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GPI Anchor Biosynthesis Report: BAB18567.1 (PIG-M family, Homo sapiens)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (423 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 18 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosylt... 595 e-169
ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase ... 593 e-168
dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:19148... 592 e-168
gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mu... 590 e-167
ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans] ... 471 e-131
emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [S... 463 e-129
emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Ara... 455 e-127
dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei] 446 e-124
ref|NP_012547.1| (NC_001142) Protein required for cell viability... 328 1e-88
ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster] >g... 297 3e-79
dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharom... 161 2e-38
ref|NP_346994.1| (NC_003030) Uncharacterized membrane protein [C... 112 2e-23
Sequences with E-value WORSE than threshold

ref|NP_176499.1| (NM_104989) unknown protein [Arabidopsis thalia... 36 1.3
ref|NP_536724.1| (NM_080476) CDC91 cell division cycle 91-like 1... 35 2.6
ref|NP_563912.1| (NM_101143) expressed protein [Arabidopsis thal... 34 3.5
ref|NP_488389.1| (NC_003272) unknown protein [Nostoc sp. PCC 712... 34 3.5
pir||T37929 probable major facilitator protein - fission yeast ... 33 8.5
Alignments
>ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosyltransferase [Homo
           sapiens]
 dbj|BAB18567.1| (AB028127) mannosyltransferase [Homo sapiens]
 gb|AAH19865.1|AAH19865 (BC019865) Unknown (protein for MGC:29896) [Homo sapiens]
          Length = 423

 Score =  595 bits (1519), Expect = e-169
 Identities = 423/423 (100%), Positives = 423/423 (100%)

Query: 1   MGSTKHWGEWLLNLKVAPAGVFGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAAR 60
           MGSTKHWGEWLLNLKVAPAGVFGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAAR
Sbjct: 1   MGSTKHWGEWLLNLKVAPAGVFGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAAR 60

Query: 61  FVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLG 120
           FVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLG
Sbjct: 61  FVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLG 120

Query: 121 RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMK 180
           RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMK
Sbjct: 121 RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMK 180

Query: 181 IYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAVLLFVAVAGLTFFA 240
           IYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAVLLFVAVAGLTFFA
Sbjct: 181 IYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAVLLFVAVAGLTFFA 240

Query: 241 LSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLI 300
           LSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLI
Sbjct: 241 LSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLI 300

Query: 301 LLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL 360
           LLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL
Sbjct: 301 LLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL 360

Query: 361 LMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEEPLTERI 420
           LMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEEPLTERI
Sbjct: 361 LMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEEPLTERI 420

Query: 421 KYD 423
           KYD
Sbjct: 421 KYD 423
>ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase [Rattus norvegicus]
 dbj|BAB18566.1| (AB028126) mannosyltransferase [Rattus norvegicus]
          Length = 423

 Score =  593 bits (1515), Expect = e-168
 Identities = 385/423 (91%), Positives = 401/423 (94%)

Query: 1   MGSTKHWGEWLLNLKVAPAGVFGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAAR 60
           M  TKHWGEW LNL+V PAGVFGVAFLARVALVFYGVFQDRTL VRYTDIDY VFTDAAR
Sbjct: 1   MSYTKHWGEWFLNLRVPPAGVFGVAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAAR 60

Query: 61  FVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLG 120
           FVTEGRSPYLRATYRYTPLL WLLTPN+YLSELFGKFLFISCDLLTAFLLYRLLLLKGLG
Sbjct: 61  FVTEGRSPYLRATYRYTPLLSWLLTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLG 120

Query: 121 RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMK 180
           RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVL  LYLI+KRL+ACAAVFYGFAVHMK
Sbjct: 121 RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLTTLYLIEKRLIACAAVFYGFAVHMK 180

Query: 181 IYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAVLLFVAVAGLTFFA 240
           +YPVTYILPI LHL P+RD+D+ LR  RY+FQA LY+ LKRLC+ AVLLFVA+AGLTF A
Sbjct: 181 MYPVTYILPIALHLRPERDSDEGLRLARYSFQARLYDFLKRLCSWAVLLFVAIAGLTFLA 240

