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GPI Anchor Biosynthesis Report: T02245 (PIG-O family, Homo sapiens)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (880 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Results of PSI-Blast iteration 4

Distribution of 58 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)
Sequences producing significant alignments: (bits) Value
pir||T02245 hypothetical protein P1.11659_3 - human >gi|2984587|... 1263 0.0
ref|NP_064419.1| (NM_020035) phosphatidylinositol glycan, class ... 529 e-149
gb|AAF59237.1| (AE003840) CG2144 gene product [Drosophila melano... 450 e-125
gb|AAF57689.1| (AE003799) CG12263 gene product [Drosophila melan... 443 e-123
ref|NP_197227.1| (NM_121731) unknown protein [Arabidopsis thalia... 422 e-116
gb|AAK52677.1|AF321466_1 (AF321466) putative protein Gpi7p [Yarr... 412 e-113
ref|NP_013069.1| (NC_001144) Glycosylphosphatidylinositol (GPI) ... 407 e-112
dbj|BAA82663.1| (AB030316) phosphatidylinositolglycan class N [M... 393 e-108
dbj|BAA82620.1| (AB030280) phosphatidylinositolglycan class N sh... 387 e-106
ref|NP_038812.1| (NM_013784) phosphatidylinositol glycan, class ... 385 e-105
gb|AAH21148.1|AAH21148 (BC021148) Similar to phosphatidylinosito... 384 e-105
ref|NP_036459.1| (NM_012327) phosphatidylinositol glycan, class ... 375 e-102
ref|NP_012756.1| (NC_001143) Required for GPI anchor synthesis; ... 374 e-102
ref|NP_012473.1| (NC_001142) Local Anesthetics Sensitive: involv... 360 6e-98
gb|AAF59557.2| (AC024812) Hypothetical protein Y54E10BR.1 [Caeno... 352 1e-95
ref|NP_491097.1| (NM_058696) Y54E10BR.1.p [Caenorhabditis elegans] 352 1e-95
ref|NP_491750.1| (NM_059349) C27A12.9.p [Caenorhabditis elegans]... 343 6e-93
gb|AAF58853.1| (AE003831) CG2292 gene product [Drosophila melano... 339 9e-92
pir||T40715 hypothetical protein SPBC839.08c - fission yeast (S... 334 4e-90
dbj|BAB29186.1| (AK014165) data source:MGD, source key:MGI:13516... 333 7e-90
ref|NP_186787.1| (NM_111004) putative phosphatidylinositolglycan... 328 2e-88
sp|Q09782|YA93_SCHPO HYPOTHETICAL 85.7 KD PROTEIN C13G6.03 IN CH... 323 6e-87
ref|XP_056208.1| (XM_056208) hypothetical protein MGC3079 [Homo ... 304 4e-81
ref|NP_116023.1| (NM_032634) hypothetical protein MGC3079 [Homo ... 298 3e-79
gb|AAF56048.1| (AE003740) CG4907 gene product [Drosophila melano... 285 2e-75
pir||T46311 hypothetical protein DKFZp434H0711.1 - human (fragme... 252 1e-65
ref|NP_281901.1| (NC_002163) hypothetical protein Cj0729 [Campyl... 236 1e-60
dbj|BAB17014.1| (AB050262) hypothetical protein [Macaca fascicul... 206 1e-51
gb|AAF56711.1| (AE003761) CG13978 gene product [Drosophila melan... 196 1e-48
ref|NP_251941.1| (NC_002516) hypothetical protein [Pseudomonas a... 191 4e-47
pir||T33724 hypothetical protein - Zymomonas mobilis >gi|3089614... 188 4e-46
dbj|BAA87261.1| (AB027957) Hypothetical protein [Schizosaccharom... 177 6e-43
dbj|BAB29052.1| (AK013913) data source:MGD, source key:MGI:18614... 175 3e-42
ref|NP_391271.1| (NC_000964) phosphoglycerate mutase [Bacillus s... 153 9e-36
pir||T46865 phosphoglycerate mutase (EC 5.4.2.1), 2, 3-diphospho... 153 1e-35
pdb|1EJJ|A Chain A, Crystal Structural Analysis Of Phosphoglycer... 153 1e-35
ref|NP_347349.1| (NC_003030) 2,3-bisphosphoglycerate-independent... 150 7e-35
pir||I40024 phosphoglycerate mutase - Bacillus subtilis >gi|4602... 150 1e-34
ref|NP_471880.1| (NC_003212) highly similar to phosphoglycerate ... 147 7e-34
ref|NP_465979.1| (NC_003210) highly similar to phosphoglycerate ... 147 8e-34
ref|NP_371299.1| (NC_002758) 2, 3-diphosphoglycerate-independent... 147 9e-34
ref|NP_562217.1| (NC_003366) 2,3-bisphosphoglycerate-independent... 146 1e-33
gb|AAF54657.1| (AE003692) CG6790 gene product [Drosophila melano... 142 2e-32
ref|NP_244424.1| (NC_002570) 2,3-bisphosphoglycerate-independent... 139 1e-31
sp|P52832|PMGI_PSESM 2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHO... 138 4e-31
gb|AAD26327.1|AF120090_1 (AF120090) 2,3-bisphosphoglycerate-inde... 128 3e-28
Alignments
>pir||T02245 hypothetical protein P1.11659_3 - human
 gb|AAC07985.1| (AC004472) P1.11659_3 [Homo sapiens]
          Length = 880

 Score = 1263 bits (3233), Expect = 0.0
 Identities = 880/880 (100%), Positives = 880/880 (100%)

Query: 1   MQKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGA 60
           MQKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGA
Sbjct: 1   MQKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGA 60

Query: 61  CWMASRFSRVVLVLIDALRFDFAQPQHSHRILEIQPHHARLYRSQVDPPTTTMQRLKALT 120
           CWMASRFSRVVLVLIDALRFDFAQPQHSHRILEIQPHHARLYRSQVDPPTTTMQRLKALT
Sbjct: 61  CWMASRFSRVVLVLIDALRFDFAQPQHSHRILEIQPHHARLYRSQVDPPTTTMQRLKALT 120

Query: 121 TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSF 180
           TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSF
Sbjct: 121 TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSF 180

Query: 181 NVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVI 240
           NVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVI
Sbjct: 181 NVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVI 240

Query: 241 QGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIP 300
           QGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIP
Sbjct: 241 QGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIP 300

Query: 301 QVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLH 360
           QVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLH
Sbjct: 301 QVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLH 360

Query: 361 TYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMC 420
           TYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMC
Sbjct: 361 TYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMC 420

Query: 421 IESWARFSLVRMAGAKNLWYGACVAALVALLAAVRLWLRRYGNLKSPEPPMLFVRWGLPL 480
           IESWARFSLVRMAGAKNLWYGACVAALVALLAAVRLWLRRYGNLKSPEPPMLFVRWGLPL
Sbjct: 421 IESWARFSLVRMAGAKNLWYGACVAALVALLAAVRLWLRRYGNLKSPEPPMLFVRWGLPL 480

Query: 481 MALGTAAYWALASGADEAPPRLRVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLVKAG 540
           MALGTAAYWALASGADEAPPRLRVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLVKAG
Sbjct: 481 MALGTAAYWALASGADEAPPRLRVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLVKAG 540

Query: 541 AGAPRTRTVLTPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQLGS 600
           AGAPRTRTVLTPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQLGS
Sbjct: 541 AGAPRTRTVLTPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQLGS 600

Query: 601 VYSAAMVTALTLLAFPLLLLHAERISLVFLLLFLQSFLLLHLLAAGIPVTTPGKYLSSDS 660
           VYSAAMVTALTLLAFPLLLLHAERISLVFLLLFLQSFLLLHLLAAGIPVTTPGKYLSSDS
Sbjct: 601 VYSAAMVTALTLLAFPLLLLHAERISLVFLLLFLQSFLLLHLLAAGIPVTTPGKYLSSDS 660

Query: 661 LKDNSDVSSAPLVFKEVLLLMFLSLTEGPMPHTTRKVFLVSSLLPAIAKQIDPSCWFPGF 720
           LKDNSDVSSAPLVFKEVLLLMFLSLTEGPMPHTTRKVFLVSSLLPAIAKQIDPSCWFPGF
Sbjct: 661 LKDNSDVSSAPLVFKEVLLLMFLSLTEGPMPHTTRKVFLVSSLLPAIAKQIDPSCWFPGF 720

Query: 721 MERRDKESSKTPCPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGS 780
           MERRDKESSKTPCPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGS
Sbjct: 721 MERRDKESSKTPCPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGS 780

Query: 781 CTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEE 840
           CTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEE
Sbjct: 781 CTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEE 840

Query: 841 EPLMEMRLRDAPQHFYAALLQLGLKYLFILGSYLRLWASL 880
           EPLMEMRLRDAPQHFYAALLQLGLKYLFILGSYLRLWASL
Sbjct: 841 EPLMEMRLRDAPQHFYAALLQLGLKYLFILGSYLRLWASL 880
>ref|NP_064419.1| (NM_020035) phosphatidylinositol glycan, class O [Mus musculus]
 dbj|BAA96254.1| (AB038560) Pig-o [Mus musculus]
          Length = 1101

 Score =  529 bits (1350), Expect = e-149
 Identities = 387/452 (85%), Positives = 401/452 (88%), Gaps = 20/452 (4%)

Query: 1   MQKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGA 60
           M+K SVLLFLAWVCFLFYAGIALFTSGFLLTRLELTN SSCQE PGPG LPWGS GKPGA
Sbjct: 9   MRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGA 68

Query: 61  CWMASRFSRVVLVLIDALRFDFAQPQHSH-------------------RILEIQPHHARL 101
           CWM SRFSRVVLVLIDALRFDFAQPQ SH                   RILE QPHH RL
Sbjct: 69  CWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRL 128

Query: 102 YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDD 161
           YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDN+I+QL SAGRRVVFMGDD
Sbjct: 129 YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDD 188

Query: 162 TWKDLFPGAFSKAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHK 221
           TW+DLFPGAFS+AFFF SFNVRDL TVDNGILEHLYPT+D G WDVLIAHFLGVDHCGHK
Sbjct: 189 TWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHK 248

Query: 222 HGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFL 281
           HGPHHPEMAKKLSQMDQVIQGL+ERLENDTLLVVAGDHGMT NGDHGGDSELEVSAALFL
Sbjct: 249 HGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSAALFL 308

Query: 282 YSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGED-SQPHSS 340
           YSPTA+FPS PPEEPEVIPQVSLVPTLALLLGLPIPFGN GEVMAELFSGG D S PHSS
Sbjct: 309 YSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSS 368

Query: 341 ALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGAEA 400
           ALAQ SALH+NAQQVSRFLHTYSAATQDLQ KELH+LQ LFSKASA YQ  L+ P+ AEA
Sbjct: 369 ALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEA 428

Query: 401 TLPTVIAELQQFLRGARAMCIESWARFSLVRM 432
            L T+ AE QQFLRGARA+CIESWARFSLVRM
Sbjct: 429 ALSTLTAEFQQFLRGARALCIESWARFSLVRM 460
 Score =  271 bits (688), Expect = 3e-71
 Identities = 196/238 (82%), Positives = 208/238 (87%), Gaps = 2/238 (0%)

Query: 420 CIESWARFSLVRMAGA--KNLWYGACVAALVALLAAVRLWLRRYGNLKSPEPPMLFVRWG 477
           C  S     L  M G   KNLWYGACV ALVALL  VRLWLRRYGNLKSPEPP+LFVRWG
Sbjct: 657 CRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWG 716

Query: 478 LPLMALGTAAYWALASGADEAPPRLRVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLV 537
           +PLM LGTAAYWALASGA+EAPPRLR LV+GAS VLPRAV GLAA GL LLLW+PVTVLV
Sbjct: 717 MPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLV 776

Query: 538 KAGAGAPRTRTVLTPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQ 597
           KAGA   RTRT+LTPFSGPPTSQADLDYVVPQIYRHMQEEF+GRLERTK+QGP+TVAAYQ
Sbjct: 777 KAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQ 836

Query: 598 LGSVYSAAMVTALTLLAFPLLLLHAERISLVFLLLFLQSFLLLHLLAAGIPVTTPGKY 655
           LGSVYSAAMVTAL LLAFPL+LLH ER+SLVFLLLFLQSFLLLHLLAAG PV TPG +
Sbjct: 837 LGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPF 894
 Score =  256 bits (649), Expect = 9e-67
 Identities = 119/147 (80%), Positives = 130/147 (87%), Gaps = 3/147 (2%)

Query: 731  TPCPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVG 790
            TP PFTV WQAVSAW L+ATQTFYSTGHQPVF AIHWHAAFVGFP+GHGS TWLPALLVG
Sbjct: 890  TPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVG 949

Query: 791  ANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVR---PEEEEEPLMEMR 847
            ANTFASHLLFAVGCPLLLLWPFLCE QG ++RQ  PG+E++ARVR    EE +EPLME+R
Sbjct: 950  ANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEVR 1009

Query: 848  LRDAPQHFYAALLQLGLKYLFILGSYL 874
            LRDAP HF AALLQLGLKYLFILG+ +
Sbjct: 1010 LRDAPNHFNAALLQLGLKYLFILGAQI 1036
>gb|AAF59237.1| (AE003840) CG2144 gene product [Drosophila melanogaster]
          Length = 880

 Score =  450 bits (1146), Expect = e-125
 Identities = 136/517 (26%), Positives = 215/517 (41%), Gaps = 50/517 (9%)

Query: 2   QKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGAC 61
           Q  S L++   +   F  G  LF  GF      +    S   P G  +   G +  P   
Sbjct: 3   QHKSWLVYTIGMLTAFLCGAVLFLIGFFPASYSVAEKESTV-PEGRPTALLGMELTPP-- 59

Query: 62  WMASRFSRVVLVLIDALRFDF----AQPQHSHRILEIQPHHARLYRSQVDPPTTTMQRLK 117
                +   VL+L+DALR DF    + P    R  E         +  VD PT TM RLK
Sbjct: 60  --PPAYDSFVLLLVDALRDDFPDATSMPVAYSRACEKL-------KLHVDIPTVTMPRLK 110

