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GPI Anchor Biosynthesis Report: PIGL_SCHPO (PIG-L family, Schizosaccharomyces pombe)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (248 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 50 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

sp|Q9HDW9|PIGL_SCHPO Probable N-acetylglucosaminyl-phosphatidyli... 324 7e-88
sp|O35790|PIGL_RAT N-acetylglucosaminyl-phosphatidylinositol de-... 260 2e-68
gb|AAF55732.1| (AE003728) CG4433 gene product [Drosophila melano... 251 6e-66
ref|NP_004269.1| (NM_004278) phosphatidylinositol glycan, class ... 232 5e-60
ref|NP_014008.1| (NC_001145) N-acetylglucosaminylphosphatidylino... 216 4e-55
ref|NP_294173.1| (NC_001263) conserved hypothetical protein [Dei... 197 2e-49
ref|NP_127219.1| (NC_000868) hypothetical protein [Pyrococcus ab... 191 8e-48
emb|CAC18708.2| (AL451182) conserved hypothetical protein [Strep... 189 5e-47
ref|NP_142471.1| (NC_000961) hypothetical protein [Pyrococcus ho... 186 3e-46
ref|NP_565647.1| (NM_128293) similar to PIG-L [Arabidopsis thali... 185 7e-46
ref|NP_244186.1| (NC_002570) BH3320~unknown conserved protein [B... 179 4e-44
gb|AAL80478.1| (AE010159) hypothetical protein [Pyrococcus furio... 176 4e-43
ref|NP_242548.1| (NC_002570) BH1682~unknown conserved protein [B... 174 1e-42
ref|NP_293807.1| (NC_001263) conserved hypothetical protein [Dei... 163 2e-39
sp|P71311|YAIS_ECOLI HYPOTHETICAL 20.5 KDA PROTEIN IN ADHC-TAUA ... 161 1e-38
ref|NP_390128.1| (NC_000964) alternate gene name: jojG~similar t... 160 2e-38
ref|NP_215598.1| (NC_000962) hypothetical protein Rv1082 [Mycoba... 159 4e-38
ref|NP_376770.1| (NC_003106) 221aa long conserved hypothetical p... 159 5e-38
ref|NP_389828.1| (NC_000964) Uncharacterized conserved protein [... 159 6e-38
ref|NP_344220.1| (NC_002754) Conserved hypothetical protein [Sul... 159 6e-38
gb|AAC01723.1| (AF040570) negative regulatorly protein [Amycolat... 158 6e-38
ref|NP_371091.1| (NC_002758) conserved hypothetical protein [Sta... 158 1e-37
ref|NP_302547.1| (NC_002677) conserved hypothetical protein [Myc... 151 2e-35
ref|NP_302050.1| (NC_002677) conserved hypothetical protein [Myc... 144 1e-33
ref|NP_296086.1| (NC_001263) Uncharacterized conserved protein [... 142 7e-33
ref|NP_492873.1| (NM_060472) Y52B11C.1.p [Caenorhabditis elegans... 141 1e-32
emb|CAC16965.1| (AL450350) conserved hypothetical protein [Strep... 140 2e-32
emb|CAB66204.1| (AL136502) hypothetical protein SCF43.15c. [Stre... 137 3e-31
emb|CAC05756.1| (AL391751) hypothetical protein [Streptomyces co... 137 3e-31
emb|CAA77139.1| (Y18353) hypothetical protein [Thermus thermophi... 135 7e-31
gb|AAG12428.1| (AY005138) unknown [Chlorobium tepidum] 135 1e-30
gb|AAC14880.1| (AF060080) hypothetical protein [Chlorobium tepidum] 135 1e-30
ref|NP_437291.1| (NC_003078) conserved hypothetical protein, pos... 133 4e-30
pir||S44952 lmbE protein - Streptomyces lincolnensis >gi|2127551... 129 4e-29
ref|NP_356006.1| (NC_003063) AGR_L_453p [Agrobacterium tumefacie... 128 9e-29
ref|NP_535134.1| (NC_003305) conserved hypothetical protein [Agr... 128 9e-29
ref|NP_215686.1| (NC_000962) hypothetical protein Rv1170 [Mycoba... 128 1e-28
ref|NP_191372.1| (NM_115675) putative protein [Arabidopsis thali... 125 8e-28
gb|AAD41996.1|AC006233_13 (AC006233) hypothetical protein [Arabi... 122 5e-27
emb|CAB67717.1| (AJ271405) hypothetical protein [Streptomyces ro... 121 9e-27
ref|NP_334747.1| (NC_002755) hypothetical protein [Mycobacterium... 120 2e-26
emb|CAC36570.1| (AL590463) hypothetical protein [Streptomyces co... 120 2e-26
ref|NP_214837.1| (NC_000962) hypothetical protein Rv0323c [Mycob... 120 3e-26
ref|NP_105992.1| (NC_002678) hypothetical protein [Mesorhizobium... 114 2e-24
ref|NP_561406.1| (NC_003366) conserved hypothetical protein [Clo... 111 1e-23
ref|NP_294927.1| (NC_001263) LmbE-related protein [Deinococcus r... 107 2e-22
ref|NP_414898.1| (NC_000913) orf, hypothetical protein [Escheric... 107 3e-22
emb|CAC04222.1| (AL391515) conserved hypothetical protein [Strep... 101 8e-21
ref|NP_285456.1| (NC_001264) hypothetical protein [Deinococcus r... 98 1e-19
pir||D69906 hypothetical protein yojG - Bacillus subtilis >gi|26... 96 4e-19
Alignments
>sp|Q9HDW9|PIGL_SCHPO Probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
 emb|CAC21467.1| (AL512549) putative N-acetylglucosaminyl phosphatidylinositol
           deacetylase [Schizosaccharomyces pombe]
          Length = 248

 Score =  324 bits (824), Expect = 7e-88
 Identities = 248/248 (100%), Positives = 248/248 (100%)

Query: 1   MIWFWSTLLVTAIAVLSTANESSSGQEKLAVESILFVFAHPDDESMFFGPTIDYLGNQHS 60
           MIWFWSTLLVTAIAVLSTANESSSGQEKLAVESILFVFAHPDDESMFFGPTIDYLGNQHS
Sbjct: 1   MIWFWSTLLVTAIAVLSTANESSSGQEKLAVESILFVFAHPDDESMFFGPTIDYLGNQHS 60

Query: 61  TRVHVLCLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVA 120
           TRVHVLCLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVA
Sbjct: 61  TRVHVLCLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVA 120

Query: 121 KHISQIIERYNIKTLITFDNKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKY 180
           KHISQIIERYNIKTLITFDNKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKY
Sbjct: 121 KHISQIIERYNIKTLITFDNKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKY 180

Query: 181 ISYLDTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVRGHKSQMVWFRYGWIYLSKYMS 240
           ISYLDTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVRGHKSQMVWFRYGWIYLSKYMS
Sbjct: 181 ISYLDTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVRGHKSQMVWFRYGWIYLSKYMS 240

Query: 241 NNVLKRAT 248
           NNVLKRAT
Sbjct: 241 NNVLKRAT 248
>sp|O35790|PIGL_RAT N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
           (Phosphatidylinositol-glycan biosynthesis, class L
           protein) (PIG-L)
 dbj|BAA20869.1| (D88364) PIG-L [Rattus norvegicus]
          Length = 252

 Score =  260 bits (659), Expect = 2e-68
 Identities = 84/252 (33%), Positives = 124/252 (48%), Gaps = 21/252 (8%)

Query: 8   LLVTAIAVLS-------TANESSSGQE----KLAVESILFVFAHPDDESMFFGPTIDYLG 56
           LL  A+AVL+        + E     E      A    L V AHPDDE+MFF PTI  L 
Sbjct: 6   LLCVAVAVLTWGFLRVWNSAERMRSPEQAGLPGAGSRALVVIAHPDDEAMFFAPTILGLA 65

Query: 57  NQHSTRVHVLCLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDP 116
                +V +LC S+GN    G +R+KEL+ + +   I  + V ++   +  D  + +WD 
Sbjct: 66  RLK-QQVSLLCFSSGNYYNQGEIRKKELLQSCAVLGIPPSRVMIIDKREFPDDPEVQWDT 124

Query: 117 TDVAKHISQIIERYNIKTLITFDNKGISGHPNHIACYEGAMKI---VKATPQVQVFVLES 173
             VA  I Q I       ++TFD +G+SGH NHIA Y+    +    K      V  L+S
Sbjct: 125 EHVASTILQHIHANATDLVVTFDAEGVSGHSNHIALYKAVRALHSGGKLPEGCSVLTLQS 184

Query: 174 VNIFRKYISYLDTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVRGHKSQMVWFRYGWI 233
           VN+ RKY+  LD   TL+  Q      ++     K   + +   +  H+SQ++WFR+ + 
Sbjct: 185 VNVLRKYVFLLDLPWTLLSPQG-----VLFVLTSKEVAQAK-KAMSCHRSQLLWFRHLYT 238

Query: 234 YLSKYMSNNVLK 245
             S+YMS N L+
Sbjct: 239 VFSRYMSVNSLQ 250
>gb|AAF55732.1| (AE003728) CG4433 gene product [Drosophila melanogaster]
          Length = 390

 Score =  251 bits (637), Expect = 6e-66
 Identities = 83/253 (32%), Positives = 122/253 (47%), Gaps = 35/253 (13%)

Query: 22  SSSGQEKLAVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGN--------- 72
           S    +   +E +L + AHPDDE MFFGP I  L  +   +V++LCLSNG          
Sbjct: 130 SVRLPKTACMERVLLITAHPDDECMFFGPLIYSLTQRQGCQVYILCLSNGETTSSDIIPK 189

Query: 73  ------------ADGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVA 120
                        +    VR +EL  + SK  I ++N+ +++   L D     W P  VA
Sbjct: 190 PPIDLEALNESNFEHKAKVRRQELWRSCSKLGIPESNIVLMNATNLPDDPYVDWRPDAVA 249

Query: 121 KHISQIIERYNIKTLITFDNKGISGHPNHIACYEGAMKIVKAT-------PQVQVFVLES 173
             I   IE  +I+ + TFD  G+S HPNH A Y  A  +  A           + + L+S
Sbjct: 250 SLILHTIESLDIQAIFTFDRDGVSSHPNHCAVYYAAASLCLANLLPKGEEAYCKFYTLDS 309