Query: 241 LSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLI 300
           LSFGFYY+YGWEFLEHTY YHLTRRDIRHNFSPYFYMLYLTAESKWSF+LGIAAFLPQ I
Sbjct: 241 LSFGFYYKYGWEFLEHTYLYHLTRRDIRHNFSPYFYMLYLTAESKWSFTLGIAAFLPQFI 300

Query: 301 LLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL 360
           LLSA SFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL
Sbjct: 301 LLSAASFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL 360

Query: 361 LMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEEPLTERI 420
           LMLWFIGQA+WLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKE+ LTERI
Sbjct: 361 LMLWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEDRLTERI 420

Query: 421 KYD 423
           KYD
Sbjct: 421 KYD 423
>dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:1914806,
           evidence:ISS~phosphatidylinositol glycan, class
           M~putative [Mus musculus]
          Length = 423

 Score =  592 bits (1511), Expect = e-168
 Identities = 380/423 (89%), Positives = 399/423 (93%)

Query: 1   MGSTKHWGEWLLNLKVAPAGVFGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAAR 60
           M    HWGEW+LN +V PAGVFGVAFLARVALVFYGVFQDRTL VRYTDIDY VFTDAAR
Sbjct: 1   MSYPMHWGEWILNFRVPPAGVFGVAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAAR 60

Query: 61  FVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLG 120
           FVTEGRSPYLRATYRYTPLL WLLTPN+YLSELFGKFLFISCDLLTAFLLYRLLLLKGLG
Sbjct: 61  FVTEGRSPYLRATYRYTPLLSWLLTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLG 120

Query: 121 RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMK 180
           RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVL  LY I+KRL+ACAAVFYGFAVHMK
Sbjct: 121 RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLSTLYFIEKRLIACAAVFYGFAVHMK 180

Query: 181 IYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAVLLFVAVAGLTFFA 240
           +YPVTYILPI LHL P+RD+D+ LRQ R++FQA LY+ L+RLC+ AVLLFVAVAGLTF A
Sbjct: 181 MYPVTYILPIALHLRPERDDDERLRQARFSFQARLYDFLRRLCSWAVLLFVAVAGLTFVA 240

Query: 241 LSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLI 300
           LSFGFYY+YGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSF+LGIAAFLPQ I
Sbjct: 241 LSFGFYYKYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFTLGIAAFLPQFI 300

Query: 301 LLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL 360
           LLSA SFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL
Sbjct: 301 LLSAASFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL 360

Query: 361 LMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEEPLTERI 420
           L+ WFIGQA+WLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKE+ LTERI
Sbjct: 361 LLFWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEDRLTERI 420

Query: 421 KYD 423
           KYD
Sbjct: 421 KYD 423
>gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mus musculus]
          Length = 423

 Score =  590 bits (1506), Expect = e-167
 Identities = 379/423 (89%), Positives = 398/423 (93%)

Query: 1   MGSTKHWGEWLLNLKVAPAGVFGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAAR 60
           M    HWGEW+LN +V PAGVFGVAFLARVALVFYGVFQDRTL VRYTDIDY VFTDAAR
Sbjct: 1   MSYPMHWGEWILNFRVPPAGVFGVAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAAR 60

Query: 61  FVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLG 120
           FVTEGRSPYLRATYRYTPLL WLLTPN+YLSELFGKFLFISCDLLTAFLLYRLLLLKGLG
Sbjct: 61  FVTEGRSPYLRATYRYTPLLSWLLTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLG 120

Query: 121 RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMK 180
           RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVL  LY I+KRL+ACAAVFYGFAVHMK
Sbjct: 121 RRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLSTLYFIEKRLIACAAVFYGFAVHMK 180

Query: 181 IYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAVLLFVAVAGLTFFA 240
           +YPVTYILPI LHL P+RD+D+ LR  R++FQA LY+ L+RLC+ AVLLFVAVAGLTF A
Sbjct: 181 MYPVTYILPIALHLRPERDDDERLRHARFSFQARLYDFLRRLCSWAVLLFVAVAGLTFVA 240

Query: 241 LSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLI 300
           LSFGFYY+YGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSF+LGIAAFLPQ I
Sbjct: 241 LSFGFYYKYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFTLGIAAFLPQFI 300

Query: 301 LLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL 360
           LLSA SFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL
Sbjct: 301 LLSAASFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVL 360

Query: 361 LMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEEPLTERI 420
           L+ WFIGQA+WLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKE+ LTERI
Sbjct: 361 LLFWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEDRLTERI 420