Query: 118 ALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKA-FF 176
           ++TTG+L  FID   N      ++D+ + +L    R V F GD TW  LFP  F++    
Sbjct: 111 SITTGTLSNFIDIALNVGHTEQMQDSFLHRLKQQNRVVSFAGDHTWVKLFPSEFTRQVEN 170

Query: 177 FPSFNVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQM 236
             SF V D    D  + + L   ++  +W +LI H+LG+DH GH  G   P +  KL +M
Sbjct: 171 HDSFYVNDFYEGDRNVTKTLETELERSDWSLLILHYLGLDHIGHVEGNASPRVPLKLKEM 230

Query: 237 DQVIQGLVERL-ENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEE 295
           D+V++ +++     + LL++ GDHGM   G HGG++  E    L+LY          P  
Sbjct: 231 DEVVKKILDHKSFPNVLLMLTGDHGMADGGGHGGNTPAETLVPLYLYYNNCS---KTPSA 287

Query: 296 PEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNA--- 352
            +   Q+ L PTL++LL + IP  +IG ++ E+           +    A  L   A   
Sbjct: 288 SKRYNQIDLAPTLSVLLSVEIPTLSIGCLIPEMLQSLSLEHQLYAYFYNAHHLLNKARVK 347

Query: 353 -----QQVSRFLHTYSAAT-------------QDLQAKELHQLQNLFSKASADYQWLLQS 394
                   S +   Y  AT                 A +++     +  A  +Y  L   
Sbjct: 348 FGHEIVHRSDYYTWYQNATLLHKKLLRPLREGDGGGASQVY-----YQNAKLNYMRLQLD 402

Query: 395 PKGAEATLPTVI-AELQQFLRGARAMCIESWARFSLVRMAGAKNLWYG-ACVAALVALLA 452
                    +VI A +  +   A  + + S   +S++ +       Y    VA  +  +A
Sbjct: 403 GLKVGQLWLSVIAAVVLNYTSHALDIIMTSSTLYSILLLVPISIAVYLTIDVAQALLKIA 462

Query: 453 AVRLWLRRYGNLKSPEPPMLFVRWGLPLMALGTAAYW 489
              L+ RR   +  P P  L   + +  + LG++++ 
Sbjct: 463 LPPLYTRRL-KISIPLPLPLLACYAIRTLTLGSSSFI 498
>gb|AAF57689.1| (AE003799) CG12263 gene product [Drosophila melanogaster]
          Length = 1077

 Score =  443 bits (1129), Expect = e-123
 Identities = 180/461 (39%), Positives = 260/461 (56%), Gaps = 40/461 (8%)

Query: 5   SVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGS------------LPW 52
           + L  L W+ FL  +G+ LF+ GFLL R+  T  S+C+      +            +  
Sbjct: 4   TYLFVLIWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEIFK 63

Query: 53  GSQGKPGACWMASRFSRVVLVLIDALRFDFAQPQHS--------------HRILEIQPHH 98
                   C    + S+V+++++DAL+++F   + +                +L+  P H
Sbjct: 64  DVNASSNLCL--PQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDH 121

Query: 99  ARLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFM 158
           ARL R + DPPTTT+QRLK LTTGSLPTFID GSNFAS  I EDN+I Q+      VVF+
Sbjct: 122 ARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVFL 181

Query: 159 GDDTWKDLFPGAFSKAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHC 218
           GD TW DL+P  F +++ +PSF++ DLD+VDN IL+HL   ++S +W VL+AHFLGVDHC
Sbjct: 182 GDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDHC 241

Query: 219 GHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAA 278
           GHKHGP H EMA+KL +M++VI+ +V  ++NDT L+V GDHGMT +GDHGGD++ E +A 
Sbjct: 242 GHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDHGMTASGDHGGDTDDETNAL 301

Query: 279 LFLYSPTAVFPSTP-PEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQP 337
           LF YS    F       + E++ Q+ LVPTLA +LG+PIP+ N+G V   +        P
Sbjct: 302 LFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLR--VP 359

Query: 338 HSSALAQASALH--LNAQQVSRFLHTYSAA-TQDLQAKELHQLQNLFSKASADYQWLLQS 394
           H +   Q   LH   NAQQ+ R+   Y+    +    +++  L+  F   +   Q +   
Sbjct: 360 HLNKF-QTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQTVYN- 417

Query: 395 PKGAEATLPTVIAELQQFLRGARAMCIESWARFSLVRMAGA 435
               E    + + +L   LR     C E W RF   +M+  
Sbjct: 418 ----EVAFKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQG 454
 Score =  229 bits (578), Expect = 2e-58
 Identities = 52/203 (25%), Positives = 87/203 (42%), Gaps = 23/203 (11%)

Query: 425 ARFSLVRMAGAKNLWYGACVAALVALLAAVRLWLRRYGNLKSPEPPMLFVRWGLPLMALG 484
           A FSL +    K   Y   V  LV      RL+LR  GNL    P +L  R+G  + ++ 
Sbjct: 655 AGFSLKKPGTGKT--YILAVVVLVVYTTLTRLYLRSCGNLTGNLPNVLLARYGPTVASIC 712

Query: 485 TAAYWALASGADEAPPRLRVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLVKAGAGAP 544
              +  LA+ + +   R  +     +M L   + GL    + +L W P+           
Sbjct: 713 AGGHILLANSSIKHIQRTHI----DAMAL--VIYGLLLVQIIVLSWAPLMTF-------- 758

Query: 545 RTRTVLTPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQLGSVYSA 604
               VL P S    +    + +VP+I+R M+  + G  +  +S  P+    Y L +VYS+
Sbjct: 759 ----VLPPRSSHTVTINGNESIVPEIFRKMKRMYEGDDDERRSHIPVV---YGLATVYSS 811

Query: 605 AMVTALTLLAFPLLLLHAERISL 627
            ++     LA  +++L   R S+
Sbjct: 812 IVIAFGVFLALVMIVLLEPRASI 834
 Score =  109 bits (270), Expect = 2e-22
 Identities = 23/60 (38%), Positives = 29/60 (48%)

Query: 741 AVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVGANTFASHLLF 800
           A+  W L+A   F++T HQ     I W AAFVG   G G    +   LV  NTF   + F
Sbjct: 871 ALVGWFLLAHFCFFATSHQTTLSQIEWRAAFVGRTTGIGQSNLVSGALVILNTFCGPIFF 930
>ref|NP_197227.1| (NM_121731) unknown protein [Arabidopsis thaliana]
 dbj|BAB10512.1| (AB005238) gene_id:MKP11.10~unknown protein [Arabidopsis thaliana]
          Length = 884

 Score =  422 bits (1074), Expect = e-116
 Identities = 175/502 (34%), Positives = 242/502 (47%), Gaps = 81/502 (16%)

Query: 8   LFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLP-------WGSQGKPGA 60
           L       +    I +FT GFLLTR EL  HS+C +      L          S+ KP  
Sbjct: 10  LTALGFLLIHAIAILIFTRGFLLTRTELPFHSTCSDVSLSPCLASPRSNHDSSSEPKP-- 67

Query: 61  CWMASRFSRVVLVLIDALRFDFAQPQHSHRILEIQPHHARLYRSQVDPPTTTMQRLKALT 120
            WM        L ++  L F                  A+++++  DPPTT++QRLK LT
Sbjct: 68  -WMDK------LTILQTLAFA-------------NDSSAKIFKAFADPPTTSLQRLKGLT 107

Query: 121 TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSF 180
           TG LPTFID G++F + AIVEDN I QL   G+R+V MGDDTW  LFP  F K++ FPSF
Sbjct: 108 TGGLPTFIDIGNSFGAPAIVEDNFINQLVLNGKRLVMMGDDTWTQLFPNQFQKSYPFPSF 167

Query: 181 NVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVI 240
           NV+DLDTVDNG +EHL+PT+   +WDVLIAHFLGVDH GH  G     M  KL Q + V+
Sbjct: 168 NVKDLDTVDNGCIEHLFPTLFKDDWDVLIAHFLGVDHAGHIFGVDSSPMINKLEQYNSVL 227

Query: 241 QGLVERLEN---------DTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVFPST 291
           + ++  LE+         +T+L+V GDHG T NGDHGG +  EV   +F  S        
Sbjct: 228 EKVINILESQAGPGGLHENTMLIVMGDHGQTLNGDHGGGTAEEVETTMFAMSTKKHTTLV 287

Query: 292 PPE-EPEVIPQ--------VSLVPTLALLLGLPIPFGN----IGEVMAELFSGGE----- 333
           PPE +     Q        +S +      L L  PF +    IG V  EL++ G      
Sbjct: 288 PPEFDTSSCKQNKDGKQMCISYIEQQHCQLCLGYPFLSEGAFIGHVNPELYALGSSSWNL 347

Query: 334 DSQPHSSALAQASA----------LHLNAQQVSRFLHTYSA-ATQDLQAKELHQLQNLFS 382
           D     +   Q++A          L +NA QV R++  YS  +     + ++ ++ +L+S
Sbjct: 348 DKSDLGNFGTQSAANEWMKHYVDVLCVNAWQVKRYIDVYSNSSVVGFSSDDMSRISDLYS 407

Query: 383 KASADY----QWLLQSPKGAE------ATLPTVIAELQQFLRGARAMCIESWARFSLVRM 432
            A  ++    + +L    G +      A L   IAE   F      +    W  F+L  M
Sbjct: 408 AAEQNWSNSVKHILMDRNGDDGSTDISALLKEQIAEYLNFFSSVVELARSKWTEFNLNLM 467

Query: 433 AGAKNLWYGACVAALVALLAAV 454
                  +G  V +L+    AV
Sbjct: 468 ITG----FGILVISLILQFLAV 485
 Score =  422 bits (1074), Expect = e-116
 Identities = 175/502 (34%), Positives = 242/502 (47%), Gaps = 81/502 (16%)

Query: 8   LFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLP-------WGSQGKPGA 60
           L       +    I +FT GFLLTR EL  HS+C +      L          S+ KP  
Sbjct: 10  LTALGFLLIHAIAILIFTRGFLLTRTELPFHSTCSDVSLSPCLASPRSNHDSSSEPKP-- 67

Query: 61  CWMASRFSRVVLVLIDALRFDFAQPQHSHRILEIQPHHARLYRSQVDPPTTTMQRLKALT 120
            WM        L ++  L F                  A+++++  DPPTT++QRLK LT
Sbjct: 68  -WMDK------LTILQTLAFA-------------NDSSAKIFKAFADPPTTSLQRLKGLT 107

Query: 121 TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSF 180
           TG LPTFID G++F + AIVEDN I QL   G+R+V MGDDTW  LFP  F K++ FPSF
Sbjct: 108 TGGLPTFIDIGNSFGAPAIVEDNFINQLVLNGKRLVMMGDDTWTQLFPNQFQKSYPFPSF 167

Query: 181 NVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVI 240
           NV+DLDTVDNG +EHL+PT+   +WDVLIAHFLGVDH GH  G     M  KL Q + V+
Sbjct: 168 NVKDLDTVDNGCIEHLFPTLFKDDWDVLIAHFLGVDHAGHIFGVDSSPMINKLEQYNSVL 227

Query: 241 QGLVERLEN---------DTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVFPST 291
           + ++  LE+         +T+L+V GDHG T NGDHGG +  EV   +F  S        
Sbjct: 228 EKVINILESQAGPGGLHENTMLIVMGDHGQTLNGDHGGGTAEEVETTMFAMSTKKHTTLV 287

Query: 292 PPE-EPEVIPQ--------VSLVPTLALLLGLPIPFGN----IGEVMAELFSGGE----- 333
           PPE +     Q        +S +      L L  PF +    IG V  EL++ G      
Sbjct: 288 PPEFDTSSCKQNKDGKQMCISYIEQQHCQLCLGYPFLSEGAFIGHVNPELYALGSSSWNL 347

Query: 334 DSQPHSSALAQASA----------LHLNAQQVSRFLHTYSA-ATQDLQAKELHQLQNLFS 382
           D     +   Q++A          L +NA QV R++  YS  +     + ++ ++ +L+S
Sbjct: 348 DKSDLGNFGTQSAANEWMKHYVDVLCVNAWQVKRYIDVYSNSSVVGFSSDDMSRISDLYS 407

Query: 383 KASADY----QWLLQSPKGAE------ATLPTVIAELQQFLRGARAMCIESWARFSLVRM 432
            A  ++    + +L    G +      A L   IAE   F      +    W  F+L  M
Sbjct: 408 AAEQNWSNSVKHILMDRNGDDGSTDISALLKEQIAEYLNFFSSVVELARSKWTEFNLNLM 467

Query: 433 AGAKNLWYGACVAALVALLAAV 454
                  +G  V +L+    AV
Sbjct: 468 ITG----FGILVISLILQFLAV 485
 Score = 40.1 bits (93), Expect = 0.17
 Identities = 32/107 (29%), Positives = 53/107 (48%), Gaps = 10/107 (9%)

Query: 721 MERRDKESSKTPCPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGS 780
           +ERR K   ++      P   V  W+L++   F+++GH   F  + + AAFVGF E    
Sbjct: 722 VERRTKSDGQSKFSKLNPL-CVVQWSLLSICMFFASGHWCAFDGLRYGAAFVGFDEFV-- 778

Query: 781 CTWLPALLVGANTFASHLLFAV-GCPLLLLWPFLCES---QGLRKRQ 823
                A+L+   TF   ++ +V G PLL+  PF  ++    G ++ Q
Sbjct: 779 -LIRQAILLTIETFGFSIILSVFGLPLLI--PFHSQTPQAHGEKRHQ 822
>gb|AAK52677.1|AF321466_1 (AF321466) putative protein Gpi7p [Yarrowia lipolytica]
          Length = 860

 Score =  412 bits (1049), Expect = e-113
 Identities = 135/482 (28%), Positives = 203/482 (42%), Gaps = 60/482 (12%)

Query: 1   MQKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGA 60
           M      L    +  L  A + LF  GFL +R+ L  ++  +                  
Sbjct: 1   MLWKRWTL-AVTIVVLQLAAVLLFARGFLPSRVLLPGYTESRVSTEA------------- 46

