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GPI Anchor Biosynthesis Report: AAF57689.1(PIG-O family, Drosophila melanogaster)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (1077 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Results of PSI-Blast iteration 4

Distribution of 59 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)
Sequences producing significant alignments: (bits) Value
gb|AAF57689.1| (AE003799) CG12263 gene product [Drosophila melan... 1365 0.0
ref|NP_064419.1| (NM_020035) phosphatidylinositol glycan, class ... 1154 0.0
dbj|BAB84890.1| (AK074064) FLJ00135 protein [Homo sapiens] 615 e-174
ref|NP_013069.1| (NC_001144) Glycosylphosphatidylinositol (GPI) ... 549 e-155
dbj|BAA82620.1| (AB030280) phosphatidylinositolglycan class N sh... 536 e-151
gb|AAH21148.1|AAH21148 (BC021148) Similar to phosphatidylinosito... 533 e-150
ref|NP_038812.1| (NM_013784) phosphatidylinositol glycan, class ... 528 e-148
ref|NP_491750.1| (NM_059349) C27A12.9.p [Caenorhabditis elegans]... 516 e-145
pir||T40030 hypothetical protein SPBC27B12.06 - fission yeast (... 497 e-139
ref|NP_036459.1| (NM_012327) phosphatidylinositol glycan, class ... 488 e-136
pir||T02245 hypothetical protein P1.11659_3 - human >gi|2984587|... 483 e-135
ref|NP_012756.1| (NC_001143) Required for GPI anchor synthesis; ... 447 e-124
ref|NP_186787.1| (NM_111004) putative phosphatidylinositolglycan... 410 e-113
pir||T46311 hypothetical protein DKFZp434H0711.1 - human (fragme... 388 e-106
gb|AAF58853.1| (AE003831) CG2292 gene product [Drosophila melano... 378 e-103
gb|AAF59557.2| (AC024812) Hypothetical protein Y54E10BR.1 [Caeno... 377 e-103
dbj|BAA82663.1| (AB030316) phosphatidylinositolglycan class N [M... 377 e-103
gb|AAF54657.1| (AE003692) CG6790 gene product [Drosophila melano... 351 3e-95
gb|AAF56048.1| (AE003740) CG4907 gene product [Drosophila melano... 341 3e-92
ref|NP_116023.1| (NM_032634) hypothetical protein MGC3079 [Homo ... 333 8e-90
ref|XP_056208.1| (XM_056208) hypothetical protein MGC3079 [Homo ... 332 2e-89
dbj|BAB29186.1| (AK014165) data source:MGD, source key:MGI:13516... 313 8e-84
ref|NP_491097.1| (NM_058696) Y54E10BR.1.p [Caenorhabditis elegans] 313 1e-83
gb|AAK25979.1|AF360269_1 (AF360269) putative nucleotide pyrophos... 246 1e-63
ref|NP_197227.1| (NM_121731) unknown protein [Arabidopsis thalia... 238 4e-61
sp|Q09782|YA93_SCHPO HYPOTHETICAL 85.7 KD PROTEIN C13G6.03 IN CH... 235 2e-60
ref|NP_194698.1| (NM_119114) nucleotide pyrophosphatase - like p... 235 3e-60
dbj|BAB29052.1| (AK013913) data source:MGD, source key:MGI:18614... 234 6e-60
dbj|BAB20681.1| (AB032413) nucleotide pyrophosphatase-like prote... 232 3e-59
ref|NP_194699.1| (NM_119115) nucleotide pyrophosphatase -like pr... 228 5e-58
ref|NP_179839.1| (NM_127819) unknown protein [Arabidopsis thalia... 220 1e-55
ref|NP_105837.1| (NC_002678) hypothetical protein [Mesorhizobium... 220 1e-55
gb|AAF56711.1| (AE003761) CG13978 gene product [Drosophila melan... 218 4e-55
ref|NP_194697.1| (NM_119113) nucleotide pyrophosphatase - like p... 214 6e-54
ref|XP_082200.1| (XM_082200) CG6790 [Drosophila melanogaster] 212 3e-53
pir||T03293 nucleotide pyrophosphatase homolog - rice >gi|818849... 200 1e-49
ref|NP_281901.1| (NC_002163) hypothetical protein Cj0729 [Campyl... 199 2e-49
ref|NP_251941.1| (NC_002516) hypothetical protein [Pseudomonas a... 186 1e-45
dbj|BAA87261.1| (AB027957) Hypothetical protein [Schizosaccharom... 181 4e-44
pir||T40657 probable phophodiesterase-nucleotide pyrophosp hatas... 181 5e-44
ref|NP_006200.1| (NM_006209) ectonucleotide pyrophosphatase/phos... 180 1e-43
dbj|BAA08260.1| (D45421) phosphodiesterase I alpha [Homo sapiens] 180 1e-43
ref|XP_080427.1| (XM_080427) similar to CG12263 gene product (D.... 178 3e-43
gb|AAH03264.1|AAH03264 (BC003264) ectonucleotide pyrophosphatase... 178 3e-43
pir||T33724 hypothetical protein - Zymomonas mobilis >gi|3089614... 176 2e-42
Alignments
>gb|AAF57689.1| (AE003799) CG12263 gene product [Drosophila melanogaster]
          Length = 1077

 Score = 1365 bits (3496), Expect = 0.0
 Identities = 1077/1077 (100%), Positives = 1077/1077 (100%)

Query: 1    MNLTYLFVLIWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNE 60
            MNLTYLFVLIWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNE
Sbjct: 1    MNLTYLFVLIWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNE 60

Query: 61   IFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPD 120
            IFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPD
Sbjct: 61   IFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPD 120

Query: 121  HARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVF 180
            HARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVF
Sbjct: 121  HARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVF 180

Query: 181  LGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDH 240
            LGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDH
Sbjct: 181  LGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDH 240

Query: 241  CGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDHGMTASGDHGGDTDDETNA 300
            CGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDHGMTASGDHGGDTDDETNA
Sbjct: 241  CGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDHGMTASGDHGGDTDDETNA 300

Query: 301  LLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLRVPH 360
            LLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLRVPH
Sbjct: 301  LLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLRVPH 360

Query: 361  LNKFQTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQTVYNEVA 420
            LNKFQTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQTVYNEVA
Sbjct: 361  LNKFQTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQTVYNEVA 420

Query: 421  FKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVVNNSRPADFPHI 480
            FKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVVNNSRPADFPHI
Sbjct: 421  FKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVVNNSRPADFPHI 480

Query: 481  FKAKEVFYVYLINLAAGVFGYRYFKTFSFKTEEQGVIFFTAISSAVILAFHTLRHWTSIA 540
            FKAKEVFYVYLINLAAGVFGYRYFKTFSFKTEEQGVIFFTAISSAVILAFHTLRHWTSIA
Sbjct: 481  FKAKEVFYVYLINLAAGVFGYRYFKTFSFKTEEQGVIFFTAISSAVILAFHTLRHWTSIA 540

Query: 541  TNWSAVKRFGHMPTRLLLFGSMAVFFSNSFVIQEAKILSYLLAAAILLLSHELLRLSARL 600
            TNWSAVKRFGHMPTRLLLFGSMAVFFSNSFVIQEAKILSYLLAAAILLLSHELLRLSARL
Sbjct: 541  TNWSAVKRFGHMPTRLLLFGSMAVFFSNSFVIQEAKILSYLLAAAILLLSHELLRLSARL 600

Query: 601  DFRTKFKASQFLRSTALRLILASVLAICLIRFAYTLFRCREEQGNCSDFVNTGGAGFSLK 660
            DFRTKFKASQFLRSTALRLILASVLAICLIRFAYTLFRCREEQGNCSDFVNTGGAGFSLK
Sbjct: 601  DFRTKFKASQFLRSTALRLILASVLAICLIRFAYTLFRCREEQGNCSDFVNTGGAGFSLK 660

Query: 661  KPGTGKTYILAVVVLVVYTTLTRLYLRSCGNLTGNLPNVLLARYGPTVASICAGGHILLA 720
            KPGTGKTYILAVVVLVVYTTLTRLYLRSCGNLTGNLPNVLLARYGPTVASICAGGHILLA
Sbjct: 661  KPGTGKTYILAVVVLVVYTTLTRLYLRSCGNLTGNLPNVLLARYGPTVASICAGGHILLA 720

Query: 721  NSSIKHIQRTHIDAMALVIYGLLLVQIIVLSWAPLMTFVLPPRSSHTVTINGNESIVPEI 780
            NSSIKHIQRTHIDAMALVIYGLLLVQIIVLSWAPLMTFVLPPRSSHTVTINGNESIVPEI
Sbjct: 721  NSSIKHIQRTHIDAMALVIYGLLLVQIIVLSWAPLMTFVLPPRSSHTVTINGNESIVPEI 780

Query: 781  FRKMKRMYEGDDDERRSHIPVVYGLATVYSSIVIAFGVFLALVMIVLLEPRASIGLVVCV 840
            FRKMKRMYEGDDDERRSHIPVVYGLATVYSSIVIAFGVFLALVMIVLLEPRASIGLVVCV
Sbjct: 781  FRKMKRMYEGDDDERRSHIPVVYGLATVYSSIVIAFGVFLALVMIVLLEPRASIGLVVCV 840

Query: 841  AVGAILLSVHSILRYRTATSFESCVQPTFTALVGWFLLAHFCFFATSHQTTLSQIEWRAA 900
            AVGAILLSVHSILRYRTATSFESCVQPTFTALVGWFLLAHFCFFATSHQTTLSQIEWRAA
Sbjct: 841  AVGAILLSVHSILRYRTATSFESCVQPTFTALVGWFLLAHFCFFATSHQTTLSQIEWRAA 900

Query: 901  FVGRTTGIGQSNLVSGALVILNTFCGPIFFFCMYSLLSTETFSLFALFPNLIRSCRSGGK 960
            FVGRTTGIGQSNLVSGALVILNTFCGPIFFFCMYSLLSTETFSLFALFPNLIRSCRSGGK
Sbjct: 901  FVGRTTGIGQSNLVSGALVILNTFCGPIFFFCMYSLLSTETFSLFALFPNLIRSCRSGGK 960

Query: 961  VDASTSMSDLANEAVGFDMTRGELSLYEYEDVFLGTGFKLATQFFMLQGLKIFCAMLACT 1020
            VDASTSMSDLANEAVGFDMTRGELSLYEYEDVFLGTGFKLATQFFMLQGLKIFCAMLACT
Sbjct: 961  VDASTSMSDLANEAVGFDMTRGELSLYEYEDVFLGTGFKLATQFFMLQGLKIFCAMLACT 1020

Query: 1021 IHCRHLMVWKIFAPRFIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRINKAKVH 1077
            IHCRHLMVWKIFAPRFIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRINKAKVH
Sbjct: 1021 IHCRHLMVWKIFAPRFIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRINKAKVH 1077
>ref|NP_064419.1| (NM_020035) phosphatidylinositol glycan, class O [Mus musculus]
 dbj|BAA96254.1| (AB038560) Pig-o [Mus musculus]
          Length = 1101

 Score = 1154 bits (2954), Expect = 0.0
 Identities = 339/1116 (30%), Positives = 514/1116 (45%), Gaps = 79/1116 (7%)

Query: 3    LTYLFVLIWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEIF 62
            ++ L  L W+ FL  +G+ LF+ GFLL R+  T  S+C+ L   P      +        
Sbjct: 12   VSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPG-PGPLPWGSHG------ 64

Query: 63   KDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRANA-----TDPLPYENKLVVLQELLQQ 117
                  +     + S+V+++++DAL+++F   + +         +P+  KL  LQ +L+ 
Sbjct: 65   ---KPGACWMPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILES 121

Query: 118  NPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLP 177
             P H RL R + DPPTTT+QRLK LTTGSLPTFID GSNFAS  I EDN+I Q+      
Sbjct: 122  QPHHGRLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRR 181

Query: 178  VVFLGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLG 237
            VVF+GD TW DL+P  F +++ + SF++ DL +VDN IL+HL   L+   W VL+AHFLG
Sbjct: 182  VVFMGDDTWRDLFPGAFSQAFFFSSFNVRDLHTVDNGILEHLYPTLDGGSWDVLIAHFLG 241

Query: 238  VDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDHGMTASGDHGGDTDDE 297
            VDHCGHKHGP H EMA+KL +M++VI+ ++  ++NDT L+V GDHGMT +GDHGGD++ E
Sbjct: 242  VDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLLVVAGDHGMTMNGDHGGDSELE 301

Query: 298  TNALLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPYSNLGLVNFNIV---P 354
             +A LF YS    F  +    + E++ Q+ LVPTLA +LG+PIP+ N G V   +     
Sbjct: 302  VSAALFLYSPTALFP-SVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGEVMAELFSGGS 360

Query: 355  DLRVPHLNKF-QTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQ 413
            D   PH +   Q   LH   NAQQ+ R+   Y+   +    V+++  L+T F   + R Q
Sbjct: 361  DSSHPHSSALAQVSALH--INAQQVSRFLHTYSAATQ-DLQVKELHRLQTLFSKASARYQ 417

Query: 414  TVYN-----EVAFKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLV 468
                     E A  +   +    LR     C E W RF   +M+ G            L 
Sbjct: 418  HFLRDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLA 477

Query: 469  VNNSRPAD--FPHIFKAKEVFYVYLINLAAGVFGYRYFKTFSFKTEEQGVIFFTAISSAV 526
               +      F  +      + +    L AGV       T   K +   +    A  S +
Sbjct: 478  SQLAVAPGFLFRPLLLIPVAWGLTWTILYAGV-----SVTTGSKIDLVVLGAVAAAGSLL 532

Query: 527  ILAFHTLRHWTSIATNWSAVKRFGHMPTRLLLFGSMAVFFSNSFVIQEAKILSYLLAAAI 586
               +       S       +      P  +LL   +A FFS+SF + EA+   +LL + +
Sbjct: 533  PFLWKAWVSRGSKRPLAPLLPV--PRPVLILLLIRLATFFSDSFFVVEARATPFLLGSLV 590

Query: 587  LLLSHEL----------LRLSARLDFRTKFKASQFLRSTALRLILASVLAICLIRFAYTL 636
              L  +L              +RL         +   + AL L +  +L     R A   
Sbjct: 591  FFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHALWLGIGLLL---FTRLAGLF 647

Query: 637  FRCREEQGNCSDFVNTGGAGFSLKKPGTGKTYILAVVVLVVYTTLTRLYLRSCGNLTGNL 696
             RC EE   C            +        Y   V  LV    + RL+LR  GNL    
Sbjct: 648  HRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRYGNLKSPE 707

Query: 697  PNVLLARYGPTVASICAGGHILLANSSIKHIQRTHI------DAMALVIYGLLLVQIIVL 750
            P VL  R+G  +  +    +  LA+ + +   R           +   + GL  + +++L
Sbjct: 708  PPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLL 767

Query: 751  SWAPLMTFV------------LPPRSSHTVTINGNESIVPEIFRKMKRMYEGDDDERRSH 798
             W P+   V            L P S    +    + +VP+I+R M+  ++G  +  ++ 
Sbjct: 768  LWRPVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQ 827

Query: 799  IPVV---YGLATVYSSIVIAFGVFLALVMIVLLEPRASIGLVVCVAVGAILLSVHSILRY 855
             P+    Y L +VYS+ ++   + LA  +++L   R S+  ++      +LL + +    
Sbjct: 828  GPITVAAYQLGSVYSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAA--- 884

Query: 856  RTATSFESCVQPTFTALVGWFLLAHFCFFATSHQTTLSQIEWRAAFVGRTTGIGQSNLVS 915
             T  +        + A+  W LLA   F++T HQ   S I W AAFVG   G G S  + 
Sbjct: 885  GTPVATPGPFTVLWQAVSAWVLLATQTFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLP 944

Query: 916  GALVILNTFCGPIFFFCMYSLLSTETFSLFALFPNLIRSCRSGGKVDASTSMSDLANEAV 975
              LV  NTF   + F     LL    F      P   R    G + +A     +      
Sbjct: 945  ALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKR-RQPLPGSESEARVRPEEEEELQE 1003

Query: 976  GFDMTRGELSLYEYEDVFLGTGFKLATQFFMLQGLKIFCAMLACTIHCRHLMVWKIFAPR 1035
                 R    L +  + F     +L  ++  + G +I    LA +I  RHLMVWK+FAP+
Sbjct: 1004 PLMEVR----LRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHLMVWKVFAPK 1059

Query: 1036 FIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRI 1071
            FI+EA+   VS   L++G  LV+R+   V +  K++
Sbjct: 1060 FIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKL 1095
>dbj|BAB84890.1| (AK074064) FLJ00135 protein [Homo sapiens]
          Length = 597

 Score =  615 bits (1569), Expect = e-174
 Identities = 142/596 (23%), Positives = 226/596 (37%), Gaps = 58/596 (9%)

Query: 377 QIYRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQTVYN-----EVAFKSFVRDLNTN 431
           Q+ R+   Y+   +     +++  L+  F   +   Q +       E    + + +L   
Sbjct: 5   QVSRFLHTYSAATQ-DLQAKELHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQF 63

Query: 432 LRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVVNNSRPADFPHIFKAKEVFYVYL 491
           LR     C E W RF   +M+ G        F   L    +    FP          + L
Sbjct: 64  LRGARAMCIESWARFSLVRMAGGTALLAASCFICLLASQWAISPGFPFCP-------LLL 116

