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GPI Anchor Biosynthesis Report: CAC10056.1 (PIG-Q family, Drosophila melanogaster)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (481 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 18 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 683 0.0
emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 652 0.0
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 374 e-102
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 357 3e-97
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 356 4e-97
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 356 5e-97
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 348 8e-95
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 348 9e-95
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 345 1e-93
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 331 1e-89
sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 327 4e-88
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 319 7e-86
ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 303 4e-81
ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 253 4e-66
ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 245 1e-63
emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 234 2e-60
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 152 1e-35
Sequences with E-value WORSE than threshold

dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:13331... 35 1.9
Alignments
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
          Length = 481

 Score =  683 bits (1744), Expect = 0.0
 Identities = 481/481 (100%), Positives = 481/481 (100%)

Query: 1   MSIKIYLPVNYYYLNKSTNLYGQVQINEDNVVSYYVLEASDLEFSDKAINHEKGNLRFLG 60
           MSIKIYLPVNYYYLNKSTNLYGQVQINEDNVVSYYVLEASDLEFSDKAINHEKGNLRFLG
Sbjct: 1   MSIKIYLPVNYYYLNKSTNLYGQVQINEDNVVSYYVLEASDLEFSDKAINHEKGNLRFLG 60

Query: 61  SILNEDFNADQNQFLKFNMRLCFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNL 120
           SILNEDFNADQNQFLKFNMRLCFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNL
Sbjct: 61  SILNEDFNADQNQFLKFNMRLCFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNL 120

Query: 121 FVSGESENRRSLEYNDNDSTDCDFLELSRLNQPTADNQNKANNNANKSIQYGLTLIADSP 180
           FVSGESENRRSLEYNDNDSTDCDFLELSRLNQPTADNQNKANNNANKSIQYGLTLIADSP
Sbjct: 121 FVSGESENRRSLEYNDNDSTDCDFLELSRLNQPTADNQNKANNNANKSIQYGLTLIADSP 180

Query: 181 IKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLA 240
           IKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLA
Sbjct: 181 IKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLA 240

Query: 241 TQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFI 300
           TQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFI
Sbjct: 241 TQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFI 300

Query: 301 EPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQ 360
           EPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQ
Sbjct: 301 EPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQ 360

Query: 361 VVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLS 420
           VVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLS
Sbjct: 361 VVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLS 420

Query: 421 VFHFVRRKLMYLPIEVCIKRLLRGCHEIDCIQIKDVSHHERAFLMHKQQKINVTVYKITT 480
           VFHFVRRKLMYLPIEVCIKRLLRGCHEIDCIQIKDVSHHERAFLMHKQQKINVTVYKITT
Sbjct: 421 VFHFVRRKLMYLPIEVCIKRLLRGCHEIDCIQIKDVSHHERAFLMHKQQKINVTVYKITT 480

Query: 481 L 481
           L
Sbjct: 481 L 481
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
          Length = 473

 Score =  652 bits (1666), Expect = 0.0
 Identities = 281/479 (58%), Positives = 360/479 (74%), Gaps = 9/479 (1%)

Query: 1   MSIKIYLPVNYYYLNKSTNLYGQVQINEDNVVSYYVLEASDLEFSDKAINHEKGNLRFLG 60
           MSIKI+LP  Y+YL ++ NLYGQ+ I+EDNVV YYV++A      +  ++ +   +RFLG
Sbjct: 1   MSIKIFLPTEYFYLKRACNLYGQLHISEDNVVVYYVVDA-----DNNKLDSDTNKMRFLG 55

Query: 61  SILNEDFNADQNQFLKFNMRLCFTYNSSQANITLVYLGITPEYLKHIKVILYDKQMVRNL 120
           SIL  D  AD N     ++ LCFTY++   N++L++ GITPE L  +K+ILYDKQ VR+L
Sbjct: 56  SILCSDSYADSNYCRSHDVVLCFTYSNDAPNVSLMFTGITPENLNQVKLILYDKQTVRSL 115

Query: 121 FVSGESENRRSLEYNDNDSTD-CDFLELSRLNQPTADNQNKANNNANKSIQYGLTLIADS 179
            +  +  N  +    +N   D CDF  L++L QP  D   K+N+      Q  L L+A+ 
Sbjct: 116 VI--KDNNTLAHWTRENQINDECDFYMLAQLVQPNPDTCRKSNDLWFYGSQ-SLCLLANI 172