Query: 61  CWMASRFSRVVLVLIDALRFDFAQPQHSH---RILEIQPHHARLYRSQVDPPTTTMQRLK 117
                 F + +++++DA R DFA    S+       I    A  + +   PPT T+ R+K
Sbjct: 47  -----PFQKAIIMVVDAFRSDFAFSDQSNCPQLHKRINSGGAIPFTAHSTPPTVTLPRIK 101

Query: 118 ALTTGSLPTFIDAGSNFASHA-----IVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFS 172
            LTTGS P F+DA  N A          +D+ + Q +  GR++   GDDTW  LFPG F 
Sbjct: 102 GLTTGSTPNFLDAVLNIAESDNSSTLANQDSWLAQASRDGRKIHMFGDDTWIKLFPGMFD 161

Query: 173 KAFFFPSFNVRDLDTVDNGILEHLYPTMDSG-EWDVLIAHFLGVDHCGHKHGPHHPEMAK 231
                 SF V D   VDN +  H+   +D   EWDVLI H+LG+DH GHK GP  P M  
Sbjct: 162 DCEGTASFFVSDYTEVDNNVTRHIDTQLDQKTEWDVLILHYLGLDHIGHKTGPESPFMPA 221

Query: 232 KLSQMDQVIQGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVF--- 288
           K  +MD +   L    ++DT+L++ GDHGM   G+HGG S  E SAA+   SP       
Sbjct: 222 KQKEMDDIFDKLYNSCDDDTVLILLGDHGMNEVGNHGGSSAGETSAAMVFASPKFETAQL 281

Query: 289 ---------PSTPPEEPE-VIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPH 338
                    P T   +    + Q  LVPTL  LLGL  P  N+G +++++          
Sbjct: 282 TETAETSPLPWTDTYKYHSRMDQTDLVPTLTALLGLNTPKNNLGVLVSQMLGLWS----- 336

Query: 339 SSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGA 398
                Q + L  NA Q+ + L     A+++  AKE+++L +    ++   +        A
Sbjct: 337 --PEDQLNVLKNNADQMVQILQ--GQASRESDAKEVYELYDTL-NSNPSVKDYYNFLYEA 391

Query: 399 EATLPTVIAELQQF------LRGARAMCIESWARFSLVRMAGAKNLWYGACVAALVALLA 452
           ++ L    +             G  +  +      +L          Y      L+    
Sbjct: 392 QSYLTHASSNYNTNDMLGGIGLGLLSTILALTVFSALTLAVQGLKRLYLII---LLVYFI 448

Query: 453 AV 454
           +V
Sbjct: 449 SV 450
>ref|NP_013069.1| (NC_001144) Glycosylphosphatidylinositol (GPI) biosynthesis; Gpi13p
           [Saccharomyces cerevisiae]
 pir||S64782 probable membrane protein YLL031c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97480.1| (Z73136) ORF YLL031c [Saccharomyces cerevisiae]
          Length = 1017

 Score =  407 bits (1036), Expect = e-112
 Identities = 172/489 (35%), Positives = 236/489 (48%), Gaps = 84/489 (17%)

Query: 3   KASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACW 62
               ++ L ++  L +  IA FT GFLL+R  L N SS  E                   
Sbjct: 32  HKFYIILLVFIAILQFISIAFFTRGFLLSRHVLDNISSQNETS----------------K 75

Query: 63  MASRFSRVVLVLIDALRFDFAQP---QHSHRILEIQPHHARLYRSQ-------------- 105
           +  RF++ V+++IDALRFDFA P    HS+  L    +   LY S               
Sbjct: 76  LPPRFNKAVILVIDALRFDFAIPVNESHSNYNLNYHNNILSLYDSFASDKDASSLLLKFI 135

Query: 106 VDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKD 165
            DPPTTT+QRLK LTTGSLPTFIDAGSNF    I EDN +KQL  A + V F GDDTW  
Sbjct: 136 ADPPTTTLQRLKGLTTGSLPTFIDAGSNFDGTVIEEDNFLKQLHLANKTVKFAGDDTWMA 195

Query: 166 LF-PGAFSKAFFFPSFNVRDLDTVDNGILEHLYPTMDSGE-WDVLIAHFLGVDHCGHKHG 223
           LF P   + +F   S NV DLDTVDNG++++ +  +   + WDV+I H LG+DH GHK+G
Sbjct: 196 LFHPFLSNDSFPLESLNVWDLDTVDNGVMDYFHDHLQQDKEWDVMIGHMLGIDHVGHKYG 255

Query: 224 PHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYS 283
           P H  M +K  Q+DQ I  +++ +++DTLLV+ GDHGM   G+HGGDS  E+ + LFLYS
Sbjct: 256 PDHFTMREKQIQVDQFIDWILKSIDDDTLLVILGDHGMDHTGNHGGDSIDELESTLFLYS 315

Query: 284 PTAV----------FPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGE 333
                                +   + Q+ LV +LALL+G PIPF N+G  + E+     
Sbjct: 316 KKPDMWRLKETSNYNIDNLGHDYRSVRQIDLVSSLALLMGQPIPFNNLGWPIDEIARNDR 375

Query: 334 D-SQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQL-----QNLFSKASAD 387
           + SQ  +SA++Q              +H  +    +  AK +           F K    
Sbjct: 376 EWSQFVNSAISQLQLYKDTM-----QIHHGNDEILEPLAKNISNTPPTSDPEKFVKLGHK 430

Query: 388 YQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWARFSLVRMAGAKNLWYGACVAAL 447
           YQ +                            C E WA+F    +A    L       +L
Sbjct: 431 YQKVF------------------------LQTCEELWAKFDYYSIATGITL----LATSL 462

Query: 448 VALLAAVRL 456
           V L++  +L
Sbjct: 463 VLLISITKL 471
 Score = 45.2 bits (106), Expect = 0.004
 Identities = 22/69 (31%), Positives = 33/69 (46%), Gaps = 13/69 (18%)

Query: 747 LMATQTFYSTGHQPVFPAIHWHAAF-----VGFPEGHGSCTWLPALLVGANTFASHLLFA 801
           L++ Q F++TGHQ   P++ W   F     V FP           + +  NTF  H+L +
Sbjct: 866 LLSYQHFFTTGHQATIPSVQWDIGFMLSEKVTFP--------FTQIAIILNTFGPHILVS 917

Query: 802 VGCPLLLLW 810
           +   LL LW
Sbjct: 918 LSVALLTLW 926
>dbj|BAA82663.1| (AB030316) phosphatidylinositolglycan class N [Mus musculus]
          Length = 391

 Score =  393 bits (999), Expect = e-108
 Identities = 65/399 (16%), Positives = 112/399 (27%), Gaps = 72/399 (18%)

Query: 7   LLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASR 66
           L F   +   F    ++F   F                     L  G    P    +   
Sbjct: 3   LFFALGLLIHFVFFASIFDIYF------------------TSPLVHGM--TPQFTPLPPP 42

Query: 67  FSRVVLVLIDALRFDFAQPQHSHRIL------EIQPHHARLYRSQVDPPTTTMQRLKALT 120
             R+VL + D LR D                  +  H      S    PT +     AL 
Sbjct: 43  AKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSWGVSHTRVPTESRPGHVALI 102

Query: 121 TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPG--------AFS 172
            G           +  + +  D+L  +           G      +F           +S
Sbjct: 103 AGFYEDVSAVAKGWKENPVEFDSLFNESKY----TWSWGSPDILPMFAKGASGDHVYTYS 158

Query: 173 KAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLGVDHCGH 220
                  F   D   +D  + + +    D+   +            V   H LG+D  GH
Sbjct: 159 YDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHLLGIDTNGH 218

Query: 221 KHGPHHPEMAKKLSQMDQVIQGLVERLEN------DTLLVVAGDHGMTTNGDHGGDSELE 274
            H P   E    + ++D  ++ +V   ++       T  +   DHGMT  G HG     E
Sbjct: 219 AHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHPSE 278

Query: 275 VSAALFLYSPTAVFPSTPPEEP----------------EVIPQVSLVPTLALLLGLPIPF 318
                  +     FP     +                   + Q  + P +A L+G+P P 
Sbjct: 279 TLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPLMASLIGVPFPL 338

Query: 319 GNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSR 357
            ++G +     +     +  S        L     ++++
Sbjct: 339 NSVGILPVGYLNNTGLFKAESMFTNAVQILEQFKVKMTQ 377
>dbj|BAA82620.1| (AB030280) phosphatidylinositolglycan class N short form [Mus
           musculus]
          Length = 798

 Score =  387 bits (986), Expect = e-106
 Identities = 75/465 (16%), Positives = 129/465 (27%), Gaps = 91/465 (19%)

Query: 7   LLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASR 66
           L F   +   F   +++F   F                     L  G    P    +   
Sbjct: 3   LFFALGLLIHFVFFVSIFDIYF------------------TSPLVHGM--TPQFTPLPPP 42

Query: 67  FSRVVLVLIDALRFDFAQPQHSHRIL------EIQPHHARLYRSQVDPPTTTMQRLKALT 120
             R+VL + D LR D                  +  H      S    PT +     AL 
Sbjct: 43  AKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSWGVSHTRVPTESRPGHVALI 102

Query: 121 TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPG--------AFS 172
            G           +  + +  D+L  +           G      +F           +S
Sbjct: 103 AGFYEDVSAVAKGWKENPVEFDSLFNESKY----TWSWGSPDILPMFAKGASGDHVYTYS 158

Query: 173 KAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLGVDHCGH 220
                  F   D   +D  + + +    D+   +            V   H LGVD  GH
Sbjct: 159 YDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHLLGVDTNGH 218

Query: 221 KHGPHHPEMAKKLSQMDQVIQGLVERLEN------DTLLVVAGDHGMTTNGDHGGDSELE 274
            H P   E    + ++D  ++ +V   ++       T  +   DHGMT  G HG     E
Sbjct: 219 AHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHPSE 278

Query: 275 VSAALFLYSPTAVFPSTPPEEP----------------EVIPQVSLVPTLALLLGLPIPF 318
                  +     FP     +                   + Q  + P +A L+G+P P 
Sbjct: 279 TLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPLMASLIGVPFPL 338

Query: 319 GNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQ 378
            ++G +     +     +  S        L     ++++              K+   L 
Sbjct: 339 NSVGILPVGYLNNTGLFKAESMFTNAVQILEQFKVKMTQ--------------KKEATLP 384

Query: 379 NLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIES 423
            LF+        LL   +  +          Q+      ++C E 
Sbjct: 385 FLFTPF-----KLLSDSQQLDILRKARSYIKQEKFDEVVSLCEEL 424
>ref|NP_038812.1| (NM_013784) phosphatidylinositol glycan, class N [Mus musculus]
 dbj|BAA82619.1| (AB030279) phosphatidylinositolglycan class N [Mus musculus]
          Length = 931

 Score =  385 bits (979), Expect = e-105
 Identities = 75/465 (16%), Positives = 129/465 (27%), Gaps = 91/465 (19%)

Query: 7   LLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASR 66
           L F   +   F   +++F   F                     L  G    P    +   
Sbjct: 3   LFFALGLLIHFVFFVSIFDIYF------------------TSPLVHGM--TPQFTPLPPP 42

Query: 67  FSRVVLVLIDALRFDFAQPQHSHRIL------EIQPHHARLYRSQVDPPTTTMQRLKALT 120
             R+VL + D LR D                  +  H      S    PT +     AL 
Sbjct: 43  AKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSWGVSHTRVPTESRPGHVALI 102

Query: 121 TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPG--------AFS 172
            G           +  + +  D+L  +           G      +F           +S
Sbjct: 103 AGFYEDVSAVAKGWKENPVEFDSLFNESKY----TWSWGSPDILPMFAKGASGDHVYTYS 158

Query: 173 KAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLGVDHCGH 220
                  F   D   +D  + + +    D+   +            V   H LGVD  GH
Sbjct: 159 YDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHLLGVDTNGH 218

Query: 221 KHGPHHPEMAKKLSQMDQVIQGLVERLEN------DTLLVVAGDHGMTTNGDHGGDSELE 274
            H P   E    + ++D  ++ +V   ++       T  +   DHGMT  G HG     E
Sbjct: 219 AHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHPSE 278

Query: 275 VSAALFLYSPTAVFPSTPPEEP----------------EVIPQVSLVPTLALLLGLPIPF 318
                  +     FP     +                   + Q  + P +A L+G+P P 
Sbjct: 279 TLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPLMASLIGVPFPL 338

Query: 319 GNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQ 378
            ++G +     +     +  S        L     ++++              K+   L 
Sbjct: 339 NSVGILPVGYLNNTGLFKAESMFTNAVQILEQFKVKMTQ--------------KKEATLP 384

Query: 379 NLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIES 423
            LF+        LL   +  +          Q+      ++C E 
Sbjct: 385 FLFTPF-----KLLSDSQQLDILRKARSYIKQEKFDEVVSLCEEL 424
>gb|AAH21148.1|AAH21148 (BC021148) Similar to phosphatidylinositol glycan, class N [Mus
           musculus]
          Length = 826

 Score =  384 bits (978), Expect = e-105
 Identities = 74/465 (15%), Positives = 128/465 (26%), Gaps = 91/465 (19%)

Query: 7   LLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASR 66
           L F   +   F    ++F   F                     L  G    P    +   
Sbjct: 3   LFFALGLLIHFVFFASIFDIYF------------------TSPLVHGM--TPQFTPLPPP 42

Query: 67  FSRVVLVLIDALRFDFAQPQHSHRIL------EIQPHHARLYRSQVDPPTTTMQRLKALT 120
             R+VL + D LR D                  +  H      S    PT +     AL 
Sbjct: 43  AKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIIHEGSWGVSHTRVPTESRPGHVALI 102

Query: 121 TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPG--------AFS 172
            G           +  + +  D+L  +           G      +F           +S
Sbjct: 103 AGFYEDVSAVAKGWKENPVEFDSLFNESKY----TWSWGSPDILPMFAKGASGDHVYTYS 158

