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GPI Anchor Biosynthesis Report: CAC34506.1 (PIG-M family, Arabidopsis thaliana)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (417 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 20 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Ara... 588 e-167
ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase ... 382 e-105
dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:19148... 382 e-105
ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosylt... 381 e-104
gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mu... 380 e-104
ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans] ... 321 1e-86
emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [S... 297 3e-79
dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei] 292 5e-78
ref|NP_012547.1| (NC_001142) Protein required for cell viability... 275 8e-73
ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster] >g... 269 8e-71
dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharom... 117 2e-25
Sequences with E-value WORSE than threshold

pir||T36589 probable transmembrane protein - Streptomyces coelic... 35 2.5
Alignments
>emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Arabidopsis thaliana]
          Length = 417

 Score =  588 bits (1502), Expect = e-167
 Identities = 417/417 (100%), Positives = 417/417 (100%)

Query: 1   MSRESENTDDEPSKWMKFRSLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAAS 60
           MSRESENTDDEPSKWMKFRSLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAAS
Sbjct: 1   MSRESENTDDEPSKWMKFRSLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAAS 60

Query: 61  LMASGESPYKRTTYRYSPLLALLLTPNSFFHRSWGKFLFSASVHQPHNVSMICFSVVLES 120
           LMASGESPYKRTTYRYSPLLALLLTPNSFFHRSWGKFLFSASVHQPHNVSMICFSVVLES
Sbjct: 61  LMASGESPYKRTTYRYSPLLALLLTPNSFFHRSWGKFLFSASVHQPHNVSMICFSVVLES 120

Query: 121 ISMFQIYWSAGNLLQAAFWYGLVVHFRVYPIIYALPIILVLDTQVFRSGQKPALLYWNTG 180
           ISMFQIYWSAGNLLQAAFWYGLVVHFRVYPIIYALPIILVLDTQVFRSGQKPALLYWNTG
Sbjct: 121 ISMFQIYWSAGNLLQAAFWYGLVVHFRVYPIIYALPIILVLDTQVFRSGQKPALLYWNTG 180

Query: 181 QAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALISGGVFLACNAVSFYFYGQEFLHEA 240
           QAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALISGGVFLACNAVSFYFYGQEFLHEA
Sbjct: 181 QAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALISGGVFLACNAVSFYFYGQEFLHEA 240

Query: 241 LLYHLTRTDPRHNFSIYFYHIYLHYERQFSAVEKLISFLPQFTVQFALVFCFSQDLVFCI 300
           LLYHLTRTDPRHNFSIYFYHIYLHYERQFSAVEKLISFLPQFTVQFALVFCFSQDLVFCI
Sbjct: 241 LLYHLTRTDPRHNFSIYFYHIYLHYERQFSAVEKLISFLPQFTVQFALVFCFSQDLVFCI 300

Query: 301 FLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGY 360
           FLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGY
Sbjct: 301 FLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGY 360

Query: 361 MLEFKGVNVFLPLWIASLLFLAANTFVLVRIIQRHRFSPLFRRYESSSSSNNVTKED 417
           MLEFKGVNVFLPLWIASLLFLAANTFVLVRIIQRHRFSPLFRRYESSSSSNNVTKED
Sbjct: 361 MLEFKGVNVFLPLWIASLLFLAANTFVLVRIIQRHRFSPLFRRYESSSSSNNVTKED 417
>ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase [Rattus norvegicus]
 dbj|BAB18566.1| (AB028126) mannosyltransferase [Rattus norvegicus]
          Length = 423

 Score =  382 bits (972), Expect = e-105
 Identities = 116/316 (36%), Positives = 162/316 (50%), Gaps = 28/316 (8%)

Query: 89  FFHRSWGKFLFSASVHQPHNVSMICFSVVLESISMFQIYWSAGNLLQ-AAFWYGLVVHFR 147
           +            +V    N   I       S+ +  +Y     L+  AA +YG  VH +
Sbjct: 131 WLLNPLP-----MAVSSRGNADSIVA-----SLVLTTLYLIEKRLIACAAVFYGFAVHMK 180