Query: 492 INLAAGVFGYRYFK----TFSFKTEEQGVIFFTAISSAVILAFHTLRHWTSIATNWSAVK 547
             +A G+ G   +     T   K +   +    A+SS +   +     W S     +   
Sbjct: 117 TPVAWGLVGAIAYAGLLGTIELKLDLVLLGAVAAVSSFLPFLWKAWAGWGSKRPLATLFP 176

Query: 548 RFGHMPTRLLLFGSMAVFFSNSFVIQEAKILSYLLAAAILLLSHEL----------LRLS 597
             G  P  LLL   +AVFFS+SFV+ EA+   +LL + ILLL  +L          L   
Sbjct: 177 IPG--PVLLLLLFRLAVFFSDSFVVAEARATPFLLGSFILLLVVQLHWEGQLLPPKLLTM 234

Query: 598 ARLDFRTKFKASQFLRSTALRLILASVLAICLIRFAYTLFRCREEQGNCSDFVNTGGAGF 657
            RL         +   + ALRL +  +L     R A    RC EE   C           
Sbjct: 235 PRLGTSATTNPPRHNGAYALRLGIGLLLC---TRLAGLFHRCPEETPVCHSSPWLSPLAS 291

Query: 658 SLKKPGTGKTYILAVVVLVVYTTLTRLYLRSCGNLTGNLPNVLLARYGPTVASICAGGHI 717
            +        Y   V  LV      RL+LR  GNL    P +L  R+G  + ++    + 
Sbjct: 292 MVGGRAKNLWYGACVAALVALLAAVRLWLRRYGNLKSPEPPMLFVRWGLPLMALGTAAYW 351

Query: 718 LLANSSIKHIQRTHI------DAMALVIYGLLLVQIIVLSWAPLMTF------------V 759
            LA+ + +   R  +        +   + GL    + +L W P+               V
Sbjct: 352 ALASGADEAPPRLRVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLVKAGAGAPRTRTV 411

Query: 760 LPPRSSHTVTINGNESIVPEIFRKMKRMYEGDDDERRSHIPVV---YGLATVYSSIVIAF 816
           L P S    +    + +VP+I+R M+  + G  +  +S  P+    Y L +VYS+ ++  
Sbjct: 412 LTPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQLGSVYSAAMVTA 471

Query: 817 GVFLALVMIVLLEPRASIGLVVCVAVGAILLSVHSI-LRYRTATSFESCVQPTFTALVGW 875
              LA  +++L   R S+  ++      +LL + +  +   T   F    Q    A+  W
Sbjct: 472 LTLLAFPLLLLHAERISLVFLLLFLQSFLLLHLLAAGIPVTTPGPFTVPWQ----AVSAW 527

Query: 876 FLLAHFCFFATSHQTTLSQIEWRAAFVGRTTGIGQSNLVSGALVILNTFCGPIFFF 931
            L+A   F++T HQ     I W AAFVG   G G    +   LV  NTF   + F 
Sbjct: 528 ALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVGANTFASHLLFA 583
>ref|NP_013069.1| (NC_001144) Glycosylphosphatidylinositol (GPI) biosynthesis; Gpi13p
           [Saccharomyces cerevisiae]
 pir||S64782 probable membrane protein YLL031c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97480.1| (Z73136) ORF YLL031c [Saccharomyces cerevisiae]
          Length = 1017

 Score =  549 bits (1402), Expect = e-155
 Identities = 209/719 (29%), Positives = 330/719 (45%), Gaps = 125/719 (17%)

Query: 5   YLFVLIWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEIFKD 64
           Y+ +L+++A L    +  F+RGFLL+R      S+    S  P                 
Sbjct: 35  YIILLVFIAILQFISIAFFTRGFLLSRHVLDNISSQNETSKLP----------------- 77

Query: 65  VNASSNLCLPQKSKVIVLVVDALKYEFGLY--RANATDPLPYENKLVVLQELLQQNPD-H 121
                    P+ +K ++LV+DAL+++F +    +++   L Y N ++ L +    + D  
Sbjct: 78  ---------PRFNKAVILVIDALRFDFAIPVNESHSNYNLNYHNNILSLYDSFASDKDAS 128

Query: 122 ARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVFL 181
           + L++F ADPPTTTLQRLKGLTTGSLPTFID GSNF    I EDN + Q+   +  V F 
Sbjct: 129 SLLLKFIADPPTTTLQRLKGLTTGSLPTFIDAGSNFDGTVIEEDNFLKQLHLANKTVKFA 188

Query: 182 GDSTWTDLYPHRFKRSYSYP--SFDIFDLDSVDNEILKHLPKEL-ESKDWQVLVAHFLGV 238
           GD TW  L+ H F  + S+P  S +++DLD+VDN ++ +    L + K+W V++ H LG+
Sbjct: 189 GDDTWMALF-HPFLSNDSFPLESLNVWDLDTVDNGVMDYFHDHLQQDKEWDVMIGHMLGI 247

Query: 239 DHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDHGMTASGDHGGDTDDET 298
           DH GHK+GP H  M  K  ++++ I  ++ ++D+DT L+++GDHGM  +G+HGGD+ DE 
Sbjct: 248 DHVGHKYGPDHFTMREKQIQVDQFIDWILKSIDDDTLLVILGDHGMDHTGNHGGDSIDEL 307

Query: 299 NALLFAYSKQHRFYG---------NDSGSDSEMLQQIDLVPTLATILGVPIPYSNLGLVN 349
            + LF YSK+   +          ++ G D   ++QIDLV +LA ++G PIP++NLG   
Sbjct: 308 ESTLFLYSKKPDMWRLKETSNYNIDNLGHDYRSVRQIDLVSSLALLMGQPIPFNNLGW-- 365

Query: 350 FNIVPDLRVPHLNKFQTLLLHSWQNAQQIYRYFFQYALEN--------KRTFNVEQMDHL 401
               P   +   ++  +  ++S  +  Q+Y+   Q    N        K   N       
Sbjct: 366 ----PIDEIARNDREWSQFVNSAISQLQLYKDTMQIHHGNDEILEPLAKNISNTPPTSDP 421

Query: 402 ETEFILLTHRVQTVYNEVAFKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFTFLP 461
             +F+ L H+ Q V+                   L TC E+W +FD   ++ G+      
Sbjct: 422 -EKFVKLGHKYQKVF-------------------LQTCEELWAKFDYYSIATGITLLATS 461

Query: 462 LFFIF--------LVVNNSRPADFPHIFKAKEVFYVYLINLAAGVFGYRYFKTFSFKTEE 513
           L  +         +VVN   P   P I        V + NL      Y Y +  SF  + 
Sbjct: 462 LVLLISITKLIPSIVVNQMVPEFVPGII-----IMVLVTNLCFHGIFYVYQQP-SFVDQF 515

Query: 514 QGVIFFTAISSAV---ILAFHTLRH-WTSIATNWSAVKRFGHMPTRLLLFGSMAVFFSNS 569
            G +  TAI   +   I  F      W ++    +    +  +    ++  ++ +F SNS
Sbjct: 516 WGTLLATAIGIIIGCYITIFDRYNFIWIAMRLGETLADYWSRIAVMFMIIHAL-LFTSNS 574

Query: 570 FVIQEAKILSYLLAAAILLLSHELLRLSARLDFRTKFKA------------------SQF 611
           F I E +I+++LL+   +L  +E + L  R        A                  S +
Sbjct: 575 FTIWEDRIVAFLLSTFGMLTLYEFVFLPKRQSTTALLTATISEKEGTTSGVNPSTANSNY 634

Query: 612 LRSTALRLILA---SVLAICLIRFAYTLFRCREEQGN-CS--------DFVNTGGAGFS 658
           L  T    +L    S + I   R A  +  CREEQG  C               G  F 
Sbjct: 635 LPLTRFARLLGGYHSAVLIIFTRLASMITICREEQGEYCIPTFNNQNNSSWWVLGLCFL 693
 Score = 44.3 bits (104), Expect = 0.011
 Identities = 19/64 (29%), Positives = 27/64 (41%)

Query: 990  EDVFLGTGFKLATQFFMLQGLKIFCAMLACTIHCRHLMVWKIFAPRFIYEALATFVSLPA 1049
                LG              +    + +  T   RHLMVWKIF PRFI+ +L+  V+   
Sbjct: 935  PQTLLGRIVSNCGILLTYNTILCLSSFIWVTHFRRHLMVWKIFCPRFIFASLSLIVTQLV 994

Query: 1050 LIVG 1053
            +  G
Sbjct: 995  VTFG 998
 Score = 44.3 bits (104), Expect = 0.012
 Identities = 30/150 (20%), Positives = 56/150 (37%), Gaps = 23/150 (15%)

Query: 796 RSHIPVVYGLATVYSSIVIAFGVFLALVMIVLLEPRASIGLVVCVAVGAILLSVHSILRY 855
           +SH   + G   +Y S      + + + +++  +P A +   +       LLS+  I+  
Sbjct: 795 KSHEATILGYTNIYGSEFFLLVINVLISILLFNKPLAQLSYFLMCNQ---LLSILEIIDL 851

Query: 856 RTATSFESCVQPTFTALVGWFLLAHFCFFATSHQTTLSQIEWRAAF-----VGRTTGIGQ 910
                    ++      +   LL++  FF T HQ T+  ++W   F     V        
Sbjct: 852 LK-------LKENIIGPIALGLLSYQHFFTTGHQATIPSVQWDIGFMLSEKVTFP----- 899

Query: 911 SNLVSGALVILNTFCGPIFFFCMYSLLSTE 940
               +   +ILNTF   I      +LL+  
Sbjct: 900 ---FTQIAIILNTFGPHILVSLSVALLTLW 926
>dbj|BAA82620.1| (AB030280) phosphatidylinositolglycan class N short form [Mus
           musculus]
          Length = 798

 Score =  536 bits (1368), Expect = e-151
 Identities = 93/606 (15%), Positives = 180/606 (29%), Gaps = 89/606 (14%)

Query: 59  NEIFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRA-NATDPLPYENKLVVLQELLQQ 117
           + +   +        P   ++++ V D L+ +       +     P+   +++ +     
Sbjct: 26  SPLVHGMTPQFTPLPPPAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSW-- 83

Query: 118 NPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ------I 171
                         PT +      L  G       +   +    +  D++ ++       
Sbjct: 84  -------GVSHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSW 136

Query: 172 VKNDLPVVFL----GDSTWTDLYPHRFKRSYSYPS--FDIFDLDSVDNEI-----LKHLP 220
              D+  +F     GD  +T  Y  + +   ++ +   D +  D V +        + L 
Sbjct: 137 GSPDILPMFAKGASGDHVYTYSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLF 196

Query: 221 KELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM------DNDT 274
            ++ +++  V   H LGVD  GH H P   E    + ++++ ++ +V+        D  T
Sbjct: 197 TKV-NEEKVVFFLHLLGVDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKT 255

Query: 275 TLLVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSE------------- 321
             +   DHGMT  G HG     ET      +    +F  N S    +             
Sbjct: 256 AFIFTSDHGMTDWGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWK 315

Query: 322 --MLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLRVPHLNKFQTLLLHS-WQNAQQI 378
              + Q D+ P +A+++GVP P +++G++            LN        S + NA QI
Sbjct: 316 RRDVNQADIAPLMASLIGVPFPLNSVGILPVGY--------LNNTGLFKAESMFTNAVQI 367

Query: 379 YRYFFQYALENKR---TFNVEQMDHLETEFILLTHRVQTVYNEV----AFKSFVRDLNTN 431
              F     + K     F       L     L   R    Y +        S   +L   
Sbjct: 368 LEQFKVKMTQKKEATLPFLFTPFKLLSDSQQLDILRKARSYIKQEKFDEVVSLCEELIDL 427

Query: 432 LRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVVNNSRPADFPHIFKAKEVFYVY- 490
               L         F    ++ G +        + +  +++ P       K      +Y 
Sbjct: 428 ALRGLSYYHTYDRLFLGINVAVGFVGWMSYTSLLIIKSHSNIPKGTRKEGKKPHCLLLYS 487

Query: 491 ---------LINLAAGVFGYRYFKTFSFKTEEQGVIFFTAISSAVILAFHTLRHWTSIAT 541
                       +        Y                      +     +L  +     
Sbjct: 488 FIATGVLVACFLMIQACPWTYYVYCL-LPVPIW--YAVLREHEVIQDLVESLLTFPRSHF 544

Query: 542 NWSAVKRFGHMPTRLLLFG-------SMAVFFSNSFVIQ---EAKILSYLLAAAILLLSH 591
               +     +   +L F         + VF    F+ Q    AKI  +L  A   LL  
Sbjct: 545 VAYLLVFTLGIEVLVLSFFYRYMLTAGLIVFAGWPFLTQLWTRAKIT-FLSWAFFSLLLA 603

Query: 592 ELLRLS 597
               + 
Sbjct: 604 VFPLMP 609
>gb|AAH21148.1|AAH21148 (BC021148) Similar to phosphatidylinositol glycan, class N [Mus
           musculus]
          Length = 826

 Score =  533 bits (1359), Expect = e-150
 Identities = 92/606 (15%), Positives = 180/606 (29%), Gaps = 89/606 (14%)

Query: 59  NEIFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRA-NATDPLPYENKLVVLQELLQQ 117
           + +   +        P   ++++ V D L+ +       +     P+   +++ +     
Sbjct: 26  SPLVHGMTPQFTPLPPPAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIIHEGSW-- 83

Query: 118 NPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ------I 171
                         PT +      L  G       +   +    +  D++ ++       
Sbjct: 84  -------GVSHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSW 136

Query: 172 VKNDLPVVFL----GDSTWTDLYPHRFKRSYSYPS--FDIFDLDSVDNEI-----LKHLP 220
              D+  +F     GD  +T  Y  + +   ++ +   D +  D V +        + L 
Sbjct: 137 GSPDILPMFAKGASGDHVYTYSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLF 196

Query: 221 KELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM------DNDT 274
            ++ +++  V   H LG+D  GH H P   E    + ++++ ++ +V+        D  T
Sbjct: 197 TKV-NEEKVVFFLHLLGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKT 255

Query: 275 TLLVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSE------------- 321
             +   DHGMT  G HG     ET      +    +F  N S    +             
Sbjct: 256 AFIFTSDHGMTDWGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWK 315

Query: 322 --MLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLRVPHLNKFQTLLLHS-WQNAQQI 378
              + Q D+ P +A+++GVP P +++G++            LN        S + NA QI
Sbjct: 316 RRDVNQADIAPLMASLIGVPFPLNSVGILPVGY--------LNNTGLFKAESMFTNAVQI 367

Query: 379 YRYFFQYALENKR---TFNVEQMDHLETEFILLTHRVQTVYNEV----AFKSFVRDLNTN 431
              F     + K     F       L     L   R    Y +        S   +L   
Sbjct: 368 LEQFKVKMTQKKEATLPFLFTPFKLLSDSQQLDILRKARSYIKQEKFDEVVSLCEELIDL 427

Query: 432 LRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVVNNSRPADFPHIFKAKEVFYVY- 490
               L         F    ++ G +        + +  +++ P       K      +Y 
Sbjct: 428 ALRGLSYYHTYDRLFLGINVAVGFVGWMSYTSLLIIKSHSNIPKGTRKEGKKPHCLLLYS 487

Query: 491 ---------LINLAAGVFGYRYFKTFSFKTEEQGVIFFTAISSAVILAFHTLRHWTSIAT 541
                       +        Y                      +     +L  +     
Sbjct: 488 FIATGVLVACFLMIQACPWTYYVYCL-LPVPIW--YAVLREHEVIQDLVESLLTFPRSHF 544

Query: 542 NWSAVKRFGHMPTRLLLFG-------SMAVFFSNSFVIQ---EAKILSYLLAAAILLLSH 591
               +     +   +L F         + VF    F+ Q    AKI  +L  A   LL  
Sbjct: 545 VAYLLVFTLGIEVLVLSFFYRYMLTAGLIVFAGWPFLTQLWTRAKIT-FLSWAFFSLLLA 603

Query: 592 ELLRLS 597
               + 
Sbjct: 604 VFPLMP 609
>ref|NP_038812.1| (NM_013784) phosphatidylinositol glycan, class N [Mus musculus]
 dbj|BAA82619.1| (AB030279) phosphatidylinositolglycan class N [Mus musculus]
          Length = 931

 Score =  528 bits (1348), Expect = e-148
 Identities = 93/606 (15%), Positives = 180/606 (29%), Gaps = 89/606 (14%)

Query: 59  NEIFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRA-NATDPLPYENKLVVLQELLQQ 117
           + +   +        P   ++++ V D L+ +       +     P+   +++ +     
Sbjct: 26  SPLVHGMTPQFTPLPPPAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSW-- 83

Query: 118 NPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ------I 171
                         PT +      L  G       +   +    +  D++ ++       
Sbjct: 84  -------GVSHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSW 136

Query: 172 VKNDLPVVFL----GDSTWTDLYPHRFKRSYSYPS--FDIFDLDSVDNEI-----LKHLP 220
              D+  +F     GD  +T  Y  + +   ++ +   D +  D V +        + L 
Sbjct: 137 GSPDILPMFAKGASGDHVYTYSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLF 196

Query: 221 KELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM------DNDT 274
            ++ +++  V   H LGVD  GH H P   E    + ++++ ++ +V+        D  T
Sbjct: 197 TKV-NEEKVVFFLHLLGVDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKT 255

Query: 275 TLLVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSE------------- 321
             +   DHGMT  G HG     ET      +    +F  N S    +             
Sbjct: 256 AFIFTSDHGMTDWGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWK 315

Query: 322 --MLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLRVPHLNKFQTLLLHS-WQNAQQI 378
              + Q D+ P +A+++GVP P +++G++            LN        S + NA QI
Sbjct: 316 RRDVNQADIAPLMASLIGVPFPLNSVGILPVGY--------LNNTGLFKAESMFTNAVQI 367

