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GPI Anchor Biosynthesis Report: orf6.7880.prot (PIG-M family, Candida albicans)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (398 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 18 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase ... 468 e-130
dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:19148... 467 e-130
gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mu... 467 e-130
ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosylt... 466 e-130
ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans] ... 455 e-127
emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [S... 450 e-125
dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei] 427 e-118
ref|NP_012547.1| (NC_001142) Protein required for cell viability... 339 5e-92
ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster] >g... 298 1e-79
emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Ara... 270 3e-71
dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharom... 165 1e-39
ref|NP_346994.1| (NC_003030) Uncharacterized membrane protein [C... 104 4e-21
Sequences with E-value WORSE than threshold

ref|NP_536724.1| (NM_080476) CDC91 cell division cycle 91-like 1... 36 0.81
ref|NP_563912.1| (NM_101143) expressed protein [Arabidopsis thal... 36 1.4
ref|NP_488389.1| (NC_003272) unknown protein [Nostoc sp. PCC 712... 35 1.8
ref|XP_079016.1| (XM_079016) CG13089 [Drosophila melanogaster] >... 33 7.6
Alignments
>ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase [Rattus norvegicus]
 dbj|BAB18566.1| (AB028126) mannosyltransferase [Rattus norvegicus]
          Length = 423

 Score =  468 bits (1192), Expect = e-130
 Identities = 144/398 (36%), Positives = 225/398 (56%), Gaps = 25/398 (6%)

Query: 10  FSILLRFGFFFFGLYQDEYMPVKYTDIDYLVFNDASKFVYQGLSPYLRETYRYTPILAIL 69
            + L R    F+G++QD  + V+YTDIDY VF DA++FV +G SPYLR TYRYTP+L+ L
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 70  LIPDNFGKYWYHFGKLLFMVSDVITGLIILKLLSKQQQLSEKKKMILSSIWLLNPMVITI 129
           L P+ +      FGK LF+  D++T   +L  L   + L  ++       WLLNP+ + +
Sbjct: 84  LTPNVYLSEL--FGKFLFISCDLLTAF-LLYRLLLLKGLGRRQACGYCVFWLLNPLPMAV 140

Query: 130 STRGSAESVLTVMIMLSLYYLLDKDNVILSAIWLGLSIHFKIYPIIYLPSILYYLSSQET 189
           S+RG+A+S++  +++ +LY L++K  +  +A++ G ++H K+YP+ Y+  I  +L  +  
Sbjct: 141 SSRGNADSIVASLVLTTLY-LIEKRLIACAAVFYGFAVHMKMYPVTYILPIALHLRPERD 199

Query: 190 ----------PFLASVPGI--NLVNAKNLKYIIITLTTLAVVNYLMFLKYGWEFIDNSYL 237
                      F A +      L +   L ++ I   T   +++  + KYGWEF++++YL
Sbjct: 200 SDEGLRLARYSFQARLYDFLKRLCSWAVLLFVAIAGLTFLALSFGFYYKYGWEFLEHTYL 259

Query: 238 YHVTRLDHRHNFSVYNMVLYYKSALLEDSNGFDIEKIAFVPQLLLSAVIIPLIFAKEDLI 297
           YH+TR D RHNFS Y  +LY  +   E    F +   AF+PQ +L +      +   DL+
Sbjct: 260 YHLTRRDIRHNFSPYFYMLYLTA---ESKWSFTLGIAAFLPQFILLSAASFAYY--RDLV 314

Query: 298 SSLFIQTFVFVAFNKVITSQYFIWFLIFLPHFLSKTKLLTTDKITGISCLLLWIISQATW 357
              F+ T +FV FNKV TSQYF+W+L  LP  +   ++     +     L+LW I QA W
Sbjct: 315 FCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVV---LLMLWFIGQALW 371

Query: 358 LYFAYKLEFLGENTFDNGLMYSSVFFFLSNCWCTMKFI 395
           L  AY LEF G+NTF   +  + +FF L NC   ++ I
Sbjct: 372 LAPAYVLEFQGKNTF-LFIWLAGLFFLLINCSILIQII 408
>dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:1914806,
           evidence:ISS~phosphatidylinositol glycan, class
           M~putative [Mus musculus]
          Length = 423

 Score =  467 bits (1190), Expect = e-130
 Identities = 141/398 (35%), Positives = 223/398 (55%), Gaps = 25/398 (6%)

Query: 10  FSILLRFGFFFFGLYQDEYMPVKYTDIDYLVFNDASKFVYQGLSPYLRETYRYTPILAIL 69
            + L R    F+G++QD  + V+YTDIDY VF DA++FV +G SPYLR TYRYTP+L+ L
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 70  LIPDNFGKYWYHFGKLLFMVSDVITGLIILKLLSKQQQLSEKKKMILSSIWLLNPMVITI 129
           L P+ +      FGK LF+  D++T   +L  L   + L  ++       WLLNP+ + +
Sbjct: 84  LTPNVYLSEL--FGKFLFISCDLLTAF-LLYRLLLLKGLGRRQACGYCVFWLLNPLPMAV 140