Query: 421 KYD 423
           KYD
Sbjct: 421 KYD 423
>ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans]
 pir||T18769 hypothetical protein B0491.1 - Caenorhabditis elegans
 emb|CAA90090.1| (Z49907) B0491.1 [Caenorhabditis elegans]
          Length = 417

 Score =  471 bits (1201), Expect = e-131
 Identities = 168/404 (41%), Positives = 240/404 (58%), Gaps = 26/404 (6%)

Query: 21  VFGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLL 80
           +  VAF+AR+ LVFY    D    V +TDIDY VF+DAA+ V+ G SP+ RATYRYTP L
Sbjct: 18  ILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPAL 77

Query: 81  GWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLL----------LLKGLGRRQACGYCVF 130
            W+L P ++  + FGK LF   D+L A L ++++            + +   Q     ++
Sbjct: 78  AWILLPVVHFPD-FGKILFCIFDILVAILYFKIMEKDLNETKSETREEMKDDQTMNVVIY 136

Query: 131 WLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYG-FAVHMKIYPVTYILP 189
           WL NPL   +S+RGNA+SIVA++VL+ + L++K     AA+ +G  A+ +KIYP+ Y+  
Sbjct: 137 WLANPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIYPLIYLPS 196

Query: 190 ITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLC-NRAVLLFVAVAGLTFFALSFGFYYE 248
           + L L    +            Q+C+    K L  N     ++ V   +F A+   F+  
Sbjct: 197 VFLSLSTIGE------------QSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLFFFQI 244

Query: 249 YGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYL-TAESKWSFSLGIAAFLPQLILLSAVSF 307
           YG  FL+    YH+ RRD+ HNFSPYFY+LYL  A    S  +G+ AF+PQ++L+   +F
Sbjct: 245 YGQLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLGAFIPQIVLIVFFAF 304

Query: 308 AYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLMLWFIG 367
            +Y DL FC F+ T  FVT+NKVCTSQYF+WY+ LLPL+   + M  + A+ L+  WF  
Sbjct: 305 KHYDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLLAHKIMMSRQLALSLMAAWFAT 364

Query: 368 QAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHY 411
           Q +WL  AY+ EFQG NTF  ++LA   FL+ N  IL QII+HY
Sbjct: 365 QGIWLLAAYLFEFQGWNTFFLMFLASCLFLIANSFILKQIINHY 408
>emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [Schizosaccharomyces
           pombe]
          Length = 815

 Score =  463 bits (1181), Expect = e-129
 Identities = 155/401 (38%), Positives = 224/401 (55%), Gaps = 18/401 (4%)

Query: 12  LNLKVAPAGVFGVAFLARVALVF---YGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSP 68
           L L  AP   F +  L  +  +    YG++ DR   +++TDIDY VF+DA+++V+ G SP
Sbjct: 426 LYLLYAPTDAFLLLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSP 485

Query: 69  YLRATYRYTPLLGWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYC 128
           Y+R TYRYTP+L  LL P  Y    +GK+LF   DL+  +L+ +LL    +  +++  Y 
Sbjct: 486 YMRDTYRYTPMLAILLLPTQYGFPSWGKYLFSISDLIAGWLMIKLLSR-RISYKRSLIYS 544

Query: 129 VFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMKIYPVTYIL 188
            FW+LNP    +S+RGN ++I+  L + +LYLI+K+ V  A++  GF+VH KIYP  Y +
Sbjct: 545 SFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLASLILGFSVHFKIYPFMYGI 604

Query: 189 PITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAVLLFVAVAGLTFFALSFGFYYE 248
              ++    +     + +F              L +   L  V  +   F   +   YY 
Sbjct: 605 AFLVYFSKPKKGSTFMEKFL------------SLLSINQLKIVVGSLFMFTICNLLMYYL 652

Query: 249 YGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLILLSAVSFA 308
           YG  FLEHTY YH  R D RHNFS +   LY  + S  + +  + AFLPQL L   +   
Sbjct: 653 YGSPFLEHTYLYHFGRTDHRHNFSLHHLNLYYES-SFGAKASSLFAFLPQLSLCMLIPLV 711