Query: 379 YRYFFQYALENKR---TFNVEQMDHLETEFILLTHRVQTVYNEV----AFKSFVRDLNTN 431
              F     + K     F       L     L   R    Y +        S   +L   
Sbjct: 368 LEQFKVKMTQKKEATLPFLFTPFKLLSDSQQLDILRKARSYIKQEKFDEVVSLCEELIDL 427

Query: 432 LRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVVNNSRPADFPHIFKAKEVFYVY- 490
               L         F    ++ G +        + +  +++ P       K      +Y 
Sbjct: 428 ALRGLSYYHTYDRLFLGINVAVGFVGWMSYTSLLIIKSHSNIPKGTRKEGKKPHCLLLYS 487

Query: 491 ---------LINLAAGVFGYRYFKTFSFKTEEQGVIFFTAISSAVILAFHTLRHWTSIAT 541
                       +        Y                      +     +L  +     
Sbjct: 488 FIATGVLVACFLMIQACPWTYYVYCL-LPVPIW--YAVLREHEVIQDLVESLLTFPRSHF 544

Query: 542 NWSAVKRFGHMPTRLLLFG-------SMAVFFSNSFVIQ---EAKILSYLLAAAILLLSH 591
               +     +   +L F         + VF    F+ Q    AKI  +L  A   LL  
Sbjct: 545 VAYLLVFTLGIEVLVLSFFYRYMLTAGLIVFAGWPFLTQLWTRAKIT-FLSWAFFSLLLA 603

Query: 592 ELLRLS 597
               + 
Sbjct: 604 VFPLMP 609
>ref|NP_491750.1| (NM_059349) C27A12.9.p [Caenorhabditis elegans]
 gb|AAB93646.1| (AF003137) Hypothetical protein C27A12.9 [Caenorhabditis elegans]
          Length = 905

 Score =  516 bits (1315), Expect = e-145
 Identities = 179/695 (25%), Positives = 303/695 (42%), Gaps = 133/695 (19%)

Query: 6   LFVLIWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEIFKDV 65
           +  ++ ++  I   + +F  GFLL R      S+C      P                  
Sbjct: 4   IIRILLVSINILLALFIFQNGFLLKRQEILSKSSCSDAHAQPGQT--------------- 48

Query: 66  NASSNLCLPQKSKVIVLVVDALKYEFGLYRANA---TDPLPYENKLVVLQELLQQNPDHA 122
                    Q  +VI+++VDAL+Y+F +        +    Y    +   E L ++  + 
Sbjct: 49  ----CWMKQQYQRVILILVDALRYDFLIPIDENTKKSKEEWYYRGQMKNIEKLVKSG-NV 103

Query: 123 RLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEI-NEDNIIDQIVKNDLPVVFL 181
            +    ADPPTTTLQRLK LTTG+LPTFID G NF+   + +ED+ + Q  +    V  L
Sbjct: 104 SIGTLLADPPTTTLQRLKALTTGTLPTFIDAGDNFSPDAVISEDSFVYQAAQLGKNVTLL 163

Query: 182 GDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLP------------------KEL 223
           GD TW  L+P++F ++ +Y SFDI DL++VD++I + +                    E+
Sbjct: 164 GDDTWLSLFPNQFSKTAAYDSFDINDLNTVDDKIARKIFFGKYYNKMVFCFEGPILQDEM 223

Query: 224 ESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDHG 283
            + +  +++AHFLGVDHCGHK GP H  MA  L +M+ +I   +  M +D       DHG
Sbjct: 224 LNSNSSIIIAHFLGVDHCGHKFGPSHPVMADTLRKMDRIIGQTIETMKSD-------DHG 276

Query: 284 MTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPYS 343
           MT++GDHGG++++E  A +  +SK+H+        +   + QID+VPT++ ++G+PIP+S
Sbjct: 277 MTSTGDHGGESENEIRAGILVHSKKHQI----ILPERP-IHQIDIVPTISLLMGLPIPFS 331

Query: 344 NLGLVNFNIVPDLRVPHLNKFQTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMDHLET 403
           NLG V                Q      W+ A+Q+  + +Q ++                
Sbjct: 332 NLGTVI--------------TQLFTRDLWEIARQMMGFVWQDSVG--------------- 362

Query: 404 EFILLTHRVQTVYNEVAFKSF-----VRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFT 458
                T+  Q  + E+   +      + +    +  I    R  W +FD   ++ G+   
Sbjct: 363 -IFAETYAAQKNFGELHSHTIRDSNTMEEQLDTMSRIQTLLRVSWTQFDDAYINAGIFSL 421

Query: 459 FLPLFFIFLVVNNSRPADFPHIFKAKEVFYVYLINLAAGVFGYRYFKTFSFKTE------ 512
              + F+                   EV  +  I    G  G       + KT+      
Sbjct: 422 VEAIMFLI----------------TNEVMSLEWIIYRTGC-GLLQAALLTDKTDSDGSAR 464

Query: 513 --EQGVIFFTAISSAVILAFHTLRHWTSIATNWSAVKRFGHMPTRLLLFGSMAVFFSNSF 570
                 +  + +SS + +    L+   SI +  S  K  G +   L++  S+++F SNS+
Sbjct: 465 TLLLMTLAVSCLSSIISMCHKALQIRISIKSMLS-AKVIGPI---LVIIHSISLF-SNSY 519

Query: 571 VIQEAKILSYLLAAAILLLSHELLRLSARLDFRTKFKASQFLRSTALRL-------ILAS 623
           V+ EA+++ Y L + + L+  E LR        T  +  +  +   L         ++  
Sbjct: 520 VVYEAQVVRYFLQSLVTLVFLEKLR-------STALRPRKPFKLNFLWKFVNQDQNLIIF 572

Query: 624 VLAICLIRFAYTLFRCREEQGNCSDFVNTGGAGFS 658
            L +  +R      RCREE+  C  +  +  AG  
Sbjct: 573 TLIMVGLRSEPIFHRCREEEVGCDTYYPSRQAGSL 607
 Score = 79.4 bits (194), Expect = 3e-13
 Identities = 21/44 (47%), Positives = 29/44 (65%)

Query: 1002 TQFFMLQGLKIFCAMLACTIHCRHLMVWKIFAPRFIYEALATFV 1045
            T+  +L  ++     L  +IH RHLMVWKIFAP+FI+E + T V
Sbjct: 832  TETLVLMTIRATLTCLVSSIHRRHLMVWKIFAPKFIFECILTIV 875
 Score = 36.2 bits (83), Expect = 3.0
 Identities = 31/162 (19%), Positives = 51/162 (31%), Gaps = 25/162 (15%)

Query: 782 RKMKRMYEGDDDERRSHIPVVYGLATVYSSIVIAFGVFLALVMIV----LLEPRASIGLV 837
           R  K   E +       I ++   + ++ +I++   VF +L   V    L     S+  +
Sbjct: 637 RDTKSQIESNSKRSSETIRILSATSWLHIAIIVLHSVFASLTHDVSRANLSAHSLSVISL 696

Query: 838 VCVAV------GAILLSVHSILRYRTATSFESCVQPTFTALVGW--------------FL 877
           +C  +       AI      +  Y             F A+                  L
Sbjct: 697 ICAVLAWREGNSAICAHFLLMPVYILFGDGLIPAIIIFIAVSVLIAEFVSREFIASTIAL 756

Query: 878 LAHFCFFATSHQTTLSQIEWRAAFVGRTTGIGQSNLVSGALV 919
           L    F+   H   +S I W AAFVG   G     ++    V
Sbjct: 757 LIPLGFYHLGHSPVISSIPWNAAFVGIPGGT-ALRVLPALFV 797
>pir||T40030 hypothetical protein SPBC27B12.06 - fission yeast
           (Schizosaccharomyces pombe)
 dbj|BAA21454.1| (AB004539) ORF YLL031c [Schizosaccharomyces pombe]
 emb|CAA16901.1| (AL021766) hypothetical protein [Schizosaccharomyces pombe]
          Length = 918

 Score =  497 bits (1268), Expect = e-139
 Identities = 194/708 (27%), Positives = 299/708 (41%), Gaps = 112/708 (15%)

Query: 5   YLFVLIWLAFLISS---GVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEI 61
           Y+   I+ A ++ S   G+  F+ GFLL R    +TS C                     
Sbjct: 21  YIQACIFFAIILISNFYGLKSFTDGFLLRRAVLNQTSLCENPPA---------------D 65

Query: 62  FKDVNASS-NLCLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPD 120
            ++   SS         + +++++DAL+Y+F +   ++     Y N      E    +P+
Sbjct: 66  VREWKNSSGCWAPKIFERAVIVIIDALRYDFLIPYNDSNY---YHNAFTTPYETSVLHPE 122

Query: 121 HARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVF 180
           ++ L +F AD PTTT QRLKGLTTGSLPTFID+GSNFA   I+EDN++ Q    D  +V 
Sbjct: 123 NSYLTQFIADAPTTTSQRLKGLTTGSLPTFIDLGSNFAGTNIDEDNLLLQWKSLDKQIVL 182

Query: 181 LGDSTWTDLYPHRFKRSYSYP--SFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGV 238
           LGD TW  L+      + S P  SF++ DL  VDN++ +++   ++  ++ VL+AH+LGV
Sbjct: 183 LGDDTWDVLFHDYLNETLSQPAFSFNVPDLHGVDNKVNQYVFDYIKDANFDVLIAHYLGV 242

Query: 239 DHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDHGMTASGDHGGDTDDET 298
           DH GH+ GP H  M  KL +M+  ++ ++  +D+ T L+VMGDHGM   G+HGGD+ DE 
Sbjct: 243 DHVGHRLGPDHPTMRDKLNQMDRCVKEMMDLLDDSTLLIVMGDHGMDNKGNHGGDSFDEI 302

Query: 299 NALLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLRV 358
           N++L+ YSK+  F             Q+DLVPTL+ +LG PIPY NLG +          
Sbjct: 303 NSVLWMYSKKPTFGYLKQPGKVLSANQVDLVPTLSLLLGNPIPYGNLGTLIPEPFY---- 358

Query: 359 PHLNKFQTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQTVYNE 418
            +               Q + R+F +Y L+          D L +      H+    Y +
Sbjct: 359 -YYGDEYLSKAQKINIGQ-LNRFFSEYDLDAS--------DFLSSSV----HKNNNSYLD 404

Query: 419 VAFKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFTFLPLF----------FIFLV 468
             F  F        RD     + IW  F    M  G L   +  F           IF +
Sbjct: 405 QYFLDF-----DYARDAFSYFKAIWAEFSLFPMIIGFLLLIIGGFNLALLMQDKSVIFRM 459

Query: 469 VNNSRPADFPHIFKAKEVFYVYLINLAAGVFGY--RYFKTFSFKTEEQGVIFFTAISSAV 526
             N  P+             + L N           Y    SF       I   + +  +
Sbjct: 460 SANMAPS-VMKC--LPVCLILILANNELHSPFPAEFYVLLPSF------YILLNSFNQKL 510

Query: 527 ILAFHTLRHWTSIATNWSAVKRFGHMPTRLLLFGSMAVFFSNSFVIQEAKILSYLLAAAI 586
           +  F               +  F       + F  +  F SNSF + E ++  +L+    
Sbjct: 511 MEYFKGFVK----------LDYFS----IFITFLHVCSFGSNSFTVWEDRLCHFLIITIG 556

Query: 587 LLLSHELLRLSARLDFRTKFKASQFLRSTALRLILASVLAICLIRFAYTLFRCREEQGN- 645
           L++  +             F  S +    AL  IL  V+       +  +  CREEQG  
Sbjct: 557 LVMFCKCFSEM-----SPLFACSTYS---ALAFILLQVI-------SSYVTNCREEQGAF 601

Query: 646 CSDFVNTGGAGFSLKKPGTGKTYILAVVVLVVYTTLT-----RLYLRS 688
           C     +                 L V+ L+  +++      +L+LR 
Sbjct: 602 CVSTYISTP---------DNSLRTLIVLALMALSSIILPLILQLHLRR 640
>ref|NP_036459.1| (NM_012327) phosphatidylinositol glycan, class N; endoplasmic
           reticulum membrane protein [Homo sapiens]
 gb|AAD11432.1| (AF109219) Mcd4p homolog [Homo sapiens]
          Length = 931

 Score =  488 bits (1243), Expect = e-136
 Identities = 90/611 (14%), Positives = 185/611 (29%), Gaps = 99/611 (16%)

Query: 59  NEIFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRANA-TDPLPYENKLVVLQELLQQ 117
           + +   +        P   ++++ V D L+ +             P+   +++ +     
Sbjct: 26  SPLVHGMTPQFTPLPPPARRLVLFVADGLRADALYELDENGNSRAPFIRNIIMHEGSW-- 83

Query: 118 NPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ------I 171
                         PT +      L  G       +   +    +  D++ ++       
Sbjct: 84  -------GISHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSW 136

Query: 172 VKNDLPVVFL----GDSTWTDLYPHRFKRSYSYPS--FDIFDLDSVDNEI-----LKHLP 220
              D+  +F     GD  +T  Y  + +   +  +   D +  D+V +        + L 
Sbjct: 137 GSPDILPMFAKGASGDHVYTYSYDAKREDFGAQDATKLDTWVFDNVKDFFHHARNNQSLF 196

Query: 221 KELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM------DNDT 274
            ++ +++  V   H LG+D  GH H P   +    + ++++ ++ +V+        D  T
Sbjct: 197 SKI-NEEKIVFFLHLLGIDTNGHAHRPSSRDYKHNIKKVDDGVKEIVSMFNHFYGNDGKT 255

Query: 275 TLLVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSE------------- 321
           T +   DHGMT  G HG     ET   L  +    ++    S    +             
Sbjct: 256 TFIFTSDHGMTDWGSHGAGHPSETLTPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWK 315

Query: 322 --MLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLRVPHLNKFQTLLLHS-WQNAQQI 378
              + Q D+ P + +++GVP P +++G++  +         LN        S + NA QI
Sbjct: 316 RLDVNQADIAPLMTSLIGVPFPLNSVGILPVDY--------LNNTDLFKAESMFTNAVQI 367

Query: 379 YRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQTVYNEV-----------AFKSFVRD 427
              F     + K       +  L T F LL+   Q                    S  ++
Sbjct: 368 LEQFKVKMTQKKEVT----LPFLFTPFKLLSDSKQFNILRKARSYIKHRKFDEVVSLCKE 423

Query: 428 LNTNLRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVVNNS----------RPADF 477
           L       L         F    +  G +        + +  +++          +P+  
Sbjct: 424 LIHLALKGLSYYHTYDRFFLGVNVVIGFVGWISYASLLIIKSHSNLIKGVSKEVKKPSHL 483

Query: 478 PHIFKAKEVFYVYLINLAAGVFGYRYFK-TFSFKTEEQGVIFFTAISSAVILAFHTLRHW 536
                      V    L        Y             +  F  I   V+    ++  +
Sbjct: 484 LPCSFVAIGILVAFFLLIQACPWTYYVYGLLPLPIWYAVLREFQVIQDLVV----SVLTY 539

Query: 537 TSIATNWSAVKRFGHMPTRLLLFG-------SMAVFFSNSFVIQ---EAKILSYLLAAAI 586
                    +     +   +L F         +  F +  F+ +    AK+ S L     
Sbjct: 540 PLSHFVGYLLAFTLGIEVLVLSFFYRYMLTAGLTAFAAWPFLTRLWTRAKMTS-LSWTFF 598

Query: 587 LLLSHELLRLS 597
            LL      + 
Sbjct: 599 SLLLAVFPLMP 609
>pir||T02245 hypothetical protein P1.11659_3 - human
 gb|AAC07985.1| (AC004472) P1.11659_3 [Homo sapiens]
          Length = 880

 Score =  483 bits (1232), Expect = e-135
 Identities = 182/504 (36%), Positives = 273/504 (54%), Gaps = 46/504 (9%)

Query: 4   TYLFVLIWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEIFK 63
           + L  L W+ FL  +G+ LF+ GFLL R+  T  S+C+     P +    +         
Sbjct: 5   SVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPG-PGSLPWGSQG------- 56

Query: 64  DVNASSNLCLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHAR 123
                +     + S+V+++++DAL+++F   + +                +L+  P HAR
Sbjct: 57  --KPGACWMASRFSRVVLVLIDALRFDFAQPQHSH--------------RILEIQPHHAR 100

Query: 124 LMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVFLGD 183
           L R + DPPTTT+QRLK LTTGSLPTFID GSNFAS  I EDN+I Q+      VVF+GD
Sbjct: 101 LYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGD 160

Query: 184 STWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDHCGH 243
            TW DL+P  F +++ +PSF++ DLD+VDN IL+HL   ++S +W VL+AHFLGVDHCGH
Sbjct: 161 DTWKDLFPGAFSKAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGH 220

Query: 244 KHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDHGMTASGDHGGDTDDETNALLF 303
           KHGP H EMA+KL +M++VI+ +V  ++NDT L+V GDHGMT +GDHGGD++ E +A LF
Sbjct: 221 KHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALF 280

Query: 304 AYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPYSNLGLVNFNIVP--DLRVPHL 361
            YS    F  +    + E++ Q+ LVPTLA +LG+PIP+ N+G V   +    +   PH 
Sbjct: 281 LYSPTAVFP-STPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHS 339