Query: 174 VNIFRKYISYLDTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVRGHKSQMVWFRYGWI 233
           +N+ RKY+S LD + T   S      T     + K    +R AM+  H+SQM WFR+ +I
Sbjct: 310 INVVRKYLSILDLLCTCFMS------THWCILNWKEAAIVRSAMME-HQSQMRWFRWLYI 362

Query: 234 YLSKYMSNNVLKR 246
           Y S+YM  N +++
Sbjct: 363 YFSRYMFINSMRQ 375
>ref|NP_004269.1| (NM_004278) phosphatidylinositol glycan, class L [Homo sapiens]
 sp|Q9Y2B2|PIGL_HUMAN N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
           (Phosphatidylinositol-glycan biosynthesis, class L
           protein) (PIG-L)
 dbj|BAA74775.1| (AB017165) PIG-L [Homo sapiens]
          Length = 252

 Score =  232 bits (587), Expect = 5e-60
 Identities = 84/251 (33%), Positives = 125/251 (49%), Gaps = 21/251 (8%)

Query: 8   LLVTAIAVLS-------TANESSSGQEKLA----VESILFVFAHPDDESMFFGPTIDYLG 56
           LL  A+AVL+        ++E    +E+          L V AHPDDE+MFF PT+  L 
Sbjct: 6   LLCVALAVLAWGFLWVWDSSERMKSREQGGRLGAESRTLLVIAHPDDEAMFFAPTVLGLA 65

Query: 57  NQHSTRVHVLCLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDP 116
                 V++LC S GN    G  R+KEL+ +     I  ++V ++ +    D    +WD 
Sbjct: 66  RLRHW-VYLLCFSAGNYYNQGETRKKELLQSCDVLGIPLSSVMIIDNRDFPDDPGMQWDT 124

Query: 117 TDVAKHISQIIERYNIKTLITFDNKGISGHPNHIACYEGAMKI---VKATPQVQVFVLES 173
             VA+ + Q IE   I  ++TFD  G+SGH NHIA Y     +    K      V  L+S
Sbjct: 125 EHVARVLLQHIEVNGINLVVTFDAGGVSGHSNHIALYAAVRALHSEGKLPKGCSVLTLQS 184

Query: 174 VNIFRKYISYLDTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVRGHKSQMVWFRYGWI 233
           VN+ RKYIS LD   +L+ +Q      ++   + K   + +   +  H+SQ++WFR  +I
Sbjct: 185 VNVLRKYISLLDLPLSLLHTQ-----DVLFVLNSKEVAQAK-KAMSCHRSQLLWFRRLYI 238

Query: 234 YLSKYMSNNVL 244
             S+YM  N L
Sbjct: 239 IFSRYMRINSL 249
>ref|NP_014008.1| (NC_001145) N-acetylglucosaminylphosphatidylinositol
           de-N-acetylase; Gpi12p [Saccharomyces cerevisiae]
 sp|P23797|GP12_YEAST N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
 pir||S54588 probable membrane protein YMR281w - yeast (Saccharomyces
           cerevisiae)
 emb|CAA89779.1| (Z49704) unknown [Saccharomyces cerevisiae]
 dbj|BAA74776.1| (AB017166) GPI12 [Saccharomyces cerevisiae]
          Length = 304

 Score =  216 bits (545), Expect = 4e-55
 Identities = 82/266 (30%), Positives = 118/266 (43%), Gaps = 36/266 (13%)

Query: 15  VLSTANESSSG--QEKLAVESILFVFAHPDDESMFFGPTIDYLGNQHSTRV--HVLCLSN 70
           ++S  N S       K     I  V AHPDDE MFF P I  L +     V  +++CLS 
Sbjct: 36  IVSRNNASLQHIFPHKYGDYEINLVIAHPDDEVMFFSPIISQLNSYFPRTVPFNIICLSK 95

Query: 71  GNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIE-- 128
           GNA+GLG  R +EL  +A+    ++  V  V     QDGM   WD   +   +SQ I+  
Sbjct: 96  GNAEGLGETRVRELNESAALLLHNERAVS-VQVMDFQDGMDEIWDIDSITSSLSQKIDIK 154

Query: 129 RYNI-KTLITFDNKGISGHPNHIACYEGAMKIV-----------KATPQVQVFVLESV-- 174
            +N+ + ++TFD+ G+S H NH +CY    K+V           +  P V    L S   
Sbjct: 155 NHNLNQIIVTFDSYGVSNHINHKSCYAAVKKLVDDYAQPKTKRNEQPPHVTALYLRSYKN 214

Query: 175 NIFRKYISYL---------------DTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVR 219
           NI  KY S++                 I  L  + A   D + +             M+ 
Sbjct: 215 NIVLKYNSFIWEILKILYDLISPFRRIIQALPPNTAAEKDKLSLMNTHAQYVLAFATMLN 274

Query: 220 GHKSQMVWFRYGWIYLSKYMSNNVLK 245
            H+SQ+VWFRYGW   S+++  N   
Sbjct: 275 AHESQVVWFRYGWWIFSRFVFVNEFD 300
>ref|NP_294173.1| (NC_001263) conserved hypothetical protein [Deinococcus
           radiodurans]
 pir||F75517 conserved hypothetical protein - Deinococcus radiodurans  (strain
           R1)
 gb|AAF10027.1|AE001904_3 (AE001904) conserved hypothetical protein [Deinococcus radiodurans]
          Length = 281

 Score =  197 bits (497), Expect = 2e-49
 Identities = 58/233 (24%), Positives = 91/233 (38%), Gaps = 37/233 (15%)

Query: 22  SSSGQEKLAVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNA-------- 73
           SSS     A  ++L +FAHPDDE+   G T+ +   Q   RV + C + G A        
Sbjct: 3   SSSTPAPRA--TLLVIFAHPDDEAFSVGGTLTHYARQ-GVRVVLACATRGEAGKITVPGM 59

Query: 74  --DGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQ-------AKWDPTDVAKHIS 124
             D LG+ RE+EL  A    +I+        D    +  +          +P D    + 
Sbjct: 60  TVDDLGAQREQELREACRALEIEPPVFLDYHDSGRYERTRHDDPTALMNVNPLDAEVKLR 119

Query: 125 QIIERYNIKTLITFDNKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKY---- 180
            +IE    + ++TFD  G  GH +H+  +   +    +T       L S    R Y    
Sbjct: 120 ALIEDVQPQVIVTFDPHGAYGHVDHLQMHRATVAAFFSTGH-----LPSGGPQRLYYTAM 174

Query: 181 -------ISYLDTIPTLVQSQAGRND-TIIIHADRKSTQRIRDAMVRGHKSQM 225
                  IS L    +L     G +D T+ +  D  +    + A +  H +QM
Sbjct: 175 THQAAAQISRLGHDQSLDPLVYGVSDSTLAVTMDVGAYAENKKAALAAHGTQM 227
>ref|NP_127219.1| (NC_000868) hypothetical protein [Pyrococcus abyssi]
 pir||C75001 hypothetical protein PAB1341 - Pyrococcus abyssi (strain Orsay)
 emb|CAB50449.1| (AJ248288) hypothetical protein [Pyrococcus abyssi]
          Length = 267

 Score =  191 bits (483), Expect = 8e-48
 Identities = 40/215 (18%), Positives = 72/215 (32%), Gaps = 29/215 (13%)

Query: 29  LAVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNG---------NADGLGSV 79
             VE +L +  HPDD  +  G TI  L  +    V   C+++G             L ++
Sbjct: 29  KDVEKVLCIEPHPDDCVIGMGGTIKKLT-ERGIEVIYACMTDGYMGTLDSSLTGHELATI 87

Query: 80  REKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFD 139
           R +E   ++    + K       D +L    + + D       + +II +     +   D
Sbjct: 88  RRREEEESSKLLGVKKIYWLNYRDTELPYSREVRKD-------LVRIIRKEKPDGVFLPD 140

Query: 140 NKGIS-GHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRN 198
                  HP+H      A+  V  +P      L + +     I       + +       
Sbjct: 141 PWLPYEAHPDHRNTGFLALDAVAFSP------LPNFSNVDVEIGLGPHQVSFIALYYTNK 194

Query: 199 DTIIIHADRKSTQRIRDAMVRGHKSQM---VWFRY 230
               +  D      ++   +R HKSQ    VW  +
Sbjct: 195 PNYFV--DITDVMELKLKAIRTHKSQFPDDVWEVW 227
>emb|CAC18708.2| (AL451182) conserved hypothetical protein [Streptomyces coelicolor]
          Length = 293

 Score =  189 bits (476), Expect = 5e-47
 Identities = 47/250 (18%), Positives = 81/250 (31%), Gaps = 54/250 (21%)

Query: 33  SILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGN---------------ADGLG 77
            ++ V AHPDDES     T+    ++    V V+  + G                 + + 
Sbjct: 6   RLMAVHAHPDDESSKGAATMAKYVSE-GVDVLVVTCTGGERGSILNPKLQGDAYIEENIH 64

Query: 78  SVREKELVVAASKYQIDKTNVHVVSD--------PQLQDGMQAKWDPTDVAKHISQIIER 129
            VR KE+  A     + +  +  V          P L +G  A  D    A  + + I  
Sbjct: 65  EVRRKEMDEAREILGVGQEWLGFVDSGLPEGDPLPPLPEGCFALEDVDKAAGELVRKIRS 124

Query: 130 YNIKTLITFDNKGISGHPNHIACYEGAMKIVKATPQ---------------VQVFVLESV 174
           +  + + T+D  G   HP+HI  ++  M   +                   ++V+  +  
Sbjct: 125 FRPQVITTYDENGGYPHPDHIMTHKITMVAFEGAADTEKYPESEYGTAYQPLKVYYNQGF 184

Query: 175 NIFRKYI-----------SYLDTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVRGHKS 223
           N  R              S  +           +  T+  H        IRD  +  H +
Sbjct: 185 NRPRTEALHHALLDRGLESPYEDWLKRWSEFERKERTLTTHVPCADFFEIRDKALIAHAT 244

