Sequences with E-value BETTER than threshold (0.002)
emb|CAB64544.1| (AJ131718) legumain-like protease [Zea mays] 523 e-147
sp|P49043|VPE_CITSI VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) >... 519 e-146
gb|AAK15049.1|AF238384_1 (AF238384) asparaginyl endopeptidase [V... 518 e-146
emb|CAC18099.1| (AJ251453) putative legumain [Zea mays] 518 e-146
ref|NP_180165.1| (NM_128154) putative vacuolar processing enzyme... 518 e-146
dbj|BAA76745.1| (D89972) asparaginyl endopeptidase (VmPE-1A) [Vi... 516 e-145
emb|CAB64545.1| (AJ131719) legumain-like protease [Zea mays] 516 e-145
gb|AAF69014.1|AF260827_1 (AF260827) cysteine protease [Ipomoea b... 514 e-145
pir||S60049 vacuolar processing enzyme (EC 3.4.22.-) isozyme alp... 513 e-144
ref|XP_039644.1| (XM_039644) phosphatidylinositol glycan, class ... 512 e-144
dbj|BAA76744.1| (D89971) asparaginyl endopeptidase (VmPE-1) [Vig... 512 e-144
gb|AAD04883.1| (AF082347) C13 endopeptidase NP1 precursor [Zea m... 512 e-144
pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney ... 511 e-143
emb|CAC18100.1| (AJ251454) putative legumain [Zea mays] 509 e-143
emb|CAA68871.1| (Y07596) gpi8 [Homo sapiens] 507 e-142
sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE) >... 507 e-142
gb|AAF89679.1| (AF169973) asparaginyl endopeptidase [Sesamum ind... 504 e-141
ref|NP_195020.1| (NM_119448) gamma-VPE (vacuolar processing enzy... 502 e-141
sp|Q39119|VPEG_ARATH VACUOLAR PROCESSING ENZYME, GAMMA-ISOZYME P... 502 e-141
emb|CAC43295.1| (AJ309173) putative vacuolar processing enzyme [... 500 e-140
dbj|BAA84650.1| (AB025310) asparaginyl endopeptidase [Oryza sati... 499 e-140
sp|P49045|VPE_SOYBN VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) >... 498 e-140
pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney ... 498 e-140
sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptida... 496 e-139
sp|P49044|VPE_VICSA VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) (... 494 e-139
gb|AAF89646.1|AF169019_1 (AF169019) seed maturation protein PM40... 491 e-138
dbj|BAB29018.1| (AK013853) data source:SPTR, source key:O14822, ... 491 e-138
ref|NP_176458.1| (NM_104948) beta-VPE [Arabidopsis thaliana] >gi... 488 e-137
pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme bet... 479 e-134
pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring... 479 e-134
emb|CAB16318.1| (Z99174) cysteine proteinase precursor [Vicia na... 478 e-134
ref|NP_563825.1| (NM_100749) putative GPI-anchor transamidase (U... 471 e-132
gb|AAF45703.1| (AE003422) EG:133E12.3 gene product [Drosophila m... 454 e-127
pir||T13411 hypothetical protein 133E12.3 - fruit fly (Drosophil... 454 e-126
emb|CAB42655.1| (AJ238882) putative preprolegumain [Vicia narbon... 452 e-126
gb|AAF73260.1|AF154349_1 (AF154349) legumain [Rattus norvegicus] 445 e-124
emb|CAB51545.1| (AJ243876) vacuolar processing enzyme [Lycopersi... 444 e-123
ref|NP_071562.1| (NM_022226) protease, cysteine, 1 (legumain) [R... 443 e-123
ref|NP_035305.1| (NM_011175) protease, cysteine, 1; preproleguma... 442 e-123
ref|NP_188656.1| (NM_112912) vacuolar processing enzyme/asparagi... 441 e-122
pir||T00731 hypothetical protein F22O13.26 - Arabidopsis thaliana 439 e-122
dbj|BAA09530.1| (D55696) cysteine protease [Homo sapiens] 437 e-121
ref|NP_010618.1| (NC_001136) Protein involved in the attachment ... 436 e-121
ref|XP_007399.4| (XM_007399) legumain [Homo sapiens] 435 e-121
emb|CAB42651.1| (AJ238881) putative preprolegumain [Nicotiana ta... 435 e-121
gb|AAH03061.1|AAH03061 (BC003061) protease, cysteine, 1 (legumai... 435 e-121
ref|NP_005597.1| (NM_005606) legumain; protease, cysteine, 1 (le... 435 e-121
dbj|BAB69947.1| (AB060129) legumain [Bos taurus] 431 e-120
gb|AAF99765.1|AC003981_15 (AC003981) F22O13.24 [Arabidopsis thal... 427 e-118
pir||T40853 probable cysteine proteinase (EC 3.4.22.-) [similari... 426 e-118
Alignments
>emb|CAB64544.1| (AJ131718) legumain-like protease [Zea mays]
Length = 486
Score = 523 bits (1335), Expect = e-147
Identities = 73/353 (20%), Positives = 145/353 (40%), Gaps = 31/353 (8%)
Query: 5 LSFILPIFLIFSTVLSSDSPNDSVN------INNSDPSRHSNNWAVLVSTSRFWFNYRHM 58
L+ +L + L + + + +D WAVL++ S ++NYRH
Sbjct: 8 LALLLSVCLCSAWARPRLETAIRLPSQRAAAADETDDGAVGTRWAVLIAGSSGYYNYRHQ 67
Query: 59 ANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYR 118
A+ Y+ +K+ G+ D II+ + DDIA +P N PG + N+ + D+Y + DY
Sbjct: 68 ADICHAYQIMKKGGLKDENIIVFMYDDIAHSPENPRPGVIINHP-QGGDVYA-GVPKDYT 125
Query: 119 GYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAH 177
G +V V+NF + L +K K + S + +IF++ + HGG L +
Sbjct: 126 GRDVNVDNFFAVLLGNKTALRGGSGKVVDSGPDDHIFVFYSDHGGPGVLGMPTYPYLYGD 185
Query: 178 DLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKIH--SPNILAVGSSEIEESSYSHHS-- 233
DL D + + Y + F ++ C++ +++E + N+ A +S EESS+ +
Sbjct: 186 DLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPG 245
Query: 234 -----DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVH----SHPGI 282
+ + D Y++ ++E D + E++ + + S+
Sbjct: 246 EFPSPPPEYDTCLGDL---YSVAWMEDSDFHNLRTESLKQQYKLVKDRTAVHDTFSYGSH 302
Query: 283 RTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
++ L + G + +E+ + ++ NQ +
Sbjct: 303 VMQYGALELNVQHLFSYIG----TDPANDGNTFIEDNSLPSFSKAVNQRDADL 351
>sp|P49043|VPE_CITSI VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||S51117 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - sweet
orange
emb|CAA87720.1| (Z47793) cystein proteinase (by similarity) [Citrus sinensis]
prf||2208463A vascular processing protease [Citrus sinensis]
Length = 494
Score = 519 bits (1324), Expect = e-146
Identities = 79/339 (23%), Positives = 143/339 (41%), Gaps = 23/339 (6%)
Query: 11 IFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVKR 70
I + S N D WAVL++ S ++NYRH A+ Y+ +++
Sbjct: 30 ILKLPSEAYRFFHNGGGGAKVNDDDDSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRK 89
Query: 71 LGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMR- 129
G+ D II+ + DDIA N N PG + N+ D+Y + + DY G +VTVE F
Sbjct: 90 GGLKDENIIVFMYDDIAFNEENPRPGVIINHP-HGDDVY-KGVPKDYTGEDVTVEKFFAV 147
Query: 130 LLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQ 189
+L +K K + S N +IFI+ + HGG L + I A +L D + +
Sbjct: 148 VLGNKTALTGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSRYIYADELIDVLKKKHAS 207
Query: 190 KRYNEIFFMIDTCQANTMYEK--IHSPNILAVGSSEIEESSYSHHS-------DMDIGVA 240
Y + F ++ C++ +++E + NI A +S EESS+ + +
Sbjct: 208 GNYKSLVFYLEACESGSIFEGLLLEGLNIYATTASNAEESSWGTYCPGEIPGPPPEYSTC 267
Query: 241 VIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENV--HSHPGIRTDLFKRNVSEVLL 296
+ D Y++ ++E D + ET+ + + +S+ +S+ L
Sbjct: 268 LGDL---YSIAWMEDSDIHNLRTETLHQQYELVKTRTASYNSYGSHVMQYGDIGLSKNNL 324
Query: 297 TDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
+ G + N ++ + +++ NQ +
Sbjct: 325 FTYLG----TNPANDNYTFVDENSLRPASKAVNQRDADL 359
>gb|AAK15049.1|AF238384_1 (AF238384) asparaginyl endopeptidase [Vigna radiata]
Length = 483
Score = 518 bits (1322), Expect = e-146
Identities = 80/349 (22%), Positives = 144/349 (40%), Gaps = 26/349 (7%)
Query: 4 LLSFILPIFLIFSTVLSSDSPNDSVNINNS---DPSRHSNNWAVLVSTSRFWFNYRHMAN 60
F+L + + D D + + + D + WA+L + S ++NYRH A+
Sbjct: 9 STLFLLFLATVALVAAGRDLVGDFLRLPSDSGNDDNVKGTRWAILFAGSNGYWNYRHQAD 68
Query: 61 TLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGY 120
Y+ +++ G+ + II+ + DDIA N N PG + N D D+Y E + DY G
Sbjct: 69 ICHAYQILRKGGLKEENIIVFMYDDIAFNWDNPRPGVIINKPD-GDDVY-EGVPKDYTGE 126
Query: 121 EVTVENFM-RLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDL 179
+ T NF LL DK K + S + IFI+ + HGG L I A DL
Sbjct: 127 DATAHNFYSALLGDKSALTGGSGKVVNSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDL 186
Query: 180 ADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHHS---- 233
+ + + Y + F ++ C+A +++E + NI A +S EESS+ +
Sbjct: 187 VEVLKKKHASGTYKNLVFYLEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEY 246
Query: 234 ---DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHS--HPGIRTDL 286
+ + D Y++ ++E DR + E++ + + + +
Sbjct: 247 PSPPPEYSTCLGDL---YSVAWMEDSDRHNLRTESLHQQYKVVKDRTLSGGWYGSHVMQY 303
Query: 287 FKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
S+ L + G + N+ ++ + S+ NQ +
Sbjct: 304 GDVEFSKDTLFLYLG----TDPANDNLTFVDENSLWSSSTAVNQRDADL 348
>emb|CAC18099.1| (AJ251453) putative legumain [Zea mays]
Length = 486
Score = 518 bits (1321), Expect = e-146
Identities = 75/353 (21%), Positives = 146/353 (41%), Gaps = 31/353 (8%)
Query: 5 LSFILPIFLIFSTVLSSDSPNDSVN------INNSDPSRHSNNWAVLVSTSRFWFNYRHM 58
LS +L + L + P + + +D WAVL++ S ++NYRH
Sbjct: 8 LSLLLSVCLSSAWARPRLEPAIRLPSQRAAAADETDDGDVGTRWAVLIAGSSGYYNYRHQ 67
Query: 59 ANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYR 118
A+ Y+ +K+ G+ D II+ + DDIA +P N PG + N+ + D+Y + DY
Sbjct: 68 ADICHAYQIMKKGGLKDENIIVFMYDDIAHSPENPRPGVIINHP-QGGDVYA-GVPKDYT 125
Query: 119 GYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAH 177
G +V V+NF + L +K K + S N +IF++ + HGG L +
Sbjct: 126 GRDVNVDNFFAVLLGNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGD 185
Query: 178 DLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKIH--SPNILAVGSSEIEESSYSHHS-- 233
DL D + + Y + F ++ C++ +++E + N+ A +S +ESS+ +
Sbjct: 186 DLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYATTASNADESSWGTYCPG 245
Query: 234 -----DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVH----SHPGI 282
+ + D Y++ ++E D + E++ + + S+
Sbjct: 246 EFPSPPPEYDTCLGDL---YSVAWMEDSDFHNLRTESLKQQYKLVKDRTAVHDTFSYGSH 302
Query: 283 RTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
