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GPI Anchor Biosynthesis Report: CAB89880.1 (PIG-M family, Schizosaccharomyces pombe)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (411 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 16 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [S... 527 e-148
ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase ... 514 e-144
dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:19148... 512 e-144
gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mu... 512 e-144
ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosylt... 511 e-144
ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans] ... 472 e-132
dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei] 452 e-126
emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Ara... 412 e-114
ref|NP_012547.1| (NC_001142) Protein required for cell viability... 339 5e-92
ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster] >g... 293 2e-78
dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharom... 170 5e-41
ref|NP_346994.1| (NC_003030) Uncharacterized membrane protein [C... 108 2e-22
Sequences with E-value WORSE than threshold

ref|NP_536724.1| (NM_080476) CDC91 cell division cycle 91-like 1... 37 0.63
ref|NP_126953.1| (NC_000868) hypothetical protein [Pyrococcus ab... 34 4.9
pir||T37929 probable major facilitator protein - fission yeast ... 33 7.6
Alignments
>emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [Schizosaccharomyces
           pombe]
          Length = 815

 Score =  527 bits (1344), Expect = e-148
 Identities = 411/411 (100%), Positives = 411/411 (100%)

Query: 1   MKLLSKYNSRENMITKLRESSLYLLYAPTDAFLLLLLGLLMRLVLLNYGIWHDRRSALKF 60
           MKLLSKYNSRENMITKLRESSLYLLYAPTDAFLLLLLGLLMRLVLLNYGIWHDRRSALKF
Sbjct: 405 MKLLSKYNSRENMITKLRESSLYLLYAPTDAFLLLLLGLLMRLVLLNYGIWHDRRSALKF 464

Query: 61  TDIDYFVFSDASKYVSIGMSPYMRDTYRYTPMLAILLLPTQYGFPSWGKYLFSISDLIAG 120
           TDIDYFVFSDASKYVSIGMSPYMRDTYRYTPMLAILLLPTQYGFPSWGKYLFSISDLIAG
Sbjct: 465 TDIDYFVFSDASKYVSIGMSPYMRDTYRYTPMLAILLLPTQYGFPSWGKYLFSISDLIAG 524

Query: 121 WLMIKLLSRRISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWL 180
           WLMIKLLSRRISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWL
Sbjct: 525 WLMIKLLSRRISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWL 584

Query: 181 ASLILGFSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSINQLKIVVGSLFMFTI 240
           ASLILGFSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSINQLKIVVGSLFMFTI
Sbjct: 585 ASLILGFSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSINQLKIVVGSLFMFTI 644

Query: 241 CNLLMYYLYGSPFLEHTYLYHFGRTDHRHNFSLHHLNLYYESSFGAKASSLFAFLPQLSL 300
           CNLLMYYLYGSPFLEHTYLYHFGRTDHRHNFSLHHLNLYYESSFGAKASSLFAFLPQLSL
Sbjct: 645 CNLLMYYLYGSPFLEHTYLYHFGRTDHRHNFSLHHLNLYYESSFGAKASSLFAFLPQLSL 704

Query: 301 CMLIPLVFGKKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKKGLIC 360
           CMLIPLVFGKKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKKGLIC
Sbjct: 705 CMLIPLVFGKKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKKGLIC 764

Query: 361 LSLWIIGQLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIILDSL 411
           LSLWIIGQLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIILDSL
Sbjct: 765 LSLWIIGQLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIILDSL 815
>ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase [Rattus norvegicus]
 dbj|BAB18566.1| (AB028126) mannosyltransferase [Rattus norvegicus]
          Length = 423

 Score =  514 bits (1310), Expect = e-144
 Identities = 156/401 (38%), Positives = 221/401 (54%), Gaps = 18/401 (4%)

Query: 22  LYLLYAPTDAFLLLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSP 81
           L L   P   F    +  L R+ L+ YG++ DR   +++TDIDY VF+DA+++V+ G SP
Sbjct: 12  LNLRVPPAGVF---GVAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSP 68

Query: 82  YMRDTYRYTPMLAILLLPTQYGFPSWGKYLFSISDLIAGW-LMIKLLSRRISYKRSLIYS 140
           Y+R TYRYTP+L+ LL P  Y    +GK+LF   DL+  + L   LL + +  +++  Y 
Sbjct: 69  YLRATYRYTPLLSWLLTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYC 128