Query: 224 QMV----WFR 229
           Q+     WFR
Sbjct: 245 QIDPEGGWFR 254
>ref|NP_142471.1| (NC_000961) hypothetical protein [Pyrococcus horikoshii]
 pir||F71162 hypothetical protein PH0499 - Pyrococcus horikoshii
 dbj|BAA29587.1| (AP000002) 272aa long hypothetical protein [Pyrococcus horikoshii]
          Length = 272

 Score =  186 bits (470), Expect = 3e-46
 Identities = 37/217 (17%), Positives = 74/217 (34%), Gaps = 32/217 (14%)

Query: 29  LAVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNG---------NADGLGSV 79
              + +L +  HPDD  +  G TI  L +     V  +C+++G         +   L ++
Sbjct: 33  KDAKKVLCIEPHPDDCVIGMGGTIKKL-SDMGVEVIYVCMTDGYMGTTDESLSGHELAAI 91

Query: 80  REKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFD 139
           R KE   +A    + K       D +L    + + D       +++I+ +     +   D
Sbjct: 92  RRKEEEESARLLGVKKIYWLNYRDTELPYSREVRKD-------LTKILRKEQPDGVFAPD 144

Query: 140 NKGIS-GHPNHIACYEGAMK--IVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAG 196
                  HP+H      A++       P      L+         S++    T   +   
Sbjct: 145 PWLPYESHPDHRRTGFLAIESVAFSQLPNFSNTDLDIGLNPYNSGSFIALYYTHKPN--- 201

Query: 197 RNDTIIIHADRKSTQRIRDAMVRGHKSQM---VWFRY 230
                    D      ++   +R H+SQ    +W ++
Sbjct: 202 ------YIVDITDLMELKLKAIRVHRSQFPDDIWEKW 232
>ref|NP_565647.1| (NM_128293) similar to PIG-L [Arabidopsis thaliana]
          Length = 223

 Score =  185 bits (466), Expect = 7e-46
 Identities = 60/207 (28%), Positives = 98/207 (46%), Gaps = 29/207 (14%)

Query: 43  DESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVS 102
           D+  FF PTI+Y  +  +  +H+LC S GNADG+GS+R++EL  A +  ++         
Sbjct: 34  DDGKFFSPTINYFTST-ACNLHILCFSTGNADGMGSIRDQELHRACAVLKVIP------- 85

Query: 103 DPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPNHIACYEGAMKIVKA 162
                      +D   +  + S      N + +ITFDN G+ GH NH   +      + +
Sbjct: 86  -----------FDKEGICDNDSCHC---NEEHIITFDNYGVWGHCNHRDVHPPIDCKIDS 131

Query: 163 TPQVQVFVL---ESVNIFRKYISYLDTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVR 219
             ++  F+     S+NIFRKY   +D   +++ ++   +  III+   K   +     + 
Sbjct: 132 AKRIHGFLYVHQVSLNIFRKYCGPVDIWLSILSAKRHPSKVIIIN---KQPWKSF-KAMA 187

Query: 220 GHKSQMVWFRYGWIYLSKYMSNNVLKR 246
            H SQ VWFR  ++  S Y   N L R
Sbjct: 188 QHLSQWVWFRKLFVLFSSYTYVNTLDR 214
>ref|NP_244186.1| (NC_002570) BH3320~unknown conserved protein [Bacillus halodurans]
 dbj|BAB07039.1| (AP001518) BH3320~unknown conserved protein [Bacillus halodurans]
          Length = 227

 Score =  179 bits (452), Expect = 4e-44
 Identities = 47/208 (22%), Positives = 73/208 (34%), Gaps = 33/208 (15%)

Query: 32  ESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNA------------DGLGSV 79
             +L +F HPDDE+     TI  L  +    V   CL+ G              + L  +
Sbjct: 5   RHVLVIFPHPDDEAFGVSGTIA-LFRKQGVPVTYACLTLGEMGRNLGNPPFATRESLPDI 63

Query: 80  REKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFD 139
           R+KEL+ +A    I+   +        +D      D T +   +S ++   N   +ITF 
Sbjct: 64  RKKELIKSAEAMGIEDLRMLGY-----RDKTIEFEDETKLTDMVSDLMAELNPSLIITFY 118

Query: 140 NKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRND 199
             G S HP+H A     ++ V+   +     L  V     +   L     L         
Sbjct: 119 P-GYSVHPDHEATGRAVVRAVRRLEKSMRPKLYGVAFSNGHQEELGDPDILF-------- 169

Query: 200 TIIIHADRKSTQRIRDAMVRGHKSQMVW 227
                 D       + A +R H SQ  W
Sbjct: 170 ------DISPVAEQKKAAIRAHISQTAW 191
>gb|AAL80478.1| (AE010159) hypothetical protein [Pyrococcus furiosus DSM 3638]
          Length = 267

 Score =  176 bits (443), Expect = 4e-43
 Identities = 35/215 (16%), Positives = 74/215 (34%), Gaps = 33/215 (15%)

Query: 31  VESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNG---------NADGLGSVRE 81
            + ++ +  HPDD ++  G TI  L ++    V  +C+++G         +   L  +R 
Sbjct: 31  AKKVICIEPHPDDCAIGMGGTIKKLSDE-GVEVIYICMTDGYMGTTDEKLSGHELALIRR 89

Query: 82  KELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNK 141
           +E   +A    + K       D +L    + + D       + +II +     +   D  
Sbjct: 90  REEEESAKLLGVRKIYWLNYRDTELPYSREVRKD-------LVKIIRKEKPDGVFAPDPW 142

Query: 142 GIS-GHPNHIACYEGAMK--IVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRN 198
                HP+H      A++       P      ++        +S++    T   +     
Sbjct: 143 LPYESHPDHRRTGFLAIESVAFSQLPNFSNIDIDIGLKPH-SVSFIALYYTHKPN----- 196

Query: 199 DTIIIHADRKSTQRIRDAMVRGHKSQM---VWFRY 230
                  D      ++   +R H+SQ    +W  +
Sbjct: 197 ----YIVDITDLMELKLKAIRAHRSQFTDDIWETW 227
>ref|NP_242548.1| (NC_002570) BH1682~unknown conserved protein [Bacillus halodurans]
 dbj|BAB05401.1| (AP001512) BH1682~unknown conserved protein [Bacillus halodurans]
          Length = 231

 Score =  174 bits (439), Expect = 1e-42
 Identities = 39/194 (20%), Positives = 73/194 (37%), Gaps = 21/194 (10%)

Query: 34  ILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLGSV--REKELVVAASKY 91
           IL   AHPDD  +  G T+ +   Q   RV +  L+       G+V  R+KE   A+   
Sbjct: 6   ILAFGAHPDDVEIGMGATLYHY-RQKGHRVGICNLTKAELSSNGTVEQRQKEAADASRIL 64

Query: 92  QIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPNHIA 151
            ID+     + D  L++  + +       ++I  +I          F    +  HP+H  
Sbjct: 65  GIDERIQLDLPDRGLRNPSEQQV------RNIVSVIRHCQP--TFVFVPYPVDRHPDHGH 116

Query: 152 CYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRNDTIIIHADRKSTQ 211
           C E   + V    +++ +  E      + + Y           +     +++  D     
Sbjct: 117 CAELVKEAVFNA-RIRNYKAEGGAHHVQDLFY-------YMINSFERPDLLV--DVSHCY 166

Query: 212 RIRDAMVRGHKSQM 225
            ++ A +  +KSQ 
Sbjct: 167 EVKQAALNAYKSQF 180
>ref|NP_293807.1| (NC_001263) conserved hypothetical protein [Deinococcus
           radiodurans]
 pir||G75562 conserved hypothetical protein - Deinococcus radiodurans  (strain
           R1)
 gb|AAF09674.1|AE001871_6 (AE001871) conserved hypothetical protein [Deinococcus radiodurans]
          Length = 237

 Score =  163 bits (411), Expect = 2e-39
 Identities = 36/206 (17%), Positives = 65/206 (31%), Gaps = 28/206 (13%)

Query: 33  SILFVFAHPDDESMFFGPTID-YLGNQHSTRVHVLCLSNGNA----------DGLGSVRE 81
            +L +  HPDDE      T+  YL    S    ++ L+ G A          + L  +R 
Sbjct: 14  KLLLIVPHPDDEVYGASGTLMEYLAAGESC--GLVTLTRGEAGRTLGLCDGPEELARMRA 71

Query: 82  KELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNK 141
            EL        +  T   +    Q  D     +   ++ +   + +ER   +T++TF   
Sbjct: 72  VELAACLEVIGLTTTPGSLHEQHQFPDKYLKDYPFEELVETAREAMERLRPETVLTFPPN 131

Query: 142 GISGHPNHIACYEGAMKIVKATPQVQV---FVLESVNIFRKYISYLDTIPTLVQSQAGRN 198
           G +GHP+H+  +          P       +   S                         
Sbjct: 132 GSNGHPDHMTTHRAVKAAWDRLPAGSRPVLWYYASETPPENE-ELRAAWLPP-------- 182

Query: 199 DTIIIHADRKSTQRIRDAMVRGHKSQ 224
               +  D  +    +   +  H+SQ
Sbjct: 183 ---TVKRDVSALVTRKLQAIACHRSQ 205
>sp|P71311|YAIS_ECOLI HYPOTHETICAL 20.5 KDA PROTEIN IN ADHC-TAUA INTERGENIC REGION
 gb|AAB18087.1| (U73857) hypothetical protein [Escherichia coli]
          Length = 185

 Score =  161 bits (405), Expect = 1e-38
 Identities = 38/211 (18%), Positives = 77/211 (36%), Gaps = 32/211 (15%)

Query: 16  LSTANESSSGQEKLAVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADG 75
           +  +   SS  ++     IL + AHPDD  +  G ++  L  Q    +  + ++ GN+  
Sbjct: 4   VLDSALLSSANKRKG---ILAIGAHPDDIELGCGASLARLA-QKGIYIAAVVMTTGNSGT 59

Query: 76  LGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIER---YNI 132
            G +   E    A K       +H+       D  +A     D+   +  II+     ++
Sbjct: 60  DGIIDRHEESRNALKILGCHQTIHL----NFAD-TRAHLQLNDMISALEDIIKNQIPSDV 114

Query: 133 KTLITFDNKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQ 192
           + +  +       H +H+A Y+ +M   +  PQ+  +   S            T  +   
Sbjct: 115 EIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPS------------TWLS-FM 161