++ L + G + +E+ + ++ NQ +
Sbjct: 303 VMQYGALELNVQRLFSYIG----TDPANDGNTFIEDNSLPSFSKAVNQRDADL 351
>ref|NP_180165.1| (NM_128154) putative vacuolar processing enzyme [Arabidopsis
thaliana]
sp|P49047|VPEA_ARATH VACUOLAR PROCESSING ENZYME, ALPHA-ISOZYME PRECURSOR (ALPHA-VPE)
pir||T02629 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
gb|AAC31241.1| (AC004747) vacuolar processing enzyme, alpha-isozyme precursor
(alpha-VPE) [Arabidopsis thaliana]
dbj|BAA09614.2| (D61393) alpha-VPE [Arabidopsis thaliana]
gb|AAL24163.1| (AY058055) At2g25940/F17H15.3 [Arabidopsis thaliana]
Length = 478
Score = 518 bits (1321), Expect = e-146
Identities = 86/356 (24%), Positives = 152/356 (42%), Gaps = 33/356 (9%)
Query: 1 MNRLLSFILPIFLIFSTV------LSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFN 54
M ++SF+ + + V L S + N D S WAVLV+ S ++N
Sbjct: 1 MTTVVSFLALFLFLVAAVSGDVIKLPSLASKFFRPTENDDDS---TKWAVLVAGSSGYWN 57
Query: 55 YRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIE 114
YRH A+ Y+ +K+ G+ + I++ + DDIA N N PG + N+ + D+Y +
Sbjct: 58 YRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRPGVIINSPN-GEDVY-NGVP 115
Query: 115 VDYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEE 173
DY G EV V+N + + L +K K + S N +IFIY + HGG L +
Sbjct: 116 KDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPN 175
Query: 174 ISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSH 231
+ A+DL D + Y Y + F ++ C++ +++E + NI A +S EESS+
Sbjct: 176 LYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGT 235
Query: 232 HS-------DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENV---HSH 279
+ + + D Y++ ++E ++ + ET+ + + S+
Sbjct: 236 YCPGEDPSPPSEYETCLGDL---YSVAWIEDSEKHNLQTETLHEQYELVKKRTAGSGKSY 292
Query: 280 PGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
+ +S+ L F G D N + + + NQ +
Sbjct: 293 GSHVMEFGDIGLSKEKLVLFMG----TNPADENFTFVNENSIRPPSRVTNQRDADL 344
>dbj|BAA76745.1| (D89972) asparaginyl endopeptidase (VmPE-1A) [Vigna mungo]
Length = 482
Score = 516 bits (1317), Expect = e-145
Identities = 80/349 (22%), Positives = 144/349 (40%), Gaps = 26/349 (7%)
Query: 4 LLSFILPIFLIFSTVLSSDSPNDSVNINNS---DPSRHSNNWAVLVSTSRFWFNYRHMAN 60
F+L + + D D + + + D + WA+L + S ++NYRH A+
Sbjct: 8 STLFLLFLATVALVAARRDHVGDFLRLPSDSGNDDNVQGTRWAILFAGSNGYWNYRHQAD 67
Query: 61 TLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGY 120
Y+ +++ G+ + II+ + DDIA N N PG + N D D+Y E + DY G
Sbjct: 68 ICHAYQILRKGGLKEENIIVFMYDDIAFNWDNPRPGVIINKPD-GDDVY-EGVPKDYTGE 125
Query: 121 EVTVENFM-RLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDL 179
+ T NF LL DK K + S + IFI+ + HGG L I A DL
Sbjct: 126 DATAHNFYSALLGDKSALTGGSGKVVSSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDL 185
Query: 180 ADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHHS---- 233
+ + + Y + F ++ C+A +++E + NI A +S EESS+ +
Sbjct: 186 VEVLKKKHASGTYKNLVFYLEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEY 245
Query: 234 ---DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHS--HPGIRTDL 286
+ + D Y++ ++E DR + E++ + + + +
Sbjct: 246 PSPPPEYSTCLGDL---YSVAWMEDSDRHNLRTESLHQQYKVVKDRTLSGGWYGSHVMQY 302
Query: 287 FKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
S+ L + G + N+ ++ + S+ NQ +
Sbjct: 303 GDVEFSKDALFLYLG----TDPANDNLTFVDENSLWSSSTAVNQRDADL 347
>emb|CAB64545.1| (AJ131719) legumain-like protease [Zea mays]
Length = 481
Score = 516 bits (1316), Expect = e-145
Identities = 72/348 (20%), Positives = 144/348 (40%), Gaps = 26/348 (7%)
Query: 5 LSFILPIFLIFSTVLSSDSPNDSVNIN-NSDPSRHSNNWAVLVSTSRFWFNYRHMANTLS 63
L+ +L L + P + + + WAVL++ S ++NYRH A+
Sbjct: 8 LALLLSACLCSAWARPRLEPTIRLPSDRAAADDAVGTRWAVLIAGSNGYYNYRHQADICH 67
Query: 64 LYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVT 123
Y+ +K+ G+ D I++ + DDIA +P N PG + N+ + D+Y + DY G +V
Sbjct: 68 AYQIMKKGGLKDENIVVFMYDDIAHSPENPRPGVIINHP-QGGDVYA-GVPKDYTGRDVN 125
Query: 124 VENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADA 182
V+NF + L +K K + S N +I ++ + HGG L + DL D
Sbjct: 126 VDNFFAVLLGNKTALRGGSGKVVDSGPNDHISVFYSDHGGPGVLGMPTYPYLYGDDLVDV 185
Query: 183 FSQMYDQKRYNEIFFMIDTCQANTMYEKIH--SPNILAVGSSEIEESSYSHHS------- 233
+ + Y + F ++ C++ +++E + N+ A +S EESS+ +
Sbjct: 186 LKKKHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSP 245
Query: 234 DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVH----SHPGIRTDLF 287
+ + D Y++ ++E D + E++ + + S+
Sbjct: 246 PPEYDTCLGDL---YSVAWMEDSDFHNLRTESLKQQYNLVKDRTAVQDTFSYGSHVMQYG 302
Query: 288 KRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
++ L + G + + +E+ + ++ NQ +
Sbjct: 303 SLGLNVKHLFSYIG----TNPANDDNTFIEDNSLPSFSKAVNQRDADL 346
>gb|AAF69014.1|AF260827_1 (AF260827) cysteine protease [Ipomoea batatas]
Length = 492
Score = 514 bits (1312), Expect = e-145
Identities = 74/342 (21%), Positives = 136/342 (39%), Gaps = 26/342 (7%)
Query: 11 IFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVKR 70
+ S P + N WAVL++ S ++NYRH A+ Y+ +K
Sbjct: 25 FLKLPSEARRFFRPAEEENREADGDDSVGTRWAVLIAGSNGYWNYRHQADICHAYQILKA 84
Query: 71 LGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMRL 130
G+ D I++ + DDIA N N G + N+ D+Y + DY G +VT N + +
Sbjct: 85 GGLKDENIVVFMYDDIAYNEENPRKGIIINSP-HGEDVYH-GVPKDYTGDDVTANNLLAV 142
Query: 131 -LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQ 189
L DK K + S N +IFIY + HGG L + + A +L A + +
Sbjct: 143 ILGDKSAVKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPYLYADELNAALKKKHAA 202
Query: 190 KRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHHS-------DMDIGVA 240
Y + F ++ C++ +++E I NI A +S ESS+ + +
Sbjct: 203 GAYKSLVFYLEACESGSIFEGILPKDINIYATTASNAIESSWGTYCPGEYPSPPPEYETC 262
Query: 241 VIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHSH---PGIRTDLFKRNVSEVL 295
+ D Y++ ++E D + E++ + + ++ + ++
Sbjct: 263 LGDL---YSIAWMEDSDIHNLRTESLKQQYNLVKDRTLNGNTAYGSHVMQYGDLELNADS 319
Query: 296 LTDFFGNVQNVVVDDVNMELLENEKVTESNE--DNNQTKNII 335
L + G + N ++ + + S NQ +
Sbjct: 320 LFMYMG----TNPANENFTFVDEKSLKLSAPRRAVNQRDADL 357
>pir||S60049 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
Length = 478
Score = 513 bits (1308), Expect = e-144
Identities = 86/356 (24%), Positives = 153/356 (42%), Gaps = 33/356 (9%)
Query: 1 MNRLLSFILPIFLIFSTV------LSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFN 54
M ++SF+ + + V L S + N D S WAVLV+ S ++N
Sbjct: 1 MTTVVSFLALFLFLVAAVSGDVIKLPSLASKFFRPTENDDDS---TKWAVLVAGSSGYWN 57
Query: 55 YRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIE 114
YRH A+ Y+ +K+ G+ + I++++ DDIA N N PG + N+ + D+Y +
Sbjct: 58 YRHQADVCHAYQRLKKGGVKEENIVVLMYDDIAENEENPRPGVIINSPN-GEDVY-NGVP 115
Query: 115 VDYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEE 173
DY G EV V+N + + L +K K + S N +IFIY + HGG L +
Sbjct: 116 KDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPN 175
Query: 174 ISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSH 231
+ A+DL D + Y Y + F ++ C++ +++E + NI A +S EESS+
Sbjct: 176 LYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGT 235
Query: 232 HS-------DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENV---HSH 279
+ + + D Y++ ++E ++ + ET+ + + S+
Sbjct: 236 YCPGEDPSPPSEYETCLGDL---YSVAWIEDSEKHNLQTETLHEQYELVKKRTAGSGKSY 292
Query: 280 PGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
+ +S+ L F G D N + + + NQ +
Sbjct: 293 GSHVMEFGDIGLSKEKLVLFMG----TNPADENFTFVNENSIRPPSRVTNQRDADL 344
>ref|XP_039644.1| (XM_039644) phosphatidylinositol glycan, class K [Homo sapiens]
gb|AAB81597.1| (AF022913) GPI transamidase [Homo sapiens]
gb|AAH20737.1|AAH20737 (BC020737) Unknown (protein for MGC:22559) [Homo sapiens]
Length = 395
Score = 512 bits (1307), Expect = e-144
Identities = 174/348 (50%), Positives = 244/348 (70%), Gaps = 6/348 (1%)
Query: 1 MNRLLSFILPIFLIFSTVLSSDSPNDSVNINNSDP----SRHSNNWAVLVSTSRFWFNYR 56
M S ++ + +L S + +I + S H+NNWAVLV TSRFWFNYR
Sbjct: 1 MAVTDSLSRAATVLATVLLLSFGSVAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYR 60
Query: 57 HMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVD 116
H+ANTLS+YR+VKRLGIPDS I+LML+DD+ACNPRN P +VF++ + +++YG+ +EVD
Sbjct: 61 HVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPRNPKPATVFSHKNMELNVYGDDVEVD 120
Query: 117 YRGYEVTVENFMRLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISA 176
YR YEVTVENF+R+LT + PRSKRLLSD+ SNI IY+TGHGGN FLKFQD+EEI+
Sbjct: 121 YRSYEVTVENFLRVLTGRIPPSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITN 180
Query: 177 HDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKIHSPNILAVGSSEIEESSYSHHSDMD 236
+LADAF QM+ ++RYNE+ F+IDTCQ +MYE+ +SPNI+A+ SS++ E S SH D
Sbjct: 181 IELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPA 240
Query: 237 IGVAVIDRFTYYTLDFLEKIDRDSKETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLL 296
IGV ++DR+T+Y L+FLE+I+ S+ M+ LF S PG RTDLF+R+ VL+
Sbjct: 241 IGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVCPKSLCVSTPGHRTDLFQRDPKNVLI 300
Query: 297 TDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNIIKERGPTQFS 344
TDFFG+V+ V + ++L ++ ++ ES+ +Q + E P +++
Sbjct: 301 TDFFGSVRKVEITTETIKLQQDSEIMESSYKEDQMDEKLME--PLKYA 346
>dbj|BAA76744.1| (D89971) asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
Length = 483
Score = 512 bits (1307), Expect = e-144
Identities = 72/331 (21%), Positives = 141/331 (41%), Gaps = 25/331 (7%)
Query: 20 SSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVKRLGIPDSQII 79
S++ +D + WAVL++ S ++NYRH ++ Y+ + + G+ + I+
Sbjct: 28 PSEASRFFQ-APATDENDEGTRWAVLIAGSNGYWNYRHQSDVCHAYQLLTKGGLKEENIV 86
Query: 80 LMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFM-RLLTDKWDSD 138