Query: 309 Y-YRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLMLWFIG 367
           +  ++L    F  T  FVTFNKVCTSQYF+WYL  LPLV+P  ++  K+ ++ L LW IG
Sbjct: 712 FGKKNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKKGLICLSLWIIG 771

Query: 368 QAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQII 408
           Q +WL  AY LE  GK+ F+ +WL+GL F   N   L  I+
Sbjct: 772 QLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIIL 812
>emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Arabidopsis thaliana]
          Length = 417

 Score =  455 bits (1159), Expect = e-127
 Identities = 156/415 (37%), Positives = 214/415 (50%), Gaps = 50/415 (12%)

Query: 22  FGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLG 81
              + L RV L+ YG +QD  + VRYTD+DY VF+DAA  +  G SPY R TYRY+PLL 
Sbjct: 22  LVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYRYSPLLA 81

Query: 82  WLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVS 141
            LLTPN +    +GKFLF +                     Q     +            
Sbjct: 82  LLLTPNSFFHRSWGKFLFSA------------------SVHQPHNVSMICF--------- 114

Query: 142 SRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPD--RD 199
                  ++ S+ +  +Y     L+  AA +YG  VH ++YP+ Y LPI L L     R 
Sbjct: 115 -----SVVLESISMFQIYWSAGNLL-QAAFWYGLVVHFRVYPIIYALPIILVLDTQVFRS 168

Query: 200 NDKSLRQF--------------RYTFQACLYELLKRLCNRAVLLFVAVAGLTFFALSFGF 245
             K    +              R TF   L   LK L +R  ++F  ++G  F A +   
Sbjct: 169 GQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALISGGVFLACNAVS 228

Query: 246 YYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLILLSAV 305
           +Y YG EFL     YHLTR D RHNFS YFY +YL  E ++S    + +FLPQ  +  A+
Sbjct: 229 FYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYERQFSAVEKLISFLPQFTVQFAL 288

Query: 306 SFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWK-RAVVLLMLW 364
            F + +DLVFC FL T  FVTFNKV T+QYF+W+ CLLPL++P   M  K   ++ +++W
Sbjct: 289 VFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKLKWEGLLCIIMW 348

Query: 365 FIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEEPLTER 419
              Q  WL   Y+LEF+G N FL +W+A L FL  N  +L++II  ++  PL  R
Sbjct: 349 IGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFVLVRIIQRHRFSPLFRR 403
>dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei]
          Length = 430

 Score =  446 bits (1136), Expect = e-124
 Identities = 135/400 (33%), Positives = 209/400 (51%), Gaps = 35/400 (8%)

Query: 22  FGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLG 81
             +  L R+ L+ Y  F D+   V+YTDIDY +  D AR +  G SP+ R T+RYTPLL 
Sbjct: 16  LLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRYTPLLA 75

Query: 82  WLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVS 141
            L+ P+I+++   GK +F S DL  A+  Y +L      R       +F L NP+ ++VS
Sbjct: 76  ALVMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWMVSLFILFNPIVLSVS 135

Query: 142 SRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPDRDND 201
           +RGN+D +V  + LMVL    +R    AA   GFAVH KIYP+ Y LP+TL +      +
Sbjct: 136 TRGNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIYALPLTLGVW-----E 190

Query: 202 KSLRQFRYTFQACLYELLKRLCNRAVLLFVA--VAGLTFFALSFGFYYEYGWEFLEHTYF 259
           +S+     T+        +R+   AV++ +   +A ++F   +   Y +YG ++L   + 
Sbjct: 191 QSVAASTNTW--------RRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEAFI 242

Query: 260 YHLTRRDIRHNFSPYFYMLYLTAESKW-----SFSLGIAAFLPQLILLSAVSFAYYRDLV 314
           YH+ R D RHNFSPY+ ++YL    +       FS  + AF+PQ ++LS VS+   R+  
Sbjct: 243 YHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKLRRNTA 302

Query: 315 FCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVM---------------PLVRMPWKRAVV 359
             C + T +FV FNKVCT QYF+W++  L  +                      W +A+ 
Sbjct: 303 HACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKALG 362