Query: 193 SQAGRNDTIIIHADRKSTQRIRDAMVRGHKS 223
            Q   +         +    ++ A ++ HKS
Sbjct: 162 PQVFESVK-------EEYFTVKLAALKKHKS 185
>ref|NP_390128.1| (NC_000964) alternate gene name: jojG~similar to hypothetical
           proteins [Bacillus subtilis]
 sp|P42981|YPJG_BACSU Hypothetical 24.8 kDa protein in DAPB-PAPS intergenic region
 pir||F69937 conserved hypothetical protein ypjG - Bacillus subtilis
 gb|AAA92876.1| (L38424) unknown [Bacillus subtilis]
 gb|AAB38444.1| (L47709) putative [Bacillus subtilis]
 emb|CAB14163.1| (Z99115) alternate gene name: jojG~similar to hypothetical proteins
           [Bacillus subtilis]
          Length = 224

 Score =  160 bits (402), Expect = 2e-38
 Identities = 36/199 (18%), Positives = 56/199 (28%), Gaps = 28/199 (14%)

Query: 34  ILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLGSV--REKELVVAASKY 91
           +L   AH DD  +  G TI     Q   +V +  L+       G+V  R++E   AA   
Sbjct: 6   VLAFGAHSDDVEIGMGGTIAKFVKQE-KKVMICDLTEAELSSNGTVSLRKEEAAEAARIL 64

Query: 92  QIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPNHIA 151
             DK     + D  L              + I  +I     K +  F       HP+H  
Sbjct: 65  GADKRIQLTLPDRGL-------IMSDQAIRSIVTVIRICRPKAV--FMPYKKDRHPDHGN 115

Query: 152 CYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRNDTII----IHADR 207
                 + + +                KY          V                  D 
Sbjct: 116 AAALVEEAIFSAG------------IHKYKDEKSLPAHKVSKVYYYMINGFHQPDFVIDI 163

Query: 208 KSTQRIRDAMVRGHKSQMV 226
             T   +   +  +KSQ +
Sbjct: 164 SDTIEAKKQSLNAYKSQFI 182
>ref|NP_215598.1| (NC_000962) hypothetical protein Rv1082 [Mycobacterium tuberculosis
           H37Rv]
 ref|NP_335555.1| (NC_002755) lmbE protein [Mycobacterium tuberculosis CDC1551]
 pir||H70894 hypothetical protein Rv1082 - Mycobacterium tuberculosis  (strain
           H37RV)
 emb|CAA17198.1| (AL021897) hypothetical protein Rv1082 [Mycobacterium tuberculosis
           H37Rv]
 gb|AAK45369.1| (AE006992) lmbE protein [Mycobacterium tuberculosis CDC1551]
          Length = 288

 Score =  159 bits (400), Expect = 4e-38
 Identities = 39/241 (16%), Positives = 74/241 (30%), Gaps = 48/241 (19%)

Query: 33  SILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADG--------------LGS 78
            ++ V AHPDDES     T+    ++   RV V+ L+ G                  +  
Sbjct: 5   RLMAVHAHPDDESSKGAATLARYADE-GHRVLVVTLTGGERGEILNPAMDLPDVHGRIAE 63

Query: 79  VREKELVVAASKYQIDKTNVHVVSD--------PQLQDGMQAKWDPTDVAKHISQIIERY 130
           +R  E+  AA    ++ T +  V          P L D   A+       + + +++  +
Sbjct: 64  IRRDEMTKAAEILGVEHTWLGFVDSGLPKGDLPPPLPDDCFARVPLEVSTEALVRVVREF 123

Query: 131 NIKTLITFDNKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYI--------- 181
               + T+D  G   HP+HI C++ ++   +A      F           +         
Sbjct: 124 RPHVMTTYDENGGYPHPDHIRCHQVSVAAYEAAGDFCRFPDAGEPWTVSKLYYVHGFLRE 183

Query: 182 ---------------SYLDTIPTLV-QSQAGRNDTIIIHADRKSTQRIRDAMVRGHKSQM 225
                             +                +    +       RD  +R H +Q+
Sbjct: 184 RMQMLQDEFARHGQRGPFEQWLAYWDPDHDFLTSRVTTRVECSKYFSQRDDALRAHATQI 243

Query: 226 V 226
            
Sbjct: 244 D 244
>ref|NP_376770.1| (NC_003106) 221aa long conserved hypothetical protein [Sulfolobus
           tokodaii]
 dbj|BAB65879.1| (AP000984) 221aa long conserved hypothetical protein [Sulfolobus
           tokodaii]
          Length = 221

 Score =  159 bits (399), Expect = 5e-38
 Identities = 43/215 (20%), Positives = 79/215 (36%), Gaps = 39/215 (18%)

Query: 33  SILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADG---------LGSVREKE 83
            ILF+  HPDDE    G T+  L    S  ++++ +++G+A           L  +R KE
Sbjct: 2   RILFISPHPDDECDNAGGTLAKLA--KSHEIYIVYMTDGSAGSPNPEERGEKLAEIRRKE 59

Query: 84  LVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGI 143
            +       I K N   ++ P  +     +     VAK + +I        +I      +
Sbjct: 60  ALEGLKVLGIKKDNAFFLNYPDTKLRFHIREASERVAKILREI----KPNIII--YPSLL 113

Query: 144 SGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRNDTIII 203
            GH +H +              +    +  V I    +SYL+    +           ++
Sbjct: 114 DGHNDHWSGGY-----------ITRIAIRKVGITVNELSYLN-WLPIPSKSVFDAIKYLL 161

Query: 204 H-------ADRKSTQRIRDAMVRGHKSQMVWFRYG 231
                    D +  +RI+   ++ H+SQ   F+Y 
Sbjct: 162 IPFHRKIKVDIREYKRIKLEAMKKHESQ---FKYL 193
>ref|NP_389828.1| (NC_000964) Uncharacterized conserved protein [Bacillus subtilis]
          Length = 221

 Score =  159 bits (399), Expect = 6e-38
 Identities = 44/210 (20%), Positives = 79/210 (36%), Gaps = 43/210 (20%)

Query: 32  ESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNA------------DGLGSV 79
           E +L +  HPDDES      I  L  +    V   C + G              + L  +
Sbjct: 3   EHVLVILPHPDDESYGVAGLIA-LNRKKDIPVTYACATLGEMGRNMGDPFFANRETLPLL 61

Query: 80  REKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFD 139
           R++EL+ A  +  I+   +       L+D      D   +A  + +II+      ++TF 
Sbjct: 62  RKQELINACKEMDINDLRML-----GLRDKTLEFEDDEYLADIMEEIIDDVKPSLIVTFY 116

Query: 140 N-KGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRN 198
              G+  HP+H AC E  ++ +                +RK     D   T+  +     
Sbjct: 117 PGHGV--HPDHDACGEAVIRAL----------------YRKKKE--DRPRTICMAITRNR 156

Query: 199 DTII----IHADRKSTQRIRDAMVRGHKSQ 224
           + ++    +  D K    I+   +R H++Q
Sbjct: 157 EEVLGEADVVLDIKEVADIKMNALRAHRTQ 186
>ref|NP_344220.1| (NC_002754) Conserved hypothetical protein [Sulfolobus
           solfataricus]
 gb|AAK43010.1| (AE006882) Conserved hypothetical protein [Sulfolobus solfataricus]
          Length = 193

 Score =  159 bits (399), Expect = 6e-38
 Identities = 33/210 (15%), Positives = 66/210 (30%), Gaps = 47/210 (22%)

Query: 26  QEKLAVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNAD----------G 75
           Q+   +  +L V  HPDDE++  G TI     +   ++ V+ +++G              
Sbjct: 2   QKSSCMRRVLIVAPHPDDETLCCGGTIQIF-KEKGYKISVIIVTDGRYGSPDDKLKGSSE 60

Query: 76  LGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTL 135
           L  +R +E + A     ID+       D         K    D    +++ +   +    
Sbjct: 61  LIEIRRQEALRATKILGIDEVKFLNFEDS--------KVSEEDAENALAEFLREND---- 108

Query: 136 ITFDNKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQA 195
           + F       HP+H    +   K+         F L   N    +               
Sbjct: 109 VVFSPIPFDNHPDHANIGKAVEKLYPNA----YFYLIWGNTQVNWRE------------- 151

Query: 196 GRNDTIIIHADRKSTQRIRDAMVRGHKSQM 225
                  +  D +  +  +   +  + SQ+
Sbjct: 152 -------VKFDIRKYKESKLRAINQYISQI 174
>gb|AAC01723.1| (AF040570) negative regulatorly protein [Amycolatopsis
           mediterranei]
          Length = 255

 Score =  158 bits (398), Expect = 6e-38
 Identities = 37/216 (17%), Positives = 73/216 (33%), Gaps = 22/216 (10%)

Query: 31  VESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNG-----------NADGLGSV 79
           + +++   AHP+D++   G  +    ++   RV ++  + G             + LG  
Sbjct: 1   MGTLVSFHAHPNDDTTTCGGVLRK-AHEDGHRVVLVLATRGELGYNPDGLLAEGETLGDR 59

Query: 80  REKELVVAASKYQIDKTNVHVVSDPQL---QD--GMQAKWDPTDVAKHISQIIERYNIKT 134
           R  E   AA    +D+      +D  +    D  G     D  + A+ ++ I+       
Sbjct: 60  RAVEARAAADVLGVDRLEFLGYTDSGMTAAADGAGTFQTADVEEAARKLAAILREERADV 119

Query: 135 LITFDNKGISGHPNHIACYEGAMKIVKATPQVQVF--VLESVNI---FRKYISYLDTIPT 189
           L  +D KG  G P+HI  +    +  +     +VF   +   +I    R           
Sbjct: 120 LTVYDEKGTYGDPDHIQVHRVGTRAAELAGTAKVFQSTINREHIKANQRVLAEQAGVDLP 179

