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GPI Anchor Biosynthesis Report: NP_064419 (PIG-O family, Mus musculus)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (1101 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Results of PSI-Blast iteration 4

Distribution of 61 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)
Sequences producing significant alignments: (bits) Value
gb|AAF57689.1| (AE003799) CG12263 gene product [Drosophila melan... 1209 0.0
ref|NP_064419.1| (NM_020035) phosphatidylinositol glycan, class ... 1417 0.0
dbj|BAB84890.1| (AK074064) FLJ00135 protein [Homo sapiens] 783 0.0
ref|NP_013069.1| (NC_001144) Glycosylphosphatidylinositol (GPI) ... 608 e-172
pir||T02245 hypothetical protein P1.11659_3 - human >gi|2984587|... 577 e-163
ref|NP_491750.1| (NM_059349) C27A12.9.p [Caenorhabditis elegans]... 563 e-159
dbj|BAA82620.1| (AB030280) phosphatidylinositolglycan class N sh... 482 e-134
gb|AAH21148.1|AAH21148 (BC021148) Similar to phosphatidylinosito... 478 e-133
ref|NP_038812.1| (NM_013784) phosphatidylinositol glycan, class ... 476 e-132
ref|NP_036459.1| (NM_012327) phosphatidylinositol glycan, class ... 463 e-129
dbj|BAA82663.1| (AB030316) phosphatidylinositolglycan class N [M... 438 e-121
gb|AAF59557.2| (AC024812) Hypothetical protein Y54E10BR.1 [Caeno... 438 e-121
ref|NP_012756.1| (NC_001143) Required for GPI anchor synthesis; ... 427 e-118
pir||T40715 hypothetical protein SPBC839.08c - fission yeast (S... 421 e-116
ref|NP_186787.1| (NM_111004) putative phosphatidylinositolglycan... 380 e-103
ref|NP_491097.1| (NM_058696) Y54E10BR.1.p [Caenorhabditis elegans] 370 e-101
dbj|BAB29186.1| (AK014165) data source:MGD, source key:MGI:13516... 360 9e-98
ref|NP_197227.1| (NM_121731) unknown protein [Arabidopsis thalia... 359 1e-97
gb|AAF58853.1| (AE003831) CG2292 gene product [Drosophila melano... 352 2e-95
ref|NP_179839.1| (NM_127819) unknown protein [Arabidopsis thalia... 349 1e-94
ref|NP_116023.1| (NM_032634) hypothetical protein MGC3079 [Homo ... 349 2e-94
ref|XP_056208.1| (XM_056208) hypothetical protein MGC3079 [Homo ... 348 4e-94
pir||T46311 hypothetical protein DKFZp434H0711.1 - human (fragme... 343 9e-93
gb|AAF56048.1| (AE003740) CG4907 gene product [Drosophila melano... 303 8e-81
ref|NP_495820.1| (NM_063419) Yeast YJ10 like [Caenorhabditis ele... 289 2e-76
dbj|BAB29052.1| (AK013913) data source:MGD, source key:MGI:18614... 253 9e-66
gb|AAK52677.1|AF321466_1 (AF321466) putative protein Gpi7p [Yarr... 248 3e-64
ref|NP_281901.1| (NC_002163) hypothetical protein Cj0729 [Campyl... 227 1e-57
gb|AAL81172.1| (AE010216) hypothetical protein [Pyrococcus furio... 212 2e-53
ref|NP_143018.1| (NC_000961) hypothetical protein [Pyrococcus ho... 212 3e-53
ref|NP_126799.1| (NC_000868) PHOSPHONOACETATE HYDROLASE related ... 207 1e-51
ref|NP_251941.1| (NC_002516) hypothetical protein [Pseudomonas a... 202 4e-50
dbj|BAB17014.1| (AB050262) hypothetical protein [Macaca fascicul... 190 1e-46
dbj|BAA87261.1| (AB027957) Hypothetical protein [Schizosaccharom... 185 4e-45
pir||T40657 probable phophodiesterase-nucleotide pyrophosp hatas... 179 2e-43
gb|AAF56711.1| (AE003761) CG13978 gene product [Drosophila melan... 172 3e-41
pdb|1EJJ|A Chain A, Crystal Structural Analysis Of Phosphoglycer... 166 2e-39
ref|NP_471880.1| (NC_003212) highly similar to phosphoglycerate ... 165 2e-39
pir||T46865 phosphoglycerate mutase (EC 5.4.2.1), 2, 3-diphospho... 165 2e-39
ref|NP_391271.1| (NC_000964) phosphoglycerate mutase [Bacillus s... 165 3e-39
ref|NP_465979.1| (NC_003210) highly similar to phosphoglycerate ... 164 9e-39
ref|NP_371299.1| (NC_002758) 2, 3-diphosphoglycerate-independent... 163 2e-38
ref|XP_080427.1| (XM_080427) similar to CG12263 gene product (D.... 162 4e-38
pir||I40024 phosphoglycerate mutase - Bacillus subtilis >gi|4602... 160 9e-38
ref|NP_244424.1| (NC_002570) 2,3-bisphosphoglycerate-independent... 147 1e-33
gb|AAD26327.1|AF120090_1 (AF120090) 2,3-bisphosphoglycerate-inde... 140 1e-31
Alignments
>gb|AAF57689.1| (AE003799) CG12263 gene product [Drosophila melanogaster]
          Length = 1077

 Score = 1209 bits (3095), Expect = 0.0
 Identities = 338/1112 (30%), Positives = 515/1112 (45%), Gaps = 75/1112 (6%)

Query: 14   VLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSC---QELPGPGPLP--------WGS 62
             L  L W+ FL  +G+ LF+ GFLL R+  T  S+C      P    +         +  
Sbjct: 5    YLFVLIWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEIFKD 64

Query: 63   HGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQ 122
                    +  + S+V+++++DAL+++F   + +         +P+  KL  LQ +L+  
Sbjct: 65   VNASSNLCL-PQKSKVIVLVVDALKYEFGLYRANATD-----PLPYENKLVVLQELLQQN 118

Query: 123  PHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRV 182
            P H RL R + DPPTTT+QRLK LTTGSLPTFID GSNFAS  I EDN+I Q+      V
Sbjct: 119  PDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPV 178

Query: 183  VFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGV 242
            VF+GD TW DL+P  F +++ + SF++ DL +VDN IL+HL   L+   W VL+AHFLGV
Sbjct: 179  VFLGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGV 238

Query: 243  DHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEV 302
            DHCGHKHGP H EMA+KL +M++VI+ ++  ++NDT L+V GDHGMT +GDHGGD++ E 
Sbjct: 239  DHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDHGMTASGDHGGDTDDET 298

Query: 303  SAALFLYSPTALF-PSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSD 361
            +A LF YS    F  +    + E++ Q+ LVPTLA +LG+PIP+ N G V    F+   D
Sbjct: 299  NALLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPYSNLGLVN---FNIVPD 355

Query: 362  SSHPHSSALAQVSALHIN--AQQVSRFLHTYSAA-TQDLQVKELHRLQTLFSKASARYQH 418
               PH +   Q   LH    AQQ+ R+   Y+    +   V+++  L+T F   + R Q 
Sbjct: 356  LRVPHLNKF-QTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQT 414

Query: 419  FLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLAS 478
                    E A  +   +    LR     C E W RF   +M+ G            L  
Sbjct: 415  VYN-----EVAFKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVV 469

Query: 479  QLAVAPGFLFRP-------LLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSL 531
              +    F           + LI +A G+     +   S  T  +  +      +A   +
Sbjct: 470  NNSRPADFPHIFKAKEVFYVYLINLAAGVFGYRYFKTFSFKTEEQGVIFFTAISSAV--I 527

Query: 532  LPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFFVVEARATPFLLGSLVF 591
            L F      +  +    A       P  +LL   +A FFS+SF + EA+   +LL + + 
Sbjct: 528  LAFHTLRHWTSIATNWSAVKRFGHMPTRLLLFGSMAVFFSNSFVIQEAKILSYLLAAAIL 587

Query: 592  FLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCP 651
             L  +L      L  +    ++  +S      R +    +   +  +   R A    RC 
Sbjct: 588  LLSHELLRLSARLDFR----TKFKASQFL---RSTALRLILASVLAICLIRFAYTLFRCR 640

Query: 652  EETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVL 711
            EE   C            +        Y   V  LV    + RL+LR  GNL    P VL
Sbjct: 641  EEQGNCSDFVNTGGAGFSLKKPGTGKTYILAVVVLVVYTTLTRLYLRSCGNLTGNLPNVL 700

Query: 712  FVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRP 771
              R+G  +  +    +  LA+ + +   R           +   + GL  + +++L W P
Sbjct: 701  LARYGPTVASICAGGHILLANSSIKHIQRTHI------DAMALVIYGLLLVQIIVLSWAP 754

Query: 772  VTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPIT 831
            +   V            L P S    +    + +VP+I+R M+  ++G  +  ++  P+ 
Sbjct: 755  LMTFV------------LPPRSSHTVTINGNESIVPEIFRKMKRMYEGDDDERRSHIPVV 802

Query: 832  VAAYQLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLL---HLLAAGTPV 888
               Y L +VYS+ ++   + LA  +++L   R S+  ++      +LL    +L   T  
Sbjct: 803  ---YGLATVYSSIVIAFGVFLALVMIVLLEPRASIGLVVCVAVGAILLSVHSILRYRTAT 859

Query: 889  ATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLV 948
            +        + A+  W LLA   F++T HQ   S I W AAFVG   G G S  +   LV
Sbjct: 860  SFESCVQPTFTALVGWFLLAHFCFFATSHQTTLSQIEWRAAFVGRTTGIGQSNLVSGALV 919

Query: 949  GANTFASHLLFAVGCPLLLLWPFLCERQGPKR-RQPLPGSESEARVR----PEEEEELQE 1003
              NTF   + F     LL    F      P   R    G + +A         E      
Sbjct: 920  ILNTFCGPIFFFCMYSLLSTETFSLFALFPNLIRSCRSGGKVDASTSMSDLANEAVGFDM 979

Query: 1004 PLMEVRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFE 1063
               E+ L +  + F     +L  ++  + G +I    LA +I  RHLMVWK+FAP+FI+E
Sbjct: 980  TRGELSLYEYEDVFLGTGFKLATQFFMLQGLKIFCAMLACTIHCRHLMVWKIFAPRFIYE 1039

Query: 1064 AVGFIVSSVGLLLGIALVMRVDVAVSSWFKKL 1095
            A+   VS   L++G  LV+R+   V +  K++
Sbjct: 1040 ALATFVSLPALIVGYLLVLRIHRGVDTLIKRI 1071
>ref|NP_064419.1| (NM_020035) phosphatidylinositol glycan, class O [Mus musculus]
 dbj|BAA96254.1| (AB038560) Pig-o [Mus musculus]
          Length = 1101

 Score = 1417 bits (3629), Expect = 0.0
 Identities = 1101/1101 (100%), Positives = 1101/1101 (100%)

Query: 1    MNILFSPRMRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPW 60
            MNILFSPRMRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPW
Sbjct: 1    MNILFSPRMRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPW 60

Query: 61   GSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILE 120
            GSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILE
Sbjct: 61   GSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILE 120

Query: 121  SQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGR 180
            SQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGR
Sbjct: 121  SQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGR 180

Query: 181  RVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFL 240
            RVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFL
Sbjct: 181  RVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFL 240

Query: 241  GVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSEL 300
            GVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSEL
Sbjct: 241  GVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSEL 300

Query: 301  EVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGS 360
            EVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGS
Sbjct: 301  EVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGS 360

Query: 361  DSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFL 420
            DSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFL
Sbjct: 361  DSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFL 420

Query: 421  RDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQL 480
            RDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQL
Sbjct: 421  RDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQL 480

Query: 481  AVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWV 540
            AVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWV
Sbjct: 481  AVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWV 540

Query: 541  SRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFFVVEARATPFLLGSLVFFLVAQLHWE 600
            SRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFFVVEARATPFLLGSLVFFLVAQLHWE
Sbjct: 541  SRGSKRPLAPLLPVPRPVLILLLIRLATFFSDSFFVVEARATPFLLGSLVFFLVAQLHWE 600

Query: 601  GQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSS 660
            GQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSS
Sbjct: 601  GQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSS 660

Query: 661  PWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLM 720
            PWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLM
Sbjct: 661  PWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLM 720

Query: 721  VLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGA 780
            VLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGA
Sbjct: 721  VLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGA 780

Query: 781  ATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSV 840
            ATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSV
Sbjct: 781  ATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSV 840

Query: 841  YSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQA 900
            YSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQA
Sbjct: 841  YSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQA 900

Query: 901  VSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFA 960
            VSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFA
Sbjct: 901  VSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFA 960

Query: 961  VGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEVRLRDAPNHFNAA 1020
            VGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEVRLRDAPNHFNAA
Sbjct: 961  VGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEVRLRDAPNHFNAA 1020

Query: 1021 LLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIAL 1080
            LLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIAL
Sbjct: 1021 LLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIAL 1080

Query: 1081 VMRVDVAVSSWFKKLVLAQQR 1101
            VMRVDVAVSSWFKKLVLAQQR
Sbjct: 1081 VMRVDVAVSSWFKKLVLAQQR 1101
>dbj|BAB84890.1| (AK074064) FLJ00135 protein [Homo sapiens]
          Length = 597

 Score =  783 bits (2002), Expect = 0.0
 Identities = 477/579 (82%), Positives = 508/579 (87%)

Query: 382 QVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFL 441
           QVSRFLHTYSAATQDLQ KELH+LQ LFSKASA YQ  L+ P+ AEA L T+ AE QQFL
Sbjct: 5   QVSRFLHTYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFL 64

Query: 442 RGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLT 501
           RGARA+CIESWARFSLVRMAGG ALLAA+C +CLLASQ A++PGF F PLLL PVAWGL 
Sbjct: 65  RGARAMCIESWARFSLVRMAGGTALLAASCFICLLASQWAISPGFPFCPLLLTPVAWGLV 124

Query: 502 WTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLIL 561
             I YAG+  T   K+DLV+LGAVAA  S LPFLWKAW   GSKRPLA L P+P PVL+L
Sbjct: 125 GAIAYAGLLGTIELKLDLVLLGAVAAVSSFLPFLWKAWAGWGSKRPLATLFPIPGPVLLL 184

Query: 562 LLIRLATFFSDSFFVVEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTA 621
           LL RLA FFSDSF V EARATPFLLGS +  LV QLHWEGQLLPPK LTM RLG+SA T 
Sbjct: 185 LLFRLAVFFSDSFVVAEARATPFLLGSFILLLVVQLHWEGQLLPPKLLTMPRLGTSATTN 244

Query: 622 PPRHSGAHALWLGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGA 681
           PPRH+GA+AL LGIGLLL TRLAGLFHRCPEETP C SSPWLSPLASMVGGRAKNLWYGA
Sbjct: 245 PPRHNGAYALRLGIGLLLCTRLAGLFHRCPEETPVCHSSPWLSPLASMVGGRAKNLWYGA 304

