHEIDCIQIKDVSHHERAFLMHKQQKINVTVYKI 478
+ RRKL+ PIE+ +K + +G +EIDC+++ D+ + F +H+ K+ + VYK+
Sbjct: 413 ATLECFRRKLLNFPIEMFLKYIKKGFYEIDCLRVLDIPLQKPLFFIHRNSKLIIFVYKL 471
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
sapiens]
gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
sapiens]
Length = 760
Score = 374 bits (952), Expect = e-102
Identities = 84/242 (34%), Positives = 139/242 (56%), Gaps = 2/242 (0%)
Query: 204 KHFKEWQTACDKRSRPANIVLDRILGIILMLILF--SLATQPGDFLIQISHYVIDELYGL 261
+ + K + A+++LD LG++L+ L S D L+ ++ +V +EL L
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323
Query: 262 LKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTF 321
L+ L G+P GLK+N L+ F YHI LW +++ + P V + +G+ CLG T
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV 383
Query: 322 QIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMN 381
++LL D+I+++ H +CFY+Y LY ++ GLS LW++ RG ++N+L+ R +S +Y
Sbjct: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443
Query: 382 RQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRL 441
QL++ T+ F+ +LFLLPTT +YY+VF L+ L A + H + + LP+ RL
Sbjct: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503
Query: 442 LR 443
R
Sbjct: 504 CR 505
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
component)) [Homo sapiens]
Length = 583
Score = 357 bits (907), Expect = 3e-97
Identities = 84/242 (34%), Positives = 139/242 (56%), Gaps = 2/242 (0%)
Query: 204 KHFKEWQTACDKRSRPANIVLDRILGIILMLILF--SLATQPGDFLIQISHYVIDELYGL 261
+ + K + A+++LD LG++L+ L S D L+ ++ +V +EL L
Sbjct: 266 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 325
Query: 262 LKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTF 321
L+ L G+P GLK+N L+ F YHI LW +++ + P V + +G+ CLG T
Sbjct: 326 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV 385
Query: 322 QIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMN 381
++LL D+I+++ H +CFY+Y LY ++ GLS LW++ RG ++N+L+ R +S +Y
Sbjct: 386 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 445
Query: 382 RQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRL 441
QL++ T+ F+ +LFLLPTT +YY+VF L+ L A + H + + LP+ RL
Sbjct: 446 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 505
Query: 442 LR 443
R
Sbjct: 506 CR 507
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
sapiens]
Length = 581
Score = 356 bits (906), Expect = 4e-97
Identities = 84/242 (34%), Positives = 139/242 (56%), Gaps = 2/242 (0%)
Query: 204 KHFKEWQTACDKRSRPANIVLDRILGIILMLILF--SLATQPGDFLIQISHYVIDELYGL 261
+ + K + A+++LD LG++L+ L S D L+ ++ +V +EL L
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323
Query: 262 LKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTF 321
L+ L G+P GLK+N L+ F YHI LW +++ + P V + +G+ CLG T
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV 383
Query: 322 QIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMN 381
++LL D+I+++ H +CFY+Y LY ++ GLS LW++ RG ++N+L+ R +S +Y
Sbjct: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443
Query: 382 RQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRL 441
QL++ T+ F+ +LFLLPTT +YY+VF L+ L A + H + + LP+ RL
Sbjct: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503
Query: 442 LR 443
R
Sbjct: 504 CR 505
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
N-acetylglucosaminyl transferase component Gpi1 [Homo
sapiens]
gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
sapiens]
dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
Length = 581
Score = 356 bits (905), Expect = 5e-97
Identities = 84/242 (34%), Positives = 139/242 (56%), Gaps = 2/242 (0%)
Query: 204 KHFKEWQTACDKRSRPANIVLDRILGIILMLILF--SLATQPGDFLIQISHYVIDELYGL 261
+ + K + A+++LD LG++L+ L S D L+ ++ +V +EL L
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323
Query: 262 LKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTF 321
L+ L G+P GLK+N L+ F YHI LW +++ + P V + +G+ CLG T
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV 383
Query: 322 QIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMN 381
++LL D+I+++ H +CFY+Y LY ++ GLS LW++ RG ++N+L+ R +S +Y
Sbjct: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443
Query: 382 RQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRL 441
QL++ T+ F+ +LFLLPTT +YY+VF L+ L A + H + + LP+ RL
Sbjct: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503
Query: 442 LR 443
R
Sbjct: 504 CR 505
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
(human) [Mus musculus]
