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GPI Anchor Biosynthesis Report: NP_010725.1 (PIG-P family, Saccharomyces cerevisiae)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (140 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 1

Distribution of 10 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)


Sequences producing significant alignments:


 ref|NP_010725.1|  (NC_001136) Protein required for cell viability...   255  3e-67


Sequences with E-value WORSE than threshold 


 pir||JC7302  Down syndrome critical region protein 5 beta - human       38  0.061

 dbj|BAA95636.1|  (AB035745) DSCR5b [Homo sapiens] >gi|9963906|gb|...    38  0.065

 sp|P57054|DSR5_HUMAN  Down syndrome critical region protein 5 (Do...    38  0.066

 pir||JC7301  Down syndrome critical region protein 5 alpha - huma...    38  0.093

 dbj|BAA95512.1|  (AP001727) Down syndrome critical region protein...    37  0.18

 ref|NP_057514.1|  (NM_016430) Down syndrome critical region prote...    37  0.18

 ref|NP_062416.1|  (NM_019543) Down syndrome critical region prote...    36  0.27

 emb|CAB16583.2|  (Z99295) human downs syndrome critical region-li...    34  1.4

 pir||T38153  hypothetical protein SPAC22A12.13 - fission yeast  (...    33  1.8


Alignments


>ref|NP_010725.1| (NC_001136) Protein required for cell viability; Ydr437wp
           [Saccharomyces cerevisiae]
 pir||S69717 hypothetical protein YDR437w - yeast (Saccharomyces cerevisiae)
 gb|AAB64854.1| (U33007) Ydr437wp; CAI: 0.10 [Saccharomyces cerevisiae]
          Length = 140

 Score =  255 bits (645), Expect = 3e-67
 Identities = 140/140 (100%), Positives = 140/140 (100%)

Query: 1   MYTKEYYWFSQYMIITSTLVLTIIWSILPSSLGEAAPKQFINTLLDIFPQRRWIITLESI 60
           MYTKEYYWFSQYMIITSTLVLTIIWSILPSSLGEAAPKQFINTLLDIFPQRRWIITLESI
Sbjct: 1   MYTKEYYWFSQYMIITSTLVLTIIWSILPSSLGEAAPKQFINTLLDIFPQRRWIITLESI 60

Query: 61  MLMGMLCTYIGLLMYNEDTLTPPLDSLSTVTDAGGQLVIEDDPDVFVKKWAFKETSGIYD 120
           MLMGMLCTYIGLLMYNEDTLTPPLDSLSTVTDAGGQLVIEDDPDVFVKKWAFKETSGIYD
Sbjct: 61  MLMGMLCTYIGLLMYNEDTLTPPLDSLSTVTDAGGQLVIEDDPDVFVKKWAFKETSGIYD 120

Query: 121 LSLMDACQLLYLYDNDHTST 140
           LSLMDACQLLYLYDNDHTST
Sbjct: 121 LSLMDACQLLYLYDNDHTST 140





>pir||JC7302 Down syndrome critical region protein 5 beta - human
          Length = 158

 Score = 38.4 bits (88), Expect = 0.061
 Identities = 31/127 (24%), Positives = 62/127 (48%), Gaps = 17/127 (13%)

Query: 7   YWFSQYMIITSTLVLTIIWSILPSSLGEAAPKQFINTL-LDIFPQRRWIITLESIMLMGM 65
           Y F  ++      +L ++W+ +P S        ++N+L L  +PQ+ W + L   +L+ +
Sbjct: 39  YGFVLFLSSQFGFILYLVWAFIPES--------WLNSLGLTYWPQKYWAVALPVYLLIAI 90

Query: 66  LCTYIGLLMYNEDTLTPPLDSLSTVTDAGGQLVIEDDPDVFVKKWAFKETSGIYDLSLMD 125
           +  Y+ L   N  + T PLDS+ T+TD   +       +   KK+  +    + D+S+ +
Sbjct: 91  VIGYVLLFGLNMMS-TSPLDSIHTITDNYAK-------NQQQKKYQEEAIPALRDISISE 142

Query: 126 ACQLLYL 132
             Q+ +L
Sbjct: 143 VNQMFFL 149





>dbj|BAA95636.1| (AB035745) DSCR5b [Homo sapiens]
 gb|AAG09757.1|AF237812_1 (AF237812) NPD010 [Homo sapiens]
          Length = 158

 Score = 38.4 bits (88), Expect = 0.065
 Identities = 31/127 (24%), Positives = 62/127 (48%), Gaps = 17/127 (13%)

Query: 7   YWFSQYMIITSTLVLTIIWSILPSSLGEAAPKQFINTL-LDIFPQRRWIITLESIMLMGM 65
           Y F  ++      +L ++W+ +P S        ++N+L L  +PQ+ W + L   +L+ +
Sbjct: 39  YGFVLFLSSQFGFILYLVWAFIPES--------WLNSLGLTYWPQKYWAVALPVYLLIAI 90