+ + DDIA N N PG + N+ D+Y + + DY G +VTV NF +L +K
Sbjct: 87 VFMYDDIAFNEENPRPGVIINSP-HGNDVY-KGVPKDYVGEDVTVNNFFAAILGNKSALT 144
Query: 139 QPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQKRYNEIFFM 198
K + S N +IFIY + HGG L + + A DL + + + Y + F
Sbjct: 145 GGSGKVVNSGPNDHIFIYYSDHGGPGVLGMPTSPYMYASDLIEVLKKKHASGTYKSLAFY 204
Query: 199 IDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHHS-------DMDIGVAVIDRFTYYT 249
++ C++ +++ + NI A ++ EESS+ + + + D Y+
Sbjct: 205 LEGCESGSIFGGLLPEGLNIYATTAANAEESSWGTYCPGDNPSPPPEYETCLGDL---YS 261
Query: 250 LDFLEKIDRDS--KETMDKLFAEYTFENVHSH---PGIRTDLFKRNVSEVLLTDFFGNVQ 304
+ ++E D + ET+ + F ++ + +S+ ++ + G
Sbjct: 262 VAWMEDSDIHNLRTETLHQQFELVKQRTMNGNSAYGSHVMQYGDVGLSKNNVSLYLG--- 318
Query: 305 NVVVDDVNMELLENEKVTESNEDNNQTKNII 335
+ N E + ++ NQ +
Sbjct: 319 -TNPANDNFPFREKNSLVPPSKAVNQRDADL 348
>gb|AAD04883.1| (AF082347) C13 endopeptidase NP1 precursor [Zea mays]
Length = 485
Score = 512 bits (1306), Expect = e-144
Identities = 74/352 (21%), Positives = 145/352 (41%), Gaps = 30/352 (8%)
Query: 5 LSFILPIFLIFSTVLSSDSPNDSVNINN-----SDPSRHSNNWAVLVSTSRFWFNYRHMA 59
L+ +L L + P + +D WAVL++ S ++NYRH A
Sbjct: 8 LALLLSACLCSAWARPRLEPTIRLPSERAAADETDDDAVGTRWAVLIAGSNGYYNYRHQA 67
Query: 60 NTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRG 119
+ Y+ +K+ G+ D I++ + DDIA +P N PG + N+ + D+Y + DY G
Sbjct: 68 DICHAYQIMKKGGLKDENIVVFMYDDIAHSPENPRPGVIINHP-QGGDVYA-GVPKDYTG 125
Query: 120 YEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHD 178
+V V+NF + L +K K + S N +IF++ + HGG L + D
Sbjct: 126 RDVNVDNFFAVLLGNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDD 185
Query: 179 LADAFSQMYDQKRYNEIFFMIDTCQANTMYEKIH--SPNILAVGSSEIEESSYSHHS--- 233
L D + + Y + F ++ C++ +++E + N+ A +S EESS+ +
Sbjct: 186 LVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGE 245
Query: 234 ----DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVH----SHPGIR 283
+ + D Y++ ++E D + E++ + + S+
Sbjct: 246 FPSPPPEYDTCLGDL---YSVAWMEDSDFHNLRTESLKQQYNLVKDRTAVQDTFSYGSHV 302
Query: 284 TDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
++ L + G + + +E+ + ++ NQ +
Sbjct: 303 MQYGSLELNVKHLFSYIG----TNPANDDNTFIEDNSLPSLSKAVNQRDADL 350
>pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17078.1| (Z99956) asparagine-specific endopeptidase precursor [Phaseolus
vulgaris]
Length = 484
Score = 511 bits (1303), Expect = e-143
Identities = 79/352 (22%), Positives = 151/352 (42%), Gaps = 27/352 (7%)
Query: 2 NRLLSFILPIFLIFSTVLSSDSPNDSVNINNSD---PSRHSNNWAVLVSTSRFWFNYRHM 58
L +L +FL+ D D + + + + H WA+L + S ++NYRH
Sbjct: 7 TTSLLALLLLFLVALVSAGRDLVGDFLRLPSDSGNGDNVHGTRWAILFAGSSGYWNYRHQ 66
Query: 59 ANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYR 118
A+ Y+ +++ G+ D II+ + DDIA N N G + N+ + ++Y + + DY
Sbjct: 67 ADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVIINSPN-GDEVY-KGVPKDYT 124
Query: 119 GYEVTVENFM-RLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAH 177
G +VT NF LL DK K + S N +IFI+ + HGG L I A
Sbjct: 125 GEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYAS 184
Query: 178 DLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHHS-- 233
DL + + + Y + F ++ C++ +++E + N+ A +S +ESS+ +
Sbjct: 185 DLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVYATTASNADESSWGTYCPG 244
Query: 234 -----DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVH---SHPGIR 283
+ + D Y++ ++E DR + ET+ + + + +
Sbjct: 245 EDPSPPPEYSTCLGDL---YSVAWMEDSDRHNLRTETLHQQYKLVKERTISGGLYYGSHV 301
Query: 284 TDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
+S+ +L + G + N+ ++ + S++ NQ +
Sbjct: 302 MQYGDVGLSKDILFHYLG----TDPANENLTFVDENSLWSSSKAVNQRDADL 349
>emb|CAC18100.1| (AJ251454) putative legumain [Zea mays]
Length = 485
Score = 509 bits (1299), Expect = e-143
Identities = 75/352 (21%), Positives = 145/352 (40%), Gaps = 30/352 (8%)
Query: 5 LSFILPIFLIFSTVLSSDSPNDSVNINN-----SDPSRHSNNWAVLVSTSRFWFNYRHMA 59
L+ +L L + P + +D WAVL++ S ++NYRH A
Sbjct: 8 LALLLSACLCSAWARPRLEPTIRLPSERAAADETDDDAVGTRWAVLIAGSNGYYNYRHQA 67
Query: 60 NTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRG 119
+ Y+ +K+ G+ D I++ + DDIA +P N PG + N+ + D+Y + DY G
Sbjct: 68 DICHAYQIMKKGGLKDENIVVFMYDDIAHSPENPRPGVIINHP-QGGDVYA-GVPKDYTG 125
Query: 120 YEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHD 178
EV V+NF + L +K K + S N +IF++ + HGG L + D
Sbjct: 126 REVNVDNFFAVLLGNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDD 185
Query: 179 LADAFSQMYDQKRYNEIFFMIDTCQANTMYEKIH--SPNILAVGSSEIEESSYSHHS--- 233
L D + + Y + F ++ C++ +++E + N+ A +S EESS+ +
Sbjct: 186 LVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGE 245
Query: 234 ----DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVH----SHPGIR 283
+ + D Y++ ++E D + E++ + + S+
Sbjct: 246 FPSPPPEYDTCLGDL---YSVAWMEDSDFHNLRTESLKQQYNLVKDRTAVQDTFSYGSHV 302
Query: 284 TDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
++ L + G + + +E+ + ++ NQ +
Sbjct: 303 MQYGSLELNVKHLFSYIG----TNPANDDNTSIEDNSLPSFSKAVNQRDADL 350
>emb|CAA68871.1| (Y07596) gpi8 [Homo sapiens]
Length = 396
Score = 507 bits (1293), Expect = e-142
Identities = 173/343 (50%), Positives = 243/343 (70%), Gaps = 6/343 (1%)
Query: 6 SFILPIFLIFSTVLSSDSPNDSVNINNSDP----SRHSNNWAVLVSTSRFWFNYRHMANT 61
S ++ + +L S + +I + S H+NNWAVLV TSRFWFNYRH+ANT
Sbjct: 7 SLSRAATVLATVLLLSFGSVAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANT 66
Query: 62 LSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYE 121
LS+YR+VKRLGIPDS I+LML+DD+ACNPRN P +VF++ + +++YG+ +EVDYR YE
Sbjct: 67 LSVYRSVKRLGIPDSHIVLMLADDMACNPRNPKPATVFSHKNMELNVYGDDVEVDYRSYE 126
Query: 122 VTVENFMRLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLAD 181
VTVENF+R+LT + PRSKRLLSD+ SNI IY+TGHGGN FLKFQD+EEI+ +LAD
Sbjct: 127 VTVENFLRVLTGRIPPSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELAD 186
Query: 182 AFSQMYDQKRYNEIFFMIDTCQANTMYEKIHSPNILAVGSSEIEESSYSHHSDMDIGVAV 241
AF QM+ ++RYNE+ F+IDTCQ +MYE+ +SPNI+A+ SS++ E S SH D IGV +
Sbjct: 187 AFEQMWQKRRYNELLFIIDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHL 246
Query: 242 IDRFTYYTLDFLEKIDRDSKETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFG 301
+DR+T+Y L+FLE+I+ S+ M+ LF S PG RTDLF+R+ VL+TDFFG
Sbjct: 247 MDRYTFYVLEFLEEINPASQTNMNDLFQVCPKSLCVSTPGHRTDLFQRDPKNVLITDFFG 306
Query: 302 NVQNVVVDDVNMELLENEKVTESNEDNNQTKNIIKERGPTQFS 344
+V+ V + ++L ++ ++ ES+ +Q + E P +++
Sbjct: 307 SVRKVEITTETIKLQQDSEIMESSYKEDQMDEKLME--PLKYA 347
>sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE)
pir||JQ2387 vacuolar processing enzyme (EC 3.4.22.-) precursor - castor bean
dbj|BAA04225.1| (D17401) precursor of vacuolar processing enzyme [Ricinus communis]
Length = 497
Score = 507 bits (1292), Expect = e-142
Identities = 73/339 (21%), Positives = 135/339 (39%), Gaps = 27/339 (7%)
Query: 14 IFSTVLSSDSPN-DSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVKRLG 72
+ P ++ + D + WAVLV+ S + NYRH A+ Y+ +++ G
Sbjct: 34 LNPFEPGILMPTEEAEPVQVDDDDQLGTRWAVLVAGSMGFGNYRHQADVCHAYQLLRKGG 93
Query: 73 IPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMRL-L 131
+ + II+ + DDIA N N PG + N+ + D+Y + DY G VT +N + L
Sbjct: 94 LKEENIIVFMYDDIAKNELNPRPGVIINHP-QGEDVYA-GVPKDYTGEHVTAKNLYAVLL 151
Query: 132 TDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQKR 191
DK K + S N IF+Y + HGG L + + A D + + +
Sbjct: 152 GDKSAVQGGSGKVVDSKPNDRIFLYYSDHGGPGVLGMPNLPYLYAMDFIEVLKKKHAAGG 211
Query: 192 YNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHHS-------DMDIGVAVI 242
Y ++ ++ C++ +++E I +I +S +ESS+ + + +
Sbjct: 212 YKKMVIYVEACESGSIFEGIMPKDVDIYVTTASNAQESSWGTYCPGMEPSPPPEFTTCLG 271
Query: 243 DRFTYYTLDFLEK--IDRDSKETMDKLFAEYTFENVHSH----PGIRTDLFKRNVSEVLL 296
D Y++ ++E KET+ + ++ + + +++ L
Sbjct: 272 DL---YSVAWMEDSESHNLKKETVKQQYSSVKARTSNYNTYAAGSHVMQYGNQSIKADKL 328
Query: 297 TDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
F G VN N + E NQ +
Sbjct: 329 YLFQG----FDPASVNFP-PNNAHLNAPMEVVNQRDAEL 362
>gb|AAF89679.1| (AF169973) asparaginyl endopeptidase [Sesamum indicum]
Length = 489
Score = 504 bits (1285), Expect = e-141
Identities = 74/356 (20%), Positives = 142/356 (39%), Gaps = 33/356 (9%)
Query: 3 RLLSFILPIFLIFSTVLSSDSPNDSV-------NINNSDPSRHSNNWAVLVSTSRFWFNY 55
RL+ +L + + + ++ + ++ WAVLV+ S + NY
Sbjct: 9 RLMILVLCVVVALPFAAAGGGRRSGPWDPIIRWPLDRRETEDNATRWAVLVAGSNGFGNY 68
Query: 56 RHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEV 115
RH A+ Y+ +K+ G+ D II+ + DDIA N N G + N+ D+Y +
Sbjct: 69 RHQADVCHAYQILKKGGLRDENIIVFMYDDIAMNELNPRKGVIINHPT-GGDVYA-GVPK 126
Query: 116 DYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEI 174
DY G +VT EN + L DK K + S N IFIY + HGG L + +
Sbjct: 127 DYTGEQVTAENLYAVILGDKSAIKGGSGKVVDSKPNDRIFIYYSDHGGPGVLGMPNMPYL 186
Query: 175 SAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHH 232
A+D + + + Y E+ ++ C++ +++E + +I +S EESS+ +
Sbjct: 187 YANDFIEVLKKKHASGTYKEMVIYVEACESGSVFEGLMPDDLDIYVTTASNAEESSWGTY 246
Query: 233 SD-------MDIGVAVIDRFTYYTLDFLEK--IDRDSKETMDKLFAEYTFENVHSH---- 279
+ + D Y++ ++E +ET+++ + + + +
Sbjct: 247 CPGMDPPPPPEYITCLGDL---YSVAWMEDSESHNLKRETVEQQYQQVKERTSNFNTYNA 303
Query: 280 PGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
+ +++ L + G NM + + NQ +
Sbjct: 304 GSHVMEYGNKSIKSEKLYLYQG----FDPATENMP-PSENHLKPHMDVVNQRDADL 354
>ref|NP_195020.1| (NM_119448) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
pir||T05302 vacuolar processing enzyme (EC 3.4.22.-) isozyme gamma precursor -
Arabidopsis thaliana
emb|CAA21203.1| (AL031804) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