Query: 360 LLMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLI 399
           ++++W     +W+  A  LEF G + F  +W+    F L 
Sbjct: 363 VVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLA 402
>ref|NP_012547.1| (NC_001142) Protein required for cell viability; Yjr013wp
           [Saccharomyces cerevisiae]
 sp|P47088|YJY3_YEAST HYPOTHETICAL 35.6 KD PROTEIN IN SPC1-ILV3 INTERGENIC REGION
 pir||S55201 hypothetical protein YJR013w - yeast (Saccharomyces cerevisiae)
 emb|CAA60935.1| (X87611) ORF YJR83.11 [Saccharomyces cerevisiae]
 emb|CAA89537.1| (Z49513) ORF YJR013w [Saccharomyces cerevisiae]
          Length = 305

 Score =  328 bits (833), Expect = 1e-88
 Identities = 103/307 (33%), Positives = 162/307 (52%), Gaps = 25/307 (8%)

Query: 114 LLLKGLGRRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFY 173
           LL + + R++A      WLLNP+ + +S+RGNA+S++  L++  L+ ++K     A + Y
Sbjct: 3   LLNQAISRKRALILESIWLLNPMVITISTRGNAESVLCCLIMFTLFFLQKSRYTLAGILY 62

Query: 174 GFAVHMKIYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAVLLFVAV 233
           G ++H KIYP+ Y +PI + +  ++ N     Q        L                  
Sbjct: 63  GLSIHFKIYPIIYCIPIAIFIYYNKRNQGPRTQLTSLLNIGLS----------------- 105

Query: 234 AGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWS--FSLG 291
              T     +  Y  YG+EFL+  Y YHL R D RHNFS +  +LYL + +K +   +L 
Sbjct: 106 TLTTLLGCGWAMYKIYGYEFLDQAYLYHLYRTDHRHNFSVWNMLLYLDSANKENGESNLS 165

Query: 292 IAAFLPQLILLSAVSF-----AYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLV 346
             AF+PQL+L+            + +L+   F+ T  FVT+NKVCTSQYF+WYL  LP  
Sbjct: 166 RYAFVPQLLLVLVTGCLEWWNPTFDNLLRVLFVQTFAFVTYNKVCTSQYFVWYLIFLPFY 225

Query: 347 MPLVRMPWKRAVVLLMLWFIGQAMWLAPAYVLEFQGKNTFLF-IWLAGLFFLLINCSILI 405
           +    + WK+ +++  LW   Q +WL+  Y LEF+GKN F   +++A + F + N  +L 
Sbjct: 226 LSRTHIGWKKGLLMATLWVGTQGIWLSQGYYLEFEGKNVFYPGLFIASVLFFVTNVWLLG 285

Query: 406 QIISHYK 412
           Q I+  K
Sbjct: 286 QFITDIK 292
>ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster]
 gb|AAF46748.1| (AE003454) CG9865 gene product [Drosophila melanogaster]
 gb|AAK77251.1| (AY047519) GH02741p [Drosophila melanogaster]
          Length = 466

 Score =  297 bits (754), Expect = 3e-79
 Identities = 114/315 (36%), Positives = 164/315 (51%), Gaps = 31/315 (9%)

Query: 115 LLKGLGRRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKR---------L 165
             + L R  AC    FWL NPL   +S+RG+ D   +  V++ +YL+ K          L
Sbjct: 156 HPENLARASAC----FWLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHNVTRSYWL 211

Query: 166 VACAAVFYGFAVHMKIYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNR 225
           +  A + +G  +H+++YP+ + L   L L         L Q    F   L ++L+   N+
Sbjct: 212 IFGAGLAHGLVIHLRLYPLLFSLAYYLSLST------RLTQTPLDF---LCQILRP--NK 260

Query: 226 AVLLFVAVAGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAES- 284
             L  ++   ++  A ++ FY  YGWE++   Y YH  R+D RHNFS  F + YL + S 
Sbjct: 261 QQLCLISGTLISLVAFTWTFYTMYGWEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASS 320

Query: 285 --KWSFSLGIAAFLPQL--ILLSAVSFAYYRD-LVFCCFLHTSIFVTFNKVCTSQYFLWY 339
             + S  L      PQ   IL  ++SF  +R  L FC F    + VT+N V TSQYF+WY
Sbjct: 321 VAEPSAILKTLVLAPQFLLILYLSLSFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWY 380