Query: 682 CVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRL 741
           CV ALVALL  VRLWLRRYGNLKSPEPP+LFVRWG+PLM LGTAAYWALASGA+EAPPRL
Sbjct: 305 CVAALVALLAAVRLWLRRYGNLKSPEPPMLFVRWGLPLMALGTAAYWALASGADEAPPRL 364

Query: 742 RALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRTRTILTPFSGPPTSQAD 801
           R LV+GAS VLPRAV GLAA GL LLLW+PVTVLVKAGA   RTRT+LTPFSGPPTSQAD
Sbjct: 365 RVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLVKAGAGAPRTRTVLTPFSGPPTSQAD 424

Query: 802 LDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHV 861
           LDYVVPQIYRHMQEEF+GRLERTK+QGP+TVAAYQLGSVYSAAMVTAL LLAFPL+LLH 
Sbjct: 425 LDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQLGSVYSAAMVTALTLLAFPLLLLHA 484

Query: 862 ERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVF 921
           ER+SLVFLLLFLQSFLLLHLLAAG PV TPGPFTV WQAVSAW L+ATQTFYSTGHQPVF
Sbjct: 485 ERISLVFLLLFLQSFLLLHLLAAGIPVTTPGPFTVPWQAVSAWALMATQTFYSTGHQPVF 544

Query: 922 SAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFA 960
            AIHWHAAFVGFP+GHGS TWLPALLVGANTFASHLLFA
Sbjct: 545 PAIHWHAAFVGFPEGHGSCTWLPALLVGANTFASHLLFA 583
>ref|NP_013069.1| (NC_001144) Glycosylphosphatidylinositol (GPI) biosynthesis; Gpi13p
           [Saccharomyces cerevisiae]
 pir||S64782 probable membrane protein YLL031c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97480.1| (Z73136) ORF YLL031c [Saccharomyces cerevisiae]
          Length = 1017

 Score =  608 bits (1552), Expect = e-172
 Identities = 209/701 (29%), Positives = 307/701 (42%), Gaps = 97/701 (13%)

Query: 3   ILFSPRMRKVS-----VLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGP 57
           I++  R++K        ++ L ++  L +  IA FT GFLL+R  L N SS  E      
Sbjct: 19  IIYKSRIKKFQKNHKFYIILLVFIAILQFISIAFFTRGFLLSRHVLDNISSQNETSK--- 75

Query: 58  LPWGSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQR 117
                        +P RF++ V+++IDALRFDFA P           ++ +   + SL  
Sbjct: 76  -------------LPPRFNKAVILVIDALRFDFAIPVNE---SHSNYNLNYHNNILSLYD 119

Query: 118 ILESQPHHGRLYRSQ-VDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLN 176
              S      L      DPPTTT+QRLK LTTGSLPTFIDAGSNF    I EDN ++QL+
Sbjct: 120 SFASDKDASSLLLKFIADPPTTTLQRLKGLTTGSLPTFIDAGSNFDGTVIEEDNFLKQLH 179

Query: 177 SAGRRVVFMGDDTWRDLF-PGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGS-WDV 234
            A + V F GDDTW  LF P   + +F   S NV DL TVDNG++++ +  L     WDV
Sbjct: 180 LANKTVKFAGDDTWMALFHPFLSNDSFPLESLNVWDLDTVDNGVMDYFHDHLQQDKEWDV 239

Query: 235 LIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDH 294
           +I H LG+DH GHK+GP H  M +K  Q+DQ I  +++ +++DTLLV+ GDHGM   G+H
Sbjct: 240 MIGHMLGIDHVGHKYGPDHFTMREKQIQVDQFIDWILKSIDDDTLLVILGDHGMDHTGNH 299

Query: 295 GGDSELEVSAALFLYSPTALFPSVPP----------EEPEVIPQVSLVPTLALLLGLPIP 344
           GGDS  E+ + LFLYS       +             +   + Q+ LV +LALL+G PIP
Sbjct: 300 GGDSIDELESTLFLYSKKPDMWRLKETSNYNIDNLGHDYRSVRQIDLVSSLALLMGQPIP 359

Query: 345 FGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHR 404
           F N G  + E+     + S   +SA++Q+                Y   T  +       
Sbjct: 360 FNNLGWPIDEIARNDREWSQFVNSAISQL--------------QLY-KDTMQIHHGNDEI 404

Query: 405 LQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGA 464
           L+ L    S          +  +          Q         C E WA+F    +A G 
Sbjct: 405 LEPLAKNIS-NTPPTSDPEKFVKLGHKYQKVFLQT--------CEELWAKFDYYSIATGI 455

Query: 465 ALLAAACLLCLLASQLAVA-----------PGFLFRPLLLIPVAWGLTWTILYAGVSVTT 513
            LLA + +L +  ++L  +           PG +   L+      G+ +   Y   S   
Sbjct: 456 TLLATSLVLLISITKLIPSIVVNQMVPEFVPGIIIMVLVTNLCFHGIFYV--YQQPSFVD 513

Query: 514 GSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPV-PRPVLILLLIRLATFFSD 572
                L+        G  +    +      + R    L     R  ++ ++I    F S+
Sbjct: 514 QFWGTLLATAIGIIIGCYITIFDRYNFIWIAMRLGETLADYWSRIAVMFMIIHALLFTSN 573

Query: 573 SFFVVEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRL--------GSSAPTAPPR 624
           SF + E R   FLL +          +E   LP +  T + L        G+++   P  
Sbjct: 574 SFTIWEDRIVAFLLSTFGMLT----LYEFVFLPKRQSTTALLTATISEKEGTTSGVNPST 629

Query: 625 HSGAHALWL----------GIGLLLFTRLAGLFHRCPEETP 655
            +  +                 L++FTRLA +   C EE  
Sbjct: 630 ANSNYLPLTRFARLLGGYHSAVLIIFTRLASMITICREEQG 670
 Score = 70.9 bits (172), Expect = 1e-10
 Identities = 18/45 (40%), Positives = 27/45 (60%), Gaps = 2/45 (4%)

Query: 1035 QILA--CALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLG 1077
             IL     +  +  RRHLMVWK+F P+FIF ++  IV+ + +  G
Sbjct: 954  TILCLSSFIWVTHFRRHLMVWKIFCPRFIFASLSLIVTQLVVTFG 998
 Score = 38.9 bits (90), Expect = 0.40
 Identities = 31/139 (22%), Positives = 54/139 (38%), Gaps = 20/139 (14%)

Query: 836 QLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFT 895
              ++Y +     ++ +   ++L +     L + L+  Q   LL +L     +       
Sbjct: 803 GYTNIYGSEFFLLVINVLISILLFNKPLAQLSYFLMCNQ---LLSILEIIDLLKLKENII 859

Query: 896 VLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAF-----VGFPDGHGSSTWLPALLVGA 950
                  A  LL+ Q F++TGHQ    ++ W   F     V FP           + +  
Sbjct: 860 GP----IALGLLSYQHFFTTGHQATIPSVQWDIGFMLSEKVTFP--------FTQIAIIL 907

Query: 951 NTFASHLLFAVGCPLLLLW 969
           NTF  H+L ++   LL LW
Sbjct: 908 NTFGPHILVSLSVALLTLW 926
>pir||T02245 hypothetical protein P1.11659_3 - human
 gb|AAC07985.1| (AC004472) P1.11659_3 [Homo sapiens]
          Length = 880

 Score =  577 bits (1472), Expect = e-163
 Identities = 413/536 (77%), Positives = 432/536 (80%), Gaps = 33/536 (6%)

Query: 9   MRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGA 68
           M+K SVLLFLAWVCFLFYAGIALFTSGFLLTRLELTN SSCQE PGPG LPWGS GKPGA
Sbjct: 1   MQKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGA 60

Query: 69  CWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRL 128
           CWM SRFSRVVLVLIDALRFDFAQPQ SH                   RILE QPHH RL
Sbjct: 61  CWMASRFSRVVLVLIDALRFDFAQPQHSH-------------------RILEIQPHHARL 101

Query: 129 YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDD 188
           YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDN+I+QL SAGRRVVFMGDD
Sbjct: 102 YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDD 161

Query: 189 TWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHK 248
           TW+DLFPGAFS+AFFF SFNVRDL TVDNGILEHLYPT+D G WDVLIAHFLGVDHCGHK
Sbjct: 162 TWKDLFPGAFSKAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHK 221

Query: 249 HGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSAALFL 308
           HGPHHPEMAKKLSQMDQVIQGL+ERLENDTLLVVAGDHGMT NGDHGGDSELEVSAALFL
Sbjct: 222 HGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFL 281

Query: 309 YSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSS 368
           YSPTA+FPS PPEEPEVIPQVSLVPTLALLLGLPIPFGN GEVMAELFSGG D S PHSS
Sbjct: 282 YSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGED-SQPHSS 340

Query: 369 ALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEA 428
           ALAQ SALH+NAQQVSRFLHTYSAATQDLQ KELH+LQ LFSKASA YQ  L+ P+ AEA
Sbjct: 341 ALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGAEA 400

Query: 429 ALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLF 488
            L T+ AE QQFLRGARA+CIESWARFSLVRMAG   L   AC+  L+A   AV      
Sbjct: 401 TLPTVIAELQQFLRGARAMCIESWARFSLVRMAGAKNLWYGACVAALVALLAAVRLWLRR 460

Query: 489 RPLLLIP------VAWGL----TWTILY-AGVSVTTGSKIDLVVLGAVAAAGSLLP 533
              L  P      V WGL      T  Y A  S    +   L VL  V+ A  +LP
Sbjct: 461 YGNLKSPEPPMLFVRWGLPLMALGTAAYWALASGADEAPPRLRVL--VSGASMVLP 514
 Score =  288 bits (730), Expect = 4e-76
 Identities = 195/229 (85%), Positives = 207/229 (90%), Gaps = 2/229 (0%)

Query: 666 LASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPEPPVLFVRWGMPLMVLGTA 725
           L  M G  AKNLWYGACV ALVALL  VRLWLRRYGNLKSPEPP+LFVRWG+PLM LGTA
Sbjct: 429 LVRMAG--AKNLWYGACVAALVALLAAVRLWLRRYGNLKSPEPPMLFVRWGLPLMALGTA 486

Query: 726 AYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWRPVTVLVKAGAATSRT 785
           AYWALASGA+EAPPRLR LV+GAS VLPRAV GLAA GL LLLW+PVTVLVKAGA   RT
Sbjct: 487 AYWALASGADEAPPRLRVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLVKAGAGAPRT 546

Query: 786 RTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSVYSAAM 845
           RT+LTPFSGPPTSQADLDYVVPQIYRHMQEEF+GRLERTK+QGP+TVAAYQLGSVYSAAM
Sbjct: 547 RTVLTPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQLGSVYSAAM 606

Query: 846 VTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPF 894
           VTAL LLAFPL+LLH ER+SLVFLLLFLQSFLLLHLLAAG PV TPG +
Sbjct: 607 VTALTLLAFPLLLLHAERISLVFLLLFLQSFLLLHLLAAGIPVTTPGKY 655
 Score =  226 bits (571), Expect = 2e-57
 Identities = 119/147 (80%), Positives = 130/147 (87%), Gaps = 3/147 (2%)

Query: 890  TPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVG 949
            TP PFTV WQAVSAW L+ATQTFYSTGHQPVF AIHWHAAFVGFP+GHGS TWLPALLVG
Sbjct: 731  TPCPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVG 790

Query: 950  ANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEVR 1009
            ANTFASHLLFAVGCPLLLLWPFLCE QG ++RQ  PG+E++ARVR    EE +EPLME+R
Sbjct: 791  ANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVR---PEEEEEPLMEMR 847

Query: 1010 LRDAPNHFNAALLQLGLKYLFILGAQI 1036
            LRDAP HF AALLQLGLKYLFILG+ +
Sbjct: 848  LRDAPQHFYAALLQLGLKYLFILGSYL 874
>ref|NP_491750.1| (NM_059349) C27A12.9.p [Caenorhabditis elegans]
 gb|AAB93646.1| (AF003137) Hypothetical protein C27A12.9 [Caenorhabditis elegans]
          Length = 905

 Score =  563 bits (1436), Expect = e-159
 Identities = 207/668 (30%), Positives = 322/668 (47%), Gaps = 99/668 (14%)

Query: 12  VSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKP-GACW 70
           + +LL    +    +    +F +GFLL R E+ ++SSC +          +H +P   CW
Sbjct: 5   IRILLVSINILLALF----IFQNGFLLKRQEILSKSSCSD----------AHAQPGQTCW 50

Query: 71  MPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYR 130
           M  ++ RV+L+L+DALR+DF  P   +   +      + G++ +++++++S   +  +  
Sbjct: 51  MKQQYQRVILILVDALRYDFLIPIDENTK-KSKEEWYYRGQMKNIEKLVKS--GNVSIGT 107

Query: 131 SQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIV-EDNVIQQLNSAGRRVVFMGDDT 189
              DPPTTT+QRLKALTTG+LPTFIDAG NF+  A++ ED+ + Q    G+ V  +GDDT
Sbjct: 108 LLADPPTTTLQRLKALTTGTLPTFIDAGDNFSPDAVISEDSFVYQAAQLGKNVTLLGDDT 167

Query: 190 WRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLY------------------PTLDGGS 231
           W  LFP  FS+   + SF++ DL+TVD+ I   ++                    +   +
Sbjct: 168 WLSLFPNQFSKTAAYDSFDINDLNTVDDKIARKIFFGKYYNKMVFCFEGPILQDEMLNSN 227

Query: 232 WDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMN 291
             ++IAHFLGVDHCGHK GP HP MA  L +MD++I   IE +++D       DHGMT  
Sbjct: 228 SSIIIAHFLGVDHCGHKFGPSHPVMADTLRKMDRIIGQTIETMKSD-------DHGMTST 280

Query: 292 GDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEV 351
           GDHGG+SE E+ A + ++S       + PE P  I Q+ +VPT++LL+GLPIPF N G V
Sbjct: 281 GDHGGESENEIRAGILVHSKKHQI--ILPERP--IHQIDIVPTISLLMGLPIPFSNLGTV 336

Query: 352 MAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSK 411
           + +LF             +A+     +    V  F  TY+A        ELH        
Sbjct: 337 ITQLF-------TRDLWEIARQMMGFVWQDSVGIFAETYAAQK---NFGELH-------- 378

Query: 412 ASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAAC 471
                 H +RD    E  L T++            L   SW +F    +  G   L  A 
Sbjct: 379 -----SHTIRDSNTMEEQLDTMSRIQ--------TLLRVSWTQFDDAYINAGIFSLVEAI 425

Query: 472 LLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLG-AVAAAGS 530
           +  +  +++      ++R         GL    L    + + GS   L+++  AV+   S
Sbjct: 426 MFLI-TNEVMSLEWIIYR------TGCGLLQAALLTDKTDSDGSARTLLLMTLAVSCLSS 478