Query: 362 NKF-QTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQTVYN--- 417
           +   Q   LH   NAQQ+ R+   Y+   +     +++  L+  F   +   Q +     
Sbjct: 340 SALAQASALH--LNAQQVSRFLHTYSAATQ-DLQAKELHQLQNLFSKASADYQWLLQSPK 396

Query: 418 --EVAFKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFTFLPL----------FFI 465
             E    + + +L   LR     C E W RF   +M+      +               +
Sbjct: 397 GAEATLPTVIAELQQFLRGARAMCIESWARFSLVRMAGAKNLWYGACVAALVALLAAVRL 456

Query: 466 FLVVNNSRPADFPHIFKAKEVFYV 489
           +L    +  +  P +   +    +
Sbjct: 457 WLRRYGNLKSPEPPMLFVRWGLPL 480
 Score =  246 bits (622), Expect = 2e-63
 Identities = 49/238 (20%), Positives = 91/238 (37%), Gaps = 24/238 (10%)

Query: 646 CSDFVNTGGAGFSLKKPGTGKTYILAVVVLVVYTTLTRLYLRSCGNLTGNLPNVLLARYG 705
           C +                   Y   V  LV      RL+LR  GNL    P +L  R+G
Sbjct: 420 CIESWARFSLVRMAG--AKNLWYGACVAALVALLAAVRLWLRRYGNLKSPEPPMLFVRWG 477

Query: 706 PTVASICAGGHILLANSSIKHIQRTHI------DAMALVIYGLLLVQIIVLSWAPLMTF- 758
             + ++    +  LA+ + +   R  +        +   + GL    + +L W P+    
Sbjct: 478 LPLMALGTAAYWALASGADEAPPRLRVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLV 537

Query: 759 -----------VLPPRSSHTVTINGNESIVPEIFRKMKRMYEGDDDERRSHIPVV---YG 804
                      VL P S    +    + +VP+I+R M+  + G  +  +S  P+    Y 
Sbjct: 538 KAGAGAPRTRTVLTPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQ 597

Query: 805 LATVYSSIVIAFGVFLALVMIVLLEPRASIGLVVCVAVGAILLSVHSI-LRYRTATSF 861
           L +VYS+ ++     LA  +++L   R S+  ++      +LL + +  +   T   +
Sbjct: 598 LGSVYSAAMVTALTLLAFPLLLLHAERISLVFLLLFLQSFLLLHLLAAGIPVTTPGKY 655
 Score =  224 bits (566), Expect = 8e-57
 Identities = 37/159 (23%), Positives = 55/159 (34%), Gaps = 14/159 (8%)

Query: 854  RYRTATSFESCVQPTFTALVGWFLLAHFCFFATSHQTTLSQIEWRAAFVGRTTGIGQSNL 913
            R + ++         + A+  W L+A   F++T HQ     I W AAFVG   G G    
Sbjct: 724  RDKESSKTPCPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTW 783

Query: 914  VSGALVILNTFCGPIFFFCMYSLLSTETFSLFALFPNLIRSCRSGGKVDASTSMSDLANE 973
            +   LV  NTF   + F     LL    F            C S G         + A+ 
Sbjct: 784  LPALLVGANTFASHLLFAVGCPLLLLWPFL-----------CESQGLRKRQQPPGNEADA 832

Query: 974  AVGFDMTRGELS---LYEYEDVFLGTGFKLATQFFMLQG 1009
             V  +     L    L +    F     +L  ++  + G
Sbjct: 833  RVRPEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILG 871
>ref|NP_012756.1| (NC_001143) Required for GPI anchor synthesis; Mcd4p [Saccharomyces
           cerevisiae]
 sp|P36051|YKQ5_YEAST HYPOTHETICAL 105.7 KD PROTEIN IN TPK3-PIR1 INTERGENIC REGION
 pir||S37786 hypothetical protein YKL165c - yeast (Saccharomyces cerevisiae)
 emb|CAA81489.1| (Z26877) unknown [Saccharomyces cerevisiae]
 emb|CAA82007.1| (Z28165) ORF YKL165c [Saccharomyces cerevisiae]
 prf||2118404A ORF [Saccharomyces cerevisiae]
          Length = 919

 Score =  447 bits (1139), Expect = e-124
 Identities = 85/552 (15%), Positives = 177/552 (31%), Gaps = 72/552 (13%)

Query: 59  NEIFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRANATDP------LPYENKLVVLQ 112
           + +   ++   +   P   ++ ++V D L+ +    +             P+   LV+  
Sbjct: 31  SPLVHGMSPYQSTPTPPAKRLFLIVGDGLRADTTFDKVTHPVSGKTEFLAPFIRSLVMN- 89

Query: 113 ELLQQNPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ-- 170
                   +A         PT +      +  G       +   + S  +N D+  +Q  
Sbjct: 90  --------NATYGISHTRMPTESRPGHVAMIAGFYEDVSAVTKGWKSNPVNFDSFFNQST 141

Query: 171 ----IVKNDLPVVF---LGD----STWTDLYPHRFKRSYSY----PSFDIFDLDSVDNEI 215
                   D+  +F     D     TW   Y H F+          +F    LD + +  
Sbjct: 142 HTYSFGSPDILPMFKDGASDPNKVDTWM--YDHTFEDFTQSSIELDAFVFRHLDQLFHNS 199

Query: 216 LKH-LPKELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM---- 270
             +        +D  V   H LG D  GH + P   E    +  +++ I  ++  +    
Sbjct: 200 TLNSTLDYEIRQDGNVFFLHLLGCDTAGHSYRPYSAEYYDNVKYIDDQIPILIDKVNKFF 259

Query: 271 -DNDTTLLVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSE-------- 321
            D+ T  +   DHGM+A G HG    + T   L A+        ++    S+        
Sbjct: 260 ADDKTAFIFTADHGMSAFGSHGDGHPNNTRTPLVAWGAGLNKPVHNPFPVSDNYTENWEL 319

Query: 322 ------MLQQIDLVPTLATILGVPIPYSNLGLVNFNIVP----DLRVPHLNKFQTLLLHS 371
                  ++Q D+   ++ ++GV  P +++G +    +     D      N  +++L   
Sbjct: 320 SSIKRNDVKQADIASLMSYLIGVNYPKNSVGELPIAYIDGKESDKLAALYNNARSILEQY 379

Query: 372 WQNAQQI--YRYFFQYALENKRTFNVEQMDHLETEFILLTHRVQTVYNEVAFKSFVRDLN 429
                ++   ++F++   +     +   ++ +ET    ++      Y E    +   +L 
Sbjct: 380 LVKQDEVIDSQFFYKEYFKFVEKSHSHYLEEIETLIQRISEG--ENYLEQEAITLTEELM 437

Query: 430 TNLRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVV-------NNSRPADFPHIFK 482
               + L        RF  T ++ G +      F IFL         ++ + +   H   
Sbjct: 438 QITLEGLHYLTTYNWRFIRTIVTFGFVGWIFFSFIIFLKSFILENVIDDQKASPLSHAVF 497

Query: 483 AKEVFYVYLINLAAGVFGYRYFKTFSFKTEEQGVIFF--TAISSAVILAFHTLRHWTSIA 540
                 +  I          Y     F       IF   + + S +   F     W  + 
Sbjct: 498 GSIGILLNWILFYQHSPFNFYMYLL-FPLYFWSYIFTNRSVLRSGIKEFFKGTSPWKRVL 556

Query: 541 TNWSAVKRFGHM 552
              S +  +  +
Sbjct: 557 ITISIISVYEGI 568
>ref|NP_186787.1| (NM_111004) putative phosphatidylinositolglycan class N short form
           [Arabidopsis thaliana]
 gb|AAF24617.1|AC010870_10 (AC010870) putative phosphatidylinositolglycan class N short form
           [Arabidopsis thaliana]
          Length = 921

 Score =  410 bits (1043), Expect = e-113
 Identities = 90/594 (15%), Positives = 182/594 (30%), Gaps = 101/594 (17%)

Query: 59  NEIFKDVNA-SSNLCLPQKSKVIVLVVDALKYEFGLYRA-NATDPLPYENKLVVLQELLQ 116
             I   ++        P   ++++L+ D L+ +             P+   ++  Q    
Sbjct: 68  TPIVHGMDPVPPRFSEPPAKRLVLLISDGLRADKFFEPDEEGKYRAPFLRNIIKNQGRW- 126

Query: 117 QNPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ------ 170
                       A PPT +      +  G       +   + +  +  D++ +Q      
Sbjct: 127 --------GVSHARPPTESRPGHVAIIAGFYEDPSAVTKGWKANPVEFDSVFNQSRHTFA 178

Query: 171 IVKNDLPVVF---LGDSTWTDLYPHRFKRSYSYPSF-DIFDLDSVDNEILKHLPK----E 222
               D+  +F   L  STW   YPH ++   +  SF D +  D  +  + +        E
Sbjct: 179 FGSPDIIPIFCSALPHSTWN-SYPHEYEDFATDASFLDEWSFDQFEGLLNRSHADPKLKE 237

Query: 223 LESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM-----DNDTTLL 277
           L  +D  V+  H LG D  GH H P        +  ++++   V   +     DN T+ +
Sbjct: 238 LLHQDKLVVFLHLLGCDSNGHAHRPYSSIYLNNVKVVDKIAERVYHLLEDYYRDNRTSYI 297

Query: 278 VMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSG-------------------- 317
              DHGM+  G HG      T+  L A+    ++    SG                    
Sbjct: 298 FTADHGMSDKGSHGDGHPTNTDTPLVAWGAGIQYPKPASGNSHSDSVTTFVDKHAHDMPT 357

Query: 318 --------SDSEMLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLRVPHLNKFQTLLL 369
                    +   + Q D+ P ++T+LG+P P +++G +    +       LN+ + +  
Sbjct: 358 PYDWGLRRVERVDVNQADIAPLMSTLLGLPCPVNSVGNLPLGYMK------LNEAEEVEA 411

Query: 370 HSWQNAQQIYRYFFQYALENKRTF----------NVEQMDHLETEFILLTHRVQTVYNEV 419
                 Q + +   +  +++  +           +   +  ++      ++         
Sbjct: 412 VVANTKQILNQLLRKSYIKSSNSLFFKPFKPLVHHSFSLSQIDELISAKSY--------E 463

Query: 420 AFKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVVNNSRPADFPH 479
           A      DL     + L   +        T ++ G     + L    L   +S   D   
Sbjct: 464 AAMKLAVDLRNLSLEGLHYFQTYDWLMLMTVITLGYTGWMIVLALHVLQCYSSLSGDLSK 523

Query: 480 IFKAKEVFY-------VYLINLAAGVFGYRYFKTFSFKTEEQGVIFFTAISSAVILAFHT 532
                           + ++NL        Y             IF  +    +   +  
Sbjct: 524 KEHLSVYISGCLLMAILSVLNLVEHSP-PLYHAYIGMTVFLWTQIF--SEYRLIRGLWKY 580

Query: 533 LRHWTSIATNWSAVKRFGHMPTRLLLFGSMAVFFSNSFVIQEAKILSYLLAAAI 586
           LR   +             +    LL          SF  ++     +L+A  +
Sbjct: 581 LRERRADYFIKLLFAAAVAVVIVELLVH--------SFTERKLYTWFFLIAGVV 626
 Score =  267 bits (677), Expect = 7e-70
 Identities = 55/280 (19%), Positives = 100/280 (35%), Gaps = 31/280 (11%)

Query: 59  NEIFKDVNA-SSNLCLPQKSKVIVLVVDALKYEFGLYRA-NATDPLPYENKLVVLQELLQ 116
             I   ++        P   ++++L+ D L+ +             P+   ++  Q    
Sbjct: 68  TPIVHGMDPVPPRFSEPPAKRLVLLISDGLRADKFFEPDEEGKYRAPFLRNIIKNQGRW- 126

Query: 117 QNPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ------ 170
                       A PPT +      +  G       +   + +  +  D++ +Q      
Sbjct: 127 --------GVSHARPPTESRPGHVAIIAGFYEDPSAVTKGWKANPVEFDSVFNQSRHTFA 178

Query: 171 IVKNDLPVVF---LGDSTWTDLYPHRFKRSYSYPSF-DIFDLDSVDNEILKHLPK----E 222
               D+  +F   L  STW   YPH ++   +  SF D +  D  +  + +        E
Sbjct: 179 FGSPDIIPIFCSALPHSTWN-SYPHEYEDFATDASFLDEWSFDQFEGLLNRSHADPKLKE 237

Query: 223 LESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM-----DNDTTLL 277
           L  +D  V+  H LG D  GH H P        +  ++++   V   +     DN T+ +
Sbjct: 238 LLHQDKLVVFLHLLGCDSNGHAHRPYSSIYLNNVKVVDKIAERVYHLLEDYYRDNRTSYI 297

Query: 278 VMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSG 317
              DHGM+  G HG      T+  L A+    ++    SG
Sbjct: 298 FTADHGMSDKGSHGDGHPTNTDTPLVAWGAGIQYPKPASG 337
>pir||T46311 hypothetical protein DKFZp434H0711.1 - human (fragment)
 emb|CAB70839.1| (AL137607) hypothetical protein [Homo sapiens]
          Length = 825

 Score =  388 bits (987), Expect = e-106
 Identities = 81/521 (15%), Positives = 161/521 (30%), Gaps = 89/521 (17%)

Query: 148 PTFIDIGSNFASPEINEDNIIDQ------IVKNDLPVVFL----GDSTWTDLYPHRFKRS 197
                +   +    +  D++ ++          D+  +F     GD  +T  Y  + +  
Sbjct: 1   EDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGASGDHVYTYSYDAKREDF 60

Query: 198 YSYPS--FDIFDLDSVDNEI-----LKHLPKELESKDWQVLVAHFLGVDHCGHKHGPMHE 250
            +  +   D +  D+V +        + L  ++ +++  V   H LG+D  GH H P   
Sbjct: 61  GAQDATKLDTWVFDNVKDFFHHARNNQSLFSKI-NEEKIVFFLHLLGIDTNGHAHRPSSR 119

Query: 251 EMARKLGEMNEVIRSVVAAM------DNDTTLLVMGDHGMTASGDHGGDTDDETNALLFA 304
           +    + ++++ ++ +V+        D  TT +   DHGMT  G HG     ET   L  
Sbjct: 120 DYKHNIKKVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHPSETLTPLVT 179

Query: 305 YSKQHRFYGNDSGSDSE---------------MLQQIDLVPTLATILGVPIPYSNLGLVN 349
           +    ++    S    +                + Q D+ P + +++GVP P +++G++ 
Sbjct: 180 WGAGIKYPQRVSAQQFDDAFLKEWRLENWKRLDVNQADIAPLMTSLIGVPFPLNSVGILP 239

Query: 350 FNIVPDLRVPHLNKFQTLLLHS-WQNAQQIYRYFFQYALENKRTFNVEQMDHLETEFILL 408
            +         LN        S + NA QI   F     + K       +  L T F LL
Sbjct: 240 VDY--------LNNTDLFKAESMFTNAVQILEQFKVKMTQKKEVT----LPFLFTPFKLL 287

Query: 409 THRVQTVYNEV-----------AFKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLF 457
           +   Q                    S  ++L       L         F    +  G + 
Sbjct: 288 SDSKQFNILRKARSYIKHRKFDEVVSLCKELIHLALKGLSYYHTYDRFFLGVNVVIGFVG 347

Query: 458 TFLPLFFIFLVVNNS----------RPADFPHIFKAKEVFYVYLINLAAGVFGYRYFK-T 506
                  + +  +++          +P+             V    L        Y    
Sbjct: 348 WISYASLLIIKSHSNLIKGVSKEVKKPSHLLPCSFVAIGILVAFFLLIQACPWTYYVYGL 407

Query: 507 FSFKTEEQGVIFFTAISSAVILAFHTLRHWTSIATNWSAVKRFGHMPTRLLLFG------ 560
                    +  F  I   V+    ++  +         +     +   +L F       
Sbjct: 408 LPLPIWYAVLREFQVIQDLVV----SVLTYPLSHFVGYLLAFTLGIEVLVLSFFYRYMLT 463

Query: 561 -SMAVFFSNSFVIQ---EAKILSYLLAAAILLLSHELLRLS 597
             +  F +  F+ +    AK+ S L      LL      + 
Sbjct: 464 AGLTAFAAWPFLTRLWTRAKMTS-LSWTFFSLLLAVFPLMP 503
>gb|AAF58853.1| (AE003831) CG2292 gene product [Drosophila melanogaster]
          Length = 874

 Score =  378 bits (963), Expect = e-103
 Identities = 92/591 (15%), Positives = 175/591 (29%), Gaps = 81/591 (13%)

Query: 59  NEIFKDVNASSNL----CLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQEL 114
           + +   +    +L      P  ++++++V D  + +           +P   K+ + + +
Sbjct: 24  SPVITGLTPQKHLLYYGLEPPANRLVLIVTDGFRADSFFE--ENCRYVPNLRKIFLREGV 81

Query: 115 LQQNPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ---- 170
                    +   R   PT T      L  G       +   + S  I+ D + ++    
Sbjct: 82  ---------VGVSRTRVPTETRPGHITLIAGLYEDPSAVLRGWKSNPIDFDTVFNRSSQT 132

Query: 171 --IVKNDLPVVFL----GDSTWTDLYPHRFKRSYSYPSF--DIFDLDSVDNEILKHLPKE 222
                ND+  VF     G       Y H    S  Y ++  D +    V   + +     
Sbjct: 133 YAWGANDVLNVFSHVSNGGEINLRFYNHDLDFSPGYDAYEEDEWVFKRVKLLLQQKRE-A 191