Query: 141 SFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLASLILGFSVHFKIYPFMYGI 200
            FW+LNP    +S+RGN ++I+  L +  LYLIEK+ +  A++  GF+VH K+YP  Y +
Sbjct: 129 VFWLLNPLPMAVSSRGNADSIVASLVLTTLYLIEKRLIACAAVFYGFAVHMKMYPVTYIL 188

Query: 201 AFLVYFSKPKKGSTFM------------EKFLSLLSINQLKIVVGSLFMFTICNLLMYYL 248
              ++    +     +            +    L S   L  V  +   F   +   YY 
Sbjct: 189 PIALHLRPERDSDEGLRLARYSFQARLYDFLKRLCSWAVLLFVAIAGLTFLALSFGFYYK 248

Query: 249 YGSPFLEHTYLYHFGRTDHRHNFSLHHLNLYYES-SFGAKASSLFAFLPQLSLCMLIPLV 307
           YG  FLEHTYLYH  R D RHNFS +   LY  + S  +    + AFLPQ  L       
Sbjct: 249 YGWEFLEHTYLYHLTRRDIRHNFSPYFYMLYLTAESKWSFTLGIAAFLPQFILLSAASFA 308

Query: 308 FGKKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKKGLICLSLWIIG 367
           +  ++L    F  T  FVTFNKVCTSQYF+WYL  LPLV+P  ++  K+ ++ L LW IG
Sbjct: 309 Y-YRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLMLWFIG 367

Query: 368 QLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIIL 408
           Q LWL  AY LE  GK+ F+ +WL+GL F   N   L  I+
Sbjct: 368 QALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQII 408
>dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:1914806,
           evidence:ISS~phosphatidylinositol glycan, class
           M~putative [Mus musculus]
          Length = 423

 Score =  512 bits (1305), Expect = e-144
 Identities = 152/401 (37%), Positives = 218/401 (53%), Gaps = 18/401 (4%)

Query: 22  LYLLYAPTDAFLLLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSP 81
           L     P   F    +  L R+ L+ YG++ DR   +++TDIDY VF+DA+++V+ G SP
Sbjct: 12  LNFRVPPAGVF---GVAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSP 68

Query: 82  YMRDTYRYTPMLAILLLPTQYGFPSWGKYLFSISDLIAGW-LMIKLLSRRISYKRSLIYS 140
           Y+R TYRYTP+L+ LL P  Y    +GK+LF   DL+  + L   LL + +  +++  Y 
Sbjct: 69  YLRATYRYTPLLSWLLTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYC 128

Query: 141 SFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLASLILGFSVHFKIYPFMYGI 200
            FW+LNP    +S+RGN ++I+  L ++ LY IEK+ +  A++  GF+VH K+YP  Y +
Sbjct: 129 VFWLLNPLPMAVSSRGNADSIVASLVLSTLYFIEKRLIACAAVFYGFAVHMKMYPVTYIL 188

Query: 201 AFLVYFSKPKKGSTFM------------EKFLSLLSINQLKIVVGSLFMFTICNLLMYYL 248
              ++    +     +            +    L S   L  V  +   F   +   YY 
Sbjct: 189 PIALHLRPERDDDERLRQARFSFQARLYDFLRRLCSWAVLLFVAVAGLTFVALSFGFYYK 248

Query: 249 YGSPFLEHTYLYHFGRTDHRHNFSLHHLNLYYES-SFGAKASSLFAFLPQLSLCMLIPLV 307
           YG  FLEHTY YH  R D RHNFS +   LY  + S  +    + AFLPQ  L       
Sbjct: 249 YGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFTLGIAAFLPQFILLSAASFA 308

Query: 308 FGKKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKKGLICLSLWIIG 367
           +  ++L    F  T  FVTFNKVCTSQYF+WYL  LPLV+P  ++  K+ ++ L  W IG
Sbjct: 309 Y-YRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLLFWFIG 367

Query: 368 QLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIIL 408
           Q LWL  AY LE  GK+ F+ +WL+GL F   N   L  I+
Sbjct: 368 QALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQII 408
>gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mus musculus]
          Length = 423

 Score =  512 bits (1305), Expect = e-144
 Identities = 152/401 (37%), Positives = 218/401 (53%), Gaps = 18/401 (4%)

Query: 22  LYLLYAPTDAFLLLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSP 81
           L     P   F    +  L R+ L+ YG++ DR   +++TDIDY VF+DA+++V+ G SP
Sbjct: 12  LNFRVPPAGVF---GVAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSP 68

Query: 82  YMRDTYRYTPMLAILLLPTQYGFPSWGKYLFSISDLIAGW-LMIKLLSRRISYKRSLIYS 140
           Y+R TYRYTP+L+ LL P  Y    +GK+LF   DL+  + L   LL + +  +++  Y 
Sbjct: 69  YLRATYRYTPLLSWLLTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYC 128

Query: 141 SFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLASLILGFSVHFKIYPFMYGI 200
            FW+LNP    +S+RGN ++I+  L ++ LY IEK+ +  A++  GF+VH K+YP  Y +
Sbjct: 129 VFWLLNPLPMAVSSRGNADSIVASLVLSTLYFIEKRLIACAAVFYGFAVHMKMYPVTYIL 188

Query: 201 AFLVYFSKPKKGSTFM------------EKFLSLLSINQLKIVVGSLFMFTICNLLMYYL 248
              ++    +     +            +    L S   L  V  +   F   +   YY 
Sbjct: 189 PIALHLRPERDDDERLRHARFSFQARLYDFLRRLCSWAVLLFVAVAGLTFVALSFGFYYK 248

Query: 249 YGSPFLEHTYLYHFGRTDHRHNFSLHHLNLYYES-SFGAKASSLFAFLPQLSLCMLIPLV 307
           YG  FLEHTY YH  R D RHNFS +   LY  + S  +    + AFLPQ  L       
Sbjct: 249 YGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFTLGIAAFLPQFILLSAASFA 308

Query: 308 FGKKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKKGLICLSLWIIG 367
           +  ++L    F  T  FVTFNKVCTSQYF+WYL  LPLV+P  ++  K+ ++ L  W IG
Sbjct: 309 Y-YRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLLFWFIG 367

Query: 368 QLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIIL 408
           Q LWL  AY LE  GK+ F+ +WL+GL F   N   L  I+
Sbjct: 368 QALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQII 408
>ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosyltransferase [Homo
           sapiens]
 dbj|BAB18567.1| (AB028127) mannosyltransferase [Homo sapiens]
 gb|AAH19865.1|AAH19865 (BC019865) Unknown (protein for MGC:29896) [Homo sapiens]
          Length = 423

 Score =  511 bits (1304), Expect = e-144
 Identities = 157/401 (39%), Positives = 225/401 (55%), Gaps = 18/401 (4%)

Query: 22  LYLLYAPTDAFLLLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSP 81
           L L  AP   F    +  L R+ L+ YG++ DR   +++TDIDY VF+DA+++V+ G SP
Sbjct: 12  LNLKVAPAGVF---GVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSP 68

Query: 82  YMRDTYRYTPMLAILLLPTQYGFPSWGKYLFSISDLIAGW-LMIKLLSRRISYKRSLIYS 140
           Y+R TYRYTP+L  LL P  Y    +GK+LF   DL+  + L   LL + +  +++  Y 
Sbjct: 69  YLRATYRYTPLLGWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYC 128

Query: 141 SFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLASLILGFSVHFKIYPFMYGI 200
            FW+LNP    +S+RGN ++I+  L + +LYLI+K+ V  A++  GF+VH KIYP  Y +
Sbjct: 129 VFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMKIYPVTYIL 188

Query: 201 AFLVYFSKPKKGSTFMEKF------------LSLLSINQLKIVVGSLFMFTICNLLMYYL 248
              ++    +     + +F              L +   L  V  +   F   +   YY 
Sbjct: 189 PITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAVLLFVAVAGLTFFALSFGFYYE 248

Query: 249 YGSPFLEHTYLYHFGRTDHRHNFSLHHLNLYYES-SFGAKASSLFAFLPQLSLCMLIPLV 307
           YG  FLEHTY YH  R D RHNFS +   LY  + S  + +  + AFLPQL L   +   
Sbjct: 249 YGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLILLSAVSFA 308

Query: 308 FGKKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKKGLICLSLWIIG 367
           +  ++L    F  T  FVTFNKVCTSQYF+WYL  LPLV+P  ++  K+ ++ L LW IG
Sbjct: 309 Y-YRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLMLWFIG 367