Query: 531 LLPFLWKAWVSRGS-KRPLAPLLPVPRPVLILLLIRLATFFSDSFFVVEARATPFLLGSL 589
           ++    KA   R S K  L+  +  P    IL++I   + FS+S+ V EA+   + L SL
Sbjct: 479 IISMCHKALQIRISIKSMLSAKVIGP----ILVIIHSISLFSNSYVVYEAQVVRYFLQSL 534

Query: 590 VFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLLFTRLAGLFHR 649
           V  +  +      L P KP  ++ L           +    L +   +++  R   +FHR
Sbjct: 535 VTLVFLEKLRSTALRPRKPFKLNFLWKFV-------NQDQNLIIFTLIMVGLRSEPIFHR 587

Query: 650 CPEETPAC 657
           C EE   C
Sbjct: 588 CREEEVGC 595
 Score =  158 bits (398), Expect = 4e-37
 Identities = 54/165 (32%), Positives = 71/165 (42%), Gaps = 44/165 (26%)

Query: 905  VLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAVGCP 964
             LL    FY  GH PV S+I W+AAFVG P G  +   LPAL V       HL F+    
Sbjct: 755  ALLIPLGFYHLGHSPVISSIPWNAAFVGIPGGT-ALRVLPALFVLF-----HLNFSAISS 808

Query: 965  LLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEEELQEPLMEVRLRDAPNHFNAALLQL 1024
            + ++     E +  ++              P     L E L+ + +R             
Sbjct: 809  IFVI---SSESESSQKMS-----------APNTAWTLTETLVLMTIR------------- 841

Query: 1025 GLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIV 1069
                     A +    L +SI RRHLMVWK+FAPKFIFE +  IV
Sbjct: 842  ---------ATLTC--LVSSIHRRHLMVWKIFAPKFIFECILTIV 875
>dbj|BAA82620.1| (AB030280) phosphatidylinositolglycan class N short form [Mus
           musculus]
          Length = 798

 Score =  482 bits (1229), Expect = e-134
 Identities = 85/517 (16%), Positives = 143/517 (27%), Gaps = 96/517 (18%)

Query: 14  VLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPS 73
           +L F   +   F   +++F   F                P    +       P    +P 
Sbjct: 2   LLFFALGLLIHFVFFVSIFDIYF--------------TSPLVHGMT------PQFTPLPP 41

Query: 74  RFSRVVLVLIDALRFDFAQP----QRSHVPGEPPVSVPFLGKLGSLQR-ILESQPHHGRL 128
              R+VL + D LR D          S  P    V +       S  R   ES+P H  L
Sbjct: 42  PAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSWGVSHTRVPTESRPGHVAL 101

Query: 129 YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDD 188
                +  +   +  K         F ++   ++  +     +  +  S        GD 
Sbjct: 102 IAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGAS--------GDH 153

Query: 189 TWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWD------------VLI 236
            +       +S       F   D   +D  + + +    D    +            V  
Sbjct: 154 VYT------YSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFF 207

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLE------NDTLLVVAGDHGMTM 290
            H LGVD  GH H P   E    + ++D  ++ ++   +        T  +   DHGMT 
Sbjct: 208 LHLLGVDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTD 267

Query: 291 NGDHGGDSELEVSAALFLYSPT---ALFPSVPPEEPEV-------------IPQVSLVPT 334
            G HG     E       +          S    + E              + Q  + P 
Sbjct: 268 WGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPL 327

Query: 335 LALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAAT 394
           +A L+G+P P  + G +     +          +     +A+ I  Q   +      A  
Sbjct: 328 MASLIGVPFPLNSVGILPVGYLNNTG----LFKAESMFTNAVQILEQFKVKMTQKKEATL 383

Query: 395 QDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIE--SW 452
             L         T F   S        D Q+ +      +   Q+      +LC E    
Sbjct: 384 PFLF--------TPFKLLS--------DSQQLDILRKARSYIKQEKFDEVVSLCEELIDL 427

Query: 453 ARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFR 489
           A   L         L     + +           L  
Sbjct: 428 ALRGLSYYHT-YDRLFLGINVAVGFVGWMSYTSLLII 463
>gb|AAH21148.1|AAH21148 (BC021148) Similar to phosphatidylinositol glycan, class N [Mus
           musculus]
          Length = 826

 Score =  478 bits (1219), Expect = e-133
 Identities = 84/517 (16%), Positives = 142/517 (27%), Gaps = 96/517 (18%)

Query: 14  VLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPS 73
           +L F   +   F    ++F   F                P    +       P    +P 
Sbjct: 2   LLFFALGLLIHFVFFASIFDIYF--------------TSPLVHGMT------PQFTPLPP 41

Query: 74  RFSRVVLVLIDALRFDFAQP----QRSHVPGEPPVSVPFLGKLGSLQR-ILESQPHHGRL 128
              R+VL + D LR D          S  P    V +       S  R   ES+P H  L
Sbjct: 42  PAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIIHEGSWGVSHTRVPTESRPGHVAL 101

Query: 129 YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDD 188
                +  +   +  K         F ++   ++  +     +  +  S        GD 
Sbjct: 102 IAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGAS--------GDH 153

Query: 189 TWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWD------------VLI 236
            +       +S       F   D   +D  + + +    D    +            V  
Sbjct: 154 VYT------YSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFF 207

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLE------NDTLLVVAGDHGMTM 290
            H LG+D  GH H P   E    + ++D  ++ ++   +        T  +   DHGMT 
Sbjct: 208 LHLLGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTD 267

Query: 291 NGDHGGDSELEVSAALFLYSPT---ALFPSVPPEEPEV-------------IPQVSLVPT 334
            G HG     E       +          S    + E              + Q  + P 
Sbjct: 268 WGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPL 327

Query: 335 LALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAAT 394
           +A L+G+P P  + G +     +          +     +A+ I  Q   +      A  
Sbjct: 328 MASLIGVPFPLNSVGILPVGYLNNTG----LFKAESMFTNAVQILEQFKVKMTQKKEATL 383

Query: 395 QDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIE--SW 452
             L         T F   S        D Q+ +      +   Q+      +LC E    
Sbjct: 384 PFLF--------TPFKLLS--------DSQQLDILRKARSYIKQEKFDEVVSLCEELIDL 427

Query: 453 ARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFR 489
           A   L         L     + +           L  
Sbjct: 428 ALRGLSYYHT-YDRLFLGINVAVGFVGWMSYTSLLII 463
>ref|NP_038812.1| (NM_013784) phosphatidylinositol glycan, class N [Mus musculus]
 dbj|BAA82619.1| (AB030279) phosphatidylinositolglycan class N [Mus musculus]
          Length = 931

 Score =  476 bits (1213), Expect = e-132
 Identities = 85/517 (16%), Positives = 143/517 (27%), Gaps = 96/517 (18%)

Query: 14  VLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPS 73
           +L F   +   F   +++F   F                P    +       P    +P 
Sbjct: 2   LLFFALGLLIHFVFFVSIFDIYF--------------TSPLVHGMT------PQFTPLPP 41

Query: 74  RFSRVVLVLIDALRFDFAQP----QRSHVPGEPPVSVPFLGKLGSLQR-ILESQPHHGRL 128
              R+VL + D LR D          S  P    V +       S  R   ES+P H  L
Sbjct: 42  PAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSWGVSHTRVPTESRPGHVAL 101

Query: 129 YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDD 188
                +  +   +  K         F ++   ++  +     +  +  S        GD 
Sbjct: 102 IAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGAS--------GDH 153

Query: 189 TWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWD------------VLI 236
            +       +S       F   D   +D  + + +    D    +            V  
Sbjct: 154 VYT------YSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFF 207

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLE------NDTLLVVAGDHGMTM 290
            H LGVD  GH H P   E    + ++D  ++ ++   +        T  +   DHGMT 
Sbjct: 208 LHLLGVDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTD 267

Query: 291 NGDHGGDSELEVSAALFLYSPT---ALFPSVPPEEPEV-------------IPQVSLVPT 334
            G HG     E       +          S    + E              + Q  + P 
Sbjct: 268 WGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPL 327

Query: 335 LALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAAT 394
           +A L+G+P P  + G +     +          +     +A+ I  Q   +      A  
Sbjct: 328 MASLIGVPFPLNSVGILPVGYLNNTG----LFKAESMFTNAVQILEQFKVKMTQKKEATL 383

Query: 395 QDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIE--SW 452
             L         T F   S        D Q+ +      +   Q+      +LC E    
Sbjct: 384 PFLF--------TPFKLLS--------DSQQLDILRKARSYIKQEKFDEVVSLCEELIDL 427

Query: 453 ARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFR 489
           A   L         L     + +           L  
Sbjct: 428 ALRGLSYYHT-YDRLFLGINVAVGFVGWMSYTSLLII 463
>ref|NP_036459.1| (NM_012327) phosphatidylinositol glycan, class N; endoplasmic
           reticulum membrane protein [Homo sapiens]
 gb|AAD11432.1| (AF109219) Mcd4p homolog [Homo sapiens]
          Length = 931

 Score =  463 bits (1181), Expect = e-129
 Identities = 76/517 (14%), Positives = 143/517 (26%), Gaps = 96/517 (18%)

Query: 14  VLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPS 73
           +L F   +   F    ++F   F                P    +       P    +P 
Sbjct: 2   LLFFTLGLLIHFVFFASIFDIYF--------------TSPLVHGMT------PQFTPLPP 41

Query: 74  RFSRVVLVLIDALRFDFAQP----QRSHVPGEPPVSVPFLGKLGSLQR-ILESQPHHGRL 128
              R+VL + D LR D          S  P    + +       S  R   ES+P H  L
Sbjct: 42  PARRLVLFVADGLRADALYELDENGNSRAPFIRNIIMHEGSWGISHTRVPTESRPGHVAL 101

Query: 129 YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDD 188
                +  +   +  K         F ++   ++  +     +  +  S        GD 
Sbjct: 102 IAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGAS--------GDH 153

Query: 189 TWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWD------------VLI 236
            +       +S       F  +D   +D  + +++         +            V  
Sbjct: 154 VYT------YSYDAKREDFGAQDATKLDTWVFDNVKDFFHHARNNQSLFSKINEEKIVFF 207

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERL------ENDTLLVVAGDHGMTM 290
            H LG+D  GH H P   +    + ++D  ++ ++         +  T  +   DHGMT 
Sbjct: 208 LHLLGIDTNGHAHRPSSRDYKHNIKKVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTD 267

Query: 291 NGDHGGDSELEVSAALFLYSP---------TALFPSVPPEEPEV-------IPQVSLVPT 334
            G HG     E    L  +              F     +E  +       + Q  + P 
Sbjct: 268 WGSHGAGHPSETLTPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWKRLDVNQADIAPL 327

Query: 335 LALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAAT 394
           +  L+G+P P  + G +  +  +          +     +A+ I  Q   +         
Sbjct: 328 MTSLIGVPFPLNSVGILPVDYLNNT----DLFKAESMFTNAVQILEQFKVKMTQKKEVTL 383

Query: 395 QDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWAR 454
             L         T F   S        D ++        +    +      +LC E    
Sbjct: 384 PFLF--------TPFKLLS--------DSKQFNILRKARSYIKHRKFDEVVSLCKE-LIH 426

Query: 455 FSLVRMAGGAAL--LAAACLLCLLASQLAVAPGFLFR 489
            +L  ++             + +           L  
Sbjct: 427 LALKGLSYYHTYDRFFLGVNVVIGFVGWISYASLLII 463
>dbj|BAA82663.1| (AB030316) phosphatidylinositolglycan class N [Mus musculus]
          Length = 391

 Score =  438 bits (1116), Expect = e-121
 Identities = 71/424 (16%), Positives = 122/424 (28%), Gaps = 77/424 (18%)

Query: 14  VLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPS 73
           +L F   +   F    ++F   F                P    +       P    +P 
Sbjct: 2   LLFFALGLLIHFVFFASIFDIYF--------------TSPLVHGMT------PQFTPLPP 41

Query: 74  RFSRVVLVLIDALRFDFAQP----QRSHVPGEPPVSVPFLGKLGSLQR-ILESQPHHGRL 128
              R+VL + D LR D          S  P    V +       S  R   ES+P H  L
Sbjct: 42  PAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSWGVSHTRVPTESRPGHVAL 101

Query: 129 YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDD 188
                +  +   +  K         F ++   ++  +     +  +  S        GD 
Sbjct: 102 IAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGAS--------GDH 153

Query: 189 TWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWD------------VLI 236
            +       +S       F   D   +D  + + +    D    +            V  
Sbjct: 154 VYT------YSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFF 207

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLE------NDTLLVVAGDHGMTM 290
            H LG+D  GH H P   E    + ++D  ++ ++   +        T  +   DHGMT 
Sbjct: 208 LHLLGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTD 267

Query: 291 NGDHGGDSELEVSAALFLYSPT---ALFPSVPPEEPEV-------------IPQVSLVPT 334
            G HG     E       +          S    + E              + Q  + P 
Sbjct: 268 WGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPL 327

Query: 335 LALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAAT 394
           +A L+G+P P  + G +     +          +     +A+ I  Q   +      A  
Sbjct: 328 MASLIGVPFPLNSVGILPVGYLNNTG----LFKAESMFTNAVQILEQFKVKMTQKKEATL 383

Query: 395 QDLQ 398
             L 
Sbjct: 384 PFLF 387
>gb|AAF59557.2| (AC024812) Hypothetical protein Y54E10BR.1 [Caenorhabditis elegans]
          Length = 929

 Score =  438 bits (1116), Expect = e-121
 Identities = 82/523 (15%), Positives = 163/523 (30%), Gaps = 88/523 (16%)

Query: 13  SVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMP 72
           S    +A +        ++F   +                P    +P             
Sbjct: 2   SWKFLIASITVHLVLIYSIFDVYY--------------TSPLVHGIPPQFINSQ-----E 42

Query: 73  SRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQR------ILESQPHHG 126
           +   R+ ++  D LR+D      +  P + P     + +   +          ES+P H 
Sbjct: 43  APAKRIFIISADGLRYDTF----NKYPDKSPYLHSIMNERKGIYGLSRSHIPTESRPGHV 98

Query: 127 RLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMG 186
            ++    +  +   +  K         F  +  ++   +    N+   L +A        
Sbjct: 99  AIFAGITEDISAVAKGWKKNPVQFDSVFNRSSYSWMWGSPDIVNLFDDLPNA-------- 150

Query: 187 DDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWD------------V 234
                     +FS +     F  +D   +D  + EH    L+    D            +
Sbjct: 151 ---------ESFSYSADEEDFASKDASNLDKWVFEHFENFLETAKTDEALNDKMREQKSI 201