Query: 223 LESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM-----DNDTTLL 277
           L+     V   H LG+D  GH H P   +  R L +  + + ++         D  T  L
Sbjct: 192 LQRAQNVVFFLHLLGLDTAGHVHKPGAPKFRRTLEKTEKGVYAIYQEFERVFPDKRTAYL 251

Query: 278 VMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSEM--LQQIDLVPTLATI 335
           +  DHGMT SG           +          F  N+ G    +  L+Q  L P ++ +
Sbjct: 252 LTADHGMTDSGA----------SRAVPKPGGRTFMPNNEGPAMPLHELEQAQLTPLMSAL 301

Query: 336 LGVPIPYSNLGLVNFNIV-----PDLRVPHLNKFQTLLLHSWQNAQQIYRYFFQYALENK 390
           LG+  P +N G +    +      +    H+N  Q L  +     Q    +F    LE  
Sbjct: 302 LGLAPPMNNFGKLPLGYLNVSKEYEAMAAHVNALQLLEQYVALQKQHKKGFFADILLEY- 360

Query: 391 RTFNVEQMDHLETEFILLTHRVQTVYNEVAFKSFVRDLNTN----LRDILGTCREIWVRF 446
              N E +   +   + L         +    S   D +        + +      + R 
Sbjct: 361 DVLNAEAIKRYKDTIVQL--------QKKREYSLCIDKSQVIMSQALEGIDYYHGYYRRV 412

Query: 447 DPTQMSQGLLFTFLPLFFIFLVVNNSRPADFP----HIFKAKEVFYVYLINLA---AGVF 499
                +   L     ++ +      S+          I +   V  V ++ +     G  
Sbjct: 413 LLLSTTSTFLGWMFYIYRLLSRNRASKVELVLERSNKIVRVSIVSSVIVLIICLLSQGTP 472

Query: 500 GYRYFKTFSFKTEEQGVIFFTAISSAVILA-FHTLRHWTSIATNWSAVKRFGHMPTRLLL 558
               F           +      +     +            + W  V         +  
Sbjct: 473 LDISFYLL-LPYFVW-LKALQPWNINFSSSPASAYSVPALQTSMWQLVLLIACAELMVFT 530

Query: 559 FGS-------MAVF-FSNSFVIQEAKILSYLLAAAILLLSHELLRLSARLD 601
           F           VF   NS+   + K   +    A++L+      L   + 
Sbjct: 531 FFERRLIALCFCVFACYNSWENFQWKSRDFFSWLALVLILSCFPLLPLSVG 581
>gb|AAF59557.2| (AC024812) Hypothetical protein Y54E10BR.1 [Caenorhabditis elegans]
          Length = 929

 Score =  377 bits (959), Expect = e-103
 Identities = 82/489 (16%), Positives = 166/489 (33%), Gaps = 77/489 (15%)

Query: 59  NEIFKDVNASS-NLCLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQ 117
           + +   +     N       ++ ++  D L+Y+      + +   PY + ++        
Sbjct: 27  SPLVHGIPPQFINSQEAPAKRIFIISADGLRYDTFNKYPDKS---PYLHSIM-------- 75

Query: 118 NPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ------I 171
           N         R+  PT +      +  G       +   +    +  D++ ++       
Sbjct: 76  NERKGIYGLSRSHIPTESRPGHVAIFAGITEDISAVAKGWKKNPVQFDSVFNRSSYSWMW 135

Query: 172 VKNDLPVVFLGDSTWTDLYPHRFKRSYSYPS--FDIFDLDSVDNEILKHLPKELE----- 224
              D+  +F       D  P+    SYS     F   D  ++D  + +H    LE     
Sbjct: 136 GSPDIVNLF-------DDLPNAESFSYSADEEDFASKDASNLDKWVFEHFENFLETAKTD 188

Query: 225 -------SKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDN----- 272
                   +   +   H LG+D  GH + PM  +    +  ++  I  V   +D      
Sbjct: 189 EALNDKMREQKSIFFLHLLGIDTNGHGNKPMSRQYIDNIKVVDSGIEKVQHLVDAFFGDH 248

Query: 273 DTTLLVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTL 332
            T  L   DHGMT  G HG  +DDE      A+    +      G     L QIDL P +
Sbjct: 249 KTAWLFTSDHGMTDWGSHGAGSDDEVLTPFVAWGAGVK-----QGGPKLDLNQIDLAPLI 303

Query: 333 ATILGVPIPYSNLGLVNFNIV------PDLRVPHLN----KFQTLLLHSWQNAQQIYRYF 382
           + ++G PIP +++G++   ++       + +    N    K Q + L + ++ +  +R F
Sbjct: 304 SALIGCPIPVNSMGILPVQMMNSKGSSYEFKAIEANFKQLKEQIIFLKNAKSRRLWFRQF 363

Query: 383 FQYALENKRTFNVEQMDHLETEFILLTHRVQTVYNEVAFKSFVRDLNTNLRDILGTCREI 442
            ++  +   +          T   L   R  +V       S   D    +++ +      
Sbjct: 364 EKFGDKAMESLRT-------TLAQLGRDRRFSVA-----TSLFADNAHLMKEAIVFYHRY 411

Query: 443 WVRFDPTQMSQGLLFTFLPLFFIFLVVNNSRPADFPHIFKAKEVFYVYLINLAAGVFGYR 502
             +     +S   +        +   ++NS   +   +        +++I LA  +    
Sbjct: 412 DRQMLGAAVSCSFVAWIA---LVVSFLHNSTSKENLSLLVPHH---LFIIPLACSLLFTA 465

Query: 503 YFKTFSFKT 511
           Y      +T
Sbjct: 466 YCSLSMTQT 474
>dbj|BAA82663.1| (AB030316) phosphatidylinositolglycan class N [Mus musculus]
          Length = 391

 Score =  377 bits (959), Expect = e-103
 Identities = 67/387 (17%), Positives = 134/387 (34%), Gaps = 62/387 (16%)

Query: 59  NEIFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRA-NATDPLPYENKLVVLQELLQQ 117
           + +   +        P   ++++ V D L+ +       +     P+   +++ +     
Sbjct: 26  SPLVHGMTPQFTPLPPPAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSW-- 83

Query: 118 NPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ------I 171
                         PT +      L  G       +   +    +  D++ ++       
Sbjct: 84  -------GVSHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSW 136

Query: 172 VKNDLPVVFL----GDSTWTDLYPHRFKRSYSYPS--FDIFDLDSVDNEI-----LKHLP 220
              D+  +F     GD  +T  Y  + +   ++ +   D +  D V +        + L 
Sbjct: 137 GSPDILPMFAKGASGDHVYTYSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLF 196

Query: 221 KELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM------DNDT 274
            ++ +++  V   H LG+D  GH H P   E    + ++++ ++ +V+        D  T
Sbjct: 197 TKV-NEEKVVFFLHLLGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKT 255

Query: 275 TLLVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSE------------- 321
             +   DHGMT  G HG     ET      +    +F  N S    +             
Sbjct: 256 AFIFTSDHGMTDWGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWK 315

Query: 322 --MLQQIDLVPTLATILGVPIPYSNLGLVNFNIVPDLRVPHLNKFQTLLLHS-WQNAQQI 378
              + Q D+ P +A+++GVP P +++G++            LN        S + NA QI
Sbjct: 316 RRDVNQADIAPLMASLIGVPFPLNSVGILPVGY--------LNNTGLFKAESMFTNAVQI 367

Query: 379 YRYFFQYALENKRTFNVEQMDHLETEF 405
              F     + K       +  L T F
Sbjct: 368 LEQFKVKMTQKKEAT----LPFLFTPF 390
>gb|AAF54657.1| (AE003692) CG6790 gene product [Drosophila melanogaster]
 gb|AAL28155.1| (AY060607) GH02677p [Drosophila melanogaster]
          Length = 858

 Score =  351 bits (893), Expect = 3e-95
 Identities = 73/521 (14%), Positives = 148/521 (28%), Gaps = 81/521 (15%)

Query: 65  VNASSNLC----LPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPD 120
           +     L      P   +++V +++ L+ +     ++      Y   +++ Q L      
Sbjct: 32  LEPQKTLLDMGVPPAADRLVVFLLEGLRADTLF--SDNCSGAVYIRDIILRQGL------ 83

Query: 121 HARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVF 180
              +   +   PT T      L  G  P    +      P  N D I ++ +  D     
Sbjct: 84  ---VGISKTSVPTLTRSAEVALFAGFNPMPSIL------PTSNFDTIFNRTLAFDK---- 130

Query: 181 LGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEIL-KHLPKELESKDWQVLVAHFLGVD 239
                        F R           L  +   +  +   ++L   +  V++ +   V 
Sbjct: 131 -----------GAFLRF--------SHLSDLRRRLTKRACLEKLWYANKLVMLVNLAEVG 171

Query: 240 HCGHKHGPMHEEMARKLGEMNEVIRSVVAAM-----DNDTTLLVMGDHGMTASGDHGGDT 294
                  P+      KL      IR     +     D+ T  L    HG+T  G HGG +
Sbjct: 172 GAS----PLDIGFQMKLHNTQRNIRDAYELIEATFNDSKTAYLYTSAHGLTYFGSHGGGS 227

Query: 295 DDETNALLFAYSKQHRFYGNDSGSDSEM----------LQQIDLVPTLATILGVPIPYSN 344
           D+E  A    +    +    +  SD  +          L QI L P ++ ++G+P P +N
Sbjct: 228 DEEREAPFLLWGAGVKHVTENITSDFVLNNGVGMQLHKLDQIQLAPLMSALIGLPPPVNN 287

Query: 345 LGLVNFNIVPDLRV-----PHLNKFQTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMD 399
           L ++    +   R       HLN  Q L              F ++  ++K   +++Q+ 
Sbjct: 288 LAILPQGYMKVSREYARKSVHLNALQLLTQAKAIIRLHERGVFHKWLPKDK-DLDLQQIA 346

Query: 400 HLETEFILLTHRVQTVYNEVAFKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGLLFTF 459
           + +   +     +      +   +            L      +           LL   
Sbjct: 347 YYQN-QMDHLLDMGWRSKALETSTLA---IKVALKSLKFYHNYYHIPLVVTTVLALLGWQ 402

Query: 460 LPLFFIFLVVNNSRPADFPHIFKAKEV-FYVYLINLAAGVFGYRYFKTFSFKTEEQGVIF 518
                +  +  N     +           ++  + L  G   +  +  F           
Sbjct: 403 FYQ--LVKLSQNVMAPHYMRRGYLTWCTIFLASLGLLLGEMVFLQWAPFMTVICLVVPFG 460

Query: 519 FTAISSAVILAFHTLRHWTSIATNWSAVKRFGHMPTRLLLF 559
              ++ A +               W      G     +   
Sbjct: 461 VWCMTLAALPQNGGWIFEPLKHLRW----IVGPAAVMIASI 497
>gb|AAF56048.1| (AE003740) CG4907 gene product [Drosophila melanogaster]
          Length = 919

 Score =  341 bits (868), Expect = 3e-92
 Identities = 83/506 (16%), Positives = 156/506 (30%), Gaps = 95/506 (18%)

Query: 59  NEIFKDVNASSNL----CLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQEL 114
           + I  D+   S L      P   +++V VVD L+ +     A+    +P   +L V Q  
Sbjct: 24  STILSDLEPLSTLRELGLEPPADRLVVFVVDGLRAQSV--LADHCSAVPDLRELFVEQ-- 79

Query: 115 LQQNPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ---- 170
                  A +   RA PPT T      +  G           +       D++ ++    
Sbjct: 80  -------ALVGISRACPPTVTRPGHIAIFGGFNEDP-AATLTW--NPSTFDSVFNRSRNA 129

Query: 171 --IVKNDLPVVFLGDSTWTDLYPHRFKRSYSYP-----SFDIFDLDSVDNEIL--KHLPK 221
              ++ ++  VF    T     P RF+             D +  D V N +   +++  
Sbjct: 130 IGWMQAEVAKVFTHLPTGGA--PLRFETFARSDISDRLRLDQWTFDKVRNFMTNEQNVQP 187

Query: 222 ELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM-----DNDTTL 276
            L      +   +   +D   H H P       KL      IR           D+ T  
Sbjct: 188 -LRDATSVIFFVYLADIDLAEHVHMPNSLNFREKLNYTQRGIRQTYELFESVFNDSRTAY 246

Query: 277 LVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSE-----------MLQQ 325
           L+  D+G++    HGG  +         +    +    + G +              L+Q
Sbjct: 247 LMTADYGISL---HGGGGERGVETPFILWGAGVKRSAPNPGQNFTAGENGPILPLYQLEQ 303

Query: 326 IDLVPTLATILGVPIPYSNL-----GLVNFNIVPDLRVPHLNKFQTLLLHSWQNAQQIYR 380
           I L P ++ ++G+P P +N+     G +N ++  +L+V HLN  Q L        +    
Sbjct: 304 IQLAPLMSALIGLPPPMNNMALMPLGFLNTSVQYELQVLHLNAMQLLAQARILIKRHEDG 363

Query: 381 YFFQ-----YALENKRTFNVEQM------DHLETEFILLTHRVQTVYNEVAFKSFVRDLN 429
             +       +L +    N  Q+           E + ++H++  +  E        +  
Sbjct: 364 ILYLLLPKFESLGSAEIENYPQIIKYLVAKEQYEEALKVSHKIAKLAQE------CME-- 415

Query: 430 TNLRDILGTCREIWVRFDPTQMSQGLLFTFLPLFFIFLVVNNSRPADFPHIFKAKE---- 485
                        +        +   L  F  L  + L   ++ P               
Sbjct: 416 --------YYHGYYHLPLLVATTASYLVWF-YLLLVRLARESNHPKSERK-GYLTWTTLM 465

Query: 486 ----VFYVYLINLAAGVFGYRYFKTF 507
                  +  + +   V     F   
Sbjct: 466 MCLGGLVLLELMILQRVPHLTAFYLL 491
>ref|NP_116023.1| (NM_032634) hypothetical protein MGC3079 [Homo sapiens]
 gb|AAH01030.1|AAH01030 (BC001030) Similar to phosphatidylinositol glycan, class O [Homo
            sapiens]
          Length = 454

 Score =  333 bits (847), Expect = 8e-90
 Identities = 67/236 (28%), Positives = 102/236 (42%), Gaps = 19/236 (8%)

Query: 840  VAVGAILLSVHSI-LRYRTATSFESCVQPTFTALVGWFLLAHFCFFATSHQTTLSQIEWR 898
             ++  +LL + +  +   T   F    Q    A+  W L+A   F++T HQ     I W 
Sbjct: 228  FSLSFLLLHLLAAGIPVTTPGPFTVPWQ----AVSAWALMATQTFYSTGHQPVFPAIHWH 283

Query: 899  AAFVGRTTGIGQSNLVSGALVILNTFCGPIFFFCMYSLLSTETFSLFALFPNLIRSCRSG 958
            AAFVG   G G    +   LV  NTF   + F     LL    F            C S 
Sbjct: 284  AAFVGFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFL-----------CESQ 332

Query: 959  GKVDASTSMSDLANEAVGFDMTRGELS---LYEYEDVFLGTGFKLATQFFMLQGLKIFCA 1015
            G         + A+  V  +     L    L +    F     +L  ++  + G++I   
Sbjct: 333  GLRKRQQPPGNEADARVRPEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGIQILAC 392

Query: 1016 MLACTIHCRHLMVWKIFAPRFIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRI 1071
             LA +I  RHLMVWK+FAP+FI+EA+   VS   L++G  LV+R+   V +  +++
Sbjct: 393  ALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDGAVSSWFRQL 448
 Score =  241 bits (611), Expect = 4e-62
 Identities = 89/233 (38%), Positives = 131/233 (56%), Gaps = 12/233 (5%)

Query: 223 LESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDH 282
           ++S +W+V   H LGVDHCGHKHGP H EMA+KL +M++VI+ +V  ++NDT L+V GDH
Sbjct: 1   MDSGEWEVSNQHVLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDH 60

Query: 283 GMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPY 342
           GMT +GDHGGD++ E +A LF YS    F  +    + E++ Q+ LVPTLA +LG+PIP+
Sbjct: 61  GMTTNGDHGGDSELEVSAALFLYSPTAVFP-STPPEEPEVIPQVSLVPTLALLLGLPIPF 119

Query: 343 SNLGLVNFNIVP--DLRVPHLNKF-QTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMD 399
            N+G V   +    +   PH +   Q   LH   NAQQ+ R+   Y+   +     +++ 
Sbjct: 120 GNIGEVMAELFSGGEDSQPHSSALAQASALH--LNAQQVSRFLHTYSAATQ-DLQAKELH 176

Query: 400 HLETEFILLTHRVQTVYN-----EVAFKSFVRDLNTNLRDILGTCREIWVRFD 447
            L+  F   +   Q +       E    + + +L   LR     C E W RF 
Sbjct: 177 QLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWARFS 229
>ref|XP_056208.1| (XM_056208) hypothetical protein MGC3079 [Homo sapiens]
 gb|AAH13987.1|AAH13987 (BC013987) Similar to hypothetical protein MGC3079 [Homo sapiens]
          Length = 454

 Score =  332 bits (843), Expect = 2e-89
 Identities = 67/236 (28%), Positives = 102/236 (42%), Gaps = 19/236 (8%)

Query: 840  VAVGAILLSVHSI-LRYRTATSFESCVQPTFTALVGWFLLAHFCFFATSHQTTLSQIEWR 898
             ++  +LL + +  +   T   F    Q    A+  W L+A   F++T HQ     I W 
Sbjct: 228  FSLSFLLLHLLAAGIPVTTPGPFTVPWQ----AVSAWALMATQTFYSTGHQPVFPAIHWH 283