Query: 148 VYPIIYALPIILVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFS 207
           +YP+ Y LPI L L  +                +       + R +F   L   LK L S
Sbjct: 181 MYPVTYILPIALHLRPE----------------RDSDEGLRLARYSFQARLYDFLKRLCS 224

Query: 208 RERIMFALISGGVFLACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYER 267
              ++F  I+G  FLA +   +Y YG EFL    LYHLTR D RHNFS YFY +YL  E 
Sbjct: 225 WAVLLFVAIAGLTFLALSFGFYYKYGWEFLEHTYLYHLTRRDIRHNFSPYFYMLYLTAES 284

Query: 268 QFSAVEKLISFLPQFTVQFALVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLP 327
           ++S    + +FLPQF +  A  F + +DLVFC FL T  FVTFNKV T+QYF+W+ CLLP
Sbjct: 285 KWSFTLGIAAFLPQFILLSAASFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLP 344

Query: 328 LILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFV 387
           L++P   M  K   ++ +++W   Q  WL   Y+LEF+G N FL +W+A L FL  N  +
Sbjct: 345 LVMPLVRMPWK-RAVVLLMLWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSI 403

Query: 388 LVRIIQRHRFSPLFRR 403
           L++II  ++   L  R
Sbjct: 404 LIQIISHYKEDRLTER 419
 Score =  117 bits (291), Expect = 5e-25
 Identities = 42/76 (55%), Positives = 53/76 (69%)

Query: 24 FSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYRYSPLLALL 83
           + L RV L+ YG +QD  + VRYTD+DY VF+DAA  +  G SPY R TYRY+PLL+ L
Sbjct: 24 VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 84 LTPNSFFHRSWGKFLF 99
          LTPN +    +GKFLF
Sbjct: 84 LTPNVYLSELFGKFLF 99
>dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:1914806,
           evidence:ISS~phosphatidylinositol glycan, class
           M~putative [Mus musculus]
          Length = 423

 Score =  382 bits (971), Expect = e-105
 Identities = 112/316 (35%), Positives = 160/316 (50%), Gaps = 28/316 (8%)

Query: 89  FFHRSWGKFLFSASVHQPHNVSMICFSVVLESISMFQIYWSAGNLLQ-AAFWYGLVVHFR 147
           +            +V    N   I       S+ +  +Y+    L+  AA +YG  VH +
Sbjct: 131 WLLNPLP-----MAVSSRGNADSIVA-----SLVLSTLYFIEKRLIACAAVFYGFAVHMK 180

Query: 148 VYPIIYALPIILVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFS 207
           +YP+ Y LPI L L  +                +         R +F   L   L+ L S
Sbjct: 181 MYPVTYILPIALHLRPE----------------RDDDERLRQARFSFQARLYDFLRRLCS 224

Query: 208 RERIMFALISGGVFLACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYER 267
              ++F  ++G  F+A +   +Y YG EFL     YHLTR D RHNFS YFY +YL  E 
Sbjct: 225 WAVLLFVAVAGLTFVALSFGFYYKYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAES 284

Query: 268 QFSAVEKLISFLPQFTVQFALVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLP 327
           ++S    + +FLPQF +  A  F + +DLVFC FL T  FVTFNKV T+QYF+W+ CLLP
Sbjct: 285 KWSFTLGIAAFLPQFILLSAASFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLP 344

Query: 328 LILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFV 387
           L++P   M  K   ++ ++ W   Q  WL   Y+LEF+G N FL +W+A L FL  N  +
Sbjct: 345 LVMPLVRMPWK-RAVVLLLFWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSI 403

Query: 388 LVRIIQRHRFSPLFRR 403
           L++II  ++   L  R
Sbjct: 404 LIQIISHYKEDRLTER 419
 Score =  116 bits (290), Expect = 5e-25
 Identities = 42/76 (55%), Positives = 53/76 (69%)

Query: 24 FSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYRYSPLLALL 83
           + L RV L+ YG +QD  + VRYTD+DY VF+DAA  +  G SPY R TYRY+PLL+ L
Sbjct: 24 VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 84 LTPNSFFHRSWGKFLF 99
          LTPN +    +GKFLF
Sbjct: 84 LTPNVYLSELFGKFLF 99
>ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosyltransferase [Homo
           sapiens]
 dbj|BAB18567.1| (AB028127) mannosyltransferase [Homo sapiens]
 gb|AAH19865.1|AAH19865 (BC019865) Unknown (protein for MGC:29896) [Homo sapiens]
          Length = 423