Query: 173 KAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLGVDHCGH 220
                  F   D   +D  + + +    D+   +            V   H LG+D  GH
Sbjct: 159 YDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHLLGIDTNGH 218

Query: 221 KHGPHHPEMAKKLSQMDQVIQGLVERLEN------DTLLVVAGDHGMTTNGDHGGDSELE 274
            H P   E    + ++D  ++ +V   ++       T  +   DHGMT  G HG     E
Sbjct: 219 AHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHPSE 278

Query: 275 VSAALFLYSPTAVFPSTPPEEP----------------EVIPQVSLVPTLALLLGLPIPF 318
                  +     FP     +                   + Q  + P +A L+G+P P 
Sbjct: 279 TLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPLMASLIGVPFPL 338

Query: 319 GNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQ 378
            ++G +     +     +  S        L     ++++              K+   L 
Sbjct: 339 NSVGILPVGYLNNTGLFKAESMFTNAVQILEQFKVKMTQ--------------KKEATLP 384

Query: 379 NLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIES 423
            LF+        LL   +  +          Q+      ++C E 
Sbjct: 385 FLFTPF-----KLLSDSQQLDILRKARSYIKQEKFDEVVSLCEEL 424
>ref|NP_036459.1| (NM_012327) phosphatidylinositol glycan, class N; endoplasmic
           reticulum membrane protein [Homo sapiens]
 gb|AAD11432.1| (AF109219) Mcd4p homolog [Homo sapiens]
          Length = 931

 Score =  375 bits (953), Expect = e-102
 Identities = 63/399 (15%), Positives = 114/399 (27%), Gaps = 72/399 (18%)

Query: 7   LLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASR 66
           L F   +   F    ++F   F                     L  G    P    +   
Sbjct: 3   LFFTLGLLIHFVFFASIFDIYF------------------TSPLVHGM--TPQFTPLPPP 42

Query: 67  FSRVVLVLIDALRFDFAQPQHSHRIL------EIQPHHARLYRSQVDPPTTTMQRLKALT 120
             R+VL + D LR D       +          I  H      S    PT +     AL 
Sbjct: 43  ARRLVLFVADGLRADALYELDENGNSRAPFIRNIIMHEGSWGISHTRVPTESRPGHVALI 102

Query: 121 TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPG--------AFS 172
            G           +  + +  D+L  +           G      +F           +S
Sbjct: 103 AGFYEDVSAVAKGWKENPVEFDSLFNESKY----TWSWGSPDILPMFAKGASGDHVYTYS 158

Query: 173 KAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLGVDHCGH 220
                  F  +D   +D  + +++         +            V   H LG+D  GH
Sbjct: 159 YDAKREDFGAQDATKLDTWVFDNVKDFFHHARNNQSLFSKINEEKIVFFLHLLGIDTNGH 218

Query: 221 KHGPHHPEMAKKLSQMDQVIQGLVERLEN------DTLLVVAGDHGMTTNGDHGGDSELE 274
            H P   +    + ++D  ++ +V    +       T  +   DHGMT  G HG     E
Sbjct: 219 AHRPSSRDYKHNIKKVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHPSE 278

Query: 275 VSAALFLYSPTAVFPSTPPEEP----------------EVIPQVSLVPTLALLLGLPIPF 318
               L  +     +P     +                   + Q  + P +  L+G+P P 
Sbjct: 279 TLTPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWKRLDVNQADIAPLMTSLIGVPFPL 338

Query: 319 GNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSR 357
            ++G +  +  +  +  +  S        L     ++++
Sbjct: 339 NSVGILPVDYLNNTDLFKAESMFTNAVQILEQFKVKMTQ 377
>ref|NP_012756.1| (NC_001143) Required for GPI anchor synthesis; Mcd4p [Saccharomyces
           cerevisiae]
 sp|P36051|YKQ5_YEAST HYPOTHETICAL 105.7 KD PROTEIN IN TPK3-PIR1 INTERGENIC REGION
 pir||S37786 hypothetical protein YKL165c - yeast (Saccharomyces cerevisiae)
 emb|CAA81489.1| (Z26877) unknown [Saccharomyces cerevisiae]
 emb|CAA82007.1| (Z28165) ORF YKL165c [Saccharomyces cerevisiae]
 prf||2118404A ORF [Saccharomyces cerevisiae]
          Length = 919

 Score =  374 bits (952), Expect = e-102
 Identities = 77/474 (16%), Positives = 139/474 (29%), Gaps = 94/474 (19%)

Query: 3   KASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACW 62
           K    L    V F  +   ++F   F+                    L  G    P    
Sbjct: 4   KTRTTLLAVGVLFHLFYLWSIFDIYFI------------------SPLVHGM--SPYQST 43

Query: 63  MASRFSRVVLVLIDALRFDFAQPQHSHRIL-----------EIQPHHARLYRSQVDPPTT 111
                 R+ L++ D LR D    + +H +             +  ++A    S    PT 
Sbjct: 44  PTPPAKRLFLIVGDGLRADTTFDKVTHPVSGKTEFLAPFIRSLVMNNATYGISHTRMPTE 103

Query: 112 TMQRLKALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLF---- 167
           +     A+  G           + S+ +  D+   Q T         G      +F    
Sbjct: 104 SRPGHVAMIAGFYEDVSAVTKGWKSNPVNFDSFFNQSTH----TYSFGSPDILPMFKDGA 159

Query: 168 -----PGAFSKAFFFPSFNVRDLDTVDNGILEHLYPTMD------------SGEWDVLIA 210
                   +     F  F  +    +D  +  HL                   + +V   
Sbjct: 160 SDPNKVDTWMYDHTFEDF-TQSSIELDAFVFRHLDQLFHNSTLNSTLDYEIRQDGNVFFL 218

Query: 211 HFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERL-----ENDTLLVVAGDHGMTTNG 265
           H LG D  GH + P+  E    +  +D  I  L++++     ++ T  +   DHGM+  G
Sbjct: 219 HLLGCDTAGHSYRPYSAEYYDNVKYIDDQIPILIDKVNKFFADDKTAFIFTADHGMSAFG 278

Query: 266 DHGGDSELEVSAALFLYSPT--------------AVFPSTPPEEPE-VIPQVSLVPTLAL 310
            HG          L  +                               + Q  +   ++ 
Sbjct: 279 SHGDGHPNNTRTPLVAWGAGLNKPVHNPFPVSDNYTENWELSSIKRNDVKQADIASLMSY 338

Query: 311 LLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQ 370
           L+G+  P  ++GE+      G E          + +AL+ NA+ +            D Q
Sbjct: 339 LIGVNYPKNSVGELPIAYIDGKE--------SDKLAALYNNARSILEQYLVKQDEVIDSQ 390

Query: 371 AKELHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFL-RGARAMCIES 423
               ++    F + S  +            TL   I+E + +L + A  +  E 
Sbjct: 391 FF--YKEYFKFVEKSHSH---YLEEIE---TLIQRISEGENYLEQEAITLTEEL 436
>ref|NP_012473.1| (NC_001142) Local Anesthetics Sensitive: involved in the attachment
           of glycosylphosphatidylinositol (GPI) anchors to
           proteins.; Las21p [Saccharomyces cerevisiae]
 sp|P40367|YJG2_YEAST HYPOTHETICAL 94.9 KD PROTEIN IN MRPL8-NUP82 INTERGENIC REGION
 pir||S50810 probable membrane protein YJL062w - yeast (Saccharomyces
           cerevisiae)
 emb|CAA84061.1| (Z34288) HRC830 [Saccharomyces cerevisiae]
 emb|CAA89353.1| (Z49337) ORF YJL062w [Saccharomyces cerevisiae]
          Length = 830

 Score =  360 bits (915), Expect = 6e-98
 Identities = 133/431 (30%), Positives = 202/431 (46%), Gaps = 58/431 (13%)

Query: 17  FYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASRFSRVVLVLID 76
               I LF   F   ++ LT  S  Q+P     L                F ++V V+ID
Sbjct: 13  QLLAILLFIFAFFPRKIVLTGISK-QDPDQDRDLQR-----------DRPFQKLVFVIID 60

Query: 77  ALRFDFAQP---QHSHRIL-EIQPHHARLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGS 132
           ALR DF       H + +   +    A  Y S  +PPT T+ RLK++TTGS P+FID   
Sbjct: 61  ALRSDFLFDSQISHFNNVHQWLNTGEAWGYTSFANPPTVTLPRLKSITTGSTPSFIDLLL 120

Query: 133 N----FASHAIVE-DNLIKQLTSAGRRVVFMGDDTWKDLFPGA-FSKAFFFPSFNVRDLD 186
           N      S+ + E D+ ++Q       + FMGDDTW  LFP   F  A    SF V D  
Sbjct: 121 NVAQDIDSNDLSEHDSWLQQFIQHNNTIRFMGDDTWLKLFPQQWFDFADPTHSFFVSDFT 180

Query: 187 TVDNGILEHLYPTM--DSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLV 244
            VDN +  +L   +  +  +WDV I H+LG+DH GHK GPH   MA K  +MD +++ + 
Sbjct: 181 QVDNNVTRNLPGKLFQEWAQWDVAILHYLGLDHIGHKDGPHSKFMAAKHQEMDSILKSIY 240

Query: 245 ERL-----ENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEE---- 295
           + +     ++DTL+ V GDHGM   G+HGG S  E SA L   SP     + P  +    
Sbjct: 241 DEVLEHEDDDDTLICVLGDHGMNELGNHGGSSAGETSAGLLFLSPKLAQFARPESQVNYT 300

Query: 296 -------------PEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSAL 342
                         E + Q+ +VPT+A L G+PIP  ++G ++ +         P+  A 
Sbjct: 301 LPINASPDWNFQYLETVQQIDIVPTIAALFGMPIPMNSVGIIIPDFLQLL----PNKLAS 356

Query: 343 AQASALHLNAQQVSRFLHTYSAATQDLQAKE----LHQLQNLFSKASADYQWLLQSPKGA 398
            + + +HL   ++S   H    A  D  A++    ++ +Q   +K++ +Y + L +    
Sbjct: 357 MKENFMHL--WKLSD--HHGEVALDDFTAEDIYTKMYTIQETLTKSATNYNYPLLTLAFV 412

Query: 399 EATLPTVIAEL 409
              + T+IA  
Sbjct: 413 GFLIITIIAIY 423
>gb|AAF59557.2| (AC024812) Hypothetical protein Y54E10BR.1 [Caenorhabditis elegans]
          Length = 929

 Score =  352 bits (896), Expect = 1e-95
 Identities = 67/389 (17%), Positives = 127/389 (32%), Gaps = 63/389 (16%)

Query: 5   SVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMA 64
           S    +A +        ++F   +                     L  G    P     +
Sbjct: 2   SWKFLIASITVHLVLIYSIFDVYY------------------TSPLVHG---IPPQFINS 40

Query: 65  S--RFSRVVLVLIDALRFD-FAQPQHSHRILEIQPHH--ARLYRSQVDPPTTTMQRLKAL 119
                 R+ ++  D LR+D F +       L    +        S+   PT +     A+
Sbjct: 41  QEAPAKRIFIISADGLRYDTFNKYPDKSPYLHSIMNERKGIYGLSRSHIPTESRPGHVAI 100

Query: 120 TTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFP-----GAFSKA 174
             G           +  + +  D++  +  S        G     +LF       +FS +
Sbjct: 101 FAGITEDISAVAKGWKKNPVQFDSVFNRS-SYSW---MWGSPDIVNLFDDLPNAESFSYS 156

Query: 175 FFFPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLGVDHCGHKH 222
                F  +D   +D  + EH    +++ + D            +   H LG+D  GH +
Sbjct: 157 ADEEDFASKDASNLDKWVFEHFENFLETAKTDEALNDKMREQKSIFFLHLLGIDTNGHGN 216

Query: 223 GPHHPEMAKKLSQMDQVIQGL---VERL--ENDTLLVVAGDHGMTTNGDHGGDSELEVSA 277
            P   +    +  +D  I+ +   V+    ++ T  +   DHGMT  G HG  S+ EV  
Sbjct: 217 KPMSRQYIDNIKVVDSGIEKVQHLVDAFFGDHKTAWLFTSDHGMTDWGSHGAGSDDEVLT 276

Query: 278 ALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQP 337
               +                + Q+ L P ++ L+G PIP  ++G +  ++ +    S  
Sbjct: 277 PFVAWGAG----VKQGGPKLDLNQIDLAPLISALIGCPIPVNSMGILPVQMMNSKGSSYE 332

Query: 338 HSSALAQASALHLNAQQVSRFLHTYSAAT 366
                    A+  N +Q+   +     A 
Sbjct: 333 FK-------AIEANFKQLKEQIIFLKNAK 354
>ref|NP_491097.1| (NM_058696) Y54E10BR.1.p [Caenorhabditis elegans]
          Length = 348

 Score =  352 bits (895), Expect = 1e-95
 Identities = 67/378 (17%), Positives = 125/378 (32%), Gaps = 63/378 (16%)

Query: 5   SVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMA 64
           S    +A +        ++F   +                     L  G    P     +
Sbjct: 2   SWKFLIASITVHLVLIYSIFDVYY------------------TSPLVHG---IPPQFINS 40

Query: 65  S--RFSRVVLVLIDALRFD-FAQPQHSHRILEIQPHH--ARLYRSQVDPPTTTMQRLKAL 119
                 R+ ++  D LR+D F +       L    +        S+   PT +     A+
Sbjct: 41  QEAPAKRIFIISADGLRYDTFNKYPDKSPYLHSIMNERKGIYGLSRSHIPTESRPGHVAI 100

Query: 120 TTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFP-----GAFSKA 174
             G           +  + +  D++  +  S        G     +LF       +FS +
Sbjct: 101 FAGITEDISAVAKGWKKNPVQFDSVFNRS-SYSW---MWGSPDIVNLFDDLPNAESFSYS 156

Query: 175 FFFPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLGVDHCGHKH 222
                F  +D   +D  + EH    +++ + D            +   H LG+D  GH +
Sbjct: 157 ADEEDFASKDASNLDKWVFEHFENFLETAKTDEALNDKMREQKSIFFLHLLGIDTNGHGN 216

