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GPI Biosynthesis Report< | |
| GPI Biosynthesis Main Page | GPI Site Motif | GPI Site Prediction | Home Page B.E. |
| Molecular Function: | part of the transamidase complex, protease |
|---|---|
| Complex: | GPAA1/GAA1, PIG-K/GPI8, PIG-S/GPI17, PIG-T/GPI16 |
| Cellular Localization: | mainly ER lumen |
| Domain Architecture: | N Signal Peptide-----globular Domain-------|TM (optional)|-- C |
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Orthologues |  |
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| Protein | Organism | Sequence Length | GenBank Entry |
| T00731 | Arabidopsis thaliana | 428 | gi|7485988| |
| AN0871.1 | Aspergillus nidulans | 397 | |
| P49048 | Caenorhabditis elegans | 322 | gi|3123309| |
| orf6.3728.prot | Candida albicans | 383 | |
| AAF45703.1 | Drosophila melanogaster | 322 | gi|7290241| |
| XP_039644.1 | Homo sapiens | 395 | gi|14724559| |
| CAB55340.1 | Leishmania mexicana | 349 | gi|5834624| |
| BAB29018.1 | Mus musculus | 395 | gi|12851374| |
| XP_327545.1 | Neurospora crassa | 401 | gi|32414131| |
| AAAA01008908 | Oryza sativa | 404 | 19933218 |
| AAL29895.1 | Paramecium tetraurelia | 309 | gi|16923219| |
| CAB96076.1 | Plasmodium falciparum | 493 | gi|8919190| |
| NP_010618.1 | Saccharomyces cerevisiae | 411 | gi|6320538| |
| CAC13970.1 | Schizosaccharomyces pombe | 380 | gi|10862894| |
| CAC67556.1 | Trypanosoma brucei | 319 | gi|15485606| |
Fasta-File of the Family-Members
| Alignments PIG-K: |
- MView - Clustal |
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| OMIM: | Entry 605087 |
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Literature | |
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| PubMed: 11598210 | The GPI transamidase complex of Saccharomyces cerevisiae contains Gaa1p, Gpi8p, and Gpi16p. Mol Biol Cell 2001 Oct;12(10):3295-306 |
| PubMed: 11483512 | PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8. EMBO J 2001 Aug 1;20(15):4088-98 |
| PubMed: 11298746 | Endoplasmic reticulum proteins involved in glycosylphosphatidylinositol-anchor attachment: photocrosslinking studies in a cell-free system. Eur J Biochem 2001 Apr;268(8):2290-300 |
| PubMed: 10793132 | Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins. Mol Biol Cell 2000 May;11(5):1523-33 |
| PubMed: 10727241 | Active site determination of Gpi8p, a caspase-related enzyme required for glycosylphosphatidylinositol anchor addition to proteins. Biochemistry 2000 Mar 28;39(12):3461-71 |
| PubMed: 8978684 | Yeast Gpi8p is essential for GPI anchor attachment onto proteins. EMBO J 1996 Dec 2;15(23):6575-83 |
| PubMed: 8755508 | COOH-terminal processing of nascent polypeptides by the glycosylphosphatidylinositol transamidase in the presence of hydrazine is governed by the same parameters as glycosylphosphatidylinositol addition. Proc Natl Acad Sci U S A 1996 Jul 23;93(15):7528-33 |
| PubMed: 7642644 | An active carbonyl formed during glycosylphosphatidylinositol addition to a protein is evidence of catalysis by a transamidase. J Biol Chem 1995 Aug 18;270(33):19576-82 |
| PubMed: 7878018 | Cleavage without anchor addition accompanies the processing of a nascent protein to its glycosylphosphatidylinositol-anchored form. Proc Natl Acad Sci U S A 1995 Feb 28;92(5):1550-4 |
| PubMed: 7651176 | Processing of nascent proteins to glycosylphosphatidylinositol-anchored forms in cell-free systems. Methods Enzymol 1995;250:536-47 |
Author: Birgit.Eisenhaber@nt.imp.univie.ac.at on internet.