Query: 368 QLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIIL 408
           Q +WL  AY LE  GK+ F+ +WL+GL F   N   L  I+
Sbjct: 368 QAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQII 408
>ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans]
 pir||T18769 hypothetical protein B0491.1 - Caenorhabditis elegans
 emb|CAA90090.1| (Z49907) B0491.1 [Caenorhabditis elegans]
          Length = 417

 Score =  472 bits (1203), Expect = e-132
 Identities = 140/391 (35%), Positives = 213/391 (53%), Gaps = 17/391 (4%)

Query: 34  LLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSPYMRDTYRYTPML 93
           +LL+  + R++L+ Y   HD    + FTDIDY VFSDA+K+VS G SP+ R TYRYTP L
Sbjct: 18  ILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPAL 77

Query: 94  AILLLPTQYGFPSWGKYLFSISDLIAGWLMIKLLSRRISYKRSLIYS-----------SF 142
           A +LLP  + FP +GK LF I D++   L  K++ + ++  +S                +
Sbjct: 78  AWILLPVVH-FPDFGKILFCIFDILVAILYFKIMEKDLNETKSETREEMKDDQTMNVVIY 136

Query: 143 WILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLASLILG-FSVHFKIYPFMYGIA 201
           W+ NP  AIIS RGN E+I+  + +  + L++K     A+L+ G  ++  KIYP +Y  +
Sbjct: 137 WLANPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIYPLIYLPS 196

Query: 202 FLVYFSKPKKGSTFMEKFLSLLS-INQLKIVVGSLFMFTICNLLMYYLYGSPFLEHTYLY 260
             +  S   + S  + KF SL+S       ++ +L  F    L  + +YG  FL+   +Y
Sbjct: 197 VFLSLSTIGEQSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLFFFQIYGQLFLDEYLIY 256

Query: 261 HFGRTDHRHNFSLHHLNLYY--ESSFGAKASSLFAFLPQLSLCMLIPLVFGKKNLPGTLF 318
           H  R D  HNFS +   LY    +   ++   L AF+PQ+ L +         +LP   F
Sbjct: 257 HVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLGAFIPQIVLIVFFAFKH-YDDLPFCWF 315

Query: 319 AQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKKGLICLSLWIIGQLLWLISAYNL 378
             TFAFVT+NKVCTSQYF+WY+V LPL+     +  +  L  ++ W   Q +WL++AY  
Sbjct: 316 ITTFAFVTYNKVCTSQYFVWYIVLLPLLAHKIMMSRQLALSLMAAWFATQGIWLLAAYLF 375

Query: 379 EMLGKSVFIPLWLSGLLFFFFNVYELKIILD 409
           E  G + F  ++L+  LF   N + LK I++
Sbjct: 376 EFQGWNTFFLMFLASCLFLIANSFILKQIIN 406
>dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei]
          Length = 430

 Score =  452 bits (1151), Expect = e-126
 Identities = 126/390 (32%), Positives = 201/390 (51%), Gaps = 26/390 (6%)

Query: 34  LLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSPYMRDTYRYTPML 93
           LLLLG L+RL+L+ Y  +HD+   +K+TDIDY +  D ++++  G SP+ R T+RYTP+L
Sbjct: 15  LLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRYTPLL 74

Query: 94  AILLLPTQYGFPSWGKYLFSISDLIAGWLMIKLLSRRISYKRSL--IYSSFWILNPFVAI 151
           A L++P+ +     GK +F+ SDL A W    +L +  + +RS   + S F + NP V  
Sbjct: 75  AALVMPSIWIANPMGKLIFASSDLGAAWYCYGVL-KSFAKERSAKWMVSLFILFNPIVLS 133

Query: 152 ISTRGNCEAILGILSIALLYLIEKKSVWLASLILGFSVHFKIYPFMYGIAFLV-YFSKPK 210
           +STRGN + ++  +S+ +L    ++  + A+ +LGF+VHFKIYP +Y +   +  + +  
Sbjct: 134 VSTRGNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIYALPLTLGVWEQSV 193

Query: 211 KGSTFMEKFLSLLSINQLKIVVGSLFMFTICNLLMYYLYGSPFLEHTYLYHFGRTDHRHN 270
             ST   + +   ++      + +   F +  +L Y  YG  +L   ++YH  R DHRHN
Sbjct: 194 AASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEAFIYHVYREDHRHN 253

Query: 271 FSLHHLNLYYESSFG------AKASSLFAFLPQLSLCMLIPLVFGKKNLPGTLFAQTFAF 324
           FS + L +Y   +          +  L AF+PQ  +   +     ++N       QT  F
Sbjct: 254 FSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKL-RRNTAHACCVQTVLF 312

Query: 325 VTFNKVCTSQYFMWYLVFLPLVLPNSKLL---------------SKKGLICLSLWIIGQL 369
           V FNKVCT QYF+W++ FL  +    K +                 K L  + +W     
Sbjct: 313 VAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKALGVVLMWAATIP 372

Query: 370 LWLISAYNLEMLGKSVFIPLWLSGLLFFFF 399
           LW+ +A  LE  G S F  LW+   LFF  
Sbjct: 373 LWVTTAVPLEFHGYSDFAKLWIVSCLFFLA 402
>emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Arabidopsis thaliana]
          Length = 417

 Score =  412 bits (1050), Expect = e-114
 Identities = 138/409 (33%), Positives = 206/409 (49%), Gaps = 71/409 (17%)

Query: 34  LLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSPYMRDTYRYTPML 93
           LL+  +L+R+ L+ YG W D    +++TD+DY VFSDA+  ++ G SPY R TYRY+P+L
Sbjct: 21  LLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYRYSPLL 80

Query: 94  AILLLPTQYGFPSWGKYLFSIS---DLIAGWLMIKLLSRRISYKRSLIYSSFWILNPFVA 150
           A+LL P  +   SWGK+LFS S         +   ++   IS     ++  +W       
Sbjct: 81  ALLLTPNSFFHRSWGKFLFSASVHQPHNVSMICFSVVLESIS-----MFQIYW------- 128

Query: 151 IISTRGNCEAILGILSIALLYLIEKKSVWLASLILGFSVHFKIYPFMYGIAFLVYFS--- 207
              + GN                    +  A+   G  VHF++YP +Y +  ++      
Sbjct: 129 ---SAGN--------------------LLQAAFWYGLVVHFRVYPIIYALPIILVLDTQV 165

Query: 208 ---------------KPKKGSTFMEK----------FLSLLSINQLKIVVGSLFMFTICN 242
                          + K  ++ ME+            SL S  ++   + S  +F  CN
Sbjct: 166 FRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALISGGVFLACN 225

Query: 243 LLMYYLYGSPFLEHTYLYHFGRTDHRHNFSLHHL--NLYYESSFGAKASSLFAFLPQLSL 300
            + +Y YG  FL    LYH  RTD RHNFS++     L+YE  F A    L +FLPQ ++
Sbjct: 226 AVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYERQFSA-VEKLISFLPQFTV 284

Query: 301 CMLIPLVFGKKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKL-LSKKGLI 359
              +   F  ++L   +F QT AFVTFNKV T+QYF+W+   LPL+LP S + L  +GL+
Sbjct: 285 QFALVFCF-SQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKLKWEGLL 343

Query: 360 CLSLWIIGQLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIIL 408
           C+ +WI  Q  WL+  Y LE  G +VF+PLW++ LLF   N + L  I+
Sbjct: 344 CIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFVLVRII 392
>ref|NP_012547.1| (NC_001142) Protein required for cell viability; Yjr013wp
           [Saccharomyces cerevisiae]
 sp|P47088|YJY3_YEAST HYPOTHETICAL 35.6 KD PROTEIN IN SPC1-ILV3 INTERGENIC REGION
 pir||S55201 hypothetical protein YJR013w - yeast (Saccharomyces cerevisiae)
 emb|CAA60935.1| (X87611) ORF YJR83.11 [Saccharomyces cerevisiae]
 emb|CAA89537.1| (Z49513) ORF YJR013w [Saccharomyces cerevisiae]
          Length = 305

 Score =  339 bits (862), Expect = 5e-92
 Identities = 126/296 (42%), Positives = 177/296 (59%), Gaps = 13/296 (4%)

Query: 124 IKLLSRRISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLASL 183
           +KLL++ IS KR+LI  S W+LNP V  ISTRGN E++L  L +  L+ ++K    LA +
Sbjct: 1   MKLLNQAISRKRALILESIWLLNPMVITISTRGNAESVLCCLIMFTLFFLQKSRYTLAGI 60