Query: 223 GPHHPEMAKKLSQMDQVIQGL---VERL--ENDTLLVVAGDHGMTTNGDHGGDSELEVSA 277
            P   +    +  +D  I+ +   V+    ++ T  +   DHGMT  G HG  S+ EV  
Sbjct: 217 KPMSRQYIDNIKVVDSGIEKVQHLVDAFFGDHKTAWLFTSDHGMTDWGSHGAGSDDEVLT 276

Query: 278 ALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQP 337
               +                + Q+ L P ++ L+G PIP  ++G +  ++ +    S  
Sbjct: 277 PFVAWGAG----VKQGGPKLDLNQIDLAPLISALIGCPIPVNSMGILPVQMMNSKGSSYE 332

Query: 338 HSSALAQASALHLNAQQV 355
                    A+  N +QV
Sbjct: 333 FK-------AIEANFKQV 343
>ref|NP_491750.1| (NM_059349) C27A12.9.p [Caenorhabditis elegans]
 gb|AAB93646.1| (AF003137) Hypothetical protein C27A12.9 [Caenorhabditis elegans]
          Length = 905

 Score =  343 bits (872), Expect = 6e-93
 Identities = 152/420 (36%), Positives = 223/420 (52%), Gaps = 66/420 (15%)

Query: 7   LLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGA-CWMAS 65
           ++ +  V       + +F +GFLL R E+ + SSC +          +  +PG  CWM  
Sbjct: 4   IIRILLVSINILLALFIFQNGFLLKRQEILSKSSCSD----------AHAQPGQTCWMKQ 53

Query: 66  RFSRVVLVLIDALRFDFAQPQHSHR----------------ILEIQPHHARLYRSQVDPP 109
           ++ RV+L+L+DALR+DF  P   +                    ++  +  +     DPP
Sbjct: 54  QYQRVILILVDALRYDFLIPIDENTKKSKEEWYYRGQMKNIEKLVKSGNVSIGTLLADPP 113

Query: 110 TTTMQRLKALTTGSLPTFIDAGSNFASHA-IVEDNLIKQLTSAGRRVVFMGDDTWKDLFP 168
           TTT+QRLKALTTG+LPTFIDAG NF+  A I ED+ + Q    G+ V  +GDDTW  LFP
Sbjct: 114 TTTLQRLKALTTGTLPTFIDAGDNFSPDAVISEDSFVYQAAQLGKNVTLLGDDTWLSLFP 173

Query: 169 GAFSKAFFFPSFNVRDLDTVDNGILEHLY------------------PTMDSGEWDVLIA 210
             FSK   + SF++ DL+TVD+ I   ++                    M +    ++IA
Sbjct: 174 NQFSKTAAYDSFDINDLNTVDDKIARKIFFGKYYNKMVFCFEGPILQDEMLNSNSSIIIA 233

Query: 211 HFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDHGMTTNGDHGGD 270
           HFLGVDHCGHK GP HP MA  L +MD++I   +E +++D       DHGMT+ GDHGG+
Sbjct: 234 HFLGVDHCGHKFGPSHPVMADTLRKMDRIIGQTIETMKSD-------DHGMTSTGDHGGE 286

Query: 271 SELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELF- 329
           SE E+ A + ++S         PE P  I Q+ +VPT++LL+GLPIPF N+G V+ +LF 
Sbjct: 287 SENEIRAGILVHSKKHQI--ILPERP--IHQIDIVPTISLLMGLPIPFSNLGTVITQLFT 342

Query: 330 -SGGEDSQPHSSALAQAS----ALHLNAQQVSRFLHTY---SAATQDLQAKELHQLQNLF 381
               E ++     + Q S    A    AQ+    LH++    + T + Q   + ++Q L 
Sbjct: 343 RDLWEIARQMMGFVWQDSVGIFAETYAAQKNFGELHSHTIRDSNTMEEQLDTMSRIQTLL 402
 Score = 40.5 bits (94), Expect = 0.12
 Identities = 22/44 (50%), Positives = 26/44 (59%), Gaps = 1/44 (2%)

Query: 746 ALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLV 789
           AL+    FY  GH PV  +I W+AAFVG P G  +   LPAL V
Sbjct: 755 ALLIPLGFYHLGHSPVISSIPWNAAFVGIPGGT-ALRVLPALFV 797
>gb|AAF58853.1| (AE003831) CG2292 gene product [Drosophila melanogaster]
          Length = 874

 Score =  339 bits (862), Expect = 9e-92
 Identities = 70/409 (17%), Positives = 135/409 (32%), Gaps = 61/409 (14%)

Query: 6   VLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMAS 65
            + +            ++F   F      +T  +     P    L +G +          
Sbjct: 2   WIAYAL--VVHVLLLGSIFVIYF--RSPVITGLT-----PQKHLLYYGLE---------P 43

Query: 66  RFSRVVLVLIDALRFDFAQPQHSHRILEIQP---HHARLYRSQVDPPTTTMQRLKALTTG 122
             +R+VL++ D  R D    ++   +  ++        +  S+   PT T      L  G
Sbjct: 44  PANRLVLIVTDGFRADSFFEENCRYVPNLRKIFLREGVVGVSRTRVPTETRPGHITLIAG 103

Query: 123 SLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFS------KAFF 176
                      + S+ I  D +  + +         G +   ++F    +      + + 
Sbjct: 104 LYEDPSAVLRGWKSNPIDFDTVFNRSSQ----TYAWGANDVLNVFSHVSNGGEINLRFYN 159

Query: 177 FPSFNVR--DLDTVDNGILEHLY-------PTMDSGEWDVLIAHFLGVDHCGHKHGPHHP 227
                    D    D  + + +          +   +  V   H LG+D  GH H P  P
Sbjct: 160 HDLDFSPGYDAYEEDEWVFKRVKLLLQQKREALQRAQNVVFFLHLLGLDTAGHVHKPGAP 219

Query: 228 EMAKKLSQMDQVIQGLVERL-----ENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLY 282
           +  + L + ++ +  + +       +  T  ++  DHGMT +G     S          +
Sbjct: 220 KFRRTLEKTEKGVYAIYQEFERVFPDKRTAYLLTADHGMTDSGA----SRAVPKPGGRTF 275

Query: 283 SPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSAL 342
            P    P+ P  E E   Q  L P ++ LLGL  P  N G++     +  ++        
Sbjct: 276 MPNNEGPAMPLHELE---QAQLTPLMSALLGLAPPMNNFGKLPLGYLNVSKEY------- 325

Query: 343 AQASALHLNAQQVSRFL-HTYSAATQDLQAKELHQLQNLFSKASADYQW 390
            +A A H+NA Q+            +   A  L +   L ++A   Y+ 
Sbjct: 326 -EAMAAHVNALQLLEQYVALQKQHKKGFFADILLEYDVLNAEAIKRYKD 373
>pir||T40715 hypothetical protein SPBC839.08c - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA17011.1| (AL021816) hypothetical protein [Schizosaccharomyces pombe]
 emb|CAB46701.1| (AL096796) hypothetical protein [Schizosaccharomyces pombe]
          Length = 935

 Score =  334 bits (848), Expect = 4e-90
 Identities = 71/414 (17%), Positives = 125/414 (30%), Gaps = 88/414 (21%)

Query: 8   LFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASRF 67
           L L  + F      ++F   F               P   G   + +   P         
Sbjct: 5   LLLLGILFHVVFLKSIFDIYF-------------VTPLIHGMKQYSAGEAP--------A 43

Query: 68  SRVVLVLIDALRFDFAQPQHSHRIL------------EIQPHHARLYRSQVDPPTTTMQR 115
            R+ L++ D LR D     HS +++             I  ++     S    PT +   
Sbjct: 44  KRLFLIVGDGLRPDKLLQPHSEKVIGEEQTYAAPFLRSIIQNNGTFGVSHTRVPTESRPG 103

Query: 116 LKALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPG------ 169
             AL  G           +  + +  D++  Q           G +    +F        
Sbjct: 104 HVALIAGFYEDVSAVTKGWKKNPVNFDSVFNQSRH----TYSFGSEDILPMFSEGASDPS 159

Query: 170 ---AFSKAFFFPSFNVRDLDTVDNGILEHLYPTM-----DSGEWD-------VLIAHFLG 214
               F  +     F+   +  +D  + + L   +     D   WD       V   H LG
Sbjct: 160 RVDTFMYSSELEDFSSNGIV-LDEWVFDRLDELLAQSLEDKELWDMLHRDKIVFFLHLLG 218

Query: 215 VDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTL------LVVAGDHGMTTNGDHG 268
           +D  GH   P   E  + +  +D  IQ LV+++ N          V   DHGM+  G HG
Sbjct: 219 IDTIGHNKHPDSVEYVENIQYIDGKIQELVDKMNNYYNNDGASSWVFTADHGMSDFGSHG 278

Query: 269 GDSELEVSAALFLYSPTAV--------------FPSTPPEEPE-VIPQVSLVPTLALLLG 313
             +       +  +                    P    E     I Q  +   ++ L+G
Sbjct: 279 DGNLDNTRTPIIAWGAGIQSPTHEKNYGHDEYSLPWNLTEIKRIDIQQADIAALMSYLVG 338

Query: 314 LPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQ 367
           L  P  ++G++  +                +A    +NA ++    +  SA+  
Sbjct: 339 LNFPVNSVGQIPLDYLDCSS--------RRKAEVALMNALEIGEQYNLKSASKD 384
>dbj|BAB29186.1| (AK014165) data source:MGD, source key:MGI:1351629,
           evidence:ISS~phosphatidylinositol glycan, class
           N~putative [Mus musculus]
          Length = 320

 Score =  333 bits (846), Expect = 7e-90
 Identities = 55/324 (16%), Positives = 88/324 (26%), Gaps = 56/324 (17%)

Query: 7   LLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASR 66
           L F   +   F    ++F   F                     L  G    P    +   
Sbjct: 3   LFFALGLLIHFVFFASIFDIYF------------------TSPLVHGM--TPQFTPLPPP 42

Query: 67  FSRVVLVLIDALRFDFAQPQHSHRIL------EIQPHHARLYRSQVDPPTTTMQRLKALT 120
             R+VL + D LR D                  +  H      S    PT +     AL 
Sbjct: 43  AKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSWGVSHTRVPTESRPGHVALI 102

Query: 121 TGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPG--------AFS 172
            G           +  + +  D+L  +           G      +F           +S
Sbjct: 103 AGFYEDVSAVAKGWKENPVESDSLFNERKY----TWSWGSPDILPMFAKGASGDHVYTYS 158

Query: 173 KAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLGVDHCGH 220
                  F   D   +D  + + +    D+   +            V   H LG+D  GH
Sbjct: 159 YDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHLLGIDTNGH 218

Query: 221 KHGPHHPEMAKKLSQMDQVIQGLVERLEN------DTLLVVAGDHGMTTNGDHGGDSELE 274
            H P   E    + ++D  ++ +V   ++       T  +   DHGMT  G HG     E
Sbjct: 219 AHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHPSE 278

Query: 275 VSAALFLYSPTAVFPSTPPEEPEV 298
                  +     FP     +   
Sbjct: 279 TLTPFVTWGAGIKFPQNVSAQQYD 302
>ref|NP_186787.1| (NM_111004) putative phosphatidylinositolglycan class N short form
           [Arabidopsis thaliana]
 gb|AAF24617.1|AC010870_10 (AC010870) putative phosphatidylinositolglycan class N short form
           [Arabidopsis thaliana]
          Length = 921

 Score =  328 bits (833), Expect = 2e-88
 Identities = 79/521 (15%), Positives = 143/521 (27%), Gaps = 126/521 (24%)

Query: 2   QKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGAC 61
           ++ + L+    V       +++F   F                     +  G    P   
Sbjct: 41  RRETWLVV-LGVALHAVYMLSIFDIYF------------------KTPIVHGMDPVPPRF 81

Query: 62  WMASRFSRVVLVLIDALRFDFAQPQHSHRIL------EIQPHHARLYRSQVDPPTTTMQR 115
                  R+VL++ D LR D                  I  +  R   S   PPT +   
Sbjct: 82  S-EPPAKRLVLLISDGLRADKFFEPDEEGKYRAPFLRNIIKNQGRWGVSHARPPTESRPG 140

Query: 116 LKALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTS---AGRR------VVFMGDDTWKDL 166
             A+  G           + ++ +  D++  Q       G           +   TW   
Sbjct: 141 HVAIIAGFYEDPSAVTKGWKANPVEFDSVFNQSRHTFAFGSPDIIPIFCSALPHSTWNS- 199

Query: 167 FPGAFSKAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLG 214
           +P  +       SF       +D    +     ++    D            V+  H LG
Sbjct: 200 YPHEYEDFATDASF-------LDEWSFDQFEGLLNRSHADPKLKELLHQDKLVVFLHLLG 252

Query: 215 VDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERL-----ENDTLLVVAGDHGMTTNGDHGG 269
            D  GH H P+       +  +D++ + +   L     +N T  +   DHGM+  G HG 
Sbjct: 253 CDSNGHAHRPYSSIYLNNVKVVDKIAERVYHLLEDYYRDNRTSYIFTADHGMSDKGSHGD 312

Query: 270 DSELEVSAALFLYSPTAVFPS-----------------------TPPEEPEV------IP 300
                    L  +     +P                        TP +          + 
Sbjct: 313 GHPTNTDTPLVAWGAGIQYPKPASGNSHSDSVTTFVDKHAHDMPTPYDWGLRRVERVDVN 372

Query: 301 QVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLH 360
           Q  + P ++ LLGLP P  ++G +               +   +  A+  N +Q+   L 
Sbjct: 373 QADIAPLMSTLLGLPCPVNSVGNLPLGYMKL--------NEAEEVEAVVANTKQILNQLL 424

Query: 361 TYSAATQDLQ------------AKELHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAE 408
             S                   +  L Q+  L S  S       ++       L  +  E
Sbjct: 425 RKSYIKSSNSLFFKPFKPLVHHSFSLSQIDELISAKS------YEAAMKLAVDLRNLSLE 478