Query: 899  AAFVGRTTGIGQSNLVSGALVILNTFCGPIFFFCMYSLLSTETFSLFALFPNLIRSCRSG 958
            AAFVG   G G    +   LV  NTF   + F     LL    F            C S 
Sbjct: 284  AAFVGFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFL-----------CESQ 332

Query: 959  GKVDASTSMSDLANEAVGFDMTRGELS---LYEYEDVFLGTGFKLATQFFMLQGLKIFCA 1015
            G         + A+  V  +     L    L +    F     +L  ++  + G++I   
Sbjct: 333  GLRKRQQPPGNEADARVRPEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGIQILAC 392

Query: 1016 MLACTIHCRHLMVWKIFAPRFIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRI 1071
             LA +I  RHLMVWK+FAP+FI+EA+   VS   L++G  LV+R+   V +  +++
Sbjct: 393  ALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDGAVSSWFRQL 448
 Score =  249 bits (631), Expect = 1e-64
 Identities = 92/233 (39%), Positives = 134/233 (57%), Gaps = 12/233 (5%)

Query: 223 LESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTLLVMGDH 282
           ++S +W VL+AHFLGVDHCGHKHGP H EMA+KL +M++VI+ +V  ++NDT L+V GDH
Sbjct: 1   MDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDH 60

Query: 283 GMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPY 342
           GMT +GDHGGD++ E +A LF YS    F  +    + E++ Q+ LVPTLA +LG+PIP+
Sbjct: 61  GMTTNGDHGGDSELEVSAALFLYSPTAVFP-STPPEEPEVIPQVSLVPTLALLLGLPIPF 119

Query: 343 SNLGLVNFNIVP--DLRVPHLNKF-QTLLLHSWQNAQQIYRYFFQYALENKRTFNVEQMD 399
            N+G V   +    +   PH +   Q   LH   NAQQ+ R+   Y+   +     +++ 
Sbjct: 120 GNIGEVMAELFSGGEDSQPHSSALAQASALH--LNAQQVSRFLHTYSAATQ-DLQAKELH 176

Query: 400 HLETEFILLTHRVQTVYN-----EVAFKSFVRDLNTNLRDILGTCREIWVRFD 447
            L+  F   +   Q +       E    + + +L   LR     C E W RF 
Sbjct: 177 QLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWARFS 229
>dbj|BAB29186.1| (AK014165) data source:MGD, source key:MGI:1351629,
           evidence:ISS~phosphatidylinositol glycan, class
           N~putative [Mus musculus]
          Length = 320

 Score =  313 bits (796), Expect = 8e-84
 Identities = 46/288 (15%), Positives = 99/288 (33%), Gaps = 34/288 (11%)

Query: 59  NEIFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRA-NATDPLPYENKLVVLQELLQQ 117
           + +   +        P   ++++ V D L+ +       +     P+   +++ +     
Sbjct: 26  SPLVHGMTPQFTPLPPPAKRLVLFVADGLRADTLYELDEDGNSRAPFIRNVIMHEGSW-- 83

Query: 118 NPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ------I 171
                         PT +      L  G       +   +    +  D++ ++       
Sbjct: 84  -------GVSHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVESDSLFNERKYTWSW 136

Query: 172 VKNDLPVVFL----GDSTWTDLYPHRFKRSYSYPS--FDIFDLDSVDNEI-----LKHLP 220
              D+  +F     GD  +T  Y  + +   ++ +   D +  D V +        + L 
Sbjct: 137 GSPDILPMFAKGASGDHVYTYSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLF 196

Query: 221 KELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM------DNDT 274
            ++ +++  V   H LG+D  GH H P   E    + ++++ ++ +V+        D  T
Sbjct: 197 TKV-NEEKVVFFLHLLGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKT 255

Query: 275 TLLVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSEM 322
             +   DHGMT  G HG     ET      +    +F  N S    + 
Sbjct: 256 AFIFTSDHGMTDWGSHGAGHPSETLTPFVTWGAGIKFPQNVSAQQYDD 303
>ref|NP_491097.1| (NM_058696) Y54E10BR.1.p [Caenorhabditis elegans]
          Length = 348

 Score =  313 bits (794), Expect = 1e-83
 Identities = 64/341 (18%), Positives = 121/341 (34%), Gaps = 55/341 (16%)

Query: 59  NEIFKDVNASS-NLCLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQ 117
           + +   +     N       ++ ++  D L+Y+      + +   PY + ++        
Sbjct: 27  SPLVHGIPPQFINSQEAPAKRIFIISADGLRYDTFNKYPDKS---PYLHSIM-------- 75

Query: 118 NPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQ------I 171
           N         R+  PT +      +  G       +   +    +  D++ ++       
Sbjct: 76  NERKGIYGLSRSHIPTESRPGHVAIFAGITEDISAVAKGWKKNPVQFDSVFNRSSYSWMW 135

Query: 172 VKNDLPVVFLGDSTWTDLYPHRFKRSYSYPS--FDIFDLDSVDNEILKHLPKELE----- 224
              D+  +F       D  P+    SYS     F   D  ++D  + +H    LE     
Sbjct: 136 GSPDIVNLF-------DDLPNAESFSYSADEEDFASKDASNLDKWVFEHFENFLETAKTD 188

Query: 225 -------SKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDN----- 272
                   +   +   H LG+D  GH + PM  +    +  ++  I  V   +D      
Sbjct: 189 EALNDKMREQKSIFFLHLLGIDTNGHGNKPMSRQYIDNIKVVDSGIEKVQHLVDAFFGDH 248

Query: 273 DTTLLVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTL 332
            T  L   DHGMT  G HG  +DDE      A+    +      G     L QIDL P +
Sbjct: 249 KTAWLFTSDHGMTDWGSHGAGSDDEVLTPFVAWGAGVK-----QGGPKLDLNQIDLAPLI 303

Query: 333 ATILGVPIPYSNLGLVNFNIV------PDLRVPHLNKFQTL 367
           + ++G PIP +++G++   ++       + +    N  Q +
Sbjct: 304 SALIGCPIPVNSMGILPVQMMNSKGSSYEFKAIEANFKQVI 344
>gb|AAK25979.1|AF360269_1 (AF360269) putative nucleotide pyrophosphatase [Arabidopsis
           thaliana]
          Length = 456

 Score =  246 bits (624), Expect = 1e-63
 Identities = 45/289 (15%), Positives = 92/289 (31%), Gaps = 40/289 (13%)

Query: 21  MLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEIFKDVNASSNLCLPQKSKVI 80
           MLF   FL +             +   NA     D   ++  +       L  P    V+
Sbjct: 1   MLFLSNFLSSSFLIFFIVATIAAAAAVNAGTKGLDSRPSKTRRPW-PFKKLNKPV---VL 56

Query: 81  VLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTLQRLK 140
           ++  D  ++ +                     +LL      A+        PT T     
Sbjct: 57  MISCDGFRFGYQFKTD------------TPNIDLLISGGTEAKHG-LIPVFPTMTFPNHY 103

Query: 141 GLTTGSLPTFIDIGSNFASPEI---------------NEDNIIDQIVKNDLPVVFLGDST 185
            + TG  P +  I  N  +  +                E   +  + +    V +   S 
Sbjct: 104 SIATGLYPAYHGIIMNKFTDPVTGEVFNKGLQPKWWLGEPLWVTAVNQGLKAVTYFWPS- 162

Query: 186 WTDLYPHRFKRSYSY-PSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDHCGHK 244
            +++    +     Y P F++        + +      LE +   +L+ +F   D  GH 
Sbjct: 163 -SEVLKSSWTCPEGYCPHFNLSVPLEERVDSVLSHFDHLEDEVPDLLMLYFDEPDQSGHN 221

Query: 245 HGPMHEEMARKLGEMNEVIRSVVAAMDND-----TTLLVMGDHGMTASG 288
           +GP    +   +  ++++I  V+  +          ++++GDHGM  + 
Sbjct: 222 YGPDDPRVTTAVSRVDKMIGRVIKGLKQREIFDEVHVILLGDHGMVTNC 270
>ref|NP_197227.1| (NM_121731) unknown protein [Arabidopsis thaliana]
 dbj|BAB10512.1| (AB005238) gene_id:MKP11.10~unknown protein [Arabidopsis thaliana]
          Length = 884

 Score =  238 bits (602), Expect = 4e-61
 Identities = 106/222 (47%), Positives = 146/222 (65%), Gaps = 9/222 (4%)

Query: 94  YRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDI 153
              ++++P P+ +KL +LQ L   N   A++ +  ADPPTT+LQRLKGLTTG LPTFIDI
Sbjct: 58  NHDSSSEPKPWMDKLTILQTLAFANDSSAKIFKAFADPPTTSLQRLKGLTTGGLPTFIDI 117

Query: 154 GSNFASPEINEDNIIDQIVKNDLPVVFLGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDN 213
           G++F +P I EDN I+Q+V N   +V +GD TWT L+P++F++SY +PSF++ DLD+VDN
Sbjct: 118 GNSFGAPAIVEDNFINQLVLNGKRLVMMGDDTWTQLFPNQFQKSYPFPSFNVKDLDTVDN 177

Query: 214 EILKHLPKELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDND 273
             ++HL   L   DW VL+AHFLGVDH GH  G     M  KL + N V+  V+  +++ 
Sbjct: 178 GCIEHLFPTLFKDDWDVLIAHFLGVDHAGHIFGVDSSPMINKLEQYNSVLEKVINILESQ 237

Query: 274 ---------TTLLVMGDHGMTASGDHGGDTDDETNALLFAYS 306
                    T L+VMGDHG T +GDHGG T +E    +FA S
Sbjct: 238 AGPGGLHENTMLIVMGDHGQTLNGDHGGGTAEEVETTMFAMS 279
 Score = 86.4 bits (212), Expect = 3e-15
 Identities = 15/50 (30%), Positives = 29/50 (58%)

Query: 997  GFKLATQFFMLQGLKIFCAMLACTIHCRHLMVWKIFAPRFIYEALATFVS 1046
             F++   F ++    +   +L  TI  RHLMVW +FAP+F+++ +   ++
Sbjct: 823  LFQMYMLFGVISATTVTATILCVTIQRRHLMVWGLFAPKFVFDVVDLILT 872
 Score = 39.7 bits (92), Expect = 0.30
 Identities = 26/121 (21%), Positives = 47/121 (38%), Gaps = 6/121 (4%)

Query: 823 VMIVLLEPRASIGLVVCVAVGAILLSVHSILRYRTATSFESCVQPTFTALVGWFLLAHFC 882
            +I+L   + S+  +V +  G  ++ +  + R   +       +     +V W LL+   
Sbjct: 693 PIILLSGKQGSMLALVYLVAGYCIMRLEGVERRTKSDGQSKFSKLNPLCVVQWSLLSICM 752

Query: 883 FFATSHQTTLSQIEWRAAFVGRTTGIGQSNLVSGALVILNTFCGPIFFFC-MYSLLSTET 941
           FFA+ H      + + AAFVG    +    +    L+ + TF   I        LL    
Sbjct: 753 FFASGHWCAFDGLRYGAAFVGFDEFV---LIRQAILLTIETFGFSIILSVFGLPLLI--P 807

Query: 942 F 942
           F
Sbjct: 808 F 808
 Score = 38.1 bits (88), Expect = 0.70
 Identities = 14/53 (26%), Positives = 25/53 (46%), Gaps = 1/53 (1%)

Query: 10 IWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNP-NAEYVLTDEVVNEI 61
          +    + +  +++F+RGFLL R      STC  +S +P  A      +  +E 
Sbjct: 13 LGFLLIHAIAILIFTRGFLLTRTELPFHSTCSDVSLSPCLASPRSNHDSSSEP 65
>sp|Q09782|YA93_SCHPO HYPOTHETICAL 85.7 KD PROTEIN C13G6.03 IN CHROMOSOME I
 pir||S62432 major facilitator protein homolog - fission yeast
           (Schizosaccharomyces pombe)
 pir||T37638 major facilitator protein homolog - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA91096.1| (Z54308) putative major facilitator superfamily protein
           [Schizosaccharomyces pombe]
          Length = 758

 Score =  235 bits (596), Expect = 2e-60
 Identities = 112/499 (22%), Positives = 185/499 (36%), Gaps = 78/499 (15%)

Query: 63  KDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHA 122
           K ++   +       +V+ ++VDAL+ +F   +++           +   + L  N  H 
Sbjct: 33  KAMSNQFSP-PAVIDQVVFVMVDALRADFVFSKSHN----------MPFTQSLLYNSTHG 81

Query: 123 RLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEIN-----EDNIIDQIVKNDLP 177
                 A  PT T+ RLK LTTG++P F+D+  N A  +       +D+ + Q+   +  
Sbjct: 82  IGFSAFARSPTVTMPRLKALTTGTIPGFLDVLLNIAESDTGSSIEAQDSWVYQLNSFNKK 141

Query: 178 VVFLGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKEL---ESKDWQVLVAH 234
           + F GD TW  L+P  F +     SF + D   VDN + ++    L    S  W  L+ H
Sbjct: 142 IEFYGDDTWLKLFPSAFSKFEGTTSFFVSDYTEVDNNVTRNFDHALPSSLSHSWDALILH 201

Query: 235 FLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM---DNDT----TLLVMGDHGMTAS 287
           +LGVDH GH +GP    +  KL E++ +I  +   +   D  T     +++ GDHGM   
Sbjct: 202 YLGVDHIGHLYGPSSPLLNIKLLEIDTIISRIYKYLQEYDEKTNTHSLIVLCGDHGMNEV 261

Query: 288 GDHGGDTDDETNALLFA---------YSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGV 338
           G+HGG +  ET A L            +K      ++  S  E ++Q+D+VPT+  +LG+
Sbjct: 262 GNHGGSSSGETTAALSLLFPSNELSHINKPILNMDDNPYSILERVEQVDVVPTICLLLGI 321

Query: 339 PIPYSNLGLV---NFNIVPDLRVPHLNKFQTLLLHSWQNAQQIYRYFFQYALENKRTFNV 395
           PIP  N+G V      +  D +   +     L   S                  K     
Sbjct: 322 PIPKGNMGKVLSPVTELWKDTKTAKMAALSNLFQLSLL----------------KNPSLT 365

Query: 396 EQMDHLETEFILLTHRVQTVYNEVAFKSFVRDLNTNLRDILGTCREIWVRFDPTQMSQGL 455
                L T+F         +  E      V   ++   D          R     ++  +
Sbjct: 366 --TSELSTQFQDSDLNDIRLALENLQSQMVAQSSSYSLD----------RML---VAISI 410

Query: 456 LFTFLPLFFIFLVVNNSRPADFPHIFKAK---EVFYVYLINLAAGVFGYRYFKTFSF--- 509
           L     L  I      +                + +         V  Y    + S    
Sbjct: 411 LGACSILSLILFRNLYNYKELLAFAPFVVQNIIIVFSSSFIEEEHVIWYFAAVSLSLLQL 470

Query: 510 ---KTEEQGVIFFTAISSA 525
              KT   G +    +S  
Sbjct: 471 LNPKTRLAGSLQLFCLSII 489
 Score = 36.9 bits (85), Expect = 1.9
 Identities = 11/37 (29%), Positives = 22/37 (58%)

Query: 1004 FFMLQGLKIFCAMLACTIHCRHLMVWKIFAPRFIYEA 1040
            F+++  + +    ++C I   HL VW +F+P+ +Y A
Sbjct: 701  FWIMSSISLTFLCISCFIMRHHLFVWSVFSPKLLYNA 737
>ref|NP_194698.1| (NM_119114) nucleotide pyrophosphatase - like protein [Arabidopsis
           thaliana]
 pir||T09932 nucleotide pyrophosphatase homolog T16L4.200 - Arabidopsis thaliana
 emb|CAB45329.1| (AL079344) nucleotide pyrophosphatase-like protein [Arabidopsis
           thaliana]
 emb|CAB79727.1| (AL161575) nucleotide pyrophosphatase-like protein [Arabidopsis
           thaliana]
          Length = 457

 Score =  235 bits (595), Expect = 3e-60
 Identities = 35/287 (12%), Positives = 90/287 (31%), Gaps = 43/287 (14%)

Query: 25  RGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEIFKDVNASSNLCLPQKSKVIVLVV 84
            GF  +     + S    +  +       +D   + + +       L  P    V+++  
Sbjct: 9   SGF--SGYILYKLSLTVLIVLSVAVTANGSDSPSSYVRRPQPP-KKLNKPV---VLLISC 62

Query: 85  DALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTLQRLKGLTT 144
           D  ++ +                     +LL      A+        PT T      + T
Sbjct: 63  DGFRFGYQFKTE------------TPNIDLLISRGTEAKTG-LIPVFPTMTFPNHYSIAT 109

Query: 145 GSLPTFIDIGSNFASPEI---------------NEDNIIDQIVKNDLPVVFLGDSTWTDL 189
           G  P    I  N  +  +                E   +  + +  +   +       D+
Sbjct: 110 GLYPASHGIIMNKFTDPVSGELFNRNLNPKWWLGEPLWVTAVNQGLMAATYFWP--GADV 167

Query: 190 YPHRFKRSYSYP--SFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDHCGHKHGP 247
           +   +     +    +++        + + +     E +    +  +F   D  GH++GP
Sbjct: 168 HKGSWNCPKGFCKAPYNVSVPLEERVDTILNYFDLPEREIPDFMALYFDEPDIQGHEYGP 227