Query: 180 PIKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL 239
           P+++F+Y+  N FINSI+ HT IY+H+KEWQ+   K SR +NI++DR LGIILML+LF+L
Sbjct: 173 PMQMFQYIVGNKFINSIITHTVIYRHYKEWQSIYTKGSRLSNIMIDRALGIILMLVLFTL 232

Query: 240 ATQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDF 299
            + PGDFLIQISH +I +LY LLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLD 
Sbjct: 233 VSHPGDFLIQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDL 292

Query: 300 IEPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLW 359
           IEP+VRQVFLAIG  GCLGFT+QIALL DLIS++GLH+HCFY+YTKVL NVE +GL+VLW
Sbjct: 293 IEPIVRQVFLAIGAFGCLGFTYQIALLADLISIVGLHAHCFYVYTKVLNNVEVKGLTVLW 352

Query: 360 QVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATL 419
           QVVRGNRYNIL+ R E+HNYMNRQLYLATIFFSAILFL PTTLVYY+VFA LKALT ATL
Sbjct: 353 QVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTTLVYYVVFATLKALTCATL 412

Query: 420 SVFHFVRRKLMYLPIEVCIKRLLRGCHEIDCIQIKDVSHHERAFLMHKQQKINVTVYKI 478
           +     RRKL+  PIE+ +K + +G +EIDC+++ D+   +  F +H+  K+ + VYK+
Sbjct: 413 ATLECFRRKLLNFPIEMFLKYIKKGFYEIDCLRVLDIPLQKPLFFIHRNSKLIIFVYKL 471
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
 gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 760

 Score =  374 bits (952), Expect = e-102
 Identities = 84/242 (34%), Positives = 139/242 (56%), Gaps = 2/242 (0%)

Query: 204 KHFKEWQTACDKRSRPANIVLDRILGIILMLILF--SLATQPGDFLIQISHYVIDELYGL 261
           +  +       K +  A+++LD  LG++L+  L   S      D L+ ++ +V +EL  L
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323

Query: 262 LKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTF 321
           L+ L G+P GLK+N  L+      F YHI LW +++  + P V  +   +G+  CLG T 
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV 383

Query: 322 QIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMN 381
            ++LL D+I+++  H +CFY+Y   LY ++  GLS LW++ RG ++N+L+ R +S +Y  
Sbjct: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443

Query: 382 RQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRL 441
            QL++ T+ F+ +LFLLPTT +YY+VF  L+ L  A   + H +   +  LP+     RL
Sbjct: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503

Query: 442 LR 443
            R
Sbjct: 504 CR 505
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
           component)) [Homo sapiens]
          Length = 583

 Score =  357 bits (907), Expect = 3e-97
 Identities = 84/242 (34%), Positives = 139/242 (56%), Gaps = 2/242 (0%)

Query: 204 KHFKEWQTACDKRSRPANIVLDRILGIILMLILF--SLATQPGDFLIQISHYVIDELYGL 261
           +  +       K +  A+++LD  LG++L+  L   S      D L+ ++ +V +EL  L
Sbjct: 266 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 325

Query: 262 LKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTF 321
           L+ L G+P GLK+N  L+      F YHI LW +++  + P V  +   +G+  CLG T 
Sbjct: 326 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV 385

Query: 322 QIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMN 381
            ++LL D+I+++  H +CFY+Y   LY ++  GLS LW++ RG ++N+L+ R +S +Y  
Sbjct: 386 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 445

Query: 382 RQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRL 441
            QL++ T+ F+ +LFLLPTT +YY+VF  L+ L  A   + H +   +  LP+     RL
Sbjct: 446 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 505

Query: 442 LR 443
            R
Sbjct: 506 CR 507
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
           sapiens]
          Length = 581

 Score =  356 bits (906), Expect = 4e-97
 Identities = 84/242 (34%), Positives = 139/242 (56%), Gaps = 2/242 (0%)