 Score =  381 bits (970), Expect = e-104
 Identities = 114/316 (36%), Positives = 161/316 (50%), Gaps = 28/316 (8%)

Query: 89  FFHRSWGKFLFSASVHQPHNVSMICFSVVLESISMFQIYWSAGNLLQ-AAFWYGLVVHFR 147
           +            +V    N   I       S+ +  +Y     L+  AA +YG  VH +
Sbjct: 131 WLLNPLP-----MAVSSRGNADSIVA-----SLVLMVLYLIKKRLVACAAVFYGFAVHMK 180

Query: 148 VYPIIYALPIILVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFS 207
           +YP+ Y LPI L L                   +    +    R TF   L   LK L +
Sbjct: 181 IYPVTYILPITLHLLPD----------------RDNDKSLRQFRYTFQACLYELLKRLCN 224

Query: 208 RERIMFALISGGVFLACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYER 267
           R  ++F  ++G  F A +   +Y YG EFL     YHLTR D RHNFS YFY +YL  E 
Sbjct: 225 RAVLLFVAVAGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAES 284

Query: 268 QFSAVEKLISFLPQFTVQFALVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLP 327
           ++S    + +FLPQ  +  A+ F + +DLVFC FL T  FVTFNKV T+QYF+W+ CLLP
Sbjct: 285 KWSFSLGIAAFLPQLILLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLP 344

Query: 328 LILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFV 387
           L++P   M  K   ++ +++W   Q  WL   Y+LEF+G N FL +W+A L FL  N  +
Sbjct: 345 LVMPLVRMPWK-RAVVLLMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSI 403

Query: 388 LVRIIQRHRFSPLFRR 403
           L++II  ++  PL  R
Sbjct: 404 LIQIISHYKEEPLTER 419
 Score =  118 bits (294), Expect = 2e-25
 Identities = 42/76 (55%), Positives = 52/76 (68%)

Query: 24 FSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYRYSPLLALL 83
           + L RV L+ YG +QD  + VRYTD+DY VF+DAA  +  G SPY R TYRY+PLL  L
Sbjct: 24 VAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLGWL 83

Query: 84 LTPNSFFHRSWGKFLF 99
          LTPN +    +GKFLF
Sbjct: 84 LTPNIYLSELFGKFLF 99
>gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mus musculus]
          Length = 423

 Score =  380 bits (967), Expect = e-104
 Identities = 112/316 (35%), Positives = 160/316 (50%), Gaps = 28/316 (8%)

Query: 89  FFHRSWGKFLFSASVHQPHNVSMICFSVVLESISMFQIYWSAGNLLQ-AAFWYGLVVHFR 147
           +            +V    N   I       S+ +  +Y+    L+  AA +YG  VH +
Sbjct: 131 WLLNPLP-----MAVSSRGNADSIVA-----SLVLSTLYFIEKRLIACAAVFYGFAVHMK 180

Query: 148 VYPIIYALPIILVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFS 207
           +YP+ Y LPI L L  +                +         R +F   L   L+ L S
Sbjct: 181 MYPVTYILPIALHLRPE----------------RDDDERLRHARFSFQARLYDFLRRLCS 224

Query: 208 RERIMFALISGGVFLACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYER 267
              ++F  ++G  F+A +   +Y YG EFL     YHLTR D RHNFS YFY +YL  E 
Sbjct: 225 WAVLLFVAVAGLTFVALSFGFYYKYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAES 284

Query: 268 QFSAVEKLISFLPQFTVQFALVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLP 327
           ++S    + +FLPQF +  A  F + +DLVFC FL T  FVTFNKV T+QYF+W+ CLLP
Sbjct: 285 KWSFTLGIAAFLPQFILLSAASFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLP 344

Query: 328 LILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFV 387
           L++P   M  K   ++ ++ W   Q  WL   Y+LEF+G N FL +W+A L FL  N  +
Sbjct: 345 LVMPLVRMPWK-RAVVLLLFWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSI 403