Length = 581
Score = 348 bits (886), Expect = 8e-95
Identities = 79/250 (31%), Positives = 138/250 (54%), Gaps = 5/250 (2%)
Query: 199 HTTIY---KHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL--ATQPGDFLIQISHY 253
H + + + K + +++LD LG++L+ L S Q + L+ ++
Sbjct: 256 HLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADR 315
Query: 254 VIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGM 313
V +EL LL+ L G+P GLK+N L+ F YHI LW +++ + P + + +G+
Sbjct: 316 VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGL 375
Query: 314 IGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGR 373
CLG T +++ D+I+++ H +CFY+Y LY ++ GLS LW++ RG ++N+L+ R
Sbjct: 376 SACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQR 435
Query: 374 TESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLP 433
+S +Y QL++ T+ F+ ++FLLPTT +YY+VF L+ L + H + + LP
Sbjct: 436 VDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLP 495
Query: 434 IEVCIKRLLR 443
+ RL R
Sbjct: 496 LYSLGLRLCR 505
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
musculus]
Length = 581
Score = 348 bits (886), Expect = 9e-95
Identities = 79/250 (31%), Positives = 138/250 (54%), Gaps = 5/250 (2%)
Query: 199 HTTIYKHFKE---WQTACDKRSRPANIVLDRILGIILMLILFSL--ATQPGDFLIQISHY 253
H + ++ K + +++LD LG++L+ L S Q + L+ ++
Sbjct: 256 HLSFIFSTEKAQSPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADR 315
Query: 254 VIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGM 313
V +EL LL+ L G+P GLK+N L+ F YHI LW +++ + P + + +G+
Sbjct: 316 VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGL 375
Query: 314 IGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGR 373
CLG T +++ D+I+++ H +CFY+Y LY ++ GLS LW++ RG ++N+L+ R
Sbjct: 376 SACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQR 435
Query: 374 TESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLP 433
+S +Y QL++ T+ F+ ++FLLPTT +YY+VF L+ L + H + + LP
Sbjct: 436 VDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLP 495
Query: 434 IEVCIKRLLR 443
+ RL R
Sbjct: 496 LYSLGLRLCR 505
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
glycosylphosphatidylinositol homolog (S. cerevisiae)
[Mus musculus]
dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
Length = 581
Score = 345 bits (877), Expect = 1e-93
Identities = 78/250 (31%), Positives = 137/250 (54%), Gaps = 5/250 (2%)
Query: 199 HTTIY---KHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL--ATQPGDFLIQISHY 253
H + + + K + +++LD LG++L+ L S Q + L+ ++
Sbjct: 256 HLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADR 315
Query: 254 VIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGM 313
V +EL LL+ L G+P GLK+N L+ F YHI LW +++ + P + + +G+
Sbjct: 316 VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGL 375
Query: 314 IGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGR 373
CLG T +++ D+I+++ H +CFY+Y LY ++ GLS LW++ RG ++N+L+ R
Sbjct: 376 SACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQR 435
Query: 374 TESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLP 433
+S +Y QL++ T+ F+ ++FLLPTT +Y +VF L+ L + H + + LP
Sbjct: 436 VDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLP 495
Query: 434 IEVCIKRLLR 443
+ RL R
Sbjct: 496 LYSLGLRLCR 505
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
Length = 339
Score = 331 bits (842), Expect = 1e-89
Identities = 79/250 (31%), Positives = 138/250 (54%), Gaps = 5/250 (2%)
Query: 199 HTTIY---KHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL--ATQPGDFLIQISHY 253
H + + + K + +++LD LG++L+ L S Q + L+ ++
Sbjct: 14 HLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADR 73
Query: 254 VIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGM 313
V +EL LL+ L G+P GLK+N L+ F YHI LW +++ + P + + +G+
Sbjct: 74 VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGL 133
Query: 314 IGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGR 373
CLG T +++ D+I+++ H +CFY+Y LY ++ GLS LW++ RG ++N+L+ R
Sbjct: 134 SACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQR 193
Query: 374 TESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLP 433
+S +Y QL++ T+ F+ ++FLLPTT +YY+VF L+ L + H + + LP
Sbjct: 194 VDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLP 253
Query: 434 IEVCIKRLLR 443
+ RL R
Sbjct: 254 LYSLGLRLCR 263
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
- fission yeast (Schizosaccharomyces pombe)
emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