Query: 190 LVQSQAGRNDTIIIHADRKSTQRIRDAMVRGHKSQM 225
                      +    D  +    +   +  H SQ+
Sbjct: 180 AGPDFGTPEAELTCRVDVSAYTEYKRKALLAHASQI 215
>ref|NP_371091.1| (NC_002758) conserved hypothetical protein [Staphylococcus aureus
           subsp. aureus Mu50]
 ref|NP_373778.1| (NC_002745) conserved hypothetical protein [Staphylococcus aureus
           subsp. aureus N315]
 dbj|BAB41756.1| (AP003131) conserved hypothetical protein [Staphylococcus aureus
           subsp. aureus N315]
 dbj|BAB56729.1| (AP003359) conserved hypothetical protein [Staphylococcus aureus
           subsp. aureus Mu50]
          Length = 221

 Score =  158 bits (396), Expect = 1e-37
 Identities = 44/209 (21%), Positives = 70/209 (33%), Gaps = 33/209 (15%)

Query: 32  ESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNA------------DGLGSV 79
             +L +F HPDDE+     T+     Q    V   CL+ G              + L S+
Sbjct: 5   RHVLVIFPHPDDETFSSAGTLASYI-QKGIPVTYACLTLGQMGRNLGNPPFATRESLPSI 63

Query: 80  REKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFD 139
           RE+EL  A     I       +    L+D          +   I  +I+  N   +I+F 
Sbjct: 64  RERELEEACKVIGIT-----DLRKMGLRDKTVEFEPYEHIDGMIKSLIDDTNPSLIISFY 118

Query: 140 NKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRND 199
             G + HP+H A  +  ++ V+  P+ +   L  V         L               
Sbjct: 119 P-GYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATEALGEPD----------- 166

Query: 200 TIIIHADRKSTQRIRDAMVRGHKSQMVWF 228
              I  D    + ++      H SQ   F
Sbjct: 167 ---IQNDITDFKELKIKAFEAHASQTGPF 192
>ref|NP_302547.1| (NC_002677) conserved hypothetical protein [Mycobacterium leprae]
 gb|AAA63037.1| (U15183) lmbE gene product [Mycobacterium leprae]
 emb|CAC31907.1| (AL583925) conserved hypothetical protein [Mycobacterium leprae]
          Length = 290

 Score =  151 bits (378), Expect = 2e-35
 Identities = 46/241 (19%), Positives = 83/241 (34%), Gaps = 48/241 (19%)

Query: 33  SILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGN--------------ADGLGS 78
            ++ V AHPDDES     T+    ++   RV V+ L+ G                  +  
Sbjct: 5   RLMAVHAHPDDESSKGAATLARYADE-GHRVLVVTLTGGERGEILNPAMDLPDVHGHIAE 63

Query: 79  VREKELVVAASKYQIDKTNVHVVSD--------PQLQDGMQAKWDPTDVAKHISQIIERY 130
           +R  E+  AA    ++ T +  +          P L D   A        + + +++ ++
Sbjct: 64  IRRDEMAKAAEILGVEHTWLGFIDSGLPKGDPPPPLPDDCFALVPLEVCTEALVRVVRKF 123

Query: 131 NIKTLITFDNKGISGHPNHIACYEGAMKIVKATPQVQVF----------VLESVNIFRKY 180
               L T+D  G   HP+HI C++ ++   +A    + F           L   + F + 
Sbjct: 124 RPHVLTTYDENGGYPHPDHIRCHQVSVDAYEAACDYRRFPDAGKPWTVSKLYYNHGFLRA 183

Query: 181 ISYLDTIPTLVQSQAGRNDT---------------IIIHADRKSTQRIRDAMVRGHKSQM 225
              L         QAG  D                +    +  +    RD  +R H +Q+
Sbjct: 184 RMQLLHDEFAKHGQAGPFDKWLAQSNPAHDPFESRVTTRVECSAYFSQRDDALRAHATQI 243

Query: 226 V 226
            
Sbjct: 244 D 244
>ref|NP_302050.1| (NC_002677) conserved hypothetical protein [Mycobacterium leprae]
 emb|CAC30445.1| (AL583922) conserved hypothetical protein [Mycobacterium leprae]
          Length = 308

 Score =  144 bits (362), Expect = 1e-33
 Identities = 46/249 (18%), Positives = 74/249 (29%), Gaps = 56/249 (22%)

Query: 33  SILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGN----------------ADGL 76
            +LFV AHPDDES+  G TI +  ++   +V V+  + G                 AD L
Sbjct: 6   RLLFVHAHPDDESLSNGATIAHYTSR-GAQVQVVTCTLGEEGEVIGDRWAELTVDHADQL 64

Query: 77  GSVREKELVVAASKYQIDKTNVHVVSDPQLQDGM----------QAKWDPTDVAKHISQI 126
           G  R  EL  A     +        +      GM              D  +    +  I
Sbjct: 65  GGYRIFELTEALRALGVSAPIYLGGAGRWRDSGMRGTAPRRRQRFIDADENEAVGALVAI 124

Query: 127 IERYNIKTLITFDNKGISGHPNHIACYEGAMKIVKA-----------------TPQVQVF 169
           I       ++T+D  G  GHP+H+  +      V +                   +V  F
Sbjct: 125 IRELRPHVVVTYDPHGGYGHPDHVHTHFITAAAVASSGVAAGLEVGADEYPGKPWKVPKF 184

Query: 170 VLESVNIFRKYISY-------LDTIPTLVQ----SQAGRNDTI-IIHADRKSTQRIRDAM 217
                 +              L    T+           +  I  +          + A 
Sbjct: 185 YWSVFALSAFEAGMNALQGKDLRPEWTIPPREEFYFGYSDKDIDAVVEATSDVWAAKTAA 244

Query: 218 VRGHKSQMV 226
           +  H +Q+V
Sbjct: 245 LTAHATQVV 253
>ref|NP_296086.1| (NC_001263) Uncharacterized conserved protein [Deinococcus
           radiodurans]
          Length = 239

 Score =  142 bits (355), Expect = 7e-33
 Identities = 38/196 (19%), Positives = 64/196 (32%), Gaps = 28/196 (14%)

Query: 35  LFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLG--SVREKELVVAASKYQ 92
           L +  HPDD  +  G T+  L  Q    V +L L+ G     G  + R+ E V AA    
Sbjct: 18  LCLAPHPDDAEIGAGGTLIRLA-QAGRAVGILELTRGEKGTQGTPAERQAECVAAARLMD 76

Query: 93  IDKTNVHVVSDPQLQD-GMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPNHIA 151
           +       + D +L D    A          ++  +     + L+         HP+H  
Sbjct: 77  LSWRGQLGLPDGELADTPPFAH--------ALAAALRTVRPRVLVV--PHWHDRHPDHFG 126

Query: 152 CYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTI--PTLVQSQAGRNDTIIIHADRKS 209
            Y    + +                 +K     D      ++  Q   + +  +  D  S
Sbjct: 127 TYHLTKRAIHLAA------------LKKADLGGDPWRVQRVLLYQGNSDISANVLVDIGS 174

Query: 210 TQRIRDAMVRGHKSQM 225
                +A +R H SQ 
Sbjct: 175 VMTEWEAAIRAHTSQF 190
>ref|NP_492873.1| (NM_060472) Y52B11C.1.p [Caenorhabditis elegans]
 pir||T27111 hypothetical protein Y52B11C.1 - Caenorhabditis elegans
 emb|CAA19544.1| (AL023846) Y52B11C.1 [Caenorhabditis elegans]
          Length = 151

 Score =  141 bits (353), Expect = 1e-32
 Identities = 59/143 (41%), Positives = 81/143 (56%), Gaps = 4/143 (2%)

Query: 8   LLVTAIAVLSTANESSSGQEKLAVE-SILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVL 66
           L+VT + VL       S    L  +  IL + AHPDDE+MFF PTI  L  Q   RV VL
Sbjct: 7   LIVTLLLVLLLIFAVRSHPIPLLSQSRILLLIAHPDDETMFFSPTIRALL-QAGHRVFVL 65

Query: 67  CLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQI 126
           C+SNGN DGLG +R +EL  AASK  I  ++V  +   +  DG    W+   + + + + 
Sbjct: 66  CISNGNFDGLGKIRARELSRAASKLGISASDVICLDYDEFADG--DTWNRNALCQIVMRH 123

Query: 127 IERYNIKTLITFDNKGISGHPNH 149
           +E     T+I+FD+ G+SGH NH
Sbjct: 124 VEVLAADTVISFDSHGVSGHHNH 146
>emb|CAC16965.1| (AL450350) conserved hypothetical protein [Streptomyces coelicolor]
          Length = 277

 Score =  140 bits (351), Expect = 2e-32
 Identities = 39/228 (17%), Positives = 71/228 (31%), Gaps = 39/228 (17%)

Query: 34  ILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSN-----------GNADGLGS---- 78
           ++ V AHPDDE+   G  +     +    V V C              G+     +    
Sbjct: 8   LMAVHAHPDDEATSTGGVLARYAAEGIRTVLVTCTDGGCGDGPGGVKPGDPGHDPAAVAL 67

Query: 79  VREKELVVAASKYQIDKTNVHVVSDPQL-----QDGMQAKW--DPTDVAKHISQIIERYN 131
           +R +EL  +    +I        +D  +      D   + W     + A  +++++  Y 
Sbjct: 68  MRRRELEESRDILKISDLETLDYADSGMMGWPSNDAPGSFWRTPVEEGAARLAELMRHYR 127

Query: 132 IKTLITFDNKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLV 191
              ++T+D  G  GHP+HI  +   M  ++ T              R  +     I    
Sbjct: 128 PDVVVTYDENGFYGHPDHIQAHRITMAALEMTTLTPKVY--WTTAPRSMMQRFGEIMREF 185

Query: 192 QS---------------QAGRNDTIIIHADRKSTQRIRDAMVRGHKSQ 224
                                ++ I    D  S    +   +  H SQ
Sbjct: 186 HPDMPEPDPAEAAAMAEIGLPDEEITTWVDTTSFSGQKFDALAAHASQ 233
>emb|CAB66204.1| (AL136502) hypothetical protein SCF43.15c. [Streptomyces coelicolor
           A3(2)]
          Length = 247

 Score =  137 bits (342), Expect = 3e-31
 Identities = 27/161 (16%), Positives = 51/161 (30%), Gaps = 20/161 (12%)

Query: 16  LSTANESSSGQEKLAVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNAD- 74
           ++    +            L V AHPDD        I    ++    V  +  + G A  
Sbjct: 1   MTEPTITQLEPMPGDWRRALAVVAHPDDLEYGCAAAIAAWTDE-GREVAYVLATRGEAGI 59