Query: 248 MHEEMARKLGEMNEVIRSVVAAMDND-----TTLLVMGDHGMTASGD 289
               +   + +++++I  ++  ++         ++++GDHGM  + D
Sbjct: 228 DDPRVTEAVSKVDKMIGRIIMGLEKRKVFSDVHVILLGDHGMVTNCD 274
>dbj|BAB29052.1| (AK013913) data source:MGD, source key:MGI:1861452,
            evidence:ISS~phosphatidylinositol glycan, class
            O~putative [Mus musculus]
          Length = 171

 Score =  234 bits (592), Expect = 6e-60
 Identities = 50/170 (29%), Positives = 74/170 (43%), Gaps = 5/170 (2%)

Query: 902  VGRTTGIGQSNLVSGALVILNTFCGPIFFFCMYSLLSTETFSLFALFPNLIRSCRSGGKV 961
            VG   G G S  +   LV  NTF   + F     LL    F      P   R    G + 
Sbjct: 1    VGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKR-RQPLPGSES 59

Query: 962  DASTSMSDLANEAVGFDMTRGELSLYEYEDVFLGTGFKLATQFFMLQGLKIFCAMLACTI 1021
            +A     +           R    L +  + F     +L  ++  + G +I    LA +I
Sbjct: 60   EARVRPEEEEELQEPLMEMR----LRDAPNHFNAALLQLGLKYLFILGAQILACALAASI 115

Query: 1022 HCRHLMVWKIFAPRFIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRI 1071
              RHLMVWK+FAP+FI+EA+   VS   L++G  LV+R+   V +  K++
Sbjct: 116  LRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKL 165
>dbj|BAB20681.1| (AB032413) nucleotide pyrophosphatase-like protein [Spinacia
           oleracea]
          Length = 479

 Score =  232 bits (587), Expect = 3e-59
 Identities = 46/332 (13%), Positives = 94/332 (27%), Gaps = 58/332 (17%)

Query: 13  AFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEIFKDVNASSNLC 72
             LIS     F+        +    S     +              ++  K    + +L 
Sbjct: 42  VLLISLLSTTFTAVIAAVSFAFLFLSATSAAAG------------ESKTIKITTTARSLT 89

Query: 73  LPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPP 132
                 VI++  D  ++ +         P P  ++L+            A L       P
Sbjct: 90  KLTHPIVILISSDGFRFGYQFK-----APTPNIHRLINN-------GTSAELG-LIPVFP 136

Query: 133 TTTLQRLKGLTTGSLPTFIDIGSNFASPEI----------------NEDNIIDQIVKNDL 176
           T T      + TG  P    I +N+    I                 E            
Sbjct: 137 TLTFPNHYSIVTGLYPPHHGIINNYFVDPITGDKFSMASHEPKWWLGEPLWETVAKHGKK 196

Query: 177 PVVFLGDSTWTDLYPHRFKRSYSYP-SFDIFDLDSVDNEILKHLPKELESKDWQVLVAHF 235
              +      +++    +    +Y   ++         + +        ++    +  +F
Sbjct: 197 AATYFWP--GSEVIKPDWDCPSNYCIHYNGSIPFEERVDTVISYFDLPINEIPVFMTLYF 254

Query: 236 LGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTL-----LVMGDHGMTASGDH 290
              DH GH+ GP   E+ + +  ++++I  ++  ++           ++GDHGM  + D 
Sbjct: 255 EDPDHQGHQVGPDDPEITQAVINIDKMIGRLIKGLEKRGVFEDVNIFMVGDHGMVGTCDK 314

Query: 291 GGDTDDETN---------ALLFAYSKQHRFYG 313
                D+             L A S    F  
Sbjct: 315 KLIFLDDLEIPSNWVQSYTPLLAISPPPEFSP 346
>ref|NP_194699.1| (NM_119115) nucleotide pyrophosphatase -like protein [Arabidopsis
           thaliana]
 pir||T09933 nucleotide pyrophosphatase homolog T16L4.210 - Arabidopsis thaliana
 emb|CAB45330.1| (AL079344) nucleotide pyrophosphatase-like protein [Arabidopsis
           thaliana]
 emb|CAB79728.1| (AL161575) nucleotide pyrophosphatase-like protein [Arabidopsis
           thaliana]
          Length = 461

 Score =  228 bits (576), Expect = 5e-58
 Identities = 39/274 (14%), Positives = 85/274 (30%), Gaps = 40/274 (14%)

Query: 36  ETSTCRRLSTNPNAEYVLTDEVVNEIFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYR 95
                   +   NA     D   ++  +       L  P    V+++  D  ++ +    
Sbjct: 21  FIVATIAAAAAVNAGTKGLDSRPSKTRRPW-PFKKLNKPV---VLMISCDGFRFGYQFKT 76

Query: 96  ANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGS 155
                            +LL      A+        PT T      + TG  P +  I  
Sbjct: 77  D------------TPNIDLLISGGTEAKHG-LIPVFPTMTFPNHYSIATGLYPAYHGIIM 123

Query: 156 NFASPEI---------------NEDNIIDQIVKNDLPVVFLGDSTWTDLYPHRFKRSYSY 200
           N  +  +                E   +  + +    V +      +++    +     Y
Sbjct: 124 NKFTDPVTGEVFNKGLQPKWWLGEPLWVTAVNQGLKAVTYFWP--GSEVLKSSWTCPEGY 181

Query: 201 -PSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEM 259
            P F++        + +      LE +   +L+ +F   D  GH +GP    +   +  +
Sbjct: 182 CPHFNLSVPLEERVDSVLSHFDHLEDEVPDLLMLYFDEPDQSGHNYGPDDPRVTTAVSRV 241

Query: 260 NEVIRSVVAAMDND-----TTLLVMGDHGMTASG 288
           +++I  V+  +          ++++GDHGM  + 
Sbjct: 242 DKMIGRVIKGLKQREIFDEVHVILLGDHGMVTNC 275
>ref|NP_179839.1| (NM_127819) unknown protein [Arabidopsis thaliana]
 gb|AAF18652.1| (AC006592) unknown protein [Arabidopsis thaliana]
          Length = 897

 Score =  220 bits (556), Expect = 1e-55
 Identities = 114/382 (29%), Positives = 180/382 (46%), Gaps = 28/382 (7%)

Query: 7   FVLIWLAFLISSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNEIFKDVN 66
              +    L   G+ +F  GF   + +          S    +E        + +     
Sbjct: 13  IFTVAGILLQIIGLSIFVFGFFPVKPTL---------SGVSGSESYRDPFCDSSLIS--- 60

Query: 67  ASSNLCLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMR 126
             S L  P+K +++  V+D L  EF L   +   P     + +   + L  N D A    
Sbjct: 61  NESELHHPEKLRLLYQVIDGLPAEFVL-GKDGKPPEKVLKESMPYTQSLLANGD-AIGYH 118

Query: 127 FRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVFLGDSTW 186
            +A PPT T+ RLK + +G++  F+D+  NF +  + +DNI+ Q  +    +V LGD TW
Sbjct: 119 AKAAPPTVTMPRLKAMVSGAIGGFLDVAFNFNTQALLDDNILGQFFRIGWKMVMLGDETW 178

Query: 187 TDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDHCGHKHG 246
             L+P  F R     SF + D   VD  + +HLP EL S DW +L+ H+LG+DH GH  G
Sbjct: 179 LKLFPGLFMRHDGVSSFFVKDTVQVDRNVSRHLPDELNSDDWNLLILHYLGLDHVGHTGG 238

Query: 247 PMHEEMARKLGEMNEVIRSVV-------AAMDNDTTLLVMGDHGMTASGDHGGDTDDETN 299
                M  KL EM++++R++        +     T L+++ DHGMT +G+HGG + +ET+
Sbjct: 239 RNSPLMPAKLKEMDDIVRTMHLRAMMDRSHDQGQTLLIIVSDHGMTENGNHGGSSYEETD 298

Query: 300 ALLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGVPIPYSNLGLVNFN------IV 353
           +L+             S +++    Q+DL PTLA + GVPIP +N+G++           
Sbjct: 299 SLMLFIGLNSNISDYASATNNVAF-QVDLAPTLALLFGVPIPKNNVGVLVPGTLCSLRDF 357

Query: 354 PDLRVPHLNKFQTLLLHSWQNA 375
             LR   LN +Q L L   Q  
Sbjct: 358 EQLRALELNSWQLLRLMLAQIQ 379
>ref|NP_105837.1| (NC_002678) hypothetical protein [Mesorhizobium loti]
 dbj|BAB51623.1| (AP003005) hypothetical protein [Mesorhizobium loti]
          Length = 298

 Score =  220 bits (556), Expect = 1e-55
 Identities = 63/316 (19%), Positives = 119/316 (36%), Gaps = 54/316 (17%)

Query: 51  YVLTDEVVNEIFKDVNASSNLCLPQKSKVIVLVVDALKYEFGLYRANATDPLPYE--NKL 108
                 V     K         +  KSK++++++D                +PY    +L
Sbjct: 2   PETAHGVPVSTVKP----DGAAMALKSKLLLIILDG---------------VPYRNWRRL 42

Query: 109 VVLQELLQQNPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNII 168
           +   E   Q+   AR+ + R+  P+T+      + TG  P    I SN     + + +I 
Sbjct: 43  MGNLEGWVQSG-EARVWKMRSVLPSTSACCYASIHTGVSPQVHGILSNENRFRVGQPDIF 101

Query: 169 DQIVKNDLPVVFLGDSTWTDLY---------------------PHRFKRSYSYPSFDIFD 207
            ++ K       +  S W++ +                       RF     Y + +   
Sbjct: 102 SEVSKAGGKTGAVTHSYWSEFFRSYPFDLVEDMEYDEPGGPITHGRFHTMTGYNARNQMT 161

Query: 208 LDSVDNEILKHLPKELESKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVV 267
              VD      +  +    D+   + H   +D  GH+ G    EM   +  M+ ++ + +
Sbjct: 162 PSDVDLFATLTMLTKRHGIDYG--ILHTCTLDSMGHRFGNDCHEMDHAVYAMDGMLAAFL 219

Query: 268 AA-MDNDTTLLVMGDHGMTASGDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSE-MLQQ 325
              +     ++V  DHG T  G HGG  D+  +  L+       ++G   G +++ +L Q
Sbjct: 220 PGWIAAGYEVMVTADHGQTDRGHHGGHDDEMQDFALY-------YFGQGQGPETDTLLDQ 272

Query: 326 IDLVPTLATILGVPIP 341
           + L PT+ + LGVP+P
Sbjct: 273 LQLAPTVLSRLGVPVP 288
>gb|AAF56711.1| (AE003761) CG13978 gene product [Drosophila melanogaster]
          Length = 475

 Score =  218 bits (551), Expect = 4e-55
 Identities = 36/247 (14%), Positives = 73/247 (28%), Gaps = 26/247 (10%)

Query: 280 GDHGMTASGDHGGDTDDETNALLFAYSKQHRF------YGNDSGSDSEMLQQIDLVPTLA 333
             H       HG  +  E    LF +    +              +   + Q  L P ++
Sbjct: 209 AAHRFKPLRAHGSGSPLEVETPLFMWGAGVKRDEIDAEANFPEKPNISQVDQTQLAPLMS 268

Query: 334 TILGVPIPYSN-----LGLVNFNIVPDLRVPHLNKFQTLLLHSWQNAQQIYRYFFQYALE 388
           +++G+P P +N     +G +N +        HLN  Q L        +     F+++  +
Sbjct: 269 SLIGLPPPKNNLALMPVGYLNVSDEYQAVALHLNVLQLLSQAEILIGRHEKAIFYKWLPK 328

Query: 389 NKRTFNVEQMDHLETEF-ILLTHRVQTVYNEVAFKSFVRDLNTNLRDILGTCREIWVRFD 447
            K   ++  +D    +F IL +    T   E     F ++     +  L    + +    
Sbjct: 329 FKE-LHIGVIDQYAAQFDILKSEGNLTDAME-----FCKEYGKLAKKCLTYYNQYYQTPL 382

Query: 448 PTQMSQGLLFTFLPLFFIFLVVNNSRPA-------DFPHIFKAKEVFYVYLINLAAGVFG 500
               S   +  F     + L   + +             +  A     +  +     V  
Sbjct: 383 IVANSMSYMIWF-YCLLLQLTRLSKKEKMQRRGFMTVSTLILAMIGILLIAMMELQKVPH 441

Query: 501 YRYFKTF 507
              F   
Sbjct: 442 IAAFYLI 448
 Score =  163 bits (409), Expect = 2e-38
 Identities = 29/192 (15%), Positives = 62/192 (32%), Gaps = 31/192 (16%)

Query: 72  CLPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADP 131
             P   +++V V D L+     + AN    +P        +++ +Q     R+   R   
Sbjct: 41  LEPPADRLVVFVTDGLRA--ATFLANNGSDVPDL------KDIYRQQG---RIGISRTCA 89

Query: 132 PTTTLQRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVFLGDSTWTDLYP 191
           PT T      +  G          +      + D + ++       ++    S     + 
Sbjct: 90  PTMTRPGHIAIFAGFHEDPAASLMHLCYNPGDFDTVFNR----SRNMIGWAHSYIVGYFV 145

Query: 192 HRFK-----RSYSYPSFDI--------FDLDSVDNEILKHLPKELESKD--WQVLVAHFL 236
                    R  SY   D+        +  D V+N + +++    E ++    V   +  
Sbjct: 146 KLSHGGAPLRFDSYMERDLPEKLTCDKWAFDKVENFL-RNVDNVREWRNYKPAVFFVYLA 204

Query: 237 GVDHCGHKHGPM 248
            +D   H+  P+
Sbjct: 205 DMDIAAHRFKPL 216
>ref|NP_194697.1| (NM_119113) nucleotide pyrophosphatase - like protein [Arabidopsis
           thaliana]
 pir||T09931 nucleotide pyrophosphatase homolog T16L4.190 - Arabidopsis thaliana
 emb|CAB45328.1| (AL079344) nucleotide pyrophosphatase-like protein [Arabidopsis
           thaliana]
 emb|CAB79726.1| (AL161575) nucleotide pyrophosphatase-like protein [Arabidopsis
           thaliana]
          Length = 496

 Score =  214 bits (541), Expect = 6e-54
 Identities = 37/299 (12%), Positives = 92/299 (30%), Gaps = 45/299 (15%)

Query: 17  SSGVMLFSRGFLLARVSKTETSTCRRLSTNPNAEYVLTDEVVNE----IFKDVNASSNLC 72
            S   +F+   LL        ++        + + VL+   +++              L 
Sbjct: 47  ISSCFIFTS-LLLVTCIALSAASAFAFLFFSSQKPVLSLNQISKSPAFDRSVARPLKKLD 105

Query: 73  LPQKSKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPP 132
            P    V+++  D  ++ +          LP  ++L+            A         P
Sbjct: 106 KPV---VLLISSDGFRFGYQFKTK-----LPSIHRLIAN-------GTEAETG-LIPVFP 149

Query: 133 TTTLQRLKGLTTGSLPTFIDIGSNFASPE----------------INEDNIIDQIVKNDL 176
           T T      + TG  P +  I +N                     + E      + +   
Sbjct: 150 TLTFPNHYSIVTGLYPAYHGIINNHFVDPETGNVFTMASHEPEWWLGEPLWETVVNQGLK 209

Query: 177 PVVFLGDSTWTDLYPHRFKRSYSYP-SFDIFDLDSVDNEILKHLPKELESKDWQVLVAHF 235
              +      ++++   +        +++         + +        ++    +  +F
Sbjct: 210 AATYFWP--GSEVHKGSWNCPQGLCQNYNGSVPFDDRVDTILSYFDLPSNEIPSFMTLYF 267

Query: 236 LGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTTL-----LVMGDHGMTASGD 289
              DH GH+ GP   ++   +  ++ +I  ++  ++          +++GDHGM  + D
Sbjct: 268 EDPDHQGHQVGPDDPQITEAVVNIDRLIGRLIDGLEKRGIFEDVTMIMVGDHGMVGTCD 326
>ref|XP_082200.1| (XM_082200) CG6790 [Drosophila melanogaster]
          Length = 656

 Score =  212 bits (535), Expect = 3e-53
 Identities = 38/288 (13%), Positives = 80/288 (27%), Gaps = 27/288 (9%)

Query: 288 GDHGGDTDDETNALLFAYSKQHRFYGNDSGSDSEM----------LQQIDLVPTLATILG 337
           G HGG +D+E  A    +    +    +  SD  +          L QI L P ++ ++G
Sbjct: 19  GSHGGGSDEEREAPFLLWGAGVKHVTENITSDFVLNNGVGMQLHKLDQIQLAPLMSALIG 78

Query: 338 VPIPYSNLGLVNFNIVPDLRV-----PHLNKFQTLLLHSWQNAQQIYRYFFQYALENKRT 392
           +P P +NL ++    +   R       HLN  Q L              F ++  ++K  
Sbjct: 79  LPPPVNNLAILPQGYMKVSREYARKSVHLNALQLLTQAKAIIRLHERGVFHKWLPKDK-D 137

Query: 393 FNVEQMDHLETEFILLTHRVQTVYNEVAFKSFVRDLNTNLRDILGTCREIWVRFDPTQMS 452
            +++Q+ + +   +     +      +   +            L      +         
Sbjct: 138 LDLQQIAYYQN-QMDHLLDMGWRSKALETSTLA---IKVALKSLKFYHNYYHIPLVVTTV 193

Query: 453 QGLLFTFLPLFFIFLVVNNSRPADFPHIFKAKEV-FYVYLINLAAGVFGYRYFKTFSFKT 511
             LL        +  +  N     +           ++  + L  G   +  +  F    
Sbjct: 194 LALLGWQFYQ--LVKLSQNVMAPHYMRRGYLTWCTIFLASLGLLLGEMVFLQWAPFMTVI 251