Query: 409 LQQFLRGARAMCIESWARFSLVRMAGAKNLWYGACVAALVA 449
              + +            +  + +     L Y   +  L  
Sbjct: 479 GLHYFQT-----------YDWLMLMTVITLGYTGWMIVLAL 508
 Score =  293 bits (744), Expect = 8e-78
 Identities = 53/321 (16%), Positives = 93/321 (28%), Gaps = 60/321 (18%)

Query: 2   QKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGAC 61
           ++ + L+    V       +++F   F                     +  G    P   
Sbjct: 41  RRETWLVV-LGVALHAVYMLSIFDIYF------------------KTPIVHGMDPVPPRF 81

Query: 62  WMASRFSRVVLVLIDALRFDFAQPQHSHRIL------EIQPHHARLYRSQVDPPTTTMQR 115
                  R+VL++ D LR D                  I  +  R   S   PPT +   
Sbjct: 82  S-EPPAKRLVLLISDGLRADKFFEPDEEGKYRAPFLRNIIKNQGRWGVSHARPPTESRPG 140

Query: 116 LKALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTS---AGRR------VVFMGDDTWKDL 166
             A+  G           + ++ +  D++  Q       G           +   TW   
Sbjct: 141 HVAIIAGFYEDPSAVTKGWKANPVEFDSVFNQSRHTFAFGSPDIIPIFCSALPHSTWNS- 199

Query: 167 FPGAFSKAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLG 214
           +P  +       SF       +D    +     ++    D            V+  H LG
Sbjct: 200 YPHEYEDFATDASF-------LDEWSFDQFEGLLNRSHADPKLKELLHQDKLVVFLHLLG 252

Query: 215 VDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERL-----ENDTLLVVAGDHGMTTNGDHGG 269
            D  GH H P+       +  +D++ + +   L     +N T  +   DHGM+  G HG 
Sbjct: 253 CDSNGHAHRPYSSIYLNNVKVVDKIAERVYHLLEDYYRDNRTSYIFTADHGMSDKGSHGD 312

Query: 270 DSELEVSAALFLYSPTAVFPS 290
                    L  +     +P 
Sbjct: 313 GHPTNTDTPLVAWGAGIQYPK 333
>sp|Q09782|YA93_SCHPO HYPOTHETICAL 85.7 KD PROTEIN C13G6.03 IN CHROMOSOME I
 pir||S62432 major facilitator protein homolog - fission yeast
           (Schizosaccharomyces pombe)
 pir||T37638 major facilitator protein homolog - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA91096.1| (Z54308) putative major facilitator superfamily protein
           [Schizosaccharomyces pombe]
          Length = 758

 Score =  323 bits (821), Expect = 6e-87
 Identities = 127/392 (32%), Positives = 182/392 (46%), Gaps = 52/392 (13%)

Query: 8   LFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASRF 67
                + +L   G  LF  GF   + + T  +   +   P  +                 
Sbjct: 3   FAFFGIFWLQIFGSILFLLGFFPHKNDSTGKAMSNQFSPPAVI----------------- 45

Query: 68  SRVVLVLIDALRFDFAQPQHSHRIL-----EIQPHHARLYRSQVDPPTTTMQRLKALTTG 122
            +VV V++DALR DF   + SH +           H   + +    PT TM RLKALTTG
Sbjct: 46  DQVVFVMVDALRADFVFSK-SHNMPFTQSLLYNSTHGIGFSAFARSPTVTMPRLKALTTG 104

Query: 123 SLPTFIDAGSNFASHAIV-----EDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFF 177
           ++P F+D   N A          +D+ + QL S  +++ F GDDTW  LFP AFSK    
Sbjct: 105 TIPGFLDVLLNIAESDTGSSIEAQDSWVYQLNSFNKKIEFYGDDTWLKLFPSAFSKFEGT 164

Query: 178 PSFNVRDLDTVDNGI---LEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLS 234
            SF V D   VDN +    +H  P+  S  WD LI H+LGVDH GH +GP  P +  KL 
Sbjct: 165 TSFFVSDYTEVDNNVTRNFDHALPSSLSHSWDALILHYLGVDHIGHLYGPSSPLLNIKLL 224

Query: 235 QMDQVIQGLV-------ERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAV 287
           ++D +I  +        E+    +L+V+ GDHGM   G+HGG S  E +AAL L  P+  
Sbjct: 225 EIDTIISRIYKYLQEYDEKTNTHSLIVLCGDHGMNEVGNHGGSSSGETTAALSLLFPSNE 284

Query: 288 FP----------STPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQP 337
                         P    E + QV +VPT+ LLLG+PIP GN+G+V++ +    +D++ 
Sbjct: 285 LSHINKPILNMDDNPYSILERVEQVDVVPTICLLLGIPIPKGNMGKVLSPVTELWKDTKT 344

Query: 338 HSSA----LAQASALHLNAQQVSRFLHTYSAA 365
              A    L Q S L   +   S     +  +
Sbjct: 345 AKMAALSNLFQLSLLKNPSLTTSELSTQFQDS 376
>ref|XP_056208.1| (XM_056208) hypothetical protein MGC3079 [Homo sapiens]
 gb|AAH13987.1|AAH13987 (BC013987) Similar to hypothetical protein MGC3079 [Homo sapiens]
          Length = 454

 Score =  304 bits (772), Expect = 4e-81
 Identities = 227/227 (100%), Positives = 227/227 (100%)

Query: 200 MDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDH 259
           MDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDH
Sbjct: 1   MDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDH 60

Query: 260 GMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFG 319
           GMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFG
Sbjct: 61  GMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFG 120

Query: 320 NIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQN 379
           NIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQN
Sbjct: 121 NIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQN 180

Query: 380 LFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWAR 426
           LFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWAR
Sbjct: 181 LFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWAR 227
 Score =  266 bits (675), Expect = 1e-69
 Identities = 140/144 (97%), Positives = 141/144 (97%)

Query: 731 TPCPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVG 790
           TP PFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVG
Sbjct: 246 TPGPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVG 305

Query: 791 ANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEEEPLMEMRLRD 850
           ANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEEEPLMEMRLRD
Sbjct: 306 ANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEEEPLMEMRLRD 365

Query: 851 APQHFYAALLQLGLKYLFILGSYL 874
           APQHFYAALLQLGLKYLFILG  +
Sbjct: 366 APQHFYAALLQLGLKYLFILGIQI 389
>ref|NP_116023.1| (NM_032634) hypothetical protein MGC3079 [Homo sapiens]
 gb|AAH01030.1|AAH01030 (BC001030) Similar to phosphatidylinositol glycan, class O [Homo
           sapiens]
          Length = 454

 Score =  298 bits (756), Expect = 3e-79
 Identities = 222/227 (97%), Positives = 223/227 (97%)

Query: 200 MDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDH 259
           MDSGEW+V   H LGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDH
Sbjct: 1   MDSGEWEVSNQHVLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDH 60

Query: 260 GMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFG 319
           GMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFG
Sbjct: 61  GMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFG 120

Query: 320 NIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQN 379
           NIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQN
Sbjct: 121 NIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQN 180

Query: 380 LFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWAR 426
           LFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWAR
Sbjct: 181 LFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWAR 227
 Score =  266 bits (675), Expect = 8e-70
 Identities = 140/144 (97%), Positives = 141/144 (97%)

Query: 731 TPCPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVG 790
           TP PFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVG
Sbjct: 246 TPGPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVG 305

Query: 791 ANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEEEPLMEMRLRD 850
           ANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEEEPLMEMRLRD
Sbjct: 306 ANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEEEPLMEMRLRD 365

Query: 851 APQHFYAALLQLGLKYLFILGSYL 874
           APQHFYAALLQLGLKYLFILG  +
Sbjct: 366 APQHFYAALLQLGLKYLFILGIQI 389
>gb|AAF56048.1| (AE003740) CG4907 gene product [Drosophila melanogaster]
          Length = 919

 Score =  285 bits (723), Expect = 2e-75
 Identities = 60/380 (15%), Positives = 119/380 (30%), Gaps = 68/380 (17%)

Query: 13  VCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASRFSRVVL 72
           +        ++ +  F  T L      S     G                +     R+V+
Sbjct: 7   LVVHLLLLGSILSIYFQSTILSDLEPLSTLRELG----------------LEPPADRLVV 50

Query: 73  VLIDALRFDFAQPQHSHRILEIQP---HHARLYRSQVDPPTTTMQRLKALTTGSLPTFID 129
            ++D LR       H   + +++      A +  S+  PPT T     A+  G       
Sbjct: 51  FVVDGLRAQSVLADHCSAVPDLRELFVEQALVGISRACPPTVTRPGHIAIFGGFNEDP-A 109

Query: 130 AGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFS-----KAFFFPSFNVRD 184
           A   +  +    D++  +     R  +         +F    +     +   F   ++ D
Sbjct: 110 ATLTW--NPSTFDSVFNRS----RNAIGWMQAEVAKVFTHLPTGGAPLRFETFARSDISD 163

Query: 185 LDTVDNGILEHLYPTMDSGEW---------DVLIAHFLGVDHCGHKHGPHHPEMAKKLSQ 235
              +D    + +   M + +           +   +   +D   H H P+     +KL+ 
Sbjct: 164 RLRLDQWTFDKVRNFMTNEQNVQPLRDATSVIFFVYLADIDLAEHVHMPNSLNFREKLNY 223

Query: 236 MDQVIQGLVERL-----ENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAV--- 287
             + I+   E       ++ T  ++  D+G++    HGG  E  V     L+        
Sbjct: 224 TQRGIRQTYELFESVFNDSRTAYLMTADYGISL---HGGGGERGVETPFILWGAGVKRSA 280

Query: 288 ---------FPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPH 338
                      + P      + Q+ L P ++ L+GLP P  N+  +     +        
Sbjct: 281 PNPGQNFTAGENGPILPLYQLEQIQLAPLMSALIGLPPPMNNMALMPLGFLNTSVQY--- 337

Query: 339 SSALAQASALHLNAQQVSRF 358
                +   LHLNA Q+   
Sbjct: 338 -----ELQVLHLNAMQLLAQ 352
>pir||T46311 hypothetical protein DKFZp434H0711.1 - human (fragment)
 emb|CAB70839.1| (AL137607) hypothetical protein [Homo sapiens]
          Length = 825

 Score =  252 bits (639), Expect = 1e-65
 Identities = 40/275 (14%), Positives = 83/275 (29%), Gaps = 46/275 (16%)

Query: 125 PTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPG--------AFSKAFF 176
                    +  + +  D+L  +           G      +F           +S    
Sbjct: 1   EDVSAVAKGWKENPVEFDSLFNESKY----TWSWGSPDILPMFAKGASGDHVYTYSYDAK 56

Query: 177 FPSFNVRDLDTVDNGILEHLYPTMDSGEWD------------VLIAHFLGVDHCGHKHGP 224
              F  +D   +D  + +++         +            V   H LG+D  GH H P
Sbjct: 57  REDFGAQDATKLDTWVFDNVKDFFHHARNNQSLFSKINEEKIVFFLHLLGIDTNGHAHRP 116

Query: 225 HHPEMAKKLSQMDQVIQGLVERLEN------DTLLVVAGDHGMTTNGDHGGDSELEVSAA 278
              +    + ++D  ++ +V    +       T  +   DHGMT  G HG     E    
Sbjct: 117 SSRDYKHNIKKVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHPSETLTP 176

Query: 279 LFLYSPTAVFPSTPPEEP----------------EVIPQVSLVPTLALLLGLPIPFGNIG 322
           L  +     +P     +                   + Q  + P +  L+G+P P  ++G
Sbjct: 177 LVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWKRLDVNQADIAPLMTSLIGVPFPLNSVG 236

Query: 323 EVMAELFSGGEDSQPHSSALAQASALHLNAQQVSR 357
            +  +  +  +  +  S        L     ++++
Sbjct: 237 ILPVDYLNNTDLFKAESMFTNAVQILEQFKVKMTQ 271
>ref|NP_281901.1| (NC_002163) hypothetical protein Cj0729 [Campylobacter jejuni]
 pir||D81344 hypothetical protein Cj0729 [imported] - Campylobacter jejuni
           (strain NCTC 11168)
 emb|CAB73003.1| (AL139076) hypothetical protein Cj0729 [Campylobacter jejuni]
          Length = 269

 Score =  236 bits (598), Expect = 1e-60
 Identities = 58/263 (22%), Positives = 106/263 (40%), Gaps = 27/263 (10%)

Query: 68  SRVVLVLIDALRFDFAQPQHSHRILEIQPHHARLYRSQVDPPTTTMQRLKALTTGSLPTF 127
           S+ +LV++D L    A     +     + +  + Y  + + P+ +    + L TG  P  
Sbjct: 3   SKAILVVLDGLNCKSASINMGYLNALCKENLGKFYSLECELPSMSRPLYECLLTGVKPVL 62

Query: 128 IDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSF--NVRDL 185
               +N  S +  + ++       G +    G   +  +F   ++K  F PS   ++ D 
Sbjct: 63  SGIINNKLSFS-KQTSIFDLCKEQGLK---AGGAAYHWVF-ELYNKKEFIPSLHRHIEDE 117

Query: 186 D-TVDNGILEHLYPTMDSG------------EWDVLIAHFLGVDHCGHKHGPHHPEMAKK 232
           + T+  G   +    +DS               D  + H + +D  GHK G H  E A K
Sbjct: 118 NLTLPYGHFYYEDDYLDSHLFADGEHLRNKYNLDFTLIHSMNIDDAGHKFGSHSIEYANK 177

Query: 233 LSQMDQVI-QGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVFPST 291
             ++D +I + L   LE    +++  DHGMT    HGG SE E+    F +     + + 
Sbjct: 178 TKKVDILISEYLPTWLEQGINVIITSDHGMTEGKSHGGLSEDEILVPFFTFGSAFSYENA 237

Query: 292 PPEEPEVIPQVSLVPTLALLLGL 314
             ++ E+   +        +LGL
Sbjct: 238 KIKQDEICGSI------CEILGL 254
>dbj|BAB17014.1| (AB050262) hypothetical protein [Macaca fascicularis]
          Length = 333