Query: 75  ------GLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIE 128
                     +RE+E   +A+   + +       D  ++ G         + + I+  I 
Sbjct: 60  DTLAPAECAPLREREQRASAAVVGVSEVEFLDHRDGVVEYGT-------ALRRDIAAAIR 112

Query: 129 RYNIKTLITF---DNKGI--SGHPNHIACYEGAMKIVKATP 164
           R+  + +IT    D  G      P+H+A     +       
Sbjct: 113 RHRPELVITMNHRDTWGGVAWNTPDHVAVGRATLDAAADAG 153
>emb|CAC05756.1| (AL391751) hypothetical protein [Streptomyces coelicolor A3(2)]
          Length = 295

 Score =  137 bits (342), Expect = 3e-31
 Identities = 38/160 (23%), Positives = 57/160 (34%), Gaps = 25/160 (15%)

Query: 29  LAVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADG------------- 75
           L    +L V AHPDDES+  G T+     + +    V C + G                 
Sbjct: 4   LPGRRLLLVHAHPDDESINNGVTMARYAAEGAHVTLVTC-TLGERGEVIPPALAHLSGAA 62

Query: 76  LGSVREKELVVAASKYQIDKTNVHV----VSDPQL-----QDGMQAKWD--PTDVAKHIS 124
           LG  R  EL  A     +D   +       +D  +      D     W       A  + 
Sbjct: 63  LGGHRRGELADAMRALGVDDFRLLGGPGRYADSGMLGLSDNDDPGCLWQADVDAAAALLV 122

Query: 125 QIIERYNIKTLITFDNKGISGHPNHIACYEGAMKIVKATP 164
            +I     + L+T+D  G  GHP+HI  +  AM+  +   
Sbjct: 123 DVIREVRPQVLVTYDPNGGYGHPDHIQAHRIAMRAAELAA 162
>emb|CAA77139.1| (Y18353) hypothetical protein [Thermus thermophilus]
          Length = 227

 Score =  135 bits (338), Expect = 7e-31
 Identities = 37/197 (18%), Positives = 63/197 (31%), Gaps = 22/197 (11%)

Query: 31  VESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLG--SVREKELVVAA 88
           +  +L V  HPDD  +  G T+            +L L+ G     G    REKE+  A+
Sbjct: 1   MLDLLVVAPHPDDGELGCGGTLAR-AKAEGLSTGILDLTRGEMGSKGTPEEREKEVAEAS 59

Query: 89  SKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPN 148
               +D        +    DG  A      +   ++Q + R   + +  F       HP+
Sbjct: 60  RILGLDFR-----GNLGFPDGGLADVPEQRL--KLAQALRRLRPRVV--FAPLEADRHPD 110

Query: 149 HIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRNDTIIIHADRK 208
           H A    A+  V      +   LE      + + +         S               
Sbjct: 111 HTAASRLAVAAVHLAGLRKAP-LEGEPFRVERLFFYPGNHPFAPS---------FLVKIS 160

Query: 209 STQRIRDAMVRGHKSQM 225
           +     +A V  ++SQ 
Sbjct: 161 AFIDQWEAAVLAYRSQF 177
>gb|AAG12428.1| (AY005138) unknown [Chlorobium tepidum]
          Length = 250

 Score =  135 bits (337), Expect = 1e-30
 Identities = 37/193 (19%), Positives = 64/193 (32%), Gaps = 17/193 (8%)

Query: 35  LFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLG--SVREKELVVAASKYQ 92
           L   AHPDD  +  G T+  + ++    V V  L+ G    LG    R +E  +A  +  
Sbjct: 13  LAFGAHPDDVELACGATLLKIMDE-GKPVAVCDLTAGEMGTLGTAETRRQEAALATERMG 71

Query: 93  IDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPNHIAC 152
                   + D +L       +    + K I +II +Y   T+  F N     HP+H+  
Sbjct: 72  YVAREQLDLGDSEL------FYTKESLHKII-RIIRKYRPDTV--FCNPPDERHPDHMKA 122

Query: 153 YEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRNDTIIIHADRKSTQR 212
                +        ++   +       +         L+     +     I  D  ST  
Sbjct: 123 SRLIYEACYYAGLRKIETFDGGLPQAAH-----RPRHLLYYIQFKQLEPQIVVDVSSTFE 177

Query: 213 IRDAMVRGHKSQM 225
              A +    +Q 
Sbjct: 178 RSRAGIEAFGTQF 190
>gb|AAC14880.1| (AF060080) hypothetical protein [Chlorobium tepidum]
          Length = 240

 Score =  135 bits (337), Expect = 1e-30
 Identities = 37/193 (19%), Positives = 64/193 (32%), Gaps = 17/193 (8%)

Query: 35  LFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLG--SVREKELVVAASKYQ 92
           L   AHPDD  +  G T+  + ++    V V  L+ G    LG    R +E  +A  +  
Sbjct: 13  LAFGAHPDDVELACGATLLKIMDE-GKPVAVCDLTAGEMGTLGTAETRRQEAALATERMG 71

Query: 93  IDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPNHIAC 152
                   + D +L       +    + K I +II +Y   T+  F N     HP+H+  
Sbjct: 72  YVAREQLDLGDSEL------FYTKESLHKII-RIIRKYRPDTV--FCNPPDERHPDHMKA 122

Query: 153 YEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRNDTIIIHADRKSTQR 212
                +        ++   +       +         L+     +     I  D  ST  
Sbjct: 123 SRLIYEACYYAGLRKIETFDGGLPQAAH-----RPRHLLYYIQFKQLEPQIVVDVSSTFE 177

Query: 213 IRDAMVRGHKSQM 225
              A +    +Q 
Sbjct: 178 RSRAGIEAFGTQF 190
>ref|NP_437291.1| (NC_003078) conserved hypothetical protein, possibly
           membrane-associated [Sinorhizobium meliloti]
 emb|CAC49151.1| (AL603644) conserved hypothetical protein, possibly
           membrane-associated [Sinorhizobium meliloti]
          Length = 228

 Score =  133 bits (332), Expect = 4e-30
 Identities = 35/204 (17%), Positives = 63/204 (30%), Gaps = 32/204 (15%)

Query: 33  SILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNG---NADGLGSVREK-ELVVAA 88
             L V  HPDDE +  G TI  L  +    V V  ++ G     D   + R + E   A 
Sbjct: 10  RTLVVAPHPDDEVLGAGGTIARLAAE-GEEVFVAVVTEGKPPAFDPEATARIQAEARQAH 68

Query: 89  SKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPN 148
               + +T    +   QL +   A  +       + +++ R + +T++         H +
Sbjct: 69  RALGVTETIWLRLPAAQLAETAHATVN-----AALLELVHRLSPQTVLL--PFVGDMHMD 121

Query: 149 HIACYEGAMKIV-----KATPQVQVFVLESV-NIFRKYISYLDTIPTLVQSQAGRNDTII 202
           H   +  A+        +    V  +   S  N    Y+S                    
Sbjct: 122 HQLTFTSALVACRPHQAEFPKLVLAYETLSETNWNAPYLSPA------FVPNVF------ 169

Query: 203 IHADRKSTQRIRDAMVRGHKSQMV 226
              D       +   +    SQ+ 
Sbjct: 170 --VDISEHLEAKLKAMELFASQVR 191
>pir||S44952 lmbE protein - Streptomyces lincolnensis
 pir||S69814 lmbE protein - Streptomyces lincolnensis
 emb|CAA55751.1| (X79146) lmbE [Streptomyces lincolnensis]
          Length = 270

 Score =  129 bits (323), Expect = 4e-29
 Identities = 45/234 (19%), Positives = 74/234 (31%), Gaps = 39/234 (16%)

Query: 32  ESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLG-------------S 78
           + +L V AHPDDE+   G T+ +   Q    V V C   G  + L              +
Sbjct: 3   QCLLTVHAHPDDEASRGGATVAHYTAQGVRAVLVTCTDGGAGEVLNPAVTDDFTPERFVA 62

Query: 79  VREKELVVAASKYQIDKTNVHVVSDPQLQDGM------QAKWDPTDVAKHISQIIERYNI 132
           VR  EL  +A        +     D  + DG         +    + A  ++++I     
Sbjct: 63  VRSAELDASARNLGYSAVHRLGYRDSGM-DGTAGGAEAFVRAPLDEAATRLARVIADERP 121

Query: 133 KTLITF-DNKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYI---------- 181
             +I +  N     HP+HI   E   + V            + +  R             
Sbjct: 122 DVVIGYGTNHTRDPHPDHIRANEVLTRRVDLLDHTPAVYHIAFSRRRHRALHQACVDSGV 181

Query: 182 -SYLDTIPTLVQSQAGRNDTIIIHADR--KSTQRIRDAMVRGHKSQMV----WF 228
            S  +   +     A  ++ I    D         R   +R H +Q+     WF
Sbjct: 182 PSPYEGGLSA-PPGAFDDEWITTLVDVTKGDAVERRLDALRSHVTQVPPASGWF 234
>ref|NP_356006.1| (NC_003063) AGR_L_453p [Agrobacterium tumefaciens] [Agrobacterium
           tumefaciens str. C58 (Cereon)]
 gb|AAK88791.1| (AE008221) AGR_L_453p [Agrobacterium tumefaciens str. C58 (Cereon)]
          Length = 815

 Score =  128 bits (320), Expect = 9e-29
 Identities = 34/177 (19%), Positives = 60/177 (33%), Gaps = 28/177 (15%)

Query: 16  LSTANESSSGQEKLAVESILFV---FAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGN 72
           ++        ++  A++S + V    AHPDDE       + Y   +   R  + C + G 
Sbjct: 27  MADPWLIRLHRKLSALKSTVTVMHTGAHPDDEQ---NGLLAYFRTELGMRTIIACSTRGE 83

Query: 73  ADG----------LGSVREKELVVAASKYQIDKTNVH-----VVSDPQLQ---DGMQAKW 114
                        LG +R +EL  AA     D + +      ++ D       D    +W
Sbjct: 84  GGQNALGPERLGALGVIRSRELEEAARVIDADISWLGHGPADIIHDFGFSKDGDQTFGRW 143