Query: 184 ILGFSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSINQLKIVVGSLFMFTICNL 243
           + G S+HFKIYP +Y I   ++    K+      +  SLL+I      + +L     C  
Sbjct: 61  LYGLSIHFKIYPIIYCIPIAIFIYYNKRNQGPRTQLTSLLNIG-----LSTLTTLLGCGW 115

Query: 244 LMYYLYGSPFLEHTYLYHFGRTDHRHNFSLHHLNLYYESS---FGAKASSLFAFLPQLSL 300
            MY +YG  FL+  YLYH  RTDHRHNFS+ ++ LY +S+    G    S +AF+PQL L
Sbjct: 116 AMYKIYGYEFLDQAYLYHLYRTDHRHNFSVWNMLLYLDSANKENGESNLSRYAFVPQLLL 175

Query: 301 CMLIPLVF----GKKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKK 356
            ++   +        NL   LF QTFAFVT+NKVCTSQYF+WYL+FLP  L  + +  KK
Sbjct: 176 VLVTGCLEWWNPTFDNLLRVLFVQTFAFVTYNKVCTSQYFVWYLIFLPFYLSRTHIGWKK 235

Query: 357 GLICLSLWIIGQLLWLISAYNLEMLGKSVFIP-LWLSGLLFFFFNVYELKIILDSL 411
           GL+  +LW+  Q +WL   Y LE  GK+VF P L+++ +LFF  NV+ L   +  +
Sbjct: 236 GLLMATLWVGTQGIWLSQGYYLEFEGKNVFYPGLFIASVLFFVTNVWLLGQFITDI 291
>ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster]
 gb|AAF46748.1| (AE003454) CG9865 gene product [Drosophila melanogaster]
 gb|AAK77251.1| (AY047519) GH02741p [Drosophila melanogaster]
          Length = 466

 Score =  293 bits (745), Expect = 2e-78
 Identities = 104/298 (34%), Positives = 154/298 (50%), Gaps = 23/298 (7%)

Query: 129 RRISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEK------KSVWL-- 180
             ++   +     FW+ NP  A+ISTRG+ +       I  +YL+ K      +S WL  
Sbjct: 158 ENLARASAC----FWLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHNVTRSYWLIF 213

Query: 181 -ASLILGFSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSIN--QLKIVVGSLFM 237
            A L  G  +H ++YP ++ +A+ +  S  +   T ++    +L  N  QL ++ G+L  
Sbjct: 214 GAGLAHGLVIHLRLYPLLFSLAYYLSLST-RLTQTPLDFLCQILRPNKQQLCLISGTLIS 272

Query: 238 FTICNLLMYYLYGSPFLEHTYLYHFGRTDHRHNFSLHHLNLYYESSFG-AKASSLFAFL- 295
                   Y +YG  ++   YLYHF R D RHNFSL  L  Y  S+   A+ S++   L 
Sbjct: 273 LVAFTWTFYTMYGWEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTLV 332

Query: 296 --PQLSLCMLIPLVFG--KKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSK 351
             PQ  L + + L FG  ++ LP  +FA  F  VT+N V TSQYF+WYL  LPL L N K
Sbjct: 333 LAPQFLLILYLSLSFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAILPLCLNNFK 392

Query: 352 LL-SKKGLICLSLWIIGQLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIIL 408
           +L  ++    L LW++ Q LWL+ AY LE    + F  + L G +FF  N Y L+ +L
Sbjct: 393 MLSWRRCFGLLFLWLLAQALWLLPAYLLEFHTWNTFYWIGLQGAVFFATNGYILEQLL 450
 Score =  136 bits (341), Expect = 7e-31
 Identities = 39/97 (40%), Positives = 61/97 (62%)

Query: 35  LLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSPYMRDTYRYTPMLA 94
           LL+  L+R+ L+ YG  HD +SA+ +TDIDY V +D ++ V  G +P+ R TYRY+P++A
Sbjct: 27  LLISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRYSPIMA 86

Query: 95  ILLLPTQYGFPSWGKYLFSISDLIAGWLMIKLLSRRI 131
            L        P+WGK L++  DL+   L+ +L+   I
Sbjct: 87  YLQTFNILLHPAWGKLLYATFDLLIATLIYRLVHMEI 123
>dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharomyces pombe]
          Length = 149