Query: 235 LIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQG---LIERLEND--TLLVVAGDHGMT 289
              H LG+D  GH + P   +    +  +D  I+    L++    D  T  +   DHGMT
Sbjct: 202 FFLHLLGIDTNGHGNKPMSRQYIDNIKVVDSGIEKVQHLVDAFFGDHKTAWLFTSDHGMT 261

Query: 290 MNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTG 349
             G HG  S+ EV      +    +    P  +   + Q+ L P ++ L+G PIP  + G
Sbjct: 262 DWGSHGAGSDDEVLTPFVAWGAG-VKQGGPKLD---LNQIDLAPLISALIGCPIPVNSMG 317

Query: 350 EVMAELFSGGSDSSHPH--SSALAQVSALHI---NAQQVSRFLHTYSAATQDLQVKELHR 404
            +  ++ +    S       +   Q+    I   NA+    +   +         K +  
Sbjct: 318 ILPVQMMNSKGSSYEFKAIEANFKQLKEQIIFLKNAKSRRLWFRQFEK----FGDKAMES 373

Query: 405 LQTLFSKASARYQHFLRDPQEAEAAL--STLTAEFQQFLRGARALCIESWARFSLVRMAG 462
           L+T  ++     +  +     A+ A         + ++ R      +             
Sbjct: 374 LRTTLAQLGRDRRFSVATSLFADNAHLMKEAIVFYHRYDRQMLGAAVS----------CS 423

Query: 463 GAALLAAACLLCLLASQLAVAPGFLFRPLLLIPVAWGLTWTIL 505
             A +A        ++        +   L +IP+A  L +T  
Sbjct: 424 FVAWIALVVSFLHNSTSKENLSLLVPHHLFIIPLACSLLFTAY 466
>ref|NP_012756.1| (NC_001143) Required for GPI anchor synthesis; Mcd4p [Saccharomyces
           cerevisiae]
 sp|P36051|YKQ5_YEAST HYPOTHETICAL 105.7 KD PROTEIN IN TPK3-PIR1 INTERGENIC REGION
 pir||S37786 hypothetical protein YKL165c - yeast (Saccharomyces cerevisiae)
 emb|CAA81489.1| (Z26877) unknown [Saccharomyces cerevisiae]
 emb|CAA82007.1| (Z28165) ORF YKL165c [Saccharomyces cerevisiae]
 prf||2118404A ORF [Saccharomyces cerevisiae]
          Length = 919

 Score =  427 bits (1088), Expect = e-118
 Identities = 82/548 (14%), Positives = 154/548 (27%), Gaps = 105/548 (19%)

Query: 11  KVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACW 70
           K    L    V F  +   ++F   F+               P    +       P    
Sbjct: 4   KTRTTLLAVGVLFHLFYLWSIFDIYFI--------------SPLVHGM------SPYQST 43

Query: 71  MPSRFSRVVLVLIDALRFDFAQPQRSH---------VPGEPPVSVPFLGKLGSLQR-ILE 120
                 R+ L++ D LR D    + +H          P    + +       S  R   E
Sbjct: 44  PTPPAKRLFLIVGDGLRADTTFDKVTHPVSGKTEFLAPFIRSLVMNNATYGISHTRMPTE 103

Query: 121 SQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGR 180
           S+P H  +     +  +   +  K+        F  +   ++  +     + +   S   
Sbjct: 104 SRPGHVAMIAGFYEDVSAVTKGWKSNPVNFDSFFNQSTHTYSFGSPDILPMFKDGASDPN 163

Query: 181 RVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGG---------- 230
           +V     DTW             F  F    +  +D  +  HL                 
Sbjct: 164 KV-----DTW--------MYDHTFEDFTQSSI-ELDAFVFRHLDQLFHNSTLNSTLDYEI 209

Query: 231 --SWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERL-----ENDTLLVVA 283
               +V   H LG D  GH + P+  E    +  +D  I  LI+++     ++ T  +  
Sbjct: 210 RQDGNVFFLHLLGCDTAGHSYRPYSAEYYDNVKYIDDQIPILIDKVNKFFADDKTAFIFT 269

Query: 284 GDHGMTMNGDHGGDSELEVSAALFLYSP---TALFPSVPPEEPEV------------IPQ 328
            DHGM+  G HG          L  +       +    P  +               + Q
Sbjct: 270 ADHGMSAFGSHGDGHPNNTRTPLVAWGAGLNKPVHNPFPVSDNYTENWELSSIKRNDVKQ 329

Query: 329 VSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLH 388
             +   ++ L+G+  P  + GE+      G          A    +A  I  Q + +   
Sbjct: 330 ADIASLMSYLIGVNYPKNSVGELPIAYIDGKES----DKLAALYNNARSILEQYLVKQDE 385

Query: 389 TYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALC 448
              +       KE       F      + H+L +       L    +E + +L       
Sbjct: 386 VIDSQ---FFYKE------YFKFVEKSHSHYLEEI----ETLIQRISEGENYLEQEAITL 432

Query: 449 IESWARFSLVRMA--GGAALLAAACLLCLLASQLAVAPGFLF--RPLLLI--------PV 496
            E   + +L  +             ++             +F    +L          P+
Sbjct: 433 TEELMQITLEGLHYLTTYNWRFIRTIVTFGFVGWIFFSFIIFLKSFILENVIDDQKASPL 492

Query: 497 AWGLTWTI 504
           +  +  +I
Sbjct: 493 SHAVFGSI 500
>pir||T40715 hypothetical protein SPBC839.08c - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA17011.1| (AL021816) hypothetical protein [Schizosaccharomyces pombe]
 emb|CAB46701.1| (AL096796) hypothetical protein [Schizosaccharomyces pombe]
          Length = 935

 Score =  421 bits (1072), Expect = e-116
 Identities = 94/523 (17%), Positives = 161/523 (29%), Gaps = 92/523 (17%)

Query: 16  LFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRF 75
           L L  + F      ++F   F                     +    HG        +  
Sbjct: 5   LLLLGILFHVVFLKSIFDIYF---------------------VTPLIHGMKQYSAGEAPA 43

Query: 76  SRVVLVLIDALRFDFAQ----------PQRSHVPGEPPVSVPFLGKLGSLQR-ILESQPH 124
            R+ L++ D LR D              Q    P    +         S  R   ES+P 
Sbjct: 44  KRLFLIVGDGLRPDKLLQPHSEKVIGEEQTYAAPFLRSIIQNNGTFGVSHTRVPTESRPG 103

Query: 125 HGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVF 184
           H  L     +  +   +  K         F  +   ++  +     +  +  S   RV  
Sbjct: 104 HVALIAGFYEDVSAVTKGWKKNPVNFDSVFNQSRHTYSFGSEDILPMFSEGASDPSRV-- 161

Query: 185 MGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTL-----DGGSWD------ 233
                        F  +     F+   +  +D  + + L   L     D   WD      
Sbjct: 162 -----------DTFMYSSELEDFSSNGIV-LDEWVFDRLDELLAQSLEDKELWDMLHRDK 209

Query: 234 -VLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTL------LVVAGDH 286
            V   H LG+D  GH   P   E  + +  +D  IQ L++++ N          V   DH
Sbjct: 210 IVFFLHLLGIDTIGHNKHPDSVEYVENIQYIDGKIQELVDKMNNYYNNDGASSWVFTADH 269

Query: 287 GMTMNGDHGGDSELEVSAALFLYSPTALFP-----------SVPPEEPEV----IPQVSL 331
           GM+  G HG  +       +  +      P           S+P    E+    I Q  +
Sbjct: 270 GMSDFGSHGDGNLDNTRTPIIAWGAGIQSPTHEKNYGHDEYSLPWNLTEIKRIDIQQADI 329

Query: 332 VPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYS 391
              ++ L+GL  P  + G++  +      D S    + +A ++AL I  Q   +     S
Sbjct: 330 AALMSYLVGLNFPVNSVGQIPLDYL----DCSSRRKAEVALMNALEIGEQYNLKSA---S 382

Query: 392 AATQDLQVKELHRLQTLFSKASARYQHFLRD--PQEAEAALSTLTAEFQQFLRGARALCI 449
                +  +    L+      ++ Y   + D    E E A+       Q  L G R L  
Sbjct: 383 KDQTSIFFRPYSPLRNYTEVQASFYNSVIADIESGEYEIAIEHCFHFSQTVLSGLRYLQR 442

Query: 450 ESWARFSLVRMAGGAALLA-AACLLCLLASQLAVAPGFLFRPL 491
             W    L+R       L+    ++C + S        + +P+
Sbjct: 443 YDWL---LLRSIVFFGYLSWIGYVICFVFSLNIEPSSKIVKPV 482
>ref|NP_186787.1| (NM_111004) putative phosphatidylinositolglycan class N short form
           [Arabidopsis thaliana]
 gb|AAF24617.1|AC010870_10 (AC010870) putative phosphatidylinositolglycan class N short form
           [Arabidopsis thaliana]
          Length = 921

 Score =  380 bits (966), Expect = e-103
 Identities = 82/520 (15%), Positives = 144/520 (26%), Gaps = 114/520 (21%)

Query: 16  LFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRF 75
           L +  V       +++F   F              + P    +       P         
Sbjct: 46  LVVLGVALHAVYMLSIFDIYF--------------KTPIVHGMDP---VPPRFS--EPPA 86

Query: 76  SRVVLVLIDALRFDFA----QPQRSHVPGEPPVSVPFLGKLGSLQR-ILESQPHHGRLYR 130
            R+VL++ D LR D      +  +   P    +         S  R   ES+P H  +  
Sbjct: 87  KRLVLLISDGLRADKFFEPDEEGKYRAPFLRNIIKNQGRWGVSHARPPTESRPGHVAIIA 146

Query: 131 SQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTW 190
              + P+   +  KA        F  +   FA  +     +             +   TW
Sbjct: 147 GFYEDPSAVTKGWKANPVEFDSVFNQSRHTFAFGSPDIIPIFC---------SALPHSTW 197

Query: 191 RDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWD------------VLIAH 238
              +P  +      +SF       +D    +     L+    D            V+  H
Sbjct: 198 NS-YPHEYEDFATDASF-------LDEWSFDQFEGLLNRSHADPKLKELLHQDKLVVFLH 249

Query: 239 FLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLE-----NDTLLVVAGDHGMTMNGD 293
            LG D  GH H P+       +  +D++ + +   LE     N T  +   DHGM+  G 
Sbjct: 250 LLGCDSNGHAHRPYSSIYLNNVKVVDKIAERVYHLLEDYYRDNRTSYIFTADHGMSDKGS 309

Query: 294 HGGDSELEVSAALFLYSPTALFP---------------------SVP-PEEPEV------ 325
           HG          L  +     +P                      +P P +  +      
Sbjct: 310 HGDGHPTNTDTPLVAWGAGIQYPKPASGNSHSDSVTTFVDKHAHDMPTPYDWGLRRVERV 369

Query: 326 -IPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVS 384
            + Q  + P ++ LLGLP P  + G +                      +   I  Q + 
Sbjct: 370 DVNQADIAPLMSTLLGLPCPVNSVGNLPLGYMKLNEAE----EVEAVVANTKQILNQLLR 425

Query: 385 RFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGA 444
           +    Y  ++  L  K    L            H      + +  +S  + E    L   
Sbjct: 426 KS---YIKSSNSLFFKPFKPLV-----------HHSFSLSQIDELISAKSYEAAMKLA-- 469

Query: 445 RALCIESWARFSLVRMAGG--AALLAAACLLCLLASQLAV 482
                      SL  +        L    ++ L  +   +
Sbjct: 470 -----VDLRNLSLEGLHYFQTYDWLMLMTVITLGYTGWMI 504
>ref|NP_491097.1| (NM_058696) Y54E10BR.1.p [Caenorhabditis elegans]
          Length = 348

 Score =  370 bits (940), Expect = e-101
 Identities = 68/391 (17%), Positives = 127/391 (32%), Gaps = 69/391 (17%)

Query: 13  SVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMP 72
           S    +A +        ++F   +                P    +P             
Sbjct: 2   SWKFLIASITVHLVLIYSIFDVYY--------------TSPLVHGIPPQFINSQ-----E 42

Query: 73  SRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQR------ILESQPHHG 126
           +   R+ ++  D LR+D      +  P + P     + +   +          ES+P H 
Sbjct: 43  APAKRIFIISADGLRYDTF----NKYPDKSPYLHSIMNERKGIYGLSRSHIPTESRPGHV 98

Query: 127 RLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMG 186
            ++    +  +   +  K         F  +  ++   +    N+   L +A        
Sbjct: 99  AIFAGITEDISAVAKGWKKNPVQFDSVFNRSSYSWMWGSPDIVNLFDDLPNA-------- 150

Query: 187 DDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWD------------V 234
                     +FS +     F  +D   +D  + EH    L+    D            +
Sbjct: 151 ---------ESFSYSADEEDFASKDASNLDKWVFEHFENFLETAKTDEALNDKMREQKSI 201

Query: 235 LIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQG---LIERLEND--TLLVVAGDHGMT 289
              H LG+D  GH + P   +    +  +D  I+    L++    D  T  +   DHGMT
Sbjct: 202 FFLHLLGIDTNGHGNKPMSRQYIDNIKVVDSGIEKVQHLVDAFFGDHKTAWLFTSDHGMT 261

Query: 290 MNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTG 349
             G HG  S+ EV      +    +    P  +   + Q+ L P ++ L+G PIP  + G
Sbjct: 262 DWGSHGAGSDDEVLTPFVAWGAG-VKQGGPKLD---LNQIDLAPLISALIGCPIPVNSMG 317

Query: 350 EVMAELFSGGSDSSHPH--SSALAQVSALHI 378
            +  ++ +    S       +   QV  L I
Sbjct: 318 ILPVQMMNSKGSSYEFKAIEANFKQVINLEI 348
>dbj|BAB29186.1| (AK014165) data source:MGD, source key:MGI:1351629,
           evidence:ISS~phosphatidylinositol glycan, class
           N~putative [Mus musculus]
          Length = 320

 Score =  360 bits (915), Expect = 9e-98
 Identities = 59/344 (17%), Positives = 100/344 (28%), Gaps = 60/344 (17%)

Query: 14  VLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPS 73
           +L F   +   F    ++F   F                P    +       P    +P 
Sbjct: 2   LLFFALGLLIHFVFFASIFDIYF--------------TSPLVHGMT------PQFTPLPP 41

Query: 74  RFSRVVLVLIDALRFDFAQP----QRSHVPGEPPVSVPFLGKLGSLQR-ILESQPHHGRL 128
              R+VL + D LR D          S  P    V +       S  R   ES+P H  L
Sbjct: 42  PAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSWGVSHTRVPTESRPGHVAL 101

Query: 129 YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDD 188
                +  +   +  K     S   F +    ++  +     +  +  S        GD 
Sbjct: 102 IAGFYEDVSAVAKGWKENPVESDSLFNERKYTWSWGSPDILPMFAKGAS--------GDH 153