 Score =  206 bits (521), Expect = 1e-51
 Identities = 67/242 (27%), Positives = 108/242 (43%), Gaps = 26/242 (10%)

Query: 207 VLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLEND-------TLLVVAGDH 259
           +LI H+LG+DH GH  GP+ P +  KLS+MD V+  +   L++         LLV+ GDH
Sbjct: 1   MLILHYLGLDHIGHISGPNSPLIGHKLSEMDSVLMKIHTSLQSKERETPLPNLLVLCGDH 60

Query: 260 GMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFG 319
           GM+  G HG  S  EV+  L L S            P+ + Q  +  TLA+ LGLPIP  
Sbjct: 61  GMSETGSHGASSMEEVNTPLILISSAFERKPGDIRHPKHVQQTDVAATLAIALGLPIPKD 120

Query: 320 NIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTY--SAATQ-DLQAKELHQ 376
           ++G ++  +  G    +P      Q   LHLN  Q+S+ L     S       +  ++ +
Sbjct: 121 SVGSLLFPVVEG----RPMRE---QLRFLHLNTVQLSKLLQENVPSYEKDPGFEQFKMSE 173

Query: 377 LQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESW---ARFSLVRMA 433
                 +   ++  L    K +E         L+Q+L   + + +      A++ +  M 
Sbjct: 174 ------RLHGNWIRLYLEEKHSEVLFNLGSKVLRQYLAALKTLSLSLSTQVAQYDMYSMM 227

Query: 434 GA 435
             
Sbjct: 228 VG 229
>gb|AAF56711.1| (AE003761) CG13978 gene product [Drosophila melanogaster]
          Length = 475

 Score =  196 bits (494), Expect = 1e-48
 Identities = 33/236 (13%), Positives = 63/236 (25%), Gaps = 39/236 (16%)

Query: 6   VLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMAS 65
             L             A+  + F      L N       P       G +          
Sbjct: 2   WKLQAL--VVHILLMGAILNTYF--QHTLLPNL-----VPQKTMRELGLE---------P 43

Query: 66  RFSRVVLVLIDALRFDFAQPQHSHRILEIQP---HHARLYRSQVDPPTTTMQRLKALTTG 122
              R+V+ + D LR       +   + +++       R+  S+   PT T     A+  G
Sbjct: 44  PADRLVVFVTDGLRAATFLANNGSDVPDLKDIYRQQGRIGISRTCAPTMTRPGHIAIFAG 103

Query: 123 SLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFF--FPSF 180
                  +  +   +    D +  +     R ++          F           F S+
Sbjct: 104 FHEDPAASLMHLCYNPGDFDTVFNRS----RNMIGWAHSYIVGYFVKLSHGGAPLRFDSY 159

Query: 181 NVRDL---DTVDNGILEHLYPTMDSGEWD---------VLIAHFLGVDHCGHKHGP 224
             RDL    T D    + +   + + +           V   +   +D   H+  P
Sbjct: 160 MERDLPEKLTCDKWAFDKVENFLRNVDNVREWRNYKPAVFFVYLADMDIAAHRFKP 215
 Score =  104 bits (259), Expect = 6e-21
 Identities = 38/208 (18%), Positives = 72/208 (34%), Gaps = 26/208 (12%)

Query: 257 GDHGMTTNGDHGGDSELEVSAALFLYSPT-----AVFPSTPPEEPEV--IPQVSLVPTLA 309
             H       HG  S LEV   LF++            +  PE+P +  + Q  L P ++
Sbjct: 209 AAHRFKPLRAHGSGSPLEVETPLFMWGAGVKRDEIDAEANFPEKPNISQVDQTQLAPLMS 268

Query: 310 LLLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRF--LHTYSAATQ 367
            L+GLP P  N+  +     +  ++         QA ALHLN  Q+     +        
Sbjct: 269 SLIGLPPPKNNLALMPVGYLNVSDEY--------QAVALHLNVLQLLSQAEILIGRHEKA 320

Query: 368 DLQAKELHQLQNLFSKASADYQWLL---QSPKGAEATLPTVIAELQQFLRGARAMCIESW 424
                  ++    F +                 +E  L   +   +++ + A+  C+  +
Sbjct: 321 IF-----YKWLPKFKELHIGVIDQYAAQFDILKSEGNLTDAMEFCKEYGKLAKK-CLTYY 374

Query: 425 ARFSLVRMAGAKNLWYGACVAALVALLA 452
            ++    +  A ++ Y      L+  L 
Sbjct: 375 NQYYQTPLIVANSMSYMIWFYCLLLQLT 402
>ref|NP_251941.1| (NC_002516) hypothetical protein [Pseudomonas aeruginosa]
 pir||E83240 hypothetical protein PA3251 [imported] - Pseudomonas aeruginosa
           (strain PAO1)
 gb|AAG06639.1|AE004747_12 (AE004747) hypothetical protein [Pseudomonas aeruginosa]
          Length = 269

 Score =  191 bits (482), Expect = 4e-47
 Identities = 58/267 (21%), Positives = 95/267 (34%), Gaps = 39/267 (14%)

Query: 70  VVLVLIDALRFDFAQPQHSHRILEIQPHHARLYRSQVDPPTTTMQRLKALTTGSLPTFID 129
           V+LVL+D L    A+    H          + YR + + P+ +    + + TG  P    
Sbjct: 5   VILVLLDGLNHSVARDCMGHLQALCGAGRGQSYRLECELPSLSRPLYECILTGVPP---- 60

Query: 130 AGSNFASHAI----VEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSFNVRDL 185
             S    + +     E +L     +AG          W       +++A F P    RD 
Sbjct: 61  IDSGILHNDVVRLSQEQSLFHYARAAGLSTAAAAYH-WVS---ELYNRAPFDP---ARDR 113

Query: 186 DTVD------NGIL---EHLYPTM---------DSGEWDVLIAHFLGVDHCGHKHGPHHP 227
            T D       G     +H   +               + ++ H + +D  GH+HG   P
Sbjct: 114 HTDDSALPIQRGHFYWSDHYPDSHLFADAESLRRRHAPNFMLVHSMNIDDAGHRHGLASP 173

Query: 228 EMAKKLSQMDQVI-QGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTA 286
                  + D ++ + L + L     + +  DHGM  +  HGG  E E    LF++    
Sbjct: 174 AYRNAARRADALLSEYLHQWLAAGYQVAITADHGMNEDRSHGGILEEEREVPLFVFGDA- 232

Query: 287 VFPSTPPEEPEVIPQVSLVPTLALLLG 313
            F      +P    Q  L  TL  +LG
Sbjct: 233 -FSRDDLAQPL---QTDLCGTLCEILG 255
>pir||T33724 hypothetical protein - Zymomonas mobilis
 gb|AAC70363.1| (AF086791) unknown [Zymomonas mobilis]
          Length = 429

 Score =  188 bits (473), Expect = 4e-46
 Identities = 46/222 (20%), Positives = 76/222 (33%), Gaps = 27/222 (12%)

Query: 70  VVLVLIDALRFDFAQPQHSHRILEIQPHHARLYRSQVDPPTTTMQRLKALTTGSLPTFID 129
           ++L+ ID  R D+ +   +  ++ +  + +         P+ T      L TG  P    
Sbjct: 50  LILISIDGFRADYIKRGLTPNLVSLAENGSYAKVMHPSFPSITFPNHYTLVTGLYPDHHG 109

Query: 130 -AGSNFASHAIVEDNLIKQLTSAGRRVVFM---GDDTW-----------KDLFPGAFSKA 174
             G+N     I  D+  K               G+  W              +PG+ +  
Sbjct: 110 IVGNNMDDAHITPDSHFKMSDHQAATDRRWWDEGEPLWVTAEKQGVVSATMFWPGSEADI 169

Query: 175 FFFP-------SFNVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHP 227
                        +V   + VD  +   L           +  +F  VDH GH +GP   
Sbjct: 170 EGVRPEMWRQFDSHVPFSERVDQ-VFSWL-AYPKKKRPQFITLYFENVDHAGHLYGPDSQ 227

Query: 228 EMAKKLSQMDQVIQGLVERLENDT---LLVVAGDHGMTTNGD 266
           E+   L ++DQ I  LV+ L+       LV+  DHGM     
Sbjct: 228 EVNDNLVKIDQTIGQLVQGLKQRGIKANLVIVSDHGMAATSS 269
>dbj|BAA87261.1| (AB027957) Hypothetical protein [Schizosaccharomyces pombe]
          Length = 179

 Score =  177 bits (446), Expect = 6e-43
 Identities = 37/182 (20%), Positives = 63/182 (34%), Gaps = 29/182 (15%)

Query: 207 VLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTL------LVVAGDHG 260
           V   H LG+D  GH   P   E  + +  +D  IQ LV+++ N          V   DHG
Sbjct: 6   VFFLHLLGIDTIGHNKHPDSVEYVENIQYIDGKIQELVDKMNNYYNNDGASSWVFTADHG 65

Query: 261 MTTNGDHGGDSELEVSAALFLYSPTAV--------------FPSTPPEEPE-VIPQVSLV 305
           M+  G HG  +       +  +                    P    E     I Q  + 
Sbjct: 66  MSDFGSHGDGNLDNTRTPIIAWGAGIQSPTHEKNYGHDEYSLPWNLTEIKRIDIQQADIA 125

Query: 306 PTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAA 365
             ++ L+GL  P  ++G++  +                +A    +NA ++    +  SA+
Sbjct: 126 ALMSYLVGLNFPVNSVGQIPLDYLDCSS--------RRKAEVALMNALEIGEQYNLKSAS 177

Query: 366 TQ 367
             
Sbjct: 178 KD 179
>dbj|BAB29052.1| (AK013913) data source:MGD, source key:MGI:1861452,
           evidence:ISS~phosphatidylinositol glycan, class
           O~putative [Mus musculus]
          Length = 171

 Score =  175 bits (440), Expect = 3e-42
 Identities = 84/106 (79%), Positives = 93/106 (87%), Gaps = 3/106 (2%)

Query: 772 VGFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEAD 831
           VGFP+GHGS TWLPALLVGANTFASHLLFAVGCPLLLLWPFLCE QG ++RQ  PG+E++
Sbjct: 1   VGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESE 60

Query: 832 ARVR---PEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGSYL 874
           ARVR    EE +EPLMEMRLRDAP HF AALLQLGLKYLFILG+ +
Sbjct: 61  ARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQI 106
>ref|NP_391271.1| (NC_000964) phosphoglycerate mutase [Bacillus subtilis]
 sp|P39773|PMGI_BACSU 2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHOGLYCERATE MUTASE
           (PHOSPHOGLYCEROMUTASE) (BPG-INDEPENDENT PGAM)
           (VEGETATIVE PROTEIN 107) (VEG107)
 pir||D69675 phosphoglycerate mutase (EC 5.4.2.1), 2,
           3-diphosphoglycerate-independent [validated] - Bacillus
           subtilis
 emb|CAB15396.1| (Z99121) phosphoglycerate mutase [Bacillus subtilis]
          Length = 511

 Score =  153 bits (385), Expect = 9e-36
 Identities = 23/144 (15%), Positives = 47/144 (31%), Gaps = 16/144 (11%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVE-RLE 248
             + + L   +++ + D +I +F   D  GH          K +  +D+ +  +V+  L 
Sbjct: 377 YEVKDALVKEIEADKHDAIILNFANPDMVGHSGMVE--PTIKAIEAVDECLGEVVDAILA 434

Query: 249 NDTLLVVAGDHGMTT---NGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQV--S 303
                ++  DHG             +    +    + +                  +   
Sbjct: 435 KGGHAIITADHGNADILITESGEPHTAHTTNPVPVIVTK--------EGITLREGGILGD 486

Query: 304 LVPTLALLLGLPIPFGNIGEVMAE 327
           L PTL  LLG+  P    G  + +
Sbjct: 487 LAPTLLDLLGVEKPKEMTGTSLIQ 510
>pir||T46865 phosphoglycerate mutase (EC 5.4.2.1), 2,
           3-diphosphoglycerate-independent [validated] - Bacillus
           stearothermophilus
 gb|AAD26328.1|AF120091_1 (AF120091) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase [Geobacillus stearothermophilus]
          Length = 511

 Score =  153 bits (385), Expect = 1e-35
 Identities = 24/143 (16%), Positives = 51/143 (34%), Gaps = 16/143 (11%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVE-RLE 248
             + + L   +++ ++D +I ++   D  GH          K +  +D+ +  +V+  L 
Sbjct: 377 YEVTDALLKEIEADKYDAIILNYANPDMVGHSGKLE--PTIKAVEAVDECLGKVVDAILA 434

Query: 249 NDTLLVVAGDHGMTT---NGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQV--S 303
              + ++  DHG        D    +    +    + +                  +   
Sbjct: 435 KGGIAIITADHGNADEVLTPDGKPQTAHTTNPVPVIVTKK--------GIKLRDGGILGD 486

Query: 304 LVPTLALLLGLPIPFGNIGEVMA 326
           L PT+  LLGLP P    G+ + 
Sbjct: 487 LAPTMLDLLGLPQPKEMTGKSLI 509
>pdb|1EJJ|A Chain A, Crystal Structural Analysis Of Phosphoglycerate Mutase
           Cocrystallized With 3-Phosphoglycerate
 pdb|1EQJ|A Chain A, Crystal Structure Of Phosphoglycerate Mutase From Bacillus
           Stearothermophilus Complexed With 2-Phosphoglycerate
          Length = 511

 Score =  153 bits (384), Expect = 1e-35
 Identities = 24/143 (16%), Positives = 51/143 (34%), Gaps = 16/143 (11%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVE-RLE 248
             + + L   +++ ++D +I ++   D  GH          K +  +D+ +  +V+  L 
Sbjct: 377 YEVTDALLKEIEADKYDAIILNYANPDMVGHSGKLE--PTIKAVEAVDECLGKVVDAILA 434