Query: 130 STRGSAESVLTVMIMLSLYYLLDKDNVILSAIWLGLSIHFKIYPIIYLPSILYYLSSQET 189
           S+RG+A+S++  +++ +LY+ ++K  +  +A++ G ++H K+YP+ Y+  I  +L  +  
Sbjct: 141 SSRGNADSIVASLVLSTLYF-IEKRLIACAAVFYGFAVHMKMYPVTYILPIALHLRPERD 199

Query: 190 ----------PFLASVPGI--NLVNAKNLKYIIITLTTLAVVNYLMFLKYGWEFIDNSYL 237
                      F A +      L +   L ++ +   T   +++  + KYGWEF++++Y 
Sbjct: 200 DDERLRQARFSFQARLYDFLRRLCSWAVLLFVAVAGLTFVALSFGFYYKYGWEFLEHTYF 259

Query: 238 YHVTRLDHRHNFSVYNMVLYYKSALLEDSNGFDIEKIAFVPQLLLSAVIIPLIFAKEDLI 297
           YH+TR D RHNFS Y  +LY  +   E    F +   AF+PQ +L +      +   DL+
Sbjct: 260 YHLTRRDIRHNFSPYFYMLYLTA---ESKWSFTLGIAAFLPQFILLSAASFAYY--RDLV 314

Query: 298 SSLFIQTFVFVAFNKVITSQYFIWFLIFLPHFLSKTKLLTTDKITGISCLLLWIISQATW 357
              F+ T +FV FNKV TSQYF+W+L  LP  +   ++     +     LL W I QA W
Sbjct: 315 FCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVV---LLLFWFIGQALW 371

Query: 358 LYFAYKLEFLGENTFDNGLMYSSVFFFLSNCWCTMKFI 395
           L  AY LEF G+NTF   +  + +FF L NC   ++ I
Sbjct: 372 LAPAYVLEFQGKNTF-LFIWLAGLFFLLINCSILIQII 408
>gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mus musculus]
          Length = 423

 Score =  467 bits (1190), Expect = e-130
 Identities = 141/398 (35%), Positives = 223/398 (55%), Gaps = 25/398 (6%)

Query: 10  FSILLRFGFFFFGLYQDEYMPVKYTDIDYLVFNDASKFVYQGLSPYLRETYRYTPILAIL 69
            + L R    F+G++QD  + V+YTDIDY VF DA++FV +G SPYLR TYRYTP+L+ L
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 70  LIPDNFGKYWYHFGKLLFMVSDVITGLIILKLLSKQQQLSEKKKMILSSIWLLNPMVITI 129
           L P+ +      FGK LF+  D++T   +L  L   + L  ++       WLLNP+ + +
Sbjct: 84  LTPNVYLSEL--FGKFLFISCDLLTAF-LLYRLLLLKGLGRRQACGYCVFWLLNPLPMAV 140

Query: 130 STRGSAESVLTVMIMLSLYYLLDKDNVILSAIWLGLSIHFKIYPIIYLPSILYYLSSQET 189
           S+RG+A+S++  +++ +LY+ ++K  +  +A++ G ++H K+YP+ Y+  I  +L  +  
Sbjct: 141 SSRGNADSIVASLVLSTLYF-IEKRLIACAAVFYGFAVHMKMYPVTYILPIALHLRPERD 199

Query: 190 ----------PFLASVPGI--NLVNAKNLKYIIITLTTLAVVNYLMFLKYGWEFIDNSYL 237
                      F A +      L +   L ++ +   T   +++  + KYGWEF++++Y 
Sbjct: 200 DDERLRHARFSFQARLYDFLRRLCSWAVLLFVAVAGLTFVALSFGFYYKYGWEFLEHTYF 259

Query: 238 YHVTRLDHRHNFSVYNMVLYYKSALLEDSNGFDIEKIAFVPQLLLSAVIIPLIFAKEDLI 297
           YH+TR D RHNFS Y  +LY  +   E    F +   AF+PQ +L +      +   DL+
Sbjct: 260 YHLTRRDIRHNFSPYFYMLYLTA---ESKWSFTLGIAAFLPQFILLSAASFAYY--RDLV 314

Query: 298 SSLFIQTFVFVAFNKVITSQYFIWFLIFLPHFLSKTKLLTTDKITGISCLLLWIISQATW 357
              F+ T +FV FNKV TSQYF+W+L  LP  +   ++     +     LL W I QA W
Sbjct: 315 FCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVV---LLLFWFIGQALW 371

Query: 358 LYFAYKLEFLGENTFDNGLMYSSVFFFLSNCWCTMKFI 395
           L  AY LEF G+NTF   +  + +FF L NC   ++ I
Sbjct: 372 LAPAYVLEFQGKNTF-LFIWLAGLFFLLINCSILIQII 408
>ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosyltransferase [Homo
           sapiens]
 dbj|BAB18567.1| (AB028127) mannosyltransferase [Homo sapiens]
 gb|AAH19865.1|AAH19865 (BC019865) Unknown (protein for MGC:29896) [Homo sapiens]
          Length = 423

 Score =  466 bits (1189), Expect = e-130
 Identities = 145/398 (36%), Positives = 224/398 (55%), Gaps = 25/398 (6%)