Query: 66  LCTYIGLLMYNEDTLTPPLDSLSTVTDAGGQLVIEDDPDVFVKKWAFKETSGIYDLSLMD 125
           +  Y+ L   N  + T PLDS+ T+TD   +       +   KK+  +    + D+S+ +
Sbjct: 91  VIGYVLLFGINMMS-TSPLDSIHTITDNYAK-------NQQQKKYQEEAIPALRDISISE 142

Query: 126 ACQLLYL 132
             Q+ +L
Sbjct: 143 VNQMFFL 149





>sp|P57054|DSR5_HUMAN Down syndrome critical region protein 5 (Down syndrome critical
           region protein C)
 dbj|BAA96872.1| (AB037163) DSCR5b [Homo sapiens]
          Length = 158

 Score = 38.4 bits (88), Expect = 0.066
 Identities = 31/127 (24%), Positives = 62/127 (48%), Gaps = 17/127 (13%)

Query: 7   YWFSQYMIITSTLVLTIIWSILPSSLGEAAPKQFINTL-LDIFPQRRWIITLESIMLMGM 65
           Y F  ++      +L ++W+ +P S        ++N+L L  +PQ+ W + L   +L+ +
Sbjct: 39  YGFVLFLSSQFGFILYLVWAFIPES--------WLNSLGLTYWPQKYWAVALPVYLLIAI 90

Query: 66  LCTYIGLLMYNEDTLTPPLDSLSTVTDAGGQLVIEDDPDVFVKKWAFKETSGIYDLSLMD 125
           +  Y+ L   N  + T PLDS+ T+TD   +       +   KK+  +    + D+S+ +
Sbjct: 91  VIGYVLLFGINMMS-TSPLDSIHTITDNYAK-------NQQQKKYQEEAIPALRDISISE 142

Query: 126 ACQLLYL 132
             Q+ +L
Sbjct: 143 VNQMFFL 149





>pir||JC7301 Down syndrome critical region protein 5 alpha - human
 dbj|BAA95633.1| (AB035742) DSCR5a1 [Homo sapiens]
 dbj|BAA95634.1| (AB035743) DSCR5a2 [Homo sapiens]
 dbj|BAA95635.1| (AB035744) DSCR5a3 [Homo sapiens]
 dbj|BAA96871.1| (AB037162) DSCR5a [Homo sapiens]
 dbj|BAB12395.1| (AB039659) PIG-P [Homo sapiens]
 gb|AAH05180.1|AAH05180 (BC005180) Unknown (protein for MGC:12168) [Homo sapiens]
 gb|AAH11007.1|AAH11007 (BC011007) Unknown (protein for MGC:13327) [Homo sapiens]
          Length = 134

 Score = 37.6 bits (86), Expect = 0.093
 Identities = 31/127 (24%), Positives = 62/127 (48%), Gaps = 17/127 (13%)

Query: 7   YWFSQYMIITSTLVLTIIWSILPSSLGEAAPKQFINTL-LDIFPQRRWIITLESIMLMGM 65
           Y F  ++      +L ++W+ +P S        ++N+L L  +PQ+ W + L   +L+ +
Sbjct: 15  YGFVLFLSSQFGFILYLVWAFIPES--------WLNSLGLTYWPQKYWAVALPVYLLIAI 66

Query: 66  LCTYIGLLMYNEDTLTPPLDSLSTVTDAGGQLVIEDDPDVFVKKWAFKETSGIYDLSLMD 125
           +  Y+ L   N  + T PLDS+ T+TD   +       +   KK+  +    + D+S+ +
Sbjct: 67  VIGYVLLFGINMMS-TSPLDSIHTITDNYAK-------NQQQKKYQEEAIPALRDISISE 118

Query: 126 ACQLLYL 132
             Q+ +L
Sbjct: 119 VNQMFFL 125





>dbj|BAA95512.1| (AP001727) Down syndrome critical region protein C [Homo sapiens]
          Length = 108

 Score = 36.8 bits (84), Expect = 0.18
 Identities = 30/115 (26%), Positives = 59/115 (51%), Gaps = 17/115 (14%)

Query: 19  LVLTIIWSILPSSLGEAAPKQFINTL-LDIFPQRRWIITLESIMLMGMLCTYIGLLMYNE 77
           +VL ++W+ +P S        ++N+L L  +PQ+ W + L   +L+ ++  Y+ L   N 
Sbjct: 1   MVLYLVWAFIPES--------WLNSLGLTYWPQKYWAVALPVYLLIAIVIGYVLLFGINM 52

Query: 78  DTLTPPLDSLSTVTDAGGQLVIEDDPDVFVKKWAFKETSGIYDLSLMDACQLLYL 132
            + T PLDS+ T+TD   +       +   KK+  +    + D+S+ +  Q+ +L
Sbjct: 53  MS-TSPLDSIHTITDNYAK-------NQQQKKYQEEAIPALRDISISEVNQMFFL 99





>ref|NP_057514.1| (NM_016430) Down syndrome critical region protein 5; Down syndrome
           critical region protein C short form; Down syndrome
           critical region protein C [Homo sapiens]
 gb|AAF32289.1|AF216305_1 (AF216305) Down syndrome critical region protein C short form [Homo
           sapiens]
 dbj|BAA96873.1| (AB037164) DSCR5c [Homo sapiens]
          Length = 108