 Score =  170 bits (427), Expect = 5e-41
 Identities = 144/144 (100%), Positives = 144/144 (100%)

Query: 123 MIKLLSRRISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLAS 182
           MIKLLSRRISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLAS
Sbjct: 6   MIKLLSRRISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLAS 65

Query: 183 LILGFSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSINQLKIVVGSLFMFTICN 242
           LILGFSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSINQLKIVVGSLFMFTICN
Sbjct: 66  LILGFSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSINQLKIVVGSLFMFTICN 125

Query: 243 LLMYYLYGSPFLEHTYLYHFGRTD 266
           LLMYYLYGSPFLEHTYLYHFGRTD
Sbjct: 126 LLMYYLYGSPFLEHTYLYHFGRTD 149
>ref|NP_346994.1| (NC_003030) Uncharacterized membrane protein [Clostridium
           acetobutylicum]
 gb|AAK78334.1|AE007550_1 (AE007550) Uncharacterized membrane protein [Clostridium
           acetobutylicum]
          Length = 544

 Score =  108 bits (268), Expect = 2e-22
 Identities = 23/100 (23%), Positives = 50/100 (50%), Gaps = 2/100 (2%)

Query: 109 KYLFSISDLIAGWLMIKLLSRRISYKRSLIYSSFWILNPFVAIISTR-GNCEAILGILSI 167
           K    I+D+ + + + +L  +R SY+ S   + F++ NP + I ST  G  ++   +L I
Sbjct: 226 KLPSIIADIFSAYFVYRLSIKRFSYETSFALTLFYLFNPAILINSTLWGQADSFFTLLVI 285

Query: 168 ALLYLIEKKSVWLASLILGFSVHFKIYPFMYGIAFLVYFS 207
             +Y I +  +  +S+    SV  K    ++ +  ++++ 
Sbjct: 286 LAVYFISEDKIVPSSIFFALSVLMKPQGIIF-LPLVLFWY 324
>ref|NP_536724.1| (NM_080476) CDC91 cell division cycle 91-like 1 [Homo sapiens]
 emb|CAC14080.1| (AL118520) bA346K17.2 (A novel protein similar to the cell division
           control protein 91 (CDC91, YLR459W or L9122.2) from
           Yeast) [Homo sapiens]
          Length = 435

 Score = 36.8 bits (85), Expect = 0.63
 Identities = 40/239 (16%), Positives = 81/239 (33%), Gaps = 36/239 (15%)

Query: 68  FSDASKYVSIGMSPYMRDTYRYTPMLAILLLPTQYGFPSWGKYLFSISDLIAGWLMI--- 124
             +    + +G+SPY    +  TP++  L     +    + + +F I+D +    +    
Sbjct: 44  VVEGLSLLDLGVSPYSGAVFHETPLIIYLF----HFLIDYAELVFMITDALTAIALYFAI 99

Query: 125 -----------KLLSR---------RISYKRSLIY------SSFWILNPFVAIISTRGNC 158
                      KLL            +      +       + F++LNP+  +     + 
Sbjct: 100 QDFNKVVFKKQKLLLELDQYAPDVAELIRTPMEMRYIPLKVALFYLLNPYTILSCVAKST 159

Query: 159 EAILGILSIALLYLIEKKSVWLASLILGFSVHFKIYPFMYGIAFLVYFSKPK-KGSTFME 217
            AI   L    +    K S +L+++ L  + +  +YP    +  L+Y  + +        
Sbjct: 160 CAINNTLIAFFILTTIKGSAFLSAIFLALATYQSLYPLTLFVPGLLYLLQRQYIPVKMKS 219

Query: 218 KFLSLLSINQLKIVVGSLFMFTICNLLMYYLYGSPFLEHTYLYHFGRTDHRHNFSLHHL 276
           K   + S     + VGSL +    +   + L    F+   Y +     D   N  L   
Sbjct: 220 KAFWIFSWEYAMMYVGSLVVIICLS--FFLLSSWDFIPAVYGFILSVPDLTPNIGLFWY 276
>ref|NP_126953.1| (NC_000868) hypothetical protein [Pyrococcus abyssi]
 pir||B75036 hypothetical protein PAB1532 - Pyrococcus abyssi (strain Orsay)
 emb|CAB50183.1| (AJ248287) hypothetical protein [Pyrococcus abyssi]
          Length = 675