Query: 512 EEQGVIFFTAISSAVILAFHTLRHWTSIATNWSAVKRFGHMPTRLLLF 559
                     ++ A +               W      G     +   
Sbjct: 252 CLVVPFGVWCMTLAALPQNGGWIFEPLKHLRW----IVGPAAVMIASI 295
>pir||T03293 nucleotide pyrophosphatase homolog - rice
 gb|AAA67067.1| (U25430) nucleotide pyrophosphatase precursor [Oryza sativa]
 gb|AAF65458.1|AF245483_1 (AF245483) OSE4 [Oryza sativa]
 dbj|BAB56086.1| (AP003244) nucleotide pyrophosphatase homolog [Oryza sativa]
          Length = 479

 Score =  200 bits (504), Expect = 1e-49
 Identities = 40/288 (13%), Positives = 82/288 (27%), Gaps = 40/288 (13%)

Query: 32  VSKTETSTCRRLSTNPNAEYVLTDEVVNEIFKDVNASSNLCLPQKSKVIVLVVDALKYEF 91
                 +     S                      A   L  P    VI++  D  ++ +
Sbjct: 48  HLLLTAALAVATSYLLLILPRTPLSAAPAPAAAARAQVKLEKPV---VILISSDGFRFGY 104

Query: 92  GLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTLQRLKGLTTGSLPTFI 151
                      P+ ++L+                      PT T      + TG  P+  
Sbjct: 105 Q-----HKAATPHIHRLIGNGTSAATG--------LVPIFPTLTFPNHYSIATGLYPSSH 151

Query: 152 DIGSNFASPEI----------------NEDNIIDQIVKNDLPVVFLGDSTWTDLYPHRFK 195
            I +N+    I                 E   +    +      +      +++    + 
Sbjct: 152 GIINNYFPDPISGDYFTMSSHEPKWWLGEPLWVTAADQGIQAATYFWP--GSEVKKGSWD 209

Query: 196 RSYSYP-SFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDHCGHKHGPMHEEMAR 254
               Y   ++         + +         +  Q L  +F   DH GH+ GP    +  
Sbjct: 210 CPDKYCRHYNGSVPFEERVDAILGYFDLPSDEMPQFLTLYFEDPDHQGHQVGPDDPAITE 269

Query: 255 KLGEMNEVIRSVVAAMDND-----TTLLVMGDHGMTASGDHGGDTDDE 297
            +  ++E+I  ++A ++         ++++GDHGM  + D      DE
Sbjct: 270 AVVRIDEMIGRLIAGLEERGVFEDVNVILVGDHGMVGTCDKKLVFLDE 317
>ref|NP_281901.1| (NC_002163) hypothetical protein Cj0729 [Campylobacter jejuni]
 pir||D81344 hypothetical protein Cj0729 [imported] - Campylobacter jejuni
           (strain NCTC 11168)
 emb|CAB73003.1| (AL139076) hypothetical protein Cj0729 [Campylobacter jejuni]
          Length = 269

 Score =  199 bits (503), Expect = 2e-49
 Identities = 53/281 (18%), Positives = 100/281 (34%), Gaps = 48/281 (17%)

Query: 77  SKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTL 136
           SK I++V+D L         +A+  + Y N L           +  +      + P+ + 
Sbjct: 3   SKAILVVLDGL------NCKSASINMGYLNALCK--------ENLGKFYSLECELPSMSR 48

Query: 137 QRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVFLGDSTWTDLYPHRFKR 196
              + L TG  P    I +N  S    + +I D   +  L           +LY     +
Sbjct: 49  PLYECLLTGVKPVLSGIINNKLSF-SKQTSIFDLCKEQGLKAGGAAYHWVFELY----NK 103

Query: 197 SYSYPSFDIFDLDSVDNEILKHL------PKELESK------------DWQVLVAHFLGV 238
               PS         D  +             L+S             +    + H + +
Sbjct: 104 KEFIPSLHR---HIEDENLTLPYGHFYYEDDYLDSHLFADGEHLRNKYNLDFTLIHSMNI 160

Query: 239 DHCGHKHGPMHEEMARKLGEMNEVIRSVVAA-MDNDTTLLVMGDHGMTASGDHGGDTDDE 297
           D  GHK G    E A K  +++ +I   +   ++    +++  DHGMT    HGG ++DE
Sbjct: 161 DDAGHKFGSHSIEYANKTKKVDILISEYLPTWLEQGINVIITSDHGMTEGKSHGGLSEDE 220

Query: 298 TNALLFAYSKQHRFYGNDSGSDSEMLQQIDLVPTLATILGV 338
                F +     +       ++  ++Q ++  ++  ILG+
Sbjct: 221 ILVPFFTFGSAFSY-------ENAKIKQDEICGSICEILGL 254
>ref|NP_251941.1| (NC_002516) hypothetical protein [Pseudomonas aeruginosa]
 pir||E83240 hypothetical protein PA3251 [imported] - Pseudomonas aeruginosa
           (strain PAO1)
 gb|AAG06639.1|AE004747_12 (AE004747) hypothetical protein [Pseudomonas aeruginosa]
          Length = 269

 Score =  186 bits (469), Expect = 1e-45
 Identities = 48/272 (17%), Positives = 83/272 (29%), Gaps = 34/272 (12%)

Query: 79  VIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTLQR 138
           VI++++D L +                + +  LQ L       +   R   + P+ +   
Sbjct: 5   VILVLLDGLNHSVA------------RDCMGHLQALCGAGRGQS--YRLECELPSLSRPL 50

Query: 139 LKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVFLGDSTWTDLYPH-----R 193
            + + TG  P    I  N       E ++        L          ++LY        
Sbjct: 51  YECILTGVPPIDSGILHNDVVRLSQEQSLFHYARAAGLSTAAAAYHWVSELYNRAPFDPA 110

Query: 194 FKRSYSYP-------SFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVDHCGHKHG 246
             R             F   D    D+ +               ++ H + +D  GH+HG
Sbjct: 111 RDRHTDDSALPIQRGHFYWSD-HYPDSHLFADAESLRRRHAPNFMLVHSMNIDDAGHRHG 169

Query: 247 PMHEEMARKLGEMNEVIRSVVAA-MDNDTTLLVMGDHGMTASGDHGGDTDDETNALLFAY 305
                        + ++   +   +     + +  DHGM     HGG  ++E    LF +
Sbjct: 170 LASPAYRNAARRADALLSEYLHQWLAAGYQVAITADHGMNEDRSHGGILEEEREVPLFVF 229

Query: 306 SKQHRFYGNDSGSDSEMLQQIDLVPTLATILG 337
                F  +D         Q DL  TL  ILG
Sbjct: 230 GDA--FSRDDLAQPL----QTDLCGTLCEILG 255
>dbj|BAA87261.1| (AB027957) Hypothetical protein [Schizosaccharomyces pombe]
          Length = 179

 Score =  181 bits (457), Expect = 4e-44
 Identities = 37/176 (21%), Positives = 71/176 (40%), Gaps = 25/176 (14%)

Query: 225 SKDWQVLVAHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAM------DNDTTLLV 278
            +D  V   H LG+D  GH   P   E    +  ++  I+ +V  M      D  ++ + 
Sbjct: 1   HRDKIVFFLHLLGIDTIGHNKHPDSVEYVENIQYIDGKIQELVDKMNNYYNNDGASSWVF 60

Query: 279 MGDHGMTASGDHGGDTDDETNALLFAYSKQH------RFYGNDSGSDS--------EMLQ 324
             DHGM+  G HG    D T   + A+          + YG+D  S            +Q
Sbjct: 61  TADHGMSDFGSHGDGNLDNTRTPIIAWGAGIQSPTHEKNYGHDEYSLPWNLTEIKRIDIQ 120

Query: 325 QIDLVPTLATILGVPIPYSNLGLVNFNIVP-----DLRVPHLNKFQTLLLHSWQNA 375
           Q D+   ++ ++G+  P +++G +  + +         V  +N  +    ++ ++A
Sbjct: 121 QADIAALMSYLVGLNFPVNSVGQIPLDYLDCSSRRKAEVALMNALEIGEQYNLKSA 176
>pir||T40657 probable phophodiesterase-nucleotide pyrophosp hatase precursor -
           fission yeast (Schizosaccharomyces pombe)
 emb|CAA22177.1| (AL034352) putative nucleotide pyrophosphatase precursor
           [Schizosaccharomyces pombe]
          Length = 485

 Score =  181 bits (456), Expect = 5e-44
 Identities = 37/236 (15%), Positives = 71/236 (29%), Gaps = 37/236 (15%)

Query: 77  SKVIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTL 136
           S VIV+ +D  + ++                      LL     +  +       P+ T 
Sbjct: 74  STVIVISLDGFRADYLY------------RGFTPN--LLSLAERNVHVPFLIPSFPSITF 119

Query: 137 QRLKGLTTGSLPTFIDIGSNFASPEINEDNIIDQIVKNDLPVVFL--GDSTW-------T 187
                + TG  P    I SN     +     ++ + + +    +   G+  W        
Sbjct: 120 PNHYTIVTGLYPESHGIVSNNFFDPVTGKQFVNSMPECNKDPTWWDKGEPIWVNAERNNV 179

Query: 188 DLYPHRFKRSYSYPSFDIFDLDSVDNEIL---------KHLPKELESKDWQVLVAHFLGV 238
               H +  +               N                   +    Q+L+A+   V
Sbjct: 180 RSAVHMWPGNEVENHGYRPTYSDGFNFDTTLREKKDRILEWLDLPDKDRPQLLLAYAPHV 239

Query: 239 DHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTT-----LLVMGDHGMTASGD 289
           D  GH  GP   E+   + E++ VI  ++  +          ++ + DHGM  + D
Sbjct: 240 DMVGHAFGPDSPELNIIIQEVDIVIGELIEGLKKRNIDKHVNIIFLSDHGMAPTSD 295
>ref|NP_006200.1| (NM_006209) ectonucleotide pyrophosphatase/phosphodiesterase 2
           (autotaxin); Phosphodiesterase I/nucleotide
           pyrophosphatase 2; autotaxin; phosphodiesterase
           I/nucleotide pyrophosphatase 2 (autotaxin) [Homo
           sapiens]
 gb|AAB00855.1| (L46720) autotaxin-t [Homo sapiens]
          Length = 863

 Score =  180 bits (454), Expect = 1e-43
 Identities = 39/232 (16%), Positives = 69/232 (28%), Gaps = 57/232 (24%)

Query: 79  VIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTLQR 138
           +I+  VD  +  +    +          K++   E L+    H+     R   PT T   
Sbjct: 166 LIIFSVDGFRASYMKKGS----------KVMPNIEKLRSCGTHSPY--MRPVYPTKTFPN 213

Query: 139 LKGLTTGSLPTFIDIGSNFASPEI-------------------NEDNIIDQIVKNDLPVV 179
           L  L TG  P    I  N     +                    +   I    +      
Sbjct: 214 LYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVKAGT 273

Query: 180 FLGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVD 239
           F     W+ + PH                       +       + +   V   +    D
Sbjct: 274 FF----WSVVIPHER-----------------RILTILQWLTLPDHERPSVYAFYSEQPD 312

Query: 240 HCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTT-----LLVMGDHGMTA 286
             GHK+GP   EM   L E+++++  ++  +          ++ +GDHGM  
Sbjct: 313 FSGHKYGPFGPEMTNPLREIDKIVGQLMDGLKQLKLHRCVNVIFVGDHGMED 364
>dbj|BAA08260.1| (D45421) phosphodiesterase I alpha [Homo sapiens]
          Length = 863

 Score =  180 bits (454), Expect = 1e-43
 Identities = 39/232 (16%), Positives = 69/232 (28%), Gaps = 57/232 (24%)

Query: 79  VIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTLQR 138
           +I+  VD  +  +    +          K++   E L+    H+     R   PT T   
Sbjct: 166 LIIFSVDGFRASYMKKGS----------KVMPNIEKLRSCGTHSPY--MRPVYPTKTFPN 213

Query: 139 LKGLTTGSLPTFIDIGSNFASPEI-------------------NEDNIIDQIVKNDLPVV 179
           L  L TG  P    I  N     +                    +   I    +      
Sbjct: 214 LYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVKAGT 273

Query: 180 FLGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVD 239
           F     W+ + PH                       +       + +   V   +    D
Sbjct: 274 FF----WSVVIPHER-----------------RILTILQWLTLPDHERPSVYAFYSEQPD 312

Query: 240 HCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTT-----LLVMGDHGMTA 286
             GHK+GP   EM   L E+++++  ++  +          ++ +GDHGM  
Sbjct: 313 FSGHKYGPFGPEMTNPLREIDKIVGQLMDGLKQLKLHRCVNVIFVGDHGMED 364
>ref|XP_080427.1| (XM_080427) similar to CG12263 gene product (D. melanogaster)
            [Drosophila melanogaster]
          Length = 111

 Score =  178 bits (449), Expect = 3e-43
 Identities = 111/111 (100%), Positives = 111/111 (100%)

Query: 967  MSDLANEAVGFDMTRGELSLYEYEDVFLGTGFKLATQFFMLQGLKIFCAMLACTIHCRHL 1026
            MSDLANEAVGFDMTRGELSLYEYEDVFLGTGFKLATQFFMLQGLKIFCAMLACTIHCRHL
Sbjct: 1    MSDLANEAVGFDMTRGELSLYEYEDVFLGTGFKLATQFFMLQGLKIFCAMLACTIHCRHL 60

Query: 1027 MVWKIFAPRFIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRINKAKVH 1077
            MVWKIFAPRFIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRINKAKVH
Sbjct: 61   MVWKIFAPRFIYEALATFVSLPALIVGYLLVLRIHRGVDTLIKRINKAKVH 111
>gb|AAH03264.1|AAH03264 (BC003264) ectonucleotide pyrophosphatase/phosphodiesterase 2 [Mus
           musculus]
          Length = 862

 Score =  178 bits (449), Expect = 3e-43
 Identities = 40/232 (17%), Positives = 68/232 (29%), Gaps = 57/232 (24%)

Query: 79  VIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTLQR 138
           +I+  VD  +  +    +          K++   E L+    HA     R   PT T   
Sbjct: 165 LIIFSVDGFRASYMKKGS----------KVMPNIEKLRSCGTHAPY--MRPVYPTKTFPN 212

Query: 139 LKGLTTGSLPTFIDIGSNFASPEI-------------------NEDNIIDQIVKNDLPVV 179
           L  L TG  P    I  N     +                    +   I    +      
Sbjct: 213 LYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGT 272

Query: 180 FLGDSTWTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLVAHFLGVD 239
           F     W+   PH                       +       +++   V   +    D
Sbjct: 273 FF----WSVSIPHER-----------------RILTILQWLSLPDNERPSVYAFYSEQPD 311

Query: 240 HCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTT-----LLVMGDHGMTA 286
             GHK+GP   EM   L E+++ +  ++  +          ++ +GDHGM  
Sbjct: 312 FSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRCVNVIFVGDHGMED 363
>pir||T33724 hypothetical protein - Zymomonas mobilis
 gb|AAC70363.1| (AF086791) unknown [Zymomonas mobilis]
          Length = 429

 Score =  176 bits (442), Expect = 2e-42
 Identities = 45/240 (18%), Positives = 84/240 (34%), Gaps = 49/240 (20%)

Query: 79  VIVLVVDALKYEFGLYRANATDPLPYENKLVVLQELLQQNPDHARLMRFRADPPTTTLQR 138
           +I++ +D  + ++                L      L +N  +A++       P+ T   
Sbjct: 50  LILISIDGFRADYIK------------RGLTPNLVSLAENGSYAKV--MHPSFPSITFPN 95

Query: 139 LKGLTTGSLPTFIDI-GSNFASPEINEDNIID------------------QIVKNDL--- 176
              L TG  P    I G+N     I  D+                       V  +    
Sbjct: 96  HYTLVTGLYPDHHGIVGNNMDDAHITPDSHFKMSDHQAATDRRWWDEGEPLWVTAEKQGV 155

Query: 177 --PVVFLGDST--WTDLYPHRFKRSYSYPSFDIFDLDSVDNEILKHLPKELESKDWQVLV 232
               +F   S      + P  +++  S+  F     D V + +        + K  Q + 
Sbjct: 156 VSATMFWPGSEADIEGVRPEMWRQFDSHVPFSERV-DQVFSWLA-----YPKKKRPQFIT 209

Query: 233 AHFLGVDHCGHKHGPMHEEMARKLGEMNEVIRSVVAAMDNDTT---LLVMGDHGMTASGD 289
            +F  VDH GH +GP  +E+   L ++++ I  +V  +        L+++ DHGM A+  
Sbjct: 210 LYFENVDHAGHLYGPDSQEVNDNLVKIDQTIGQLVQGLKQRGIKANLVIVSDHGMAATSS 269
CPU time:   284.97 user secs.	    3.18 sys. secs	  288.15 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.315    0.166    0.471 

Gapped
Lambda     K      H
   0.270   0.0583    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 652996244
Number of Sequences: 887402
Number of extensions: 31726087
Number of successful extensions: 154138
Number of sequences better than 10.0: 523
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 395
Number of HSP's that attempted gapping in prelim test: 152940
Number of HSP's gapped (non-prelim): 968
length of query: 1077
length of database: 277,845,442
effective HSP length: 57
effective length of query: 1020
effective length of database: 227,263,528
effective search space: 231808798560
effective search space used: 231808798560
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.8 bits)
S2: 78 (34.5 bits)