 Score = 36.8 bits (84), Expect = 0.18
 Identities = 30/115 (26%), Positives = 59/115 (51%), Gaps = 17/115 (14%)

Query: 19  LVLTIIWSILPSSLGEAAPKQFINTL-LDIFPQRRWIITLESIMLMGMLCTYIGLLMYNE 77
           +VL ++W+ +P S        ++N+L L  +PQ+ W + L   +L+ ++  Y+ L   N 
Sbjct: 1   MVLYLVWAFIPES--------WLNSLGLTYWPQKYWAVALPVYLLIAVVIGYVLLFGINM 52

Query: 78  DTLTPPLDSLSTVTDAGGQLVIEDDPDVFVKKWAFKETSGIYDLSLMDACQLLYL 132
            + T PLDS+ T+TD   +       +   KK+  +    + D+S+ +  Q+ +L
Sbjct: 53  MS-TSPLDSIHTITDNYAK-------NQQQKKYQEEAIPALRDISISEVNQMFFL 99





>ref|NP_062416.1| (NM_019543) Down syndrome critical region protein c [Mus
          musculus]
 gb|AAF32294.1|AF216306_1 (AF216306) DCRC-1 [Mus musculus]
 gb|AAF32295.1|AF216307_1 (AF216307) DCRC-2 [Mus musculus]
 gb|AAF32296.1|AF216308_1 (AF216308) DCRC-3 [Mus musculus]
 gb|AAF32297.1|AF216309_1 (AF216309) DCRC-4 [Mus musculus]
 dbj|BAB23204.1| (AK004169) Down syndrome critical region gene c~data source:MGD,
          source key:MGI:1860433, evidence:ISS~putative [Mus
          musculus]
          Length = 132

 Score = 36.0 bits (82), Expect = 0.27
 Identities = 25/87 (28%), Positives = 46/87 (52%), Gaps = 10/87 (11%)

Query: 7  YWFSQYMIITSTLVLTIIWSILPSSLGEAAPKQFINTL-LDIFPQRRWIITLESIMLMGM 65
          Y F  ++      +L ++W+ +P S        ++N+L L  +PQ+ W + L   +L+ +
Sbjct: 15 YGFVLFLSSQFGFILYLVWAFVPES--------WLNSLGLTYWPQKYWAVALPVYLLITV 66

Query: 66 LCTYIGLLMYNEDTLTPPLDSLSTVTD 92
          +  Y+ L   N  + T PLDS+ T+TD
Sbjct: 67 VIGYVLLFGINMMS-TSPLDSIHTITD 92





>emb|CAB16583.2| (Z99295) human downs syndrome critical region-like
           [Schizosaccharomyces pombe]
          Length = 120

 Score = 33.7 bits (76), Expect = 1.4
 Identities = 24/100 (24%), Positives = 47/100 (47%), Gaps = 8/100 (8%)

Query: 3   TKEYYWFSQYMIITSTLVLTIIWSILPSSLGEAAPKQFINTLLDIFPQRRWIITLESIML 62
           T EYY F  Y++      + I+W++ P+ + +     +       +  R W + + + + 
Sbjct: 6   TYEYYGFVMYLVSMLGFGVYIVWALTPAPVLKFFEIHY-------YLSRWWALAIPTWLF 58

Query: 63  MGMLCTYIGLLMYNEDTLTPPLDSLSTVTDAGGQLVIEDD 102
           + ++  ++ L  YN + LT P  SL  + D    LV E+D
Sbjct: 59  VLVIYIHVVLNAYNTEVLTKPFSSLECIVDQYA-LVGEED 97





>pir||T38153 hypothetical protein SPAC22A12.13 - fission yeast
           (Schizosaccharomyces pombe)
          Length = 157

 Score = 33.3 bits (75), Expect = 1.8
 Identities = 20/90 (22%), Positives = 42/90 (46%), Gaps = 7/90 (7%)

Query: 3   TKEYYWFSQYMIITSTLVLTIIWSILPSSLGEAAPKQFINTLLDIFPQRRWIITLESIML 62
           T EYY F  Y++      + I+W++ P+ + +     +       +  R W + + + + 
Sbjct: 43  TYEYYGFVMYLVSMLGFGVYIVWALTPAPVLKFFEIHY-------YLSRWWALAIPTWLF 95

Query: 63  MGMLCTYIGLLMYNEDTLTPPLDSLSTVTD 92
           + ++  ++ L  YN + LT P  SL  + D
Sbjct: 96  VLVIYIHVVLNAYNTEVLTKPFSSLECIVD 125





CPU time:    37.17 user secs.	    1.68 sys. secs	   38.85 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.323    0.139    0.426 

Gapped
Lambda     K      H
   0.270   0.0487    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85126555
Number of Sequences: 887402
Number of extensions: 3218562
Number of successful extensions: 7551
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 7541
Number of HSP's gapped (non-prelim): 13
length of query: 140
length of database: 277,845,442
effective HSP length: 51
effective length of query: 89
effective length of database: 232,587,940
effective search space: 20700326660
effective search space used: 20700326660
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 69 (31.2 bits)