Query: 10  FSILLRFGFFFFGLYQDEYMPVKYTDIDYLVFNDASKFVYQGLSPYLRETYRYTPILAIL 69
            + L R    F+G++QD  + V+YTDIDY VF DA++FV +G SPYLR TYRYTP+L  L
Sbjct: 24  VAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLGWL 83

Query: 70  LIPDNFGKYWYHFGKLLFMVSDVITGLIILKLLSKQQQLSEKKKMILSSIWLLNPMVITI 129
           L P+ +      FGK LF+  D++T   +L  L   + L  ++       WLLNP+ + +
Sbjct: 84  LTPNIYLSEL--FGKFLFISCDLLTAF-LLYRLLLLKGLGRRQACGYCVFWLLNPLPMAV 140

Query: 130 STRGSAESVLTVMIMLSLYYLLDKDNVILSAIWLGLSIHFKIYPIIYLPSILYYLSSQET 189
           S+RG+A+S++  ++++ LY L+ K  V  +A++ G ++H KIYP+ Y+  I  +L     
Sbjct: 141 SSRGNADSIVASLVLMVLY-LIKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPDRD 199

Query: 190 ----------PFLASVPGI--NLVNAKNLKYIIITLTTLAVVNYLMFLKYGWEFIDNSYL 237
                      F A +  +   L N   L ++ +   T   +++  + +YGWEF++++Y 
Sbjct: 200 NDKSLRQFRYTFQACLYELLKRLCNRAVLLFVAVAGLTFFALSFGFYYEYGWEFLEHTYF 259

Query: 238 YHVTRLDHRHNFSVYNMVLYYKSALLEDSNGFDIEKIAFVPQLLLSAVIIPLIFAKEDLI 297
           YH+TR D RHNFS Y  +LY  +   E    F +   AF+PQL+L + +    +   DL+
Sbjct: 260 YHLTRRDIRHNFSPYFYMLYLTA---ESKWSFSLGIAAFLPQLILLSAVSFAYY--RDLV 314

Query: 298 SSLFIQTFVFVAFNKVITSQYFIWFLIFLPHFLSKTKLLTTDKITGISCLLLWIISQATW 357
              F+ T +FV FNKV TSQYF+W+L  LP  +   ++     +     L+LW I QA W
Sbjct: 315 FCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVV---LLMLWFIGQAMW 371

Query: 358 LYFAYKLEFLGENTFDNGLMYSSVFFFLSNCWCTMKFI 395
           L  AY LEF G+NTF   +  + +FF L NC   ++ I
Sbjct: 372 LAPAYVLEFQGKNTF-LFIWLAGLFFLLINCSILIQII 408
>ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans]
 pir||T18769 hypothetical protein B0491.1 - Caenorhabditis elegans
 emb|CAA90090.1| (Z49907) B0491.1 [Caenorhabditis elegans]
          Length = 417

 Score =  455 bits (1160), Expect = e-127
 Identities = 134/400 (33%), Positives = 210/400 (52%), Gaps = 21/400 (5%)

Query: 6   YLITFSILLRFGFFFFGLYQDEYMPVKYTDIDYLVFNDASKFVYQGLSPYLRETYRYTPI 65
            ++  + + R    F+    D    V +TDIDY VF+DA+K V  G SP+ R TYRYTP 
Sbjct: 17  KILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPA 76

Query: 66  LAILLIPDNFGKYWYHFGKLLFMVSDVITGLIILKLLSK---------QQQLSEKKKMIL 116
           LA +L+P     ++  FGK+LF + D++  ++  K++ K         ++++ + + M +
Sbjct: 77  LAWILLP---VVHFPDFGKILFCIFDILVAILYFKIMEKDLNETKSETREEMKDDQTMNV 133

Query: 117 SSIWLLNPMVITISTRGSAESVLTVMIMLSLYYLLDKDNVILSAIWLGLSIHFKIYPIIY 176
              WL NP+   IS RG+AES++  +++L++  L        + +   L+I  KIYP+IY
Sbjct: 134 VIYWLANPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIYPLIY 193

Query: 177 LPSILYYLSS-QETPFLASVPGINLVNAKNLKYIIITLTTLAVVNYLMFLKYGWEFIDNS 235
           LPS+   LS+  E   + +     + N K   Y+++TLT+ A V    F  YG  F+D  
Sbjct: 194 LPSVFLSLSTIGEQSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLFFFQIYGQLFLDEY 253

Query: 236 YLYHVTRLDHRHNFSVYNMVLYYKSALLEDSNGFDIEKIAFVPQLLLSAVIIPLIFAKED 295
            +YHV R D  HNFS Y  +LY   A    S    +   AF+PQ++L  ++       +D
Sbjct: 254 LIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLG--AFIPQIVL--IVFFAFKHYDD 309

Query: 296 LISSLFIQTFVFVAFNKVITSQYFIWFLIFLPHFLSKTKLLTTDKITGISCLLLWIISQA 355
           L    FI TF FV +NKV TSQYF+W+++ LP    K   +   +   +S +  W  +Q 
Sbjct: 310 LPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLLAHK---IMMSRQLALSLMAAWFATQG 366

Query: 356 TWLYFAYKLEFLGENTFDNGLMYSSVFFFLSNCWCTMKFI 395
            WL  AY  EF G NTF   +  +S  F ++N +   + I
Sbjct: 367 IWLLAAYLFEFQGWNTFFL-MFLASCLFLIANSFILKQII 405
>emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [Schizosaccharomyces
           pombe]
          Length = 815