Query: 340 LCLLPLVMPLVRM-PWKRAVVLLMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLL 398
           L +LPL +   +M  W+R   LL LW + QA+WL PAY+LEF   NTF +I L G  F  
Sbjct: 381 LAILPLCLNNFKMLSWRRCFGLLFLWLLAQALWLLPAYLLEFHTWNTFYWIGLQGAVFFA 440

Query: 399 INCSILIQIISHYKE 413
            N  IL Q++SHY  
Sbjct: 441 TNGYILEQLLSHYGF 455
 Score =  134 bits (337), Expect = 2e-30
 Identities = 45/103 (43%), Positives = 64/103 (61%)

Query: 22  FGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLG 81
             ++ L R+AL+ YG   D    V YTDIDY+V TD AR V  G +P+ R TYRY+P++ 
Sbjct: 27  LLISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRYSPIMA 86

Query: 82  WLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQA 124
           +L T NI L   +GK L+ + DLL A L+YRL+ ++   + Q 
Sbjct: 87  YLQTFNILLHPAWGKLLYATFDLLIATLIYRLVHMEIKSQYQK 129
>dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharomyces pombe]
          Length = 149

 Score =  161 bits (405), Expect = 2e-38
 Identities = 49/159 (30%), Positives = 78/159 (48%), Gaps = 12/159 (7%)

Query: 108 FLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVA 167
           ++    LL + +  +++  Y  FW+LNP    +S+RGN ++I+  L + +LYLI+K+ V 
Sbjct: 3   WVAMIKLLSRRISYKRSLIYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVW 62

Query: 168 CAAVFYGFAVHMKIYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAV 227
            A++  GF+VH KIYP  Y +   ++    +     + +F              L +   
Sbjct: 63  LASLILGFSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFL------------SLLSINQ 110

Query: 228 LLFVAVAGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRD 266
           L  V  +   F   +   YY YG  FLEHTY YH  R D
Sbjct: 111 LKIVVGSLFMFTICNLLMYYLYGSPFLEHTYLYHFGRTD 149
>ref|NP_346994.1| (NC_003030) Uncharacterized membrane protein [Clostridium
           acetobutylicum]
 gb|AAK78334.1|AE007550_1 (AE007550) Uncharacterized membrane protein [Clostridium
           acetobutylicum]
          Length = 544

 Score =  112 bits (278), Expect = 2e-23
 Identities = 31/113 (27%), Positives = 56/113 (49%), Gaps = 3/113 (2%)

Query: 96  KFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSR-GNADSIVASLV 154
           K   I  D+ +A+ +YRL  +K      +    +F+L NP  +  S+  G ADS    LV
Sbjct: 226 KLPSIIADIFSAYFVYRL-SIKRFSYETSFALTLFYLFNPAILINSTLWGQADSFFTLLV 284

Query: 155 LMVLYLIKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPDRDNDKSLRQF 207
           ++ +Y I +  +  +++F+  +V MK   + + LP+ L       N KS+ + 
Sbjct: 285 ILAVYFISEDKIVPSSIFFALSVLMKPQGIIF-LPLVLFWYIKNKNLKSIFKS 336
>ref|NP_176499.1| (NM_104989) unknown protein [Arabidopsis thaliana]
 gb|AAG51607.1|AC010795_11 (AC010795) unknown protein; 55290-58984 [Arabidopsis thaliana]
          Length = 551

 Score = 36.1 bits (83), Expect = 1.3
 Identities = 38/171 (22%), Positives = 65/171 (37%), Gaps = 21/171 (12%)

Query: 126 GYCVFWLLNPLPMAVSSRGNADSIVASL-VLMVLYLIKKRLVACAAVFYGFAVHMKIY-- 182
              + +L NP    VS  G + S + +L V++ L+    R V  AA     A H+ +Y  
Sbjct: 267 IAALVYLWNPFT-IVSCVGLSTSPIENLAVILALFGAVTRRVPLAAFGLVIATHLSLYPA 325

Query: 183 ----PVTYIL-------PITLHLLPDRDNDKSLRQFRYTFQACLYELLKRLCNRAVLL-- 229
               P+ ++L       PI L L      ++       + QA L +          +   
Sbjct: 326 TLTIPIIFLLGYGLDAPPIKLFLQTRSVENEESSTSTVSKQAKLKQTTHLPFLWKTVAHF 385