protein gpi1. [Schizosaccharomyces pombe]
Length = 653
Score = 327 bits (830), Expect = 4e-88
Identities = 70/259 (27%), Positives = 122/259 (47%), Gaps = 7/259 (2%)
Query: 185 EYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPAN-IVLDRILGIILMLILFSLATQP 243
+YM VF S V + +KE+ + AN ++ + ++ L +
Sbjct: 291 QYMKLWVFRKSKRVAI---EDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLI 347
Query: 244 GDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPL 303
+ + Y I + ++ L +P GLKLN + F + + I++WS FL P
Sbjct: 348 ENITFE---YAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPW 404
Query: 304 VRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVR 363
+ + + G G + IAL+ D +SV+ +H H Y+ + LYN + R + L Q+ R
Sbjct: 405 TPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFR 464
Query: 364 GNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFH 423
G + N+L+ R +S+ Y QL L TI F+ ++F LPT V+Y FA + L++
Sbjct: 465 GKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICE 524
Query: 424 FVRRKLMYLPIEVCIKRLL 442
+ L + P+ V + R+
Sbjct: 525 TMLAFLNHFPLFVTMLRIK 543
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
Length = 517
Score = 319 bits (810), Expect = 7e-86
Identities = 69/259 (26%), Positives = 122/259 (46%), Gaps = 7/259 (2%)
Query: 185 EYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPAN-IVLDRILGIILMLILFSLATQP 243
+YM VF S V + +KE+ + AN ++ + ++ L +
Sbjct: 155 QYMKLWVFRKSKRVAI---EDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLI 211
Query: 244 GDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPL 303
+ + Y I + ++ L +P GLKLN + F + + I++WS FL P
Sbjct: 212 ENITFE---YAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPW 268
Query: 304 VRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVR 363
+ + + G G + IAL+ D +SV+ +H H Y+ + +YN + R + L Q+ R
Sbjct: 269 TPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRMYNWQLRVIYSLLQLFR 328
Query: 364 GNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFH 423
G + N+L+ R +S+ Y QL L TI F+ ++F LPT V+Y FA + L++
Sbjct: 329 GKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICE 388
Query: 424 FVRRKLMYLPIEVCIKRLL 442
+ L + P+ V + R+
Sbjct: 389 TMLAFLNHFPLFVTMLRIK 407
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
Length = 680
Score = 303 bits (769), Expect = 4e-81
Identities = 68/272 (25%), Positives = 121/272 (44%), Gaps = 11/272 (4%)
Query: 178 DSPIKIFEYMAENVFINSI-MVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLIL 236
+ I+ + + +F+ I M+ + KH +E A + S + + +D +LG ++ L L
Sbjct: 290 NFRIRSCQILYWPIFLEEIDMMSISCVKHAEE--AALQRHSTWSAMAVDLVLGNLIGLGL 347
Query: 237 FSLATQPGDFLIQISHYVIDE-LYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWST 295
F+ + + L L G P G KLN L I++WST
Sbjct: 348 LFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWST 407
Query: 296 FLDFIEPLVRQVFLAIGMIGCLGFTF----QIALLVDLISVIGLHSHCFYIYTKVLYNVE 351
F+ +F I +I LG TF A ++D+I+ LH + ++Y+ +
Sbjct: 408 LWVFM---ASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQ 464
Query: 352 RRGLSVLWQVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAAL 411
+ L+ LW++ RG + N L+ R +S+ Y +Q + ++ F+ +L LLPTT V+YI F
Sbjct: 465 IQALAALWRLFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTIT 524
Query: 412 KALTFATLSVFHFVRRKLMYLPIEVCIKRLLR 443
+ + F + P + L+R
Sbjct: 525 STTINSICMLIEFAISVIHATPYAEVMIWLVR 556
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
Length = 287
Score = 253 bits (642), Expect = 4e-66
Identities = 70/248 (28%), Positives = 106/248 (42%), Gaps = 20/248 (8%)
Query: 196 IMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLATQPGDFLIQISHYVI 255
I + + + + S ++LD I L I + + V
Sbjct: 4 ITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVA 63
Query: 256 DELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELW------------------STFL 297
D L G + L +P GLKLN +N F YHI LW S F+
Sbjct: 64 DNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFI 123
Query: 298 DFIEPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSV 357
F+ F+A +IG G + A++ D + LH +CF Y L + L+V
Sbjct: 124 GFLRSDAFFRFIAYSLIG--GISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTV 181
Query: 358 LWQVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFA 417
LW +VRG ++N L+ R ++ RQ +LAT F +LF+LPT VY++VF L+ A
Sbjct: 182 LWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSA 241
Query: 418 TLSVFHFV 425
+V +F
Sbjct: 242 LQTVLYFF 249