 Score =  450 bits (1147), Expect = e-125
 Identities = 170/392 (43%), Positives = 256/392 (64%), Gaps = 14/392 (3%)

Query: 7   LITFSILLRFGFFFFGLYQDEYMPVKYTDIDYLVFNDASKFVYQGLSPYLRETYRYTPIL 66
           L+   +L+R     +G++ D    +K+TDIDY VF+DASK+V  G+SPY+R+TYRYTP+L
Sbjct: 438 LLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSPYMRDTYRYTPML 497

Query: 67  AILLIPDNFGKYWYHFGKLLFMVSDVITGLIILKLLSKQQQLSEKKKMILSSIWLLNPMV 126
           AILL+P  +G  +  +GK LF +SD+I G +++KLLS+  ++S K+ +I SS W+LNP V
Sbjct: 498 AILLLPTQYG--FPSWGKYLFSISDLIAGWLMIKLLSR--RISYKRSLIYSSFWILNPFV 553

Query: 127 ITISTRGSAESVLTVMIMLSLYYLLDKDNVILSAIWLGLSIHFKIYPIIYLPSILYYLSS 186
             ISTRG+ E++L ++ + +L YL++K +V L+++ LG S+HFKIYP +Y  + L Y S 
Sbjct: 554 AIISTRGNCEAILGILSI-ALLYLIEKKSVWLASLILGFSVHFKIYPFMYGIAFLVYFSK 612

Query: 187 QETPFLASVPGINLVNAKNLKYIIITLTTLAVVNYLMFLKYGWEFIDNSYLYHVTRLDHR 246
            +         ++L++   LK ++ +L    + N LM+  YG  F++++YLYH  R DHR
Sbjct: 613 PKKGSTFMEKFLSLLSINQLKIVVGSLFMFTICNLLMYYLYGSPFLEHTYLYHFGRTDHR 672

Query: 247 HNFSVYNMVLYYKSALLEDSNGFDIEKIAFVPQLLLSAVIIPLIFAKEDLISSLFIQTFV 306
           HNFS++++ LYY+S+    ++       AF+PQL L  ++IPL+F K++L  +LF QTF 
Sbjct: 673 HNFSLHHLNLYYESSFGAKASSL----FAFLPQLSL-CMLIPLVFGKKNLPGTLFAQTFA 727

Query: 307 FVAFNKVITSQYFIWFLIFLPHFLSKTKLLTTDKITGISCLLLWIISQATWLYFAYKLEF 366
           FV FNKV TSQYF+W+L+FLP  L  +KLL+     G+ CL LWII Q  WL  AY LE 
Sbjct: 728 FVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSK---KGLICLSLWIIGQLLWLISAYNLEM 784

Query: 367 LGENTFDNGLMYSSVFFFLSNCWCTMKFIQSL 398
           LG++ F    +   +FFF  N +     + SL
Sbjct: 785 LGKSVFIPLWLSGLLFFFF-NVYELKIILDSL 815
>dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei]
          Length = 430

 Score =  427 bits (1087), Expect = e-118
 Identities = 130/401 (32%), Positives = 205/401 (50%), Gaps = 27/401 (6%)

Query: 3   QLKYLITFSILLRFGFFFFGLYQDEYMPVKYTDIDYLVFNDASKFVYQGLSPYLRETYRY 62
            L+ L+    LLR     +  + D++  VKYTDIDY++  D ++ ++ G SP+ R T+RY
Sbjct: 11  SLQKLLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRY 70

Query: 63  TPILAILLIPDNFGKYWYHFGKLLFMVSDVITGLIILKLLSKQQQLSEKKKMILSSIWLL 122
           TP+LA L++P  +       GKL+F  SD+        +L K        K ++S   L 
Sbjct: 71  TPLLAALVMPSIWIAN--PMGKLIFASSDLGAAWYCYGVL-KSFAKERSAKWMVSLFILF 127

Query: 123 NPMVITISTRGSAESVLTVMIMLSLYYLLDKDNVILSAIWLGLSIHFKIYPIIYLPSILY 182
           NP+V+++STRG+++ ++T M ++ L     +     +A+ LG ++HFKIYPIIY      
Sbjct: 128 NPIVLSVSTRGNSDMLVTFMSLMVLSKFARRKCYQAAAV-LGFAVHFKIYPIIYALP--L 184

Query: 183 YLSSQETPFLASVPGINLVNAKNLKYIIITLT---TLAVVNYLMFLKYGWEFIDNSYLYH 239
            L   E    AS      V    +   I  L    + AV   L ++KYG ++++ +++YH
Sbjct: 185 TLGVWEQSVAASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEAFIYH 244

Query: 240 VTRLDHRHNFSVYNMVLYYKSALLEDSNGFDIEK--IAFVPQ-LLLSAVIIPLIFAKEDL 296
           V R DHRHNFS Y +++Y   A      G D     +AFVPQ ++LS V   L   + + 
Sbjct: 245 VYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKL---RRNT 301