Query: 189 TWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWD------------VLI 236
            +       +S       F   D   +D  + + +    D    +            V  
Sbjct: 154 VYT------YSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFF 207

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLE------NDTLLVVAGDHGMTM 290
            H LG+D  GH H P   E    + ++D  ++ ++   +        T  +   DHGMT 
Sbjct: 208 LHLLGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTD 267

Query: 291 NGDHGGDSELEVSAALFLYSPT---ALFPSVPPEEPEVIPQVSL 331
            G HG     E       +          S    + E +   ++
Sbjct: 268 WGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDDEFLKGTNI 311
>ref|NP_197227.1| (NM_121731) unknown protein [Arabidopsis thaliana]
 dbj|BAB10512.1| (AB005238) gene_id:MKP11.10~unknown protein [Arabidopsis thaliana]
          Length = 884

 Score =  359 bits (914), Expect = 1e-97
 Identities = 161/435 (37%), Positives = 220/435 (50%), Gaps = 49/435 (11%)

Query: 91  AQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPPTTTMQRLKALTTGS 150
           A P+ +H         P++ KL  LQ +  +     +++++  DPPTT++QRLK LTTG 
Sbjct: 53  ASPRSNH--DSSSEPKPWMDKLTILQTLAFANDSSAKIFKAFADPPTTSLQRLKGLTTGG 110

Query: 151 LPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVR 210
           LPTFID G++F + AIVEDN I QL   G+R+V MGDDTW  LFP  F +++ F SFNV+
Sbjct: 111 LPTFIDIGNSFGAPAIVEDNFINQLVLNGKRLVMMGDDTWTQLFPNQFQKSYPFPSFNVK 170

Query: 211 DLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGL 270
           DL TVDNG +EHL+PTL    WDVLIAHFLGVDH GH  G     M  KL Q + V++ +
Sbjct: 171 DLDTVDNGCIEHLFPTLFKDDWDVLIAHFLGVDHAGHIFGVDSSPMINKLEQYNSVLEKV 230

Query: 271 IERLEN---------DTLLVVAGDHGMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPE 321
           I  LE+         +T+L+V GDHG T+NGDHGG +  EV   +F  S       VPPE
Sbjct: 231 INILESQAGPGGLHENTMLIVMGDHGQTLNGDHGGGTAEEVETTMFAMSTKKHTTLVPPE 290

Query: 322 -EPEVIPQ--------VSLVPTLALLLGLPIPFGN----TGEVMAELFSGGSDSSHPHSS 368
            +     Q        +S +      L L  PF +     G V  EL++ GS S +   S
Sbjct: 291 FDTSSCKQNKDGKQMCISYIEQQHCQLCLGYPFLSEGAFIGHVNPELYALGSSSWNLDKS 350

Query: 369 ALAQ--------------VSALHINAQQVSRFLHTYS-AATQDLQVKELHRLQTLFSKAS 413
            L                V  L +NA QV R++  YS ++       ++ R+  L+S A 
Sbjct: 351 DLGNFGTQSAANEWMKHYVDVLCVNAWQVKRYIDVYSNSSVVGFSSDDMSRISDLYSAAE 410

Query: 414 ARY----QHFLRD------PQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGG 463
             +    +H L D        +  A L    AE+  F      L    W  F+L  M  G
Sbjct: 411 QNWSNSVKHILMDRNGDDGSTDISALLKEQIAEYLNFFSSVVELARSKWTEFNLNLMITG 470

Query: 464 AALLAAACLLCLLAS 478
             +L  + +L  LA 
Sbjct: 471 FGILVISLILQFLAV 485
 Score = 97.7 bits (241), Expect = 9e-19
 Identities = 23/62 (37%), Positives = 36/62 (57%)

Query: 1021 LLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIAL 1080
            L Q+ + +  I    + A  L  +I RRHLMVW +FAPKF+F+ V  I++ + + L  A 
Sbjct: 823  LFQMYMLFGVISATTVTATILCVTIQRRHLMVWGLFAPKFVFDVVDLILTDLLICLASAY 882

Query: 1081 VM 1082
             +
Sbjct: 883  YL 884
 Score = 38.1 bits (88), Expect = 0.77
 Identities = 17/92 (18%), Positives = 33/92 (35%), Gaps = 4/92 (4%)

Query: 513 TGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLILLLIRLATFFSD 572
           T   ++L++ G      SL+      +    S    + L       L ++ IR  +F S+
Sbjct: 460 TEFNLNLMITGFGILVISLILQFLAVFHGDKSYAVGSWLSTGAAFTLFIVTIRACSFLSN 519

Query: 573 SFFVVEARATPFLLGSLVFFLVAQLHWEGQLL 604
           S+ + E +   FLL +              + 
Sbjct: 520 SYILEEGKVANFLLATTGLI----KLRYSVMR 547
 Score = 35.8 bits (82), Expect = 3.8
 Identities = 36/129 (27%), Positives = 63/129 (47%), Gaps = 11/129 (8%)

Query: 855 PLMLLHVERVSLVFLLLFLQSFLLLHL--LAAGTPVATPGPFTVLWQ-AVSAWVLLATQT 911
           P++LL  ++ S++ L+  +  + ++ L  +   T       F+ L    V  W LL+   
Sbjct: 693 PIILLSGKQGSMLALVYLVAGYCIMRLEGVERRTKSDGQSKFSKLNPLCVVQWSLLSICM 752

Query: 912 FYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAV-GCPLLLLWP 970
           F+++GH   F  + + AAFVGF +         A+L+   TF   ++ +V G PLL+  P
Sbjct: 753 FFASGHWCAFDGLRYGAAFVGFDEFV---LIRQAILLTIETFGFSIILSVFGLPLLI--P 807

Query: 971 FLCERQGPK 979
           F    Q P+
Sbjct: 808 F--HSQTPQ 814
 Score = 35.4 bits (81), Expect = 5.9
 Identities = 14/62 (22%), Positives = 26/62 (41%), Gaps = 1/62 (1%)

Query: 20 WVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVV 79
          ++     A + +FT GFLLTR EL   S+C ++     L         +        ++ 
Sbjct: 15 FLLIHAIAIL-IFTRGFLLTRTELPFHSTCSDVSLSPCLASPRSNHDSSSEPKPWMDKLT 73

Query: 80 LV 81
          ++
Sbjct: 74 IL 75
>gb|AAF58853.1| (AE003831) CG2292 gene product [Drosophila melanogaster]
          Length = 874

 Score =  352 bits (894), Expect = 2e-95
 Identities = 87/483 (18%), Positives = 152/483 (31%), Gaps = 82/483 (16%)

Query: 21  VCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVVL 80
           +        ++F   F                P    L    H       +    +R+VL
Sbjct: 7   LVVHVLLLGSIFVIYF--------------RSPVITGLTPQKHLLYY--GLEPPANRLVL 50

Query: 81  VLIDALRFD-FAQPQRSHVPGEPPVSVPFLGKLGSLQR-ILESQPHHGRLYRSQVDPPTT 138
           ++ D  R D F +    +VP    + +       S  R   E++P H  L     + P+ 
Sbjct: 51  IVTDGFRADSFFEENCRYVPNLRKIFLREGVVGVSRTRVPTETRPGHITLIAGLYEDPSA 110

Query: 139 TMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGR-RVVFMGDDTWRDLFPGA 197
            ++  K+        F  +   +A  A    NV   +++ G   + F   D   D  PG 
Sbjct: 111 VLRGWKSNPIDFDTVFNRSSQTYAWGANDVLNVFSHVSNGGEINLRFYNHD--LDFSPGY 168

Query: 198 FSQAFFFSSFNVRDLHTVDNGILEHL-------YPTLDGGSWDVLIAHFLGVDHCGHKHG 250
                        D +  D  + + +          L      V   H LG+D  GH H 
Sbjct: 169 -------------DAYEEDEWVFKRVKLLLQQKREALQRAQNVVFFLHLLGLDTAGHVHK 215

Query: 251 PHHPEMAKKLSQMDQVIQGLIERLE-----NDTLLVVAGDHGMTMNGDHGGDSELEVSAA 305
           P  P+  + L + ++ +  + +  E       T  ++  DHGMT     G    +     
Sbjct: 216 PGAPKFRRTLEKTEKGVYAIYQEFERVFPDKRTAYLLTADHGMTD---SGASRAVPKPGG 272

Query: 306 LFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGSDSSHP 365
              + P    P++P  E   + Q  L P ++ LLGL  P  N G++     +     S  
Sbjct: 273 R-TFMPNNEGPAMPLHE---LEQAQLTPLMSALLGLAPPMNNFGKLPLGYLN----VSKE 324

Query: 366 HSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFLRDPQE 425
           + +  A V+AL +  Q V+          +      L     L ++A  RY+  +   Q 
Sbjct: 325 YEAMAAHVNALQLLEQYVALQKQ----HKKGFFADILLEYDVLNAEAIKRYKDTIVQLQ- 379

Query: 426 AEAALSTLTAEFQQFLRGARALCIES---WARFSLVRMAGGAAL--LAAACLLCLLASQL 480
                     E+        +LCI+        +L  +                      
Sbjct: 380 -------KKREY--------SLCIDKSQVIMSQALEGIDYYHGYYRRVLLLSTTSTFLGW 424

Query: 481 AVA 483
              
Sbjct: 425 MFY 427
>ref|NP_179839.1| (NM_127819) unknown protein [Arabidopsis thaliana]
 gb|AAF18652.1| (AC006592) unknown protein [Arabidopsis thaliana]
          Length = 897

 Score =  349 bits (888), Expect = 1e-94
 Identities = 115/385 (29%), Positives = 177/385 (45%), Gaps = 26/385 (6%)

Query: 9   MRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGA 68
           M    + +F      L   G+++F  GF   +  L+  S         P    S     +
Sbjct: 6   MTCTRLTIFTVAGILLQIIGLSIFVFGFFPVKPTLSGVSGS--ESYRDPFCDSSLISNES 63

Query: 69  CWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEP-PVSVPFLGKLGSLQRILESQPHHGR 127
                   R++  +ID L  +F   +    P +    S+P+   L +             
Sbjct: 64  ELHHPEKLRLLYQVIDGLPAEFVLGKDGKPPEKVLKESMPYTQSLLAN--------GDAI 115

Query: 128 LYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGD 187
            Y ++  PPT TM RLKA+ +G++  F+D   NF + A+++DN++ Q    G ++V +GD
Sbjct: 116 GYHAKAAPPTVTMPRLKAMVSGAIGGFLDVAFNFNTQALLDDNILGQFFRIGWKMVMLGD 175

Query: 188 DTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGH 247
           +TW  LFPG F +    SSF V+D   VD  +  HL   L+   W++LI H+LG+DH GH
Sbjct: 176 ETWLKLFPGLFMRHDGVSSFFVKDTVQVDRNVSRHLPDELNSDDWNLLILHYLGLDHVGH 235

Query: 248 KHGPHHPEMAKKLSQMDQVIQGLI-------ERLENDTLLVVAGDHGMTMNGDHGGDSEL 300
             G + P M  KL +MD +++ +           +  TLL++  DHGMT NG+HGG S  
Sbjct: 236 TGGRNSPLMPAKLKEMDDIVRTMHLRAMMDRSHDQGQTLLIIVSDHGMTENGNHGGSSYE 295

Query: 301 EVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGS 360
           E  + +      +           V  QV L PTLALL G+PIP  N G ++        
Sbjct: 296 ETDSLMLFIGLNSNISDYASATNNVAFQVDLAPTLALLFGVPIPKNNVGVLVPGTLCSLR 355

Query: 361 DSSHPHSSALAQVSALHINAQQVSR 385
           D          Q+ AL +N+ Q+ R
Sbjct: 356 D--------FEQLRALELNSWQLLR 372
>ref|NP_116023.1| (NM_032634) hypothetical protein MGC3079 [Homo sapiens]
 gb|AAH01030.1|AAH01030 (BC001030) Similar to phosphatidylinositol glycan, class O [Homo
            sapiens]
          Length = 454

 Score =  349 bits (887), Expect = 2e-94
 Identities = 197/230 (85%), Positives = 208/230 (89%), Gaps = 3/230 (1%)

Query: 872  FLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFV 931
            F  SFLLLHLLAAG PV TPGPFTV WQAVSAW L+ATQTFYSTGHQPVF AIHWHAAFV
Sbjct: 228  FSLSFLLLHLLAAGIPVTTPGPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFV 287

Query: 932  GFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEA 991
            GFP+GHGS TWLPALLVGANTFASHLLFAVGCPLLLLWPFLCE QG ++RQ  PG+E++A
Sbjct: 288  GFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADA 347

Query: 992  RVRPEEEEELQEPLMEVRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLM 1051
            RVR    EE +EPLME+RLRDAP HF AALLQLGLKYLFILG QILACALAASILRRHLM
Sbjct: 348  RVR---PEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGIQILACALAASILRRHLM 404

Query: 1052 VWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR 1101
            VWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVD AVSSWF++L LAQQR
Sbjct: 405  VWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDGAVSSWFRQLFLAQQR 454
 Score =  293 bits (743), Expect = 1e-77
 Identities = 196/230 (85%), Positives = 206/230 (89%), Gaps = 1/230 (0%)

Query: 227 LDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDH 286
           +D G W+V   H LGVDHCGHKHGPHHPEMAKKLSQMDQVIQGL+ERLENDTLLVVAGDH
Sbjct: 1   MDSGEWEVSNQHVLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDH 60

Query: 287 GMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFG 346
           GMT NGDHGGDSELEVSAALFLYSPTA+FPS PPEEPEVIPQVSLVPTLALLLGLPIPFG
Sbjct: 61  GMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFG 120

Query: 347 NTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQ 406
           N GEVMAELFSGG D S PHSSALAQ SALH+NAQQVSRFLHTYSAATQDLQ KELH+LQ
Sbjct: 121 NIGEVMAELFSGGED-SQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQ 179

Query: 407 TLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFS 456
            LFSKASA YQ  L+ P+ AEA L T+ AE QQFLRGARA+CIESWARFS
Sbjct: 180 NLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWARFS 229
>ref|XP_056208.1| (XM_056208) hypothetical protein MGC3079 [Homo sapiens]
 gb|AAH13987.1|AAH13987 (BC013987) Similar to hypothetical protein MGC3079 [Homo sapiens]
          Length = 454

 Score =  348 bits (884), Expect = 4e-94
 Identities = 197/230 (85%), Positives = 208/230 (89%), Gaps = 3/230 (1%)

Query: 872  FLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFV 931
            F  SFLLLHLLAAG PV TPGPFTV WQAVSAW L+ATQTFYSTGHQPVF AIHWHAAFV
Sbjct: 228  FSLSFLLLHLLAAGIPVTTPGPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFV 287

Query: 932  GFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEA 991
            GFP+GHGS TWLPALLVGANTFASHLLFAVGCPLLLLWPFLCE QG ++RQ  PG+E++A
Sbjct: 288  GFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADA 347