Query: 115 DPTDVAKHISQIIERYNIKTLI-TF-DNKGISGHPNHIACYEGAMKIVKATPQVQVF 169
               + + + +   +     +I TF D  G  GH  H A    A   +        +
Sbjct: 144 GQNRIVERLVRAYRKERPDIVIPTFLDVPGQHGH--HRAMTRAAKSAITLAADPSAY 198
>ref|NP_535134.1| (NC_003305) conserved hypothetical protein [Agrobacterium
           tumefaciens str. C58 (U. Washington)]
 gb|AAL45450.1| (AE009394) conserved hypothetical protein [Agrobacterium
           tumefaciens str. C58 (U. Washington)]
          Length = 800

 Score =  128 bits (320), Expect = 9e-29
 Identities = 34/177 (19%), Positives = 60/177 (33%), Gaps = 28/177 (15%)

Query: 16  LSTANESSSGQEKLAVESILFV---FAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGN 72
           ++        ++  A++S + V    AHPDDE       + Y   +   R  + C + G 
Sbjct: 12  MADPWLIRLHRKLSALKSTVTVMHTGAHPDDEQ---NGLLAYFRTELGMRTIIACSTRGE 68

Query: 73  ADG----------LGSVREKELVVAASKYQIDKTNVH-----VVSDPQLQ---DGMQAKW 114
                        LG +R +EL  AA     D + +      ++ D       D    +W
Sbjct: 69  GGQNALGPERLGALGVIRSRELEEAARVIDADISWLGHGPADIIHDFGFSKDGDQTFGRW 128

Query: 115 DPTDVAKHISQIIERYNIKTLI-TF-DNKGISGHPNHIACYEGAMKIVKATPQVQVF 169
               + + + +   +     +I TF D  G  GH  H A    A   +        +
Sbjct: 129 GQNRIVERLVRAYRKERPDIVIPTFLDVPGQHGH--HRAMTRAAKSAITLAADPSAY 183
>ref|NP_215686.1| (NC_000962) hypothetical protein Rv1170 [Mycobacterium tuberculosis
           H37Rv]
 ref|NP_335650.1| (NC_002755) lmbE-related protein [Mycobacterium tuberculosis
           CDC1551]
 pir||B70875 hypothetical protein Rv1170 - Mycobacterium tuberculosis  (strain
           H37RV)
 emb|CAA15847.1| (AL010186) hypothetical protein Rv1170 [Mycobacterium tuberculosis
           H37Rv]
 gb|AAK45464.1| (AE006998) lmbE-related protein [Mycobacterium tuberculosis
           CDC1551]
          Length = 303

 Score =  128 bits (320), Expect = 1e-28
 Identities = 37/142 (26%), Positives = 52/142 (36%), Gaps = 27/142 (19%)

Query: 33  SILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGN----------------ADGL 76
            +LFV AHPDDES+  G TI +  ++   +VHV+  + G                 AD L
Sbjct: 6   RLLFVHAHPDDESLSNGATIAHYTSR-GAQVHVVTCTLGEEGEVIGDRWAQLTADHADQL 64

Query: 77  GSVREKELVVAASKYQIDKTNVHVVSDPQLQDGM----------QAKWDPTDVAKHISQI 126
           G  R  EL  A     +        +      GM              DP      +  I
Sbjct: 65  GGYRIGELTAALRALGVSAPIYLGGAGRWRDSGMAGTDQRSQRRFVDADPRQTVGALVAI 124

Query: 127 IERYNIKTLITFDNKGISGHPN 148
           I       ++T+D  G  GHP+
Sbjct: 125 IRELRPHVVVTYDPNGGYGHPD 146
>ref|NP_191372.1| (NM_115675) putative protein [Arabidopsis thaliana]
 pir||T45973 hypothetical protein F9D24.40 - Arabidopsis thaliana
 emb|CAB68151.1| (AL137081) putative protein [Arabidopsis thaliana]
          Length = 124

 Score =  125 bits (312), Expect = 8e-28
 Identities = 44/111 (39%), Positives = 70/111 (62%), Gaps = 1/111 (0%)

Query: 47  FFGPTIDYLGNQHSTRVHVLCLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQL 106
           FF PTI+YL + ++  +H+LCLS GNADG+GS+R  EL  A +  ++    + +++ P L
Sbjct: 7   FFSPTINYLAS-NACNLHMLCLSTGNADGMGSIRNNELHRACAVLKVPLQQLKILNHPNL 65

Query: 107 QDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPNHIACYEGAM 157
           QDG    W    + + I + + +++I T+ITFDN G+SGH NH   + G +
Sbjct: 66  QDGFGQLWSHDLLTEIIEEEVTKHDIHTIITFDNYGVSGHCNHRDVHRGVL 116
>gb|AAD41996.1|AC006233_13 (AC006233) hypothetical protein [Arabidopsis thaliana]
          Length = 185

 Score =  122 bits (305), Expect = 5e-27
 Identities = 43/154 (27%), Positives = 71/154 (45%), Gaps = 35/154 (22%)

Query: 47  FFGPTIDYLGNQHSTRVHVLCLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQL 106
           FF PTI+Y  +  +  +H+LC S GNADG+GS+R++EL  A +  ++             
Sbjct: 51  FFSPTINYFTST-ACNLHILCFSTGNADGMGSIRDQELHRACAVLKVIP----------- 98

Query: 107 QDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPNHIACYEGAMKIVKATPQV 166
                  +D   +  + S      N + +ITFDN G+ GH NH   + G +         
Sbjct: 99  -------FDKEGICDNDSCHC---NEEHIITFDNYGVWGHCNHRDVHRGVL--------- 139

Query: 167 QVFVLESVNIFRKYISYLDTIPTLVQSQAGRNDT 200
                 S+NIFRKY   +D   +++ ++   +  
Sbjct: 140 ----YVSLNIFRKYCGPVDIWLSILSAKRHPSKV 169
>emb|CAB67717.1| (AJ271405) hypothetical protein [Streptomyces rochei]
          Length = 234

 Score =  121 bits (303), Expect = 9e-27
 Identities = 22/204 (10%), Positives = 52/204 (24%), Gaps = 26/204 (12%)

Query: 30  AVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLGSVREKELVV--- 86
            V  +L V  HPDD+ +  G ++     + +    V+ +    +    +V E E      
Sbjct: 3   GVTDVLVVAPHPDDDVIGCGGSMAKHVREGARVTVVVVIGRERSALDDAVTEAEFAAETE 62

Query: 87  -AASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISG 145
            AA    + +      S        +   D       + +++     + +  +       
Sbjct: 63  NAAKILGVHRCVRFDESSRDFAPSRRIHLD-------LVRVLREVRPQVV--YLPHDNDD 113

Query: 146 HPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQA---GRNDTII 202
              H   +    + +           +S                 +              
Sbjct: 114 DVEHRMVHRLTTEALWMA--------QSEFFQ--EAGECPMPAPRLVLGYEVWSPMARYQ 163

Query: 203 IHADRKSTQRIRDAMVRGHKSQMV 226
              D       +   +R + SQ+ 
Sbjct: 164 YAEDIGEHIHTKVEAMRAYVSQLR 187
>ref|NP_334747.1| (NC_002755) hypothetical protein [Mycobacterium tuberculosis
           CDC1551]
 gb|AAK44561.1| (AE006940) hypothetical protein [Mycobacterium tuberculosis
           CDC1551]
          Length = 223

 Score =  120 bits (300), Expect = 2e-26
 Identities = 38/129 (29%), Positives = 57/129 (43%), Gaps = 15/129 (11%)

Query: 30  AVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADG-------LGSVREK 82
               +L VFAHPDDES   G  +     Q  TR+  LC ++G A         LG VR +
Sbjct: 9   CAHEVLAVFAHPDDESFGLGAVLGDFTAQ-GTRLRGLCFTHGEASTLGRTDRNLGEVRRE 67

Query: 83  ELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKG 142
           EL  AA    +D   +    D  L     A+    ++ + +   +       L+ FD+ G
Sbjct: 68  ELAAAAQVLGVDHVQLLAYPDNGL-----AQIPLNELTQRVVDALA--GADLLLVFDDNG 120

Query: 143 ISGHPNHIA 151
           ++GHP+H  
Sbjct: 121 VTGHPDHRR 129
>emb|CAC36570.1| (AL590463) hypothetical protein [Streptomyces coelicolor]
 emb|CAC36831.1| (AL590464) hypothetical protein [Streptomyces coelicolor]
          Length = 218

 Score =  120 bits (299), Expect = 2e-26
 Identities = 33/206 (16%), Positives = 57/206 (27%), Gaps = 34/206 (16%)

Query: 31  VESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLG-SVREKELVVAAS 89
           + S+L V AHPDD  +  G  I +    +   V V CL+ G     G  VR +E + A  
Sbjct: 1   MTSVLVVVAHPDDAEIAMGMRIHWYA-LNGATVRVHCLTTGTPAPDGTEVRRQECLSAGE 59

Query: 90  KYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPNH 149
              +D+     + D +  +          +   +  +        + T        H +H
Sbjct: 60  LLGVDQYTFSSIPDTRFVENRG------RINADLFDVFREARPDIIYTHYPD--DQHLDH 111

Query: 150 IACYEGAMKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRNDTIIIHADRKS 209
                                     + R+    L    +      G             
Sbjct: 112 SVTAREVT-----------------TVARREAPNLRHFRS--PYSVGFEPNEFF-FGTAE 151

Query: 210 TQRIRDAMVRGHKSQ----MVWFRYG 231
               +   ++   SQ    M  FR  
Sbjct: 152 LLEAKVRALKCFASQTQLDMDVFRQL 177
>ref|NP_214837.1| (NC_000962) hypothetical protein Rv0323c [Mycobacterium
           tuberculosis H37Rv]
 pir||D70526 hypothetical protein Rv0323c - Mycobacterium tuberculosis  (strain
           H37RV)
 emb|CAB09612.1| (Z96800) hypothetical protein Rv0323c [Mycobacterium tuberculosis
           H37Rv]
          Length = 223

 Score =  120 bits (299), Expect = 3e-26
 Identities = 38/129 (29%), Positives = 57/129 (43%), Gaps = 15/129 (11%)