emb|CAB80011.1| (AL161582) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
gb|AAK43975.1|AF370160_1 (AF370160) putative gamma-VPE protein [Arabidopsis thaliana]
gb|AAL11612.1|AF424619_1 (AF424619) AT4g32940/F26P21_60 [Arabidopsis thaliana]
gb|AAL15210.1| (AY059104) putative gamma-VPE protein [Arabidopsis thaliana]
Length = 494
Score = 502 bits (1281), Expect = e-141
Identities = 82/364 (22%), Positives = 149/364 (40%), Gaps = 38/364 (10%)
Query: 1 MNRLLSFILPIFLIFSTV--------------LSSDSPNDSVNINNSDPSRHSNNWAVLV 46
M R+ ++ L+ S V L S + N D S WAVLV
Sbjct: 5 MTRVSVGVVLFVLLVSLVAVSAARSGPDDVIKLPSQASRFFRPAENDDDSNSGTRWAVLV 64
Query: 47 STSRFWFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAI 106
+ S ++NYRH A+ Y+ +++ G+ + I++ + DDIA N N PG++ N+
Sbjct: 65 AGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTIINSP-HGK 123
Query: 107 DLYGESIEVDYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEF 165
D+Y + + DY G +V V+N + L DK K + S N +IFI+ + HGG
Sbjct: 124 DVY-QGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGV 182
Query: 166 LKFQDAEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSE 223
L + + A+DL D + + Y + F ++ C++ +++E + NI A +S
Sbjct: 183 LGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASN 242
Query: 224 IEESSYSHHS-------DMDIGVAVIDRFTYYTLDFLEKI--DRDSKETMDKLFAEYTFE 274
EESS+ + + + D Y++ ++E ET+ + +
Sbjct: 243 AEESSWGTYCPGEEPSPPPEYETCLGDL---YSVAWMEDSGMHNLQTETLHQQYELVKRR 299
Query: 275 NV---HSHPGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQT 331
+S+ +S+ L + G + N + + + NQ
Sbjct: 300 TAPVGYSYGSHVMQYGDVGISKDNLDLYMG----TNPANDNFTFADANSLKPPSRVTNQR 355
Query: 332 KNII 335
+
Sbjct: 356 DADL 359
>sp|Q39119|VPEG_ARATH VACUOLAR PROCESSING ENZYME, GAMMA-ISOZYME PRECURSOR (GAMMA-VPE)
dbj|BAA18924.1| (D61395) gamma-VPE [Arabidopsis thaliana]
Length = 490
Score = 502 bits (1281), Expect = e-141
Identities = 82/364 (22%), Positives = 149/364 (40%), Gaps = 38/364 (10%)
Query: 1 MNRLLSFILPIFLIFSTV--------------LSSDSPNDSVNINNSDPSRHSNNWAVLV 46
M R+ ++ L+ S V L S + N D S WAVLV
Sbjct: 1 MTRVSVGVVLFVLLVSLVAVSAARSGPDDVIKLPSQASRFFRPAENDDDSNSGTRWAVLV 60
Query: 47 STSRFWFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAI 106
+ S ++NYRH A+ Y+ +++ G+ + I++ + DDIA N N PG++ N+
Sbjct: 61 AGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTIINSP-HGK 119
Query: 107 DLYGESIEVDYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEF 165
D+Y + + DY G +V V+N + L DK K + S N +IFI+ + HGG
Sbjct: 120 DVY-QGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGV 178
Query: 166 LKFQDAEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSE 223
L + + A+DL D + + Y + F ++ C++ +++E + NI A +S
Sbjct: 179 LGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASN 238
Query: 224 IEESSYSHHS-------DMDIGVAVIDRFTYYTLDFLEKI--DRDSKETMDKLFAEYTFE 274
EESS+ + + + D Y++ ++E ET+ + +
Sbjct: 239 AEESSWGTYCPGEEPSPPPEYETCLGDL---YSVAWMEDSGMHNLQTETLHQQYELVKRR 295
Query: 275 NV---HSHPGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQT 331
+S+ +S+ L + G + N + + + NQ
Sbjct: 296 TAPVGYSYGSHVMQYGDVGISKDNLDLYMG----TNPANDNFTFADANSLKPPSRVTNQR 351
Query: 332 KNII 335
+
Sbjct: 352 DADL 355
>emb|CAC43295.1| (AJ309173) putative vacuolar processing enzyme [Beta vulgaris]
Length = 486
Score = 500 bits (1274), Expect = e-140
Identities = 82/359 (22%), Positives = 153/359 (41%), Gaps = 33/359 (9%)
Query: 1 MNRLLSFILPIFLIFS--TVLSSDS-------PNDSVNINNSDPSRHSNNWAVLVSTSRF 51
M R S +L + + V S P+D +I SD WAVL++ S
Sbjct: 2 MIRYTSGVLIVLCVLMSSVVDSRLMVDNLIRWPSDHPSIFESDDDSVGTRWAVLIAGSSG 61
Query: 52 WFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGE 111
++NYRH A+ Y+ +K+ G+ D II+ + DDIA + N PG + N+ D+Y
Sbjct: 62 YWNYRHQADVCHAYQVLKKGGLKDENIIVFMYDDIAYDEENPRPGVLINSP-YGHDVYA- 119
Query: 112 SIEVDYRGYEVTVENFM-RLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQD 170
+ DY G +VTV NF +L +K K + S N +IFI+ + HGG L
Sbjct: 120 GVPKDYTGEDVTVNNFFAAILGNKDAITGGSGKVVNSGPNDHIFIFYSDHGGAGVLGMPT 179
Query: 171 AEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESS 228
+ A +L + + + Y + I+ C++ +++E I NI A +S ESS
Sbjct: 180 YPYLYADELIETLKEKHASGTYKSLVVYIEACESGSIFEGILPEGLNIYATTASNAVESS 239
Query: 229 YSHHS-------DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVH-- 277
+ + + + D Y++ ++E +R + E++ + + +
Sbjct: 240 WGTYCPGQDPNVPPEYDTCLGDL---YSVSWIEDSERHNLHTESLKQQYEVVKTKTAEKP 296
Query: 278 SHPGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKV-TESNEDNNQTKNII 335
+ + +++ +L + G ++ N +++ + S+ NQ +
Sbjct: 297 FYGSHVMQYGDKELTQDMLYLYMG----TNPNNENYTYVDDNSLHPTSSNAVNQRDADL 351
>dbj|BAA84650.1| (AB025310) asparaginyl endopeptidase [Oryza sativa]
dbj|BAB85400.1| (AP003924) asparaginyl endopeptidase [Oryza sativa (japonica
cultivar-group)]
Length = 501
Score = 499 bits (1272), Expect = e-140
Identities = 76/363 (20%), Positives = 147/363 (39%), Gaps = 40/363 (11%)
Query: 4 LLSFILPIFLIFSTVLSS-----DSPNDSVNINNSD----------PSRHSNNWAVLVST 48
L+ +L + + ++ + N + + + WAVL++
Sbjct: 13 PLAALLLFAHLAAVAVARPRWEEEGSNLRLPSERAVAAGAAADDAAEAAEGTRWAVLIAG 72
Query: 49 SRFWFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDL 108
S ++NYRH A+ Y+ +KR G+ D II+ + DDIA NP N PG + N+ + D+
Sbjct: 73 SNGYYNYRHQADVCHAYQIMKRGGLKDENIIVFMYDDIAHNPENPRPGVIINHP-QGGDV 131
Query: 109 YGESIEVDYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLK 167
Y + DY G EV V+N + L +K K L S N +IFI+ + HGG L
Sbjct: 132 YA-GVPKDYTGKEVNVKNLFAVLLGNKTAVKGGSGKVLDSGPNDHIFIFYSDHGGPGVLG 190
Query: 168 FQDAEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKIH--SPNILAVGSSEIE 225
+ DL D + + Y + F ++ C++ +++E + N+ A +S +
Sbjct: 191 MPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNGINVYATTASNAD 250
Query: 226 ESSYSHHS-------DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENV 276
ESS+ + + + D Y++ ++E D + E++ + +
Sbjct: 251 ESSWGTYCPGEYPSPPPEYDTCLGDL---YSVAWMEDSDVHNLRTESLKQQYNLVKERTS 307
Query: 277 HSH----PGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTK 332
H + ++ + + G+ + N +E+ + + NQ
Sbjct: 308 VQHTYYSGSHVMEYGSLELNAHHVFMYMGS----NPANDNATFVEDNSLPSFSRAVNQRD 363
Query: 333 NII 335
+
Sbjct: 364 ADL 366
>sp|P49045|VPE_SOYBN VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||T07132 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - soybean
dbj|BAA06030.1| (D28876) cysteine proteinase [Glycine max]
Length = 495
Score = 498 bits (1271), Expect = e-140
Identities = 73/320 (22%), Positives = 129/320 (39%), Gaps = 26/320 (8%)
Query: 32 NSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPR 91
++D WAVLV+ S + NYRH A+ Y+ + + G+ + I++ + DDIA N
Sbjct: 51 DADSDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKEENIVVFMYDDIATNEL 110
Query: 92 NAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDEN 150
N G + N+ E DLY + DY G VT EN + L DK K + S
Sbjct: 111 NPRHGVIINHP-EGEDLYA-GVPKDYTGDNVTTENLFAVILGDKSKLKGGSGKVINSKPE 168
Query: 151 SNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEK 210
IFIY + HGG L + + A D D + + Y E+ ++ C++ +++E
Sbjct: 169 DRIFIYYSDHGGPGILGMPNMPYLYAMDFIDVLKKKHASGSYKEMVIYVEACESGSVFEG 228
Query: 211 I--HSPNILAVGSSEIEESSYSHHS-------DMDIGVAVIDRFTYYTLDFLEKI--DRD 259
I NI +S +E+S+ + + + D Y++ ++E
Sbjct: 229 IMPKDLNIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGDL---YSVAWMEDSEAHNL 285
Query: 260 SKETMDKLFAEYTFENVHSH----PGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMEL 315
+E++ + + + + N++ L + G VN
Sbjct: 286 KRESVKQQYKSVKQRTSNFNNYAMGSHVMQYGDTNITAEKLYLYQG----FDPATVNFP- 340
Query: 316 LENEKVTESNEDNNQTKNII 335
+N ++ E NQ +
Sbjct: 341 PQNGRLETKMEVVNQRDAEL 360
>pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17079.1| (Z99957) legumain-like proteinase precursor [Phaseolus vulgaris]
Length = 493
Score = 498 bits (1270), Expect = e-140
Identities = 71/320 (22%), Positives = 129/320 (40%), Gaps = 26/320 (8%)
Query: 32 NSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPR 91
+++ WAVLV+ S + NYRH A+ Y+ + + G+ + I++ + DDIA +
Sbjct: 49 DAESDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIATHEL 108
Query: 92 NAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDEN 150
N PG + NN + D+Y + DY G VT NF + L DK K + S
Sbjct: 109 NPRPGVIINNP-QGPDVYA-GVPKDYTGESVTSHNFFAVLLGDKSKVKGGSGKVINSKPE 166
Query: 151 SNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEK 210
IF+Y + HGG L + + A D D + + Y E+ ++ C++ +++E
Sbjct: 167 DRIFVYYSDHGGPGVLGMPNMPYLYAMDFIDVLKKKHASGGYKEMVIYVEACESGSIFEG 226
Query: 211 I--HSPNILAVGSSEIEESSYSHHSD-------MDIGVAVIDRFTYYTLDFLEK--IDRD 259
I NI +S +E+S+ + + + D Y++ ++E
Sbjct: 227 IMPKDLNIYVTTASNAQENSWGTYCPGMYPPPPPEYITCLGDL---YSVAWMEDSESHNL 283
Query: 260 SKETMDKLFAEYTFENVHSH----PGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMEL 315
KE++++ + + N++ L + G VN
Sbjct: 284 KKESVEQQYQSVKQRTSNFEAYAMGSHVMQYGDANMTAEKLYLYHG----FDPATVNFP- 338
Query: 316 LENEKVTESNEDNNQTKNII 335
N ++ E NQ +
Sbjct: 339 PHNGRLKSKMEVVNQRDAEL 358
>sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptidase)
pir||JX0344 legumain (EC 3.4.22.34) precursor - jack bean
dbj|BAA06596.1| (D31787) asparaginyl endopeptidase (Legumain) [Canavalia
ensiformis]
Length = 475
Score = 496 bits (1265), Expect = e-139
Identities = 74/317 (23%), Positives = 129/317 (40%), Gaps = 26/317 (8%)
Query: 35 PSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAF 94
WAVLV+ S + NYRH A+ Y+ + + G+ + I++ + DDIA N N
Sbjct: 34 DDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIAYNAMNPR 93
Query: 95 PGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNI 153
PG + N+ + D+Y + DY G +VT EN + L DK K + S+ I