Query: 204 KHFKEWQTACDKRSRPANIVLDRILGIILMLILF--SLATQPGDFLIQISHYVIDELYGL 261
           +  +       K +  A+++LD  LG++L+  L   S      D L+ ++ +V +EL  L
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323

Query: 262 LKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTF 321
           L+ L G+P GLK+N  L+      F YHI LW +++  + P V  +   +G+  CLG T 
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV 383

Query: 322 QIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMN 381
            ++LL D+I+++  H +CFY+Y   LY ++  GLS LW++ RG ++N+L+ R +S +Y  
Sbjct: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443

Query: 382 RQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRL 441
            QL++ T+ F+ +LFLLPTT +YY+VF  L+ L  A   + H +   +  LP+     RL
Sbjct: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503

Query: 442 LR 443
            R
Sbjct: 504 CR 505
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
           N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
          Length = 581

 Score =  356 bits (905), Expect = 5e-97
 Identities = 84/242 (34%), Positives = 139/242 (56%), Gaps = 2/242 (0%)

Query: 204 KHFKEWQTACDKRSRPANIVLDRILGIILMLILF--SLATQPGDFLIQISHYVIDELYGL 261
           +  +       K +  A+++LD  LG++L+  L   S      D L+ ++ +V +EL  L
Sbjct: 264 RKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHL 323

Query: 262 LKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTF 321
           L+ L G+P GLK+N  L+      F YHI LW +++  + P V  +   +G+  CLG T 
Sbjct: 324 LQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSACLGLTV 383

Query: 322 QIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMN 381
            ++LL D+I+++  H +CFY+Y   LY ++  GLS LW++ RG ++N+L+ R +S +Y  
Sbjct: 384 ALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDSCSYDL 443

Query: 382 RQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRL 441
            QL++ T+ F+ +LFLLPTT +YY+VF  L+ L  A   + H +   +  LP+     RL
Sbjct: 444 DQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRL 503

Query: 442 LR 443
            R
Sbjct: 504 CR 505
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
 gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
           (human) [Mus musculus]
          Length = 581

 Score =  348 bits (886), Expect = 8e-95
 Identities = 79/250 (31%), Positives = 138/250 (54%), Gaps = 5/250 (2%)

Query: 199 HTTIY---KHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL--ATQPGDFLIQISHY 253
           H +     +  +       K +   +++LD  LG++L+  L S     Q  + L+ ++  
Sbjct: 256 HLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADR 315

Query: 254 VIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGM 313
           V +EL  LL+ L G+P GLK+N  L+      F YHI LW +++  + P +  +   +G+
Sbjct: 316 VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGL 375

Query: 314 IGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGR 373
             CLG T  +++  D+I+++  H +CFY+Y   LY ++  GLS LW++ RG ++N+L+ R
Sbjct: 376 SACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQR 435

Query: 374 TESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLP 433
            +S +Y   QL++ T+ F+ ++FLLPTT +YY+VF  L+ L      + H +   +  LP
Sbjct: 436 VDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLP 495

Query: 434 IEVCIKRLLR 443
           +     RL R
Sbjct: 496 LYSLGLRLCR 505
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
           musculus]
          Length = 581

 Score =  348 bits (886), Expect = 9e-95
 Identities = 79/250 (31%), Positives = 138/250 (54%), Gaps = 5/250 (2%)

Query: 199 HTTIYKHFKE---WQTACDKRSRPANIVLDRILGIILMLILFSL--ATQPGDFLIQISHY 253
           H +     ++         K +   +++LD  LG++L+  L S     Q  + L+ ++  
Sbjct: 256 HLSFIFSTEKAQSPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADR 315

Query: 254 VIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGM 313
           V +EL  LL+ L G+P GLK+N  L+      F YHI LW +++  + P +  +   +G+
Sbjct: 316 VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGL 375

Query: 314 IGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGR 373
             CLG T  +++  D+I+++  H +CFY+Y   LY ++  GLS LW++ RG ++N+L+ R
Sbjct: 376 SACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQR 435

Query: 374 TESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLP 433
            +S +Y   QL++ T+ F+ ++FLLPTT +YY+VF  L+ L      + H +   +  LP
Sbjct: 436 VDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLP 495