Query: 30  AVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADG-------LGSVREK 82
               +L VFAHPDDES   G  +     Q  TR+  LC ++G A         LG VR +
Sbjct: 9   CAHEVLAVFAHPDDESFGLGAVLGDFTAQ-GTRLRGLCFTHGEASTLGRTDRNLGEVRRE 67

Query: 83  ELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKG 142
           EL  AA    +D   +    D  L     A+    ++ + +   +       L+ FD+ G
Sbjct: 68  ELAAAAQVLGVDHVQLLAYPDNGL-----AQIPLNELTQRVVDALA--GADLLLVFDDNG 120

Query: 143 ISGHPNHIA 151
           ++GHP+H  
Sbjct: 121 VTGHPDHRR 129
>ref|NP_105992.1| (NC_002678) hypothetical protein [Mesorhizobium loti]
 dbj|BAB51778.1| (AP003006) hypothetical protein [Mesorhizobium loti]
          Length = 229

 Score =  114 bits (283), Expect = 2e-24
 Identities = 36/199 (18%), Positives = 58/199 (29%), Gaps = 41/199 (20%)

Query: 33  SILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADG------LGSVREKELVV 86
            IL + AHPDD  +F   T+     Q    +     ++G   G      L  VR +E   
Sbjct: 2   KILALGAHPDDIEIFMFGTLAVYAAQ-GAELTFAVATDGAKGGKSDATVLARVRREEATA 60

Query: 87  AASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGH 146
           AA         +         DG         +   +  +I       +IT        H
Sbjct: 61  AAGLLGAAPRFL------DFPDG--ELVADAALIGALKTLIAGTGPDLVITHAPNDY--H 110

Query: 147 PNHIACYEGA-MKIVKATPQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRNDTIIIHA 205
            +H A  +   +    A P +                             G + T  +  
Sbjct: 111 ADHRALSDSVRIAASFAVPVLHADT---------------------MGGTGFSPTHYV-- 147

Query: 206 DRKSTQRIRDAMVRGHKSQ 224
           D  +   I+   +R H+SQ
Sbjct: 148 DISAHAEIKAKAIRMHQSQ 166
>ref|NP_561406.1| (NC_003366) conserved hypothetical protein [Clostridium
           perfringens]
 dbj|BAB80196.1| (AP003187) conserved hypothetical protein [Clostridium perfringens]
          Length = 601

 Score =  111 bits (277), Expect = 1e-23
 Identities = 35/119 (29%), Positives = 51/119 (42%), Gaps = 5/119 (4%)

Query: 32  ESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADGLGSVREKELVVAASKY 91
           + I+ +  H DDE    G TI  L + +   V V+  +NG+  GLG+ R KE V A    
Sbjct: 51  KEIMVIVPHQDDEINLAGATIKRLID-NGNNVKVVFATNGDFKGLGTKRIKEAVEAVRIL 109

Query: 92  QIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQII---ERYNIKTLITFDNKGISGHP 147
            ++  NV  +      +  +     +D  K IS  I   E Y     + F    ISG P
Sbjct: 110 GVNSENVIFLGYGDRWEETKEHIYNSDDNKIISSYIGKNETYGTDKYLDF-RSSISGEP 167
>ref|NP_294927.1| (NC_001263) LmbE-related protein [Deinococcus radiodurans]
 pir||B75424 LmbE-related protein - Deinococcus radiodurans (strain R1)
 gb|AAF10773.1|AE001969_2 (AE001969) LmbE-related protein [Deinococcus radiodurans]
          Length = 252

 Score =  107 bits (266), Expect = 2e-22
 Identities = 26/140 (18%), Positives = 51/140 (35%), Gaps = 21/140 (15%)

Query: 33  SILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNG---------NADGLGSVREKE 83
            I+ VFAHPDDE +    T+     +    V ++  + G           + +  +R + 
Sbjct: 2   RIMAVFAHPDDE-IGCIGTLAKHARR-GDEVLLVWTTLGELASQFGDTEHEEVRRIRREH 59

Query: 84  LVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGI 143
               A K    K +   + D ++  G          A  ++++   +    +IT+ +   
Sbjct: 60  GAWVADKIGA-KYHFFDMGDSRMTGGRDE-------ALQLARLYATFRPHAVITWSDD-- 109

Query: 144 SGHPNHIACYEGAMKIVKAT 163
             HP+H    + A   V   
Sbjct: 110 HPHPDHRMTAKIAFDAVTLA 129
>ref|NP_414898.1| (NC_000913) orf, hypothetical protein [Escherichia coli K12]
 pir||D64764 hypothetical protein b0364 - Escherichia coli
 gb|AAC73467.1| (AE000143) orf, hypothetical protein [Escherichia coli K12]
          Length = 136

 Score =  107 bits (265), Expect = 3e-22
 Identities = 26/160 (16%), Positives = 55/160 (34%), Gaps = 28/160 (17%)

Query: 67  CLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQI 126
            ++ GN+   G +   E    A K       +H+       D  +A     D+   +  I
Sbjct: 2   VMTTGNSGTDGIIDRHEESRNALKILGCHQTIHL----NFAD-TRAHLQLNDMISALEDI 56

Query: 127 IER---YNIKTLITFDNKGISGHPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISY 183
           I+     +++ +  +       H +H+A Y+ +M   +  PQ+  +   S          
Sbjct: 57  IKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPS---------- 106

Query: 184 LDTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVRGHKS 223
             T  +    Q   +         +    ++ A ++ HKS
Sbjct: 107 --TWLS-FMPQVFESVK-------EEYFTVKLAALKKHKS 136
>emb|CAC04222.1| (AL391515) conserved hypothetical protein [Streptomyces coelicolor]
          Length = 247

 Score =  101 bits (252), Expect = 8e-21
 Identities = 29/156 (18%), Positives = 47/156 (29%), Gaps = 10/156 (6%)

Query: 16  LSTANESSSGQEKLAVESILFVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNA-- 73
           ++ +  +            L V AHPDD        +          V  L  + G A  
Sbjct: 1   MTDSAPAPLRSMPDDWRRALAVVAHPDDLEYGCSAAVASWVA-DGKDVAYLLATRGEAGI 59

Query: 74  DGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIK 133
           D L   R   L  A  +       V  V     +DG         + + I+  + R+  +
Sbjct: 60  DTLDPGRAGPLREAEQRAAAAAVGVRAVEFLDHRDG--VIEYGASLRRDIAAAVRRHRPE 117

Query: 134 TLITF---DNK--GISGHPNHIACYEGAMKIVKATP 164
            +IT    D    G    P+H+A     +       
Sbjct: 118 LVITLNHRDTWAAGAWNTPDHVAVGRAVLDAAADAG 153
>ref|NP_285456.1| (NC_001264) hypothetical protein [Deinococcus radiodurans]
 pir||G75608 hypothetical protein - Deinococcus radiodurans (strain R1)
 gb|AAF12317.1|AE001862_143 (AE001862) hypothetical protein [Deinococcus radiodurans]
          Length = 232

 Score = 98.1 bits (242), Expect = 1e-19
 Identities = 33/205 (16%), Positives = 61/205 (29%), Gaps = 38/205 (18%)

Query: 36  FVFAHPDDESMFFGPTIDYLGNQHSTRVHVLCLSNGNADG----------LGSVREKELV 85
            V  HPDDE++  G  +     +    V  L L++G              L +VR  E  
Sbjct: 15  VVAPHPDDEALGCG-ALLAALAEAGREVWALLLTDGGFSHPASKAYPRPRLSAVRLAEWR 73

Query: 86  VAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISG 145
              S   +       +    L DG   +         + Q   +    T++         
Sbjct: 74  EGLSVLGVPPARTVAL---GLPDGALGEHLTAAARAQVRQAFAQARPGTVLL--PWERDP 128

Query: 146 HPNHIACYEGAMKIVKATPQVQVFVLESVNIFRKYISYL-----DTIPTLVQSQAGRNDT 200
           HP+H A              +   VL S  +  +Y  +L     D            ++ 
Sbjct: 129 HPDHRA-----------AWHLLRGVLPSDTLALEYAVWLPERGADADWP------RPDEV 171

Query: 201 IIIHADRKSTQRIRDAMVRGHKSQM 225
             +       +  +   +  H++Q+
Sbjct: 172 EELTFAVGDWRDAKARAIASHRTQL 196
>pir||D69906 hypothetical protein yojG - Bacillus subtilis
 emb|CAB13838.1| (Z99114) yojG [Bacillus subtilis]
 gb|AAC17855.1| (AF026147) YojG [Bacillus subtilis]
          Length = 142

 Score = 96.5 bits (238), Expect = 4e-19
 Identities = 25/125 (20%), Positives = 48/125 (38%), Gaps = 25/125 (20%)

Query: 105 QLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDN-KGISGHPNHIACYEGAMKIVKAT 163
            L+D      D   +A  + +II+      ++TF    G+  HP+H AC E  ++ +   
Sbjct: 3   GLRDKTLEFEDDEYLADIMEEIIDDVKPSLIVTFYPGHGV--HPDHDACGEAVIRAL--- 57

Query: 164 PQVQVFVLESVNIFRKYISYLDTIPTLVQSQAGRNDTII----IHADRKSTQRIRDAMVR 219
                        +RK     D   T+  +     + ++    +  D K    I+   +R
Sbjct: 58  -------------YRKKKE--DRPRTICMAITRNREEVLGEADVVLDIKEVADIKMNALR 102

Query: 220 GHKSQ 224
            H++Q
Sbjct: 103 AHRTQ 107
CPU time:    70.23 user secs.	    2.38 sys. secs	   72.61 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.315    0.172    0.507 

Gapped
Lambda     K      H
   0.270   0.0604    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 177889407
Number of Sequences: 887402
Number of extensions: 8577099
Number of successful extensions: 29481
Number of sequences better than 10.0: 198
Number of HSP's better than 10.0 without gapping: 66
Number of HSP's successfully gapped in prelim test: 132
Number of HSP's that attempted gapping in prelim test: 29179
Number of HSP's gapped (non-prelim): 213
length of query: 248
length of database: 277,845,442
effective HSP length: 56
effective length of query: 192
effective length of database: 228,150,930
effective search space: 43804978560
effective search space used: 43804978560
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.2 bits)
S2: 72 (32.1 bits)