Query: 388 LVRIIQRHRFSPLFRR 403
           L++II  ++   L  R
Sbjct: 404 LIQIISHYKEDRLTER 419
 Score =  116 bits (290), Expect = 5e-25
 Identities = 42/76 (55%), Positives = 53/76 (69%)

Query: 24 FSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYRYSPLLALL 83
           + L RV L+ YG +QD  + VRYTD+DY VF+DAA  +  G SPY R TYRY+PLL+ L
Sbjct: 24 VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 84 LTPNSFFHRSWGKFLF 99
          LTPN +    +GKFLF
Sbjct: 84 LTPNVYLSELFGKFLF 99
>ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans]
 pir||T18769 hypothetical protein B0491.1 - Caenorhabditis elegans
 emb|CAA90090.1| (Z49907) B0491.1 [Caenorhabditis elegans]
          Length = 417

 Score =  321 bits (816), Expect = 1e-86
 Identities = 103/309 (33%), Positives = 149/309 (47%), Gaps = 39/309 (12%)

Query: 89  FFHRSWGKFLFSASVHQPHNVSMICFSVVLESISMFQI-YWSAGNLLQAAFWYGLVVHFR 147
           +          +A +    N   I  +VVL +I + Q  YW +  L+  A    L +  +
Sbjct: 137 WLANPL-----TAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGA----LAIQLK 187

Query: 148 VYPIIYALPIILVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFS 207
           +YP+IY   + L L T     G++  ++     + K+  SN +   ++   LT+      
Sbjct: 188 IYPLIYLPSVFLSLST----IGEQSCVV----NKFKSLVSNWKGFAYMLVTLTS------ 233

Query: 208 RERIMFALISGGVFLACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYER 267
                        F A     F  YGQ FL E L+YH+ R D  HNFS YFY +YL+   
Sbjct: 234 -------------FAAVVLFFFQIYGQLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEAN 280

Query: 268 -QFSAVEKLISFLPQFTVQFALVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLL 326
              S +  L +F+PQ  +     F    DL FC F+ T AFVT+NKV T+QYFVW+  LL
Sbjct: 281 PTMSQIIGLGAFIPQIVLIVFFAFKHYDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLL 340

Query: 327 PLILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTF 386
           PL L    M  +   L  +  W   Q  WLL  Y+ EF+G N F  +++AS LFL AN+F
Sbjct: 341 PL-LAHKIMMSRQLALSLMAAWFATQGIWLLAAYLFEFQGWNTFFLMFLASCLFLIANSF 399

Query: 387 VLVRIIQRH 395
           +L +II  +
Sbjct: 400 ILKQIINHY 408
 Score =  118 bits (295), Expect = 1e-25
 Identities = 34/82 (41%), Positives = 50/82 (60%), Gaps = 1/82 (1%)

Query: 20  SLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYRYSPL 79
            +L+ + + R+ L+ Y    D   +V +TD+DY VFSDAA  +++G SP+ R TYRY+P 
Sbjct: 17  KILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPA 76

Query: 80  LALLLTPNSFFHRSWGKFLFSA 101
           LA +L P   F   +GK LF  
Sbjct: 77  LAWILLPVVHF-PDFGKILFCI 97
>emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [Schizosaccharomyces
           pombe]
          Length = 815

 Score =  297 bits (753), Expect = 3e-79
 Identities = 101/313 (32%), Positives = 153/313 (48%), Gaps = 44/313 (14%)

Query: 83  LLTPNSFFHRSWGKFLFSASVHQPHNVSMICFSVVLESISMFQIYWSAGNLL-QAAFWYG 141
           L+  + +         F A +    N   I     L  +S+  +Y      +  A+   G
Sbjct: 541 LIYSSFWILNP-----FVAIISTRGNCEAI-----LGILSIALLYLIEKKSVWLASLILG 590

Query: 142 LVVHFRVYPIIYALPIILVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTT 201
             VHF++YP +Y +  ++                     + K  ++ ME+          
Sbjct: 591 FSVHFKIYPFMYGIAFLVYFS------------------KPKKGSTFMEKF--------- 623