Query: 230 --FVAVAGLTFFALSFGFYYEYG--WEFLEHTYFYHLTRRDIRHNFSPYFY 276
             +V +  L    L      +YG   E  + TY + L+  D+  N   ++Y
Sbjct: 386 LFWVLLWSLYVLILCALSLNKYGGLEEMFKRTYGFILSIEDLSPNIGVFWY 436
>ref|NP_536724.1| (NM_080476) CDC91 cell division cycle 91-like 1 [Homo sapiens]
 emb|CAC14080.1| (AL118520) bA346K17.2 (A novel protein similar to the cell division
           control protein 91 (CDC91, YLR459W or L9122.2) from
           Yeast) [Homo sapiens]
          Length = 435

 Score = 34.9 bits (80), Expect = 2.6
 Identities = 61/331 (18%), Positives = 110/331 (32%), Gaps = 52/331 (15%)

Query: 55  FTDAARFVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLL 114
             +    +  G SPY  A +  TPL+ +L    I  +E     +F+  D LTA  LY  +
Sbjct: 44  VVEGLSLLDLGVSPYSGAVFHETPLIIYLFHFLIDYAE----LVFMITDALTAIALYFAI 99

Query: 115 LLKGL----------------------------GRRQACGYCVFWLLNPLPMAVSSRGNA 146
                                             R       +F+LLNP  +      + 
Sbjct: 100 QDFNKVVFKKQKLLLELDQYAPDVAELIRTPMEMRYIPLKVALFYLLNPYTILSCVAKST 159

Query: 147 DSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPDRDNDKSLRQ 206
            +I  +L+   +    K     +A+F   A +  +YP+T  +P  L+L            
Sbjct: 160 CAINNTLIAFFILTTIKGSAFLSAIFLALATYQSLYPLTLFVPGLLYL-----------L 208

Query: 207 FRYTFQACLYELLKRLCNRAVLLFVAVAGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRD 266
            R      +      + +    +    + +    LS  F+    W+F+   Y + L+  D
Sbjct: 209 QRQYIPVKMKSKAFWIFSWEYAMMYVGSLVVIICLS--FFLLSSWDFIPAVYGFILSVPD 266

Query: 267 IRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLILLSA---VSFAYYRDLVFCCFLHTSI 323
           +  N   ++   Y  AE    FSL     + Q+ +      ++       +F  F+  ++
Sbjct: 267 LTPNIGLFW---YFFAEMFEHFSL-FFVCVFQINVFFYTIPLAIKLKEHPIFFMFIQIAV 322

Query: 324 FVTFNKVCTSQYFLWYLCLLPLVMPLVRMPW 354
              F    T      Y+   P+   L R   
Sbjct: 323 IAIFKSYPTVGDVALYMAFFPVWNHLYRFLR 353
>ref|NP_563912.1| (NM_101143) expressed protein [Arabidopsis thaliana]
 gb|AAK44067.1|AF370252_1 (AF370252) unknown protein [Arabidopsis thaliana]
 gb|AAL34242.1| (AY063068) unknown protein [Arabidopsis thaliana]
          Length = 474

 Score = 34.5 bits (79), Expect = 3.5
 Identities = 65/311 (20%), Positives = 108/311 (33%), Gaps = 48/311 (15%)

Query: 67  SPYLRATYRYTPLLGWLLTPNIY------LSELFGKFLFISCDLLTAFLLYRLLLLKGLG 120
           SPY  + Y  +PLL  +L P          S      +F+  DLL+A LL  +       
Sbjct: 70  SPYAGSMYHGSPLLLSILGPLTVQRIEGQSSHHLCSLVFVIADLLSALLLRAIGQKLQKS 129

Query: 121 RR---QACGY----------------CVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLI 161
            R   +  G                  + +L NP  +      +   I    V++ LY  
Sbjct: 130 YRMNLRPLGVLRSSQDRGILPCGDIAALVYLWNPFTILSCVGLSTSPIENLAVIVSLYGA 189

Query: 162 KKRLVACAAVFYGFAVHMKIY------PVTYILPITLHLLPDRDNDKSLRQFRYTFQACL 215
             R V  AA     A H+ +Y      P+ ++L   L   P +   ++      T     
Sbjct: 190 VTRRVPLAAFGLIMATHLSLYSAPLVFPLIFLLGYGLDAPPIKKFLQTRHGGVETGSTSD 249