Sbjct: 94 PGVIINHP-QGPDVYA-GVPKDYTGEDVTPENLYAVILGDKSKVKGGSGKVINSNPEDRI 151
Query: 154 FIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI-- 211
FI+ + HGG L +A + A D D + + Y E+ I+ C++ +++E I
Sbjct: 152 FIFYSDHGGPGVLGMPNAPFVYAMDFIDVLKKKHASGGYKEMVIYIEACESGSIFEGIMP 211
Query: 212 HSPNILAVGSSEIEESSYSHHS-------DMDIGVAVIDRFTYYTLDFLEKI--DRDSKE 262
NI +S +E+S+ + + + D Y++ ++E +E
Sbjct: 212 KDLNIYVTTASNAQENSFGTYCPGMNPPPPEEYVTCLGDL---YSVSWMEDSETHNLKRE 268
Query: 263 TMDKLFAEYTFENVHSH----PGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLEN 318
T+ + + +S+ N++ L + G VN N
Sbjct: 269 TVQQQYQSVRKRTSNSNSYRFGSHVMQYGDTNITAEKLYLYHG----FDPATVNFP-PHN 323
Query: 319 EKVTESNEDNNQTKNII 335
+ E NQ +
Sbjct: 324 GNLEAKMEVVNQRDAEL 340
>sp|P49044|VPE_VICSA VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) (PROTEINASE B)
pir||S49175 legumain (EC 3.4.22.34) precursor [similarity] - spring vetch
pir||S68984 cysteine proteinase precursor - spring vetch
emb|CAA84383.1| (Z34899) cysteine proteinase [Vicia sativa]
Length = 493
Score = 494 bits (1261), Expect = e-139
Identities = 78/362 (21%), Positives = 148/362 (40%), Gaps = 36/362 (9%)
Query: 1 MNRLLSFILPIFLIFSTVLSSDSPNDSVNINNS----------DPSRHSNNWAVLVSTSR 50
++ LL F + + + D P D + + + D WA+L++ S
Sbjct: 6 LSTLLFFTIVVTFLTVVSSGRDLPGDYLRLPSETSRFFREPKNDDDFEGTRWAILLAGSN 65
Query: 51 FWFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYG 110
++NYRH ++ Y+ +++ G + II+ + DDIA N N PG + N D D+Y
Sbjct: 66 GYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDIASNEENPRPGVIINKPD-GDDVYA 124
Query: 111 ESIEVDYRGYEVTVENFM-RLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQ 169
+ DY G EV +NF LL +K K + S N +IF+Y T HGG L
Sbjct: 125 -GVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHIFVYYTDHGGPGVLGMP 183
Query: 170 DAEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEES 227
+ A DL + + + Y + F ++ C++ +++E + NI A +S EES
Sbjct: 184 VGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPDDLNIYATTASNAEES 243
Query: 228 SYSHHSD-------MDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFEN-VH 277
S+ ++ + + D Y++ ++E + + E++ + +
Sbjct: 244 SWGYYCPGDKPPPPPEYSTCLGDL---YSIAWMEDSEVHNLQTESLQQQYKLVKNRTISE 300
Query: 278 SHPGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENE----KVTESNEDNNQTKN 333
+ + +S+ L + G + N ++ K+ + NQ
Sbjct: 301 PYGSHVMEYGDIGLSKNDLYQYLG----TNPANDNNSFVDETENSLKLRTPSAAVNQRDA 356
Query: 334 II 335
+
Sbjct: 357 DL 358
>gb|AAF89646.1|AF169019_1 (AF169019) seed maturation protein PM40 [Glycine max]
Length = 496
Score = 491 bits (1252), Expect = e-138
Identities = 67/332 (20%), Positives = 128/332 (38%), Gaps = 27/332 (8%)
Query: 21 SDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVKRLGIPDSQIIL 80
S + ++ WAVLV+ S + NYRH A+ Y+ + + G+ + I++
Sbjct: 40 SVIKLPAEPVDADSDHEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKEENIVV 99
Query: 81 MLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMRL-LTDKWDSDQ 139
+ DDIA + N PG + N+ E D+Y + DY G VT +N + L DK
Sbjct: 100 FMYDDIATDELNPRPGVIINHP-EGQDVYA-GVPKDYTGENVTAQNLFAVILGDKNKVKG 157
Query: 140 PRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQKRYNEIFFMI 199
K + S IFIY + HGG L + + A D + + + Y ++ +
Sbjct: 158 GSGKVINSKPEDRIFIYYSDHGGPGVLGMPNMPYLYAMDFIEVLKKKHASGGYKKMVIYV 217
Query: 200 DTCQANT-MYEKI--HSPNILAVGSSEIEESSYSHHS-------DMDIGVAVIDRFTYYT 249
+ C++ + + I I +S +E+S+ + + + D Y+
Sbjct: 218 EACESGNHVLKGIMPKDLQIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGDL---YS 274
Query: 250 LDFLEKI--DRDSKETMDKLFAEYTFENVHSH----PGIRTDLFKRNVSEVLLTDFFGNV 303
+ ++E +E++ + + + + N++ L + G
Sbjct: 275 VAWMEDSETHNLKRESVKQQYKSVKQRTSNFNNYAMGSHVMQYGDTNITAEKLYLYQG-- 332
Query: 304 QNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
VN +N ++ E NQ +
Sbjct: 333 --FDPAAVNFP-PQNGRLETKMEVVNQRDAEL 361
>dbj|BAB29018.1| (AK013853) data source:SPTR, source key:O14822,
evidence:ISS~homolog to GPI TRANSAMIDASE~putative [Mus
musculus]
Length = 395
Score = 491 bits (1252), Expect = e-138
Identities = 169/348 (48%), Positives = 237/348 (67%), Gaps = 6/348 (1%)
Query: 1 MNRLLSFILPIFLIFSTVLSSDSPNDSVNINNSDP----SRHSNNWAVLVSTSRFWFNYR 56
M L + + + L S + + +I + S H+NNWAVLV TSRFWFNYR
Sbjct: 1 MAAPCFLTLRVATLAALALLSLGSSAAGHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYR 60
Query: 57 HMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVD 116
H+ANTLS+YR+VKRLGIPDS I+LML+DD+ACN RN P +VF++ + +++YG+ +EVD
Sbjct: 61 HVANTLSVYRSVKRLGIPDSHIVLMLADDMACNARNPKPATVFSHKNMELNVYGDDVEVD 120
Query: 117 YRGYEVTVENFMRLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISA 176
YR Y TVENF+R+LT + PRSKRLLSD+ SNI IY+TGHGGN FLKFQD+EEI+
Sbjct: 121 YRSYRGTVENFLRVLTXEVPPSTPRSKRLLSDDKSNILIYMTGHGGNGFLKFQDSEEITN 180
Query: 177 HDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKIHSPNILAVGSSEIEESSYSHHSDMD 236
+LADAF QM+ ++RYNE+ F+IDTCQ +MYE+ +SPNI+A+ SS++ E S SH D
Sbjct: 181 IELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPA 240
Query: 237 IGVAVIDRFTYYTLDFLEKIDRDSKETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLL 296
IGV ++DR+T+Y L+F ++I+ S+ M+ LF S PG RTDLF+R+ VL+
Sbjct: 241 IGVHLMDRYTFYVLEFWKEINPASQTNMNDLFQVCPKSLCVSTPGHRTDLFQRDPKNVLI 300
Query: 297 TDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNIIKERGPTQFS 344
TDFFG+V+ V + + L + +V +S+ + T GP +++
Sbjct: 301 TDFFGSVRKVEITTEKISLQWDSQVVDSSSKEDGTAEE--RMGPLKYA 346
>ref|NP_176458.1| (NM_104948) beta-VPE [Arabidopsis thaliana]
gb|AAK56243.1|AF367254_1 (AF367254) At1g62710/F23N19_8 [Arabidopsis thaliana]
gb|AAL15381.1| (AY059156) At1g62710/F23N19_8 [Arabidopsis thaliana]
Length = 486
Score = 488 bits (1245), Expect = e-137
Identities = 80/355 (22%), Positives = 142/355 (39%), Gaps = 31/355 (8%)
Query: 2 NRLLSFILPIFLIFS-----TVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYR 56
R +L + L+ + P + N + D WAVLV+ S + NYR
Sbjct: 7 FRPALLLLLVLLVHAESRGRFEPKILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYR 66
Query: 57 HMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVD 116
H A+ Y+ +++ G+ + I++++ DDIA +P N PG++ N+ D D+Y + D
Sbjct: 67 HQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINHPD-GDDVYA-GVPKD 124
Query: 117 YRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEIS 175
Y G VT NF + L D+ K + S N +IF+Y HGG L + I
Sbjct: 125 YTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIY 184
Query: 176 AHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHHS 233
A D + + + Y E+ ++ C++ +++E I NI +S +ESSY +
Sbjct: 185 AADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYC 244
Query: 234 -------DMDIGVAVIDRFTYYTLDFLEKI--DRDSKETMDKLFAEYTFENVHSH----P 280
+ + D Y++ ++E KET+ + + + +
Sbjct: 245 PGMNPSPPSEYITCLGDL---YSVAWMEDSETHNLKKETIKQQYHTVKMRTSNYNTYSGG 301
Query: 281 GIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
+ ++ L + G VN+ L V NQ +
Sbjct: 302 SHVMEYGNNSIKSEKLYLYQG----FDPATVNLPL-NELPVKSKIGVVNQRDADL 351
>pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme beta precursor -
Arabidopsis thaliana
dbj|BAA09615.1| (D61394) beta-VPE [Arabidopsis thaliana]
Length = 484
Score = 479 bits (1222), Expect = e-134
Identities = 79/357 (22%), Positives = 145/357 (40%), Gaps = 37/357 (10%)
Query: 2 NRLLSFILPIFLIFS-----TVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYR 56
R +L + L+ + P + N + D WAVLV+ S + NYR
Sbjct: 7 FRPALLLLLVLLVHAESRGRFEPKILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYR 66
Query: 57 HMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVD 116
H A+ Y+ +++ G+ + I++++ DDIA +P N PG++ N+ D D+Y + D
Sbjct: 67 HQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINHPD-GDDVYA-GVPKD 124
Query: 117 YRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEIS 175
Y G VT NF + L D+ K + S N +IF+Y HGG L + I
Sbjct: 125 YTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIY 184
Query: 176 AHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHHS 233
A D + + + Y E+ ++ C++ +++E I NI +S +ESSY +
Sbjct: 185 AADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYC 244
Query: 234 -------DMDIGVAVIDRFTYYTLDFLEKI--DRDSKETMDKLFAEYTFENVHSH----P 280
+ + D Y++ ++E KET+ + + + +
Sbjct: 245 PGMNPSPPSEYITCLGDL---YSVAWMEDSETHNLKKETIKQQYHTM--RTSNYNTYSGG 299
Query: 281 GIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNE--DNNQTKNII 335
+ ++ L + G VN+ L ++ ++ NQ +
Sbjct: 300 SHVMEYGNNSIKSEKLYLYQG----FEPATVNLPL---NELPAKSKIGVVNQRDADL 349
>pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring vetch
emb|CAA07639.1| (AJ007743) cysteine proteinase precursor [Vicia sativa]
Length = 503
Score = 479 bits (1221), Expect = e-134
Identities = 75/364 (20%), Positives = 145/364 (39%), Gaps = 39/364 (10%)
Query: 1 MNRLLSFILPIFLIFSTVLSSDSPN------------DSVNINNSDPSRHSNNWAVLVST 48
M + + L+F + L ++ WAVLV+
Sbjct: 15 MFSDVLASWLVLLLFLSSLHGSVARPNRLEWEPVIRLPGEPVDADVEDEIGTRWAVLVAG 74
Query: 49 SRFWFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDL 108
S + NYRH A+ Y+ + + G+ + I++ + DDIA + N PG + N+ + ++
Sbjct: 75 SNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIAYSEFNPRPGVIINHP-QGPNV 133
Query: 109 YGESIEVDYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLK 167
Y + + DY G VT +N + L DK K + S IFIY + HGG L
Sbjct: 134 Y-DGVPKDYTGDFVTADNLYAVILGDKSKVRGGSGKVINSKAEDRIFIYYSDHGGPGVLG 192
Query: 168 FQDAEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIE 225
+ + A D D + + + Y ++ ++ C++ ++++ I ++ +S E
Sbjct: 193 MPNMPYVYAMDFIDVLKKKHASRGYQQMVIYVEACESGSVFQGIMPKDIDVYVTTASNAE 252
Query: 226 ESSYSHHS--------DMDIGVAVIDRFTYYTLDFLEKI--DRDSKETMDKLFAEYTFEN 275
ESS+ + + + D Y++ ++E +ET+ + FA