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
N-acetylglucoaminylphosphatidylinositol, the first
intermediate in synthesis of
glycosylphosphatidylinositol (GPI) anchors; Gpi1p
[Saccharomyces cerevisiae]
sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
cerevisiae)
emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
Length = 609
Score = 245 bits (620), Expect = 1e-63
Identities = 65/235 (27%), Positives = 119/235 (49%), Gaps = 13/235 (5%)
Query: 217 SRPANIVLDRILGIILMLILFSLATQPGDFLIQISHYVI-----DELYGLLKVLEGSPIG 271
+ I+ D G LIL ++ + DFL+ SH V+ D L + + L +P+G
Sbjct: 280 NTIWLIINDISFG----LILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLG 335
Query: 272 LKLNIHLNNFFLDCFKYHIEL-WSTFLD-FIEP--LVRQVFLAIGMIGCLGFTFQIALLV 327
+KLN L NF + F + IE ++TF+ I+P L + L I M+ +GF+F ++L +
Sbjct: 336 IKLNAELANFLSELFLWVIEFSYTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAI 395
Query: 328 DLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMNRQLYLA 387
D +++ + FY + LY+ + ++ L+ + G + N+L+ R + + + QL L
Sbjct: 396 DFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLG 455
Query: 388 TIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRLL 442
T+ F ++FL PT + +Y+ + L+ LT V + + P+ + RL
Sbjct: 456 TLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLK 510
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
Length = 669
Score = 234 bits (593), Expect = 2e-60
Identities = 55/262 (20%), Positives = 113/262 (42%), Gaps = 23/262 (8%)
Query: 201 TIYKHFKEWQTACDKRSRPANIVLDRILGIIL-----------MLILFSLATQPGDFLIQ 249
+ K+ + R +++ I+ I L ++ L ++ + F
Sbjct: 338 SFMKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIF--- 394
Query: 250 ISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFL 309
Y L +L L +P+G KLN + +F ++ W F + I V
Sbjct: 395 ---YGTSTLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLN 451
Query: 310 AIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNI 369
+G LGF+F ++ ++D + + H Y++ K + + + + L+ + G ++NI
Sbjct: 452 FVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNI 511
Query: 370 LKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKL 429
LK R +++ Y N ++ L TI F+ ++FL PT V +VF + + + + + + +
Sbjct: 512 LKLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKII 571
Query: 430 MYLPIEV------CIKRLLRGC 445
+Y P + C K + +G
Sbjct: 572 LYTPFYIFFIQPNCNKYISKGF 593
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana (fragment)
emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
Length = 143
Score = 152 bits (382), Expect = 1e-35
Identities = 36/146 (24%), Positives = 62/146 (41%), Gaps = 8/146 (5%)
Query: 222 IVLDRILGIILMLILFSLATQPGDFLIQISHYVIDE-LYGLLKVLEGSPIGLKLNIHLNN 280
+ +D +LG ++ L L F+ + + L L G P G KLN L
Sbjct: 1 MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 60
Query: 281 FFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTF----QIALLVDLISVIGLH 336
I++WST F+ +F I +I LG TF A ++D+I+ LH
Sbjct: 61 VLGMVSLNVIQIWSTLWVFM---ASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLH 117
Query: 337 SHCFYIYTKVLYNVERRGLSVLWQVV 362
+ ++Y+ + + L+ LW++
Sbjct: 118 IMALHWAITLVYSHQIQALAALWRLF 143
>dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:1333114,
evidence:ISS~phosphatidylinositol glycan, class
Q~putative [Mus musculus]
Length = 378
Score = 35.4 bits (81), Expect = 1.9
Identities = 11/59 (18%), Positives = 25/59 (41%), Gaps = 5/59 (8%)
Query: 199 HTTIY---KHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL--ATQPGDFLIQISH 252
H + + + K + +++LD LG++L+ L S Q + L+ ++
Sbjct: 256 HLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVAD 314
CPU time: 146.30 user secs. 2.48 sys. secs 148.78 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.315 0.168 0.502
Gapped
Lambda K H
0.270 0.0590 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 321734339
Number of Sequences: 887402
Number of extensions: 16633612
Number of successful extensions: 82953
Number of sequences better than 10.0: 162
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 145
Number of HSP's that attempted gapping in prelim test: 82741
Number of HSP's gapped (non-prelim): 304
length of query: 481
length of database: 277,845,442
effective HSP length: 55
effective length of query: 426
effective length of database: 229,038,332
effective search space: 97570329432
effective search space used: 97570329432
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.7 bits)
S2: 75 (33.3 bits)