Query: 434 IEVCIKRLLR 443
           +     RL R
Sbjct: 496 LYSLGLRLCR 505
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
           glycosylphosphatidylinositol homolog (S. cerevisiae)
           [Mus musculus]
 dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
          Length = 581

 Score =  345 bits (877), Expect = 1e-93
 Identities = 78/250 (31%), Positives = 137/250 (54%), Gaps = 5/250 (2%)

Query: 199 HTTIY---KHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL--ATQPGDFLIQISHY 253
           H +     +  +       K +   +++LD  LG++L+  L S     Q  + L+ ++  
Sbjct: 256 HLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADR 315

Query: 254 VIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGM 313
           V +EL  LL+ L G+P GLK+N  L+      F YHI LW +++  + P +  +   +G+
Sbjct: 316 VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGL 375

Query: 314 IGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGR 373
             CLG T  +++  D+I+++  H +CFY+Y   LY ++  GLS LW++ RG ++N+L+ R
Sbjct: 376 SACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQR 435

Query: 374 TESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLP 433
            +S +Y   QL++ T+ F+ ++FLLPTT +Y +VF  L+ L      + H +   +  LP
Sbjct: 436 VDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLP 495

Query: 434 IEVCIKRLLR 443
           +     RL R
Sbjct: 496 LYSLGLRLCR 505
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
          Length = 339

 Score =  331 bits (842), Expect = 1e-89
 Identities = 79/250 (31%), Positives = 138/250 (54%), Gaps = 5/250 (2%)

Query: 199 HTTIY---KHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL--ATQPGDFLIQISHY 253
           H +     +  +       K +   +++LD  LG++L+  L S     Q  + L+ ++  
Sbjct: 14  HLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADR 73

Query: 254 VIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGM 313
           V +EL  LL+ L G+P GLK+N  L+      F YHI LW +++  + P +  +   +G+
Sbjct: 74  VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGL 133

Query: 314 IGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGR 373
             CLG T  +++  D+I+++  H +CFY+Y   LY ++  GLS LW++ RG ++N+L+ R
Sbjct: 134 SACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQR 193

Query: 374 TESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLP 433
            +S +Y   QL++ T+ F+ ++FLLPTT +YY+VF  L+ L      + H +   +  LP
Sbjct: 194 VDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLP 253

Query: 434 IEVCIKRLLR 443
           +     RL R
Sbjct: 254 LYSLGLRLCR 263
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
           - fission yeast (Schizosaccharomyces pombe)
 emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
           protein gpi1. [Schizosaccharomyces pombe]
          Length = 653

 Score =  327 bits (830), Expect = 4e-88
 Identities = 70/259 (27%), Positives = 122/259 (47%), Gaps = 7/259 (2%)

Query: 185 EYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPAN-IVLDRILGIILMLILFSLATQP 243
           +YM   VF  S  V     + +KE+    +     AN ++    +   ++  L  +    
Sbjct: 291 QYMKLWVFRKSKRVAI---EDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLI 347

Query: 244 GDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPL 303
            +   +   Y I  +  ++  L  +P GLKLN  +  F +    + I++WS FL    P 
Sbjct: 348 ENITFE---YAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPW 404

Query: 304 VRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVR 363
              +   + + G  G +  IAL+ D +SV+ +H H  Y+ +  LYN + R +  L Q+ R
Sbjct: 405 TPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFR 464

Query: 364 GNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFH 423
           G + N+L+ R +S+ Y   QL L TI F+ ++F LPT  V+Y  FA  +      L++  
Sbjct: 465 GKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICE 524

Query: 424 FVRRKLMYLPIEVCIKRLL 442
            +   L + P+ V + R+ 
Sbjct: 525 TMLAFLNHFPLFVTMLRIK 543
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
          Length = 517

 Score =  319 bits (810), Expect = 7e-86
 Identities = 69/259 (26%), Positives = 122/259 (46%), Gaps = 7/259 (2%)

Query: 185 EYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPAN-IVLDRILGIILMLILFSLATQP 243
           +YM   VF  S  V     + +KE+    +     AN ++    +   ++  L  +    
Sbjct: 155 QYMKLWVFRKSKRVAI---EDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHLVVKLI 211