Query: 202 LKSLFSRERIMFALISGGVFLACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHI 261
             SL S  ++   + S  +F  CN + +Y YG  FL    LYH  RTD RHNFS+  +H+
Sbjct: 624 -LSLLSINQLKIVVGSLFMFTICNLLMYYLYGSPFLEHTYLYHFGRTDHRHNFSL--HHL 680

Query: 262 YLHYERQFSA-VEKLISFLPQFTVQFALVFCF-SQDLVFCIFLQTVAFVTFNKVITAQYF 319
            L+YE  F A    L +FLPQ ++   +   F  ++L   +F QT AFVTFNKV T+QYF
Sbjct: 681 NLYYESSFGAKASSLFAFLPQLSLCMLIPLVFGKKNLPGTLFAQTFAFVTFNKVCTSQYF 740

Query: 320 VWFYCLLPLILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLL 379
           +W+   LPL+LP S + L  +GL+C+ +WI  Q  WL+  Y LE  G +VF+PLW++ LL
Sbjct: 741 MWYLVFLPLVLPNSKL-LSKKGLICLSLWIIGQLLWLISAYNLEMLGKSVFIPLWLSGLL 799

Query: 380 FLAANTFVLVRII 392
           F   N + L  I+
Sbjct: 800 FFFFNVYELKIIL 812
 Score =  106 bits (263), Expect = 9e-22
 Identities = 41/82 (50%), Positives = 58/82 (70%)

Query: 21  LLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYRYSPLL 80
           LL+  +L+R+ L+ YG W D    +++TD+DY VFSDA+  ++ G SPY R TYRY+P+L
Sbjct: 438 LLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSPYMRDTYRYTPML 497

Query: 81  ALLLTPNSFFHRSWGKFLFSAS 102
           A+LL P  +   SWGK+LFS S
Sbjct: 498 AILLLPTQYGFPSWGKYLFSIS 519
>dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei]
          Length = 430

 Score =  292 bits (742), Expect = 5e-78
 Identities = 86/303 (28%), Positives = 131/303 (42%), Gaps = 50/303 (16%)

Query: 100 SASVHQPHNVSMICFSVVLESISMFQIYWSAGNLLQAAFWYGLVVHFRVYPIIYALPIIL 159
             SV    N  M+   V   S+ +   +       QAA   G  VHF++YPIIYALP+ L
Sbjct: 131 VLSVSTRGNSDML---VTFMSLMVLSKFARRKCY-QAAAVLGFAVHFKIYPIIYALPLTL 186

Query: 160 VLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALISGG 219
            +                   Q+   ++N  R+     ++ ++           AL++  
Sbjct: 187 GV-----------------WEQSVAASTNTWRRVVKTAVVVSIC----------ALMAAI 219

Query: 220 VFLACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYERQ-----FSAVEK 274
            F     + +  YGQ++L+EA +YH+ R D RHNFS Y+  +YL+  R+          +
Sbjct: 220 SFAVPTVLCYMKYGQQYLNEAFIYHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPR 279

Query: 275 LISFLPQFTVQFALVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLPLIL---- 330
           L++F+PQ  V   + +   ++      +QTV FV FNKV T QYFVWF   L  +     
Sbjct: 280 LVAFVPQAVVLSFVSYKLRRNTAHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPK 339

Query: 331 ---------PWSHMKLKW-EGLLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLF 380
                      +     W + L  ++MW      W+     LEF G + F  LWI S LF
Sbjct: 340 EVEDDESGGSGAFKFFSWVKALGVVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLF 399

Query: 381 LAA 383
             A
Sbjct: 400 FLA 402
 Score =  114 bits (285), Expect = 3e-24
 Identities = 36/87 (41%), Positives = 55/87 (62%)

Query: 16  MKFRSLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYR 75
           +  + LL+   LLR+ LI Y  + D    V+YTD+DY++  D A  M +G SP+ RTT+R
Sbjct: 10  VSLQKLLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFR 69