Query: 992  RVRPEEEEELQEPLMEVRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLM 1051
            RVR    EE +EPLME+RLRDAP HF AALLQLGLKYLFILG QILACALAASILRRHLM
Sbjct: 348  RVR---PEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGIQILACALAASILRRHLM 404

Query: 1052 VWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR 1101
            VWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVD AVSSWF++L LAQQR
Sbjct: 405  VWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDGAVSSWFRQLFLAQQR 454
 Score =  299 bits (760), Expect = 1e-79
 Identities = 201/230 (87%), Positives = 210/230 (90%), Gaps = 1/230 (0%)

Query: 227 LDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDH 286
           +D G WDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGL+ERLENDTLLVVAGDH
Sbjct: 1   MDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDH 60

Query: 287 GMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFG 346
           GMT NGDHGGDSELEVSAALFLYSPTA+FPS PPEEPEVIPQVSLVPTLALLLGLPIPFG
Sbjct: 61  GMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFG 120

Query: 347 NTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQ 406
           N GEVMAELFSGG D S PHSSALAQ SALH+NAQQVSRFLHTYSAATQDLQ KELH+LQ
Sbjct: 121 NIGEVMAELFSGGED-SQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQ 179

Query: 407 TLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFS 456
            LFSKASA YQ  L+ P+ AEA L T+ AE QQFLRGARA+CIESWARFS
Sbjct: 180 NLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWARFS 229
>pir||T46311 hypothetical protein DKFZp434H0711.1 - human (fragment)
 emb|CAB70839.1| (AL137607) hypothetical protein [Homo sapiens]
          Length = 825

 Score =  343 bits (872), Expect = 9e-93
 Identities = 52/392 (13%), Positives = 108/392 (27%), Gaps = 71/392 (18%)

Query: 134 DPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDL 193
           +  +   +  K         F ++   ++  +     +  +  S        GD  +   
Sbjct: 1   EDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGAS--------GDHVYT-- 50

Query: 194 FPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWD------------VLIAHFLG 241
               +S       F  +D   +D  + +++         +            V   H LG
Sbjct: 51  ----YSYDAKREDFGAQDATKLDTWVFDNVKDFFHHARNNQSLFSKINEEKIVFFLHLLG 106

Query: 242 VDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERL------ENDTLLVVAGDHGMTMNGDHG 295
           +D  GH H P   +    + ++D  ++ ++         +  T  +   DHGMT  G HG
Sbjct: 107 IDTNGHAHRPSSRDYKHNIKKVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHG 166

Query: 296 GDSELEVSAALFLYSP---------TALFPSVPPEEPEV-------IPQVSLVPTLALLL 339
                E    L  +              F     +E  +       + Q  + P +  L+
Sbjct: 167 AGHPSETLTPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWKRLDVNQADIAPLMTSLI 226

Query: 340 GLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQV 399
           G+P P  + G +  +  +          +     +A+ I  Q   +           L  
Sbjct: 227 GVPFPLNSVGILPVDYLNNT----DLFKAESMFTNAVQILEQFKVKMTQKKEVTLPFLF- 281

Query: 400 KELHRLQTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVR 459
                  T F   S        D ++        +    +      +LC E     +L  
Sbjct: 282 -------TPFKLLS--------DSKQFNILRKARSYIKHRKFDEVVSLCKE-LIHLALKG 325

Query: 460 MAGGAAL--LAAACLLCLLASQLAVAPGFLFR 489
           ++             + +           L  
Sbjct: 326 LSYYHTYDRFFLGVNVVIGFVGWISYASLLII 357
>gb|AAF56048.1| (AE003740) CG4907 gene product [Drosophila melanogaster]
          Length = 919

 Score =  303 bits (770), Expect = 8e-81
 Identities = 67/442 (15%), Positives = 133/442 (29%), Gaps = 78/442 (17%)

Query: 21  VCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVVL 80
           +        ++ +  F  T L      S                      +     R+V+
Sbjct: 7   LVVHLLLLGSILSIYFQSTILSDLEPLSTLRE----------------LGLEPPADRLVV 50

Query: 81  VLIDALRFDFAQPQ-RSHVPGEPPVSVPFLGKLGSLQR-ILESQPHHGRLYRSQVDPPTT 138
            ++D LR         S VP    + V       S       ++P H  ++    + P  
Sbjct: 51  FVVDGLRAQSVLADHCSAVPDLRELFVEQALVGISRACPPTVTRPGHIAIFGGFNEDPAA 110

Query: 139 TMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAF 198
           T   L    +     F  + +           V   L + G  + F              
Sbjct: 111 T---LTWNPSTFDSVFNRSRNAIGWMQAEVAKVFTHLPTGGAPLRF-------------- 153

Query: 199 SQAFFFSSFNVRDLHTVDNGILEHLYPTL---------DGGSWDVLIAHFLGVDHCGHKH 249
                F+  ++ D   +D    + +   +            +  +   +   +D   H H
Sbjct: 154 ---ETFARSDISDRLRLDQWTFDKVRNFMTNEQNVQPLRDATSVIFFVYLADIDLAEHVH 210

Query: 250 GPHHPEMAKKLSQMDQVIQGLIERLE-----NDTLLVVAGDHGMTMNGDHGGDSELEVSA 304
            P+     +KL+   + I+   E  E     + T  ++  D+G+++   HGG  E  V  
Sbjct: 211 MPNSLNFREKLNYTQRGIRQTYELFESVFNDSRTAYLMTADYGISL---HGGGGERGVET 267

Query: 305 ALFLYSPTALF------------PSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVM 352
              L+                   + P      + Q+ L P ++ L+GLP P  N   + 
Sbjct: 268 PFILWGAGVKRSAPNPGQNFTAGENGPILPLYQLEQIQLAPLMSALIGLPPPMNNMALMP 327

Query: 353 AELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKA 412
               +    +S  +   +  ++A+ + AQ               +    L + ++L S  
Sbjct: 328 LGFLN----TSVQYELQVLHLNAMQLLAQARILIKR----HEDGILYLLLPKFESLGSAE 379

Query: 413 SARYQH---FLRDPQEAEAALS 431
              Y     +L   ++ E AL 
Sbjct: 380 IENYPQIIKYLVAKEQYEEALK 401
>ref|NP_495820.1| (NM_063419) Yeast YJ10 like [Caenorhabditis elegans]
 pir||T21487 hypothetical protein F28C6.4 - Caenorhabditis elegans
 emb|CAA92675.1| (Z68315) Similarity to Yeast hypothetical protein YJ10
           (SW:YJ10_YEAST)~cDNA EST yk160e12.5 comes from this
           gene~cDNA EST yk160e12.3 comes from this gene~cDNA EST
           yk317h4.3 comes from this gene~cDNA EST yk317h4.5 comes
           from this gene [Caenorhabditis ele>
          Length = 795

 Score =  289 bits (734), Expect = 2e-76
 Identities = 93/332 (28%), Positives = 142/332 (42%), Gaps = 35/332 (10%)

Query: 51  ELPGPGPLPWGSHGKPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLG 110
               P        G   +   P    R+V ++IDA R  F    +S +            
Sbjct: 82  TPTPPTEEFGFKDGNQESSSSP----RLVFMVIDAFRLSFLTSSKSPMSF---------- 127

Query: 111 KLGSLQRILESQPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDN 170
                    E      +L+ +    PT T+ R+ A  TG+LP+F    +N A+  +   N
Sbjct: 128 ------TKSEITKKSAKLFDAYARMPTVTLPRITAYLTGTLPSFGTVLTNLATAEMKTAN 181

Query: 171 VIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTL--D 228
            I ++   G++V F GDDTW  L P +F +    +SF V D   VDN +  HL   L   
Sbjct: 182 WISRIQKIGKKVHFFGDDTWIRLLPRSFEKFEGVTSFFVSDYTDVDNNVTRHLDTELSNT 241

Query: 229 GGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDT------LLVV 282
             SWD LI H+LG+DH GH  G    ++ +KL +MD VI  + + L++ T       L+V
Sbjct: 242 NHSWDALILHYLGLDHIGHSLGGSSSKIPEKLKEMDDVIGRIHKYLKSSTSVDQESYLIV 301

Query: 283 AGDHGMTMNGDHGGDSELEVSAALFLY-------SPTALFPSVPPEEPEVIPQVSLVPTL 335
            GDHGMT  G HGG S  E    + ++       +          ++P  I Q+ +  T+
Sbjct: 302 CGDHGMTAAGSHGGASPDETRVPVVIWKFGREEGNKNYGNQDDSLKQPPRIEQIDVSSTI 361

Query: 336 ALLLGLPIPFGNTGEVMAELFSGGSDSSHPHS 367
             +  +PIP  + G  +   FS   +      
Sbjct: 362 FDVFNMPIPIESYGISLINYFSDDEEEYFEKQ 393
 Score = 35.4 bits (81), Expect = 4.7
 Identities = 31/123 (25%), Positives = 46/123 (37%), Gaps = 21/123 (17%)

Query: 841 YSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQA 900
           YS   V A  L   PL+L  V    ++ LL+      L   L  G+              
Sbjct: 634 YSTGSVLAGCL---PLLLAIVPVSQILLLLVIYNMGKLFSKLNLGS-------------- 676

Query: 901 VSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFA 960
              +V L   +F+ TG+    S +   +A+VG          L  L +   TF+  +LF 
Sbjct: 677 -VEFVTLCMSSFFYTGNSNSLSTLSLTSAYVGLSS---YQPILVGLQLMLYTFSGPILFL 732

Query: 961 VGC 963
            GC
Sbjct: 733 CGC 735
>dbj|BAB29052.1| (AK013913) data source:MGD, source key:MGI:1861452,
            evidence:ISS~phosphatidylinositol glycan, class
            O~putative [Mus musculus]
          Length = 171

 Score =  253 bits (642), Expect = 9e-66
 Identities = 170/171 (99%), Positives = 171/171 (99%)

Query: 931  VGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESE 990
            VGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESE
Sbjct: 1    VGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESE 60

Query: 991  ARVRPEEEEELQEPLMEVRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHL 1050
            ARVRPEEEEELQEPLME+RLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHL
Sbjct: 61   ARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHL 120

Query: 1051 MVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR 1101
            MVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR
Sbjct: 121  MVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR 171
>gb|AAK52677.1|AF321466_1 (AF321466) putative protein Gpi7p [Yarrowia lipolytica]
          Length = 860

 Score =  248 bits (629), Expect = 3e-64
 Identities = 132/449 (29%), Positives = 194/449 (42%), Gaps = 67/449 (14%)

Query: 13  SVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMP 72
              L    +  L  A + LF  GFL +R+ L   +  +                      
Sbjct: 5   RWTL-AVTIVVLQLAAVLLFARGFLPSRVLLPGYTESRVST------------------E 45

Query: 73  SRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQ 132
           + F + +++++DA R DFA   +S+ P                             + + 
Sbjct: 46  APFQKAIIMVVDAFRSDFAFSDQSNCPQLHKRINSGG----------------AIPFTAH 89

Query: 133 VDPPTTTMQRLKALTTGSLPTFIDAGSNFA-----SHAIVEDNVIQQLNSAGRRVVFMGD 187
             PPT T+ R+K LTTGS P F+DA  N A     S    +D+ + Q +  GR++   GD
Sbjct: 90  STPPTVTLPRIKGLTTGSTPNFLDAVLNIAESDNSSTLANQDSWLAQASRDGRKIHMFGD 149

Query: 188 DTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGG-SWDVLIAHFLGVDHCG 246
           DTW  LFPG F      +SF V D   VDN +  H+   LD    WDVLI H+LG+DH G
Sbjct: 150 DTWIKLFPGMFDDCEGTASFFVSDYTEVDNNVTRHIDTQLDQKTEWDVLILHYLGLDHIG 209

Query: 247 HKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSAAL 306
           HK GP  P M  K  +MD +   L    ++DT+L++ GDHGM   G+HGG S  E SAA+
Sbjct: 210 HKTGPESPFMPAKQKEMDDIFDKLYNSCDDDTVLILLGDHGMNEVGNHGGSSAGETSAAM 269

Query: 307 FLYSPTA---------LFPSVPPEEPE----VIPQVSLVPTLALLLGLPIPFGNTGEVMA 353
              SP               +P  +       + Q  LVPTL  LLGL  P  N G +++
Sbjct: 270 VFASPKFETAQLTETAETSPLPWTDTYKYHSRMDQTDLVPTLTALLGLNTPKNNLGVLVS 329

Query: 354 ELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKE-LHRLQTLFSKA 412
           ++       S        Q++ L  NA Q+ + L   ++   D   KE      TL S  
Sbjct: 330 QML---GLWSPED-----QLNVLKNNADQMVQILQGQASRESD--AKEVYELYDTLNSNP 379

Query: 413 SARYQHFLRDPQEAEAALSTLTAEFQQFL 441
           S   + +     EA++ L+  ++ +    
Sbjct: 380 S--VKDYYNFLYEAQSYLTHASSNYNTND 406
 Score = 34.6 bits (79), Expect = 9.7
 Identities = 46/213 (21%), Positives = 78/213 (36%), Gaps = 33/213 (15%)

Query: 881  LLAAGTPVATPGPFTVLWQAVSAWVLLATQ-TFYSTGHQPVFSAIHWHAAFVGFPDGHGS 939
            + ++     T   F  L   +S   LL  Q +F+S G+    ++I    A+ G       
Sbjct: 676  IFSSRDIYQTEALFQKLVLVLSVSTLLLEQVSFFSMGNSNSMASIDLSNAYNGV------ 729

Query: 940  STWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKRRQPLPGSESEARVRPEEEE 999
             T      VG  TF S+       PL      L        R  +    +E       + 
Sbjct: 730  -TLYQIEFVGVLTFVSNW----IGPLYWSTAGLSFLLEDHVRNAIFAKIAEK----NNDV 780

Query: 1000 ELQEPLME--VRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFA 1057
            +L   L++  + LR          + L    + I    I    L     R HL +W VF+
Sbjct: 781  KLTTKLVQQALTLRVY--------VVLAFSSVAISAVMITCFFL-----REHLFIWTVFS 827

Query: 1058 PKFIFEAVGFIV--SSVGLLLGIALVMRVDVAV 1088
            PK +++ V  ++  + V ++L    V+ V  +V
Sbjct: 828  PKLLYQFVWTVLQFALVDVILSSIFVVLVYRSV 860
>ref|NP_281901.1| (NC_002163) hypothetical protein Cj0729 [Campylobacter jejuni]
 pir||D81344 hypothetical protein Cj0729 [imported] - Campylobacter jejuni
           (strain NCTC 11168)
 emb|CAB73003.1| (AL139076) hypothetical protein Cj0729 [Campylobacter jejuni]
          Length = 269

 Score =  227 bits (573), Expect = 1e-57
 Identities = 55/279 (19%), Positives = 102/279 (35%), Gaps = 40/279 (14%)