Query: 244 GDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPL 303
            +   +   Y I  +  ++  L  +P GLKLN  +  F +    + I++WS FL    P 
Sbjct: 212 ENITFE---YAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPW 268

Query: 304 VRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVR 363
              +   + + G  G +  IAL+ D +SV+ +H H  Y+ +  +YN + R +  L Q+ R
Sbjct: 269 TPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRMYNWQLRVIYSLLQLFR 328

Query: 364 GNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFH 423
           G + N+L+ R +S+ Y   QL L TI F+ ++F LPT  V+Y  FA  +      L++  
Sbjct: 329 GKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICE 388

Query: 424 FVRRKLMYLPIEVCIKRLL 442
            +   L + P+ V + R+ 
Sbjct: 389 TMLAFLNHFPLFVTMLRIK 407
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
 pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
 emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
          Length = 680

 Score =  303 bits (769), Expect = 4e-81
 Identities = 68/272 (25%), Positives = 121/272 (44%), Gaps = 11/272 (4%)

Query: 178 DSPIKIFEYMAENVFINSI-MVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLIL 236
           +  I+  + +   +F+  I M+  +  KH +E   A  + S  + + +D +LG ++ L L
Sbjct: 290 NFRIRSCQILYWPIFLEEIDMMSISCVKHAEE--AALQRHSTWSAMAVDLVLGNLIGLGL 347

Query: 237 FSLATQPGDFLIQISHYVIDE-LYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWST 295
                    F+   +    +  L      L G P G KLN  L           I++WST
Sbjct: 348 LFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAGVLGMVSLNVIQIWST 407

Query: 296 FLDFIEPLVRQVFLAIGMIGCLGFTF----QIALLVDLISVIGLHSHCFYIYTKVLYNVE 351
              F+      +F  I +I  LG TF      A ++D+I+   LH    +    ++Y+ +
Sbjct: 408 LWVFM---ASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMALHWAITLVYSHQ 464

Query: 352 RRGLSVLWQVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAAL 411
            + L+ LW++ RG + N L+ R +S+ Y  +Q  + ++ F+ +L LLPTT V+YI F   
Sbjct: 465 IQALAALWRLFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTTSVFYIFFTIT 524

Query: 412 KALTFATLSVFHFVRRKLMYLPIEVCIKRLLR 443
                +   +  F    +   P    +  L+R
Sbjct: 525 STTINSICMLIEFAISVIHATPYAEVMIWLVR 556
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
 pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
 emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
          Length = 287

 Score =  253 bits (642), Expect = 4e-66
 Identities = 70/248 (28%), Positives = 106/248 (42%), Gaps = 20/248 (8%)

Query: 196 IMVHTTIYKHFKEWQTACDKRSRPANIVLDRILGIILMLILFSLATQPGDFLIQISHYVI 255
           I  +  + +  +         S    ++LD    I L  I         +     +  V 
Sbjct: 4   ITKNVVLIRTLEAKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVA 63

Query: 256 DELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELW------------------STFL 297
           D L G +  L  +P GLKLN  +N      F YHI LW                  S F+
Sbjct: 64  DNLNGTITWLRSNPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFI 123

Query: 298 DFIEPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSV 357
            F+       F+A  +IG  G +   A++ D   +  LH +CF  Y   L  +    L+V
Sbjct: 124 GFLRSDAFFRFIAYSLIG--GISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTV 181

Query: 358 LWQVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFA 417
           LW +VRG ++N L+ R ++     RQ +LAT  F  +LF+LPT  VY++VF  L+    A
Sbjct: 182 LWSLVRGKKWNPLRERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSA 241

Query: 418 TLSVFHFV 425
             +V +F 
Sbjct: 242 LQTVLYFF 249
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi1p
           [Saccharomyces cerevisiae]
 sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
 gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
          Length = 609

 Score =  245 bits (620), Expect = 1e-63
 Identities = 65/235 (27%), Positives = 119/235 (49%), Gaps = 13/235 (5%)

Query: 217 SRPANIVLDRILGIILMLILFSLATQPGDFLIQISHYVI-----DELYGLLKVLEGSPIG 271
           +    I+ D   G    LIL ++  +  DFL+  SH V+     D L  + + L  +P+G
Sbjct: 280 NTIWLIINDISFG----LILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLG 335