Query: 76  YSPLLALLLTPNSFFHRSWGKFLFSAS 102
           Y+PLLA L+ P+ +     GK +F++S
Sbjct: 70  YTPLLAALVMPSIWIANPMGKLIFASS 96
>ref|NP_012547.1| (NC_001142) Protein required for cell viability; Yjr013wp
           [Saccharomyces cerevisiae]
 sp|P47088|YJY3_YEAST HYPOTHETICAL 35.6 KD PROTEIN IN SPC1-ILV3 INTERGENIC REGION
 pir||S55201 hypothetical protein YJR013w - yeast (Saccharomyces cerevisiae)
 emb|CAA60935.1| (X87611) ORF YJR83.11 [Saccharomyces cerevisiae]
 emb|CAA89537.1| (Z49513) ORF YJR013w [Saccharomyces cerevisiae]
          Length = 305

 Score =  275 bits (698), Expect = 8e-73
 Identities = 102/320 (31%), Positives = 152/320 (46%), Gaps = 55/320 (17%)

Query: 83  LLTPNSFFHRSWGKFLFSASVHQPHNVSMICFSVVLESISMFQIYW-SAGNLLQAAFWYG 141
           L+  + +            ++    N        VL  + MF +++        A   YG
Sbjct: 14  LILESIWLLNPM-----VITISTRGNAES-----VLCCLIMFTLFFLQKSRYTLAGILYG 63

Query: 142 LVVHFRVYPIIYALPIILVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTT 201
           L +HF++YPIIY +PI +               +Y+N  + + P +              
Sbjct: 64  LSIHFKIYPIIYCIPIAI--------------FIYYNK-RNQGPRTQ------------- 95

Query: 202 LKSLFSRERIMFALISGGVFLACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHI 261
           L SL +       L +    L C    +  YG EFL +A LYHL RTD RHNFS++   +
Sbjct: 96  LTSLLNIG-----LSTLTTLLGCGWAMYKIYGYEFLDQAYLYHLYRTDHRHNFSVWNMLL 150

Query: 262 YLHYERQFSAVEKL--ISFLPQFTVQFALVFCFS------QDLVFCIFLQTVAFVTFNKV 313
           YL    + +    L   +F+PQ  +   +  C         +L+  +F+QT AFVT+NKV
Sbjct: 151 YLDSANKENGESNLSRYAFVPQLLLVL-VTGCLEWWNPTFDNLLRVLFVQTFAFVTYNKV 209

Query: 314 ITAQYFVWFYCLLPLILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGYMLEFKGVNVFLP- 372
            T+QYFVW+   LP  L  +H+  K +GLL   +W+G Q  WL  GY LEF+G NVF P 
Sbjct: 210 CTSQYFVWYLIFLPFYLSRTHIGWK-KGLLMATLWVGTQGIWLSQGYYLEFEGKNVFYPG 268

Query: 373 LWIASLLFLAANTFVLVRII 392
           L+IAS+LF   N ++L + I
Sbjct: 269 LFIASVLFFVTNVWLLGQFI 288
>ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster]
 gb|AAF46748.1| (AE003454) CG9865 gene product [Drosophila melanogaster]
 gb|AAK77251.1| (AY047519) GH02741p [Drosophila melanogaster]
          Length = 466

 Score =  269 bits (681), Expect = 8e-71
 Identities = 95/326 (29%), Positives = 148/326 (45%), Gaps = 53/326 (16%)

Query: 89  FFHRSWGKFLFSASVHQPHNVSMICFSVVLESISMFQIYWSAGNLLQ-------AAFWYG 141
           + +        +A +    +    CFS     ++++ +  S  N+ +       A   +G
Sbjct: 168 WLYNPL-----TAVISTRGSGD--CFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHG 220

Query: 142 LVVHFRVYPIIYALPIILVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTT 201
           LV+H R+YP++++L   L L T++                 +TP             L  
Sbjct: 221 LVIHLRLYPLLFSLAYYLSLSTRL----------------TQTP-------------LDF 251

Query: 202 LKSLFSRERIMFALISGGVF-LACNAVSFY-FYGQEFLHEALLYHLTRTDPRHNFSIYFY 259
           L  +    +    LISG +  L     +FY  YG E+++EA LYH  R DPRHNFS+ F 
Sbjct: 252 LCQILRPNKQQLCLISGTLISLVAFTWTFYTMYGWEYIYEAYLYHFVRKDPRHNFSLQFL 311