Query: 216 YELLKRLCNRAVLLFVAVAGLTFF--ALSFGFY---------YEYG--WEFLEHTYFYHL 262
               ++L     L F     L F    L +  Y          +YG   E  + TY + L
Sbjct: 250 VSKQEKLTPTTQLPFSWRTVLHFVFWVLVWSSYVLVLCSLSLKQYGGLEEMFKRTYGFIL 309

Query: 263 TRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQLILLSAVSFAYYRDLVFCCFLHTS 322
              D+  N   ++Y  +     ++S +  +  F    ILL+++    +R     CFL   
Sbjct: 310 RIEDLSPNIGVFWY--FFAEAFEFSRNYLLIIF-NLYILLTSIPSLMFRLKHRPCFL-AF 365

Query: 323 IFVTFNKVCTS 333
            ++ F  +  S
Sbjct: 366 AYLAFTSILKS 376
>ref|NP_488389.1| (NC_003272) unknown protein [Nostoc sp. PCC 7120]
 dbj|BAB76048.1| (AP003596) ORF_ID:all4349~unknown protein [Nostoc sp. PCC 7120]
          Length = 399

 Score = 34.5 bits (79), Expect = 3.5
 Identities = 42/167 (25%), Positives = 76/167 (45%), Gaps = 9/167 (5%)

Query: 41  RTLHVRYTDIDYQVFTDA--ARFVTEGRSPYLRATYR-YT-PLLGWLLTPNIYLSELFG- 95
           R + V    +DY+ F        V+ G    L+ ++  YT P L W+L     LS L   
Sbjct: 43  RLICVPNKSVDYKYFLAPWYDFIVSNGGFSALKYSFADYTPPYLYWILIAGTVLSGLPKI 102

Query: 96  ---KFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSR-GNADSIVA 151
              K   +S D + AF  Y+++ LK    R+A    +  +L+P+ +  S+  G  DSI  
Sbjct: 103 LAIKLFAMSVDFVCAFFTYKIVKLKYPEGRKAIFAFITVILSPVVIYNSALWGQCDSIYT 162

Query: 152 SLVLMVLYLIKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPDR 198
           + ++  +Y +  +    A V +G A+  K+  +     + + L+ +R
Sbjct: 163 TGLIACIYFLSIQKQLSALVSFGIALSFKLQAMFLSPLLLIFLIKNR 209
>pir||T37929 probable major facilitator protein - fission yeast
           (Schizosaccharomyces pombe)
          Length = 408

 Score = 33.3 bits (76), Expect = 8.5
 Identities = 31/152 (20%), Positives = 49/152 (31%), Gaps = 8/152 (5%)

Query: 64  EGRSPYLRATYRYTPLLGWL-LTPNIYLSELFGKFLFISCDLLTAFLLYRLLL------- 115
            G  PY    +  +PLL  L     +       +F++ S   +    +Y +         
Sbjct: 57  NGLDPYSGGVFYQSPLLLILNYCCELLGGISVTRFVYTSISTMGGLFVYLIAKQARVLDP 116

Query: 116 LKGLGRRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYGF 175
            + L         V +LLNPL        +AD I+    LM +Y       A  A     
Sbjct: 117 NQVLSTCSPLWISVIYLLNPLTFLPGIACSADMILNFTTLMTIYFASCGSYAIYACCMAL 176

Query: 176 AVHMKIYPVTYILPITLHLLPDRDNDKSLRQF 207
            V +    +    P  L L     + K  + F
Sbjct: 177 TVFINPNALLLFFPSYLILRKCNSSIKFRQIF 208
CPU time:   148.01 user secs.	    2.24 sys. secs	  150.25 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.318    0.180    0.586 

Gapped
Lambda     K      H
   0.270   0.0630    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 336425731
Number of Sequences: 887402
Number of extensions: 19353596
Number of successful extensions: 86749
Number of sequences better than 10.0: 278
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 261
Number of HSP's that attempted gapping in prelim test: 86383
Number of HSP's gapped (non-prelim): 495
length of query: 423
length of database: 277,845,442
effective HSP length: 48
effective length of query: 375
effective length of database: 235,250,146
effective search space: 88218804750
effective search space used: 88218804750
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 39 (20.4 bits)
S2: 75 (33.2 bits)