Query: 76  SRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDP 135
           S+ +LV++D L    A     ++      ++   GK  SL+                 + 
Sbjct: 3   SKAILVVLDGLNCKSASINMGYLNALCKENL---GKFYSLEC----------------EL 43

Query: 136 PTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFP 195
           P+ +    + L TG  P      +N  S +  + ++       G +          +L+ 
Sbjct: 44  PSMSRPLYECLLTGVKPVLSGIINNKLSFS-KQTSIFDLCKEQGLKAGGAAYHWVFELYN 102

Query: 196 --------GAFSQAFFFSSFNVRDLHTVDNGILEHLY---PTLDGG-SWDVLIAHFLGVD 243
                       +    +       +  D+ +  HL+     L    + D  + H + +D
Sbjct: 103 KKEFIPSLHRHIEDENLTLPYGHFYYE-DDYLDSHLFADGEHLRNKYNLDFTLIHSMNID 161

Query: 244 HCGHKHGPHHPEMAKKLSQMDQVI-QGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEV 302
             GHK G H  E A K  ++D +I + L   LE    +++  DHGMT    HGG SE E+
Sbjct: 162 DAGHKFGSHSIEYANKTKKVDILISEYLPTWLEQGINVIITSDHGMTEGKSHGGLSEDEI 221

Query: 303 SAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGL 341
               F +     + +   ++ E+   +        +LGL
Sbjct: 222 LVPFFTFGSAFSYENAKIKQDEICGSI------CEILGL 254
>gb|AAL81172.1| (AE010216) hypothetical protein [Pyrococcus furiosus DSM 3638]
          Length = 374

 Score =  212 bits (536), Expect = 2e-53
 Identities = 39/224 (17%), Positives = 76/224 (33%), Gaps = 17/224 (7%)

Query: 77  RVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPP 136
           ++VL+ +D            ++P    ++      +        +   H  +    V+P 
Sbjct: 5   KLVLISLDG----NGVYNFKYMPFLSELAENGEYFIVDSIFPTLTDLVHTTVMTG-VEPR 59

Query: 137 T--TTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLF 194
                        TG    F D    F  H +++  +IQ +    R V       +  + 
Sbjct: 60  IHWVVENGYYDRITGVKVKFYDFKVAFNPHEVIKAPLIQDILRQ-RGVRIASVSGYT-MP 117

Query: 195 PGAFSQAFFFSSFNVRDLH--------TVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCG 246
           P +      +  F   D            D  +L       +    D+L+ HF  +D   
Sbjct: 118 PFSNVDVRIYPPFFSSDELYRKHGRDWKKDLWVLNSAIYLYEECKPDLLLVHFASIDGMQ 177

Query: 247 HKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTM 290
           H +GP   +  K +  +D  ++ L ERL+++   ++  DHG   
Sbjct: 178 HDYGPESKDALKAIETVDSAVRSLWERLKDEYAFIIFADHGQEE 221
>ref|NP_143018.1| (NC_000961) hypothetical protein [Pyrococcus horikoshii]
 pir||C71052 hypothetical protein PH1114 - Pyrococcus horikoshii
 dbj|BAA30213.1| (AP000005) 370aa long hypothetical protein [Pyrococcus horikoshii]
          Length = 370

 Score =  212 bits (535), Expect = 3e-53
 Identities = 40/225 (17%), Positives = 74/225 (32%), Gaps = 17/225 (7%)

Query: 76  SRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDP 135
            ++ L+ +D            ++P    ++               +   H  +    V+P
Sbjct: 2   KKLALISLDG----NGIYNLKYMPFLSELAEKGDIFTVDSIFPTLTDLVHTTVMTG-VEP 56

Query: 136 PT--TTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDL 193
                         +G    F +    F  H +++  +IQ +    R V       +  +
Sbjct: 57  RHHWVVENGYYDRLSGVRVKFYEYDVAFNPHKVIKAPLIQDILRQ-RGVRIASVSGYT-M 114

Query: 194 FPGAFSQAFFFSSFNVRDLH--------TVDNGILEHLYPTLDGGSWDVLIAHFLGVDHC 245
            P +      F  F   D            D  +L       +    D+L+ HF  +D  
Sbjct: 115 PPFSNVDVRIFPPFFSSDELYRRHGRDWKKDVWVLNSALYLYEECKPDLLLVHFASIDGM 174

Query: 246 GHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTM 290
            H HGP   E  K +  +D  I+ L ERL+++   ++  DHG   
Sbjct: 175 QHDHGPESREALKAVETVDSAIRALWERLKDEYAFIIFADHGQEE 219
 Score = 41.3 bits (96), Expect = 0.10
 Identities = 12/62 (19%), Positives = 20/62 (31%), Gaps = 8/62 (12%)

Query: 292 GDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEV 351
           G HG  +E  V   L L++  A                 + PT+    G+       G+ 
Sbjct: 317 GMHGSLNERVVKVPLVLWNLEAKKEEATLY--------DIAPTILKFFGVEKRVEMIGKS 368

Query: 352 MA 353
           + 
Sbjct: 369 LI 370
>ref|NP_126799.1| (NC_000868) PHOSPHONOACETATE HYDROLASE related [Pyrococcus abyssi]
 pir||A75091 phosphonoacetate hydrolase related PAB2429 - Pyrococcus abyssi
           (strain Orsay)
 emb|CAB50030.1| (AJ248286) PHOSPHONOACETATE HYDROLASE related [Pyrococcus abyssi]
          Length = 369

 Score =  207 bits (522), Expect = 1e-51
 Identities = 40/222 (18%), Positives = 76/222 (34%), Gaps = 17/222 (7%)

Query: 77  RVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPP 136
           ++ L+ +D            H+P    ++      +        +   H  +    V+P 
Sbjct: 2   KLALISLDG----NGVYNLKHMPFLSELAEEGSMFIVDSIFPTLTDLVHTSVMTG-VEPR 56

Query: 137 T--TTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLF 194
                        +G    F +    F  H +++  ++Q L    R +       +  + 
Sbjct: 57  IHWVVENGYYDRLSGVRVKFYEYDVAFNPHKVIKAPLVQDLLRQ-RGIRVASVSGYT-MP 114

Query: 195 PGAFSQAFFFSSFNVRDLH--------TVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCG 246
           P + +    F  F   D            D  +L       +    D+L+ HF  +D   
Sbjct: 115 PFSNTDVRIFPPFFSSDELYRTHGRDWKKDVWVLNSALYLYEECKPDLLLVHFASIDGMQ 174

Query: 247 HKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGM 288
           H HGP   E  K +  +D  ++ L ERL+N+   ++  DHG 
Sbjct: 175 HDHGPESSEALKAVETVDSAVRTLWERLKNEYAFIIFADHGQ 216
 Score = 35.4 bits (81), Expect = 5.0
 Identities = 11/62 (17%), Positives = 18/62 (28%), Gaps = 8/62 (12%)

Query: 292 GDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEV 351
           G HG  +E  +   L ++                     + PT+    GL       GE 
Sbjct: 316 GMHGSMNERVMKVPLIIWGLNVRREGASLY--------DIAPTILKFFGLEKRGDMKGEP 367

Query: 352 MA 353
           + 
Sbjct: 368 LI 369
>ref|NP_251941.1| (NC_002516) hypothetical protein [Pseudomonas aeruginosa]
 pir||E83240 hypothetical protein PA3251 [imported] - Pseudomonas aeruginosa
           (strain PAO1)
 gb|AAG06639.1|AE004747_12 (AE004747) hypothetical protein [Pseudomonas aeruginosa]
          Length = 269

 Score =  202 bits (509), Expect = 4e-50
 Identities = 52/276 (18%), Positives = 92/276 (32%), Gaps = 38/276 (13%)

Query: 78  VVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPPT 137
           V+LVL+D L    A+    H+           G+   L+                 + P+
Sbjct: 5   VILVLLDGLNHSVARDCMGHLQALCGA---GRGQSYRLEC----------------ELPS 45

Query: 138 TTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLF--- 194
            +    + + TG  P       N       E ++     +AG            +L+   
Sbjct: 46  LSRPLYECILTGVPPIDSGILHNDVVRLSQEQSLFHYARAAGLSTAAAAYHWVSELYNRA 105

Query: 195 PGAFSQAFFFSS---------FNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHC 245
           P   ++               F   D +  D+ +           + + ++ H + +D  
Sbjct: 106 PFDPARDRHTDDSALPIQRGHFYWSDHY-PDSHLFADAESLRRRHAPNFMLVHSMNIDDA 164

Query: 246 GHKHGPHHPEMAKKLSQMDQVI-QGLIERLENDTLLVVAGDHGMTMNGDHGGDSELEVSA 304
           GH+HG   P       + D ++ + L + L     + +  DHGM  +  HGG  E E   
Sbjct: 165 GHRHGLASPAYRNAARRADALLSEYLHQWLAAGYQVAITADHGMNEDRSHGGILEEEREV 224

Query: 305 ALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLG 340
            LF++     F      +P    Q  L  TL  +LG
Sbjct: 225 PLFVFGDA--FSRDDLAQPL---QTDLCGTLCEILG 255
>dbj|BAB17014.1| (AB050262) hypothetical protein [Macaca fascicularis]
          Length = 333

 Score =  190 bits (478), Expect = 1e-46
 Identities = 69/232 (29%), Positives = 103/232 (43%), Gaps = 33/232 (14%)

Query: 234 VLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLEND-------TLLVVAGDH 286
           +LI H+LG+DH GH  GP+ P +  KLS+MD V+  +   L++         LLV+ GDH
Sbjct: 1   MLILHYLGLDHIGHISGPNSPLIGHKLSEMDSVLMKIHTSLQSKERETPLPNLLVLCGDH 60

Query: 287 GMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFG 346
           GM+  G HG  S  EV+  L L S            P+ + Q  +  TLA+ LGLPIP  
Sbjct: 61  GMSETGSHGASSMEEVNTPLILISSAFERKPGDIRHPKHVQQTDVAATLAIALGLPIPKD 120

Query: 347 NTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHT----YSAATQDLQVKEL 402
           + G ++  +  G             Q+  LH+N  Q+S+ L      Y       Q K  
Sbjct: 121 SVGSLLFPVVEG--------RPMREQLRFLHLNTVQLSKLLQENVPSYEKDPGFEQFKMS 172

Query: 403 HRL--------------QTLFSKASARYQHFLRDPQEAEAALSTLTAEFQQF 440
            RL              + LF+  S   + +L   +    +LST  A++  +
Sbjct: 173 ERLHGNWIRLYLEEKHSEVLFNLGSKVLRQYLAALKTLSLSLSTQVAQYDMY 224
>dbj|BAA87261.1| (AB027957) Hypothetical protein [Schizosaccharomyces pombe]
          Length = 179

 Score =  185 bits (466), Expect = 4e-45
 Identities = 41/177 (23%), Positives = 68/177 (38%), Gaps = 25/177 (14%)

Query: 234 VLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTL------LVVAGDHG 287
           V   H LG+D  GH   P   E  + +  +D  IQ L++++ N          V   DHG
Sbjct: 6   VFFLHLLGIDTIGHNKHPDSVEYVENIQYIDGKIQELVDKMNNYYNNDGASSWVFTADHG 65

Query: 288 MTMNGDHGGDSELEVSAALFLYS-----PTALFP------SVPPEEPEV----IPQVSLV 332
           M+  G HG  +       +  +      PT          S+P    E+    I Q  + 
Sbjct: 66  MSDFGSHGDGNLDNTRTPIIAWGAGIQSPTHEKNYGHDEYSLPWNLTEIKRIDIQQADIA 125

Query: 333 PTLALLLGLPIPFGNTGEVMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHT 389
             ++ L+GL  P  + G++  +      D S    + +A ++AL I  Q   +    
Sbjct: 126 ALMSYLVGLNFPVNSVGQIPLDYL----DCSSRRKAEVALMNALEIGEQYNLKSASK 178
>pir||T40657 probable phophodiesterase-nucleotide pyrophosp hatase precursor -
           fission yeast (Schizosaccharomyces pombe)
 emb|CAA22177.1| (AL034352) putative nucleotide pyrophosphatase precursor
           [Schizosaccharomyces pombe]
          Length = 485

 Score =  179 bits (452), Expect = 2e-43
 Identities = 52/237 (21%), Positives = 84/237 (34%), Gaps = 34/237 (14%)

Query: 76  SRVVLVLIDALRFDFAQ-----------PQRSHVPGEPPVSVPFLGKLGSLQRIL--ESQ 122
           S V+++ +D  R D+              +  HVP   P          +   I+     
Sbjct: 74  STVIVISLDGFRADYLYRGFTPNLLSLAERNVHVPFLIPSFPSI--TFPNHYTIVTGLYP 131

Query: 123 PHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRV 182
             HG +  +  DP T            S+P      + +     +  N   + N+    V
Sbjct: 132 ESHGIVSNNFFDPVTG------KQFVNSMPECNKDPTWWDKGEPIWVN--AERNNVRSAV 183

Query: 183 VFM-GDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLG 241
               G++     +   +S  F F +     L    + ILE L    D     +L+A+   
Sbjct: 184 HMWPGNEVENHGYRPTYSDGFNFDT----TLREKKDRILEWL-DLPDKDRPQLLLAYAPH 238

Query: 242 VDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLEND-----TLLVVAGDHGMTMNGD 293
           VD  GH  GP  PE+   + ++D VI  LIE L+         ++   DHGM    D
Sbjct: 239 VDMVGHAFGPDSPELNIIIQEVDIVIGELIEGLKKRNIDKHVNIIFLSDHGMAPTSD 295
>gb|AAF56711.1| (AE003761) CG13978 gene product [Drosophila melanogaster]
          Length = 475

 Score =  172 bits (432), Expect = 3e-41
 Identities = 32/245 (13%), Positives = 58/245 (23%), Gaps = 50/245 (20%)

Query: 21  VCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACWMPSRFSRVVL 80
           +        A+  + F  T L                             +     R+V+
Sbjct: 7   LVVHILLMGAILNTYFQHTLLPNLVPQKTMRE----------------LGLEPPADRLVV 50

Query: 81  VLIDALR-FDFAQPQRSHVPGEPPVSVPFLGKLGSLQR-ILESQPHHGRLYRSQVDPPTT 138
            + D LR   F     S VP    +         S       ++P H  ++    + P  
Sbjct: 51  FVTDGLRAATFLANNGSDVPDLKDIYRQQGRIGISRTCAPTMTRPGHIAIFAGFHEDPAA 110

Query: 139 TMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAF 198
           ++  L          F  + +              +L+  G  + F              
Sbjct: 111 SLMHLCYNPGDFDTVFNRSRNMIGWAHSYIVGYFVKLSHGGAPLRF-------------- 156

Query: 199 SQAFFFSSFNVRDLHTV---DNGILEHLYPTLDGGSWD---------VLIAHFLGVDHCG 246
                  S+  RDL      D    + +   L               V   +   +D   
Sbjct: 157 ------DSYMERDLPEKLTCDKWAFDKVENFLRNVDNVREWRNYKPAVFFVYLADMDIAA 210