Sbjct: 253 ESSWGTYLVPGVYPASPPEYITCLGDL---YSVAWMEDSETHNLKRETLKQQFASVKERT 309
Query: 276 VHSH----PGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQT 331
++++ T+ N+++ L + G VN+ N ++ E NQ
Sbjct: 310 LNNNNYGLGSHVTEYGDTNITDEKLYLYHG----FDPASVNLP-PNNGRLESKMEVVNQR 364
Query: 332 KNII 335
I
Sbjct: 365 DAEI 368
>emb|CAB16318.1| (Z99174) cysteine proteinase precursor [Vicia narbonensis]
Length = 488
Score = 478 bits (1220), Expect = e-134
Identities = 74/363 (20%), Positives = 139/363 (37%), Gaps = 38/363 (10%)
Query: 1 MNRLLSFILPIFLIFSTVLSSDSPN------------DSVNINNSDPSRHSNNWAVLVST 48
M + + L+ S++ S + ++ WAVLV+
Sbjct: 1 MFSDVLASWLVLLLLSSLHGSVAARPNRLEWEPVIRLPGEPVDADVEDEMGTRWAVLVAG 60
Query: 49 SRFWFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDL 108
S + NYRH A+ Y+ + + G+ + I++ + DDIA N N PG + N+ + ++
Sbjct: 61 SNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIAYNEMNPRPGVIINHP-QGPNV 119
Query: 109 YGESIEVDYRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLK 167
Y + + DY G VT ENF + L DK K + S IFIY + HGG L
Sbjct: 120 Y-DGVPKDYNGDFVTAENFYAVILGDKSKVRGGSGKVINSKAEDRIFIYCSDHGGPGVLG 178
Query: 168 FQDAEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIE 225
+ + A D D + + Y ++ ++ C++ +++E I N+ +S +
Sbjct: 179 MPNMPYVYAMDFIDVLKKKHASGGYKKMVIYVEACESGSIFEGIMPKDINVYVTTASNAQ 238
Query: 226 ESSYSHHS-------DMDIGVAVIDRFTYYTLDFLEKI--DRDSKETMDKLFAEYTFENV 276
E+S+ + + + D Y++ ++E +ET+ + +
Sbjct: 239 ENSWGTYCPGVDPAPPPEYITCLGDL---YSVAWMEDSETHNLKRETVKQQYMSVRERTS 295
Query: 277 HSH----PGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTK 332
+ N+++ L + G D N+ + E NQ
Sbjct: 296 NYKNYPLGSHVMQYGDTNITDEKLYLYHG----FDPADGE-PSSNNDILEAKMEVVNQRD 350
Query: 333 NII 335
I
Sbjct: 351 AEI 353
>ref|NP_563825.1| (NM_100749) putative GPI-anchor transamidase (U32517) [Arabidopsis
thaliana]
Length = 388
Score = 471 bits (1201), Expect = e-132
Identities = 170/332 (51%), Positives = 232/332 (69%), Gaps = 10/332 (3%)
Query: 10 PIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVK 69
I + + S + D + H+NNWAVLV TSRFWFNYRHMANTLSLYRTVK
Sbjct: 2 KILTLVMLLCYSFVSS------TGDTTIHTNNWAVLVCTSRFWFNYRHMANTLSLYRTVK 55
Query: 70 RLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMR 129
RLGIPD +IILML+DD+ACN RN +P VFNN + ++LYG+++EVDYRGYEVTVENF+R
Sbjct: 56 RLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLR 115
Query: 130 LLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQ 189
+LT + ++ PRSKRLLSDE S+I +Y+TGHGG+EFLKFQDAEE+ +HDLADA QM ++
Sbjct: 116 VLTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEK 175
Query: 190 KRYNEIFFMIDTCQANTMYEKIHSPNILAVGSSEIEESSYSHHSDMDIGVAVIDRFTYYT 249
+R+ E+ M+DTCQA T++ ++ SP +LA+GSS E+SYSHH D DIGV+V+DRFTYYT
Sbjct: 176 RRFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYT 235
Query: 250 LDFLEKIDRDSKETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFGNVQNVVVD 309
L F E+++ +++ LF Y + S RTDL++ ++ EV +T+FFG+V +
Sbjct: 236 LAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHT 295
Query: 310 DVNM----ELLENEKVTESNEDNNQTKNIIKE 337
D + K+ N +++ +KE
Sbjct: 296 DSAYKAFSSKISERKINSEMPFNQLSEHDLKE 327
>gb|AAF45703.1| (AE003422) EG:133E12.3 gene product [Drosophila melanogaster]
Length = 322
Score = 454 bits (1158), Expect = e-127
Identities = 170/322 (52%), Positives = 226/322 (69%), Gaps = 9/322 (2%)
Query: 7 FILPIFLIFSTVLSS-------DSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMA 59
++ +IF+ +L+S S ++ + S H+NNWAVLV SRFWFNYRH+A
Sbjct: 1 MLVKFVVIFALILASCRVEADNTSVLPEGFVDAAQRSTHTNNWAVLVDASRFWFNYRHVA 60
Query: 60 NTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRG 119
N LS+YR+VKRLGIPDSQIILM++DD+ACN RN PG V+NN ++ I++YG+ +EVDYRG
Sbjct: 61 NVLSIYRSVKRLGIPDSQIILMIADDMACNARNPRPGQVYNNANQHINVYGDDVEVDYRG 120
Query: 120 YEVTVENFMRLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDL 179
YEVTVENF+RLLT + + RSK+LLSD SN+ IYLTGHGG+ FLKFQD+EEI++ +L
Sbjct: 121 YEVTVENFVRLLTGRTQNGTARSKKLLSDAGSNVLIYLTGHGGDGFLKFQDSEEITSQEL 180
Query: 180 ADAFSQMYDQKRYNEIFFMIDTCQANTMYEKIHSPNILAVGSSEIEESSYSHHSDMDIGV 239
AD QM+++KRYNE+FFM+DTCQA ++YEK SPN+LAV SS + E S SHH D IGV
Sbjct: 181 ADGIQQMWEKKRYNELFFMVDTCQAASLYEKFTSPNVLAVASSLVGEDSLSHHVDPSIGV 240
Query: 240 AVIDRFTYYTLDFLEKIDRDSKETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDF 299
+IDR+TYY L+FLEK+ SK T+ + S G+R DL+ R+ +V +TDF
Sbjct: 241 YMIDRYTYYALEFLEKVQPFSKRTIGEFLQVCPKRVCISTVGVRKDLYPRDPHKVPITDF 300
Query: 300 FGNVQNVVVDDV--NMELLENE 319
FG ++ V N+ L E
Sbjct: 301 FGAIRPTRVSTDRINVTLANEE 322
>pir||T13411 hypothetical protein 133E12.3 - fruit fly (Drosophila melanogaster)
emb|CAA15687.1| (AL009192) /prediction=(method:''genefinder'', version:''084'',
score:''46.36'')~/prediction=(method:''genscan'',
version:''1.0'',
score:''114.92'')~/match=(desc:''GPI-ANCHOR TRANSMIDASE
(EC 3.-.-.-)'', species:''SACCHAROMYCES CEREVISIAE
(BAKER'S YEAST>
Length = 326
Score = 454 bits (1157), Expect = e-126
Identities = 170/322 (52%), Positives = 226/322 (69%), Gaps = 9/322 (2%)
Query: 7 FILPIFLIFSTVLSS-------DSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMA 59
++ +IF+ +L+S S ++ + S H+NNWAVLV SRFWFNYRH+A
Sbjct: 5 MLVKFVVIFALILASCRVEADNTSVLPEGFVDAAQRSTHTNNWAVLVDASRFWFNYRHVA 64
Query: 60 NTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRG 119
N LS+YR+VKRLGIPDSQIILM++DD+ACN RN PG V+NN ++ I++YG+ +EVDYRG
Sbjct: 65 NVLSIYRSVKRLGIPDSQIILMIADDMACNARNPRPGQVYNNANQHINVYGDDVEVDYRG 124
Query: 120 YEVTVENFMRLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDL 179
YEVTVENF+RLLT + + RSK+LLSD SN+ IYLTGHGG+ FLKFQD+EEI++ +L
Sbjct: 125 YEVTVENFVRLLTGRTQNGTARSKKLLSDAGSNVLIYLTGHGGDGFLKFQDSEEITSQEL 184
Query: 180 ADAFSQMYDQKRYNEIFFMIDTCQANTMYEKIHSPNILAVGSSEIEESSYSHHSDMDIGV 239
AD QM+++KRYNE+FFM+DTCQA ++YEK SPN+LAV SS + E S SHH D IGV
Sbjct: 185 ADGIQQMWEKKRYNELFFMVDTCQAASLYEKFTSPNVLAVASSLVGEDSLSHHVDPSIGV 244
Query: 240 AVIDRFTYYTLDFLEKIDRDSKETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDF 299
+IDR+TYY L+FLEK+ SK T+ + S G+R DL+ R+ +V +TDF
Sbjct: 245 YMIDRYTYYALEFLEKVQPFSKRTIGEFLQVCPKRVCISTVGVRKDLYPRDPHKVPITDF 304
Query: 300 FGNVQNVVVDDV--NMELLENE 319
FG ++ V N+ L E
Sbjct: 305 FGAIRPTRVSTDRINVTLANEE 326
>emb|CAB42655.1| (AJ238882) putative preprolegumain [Vicia narbonensis]
Length = 380
Score = 452 bits (1151), Expect = e-126
Identities = 60/291 (20%), Positives = 120/291 (40%), Gaps = 24/291 (8%)
Query: 60 NTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRG 119
+ Y+ +++ G+ + II+ + DDIA + N PG + N+ ++Y E + DY G
Sbjct: 1 DVCHAYQLLRKGGLKEENIIVFMYDDIAYSEENPRPGVIINSP-HGENVY-EGVPKDYTG 58
Query: 120 YEVTVENFM-RLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHD 178
+VTV NF LL +K K + S N IF++ + HGG L + + A D
Sbjct: 59 EDVTVGNFFAALLGNKSALSGGSGKVVDSGPNDRIFVFYSDHGGPGVLGMPTSPYMYASD 118
Query: 179 LADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHHS--- 233
L + + Y + F ++ C++ +++E + NI A ++ EESS+ +
Sbjct: 119 LVEVLKIKHAAGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTAANAEESSWGTYCPGE 178
Query: 234 ----DMDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHSH---PGIRT 284
+ + D Y++ ++E D + ET+ + + + +
Sbjct: 179 NPSPPPEYETCLADL---YSVAWMEDSDIHNLQTETLHQQYELVKERTSNGNSNYGSHVM 235
Query: 285 DLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
+S+ L + G+ + N + + ++ NQ +
Sbjct: 236 QYGDIELSKDSLFLYLGS----NPSNENFTFVGRNSLVPPSKAINQRDADL 282
>gb|AAF73260.1|AF154349_1 (AF154349) legumain [Rattus norvegicus]
Length = 435
Score = 445 bits (1133), Expect = e-124
Identities = 89/302 (29%), Positives = 149/302 (48%), Gaps = 18/302 (5%)
Query: 7 FILPIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYR 66
I + ++ S VL +V+I DP +W V+V+ S W+NYRH A+ Y+
Sbjct: 1 MIWKVAVLLSLVLG----AGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQ 56
Query: 67 TVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVEN 126
+ R GIPD QII+M+ DDIA N N PG V N + D+Y + + DY G +VT EN
Sbjct: 57 IIHRNGIPDEQIIVMMYDDIANNEENPTPGVVINRPN-GTDVY-KGVPKDYTGEDVTPEN 114
Query: 127 FMRLL-TDKWDSDQ-PRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFS 184
F+ +L D+ K L S ++F+Y T HG L F + E++ DL
Sbjct: 115 FLAVLRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIR 173
Query: 185 QMYDQKRYNEIFFMIDTCQANTMYEKI-HSPNILAVGSSEIEESSYSHHSDMDIGVAVID 243
MY+ K Y ++ F I+ C++ +M + ++ A ++ ESSY+ + D + + D
Sbjct: 174 YMYEHKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEERSTYLGD 233
Query: 244 RFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFG 301
+Y+++++E D + KET+ K + H++ +++S + + F G
Sbjct: 234 ---WYSVNWMEDSDVEDLTKETLHKQYHLVK---SHTNTSHVMQYGNKSISTMKVMQFQG 287
Query: 302 NV 303
Sbjct: 288 MK 289
>emb|CAB51545.1| (AJ243876) vacuolar processing enzyme [Lycopersicon esculentum]
Length = 460
Score = 444 bits (1132), Expect = e-123
Identities = 81/345 (23%), Positives = 143/345 (40%), Gaps = 32/345 (9%)
Query: 12 FLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVKRL 71
FLI V+ ++ N + WAVLV+ S+ W+NYRH AN Y+ +K+
Sbjct: 10 FLIALFVVLTEGRNVIERFDEDYEDSIGTKWAVLVAGSKEWYNYRHQANLCHAYQLLKKG 69
Query: 72 GIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMRL- 130
G+ D II+ + DDIA NP N PG + NN D+Y + + DY G + +NF +
Sbjct: 70 GLKDEHIIVFMYDDIANNPENPRPGVIINNP-HGHDVY-KGVPKDYTGKDCNAQNFYSVI 127
Query: 131 LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQK 190
L +K K + S N IFIY T HG + + + A DL + + + +
Sbjct: 128 LGNKSALTGGSGKVVNSGPNDYIFIYYTDHGAPGLVGMPEDPPVYAIDLNEVLKKKHASR 187
Query: 191 RYNEIFFMIDTCQANTMYEKIHS--PNILAVGSSEIEESSYSHH------------SDMD 236
Y ++ F ++ C + +M+ + NI A SS+ +E ++ +
Sbjct: 188 TYKKMVFYLEACDSGSMFADLLDEGLNIYATTSSKPDEDGWATYCYFTGDTSCYGECPPK 247