Query: 297 ISSLFIQTFVFVAFNKVITSQYFIWFLIFLPHFLSKTKLLTTDK------------ITGI 344
             +  +QT +FVAFNKV T QYF+WF+ FL     + K +  D+            +  +
Sbjct: 302 AHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKAL 361

Query: 345 SCLLLWIISQATWLYFAYKLEFLGENTFDNGLMYSSVFFFL 385
             +L+W  +   W+  A  LEF G + F    + S +FF  
Sbjct: 362 GVVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLA 402
>ref|NP_012547.1| (NC_001142) Protein required for cell viability; Yjr013wp
           [Saccharomyces cerevisiae]
 sp|P47088|YJY3_YEAST HYPOTHETICAL 35.6 KD PROTEIN IN SPC1-ILV3 INTERGENIC REGION
 pir||S55201 hypothetical protein YJR013w - yeast (Saccharomyces cerevisiae)
 emb|CAA60935.1| (X87611) ORF YJR83.11 [Saccharomyces cerevisiae]
 emb|CAA89537.1| (Z49513) ORF YJR013w [Saccharomyces cerevisiae]
          Length = 305

 Score =  339 bits (863), Expect = 5e-92
 Identities = 137/306 (44%), Positives = 186/306 (60%), Gaps = 21/306 (6%)

Query: 99  LKLLSKQQQLSEKKKMILSSIWLLNPMVITISTRGSAESVLTVMIMLSLYYLLDKDNVIL 158
           +KLL+  Q +S K+ +IL SIWLLNPMVITISTRG+AESVL  +IM +L++L       L
Sbjct: 1   MKLLN--QAISRKRALILESIWLLNPMVITISTRGNAESVLCCLIMFTLFFLQKSR-YTL 57

Query: 159 SAIWLGLSIHFK---IYPIIYLPSILYYLSSQETPFLASVPGINLVNAKNLKYIIITLTT 215
           + I  GLSIHFK   I   I +   +YY    + P       +N+         + TLTT
Sbjct: 58  AGILYGLSIHFKIYPIIYCIPIAIFIYYNKRNQGPRTQLTSLLNIG--------LSTLTT 109

Query: 216 LAVVNYLMFLKYGWEFIDNSYLYHVTRLDHRHNFSVYNMVLYYKSALLEDSNGFDIEKIA 275
           L    + M+  YG+EF+D +YLYH+ R DHRHNFSV+NM+LY  SA  E+    ++ + A
Sbjct: 110 LLGCGWAMYKIYGYEFLDQAYLYHLYRTDHRHNFSVWNMLLYLDSANKENGES-NLSRYA 168

Query: 276 FVPQLLLSAVIIPLIFAK---EDLISSLFIQTFVFVAFNKVITSQYFIWFLIFLPHFLSK 332
           FVPQLLL  V   L +     ++L+  LF+QTF FV +NKV TSQYF+W+LIFLP +LS+
Sbjct: 169 FVPQLLLVLVTGCLEWWNPTFDNLLRVLFVQTFAFVTYNKVCTSQYFVWYLIFLPFYLSR 228

Query: 333 TKLLTTDKITGISCLLLWIISQATWLYFAYKLEFLGENTFDNGLMYSSVFFFLSNCWCTM 392
           T +       G+    LW+ +Q  WL   Y LEF G+N F  GL  +SV FF++N W   
Sbjct: 229 THIGWK---KGLLMATLWVGTQGIWLSQGYYLEFEGKNVFYPGLFIASVLFFVTNVWLLG 285

Query: 393 KFIQSL 398
           +FI  +
Sbjct: 286 QFITDI 291
>ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster]
 gb|AAF46748.1| (AE003454) CG9865 gene product [Drosophila melanogaster]
 gb|AAK77251.1| (AY047519) GH02741p [Drosophila melanogaster]
          Length = 466

 Score =  298 bits (757), Expect = 1e-79
 Identities = 97/288 (33%), Positives = 151/288 (51%), Gaps = 15/288 (5%)

Query: 119 IWLLNPMVITISTRGSAESVLTVMIMLSLYYLLDKDNVILSAIWL--------GLSIHFK 170
            WL NP+   ISTRGS +   +  ++L++Y LL  ++ +  + WL        GL IH +
Sbjct: 167 FWLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLR 226

Query: 171 IYPIIYLPSILYYLSSQ-ETPFLASVPGINLVNAKNLKYIIITLTTLAVVNYLMFLKYGW 229
           +YP+++  +    LS++     L  +  I   N + L  I  TL +L    +  +  YGW
Sbjct: 227 LYPLLFSLAYYLSLSTRLTQTPLDFLCQILRPNKQQLCLISGTLISLVAFTWTFYTMYGW 286

Query: 230 EFIDNSYLYHVTRLDHRHNFSVYNMVLYYKSALLEDSNGFDIEKIAFVPQLLLSAVIIPL 289
           E+I  +YLYH  R D RHNFS+  ++ Y  SA         ++ +   PQ LL  + + L
Sbjct: 287 EYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAPQFLL-ILYLSL 345

Query: 290 IFA--KEDLISSLFIQTFVFVAFNKVITSQYFIWFLIFLPHFLSKTKLLTTDKITGISCL 347
            F   ++ L   +F   FV V +N V+TSQYFIW+L  LP  L+  K+L+  +  G+  L
Sbjct: 346 SFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKMLSWRRCFGL--L 403