 Score = 34.1 bits (78), Expect = 4.9
 Identities = 34/184 (18%), Positives = 69/184 (37%), Gaps = 5/184 (2%)

Query: 60  FTDIDYFVFSDASKYVSIGMSPYMRDTYRYTPMLAILLLPTQYGFPS---WGKYLFSISD 116
           + D D   +   ++Y+++G  PY      + P+   LL      F      G+       
Sbjct: 30  YWDYDEGTYLLIARYINLGYLPYRDVLAVHPPLFYYLLALWLRVFGDSYIVGRLFSLSFG 89

Query: 117 LIAGWLMIKLLSRRISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKK 176
            ++  L  K+          +++S    L+P   I+++     +++    +  LYL  K 
Sbjct: 90  ALSLILAYKVGKIVRDEMLGVLFSLLLALDPQTIILNSTVFHASLIEFFVLLTLYLYLKG 149

Query: 177 SVWLASLILGFSVHFKIYPFMYGIAFLVY-FSKPKKGSTFMEKFLS-LLSINQLKIVVGS 234
              L + +LG     K     Y +   ++ +   K     +  + S LL+ +   IVV +
Sbjct: 150 KYLLMAFLLGVGTSVKHTFLPYAVGVFMFSWVGGKIKIKKLNFWKSLLLTYSTYLIVVYT 209

Query: 235 LFMF 238
           L   
Sbjct: 210 LISL 213
>pir||T37929 probable major facilitator protein - fission yeast
           (Schizosaccharomyces pombe)
          Length = 408

 Score = 33.3 bits (76), Expect = 7.6
 Identities = 44/278 (15%), Positives = 91/278 (31%), Gaps = 24/278 (8%)

Query: 77  IGMSPYMRDTYRYTPMLAIL-LLPTQYGFPSWGKYLFSISDLIAGWLMIKLLSRR----- 130
            G+ PY    +  +P+L IL       G  S  +++++    + G  +  +  +      
Sbjct: 57  NGLDPYSGGVFYQSPLLLILNYCCELLGGISVTRFVYTSISTMGGLFVYLIAKQARVLDP 116

Query: 131 ---ISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLASLILGF 187
              +S    L  S  ++LNP   +     + + IL   ++  +Y     S  + +  +  
Sbjct: 117 NQVLSTCSPLWISVIYLLNPLTFLPGIACSADMILNFTTLMTIYFASCGSYAIYACCMAL 176

Query: 188 SVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSINQLKIVVGSLFMFTICNLLMYY 247
           +V       +      +   K      F + F+           +  L + +      ++
Sbjct: 177 TVFINPNALLLFFPSYLILRKCNSSIKFRQIFV------VFLFYLAGLIITSG-----FF 225

Query: 248 LYGSPFLEHTYLYHFGRTDHRHNFSLHHLNLY-YESSFGAKASSLFAFLPQLSLCMLIPL 306
           L    FL+  +  +    D   N  L         + F      +FA LP L   + + +
Sbjct: 226 LNSLSFLKIPFRVYLDSHDLTPNLGLWWYFFTEMFNEFRTFFLFVFAILP-LMFVLPVSI 284

Query: 307 VFGKKNLPGTLFAQT--FAFVTFNKVCTSQYFMWYLVF 342
                 LP T+        F  +  +C    F+  L  
Sbjct: 285 RLYYLPLPITIALIGLHSLFKAYPSICDLSIFLSLLPI 322
CPU time:   136.69 user secs.	    2.34 sys. secs	  139.03 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.320    0.180    0.580 

Gapped
Lambda     K      H
   0.270   0.0630    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 327767031
Number of Sequences: 887402
Number of extensions: 19018649
Number of successful extensions: 61220
Number of sequences better than 10.0: 237
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 215
Number of HSP's that attempted gapping in prelim test: 60913
Number of HSP's gapped (non-prelim): 394
length of query: 411
length of database: 277,845,442
effective HSP length: 51
effective length of query: 360
effective length of database: 232,587,940
effective search space: 83731658400
effective search space used: 83731658400
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.0 bits)
S2: 75 (33.2 bits)