Query: 237 --IGVAVIDRFTYYTLDFLEKIDRDSK--ETMDKLFAEYTFENVH--SHPGIRTDLFKRN 290
+ D F ++ +LE D ET++K + ++ +H +
Sbjct: 248 DFKDNCLGDLF---SVSWLENSDLHDLQVETLEKQYLRIHKRVLNNGTHGSHMVQYGDLH 304
Query: 291 VSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKNII 335
+++ L+ + G+ N ++ NQ +
Sbjct: 305 INKDALSIYMGSN------SPKHTWSANNNNASNSRHVNQRDVQL 343
>ref|NP_071562.1| (NM_022226) protease, cysteine, 1 (legumain) [Rattus norvegicus]
dbj|BAA84750.1| (AB032766) legumain [Rattus norvegicus]
Length = 435
Score = 443 bits (1130), Expect = e-123
Identities = 88/302 (29%), Positives = 148/302 (48%), Gaps = 18/302 (5%)
Query: 7 FILPIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYR 66
+ ++ S VL +V+I DP +W V+V+ S W+NYRH A+ Y+
Sbjct: 1 MTWRVAVLLSLVLG----AGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQ 56
Query: 67 TVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVEN 126
+ R GIPD QII+M+ DDIA N N PG V N + D+Y + + DY G +VT EN
Sbjct: 57 IIHRNGIPDEQIIVMMYDDIANNEENPTPGVVINRPN-GTDVY-KGVPKDYTGEDVTPEN 114
Query: 127 FMRLL-TDKWDSDQ-PRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFS 184
F+ +L D+ K L S ++F+Y T HG L F + E++ DL
Sbjct: 115 FLAVLRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIR 173
Query: 185 QMYDQKRYNEIFFMIDTCQANTMYEKI-HSPNILAVGSSEIEESSYSHHSDMDIGVAVID 243
MY+ K Y ++ F I+ C++ +M + ++ A ++ ESSY+ + D + + D
Sbjct: 174 YMYEHKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEERSTYLGD 233
Query: 244 RFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFG 301
+Y+++++E D + KET+ K + H++ +++S + + F G
Sbjct: 234 ---WYSVNWMEDSDVEDLTKETLHKQYHLVK---SHTNTSHVMQYGNKSISTMKVMQFQG 287
Query: 302 NV 303
Sbjct: 288 MK 289
>ref|NP_035305.1| (NM_011175) protease, cysteine, 1; preprolegumain [Mus musculus]
emb|CAA04439.1| (AJ000990) legumain [Mus musculus]
gb|AAF21659.1|AF044266_1 (AF044266) preprolegumain; cysteine Protease [Mus musculus]
Length = 435
Score = 442 bits (1126), Expect = e-123
Identities = 87/302 (28%), Positives = 150/302 (48%), Gaps = 18/302 (5%)
Query: 7 FILPIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYR 66
+ ++ S VL +V + DP +W V+V+ S W+NYRH A+ Y+
Sbjct: 1 MTWRVAVLLSLVLG----AGAVPVGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQ 56
Query: 67 TVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVEN 126
+ R GIPD QII+M+ DDIA + N PG V N + D+Y + + DY G +VT EN
Sbjct: 57 IIHRNGIPDEQIIVMMYDDIANSEENPTPGVVINRPN-GTDVY-KGVLKDYTGEDVTPEN 114
Query: 127 FMRLLTDKWDS--DQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFS 184
F+ +L ++ + K L S ++FIY T HG L F + +++ DL
Sbjct: 115 FLAVLRGDAEAVKGKGSGKVLKSGPRDHVFIYFTDHGATGILVFPN-DDLHVKDLNKTIR 173
Query: 185 QMYDQKRYNEIFFMIDTCQANTMYEKI-HSPNILAVGSSEIEESSYSHHSDMDIGVAVID 243
MY+ K Y ++ F I+ C++ +M + N+ A ++ +ESSY+ + D + G + D
Sbjct: 174 YMYEHKMYQKMVFYIEACESGSMMNHLPDDINVYATTAANPKESSYACYYDEERGTYLGD 233
Query: 244 RFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFG 301
+Y+++++E D + KET+ K + H++ +++S + + F G
Sbjct: 234 ---WYSVNWMEDSDVEDLTKETLHKQYHLVK---SHTNTSHVMQYGNKSISTMKVMQFQG 287
Query: 302 NV 303
Sbjct: 288 MK 289
>ref|NP_188656.1| (NM_112912) vacuolar processing enzyme/asparaginyl endopeptidase,
putative [Arabidopsis thaliana]
dbj|BAB01880.1| (AP000383) vacuolar processing enzyme (proteinase) [Arabidopsis
thaliana]
Length = 466
Score = 441 bits (1123), Expect = e-122
Identities = 87/343 (25%), Positives = 150/343 (43%), Gaps = 24/343 (6%)
Query: 5 LSFILPIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSL 64
+ L LIFS + ++ +SD S WAVLV+ S ++NYRH A+
Sbjct: 10 ILVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHA 69
Query: 65 YRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTV 124
Y+ +++ G+ D II+ + DDIA + N PG + N D D+Y + + DY V V
Sbjct: 70 YQILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPD-GEDVY-KGVPKDYTKEAVNV 127
Query: 125 ENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAF 183
+NF + L ++ K + S N NIFIY HG + +E+ A D +
Sbjct: 128 QNFYNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVL 187
Query: 184 SQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYS-------HHSD 234
+M+ +K+YN++ ++ C++ +M+E I + NI AV ++ +ESS+
Sbjct: 188 EKMHKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPP 247
Query: 235 MDIGVAVIDRFTYYTLDFLEKIDRDS--KETMDKLFAEYTFEN--VHSHPGIRTDLFKRN 290
+IG + D F ++ +LE D KET+++ +
Sbjct: 248 SEIGTCLGDTF---SISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVPETSHVCRFGTEK 304
Query: 291 VSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNED-NNQTK 332
+ + L+ + G ++ N E+ SN N
Sbjct: 305 MLKDYLSSYIGR----NPENDNFTFTESFSSPISNSGLVNPRD 343
>pir||T00731 hypothetical protein F22O13.26 - Arabidopsis thaliana
Length = 428
Score = 439 bits (1118), Expect = e-122
Identities = 157/336 (46%), Positives = 222/336 (65%), Gaps = 14/336 (4%)
Query: 10 PIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFW----FNYRHMANTLSLY 65
I + + S + D + H+NNWAVLV TSRF + + L +
Sbjct: 2 KILTLVMLLCYSFVSS------TGDTTIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVS 55
Query: 66 RTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVE 125
RTVKRLGIPD +IILML+DD+ACN RN +P VFNN + ++LYG+++EVDYRGYEVTVE
Sbjct: 56 RTVKRLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVE 115
Query: 126 NFMRLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQ 185
NF+R+LT + ++ PRSKRLLSDE S+I +Y+TGHGG+EFLKFQDAEE+ +HDLADA Q
Sbjct: 116 NFLRVLTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQ 175
Query: 186 MYDQKRYNEIFFMIDTCQANTMYEKIHSPNILAVGSSEIEESSYSHHSDMDIGVAVIDRF 245
M +++R+ E+ M+DTCQA T++ ++ SP +LA+GSS E+SYSHH D DIGV+V+DRF
Sbjct: 176 MKEKRRFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRF 235
Query: 246 TYYTLDFLEKIDRDSKETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFGNVQN 305
TYYTL F E+++ +++ LF Y + S RTDL++ ++ EV +T+FFG+V
Sbjct: 236 TYYTLAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVME 295
Query: 306 VVVDDVNM----ELLENEKVTESNEDNNQTKNIIKE 337
+ D + K+ N +++ +KE
Sbjct: 296 TIHTDSAYKAFSSKISERKINSEMPFNQLSEHDLKE 331
>dbj|BAA09530.1| (D55696) cysteine protease [Homo sapiens]
Length = 433
Score = 437 bits (1113), Expect = e-121
Identities = 84/304 (27%), Positives = 144/304 (46%), Gaps = 20/304 (6%)
Query: 7 FILPIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYR 66
+ + + S L + DP +WAV+V+ S W+NYRH A+ Y+
Sbjct: 1 MVWKVAVFLSVALGI------GAVPIDDPEDGGKHWAVIVAGSNGWYNYRHQADACHAYQ 54
Query: 67 TVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVEN 126
+ R GIPD QI++M+ DDIA + N PG V N + D+Y + + DY G +VT +N
Sbjct: 55 IIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPN-GTDVY-QGVPKDYTGEDVTPQN 112
Query: 127 FMRLLTDKWDS--DQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFS 184
F+ +L ++ K L S ++FIY T HG L F + E++ DL +
Sbjct: 113 FLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIH 171
Query: 185 QMYDQKRYNEIFFMIDTCQANTMYEKIHS-PNILAVGSSEIEESSYSHHSDMDIGVAVID 243
MY K Y ++ F I+ C++ +M + N+ A ++ ESSY+ + D + D
Sbjct: 172 YMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD 231
Query: 244 RFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFG 301
+Y+++++E D + KET+ K + H++ + +S + + F G
Sbjct: 232 ---WYSVNWMEDSDVEDLTKETLHKQYHLVK---SHTNTSHVMQYGNKTISTMKVMQFQG 285
Query: 302 NVQN 305
+
Sbjct: 286 MKRK 289
>ref|NP_010618.1| (NC_001136) Protein involved in the attachment of
glycosylphosphatidylinositol (GPI) anchors to proteins;
Gpi8p [Saccharomyces cerevisiae]
sp|P49018|GPI8_YEAST GPI-ANCHOR TRANSAMIDASE
pir||S59796 probable membrane protein YDR331w - yeast (Saccharomyces
cerevisiae)
gb|AAB64766.1| (U32517) Ydr331wp [Saccharomyces cerevisiae]
Length = 411
Score = 436 bits (1112), Expect = e-121
Identities = 221/306 (72%), Positives = 264/306 (86%), Gaps = 1/306 (0%)
Query: 5 LSFILPIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSL 64
++ LP+ L++ +L N++ + + + ++NNWAVLVSTSRFWFNYRHMAN LS+
Sbjct: 3 IAMHLPLLLLYIFLLPLSGANNT-DAAHEVIATNTNNWAVLVSTSRFWFNYRHMANVLSM 61
Query: 65 YRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTV 124
YRTVKRLGIPDSQIILMLSDD+ACN RN FPGSVFNN D AIDLYG+S+EVDYRGYEVTV
Sbjct: 62 YRTVKRLGIPDSQIILMLSDDVACNSRNLFPGSVFNNKDHAIDLYGDSVEVDYRGYEVTV 121
Query: 125 ENFMRLLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFS 184
ENF+RLLTD+W D P+SKRLL+DENSNIFIY+TGHGG++FLKFQDAEEI++ D+ADAF
Sbjct: 122 ENFIRLLTDRWTEDHPKSKRLLTDENSNIFIYMTGHGGDDFLKFQDAEEIASEDIADAFQ 181
Query: 185 QMYDQKRYNEIFFMIDTCQANTMYEKIHSPNILAVGSSEIEESSYSHHSDMDIGVAVIDR 244
QMY++KRYNEIFFMIDTCQANTMY K +SPNILAVGSSE++ESSYSHHSD++IGVAVIDR
Sbjct: 182 QMYEKKRYNEIFFMIDTCQANTMYSKFYSPNILAVGSSEMDESSYSHHSDVEIGVAVIDR 241
Query: 245 FTYYTLDFLEKIDRDSKETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFGNVQ 304
FTYY LDFLE+ID++S T+ LF +TFE +HSH G+RTDLF RN SEVL+TDFF NVQ
Sbjct: 242 FTYYCLDFLEQIDKNSTLTLQDLFDSFTFEKIHSHVGVRTDLFDRNPSEVLITDFFANVQ 301
Query: 305 NVVVDD 310
NV+ DD
Sbjct: 302 NVIPDD 307
>ref|XP_007399.4| (XM_007399) legumain [Homo sapiens]
Length = 401
Score = 435 bits (1109), Expect = e-121
Identities = 83/304 (27%), Positives = 143/304 (46%), Gaps = 20/304 (6%)
Query: 7 FILPIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYR 66
+ + + S L + DP +W V+V+ S W+NYRH A+ Y+
Sbjct: 1 MVWKVAVFLSVALGI------GAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQ 54
Query: 67 TVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVEN 126
+ R GIPD QI++M+ DDIA + N PG V N + D+Y + + DY G +VT +N
Sbjct: 55 IIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPN-GTDVY-QGVPKDYTGEDVTPQN 112