Query: 348 LLWIISQATWLYFAYKLEFLGENTFDNGLMYSSVFFFLSNCWCTMKFI 395
            LW+++QA WL  AY LEF   NTF    +  +VFF  +N +   + +
Sbjct: 404 FLWLLAQALWLLPAYLLEFHTWNTFYWIGLQGAVFF-ATNGYILEQLL 450
 Score =  140 bits (352), Expect = 3e-32
 Identities = 37/120 (30%), Positives = 59/120 (48%), Gaps = 4/120 (3%)

Query: 2   SQLKYLITFSILLRFGFFFFGLYQDEYMPVKYTDIDYLVFNDASKFVYQGLSPYLRETYR 61
           +  +  +  S LLR     +G   D    V YTDIDY V  D ++ V  G +P+ R TYR
Sbjct: 21  TSFRTHLLISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYR 80

Query: 62  YTPILAILLIPDNFGKYWYHFGKLLFMVSDVITGLIILKL--LSKQQQLSEKKKMILSSI 119
           Y+PI+A L   +        +GKLL+   D++   +I +L  +  + Q  +  + +LS  
Sbjct: 81  YSPIMAYLQTFNILL--HPAWGKLLYATFDLLIATLIYRLVHMEIKSQYQKTVQHLLSKF 138
>emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Arabidopsis thaliana]
          Length = 417

 Score =  270 bits (686), Expect = 3e-71
 Identities = 95/289 (32%), Positives = 142/289 (48%), Gaps = 42/289 (14%)

Query: 138 VLTVMIMLSLYYLLDKDNVILSAIWLGLSIHFKIYPIIYLPSILYYLSSQ-----ETPF- 191
           VL  + M  +Y+     N++ +A W GL +HF++YPIIY   I+  L +Q     + P  
Sbjct: 117 VLESISMFQIYW--SAGNLLQAAFWYGLVVHFRVYPIIYALPIILVLDTQVFRSGQKPAL 174

Query: 192 ------LASVPGINLVNAKNLKYIIITLTT----------------LAVVNYLMFLKYGW 229
                  A  P  N+     L  ++ TL +                    N + F  YG 
Sbjct: 175 LYWNTGQAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALISGGVFLACNAVSFYFYGQ 234

Query: 230 EFIDNSYLYHVTRLDHRHNFSVYNMVLYYKSALLEDSNGFDIEK-IAFVPQLLLSAVIIP 288
           EF+  + LYH+TR D RHNFS+Y   +Y       +     +EK I+F+PQ     V   
Sbjct: 235 EFLHEALLYHLTRTDPRHNFSIYFYHIYLH----YERQFSAVEKLISFLPQF---TVQFA 287

Query: 289 LIFA-KEDLISSLFIQTFVFVAFNKVITSQYFIWFLIFLPHFLSKTKLLTTDKITGISCL 347
           L+F   +DL+  +F+QT  FV FNKVIT+QYF+WF   LP  L  + +       G+ C+
Sbjct: 288 LVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKLK--WEGLLCI 345

Query: 348 LLWIISQATWLYFAYKLEFLGENTFDNGLMYSSVFFFLSNCWCTMKFIQ 396
           ++WI +Q  WL + Y LEF G N F   L  +S+ F  +N +  ++ IQ
Sbjct: 346 IMWIGAQTHWLLWGYMLEFKGVNVF-LPLWIASLLFLAANTFVLVRIIQ 393
 Score =  118 bits (295), Expect = 1e-25
 Identities = 41/88 (46%), Positives = 61/88 (68%), Gaps = 2/88 (2%)

Query: 3   QLKYLITFSILLRFGFFFFGLYQDEYMPVKYTDIDYLVFNDASKFVYQGLSPYLRETYRY 62
           + + L+ FS+LLR     +G +QD +M V+YTD+DY+VF+DA+  +  G SPY R TYRY
Sbjct: 17  KFRSLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYRY 76

Query: 63  TPILAILLIPDNFGKYWYHFGKLLFMVS 90
           +P+LA+LL P++F  +   +GK LF  S
Sbjct: 77  SPLLALLLTPNSF--FHRSWGKFLFSAS 102
>dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharomyces pombe]
          Length = 149

 Score =  165 bits (416), Expect = 1e-39
 Identities = 56/147 (38%), Positives = 94/147 (63%), Gaps = 3/147 (2%)

Query: 98  ILKLLSKQQQLSEKKKMILSSIWLLNPMVITISTRGSAESVLTVMIMLSLYYLLDKDNVI 157
           ++KLLS+  ++S K+ +I SS W+LNP V  ISTRG+ E++L ++ + +L YL++K +V 
Sbjct: 6   MIKLLSR--RISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSI-ALLYLIEKKSVW 62

Query: 158 LSAIWLGLSIHFKIYPIIYLPSILYYLSSQETPFLASVPGINLVNAKNLKYIIITLTTLA 217
           L+++ LG S+HFKIYP +Y  + L Y S  +         ++L++   LK ++ +L    
Sbjct: 63  LASLILGFSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSINQLKIVVGSLFMFT 122

Query: 218 VVNYLMFLKYGWEFIDNSYLYHVTRLD 244
           + N LM+  YG  F++++YLYH  R D
Sbjct: 123 ICNLLMYYLYGSPFLEHTYLYHFGRTD 149
>ref|NP_346994.1| (NC_003030) Uncharacterized membrane protein [Clostridium
           acetobutylicum]
 gb|AAK78334.1|AE007550_1 (AE007550) Uncharacterized membrane protein [Clostridium
           acetobutylicum]
          Length = 544

 Score =  104 bits (258), Expect = 4e-21
 Identities = 28/107 (26%), Positives = 62/107 (57%), Gaps = 4/107 (3%)

Query: 84  KLLFMVSDVITGLIILKLLSKQQQLSEKKKMILSSIWLLNPMVITISTR-GSAESVLTVM 142
           KL  +++D+ +   + +L    ++ S +    L+  +L NP ++  ST  G A+S  T++
Sbjct: 226 KLPSIIADIFSAYFVYRL--SIKRFSYETSFALTLFYLFNPAILINSTLWGQADSFFTLL 283

Query: 143 IMLSLYYLLDKDNVILSAIWLGLSIHFKIYPIIYLPSILYYLSSQET 189
           ++L++Y+ + +D ++ S+I+  LS+  K   II+LP +L++    + 
Sbjct: 284 VILAVYF-ISEDKIVPSSIFFALSVLMKPQGIIFLPLVLFWYIKNKN 329
>ref|NP_536724.1| (NM_080476) CDC91 cell division cycle 91-like 1 [Homo sapiens]
 emb|CAC14080.1| (AL118520) bA346K17.2 (A novel protein similar to the cell division
           control protein 91 (CDC91, YLR459W or L9122.2) from
           Yeast) [Homo sapiens]
          Length = 435

 Score = 36.4 bits (84), Expect = 0.81
 Identities = 71/390 (18%), Positives = 141/390 (35%), Gaps = 55/390 (14%)

Query: 8   ITFSILLRFGFF--FFGLYQDEYMPVKYTDIDYLVFNDASKFVYQGLSPYLRETYRYTPI 65
           +  ++ +R   F      +  E + V      +    +    +  G+SPY    +  TP+
Sbjct: 9   LVVAVTVRAALFRSSLAEFISERVEVVSPLSSWKRVVEGLSLLDLGVSPYSGAVFHETPL 68

Query: 66  LAILLIPDNFGKYWYHFGKLLFMVSDVITGLIIL--------------KLLSKQQQLSEK 111
           +  L        +   + +L+FM++D +T + +               KLL +  Q +  
Sbjct: 69  IIYLF------HFLIDYAELVFMITDALTAIALYFAIQDFNKVVFKKQKLLLELDQYAPD 122

Query: 112 KK-------------MILSSIWLLNPMVI----TISTRGSAESVLTVMIMLSLYYLLDKD 154
                          + ++  +LLNP  I      ST     +++   I+ ++     K 
Sbjct: 123 VAELIRTPMEMRYIPLKVALFYLLNPYTILSCVAKSTCAINNTLIAFFILTTI-----KG 177

Query: 155 NVILSAIWLGLSIHFKIYPIIYLPSILYYLSSQETPFLASVPG-INLVNAKNLKYIIITL 213
           +  LSAI+L L+ +  +YP+      L YL  ++   +        + + +     + +L
Sbjct: 178 SAFLSAIFLALATYQSLYPLTLFVPGLLYLLQRQYIPVKMKSKAFWIFSWEYAMMYVGSL 237

Query: 214 TTLAVVNYLMFLKYGWEFIDNSYLYHVTRLDHRHNFSVYNMVLYYKSALLEDSNGFDIEK 273
               ++    FL   W+FI   Y + ++  D   N  ++           E    F +  
Sbjct: 238 V--VIICLSFFLLSSWDFIPAVYGFILSVPDLTPNIGLFWYF------FAEMFEHFSL-F 288

Query: 274 IAFVPQLLLSAVIIPL-IFAKEDLISSLFIQTFVFVAFNKVITSQYFIWFLIFLPHFLSK 332
              V Q+ +    IPL I  KE  I  +FIQ  V   F    T      ++ F P +   
Sbjct: 289 FVCVFQINVFFYTIPLAIKLKEHPIFFMFIQIAVIAIFKSYPTVGDVALYMAFFPVWNHL 348

Query: 333 TKLLTTDKITGISCLLLWIISQATWLYFAY 362
            + L    +     ++  ++    W  + Y
Sbjct: 349 YRFLRNIFVLTCIIIVCSLLFPVLWHLWIY 378
>ref|NP_563912.1| (NM_101143) expressed protein [Arabidopsis thaliana]
 gb|AAK44067.1|AF370252_1 (AF370252) unknown protein [Arabidopsis thaliana]
 gb|AAL34242.1| (AY063068) unknown protein [Arabidopsis thaliana]
          Length = 474

 Score = 35.6 bits (82), Expect = 1.4
 Identities = 32/158 (20%), Positives = 68/158 (42%), Gaps = 27/158 (17%)