Query: 247 HKHGP 251
           H+  P
Sbjct: 211 HRFKP 215
>pdb|1EJJ|A Chain A, Crystal Structural Analysis Of Phosphoglycerate Mutase
           Cocrystallized With 3-Phosphoglycerate
 pdb|1EQJ|A Chain A, Crystal Structure Of Phosphoglycerate Mutase From Bacillus
           Stearothermophilus Complexed With 2-Phosphoglycerate
          Length = 511

 Score =  166 bits (417), Expect = 2e-39
 Identities = 31/181 (17%), Positives = 63/181 (34%), Gaps = 20/181 (11%)

Query: 177 SAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLI 236
           S GR   F G+D      P    +   +        +     + + L   ++   +D +I
Sbjct: 345 SGGREEEFPGEDRILINSP----KVPTYDLKPEMSAYE----VTDALLKEIEADKYDAII 396

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIE-RLENDTLLVVAGDHGMTMNGDHG 295
            ++   D  GH          K +  +D+ +  +++  L    + ++  DHG        
Sbjct: 397 LNYANPDMVGHSGKLE--PTIKAVEAVDECLGKVVDAILAKGGIAIITADHGNADEV--- 451

Query: 296 GDSELEVSAALFLYSPTALF---PSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVM 352
             +           +P  +      +   +  ++    L PT+  LLGLP P   TG+ +
Sbjct: 452 -LTPDGKPQTAHTTNPVPVIVTKKGIKLRDGGILG--DLAPTMLDLLGLPQPKEMTGKSL 508

Query: 353 A 353
            
Sbjct: 509 I 509
>ref|NP_471880.1| (NC_003212) highly similar to phosphoglycerate mutase [Listeria
           innocua]
 emb|CAC97777.1| (AL596172) highly similar to phosphoglycerate mutase [Listeria
           innocua]
          Length = 510

 Score =  165 bits (416), Expect = 2e-39
 Identities = 31/182 (17%), Positives = 60/182 (32%), Gaps = 20/182 (10%)

Query: 177 SAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLI 236
           + GR   F G++      P    +   +        +     + + L   +     D +I
Sbjct: 344 NGGRNEEFPGENRILINSP----KVETYDLKPEMSAYE----VTDALVEDIKNDKHDAII 395

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIE-RLENDTLLVVAGDHGMTMNGDHG 295
            +F   D  GH          K +  +D+ +  +++  LE     ++  DHG +      
Sbjct: 396 LNFANPDMVGHSGMLE--PTIKAIEAVDENLGRVVDLILEKGGSAIIFADHGNSETMS-- 451

Query: 296 GDSELEVSAALFLYSPTALF---PSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVM 352
             +            P  +      V   E   +    + PT+  LLG+  P   TGE +
Sbjct: 452 --TPEGKPHTAHTTVPVPVIVTKKGVTLREGGRL--ADVAPTMLDLLGVKKPAEMTGESL 507

Query: 353 AE 354
            +
Sbjct: 508 IQ 509
>pir||T46865 phosphoglycerate mutase (EC 5.4.2.1), 2,
           3-diphosphoglycerate-independent [validated] - Bacillus
           stearothermophilus
 gb|AAD26328.1|AF120091_1 (AF120091) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase [Geobacillus stearothermophilus]
          Length = 511

 Score =  165 bits (416), Expect = 2e-39
 Identities = 31/181 (17%), Positives = 63/181 (34%), Gaps = 20/181 (11%)

Query: 177 SAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLI 236
           S GR   F G+D      P    +   +        +     + + L   ++   +D +I
Sbjct: 345 SGGREEKFPGEDRILINSP----KVPTYDLKPEMSAYE----VTDALLKEIEADKYDAII 396

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIE-RLENDTLLVVAGDHGMTMNGDHG 295
            ++   D  GH          K +  +D+ +  +++  L    + ++  DHG        
Sbjct: 397 LNYANPDMVGHSGKLE--PTIKAVEAVDECLGKVVDAILAKGGIAIITADHGNADEV--- 451

Query: 296 GDSELEVSAALFLYSPTALF---PSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVM 352
             +           +P  +      +   +  ++    L PT+  LLGLP P   TG+ +
Sbjct: 452 -LTPDGKPQTAHTTNPVPVIVTKKGIKLRDGGILG--DLAPTMLDLLGLPQPKEMTGKSL 508

Query: 353 A 353
            
Sbjct: 509 I 509
>ref|NP_391271.1| (NC_000964) phosphoglycerate mutase [Bacillus subtilis]
 sp|P39773|PMGI_BACSU 2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHOGLYCERATE MUTASE
           (PHOSPHOGLYCEROMUTASE) (BPG-INDEPENDENT PGAM)
           (VEGETATIVE PROTEIN 107) (VEG107)
 pir||D69675 phosphoglycerate mutase (EC 5.4.2.1), 2,
           3-diphosphoglycerate-independent [validated] - Bacillus
           subtilis
 emb|CAB15396.1| (Z99121) phosphoglycerate mutase [Bacillus subtilis]
          Length = 511

 Score =  165 bits (415), Expect = 3e-39
 Identities = 33/182 (18%), Positives = 61/182 (33%), Gaps = 20/182 (10%)

Query: 177 SAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLI 236
           S GR   F G++      P    +   +        + V     + L   ++    D +I
Sbjct: 345 SGGREAEFPGEERILINSP----KVATYDLKPEMSAYEVK----DALVKEIEADKHDAII 396

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIE-RLENDTLLVVAGDHGMTMNGDHG 295
            +F   D  GH          K +  +D+ +  +++  L      ++  DHG        
Sbjct: 397 LNFANPDMVGHSGMVE--PTIKAIEAVDECLGEVVDAILAKGGHAIITADHGNADIL--- 451

Query: 296 GDSELEVSAALFLYSPTALF---PSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVM 352
             +E          +P  +      +   E  ++    L PTL  LLG+  P   TG  +
Sbjct: 452 -ITESGEPHTAHTTNPVPVIVTKEGITLREGGILG--DLAPTLLDLLGVEKPKEMTGTSL 508

Query: 353 AE 354
            +
Sbjct: 509 IQ 510
>ref|NP_465979.1| (NC_003210) highly similar to phosphoglycerate mutase [Listeria
           monocytogenes EGD-e]
 emb|CAD00534.1| (AL591983) highly similar to phosphoglycerate mutase [Listeria
           monocytogenes]
          Length = 510

 Score =  164 bits (412), Expect = 9e-39
 Identities = 31/182 (17%), Positives = 60/182 (32%), Gaps = 20/182 (10%)

Query: 177 SAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLI 236
           + GR   F G++      P    +   +        +     + + L   +     D +I
Sbjct: 344 NGGRNEEFPGENRILINSP----KVETYDLQPEMSAYE----VTDALVEDIKNDKHDAII 395

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIE-RLENDTLLVVAGDHGMTMNGDHG 295
            +F   D  GH          K +  +D+ +  +++  LE     ++  DHG +      
Sbjct: 396 LNFANPDMVGHSGMLE--PTIKAIEAVDENLGRVVDLILEKGGSAIIFADHGNSETMS-- 451

Query: 296 GDSELEVSAALFLYSPTALF---PSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVM 352
             +            P  +      V   E   +    + PT+  LLG+  P   TGE +
Sbjct: 452 --TPEGKPHTAHTTVPVPVIVTKKGVTLREGGRL--ADVAPTMLDLLGVKKPAEMTGESL 507

Query: 353 AE 354
            +
Sbjct: 508 IQ 509
>ref|NP_371299.1| (NC_002758) 2, 3-diphosphoglycerate-independent phosphoglycerate
           mutase [Staphylococcus aureus subsp. aureus Mu50]
 ref|NP_373985.1| (NC_002745) 2, 3-diphosphoglycerate-independentphosphoglycer ate
           mutase [Staphylococcus aureus subsp. aureus N315]
 dbj|BAB41963.1| (AP003131) 2, 3-diphosphoglycerate-independentphosphoglycer ate
           mutase [Staphylococcus aureus subsp. aureus N315]
 dbj|BAB56937.1| (AP003360) 2, 3-diphosphoglycerate-independent phosphoglycerate
           mutase [Staphylococcus aureus subsp. aureus Mu50]
          Length = 505

 Score =  163 bits (410), Expect = 2e-38
 Identities = 36/188 (19%), Positives = 66/188 (34%), Gaps = 20/188 (10%)

Query: 170 NVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDG 229
             +    S GR   F G+       P    +   +        + V     + L   L+ 
Sbjct: 332 PHVTYFMSGGRNEEFKGERRRLIDSP----KVATYDLKPEMSAYEVK----DALLEELNK 383

Query: 230 GSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIER-LENDTLLVVAGDHGM 288
           G  D++I +F   D  GH          K +  +D+ +  ++++ L+ D   ++  DHG 
Sbjct: 384 GDLDLIILNFANPDMVGHSGMLE--PTIKAIEAVDECLGEVVDKILDMDGYAIITADHGN 441

Query: 289 TMNGDHGGDSELEVSAALFLYSPTALF---PSVPPEEPEVIPQVSLVPTLALLLGLPIPF 345
           +        ++ +        +P  +      V   E   +    L PTL  LL +  P 
Sbjct: 442 SDQV----LTDDDQPMTTHTTNPVPVIVTKEGVTLRETGRLG--DLAPTLLDLLNVEQPE 495

Query: 346 GNTGEVMA 353
             TGE + 
Sbjct: 496 DMTGESLI 503
>ref|XP_080427.1| (XM_080427) similar to CG12263 gene product (D. melanogaster)
            [Drosophila melanogaster]
          Length = 111

 Score =  162 bits (406), Expect = 4e-38
 Identities = 33/101 (32%), Positives = 53/101 (51%)

Query: 995  PEEEEELQEPLMEVRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWK 1054
              E         E+ L +  + F     +L  ++  + G +I    LA +I  RHLMVWK
Sbjct: 5    ANEAVGFDMTRGELSLYEYEDVFLGTGFKLATQFFMLQGLKIFCAMLACTIHCRHLMVWK 64

Query: 1055 VFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKL 1095
            +FAP+FI+EA+   VS   L++G  LV+R+   V +  K++
Sbjct: 65   IFAPRFIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRI 105
>pir||I40024 phosphoglycerate mutase - Bacillus subtilis
 gb|AAA21680.1| (L29475) phosphoglycerate mutase [Bacillus subtilis]
          Length = 511

 Score =  160 bits (403), Expect = 9e-38
 Identities = 31/182 (17%), Positives = 60/182 (32%), Gaps = 20/182 (10%)

Query: 177 SAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLI 236
           S GR   F G++      P    +   +        + V     + L   ++    D +I
Sbjct: 345 SGGREAEFPGEERILINSP----KVATYDLKPEMSAYEVK----DALVKEIEADKHDAII 396

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERL-ENDTLLVVAGDHGMTMNGDHG 295
            +F   D  GH          K +  +D+ +  +++ +       ++  D G        
Sbjct: 397 LNFANPDMVGHSGMVE--PTIKAIEAVDECLGEVVDAMVAKGGHAIITADDGNADIL--- 451

Query: 296 GDSELEVSAALFLYSPTALF---PSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVM 352
             +E          +P  +      +   E  ++    L PTL  LLG+  P   TG  +
Sbjct: 452 -ITESGEPHTAHTTNPVPVIVTKEGITLREGGILG--DLAPTLLDLLGVEKPKEMTGTSL 508

Query: 353 AE 354
            +
Sbjct: 509 IQ 510
>ref|NP_244424.1| (NC_002570) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase [Bacillus halodurans]
 dbj|BAB07276.1| (AP001519) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase [Bacillus halodurans]
          Length = 510

 Score =  147 bits (369), Expect = 1e-33
 Identities = 31/181 (17%), Positives = 59/181 (32%), Gaps = 20/181 (11%)

Query: 177 SAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLI 236
           S GR   F G++      P    +   +        +     + + L   ++    DV+I
Sbjct: 345 SGGREEPFPGEERILIDSP----KVATYDLKPEMSAYE----VTDALLNEIEADKHDVII 396

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIE-RLENDTLLVVAGDHGMTMNGDHG 295
            +F   D  GH          K +  +D+ +  +++  L      V+  DHG        
Sbjct: 397 LNFANPDMVGHSGMLE--PTIKAVEAVDECLGKVVDAILAKGGAAVITADHGNADEVV-- 452

Query: 296 GDSELEVSAALFLYSPTALF---PSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVM 352
             +           +   +      +   E  ++    L PT+  LLG   P   TG+ +
Sbjct: 453 --TLEGKPMTAHTTNKVPVIVTEEGLNLREDGIL--ADLSPTVLDLLGGKQPAEMTGKTL 508

Query: 353 A 353
            
Sbjct: 509 I 509
>gb|AAD26327.1|AF120090_1 (AF120090) 2,3-bisphosphoglycerate-independent phosphoglycerate
           mutase [Bacillus megaterium]
          Length = 511

 Score =  140 bits (351), Expect = 1e-31
 Identities = 29/182 (15%), Positives = 59/182 (31%), Gaps = 21/182 (11%)

Query: 177 SAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLI 236
           S GR   F G+       P    +   +        +     + + L   ++G   D ++
Sbjct: 345 SGGREAEFPGETRILIDSP----KVATYDLKPEMSAYE----VTDALLAEIEGDKQDAIL 396

Query: 237 AHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIE-RLENDTLLVVAGDHGMTMNGDHG 295
            +F   D  GH          K +  +D+ +  +++  L      ++  DHG        
Sbjct: 397 LNFANPDMVGHSGMLE--PTVKAIETVDECLGKIVDAILAKGGTAIITADHGNADEV--- 451

Query: 296 GDSELEVSAALFLYSPTALF---PSVPPEEPEVIPQVSLVPTLALL-LGLPIPFGNTGEV 351
             +           +P  +      +   E  ++    L PT+  L L +  P   TG+ 
Sbjct: 452 -ITLEGNPMTAHTTNPVPVIVTKQGLELREDGILG--DLAPTMLTLFLDVAQPKEMTGKT 508

Query: 352 MA 353
           + 
Sbjct: 509 LI 510
CPU time:   243.60 user secs.	    3.14 sys. secs	  246.74 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.314    0.164    0.452 

Gapped
Lambda     K      H
   0.270   0.0574    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 606490188
Number of Sequences: 887402
Number of extensions: 26811071
Number of successful extensions: 140554
Number of sequences better than 10.0: 492
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 352
Number of HSP's that attempted gapping in prelim test: 139494
Number of HSP's gapped (non-prelim): 851
length of query: 1101
length of database: 277,845,442
effective HSP length: 56
effective length of query: 1045
effective length of database: 228,150,930
effective search space: 238417721850
effective search space used: 238417721850
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.7 bits)
S2: 78 (34.5 bits)