Query: 127 FMRLLTDKWDS--DQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFS 184
F+ +L ++ K L S ++FIY T HG L F + E++ DL +
Sbjct: 113 FLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIH 171
Query: 185 QMYDQKRYNEIFFMIDTCQANTMYEKIHS-PNILAVGSSEIEESSYSHHSDMDIGVAVID 243
MY K Y ++ F I+ C++ +M + N+ A ++ ESSY+ + D + D
Sbjct: 172 YMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD 231
Query: 244 RFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFG 301
+Y+++++E D + KET+ K + H++ + +S + + F G
Sbjct: 232 ---WYSVNWMEDSDVEDLTKETLHKQYHLVK---SHTNTSHVMQYGNKTISTMKVMQFQG 285
Query: 302 NVQN 305
+
Sbjct: 286 MKRK 289
>emb|CAB42651.1| (AJ238881) putative preprolegumain [Nicotiana tabacum]
Length = 419
Score = 435 bits (1109), Expect = e-121
Identities = 63/296 (21%), Positives = 116/296 (38%), Gaps = 29/296 (9%)
Query: 57 HMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVD 116
H A+ Y+ +KR G+ D I++ + DDIA + N PG + N+ + D+Y + D
Sbjct: 1 HQADVCHAYQILKRGGLKDENIVVFMYDDIAKSELNPRPGVIINHPN-GSDVYA-GVPKD 58
Query: 117 YRGYEVTVENFMRL-LTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEIS 175
Y G VT N + L DK K + S N IF+Y + HGG L + +
Sbjct: 59 YTGEHVTAANLYAVLLGDKSAVKGGSGKIVDSKPNDRIFLYYSDHGGPGVLGMPNMPFLY 118
Query: 176 AHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKI--HSPNILAVGSSEIEESSYSHHS 233
A D + + + Y E+ I+ C++ +++E + NI +S EESS+ +
Sbjct: 119 AKDFIEVLKKKHAAGTYKEMVLYIEACESGSVFEGMMPEDLNIYVTTASNAEESSWGTYC 178
Query: 234 D-------MDIGVAVIDRFTYYTLDFLEK--IDRDSKETMDKLFAEYTFENVHSH----P 280
+ + D Y++ ++E KET+ + + + + +
Sbjct: 179 PGMDPPPPPEYITCLGDL---YSVAWMEDSESHNLKKETIKQQYEKVKERTSNFNNYNAG 235
Query: 281 GIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVT-ESNEDNNQTKNII 335
+ + + + + G N+ K+ E NQ +
Sbjct: 236 SHVMEYGSKEIKPEKVYLYQG----FDPATANLS---ANKIAFAHVEVVNQRDADL 284
>gb|AAH03061.1|AAH03061 (BC003061) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 435 bits (1108), Expect = e-121
Identities = 84/304 (27%), Positives = 143/304 (46%), Gaps = 20/304 (6%)
Query: 7 FILPIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYR 66
+ + + S L I DP +W V+V+ S W+NYRH A+ Y+
Sbjct: 1 MVWKVAVFLSVALGI------GAIPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQ 54
Query: 67 TVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVEN 126
+ R GIPD QI++M+ DDIA + N PG V N + D+Y + + DY G +VT +N
Sbjct: 55 IIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPN-GTDVY-QGVPKDYTGEDVTPQN 112
Query: 127 FMRLLTDKWDS--DQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFS 184
F+ +L ++ K L S ++FIY T HG L F + E++ DL +
Sbjct: 113 FLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIH 171
Query: 185 QMYDQKRYNEIFFMIDTCQANTMYEKIHS-PNILAVGSSEIEESSYSHHSDMDIGVAVID 243
MY K Y ++ F I+ C++ +M + N+ A ++ ESSY+ + D + D
Sbjct: 172 YMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD 231
Query: 244 RFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFG 301
+Y+++++E D + KET+ K + H++ + +S + + F G
Sbjct: 232 ---WYSVNWMEDSDVEDLTKETLHKQYHLVK---SHTNTSHVMQYGNKTISTMKVMQFQG 285
Query: 302 NVQN 305
+
Sbjct: 286 MKRK 289
>ref|NP_005597.1| (NM_005606) legumain; protease, cysteine, 1 (legumain) [Homo
sapiens]
sp|Q99538|LEGU_HUMAN Legumain precursor (Asparaginyl endopeptidase)
emb|CAA70989.1| (Y09862) legumain [Homo sapiens]
gb|AAH08004.1|AAH08004 (BC008004) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 435 bits (1108), Expect = e-121
Identities = 83/304 (27%), Positives = 143/304 (46%), Gaps = 20/304 (6%)
Query: 7 FILPIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYR 66
+ + + S L + DP +W V+V+ S W+NYRH A+ Y+
Sbjct: 1 MVWKVAVFLSVALGI------GAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQ 54
Query: 67 TVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVEN 126
+ R GIPD QI++M+ DDIA + N PG V N + D+Y + + DY G +VT +N
Sbjct: 55 IIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPN-GTDVY-QGVPKDYTGEDVTPQN 112
Query: 127 FMRLLTDKWDS--DQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFS 184
F+ +L ++ K L S ++FIY T HG L F + E++ DL +
Sbjct: 113 FLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIH 171
Query: 185 QMYDQKRYNEIFFMIDTCQANTMYEKIHS-PNILAVGSSEIEESSYSHHSDMDIGVAVID 243
MY K Y ++ F I+ C++ +M + N+ A ++ ESSY+ + D + D
Sbjct: 172 YMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD 231
Query: 244 RFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFG 301
+Y+++++E D + KET+ K + H++ + +S + + F G
Sbjct: 232 ---WYSVNWMEDSDVEDLTKETLHKQYHLVK---SHTNTSHVMQYGNKTISTMKVMQFQG 285
Query: 302 NVQN 305
+
Sbjct: 286 MKRK 289
>dbj|BAB69947.1| (AB060129) legumain [Bos taurus]
Length = 433
Score = 431 bits (1099), Expect = e-120
Identities = 87/300 (29%), Positives = 146/300 (48%), Gaps = 20/300 (6%)
Query: 7 FILPIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYR 66
I ++ S VL + + DP +W V+V+ S W+NYRH A+ Y+
Sbjct: 1 MIWEFTVLLSLVLGT------GAVPLEDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQ 54
Query: 67 TVKRLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVEN 126
V R GIPD QII+M+ DDIA + N PG V N + D+Y + + DY G +VT +N
Sbjct: 55 IVHRNGIPDEQIIVMMYDDIANSEDNPTPGIVINRPN-GSDVY-QGVLKDYTGEDVTPKN 112
Query: 127 FMRLLTDKWDS--DQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFS 184
F+ +L ++ K L S ++F+Y T HG L F + E++ DL +
Sbjct: 113 FLAVLRGDAEAVKGVGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNETIR 171
Query: 185 QMYDQKRYNEIFFMIDTCQANTMYEKI-HSPNILAVGSSEIEESSYSHHSDMDIGVAVID 243
MY+ K Y ++ F I+ C++ +M + N+ A ++ ESSY+ + D + D
Sbjct: 172 YMYEHKMYQKMVFYIEACESGSMMNHLPPDINVYATTAANPRESSYACYYDEQRSTFLGD 231
Query: 244 RFTYYTLDFLEKIDRDS--KETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFG 301
+Y+++++E D + KET+ K + H++ +++S + L F G
Sbjct: 232 ---WYSVNWMEDSDVEDLTKETLHKQYQLVK---SHTNTSHVMQYGNKSISAMKLMQFQG 285
>gb|AAF99765.1|AC003981_15 (AC003981) F22O13.24 [Arabidopsis thaliana]
Length = 407
Score = 427 bits (1088), Expect = e-118
Identities = 154/332 (46%), Positives = 216/332 (64%), Gaps = 27/332 (8%)
Query: 10 PIFLIFSTVLSSDSPNDSVNINNSDPSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVK 69
I + + S + D + H+NNWAVLV TSRFW TVK
Sbjct: 2 KILTLVMLLCYSFVSS------TGDTTIHTNNWAVLVCTSRFW--------------TVK 41
Query: 70 RLGIPDSQIILMLSDDIACNPRNAFPGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMR 129
RLGIPD +IILML+DD+ACN RN +P VFNN + ++LYG+++EVDYRGYEVTVENF+R
Sbjct: 42 RLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLR 101
Query: 130 LLTDKWDSDQPRSKRLLSDENSNIFIYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQ 189
+LT + ++ PRSKRLLSDE S+I +Y+TGHGG+EFLKFQDAEE+ +HDLADA QM ++
Sbjct: 102 VLTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEK 161
Query: 190 KRYNEIFFMIDTCQANTMYEKIHSPNILAVGSSEIEESSYSHHSDMDIGVAVIDRFTYYT 249
+R+ E+ M+DTCQA T++ ++ SP +LA+GSS E+SYSHH D DIGV+V+DRFTYYT
Sbjct: 162 RRFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYT 221
Query: 250 LDFLEKIDRDSKETMDKLFAEYTFENVHSHPGIRTDLFKRNVSEVLLTDFFGNVQNVVVD 309
L F E+++ +++ Y + S RTDL++ ++ EV +T+FFG+V +
Sbjct: 222 LAFFERLNIYDNASLN---RSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHT 278
Query: 310 DVNM----ELLENEKVTESNEDNNQTKNIIKE 337
D + K+ N +++ +KE
Sbjct: 279 DSAYKAFSSKISERKINSEMPFNQLSEHDLKE 310
>pir||T40853 probable cysteine proteinase (EC 3.4.22.-) [similarity] - fission
yeast (Schizosaccharomyces pombe)
emb|CAB57844.1| (AL121783) putative gpi-anchor transamidase [Schizosaccharomyces
pombe]
emb|CAC13970.1| (AJ250428) GPI8p transamidase [Schizosaccharomyces pombe]
Length = 380
Score = 426 bits (1084), Expect = e-118
Identities = 182/304 (59%), Positives = 232/304 (75%), Gaps = 9/304 (2%)
Query: 35 PSRHSNNWAVLVSTSRFWFNYRHMANTLSLYRTVKRLGIPDSQIILMLSDDIACNPRNAF 94
S H+NNWAVL+STSRFWFNYRH AN L +YR+VKRLGIPDSQIILM++DD ACN RN F
Sbjct: 20 ESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNLF 79
Query: 95 PGSVFNNMDEAIDLYGESIEVDYRGYEVTVENFMRLLTDKWDSDQPRSKRLLSDENSNIF 154
PG+VF+N D A+DLYGE IE+DY+GYEVTVE F+RLLT++ + P SKRLL++E SNI
Sbjct: 80 PGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPENTPASKRLLTNERSNIL 139
Query: 155 IYLTGHGGNEFLKFQDAEEISAHDLADAFSQMYDQKRYNEIFFMIDTCQANTMYEKIHSP 214
IY+TGHGG+ F+KFQDAEE+S+ DLADA Q++ KRYNEI FM+DTCQAN++Y KI+SP
Sbjct: 140 IYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIYSP 199
Query: 215 NILAVGSSEIEESSYSHHSDMDIGVAVIDRFTYYTLDFLE-KIDRDSKETMDKLFAEYTF 273
N+LA+GSSE+ SSYSHH+D+DIGVAVIDRFT+ L+FLE ++D SK TM L Y
Sbjct: 200 NVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLENRVDSKSKLTMQDLINSYNP 259
Query: 274 ENVHSHPGIRTDLFKRNVSEVLLTDFFGNVQNVVVDDVNMELLENEKVTESNEDNNQTKN 333
+HS PG++ +R+ ++L+TDFFGNV+++ L +EK+ N TK
Sbjct: 260 YEIHSTPGVQPINLRRSPDDILITDFFGNVRDIE--------LHSEKINWMLPGENTTKP 311
Query: 334 IIKE 337
IK
Sbjct: 312 SIKR 315
CPU time: 103.16 user secs. 2.53 sys. secs 105.69 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.315 0.175 0.552
Gapped
Lambda K H
0.270 0.0614 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 280456035
Number of Sequences: 887402
Number of extensions: 17244989
Number of successful extensions: 29725
Number of sequences better than 10.0: 91
Number of HSP's better than 10.0 without gapping: 73
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 29224
Number of HSP's gapped (non-prelim): 95
length of query: 383
length of database: 277,845,442
effective HSP length: 58
effective length of query: 325
effective length of database: 226,376,126
effective search space: 73572240950
effective search space used: 73572240950
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.1 bits)
S2: 74 (32.9 bits)