Query: 53  SPYLRETYRYTPILAILLIP----DNFGKYWYHFGKLLFMVSDVITGLIILKLLSKQQQL 108
           SPY    Y  +P+L  +L P       G+  +H   L+F+++D+++  ++L+ + ++ Q 
Sbjct: 70  SPYAGSMYHGSPLLLSILGPLTVQRIEGQSSHHLCSLVFVIADLLSA-LLLRAIGQKLQK 128

Query: 109 SEKKKM-------------------ILSSIWLLNPMVITISTRGSAESVLTVMIMLSLYY 149
           S +  +                   I + ++L NP    +S  G + S +  + ++   Y
Sbjct: 129 SYRMNLRPLGVLRSSQDRGILPCGDIAALVYLWNPFT-ILSCVGLSTSPIENLAVIVSLY 187

Query: 150 LLDKDNVILSAIWLGLSIHFKIY--PIIYLPSILYYLS 185
                 V L+A  L ++ H  +Y  P+++    L    
Sbjct: 188 GAVTRRVPLAAFGLIMATHLSLYSAPLVFPLIFLLGYG 225
>ref|NP_488389.1| (NC_003272) unknown protein [Nostoc sp. PCC 7120]
 dbj|BAB76048.1| (AP003596) ORF_ID:all4349~unknown protein [Nostoc sp. PCC 7120]
          Length = 399

 Score = 35.2 bits (81), Expect = 1.8
 Identities = 35/169 (20%), Positives = 65/169 (37%), Gaps = 9/169 (5%)

Query: 27  EYMPVKYTDIDYLVFNDA--SKFVYQGLSPYLRETYR-YT-PILAILLIPDNFGKYWYHF 82
             + V    +DY  F        V  G    L+ ++  YT P L  +LI           
Sbjct: 43  RLICVPNKSVDYKYFLAPWYDFIVSNGGFSALKYSFADYTPPYLYWILIAGTVLSGLPKI 102

Query: 83  G--KLLFMVSDVITGLIILKLLSKQQQLSEKKKMILSSIWLLNPMVITISTR-GSAESVL 139
              KL  M  D +      K++  +     K         +L+P+VI  S   G  +S+ 
Sbjct: 103 LAIKLFAMSVDFVCAFFTYKIVKLKYPEGRKAIFAF-ITVILSPVVIYNSALWGQCDSIY 161

Query: 140 TVMIMLSLYYLLDKDNVILSAIWLGLSIHFKIYPIIYLPSILYYLSSQE 188
           T  ++  +Y+L  +  +  + +  G+++ FK+  +   P +L +L    
Sbjct: 162 TTGLIACIYFLSIQKQLS-ALVSFGIALSFKLQAMFLSPLLLIFLIKNR 209
>ref|XP_079016.1| (XM_079016) CG13089 [Drosophila melanogaster]
 gb|AAF52689.1| (AE003621) CG13089 gene product [Drosophila melanogaster]
 gb|AAL13897.1| (AY058668) LD37974p [Drosophila melanogaster]
          Length = 426

 Score = 33.3 bits (76), Expect = 7.6
 Identities = 34/230 (14%), Positives = 80/230 (34%), Gaps = 40/230 (17%)

Query: 50  QGLSPYLRETYRYTPILAILLIPDNFGKYWYHFGKLLFMVSDVITGLIILKLLSKQQQLS 109
            G+ PYL +    +P+  IL       + +  F  + +++ D+ T  ++  +  +  +  
Sbjct: 53  SGIDPYLGDLVHESPL--ILSALSGLFQKYPQFLPIFYIILDICTAALLYAMSLRFVKQK 110

Query: 110 EKKK-------------------------MILSSIWLLNPMVITISTRGSAESVLTVMIM 144
           + ++                          ++   +L +P+   +S  G   +V++ + +
Sbjct: 111 QDQQDKERKEYAKDTEELQFGPLDKLDIPELVIVAYLFSPLT-VMSCIGMTSTVISNLFL 169

Query: 145 LSLYYLLDKDNVILSAIWLGLSIHFKIYPIIYLPSILYYLSSQETPFLASVPGINLVNAK 204
              +Y L K  +I   + L        YPI+ +  +L   S            +    A 
Sbjct: 170 AFFFYCLVKGMLIPCLLVLAFETVRSFYPIVLIAPLLLVFSRNS---------VRRGVAI 220

Query: 205 NLKYIIITLTTLAVVNYLMFLKYGWEFIDNSYLYHVTRLDHRHNFSVYNM 254
              +I+  L          F+   W F+D +  +     D + N  ++  
Sbjct: 221 AALFIVSCLIVAVA---NYFVLNSWNFLDGTLGFIFYFRDLQPNIGLFWY 267
CPU time:   139.93 user secs.	    2.28 sys. secs	  142.21 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.320    0.183    0.585 

Gapped
Lambda     K      H
   0.270   0.0643    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 327426957
Number of Sequences: 887402
Number of extensions: 19126312
Number of successful extensions: 69188
Number of sequences better than 10.0: 291
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 267
Number of HSP's that attempted gapping in prelim test: 68763
Number of HSP's gapped (non-prelim): 512
length of query: 398
length of database: 277,845,442
effective HSP length: 52
effective length of query: 346
effective length of database: 231,700,538
effective search space: 80168386148
effective search space used: 80168386148
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 75 (33.2 bits)