Query: 260 HIYLHYERQF---SAVEKLISFLPQFTVQFALVFC---FSQDLVFCIFLQTVAFVTFNKV 313
             YL         SA+ K +   PQF +   L      F Q L FCIF      VT+N V
Sbjct: 312 LQYLGSASSVAEPSAILKTLVLAPQFLLILYLSLSFGQFRQTLPFCIFAVAFVIVTYNSV 371

Query: 314 ITAQYFVWFYCLLPLILPWSHMKLKWEGLLCIIM-WIGAQTHWLLWGYMLEFKGVNVFLP 372
           +T+QYF+W+  +LPL L    M L W     ++  W+ AQ  WLL  Y+LEF   N F  
Sbjct: 372 VTSQYFIWYLAILPLCLNNFKM-LSWRRCFGLLFLWLLAQALWLLPAYLLEFHTWNTFYW 430

Query: 373 LWIASLLFLAANTFVLVRIIQRHRFS 398
           + +   +F A N ++L +++  + F+
Sbjct: 431 IGLQGAVFFATNGYILEQLLSHYGFT 456
 Score =  126 bits (314), Expect = 9e-28
 Identities = 39/86 (45%), Positives = 57/86 (65%)

Query: 15  WMKFRSLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTY 74
           +  FR+ L+ S LLR+ LI YG+  D+   V YTD+DY V +D A  + +G++P+ R TY
Sbjct: 20  YTSFRTHLLISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTY 79

Query: 75  RYSPLLALLLTPNSFFHRSWGKFLFS 100
           RYSP++A L T N   H +WGK L++
Sbjct: 80  RYSPIMAYLQTFNILLHPAWGKLLYA 105
>dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharomyces pombe]
          Length = 149

 Score =  117 bits (293), Expect = 2e-25
 Identities = 37/168 (22%), Positives = 62/168 (36%), Gaps = 39/168 (23%)

Query: 83  LLTPNSFFHRSWGKFLFSASVHQPHNVSMICFSVVLESISMFQIYWSAGNLL-QAAFWYG 141
           L+  + +         F A +    N   I     L  +S+  +Y      +  A+   G
Sbjct: 20  LIYSSFWILNP-----FVAIISTRGNCEAI-----LGILSIALLYLIEKKSVWLASLILG 69

Query: 142 LVVHFRVYPIIYALPIILVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTT 201
             VHF++YP +Y +  ++                     + K  ++ ME+          
Sbjct: 70  FSVHFKIYPFMYGIAFLVYFS------------------KPKKGSTFMEKF--------- 102

Query: 202 LKSLFSRERIMFALISGGVFLACNAVSFYFYGQEFLHEALLYHLTRTD 249
             SL S  ++   + S  +F  CN + +Y YG  FL    LYH  RTD
Sbjct: 103 -LSLLSINQLKIVVGSLFMFTICNLLMYYLYGSPFLEHTYLYHFGRTD 149
>pir||T36589 probable transmembrane protein - Streptomyces coelicolor
 emb|CAB42730.1| (AL049826) putative transmembrane protein [Streptomyces coelicolor
           A3(2)]
          Length = 502

 Score = 35.0 bits (80), Expect = 2.5
 Identities = 17/60 (28%), Positives = 22/60 (36%), Gaps = 4/60 (6%)

Query: 301 FLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKLKWEGLLCIIMWIGAQTHWLLWGY 360
           FL   AF+  NKV + QY +W   L  L  P     L W+              W+   Y
Sbjct: 350 FLIVAAFILTNKVYSPQYVLWLVPLAALARPKWRDFLIWQACEVAYF----LGIWMYLAY 405
CPU time:   112.63 user secs.	    2.31 sys. secs	  114.94 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.319    0.167    0.527 

Gapped
Lambda     K      H
   0.270   0.0586    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 278791807
Number of Sequences: 887402
Number of extensions: 14289880
Number of successful extensions: 53855
Number of sequences better than 10.0: 203
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 186
Number of HSP's that attempted gapping in prelim test: 53616
Number of HSP's gapped (non-prelim): 340
length of query: 417
length of database: 277,845,442
effective HSP length: 51
effective length of query: 366
effective length of database: 232,587,940
effective search space: 85127186040
effective search space used: 85127186040
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.0 bits)
S2: 75 (33.3 bits)