Query: 272 LKLNIHLNNFFLDCFKYHIEL-WSTFLD-FIEP--LVRQVFLAIGMIGCLGFTFQIALLV 327
           +KLN  L NF  + F + IE  ++TF+   I+P  L   + L I M+  +GF+F ++L +
Sbjct: 336 IKLNAELANFLSELFLWVIEFSYTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAI 395

Query: 328 DLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMNRQLYLA 387
           D  +++    + FY  +  LY+ +   ++ L+ +  G + N+L+ R + + +   QL L 
Sbjct: 396 DFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLG 455

Query: 388 TIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRLL 442
           T+ F  ++FL PT + +Y+ +  L+ LT         V   + + P+   + RL 
Sbjct: 456 TLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLK 510
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
          Length = 669

 Score =  234 bits (593), Expect = 2e-60
 Identities = 55/262 (20%), Positives = 113/262 (42%), Gaps = 23/262 (8%)

Query: 201 TIYKHFKEWQTACDKRSRPANIVLDRILGIIL-----------MLILFSLATQPGDFLIQ 249
           +  K+ +        R      +++ I+ I L           ++ L  ++ +   F   
Sbjct: 338 SFMKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIF--- 394

Query: 250 ISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFL 309
              Y    L  +L  L  +P+G KLN +  +F        ++ W  F + I      V  
Sbjct: 395 ---YGTSTLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLN 451

Query: 310 AIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNI 369
            +G    LGF+F ++ ++D +  +  H    Y++ K +  +  + +  L+ +  G ++NI
Sbjct: 452 FVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNI 511

Query: 370 LKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKL 429
           LK R +++ Y N ++ L TI F+ ++FL PT  V  +VF  +  +    + +   + + +
Sbjct: 512 LKLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKII 571

Query: 430 MYLPIEV------CIKRLLRGC 445
           +Y P  +      C K + +G 
Sbjct: 572 LYTPFYIFFIQPNCNKYISKGF 593
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana  (fragment)
 emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
          Length = 143

 Score =  152 bits (382), Expect = 1e-35
 Identities = 36/146 (24%), Positives = 62/146 (41%), Gaps = 8/146 (5%)

Query: 222 IVLDRILGIILMLILFSLATQPGDFLIQISHYVIDE-LYGLLKVLEGSPIGLKLNIHLNN 280
           + +D +LG ++ L L         F+   +    +  L      L G P G KLN  L  
Sbjct: 1   MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 60

Query: 281 FFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTF----QIALLVDLISVIGLH 336
                    I++WST   F+      +F  I +I  LG TF      A ++D+I+   LH
Sbjct: 61  VLGMVSLNVIQIWSTLWVFM---ASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLH 117

Query: 337 SHCFYIYTKVLYNVERRGLSVLWQVV 362
               +    ++Y+ + + L+ LW++ 
Sbjct: 118 IMALHWAITLVYSHQIQALAALWRLF 143
>dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:1333114,
           evidence:ISS~phosphatidylinositol glycan, class
           Q~putative [Mus musculus]
          Length = 378

 Score = 35.4 bits (81), Expect = 1.9
 Identities = 11/59 (18%), Positives = 25/59 (41%), Gaps = 5/59 (8%)

Query: 199 HTTIY---KHFKEWQTACDKRSRPANIVLDRILGIILMLILFSL--ATQPGDFLIQISH 252
           H +     +  +       K +   +++LD  LG++L+  L S     Q  + L+ ++ 
Sbjct: 256 HLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVAD 314
CPU time:   146.30 user secs.	    2.48 sys. secs	  148.78 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.315    0.168    0.502 

Gapped
Lambda     K      H
   0.270   0.0590    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 321734339
Number of Sequences: 887402
Number of extensions: 16633612
Number of successful extensions: 82953
Number of sequences better than 10.0: 162
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 145
Number of HSP's that attempted gapping in prelim test: 82741
Number of HSP's gapped (non-prelim): 304
length of query: 481
length of database: 277,845,442
effective HSP length: 55
effective length of query: 426
effective length of database: 229,038,332
effective search space: 97570329432
effective search space used: 97570329432
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.7 bits)
S2: 75 (33.3 bits)