Query: 249 NDTLLVVAGDHGMTT---NGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQV--S 303
              + ++  DHG        D    +    +    + +                  +   
Sbjct: 435 KGGIAIITADHGNADEVLTPDGKPQTAHTTNPVPVIVTKK--------GIKLRDGGILGD 486

Query: 304 LVPTLALLLGLPIPFGNIGEVMA 326
           L PT+  LLGLP P    G+ + 
Sbjct: 487 LAPTMLDLLGLPQPKEMTGKSLI 509
>ref|NP_347349.1| (NC_003030) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase gene [Clostridium acetobutylicum]
 gb|AAK78689.1|AE007586_6 (AE007586) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase gene [Clostridium acetobutylicum]
          Length = 510

 Score =  150 bits (377), Expect = 7e-35
 Identities = 28/143 (19%), Positives = 55/143 (37%), Gaps = 11/143 (7%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVER-LE 248
             + + L   +D  ++D++I +F   D  GH          K +  +D+ +  +V++ L+
Sbjct: 374 YEVTDELLKRLDEDKYDMVILNFANPDMVGHTGILEA--AKKAVETVDECLGKIVDKVLK 431

Query: 249 NDTLLVVAGDHG----MTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSL 304
            D  + +  DHG    M    +    +   V+   F+Y           E         +
Sbjct: 432 LDGSVFITADHGNSEQMIDYSNGKPMTAHTVNPVPFVYVSNHTEAKKLNEGVL----ADI 487

Query: 305 VPTLALLLGLPIPFGNIGEVMAE 327
            PT+   +GL  P    G+ + E
Sbjct: 488 APTMLQEMGLEKPEEMTGKSLFE 510
>pir||I40024 phosphoglycerate mutase - Bacillus subtilis
 gb|AAA21680.1| (L29475) phosphoglycerate mutase [Bacillus subtilis]
          Length = 511

 Score =  150 bits (376), Expect = 1e-34
 Identities = 21/144 (14%), Positives = 46/144 (31%), Gaps = 16/144 (11%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERL-E 248
             + + L   +++ + D +I +F   D  GH          K +  +D+ +  +V+ +  
Sbjct: 377 YEVKDALVKEIEADKHDAIILNFANPDMVGHSGMVE--PTIKAIEAVDECLGEVVDAMVA 434

Query: 249 NDTLLVVAGDHGMTT---NGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQV--S 303
                ++  D G             +    +    + +                  +   
Sbjct: 435 KGGHAIITADDGNADILITESGEPHTAHTTNPVPVIVTK--------EGITLREGGILGD 486

Query: 304 LVPTLALLLGLPIPFGNIGEVMAE 327
           L PTL  LLG+  P    G  + +
Sbjct: 487 LAPTLLDLLGVEKPKEMTGTSLIQ 510
>ref|NP_471880.1| (NC_003212) highly similar to phosphoglycerate mutase [Listeria
           innocua]
 emb|CAC97777.1| (AL596172) highly similar to phosphoglycerate mutase [Listeria
           innocua]
          Length = 510

 Score =  147 bits (369), Expect = 7e-34
 Identities = 24/142 (16%), Positives = 49/142 (33%), Gaps = 12/142 (8%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVE-RLE 248
             + + L   + + + D +I +F   D  GH          K +  +D+ +  +V+  LE
Sbjct: 376 YEVTDALVEDIKNDKHDAIILNFANPDMVGHSGMLE--PTIKAIEAVDENLGRVVDLILE 433

Query: 249 NDTLLVVAGDHGMTTNGD---HGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLV 305
                ++  DHG +           +         + +           E   +    + 
Sbjct: 434 KGGSAIIFADHGNSETMSTPEGKPHTAHTTVPVPVIVTKK----GVTLREGGRL--ADVA 487

Query: 306 PTLALLLGLPIPFGNIGEVMAE 327
           PT+  LLG+  P    GE + +
Sbjct: 488 PTMLDLLGVKKPAEMTGESLIQ 509
>ref|NP_465979.1| (NC_003210) highly similar to phosphoglycerate mutase [Listeria
           monocytogenes EGD-e]
 emb|CAD00534.1| (AL591983) highly similar to phosphoglycerate mutase [Listeria
           monocytogenes]
          Length = 510

 Score =  147 bits (369), Expect = 8e-34
 Identities = 24/142 (16%), Positives = 49/142 (33%), Gaps = 12/142 (8%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVE-RLE 248
             + + L   + + + D +I +F   D  GH          K +  +D+ +  +V+  LE
Sbjct: 376 YEVTDALVEDIKNDKHDAIILNFANPDMVGHSGMLE--PTIKAIEAVDENLGRVVDLILE 433

Query: 249 NDTLLVVAGDHGMTTNGD---HGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLV 305
                ++  DHG +           +         + +           E   +    + 
Sbjct: 434 KGGSAIIFADHGNSETMSTPEGKPHTAHTTVPVPVIVTKK----GVTLREGGRL--ADVA 487

Query: 306 PTLALLLGLPIPFGNIGEVMAE 327
           PT+  LLG+  P    GE + +
Sbjct: 488 PTMLDLLGVKKPAEMTGESLIQ 509
>ref|NP_371299.1| (NC_002758) 2, 3-diphosphoglycerate-independent phosphoglycerate
           mutase [Staphylococcus aureus subsp. aureus Mu50]
 ref|NP_373985.1| (NC_002745) 2, 3-diphosphoglycerate-independentphosphoglycer ate
           mutase [Staphylococcus aureus subsp. aureus N315]
 dbj|BAB41963.1| (AP003131) 2, 3-diphosphoglycerate-independentphosphoglycer ate
           mutase [Staphylococcus aureus subsp. aureus N315]
 dbj|BAB56937.1| (AP003360) 2, 3-diphosphoglycerate-independent phosphoglycerate
           mutase [Staphylococcus aureus subsp. aureus Mu50]
          Length = 505

 Score =  147 bits (368), Expect = 9e-34
 Identities = 27/141 (19%), Positives = 53/141 (37%), Gaps = 12/141 (8%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVER-LE 248
             + + L   ++ G+ D++I +F   D  GH          K +  +D+ +  +V++ L+
Sbjct: 371 YEVKDALLEELNKGDLDLIILNFANPDMVGHSGMLE--PTIKAIEAVDECLGEVVDKILD 428

Query: 249 NDTLLVVAGDHGMTT---NGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLV 305
            D   ++  DHG +      D    +    +    + +           E   +    L 
Sbjct: 429 MDGYAIITADHGNSDQVLTDDDQPMTTHTTNPVPVIVTK----EGVTLRETGRLG--DLA 482

Query: 306 PTLALLLGLPIPFGNIGEVMA 326
           PTL  LL +  P    GE + 
Sbjct: 483 PTLLDLLNVEQPEDMTGESLI 503
>ref|NP_562217.1| (NC_003366) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase [Clostridium perfringens]
 dbj|BAB81007.1| (AP003190) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase [Clostridium perfringens]
          Length = 512

 Score =  146 bits (367), Expect = 1e-33
 Identities = 25/143 (17%), Positives = 54/143 (37%), Gaps = 10/143 (6%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVER-LE 248
             + + L   +D  ++D++I +F   D  GH          K +  +D+ +  + ++ LE
Sbjct: 374 YEVTDELINRLDQDKYDMIILNFANPDMVGHTGVQEA--AVKAIEAVDECLGKVADKVLE 431

Query: 249 NDTLLVVAGDHG----MTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSL 304
            +  L +  DHG    M         +        FL+        +  +  ++     +
Sbjct: 432 KEGTLFITADHGNAEVMIDYSTGKPMTAHTSDPVPFLWVSKDAEGKSLKDGGKL---ADI 488

Query: 305 VPTLALLLGLPIPFGNIGEVMAE 327
            PT+  ++GL +P    G  +  
Sbjct: 489 APTMLTVMGLEVPSEMTGTCLLN 511
>gb|AAF54657.1| (AE003692) CG6790 gene product [Drosophila melanogaster]
 gb|AAL28155.1| (AY060607) GH02677p [Drosophila melanogaster]
          Length = 858

 Score =  142 bits (356), Expect = 2e-32
 Identities = 60/365 (16%), Positives = 103/365 (27%), Gaps = 75/365 (20%)

Query: 13  VCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASRFSRVVL 72
           V       I +    +    L           P    L  G               R+V+
Sbjct: 9   VLVHLLLLICIMRIFYQSGPLSQLE-------PQKTLLDMGV---------PPAADRLVV 52

Query: 73  VLIDALRFDFAQPQHSHRIL---EIQPHHARLYRSQVDPPTTTMQRLKALTTGSLPTFID 129
            L++ LR D     +    +   +I      +  S+   PT T     AL  G  P    
Sbjct: 53  FLLEGLRADTLFSDNCSGAVYIRDIILRQGLVGISKTSVPTLTRSAEVALFAGFNPMPSI 112

Query: 130 AGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSFNVRDLDTVD 189
                       D +  +                      AF K  F    ++ DL    
Sbjct: 113 L------PTSNFDTIFNR--------------------TLAFDKGAFLRFSHLSDLRR-- 144

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVD-----HCGHKHGPHHPEMAKKLSQMDQVIQGLV 244
                     +      V++ +   V        G +   H     + +    ++I+   
Sbjct: 145 RLTKRACLEKLWYANKLVMLVNLAEVGGASPLDIGFQMKLH--NTQRNIRDAYELIEATF 202

Query: 245 ERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAV-----------FPSTPP 293
              ++ T  +    HG+T  G HGG S+ E  A   L+                  +   
Sbjct: 203 N--DSKTAYLYTSAHGLTYFGSHGGGSDEEREAPFLLWGAGVKHVTENITSDFVLNNGVG 260

Query: 294 EEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQ 353
            +   + Q+ L P ++ L+GLP P  N+  +         +    S        +HLNA 
Sbjct: 261 MQLHKLDQIQLAPLMSALIGLPPPVNNLAILPQGYMKVSREYARKS--------VHLNAL 312

Query: 354 QVSRF 358
           Q+   
Sbjct: 313 QLLTQ 317
>ref|NP_244424.1| (NC_002570) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase [Bacillus halodurans]
 dbj|BAB07276.1| (AP001519) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase [Bacillus halodurans]
          Length = 510

 Score =  139 bits (349), Expect = 1e-31
 Identities = 26/145 (17%), Positives = 50/145 (33%), Gaps = 20/145 (13%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVE-RLE 248
             + + L   +++ + DV+I +F   D  GH          K +  +D+ +  +V+  L 
Sbjct: 377 YEVTDALLNEIEADKHDVIILNFANPDMVGHSGMLE--PTIKAVEAVDECLGKVVDAILA 434

Query: 249 NDTLLVVAGDHGMTT---NGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQ---- 301
                V+  DHG        +    +    +    +            EE   + +    
Sbjct: 435 KGGAAVITADHGNADEVVTLEGKPMTAHTTNKVPVIV----------TEEGLNLREDGIL 484

Query: 302 VSLVPTLALLLGLPIPFGNIGEVMA 326
             L PT+  LLG   P    G+ + 
Sbjct: 485 ADLSPTVLDLLGGKQPAEMTGKTLI 509
>sp|P52832|PMGI_PSESM 2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHOGLYCERATE MUTASE
           (PHOSPHOGLYCEROMUTASE) (BPG-INDEPENDENT PGAM)
 pir||A56142 phosphoglycerate mutase (EC 5.4.2.1), 2,
           3-diphosphoglycerate-independent - Pseudomonas syringae
           pv. tomato
 gb|AAA77677.1| (U12776) phosphoglyceromutase [Pseudomonas syringae]
          Length = 510

 Score =  138 bits (345), Expect = 4e-31
 Identities = 24/141 (17%), Positives = 47/141 (33%), Gaps = 12/141 (8%)

Query: 191 GILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLEN- 249
            + + +   ++   +DV+I ++   D  GH          K +  +D  +  + + LE  
Sbjct: 374 EVTDKIVDAIEHQRYDVIIVNYANGDMVGHSGIMEA--AIKAVEYLDVCVGRITDALEKV 431

Query: 250 DTLLVVAGDHG----MTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLV 305
               ++  DHG    MT +      +        F+Y                     + 
Sbjct: 432 GGEALITADHGNVEQMTDDATGQAHTAHTSEPVPFVYVGKRQLKVREGGVL-----ADVA 486

Query: 306 PTLALLLGLPIPFGNIGEVMA 326
           PT+  LLG+  P    G  + 
Sbjct: 487 PTMLQLLGMEKPQEMTGHSIL 507
>gb|AAD26327.1|AF120090_1 (AF120090) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase [Bacillus megaterium]
          Length = 511

 Score =  128 bits (321), Expect = 3e-28
 Identities = 21/142 (14%), Positives = 48/142 (33%), Gaps = 13/142 (9%)

Query: 190 NGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVE-RLE 248
             + + L   ++  + D ++ +F   D  GH          K +  +D+ +  +V+  L 
Sbjct: 377 YEVTDALLAEIEGDKQDAILLNFANPDMVGHSGMLE--PTVKAIETVDECLGKIVDAILA 434

Query: 249 NDTLLVVAGDHGMTT---NGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLV 305
                ++  DHG        +    +    +    + +           E  ++    L 
Sbjct: 435 KGGTAIITADHGNADEVITLEGNPMTAHTTNPVPVIVTKQ----GLELREDGILG--DLA 488

Query: 306 PTLALL-LGLPIPFGNIGEVMA 326
           PT+  L L +  P    G+ + 
Sbjct: 489 PTMLTLFLDVAQPKEMTGKTLI 510
CPU time:   184.79 user secs.	    2.91 sys. secs	  187.70 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.312    0.158    0.451 

Gapped
Lambda     K      H
   0.270   0.0553    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 511439703
Number of Sequences: 887402
Number of extensions: 23350539
Number of successful extensions: 89834
Number of sequences better than 10.0: 375
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 200
Number of HSP's that attempted gapping in prelim test: 88987
Number of HSP's gapped (non-prelim): 532
length of query: 880
length of database: 277,845,442
effective HSP length: 56
effective length of query: 824
effective length of database: 228,150,930
effective search space: 187996366320
effective search space used: 187996366320
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.1 bits)
S2: 77 (34.2 bits)