Sequences with E-value BETTER than threshold (0.002) 649 0.0
638 0.0
635 0.0
634 0.0
630 e-179
628 e-179
626 e-178
626 e-178
625 e-178
624 e-178
623 e-177
622 e-177
622 e-177
619 e-176
614 e-175
614 e-174
612 e-174
612 e-174
604 e-172
604 e-172
603 e-171
600 e-170
600 e-170
596 e-169
595 e-169
595 e-169
590 e-167
588 e-167
582 e-165
581 e-165
572 e-162
558 e-158
542 e-153
528 e-149
523 e-147
511 e-144
507 e-142
494 e-139
487 e-136
443 e-123
442 e-123
441 e-123
441 e-123
441 e-123
441 e-122
440 e-122
439 e-122
431 e-119
403 e-111
403 e-111
Alignments
>pir||T00731 hypothetical protein F22O13.26 - Arabidopsis thaliana
Length = 428
Score = 649 bits (1657), Expect = 0.0
Identities = 428/428 (100%), Positives = 428/428 (100%)
Query: 1 MKILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKR 60
MKILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKR
Sbjct: 1 MKILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKR 60
Query: 61 LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 120
LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV
Sbjct: 61 LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 120
Query: 121 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR
Sbjct: 121 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
Query: 181 RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL 240
RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL
Sbjct: 181 RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL 240
Query: 241 AFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD 300
AFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD
Sbjct: 241 AFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD 300
Query: 301 SAYKAFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFG 360
SAYKAFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFG
Sbjct: 301 SAYKAFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFG 360
Query: 361 LSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTNRIEIPCYVVVAEAKMPIFTNAGR 420
LSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTNRIEIPCYVVVAEAKMPIFTNAGR
Sbjct: 361 LSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTNRIEIPCYVVVAEAKMPIFTNAGR 420
Query: 421 PPRESGEA 428
PPRESGEA
Sbjct: 421 PPRESGEA 428
>dbj|BAA76744.1| (D89971) asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
Length = 483
Score = 638 bits (1629), Expect = 0.0
Identities = 80/422 (18%), Positives = 159/422 (36%), Gaps = 44/422 (10%)
Query: 3 ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKR 60
IL + F + D WAVL+ S + H + + LL +
Sbjct: 24 ILRMPSEASRFFQAPATDENDEGTRWAVLIAGSNGYWNYRHQSDVCHAYQLLT------K 77
Query: 61 LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 120
G+ +E I++ + DD+A N N P + N+ H ++Y V DY G +VTV NF
Sbjct: 78 GGLKEENIVVFMYDDIAFNEENPRPGVIINSP-HGNDVY-KGVPKDYVGEDVTVNNFFAA 135
Query: 121 LTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEK 179
+ G S +++ S HI +Y + HGG L + + + DL + +K+
Sbjct: 136 ILGNKSALTGGSGKVVNSGPNDHIFIYYSDHGGPGVLGMPTSPYMYASDLIEVLKKKHAS 195
Query: 180 RRFKELMIMVDTCQAATLFNQLQS--PGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT- 236
+K L ++ C++ ++F L + A ++ E+S+ + D + T
Sbjct: 196 GTYKSLAFYLEGCESGSIFGGLLPEGLNIYATTAANAEESSWGTYCPGDNPSPPPEYETC 255
Query: 237 ---YYTLAFFERLNIYDNASLNSLFRSYDPRLLMS---TAYYRTDLYQPHLVEVPVTNFF 290
Y++A+ E +I N +L + ++ + + Y + + Q V + N
Sbjct: 256 LGDLYSVAWMEDSDI-HNLRTETLHQQFELVKQRTMNGNSAYGSHVMQYGDVGLSKNNVS 314
Query: 291 GSVMETIHTD-------SAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELEN 335
+ D ++ S +++R + + P + ++++
Sbjct: 315 LYLGTNPANDNFPFREKNSLVPPSKAVNQRDADLVHFWDKFPKAPLGSSRKSVAQKQILE 374
Query: 336 TNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDS 395
+ VT+ G FG+ ++ R ++DD W L+ +
Sbjct: 375 AMSHRMHIDDSVTLI----GKLLFGIEEGPELLSSVRPAGQPLVDD----WDCLKTLVRT 426
Query: 396 TN 397
Sbjct: 427 FE 428
>emb|CAC18099.1| (AJ251453) putative legumain [Zea mays]
Length = 486
Score = 635 bits (1623), Expect = 0.0
Identities = 85/422 (20%), Positives = 164/422 (38%), Gaps = 47/422 (11%)
Query: 4 LTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRF--CSLHSLVLTFIFSLLGVSRTVKRL 61
+ L + + D WAVL+ S H + + ++ K+
Sbjct: 29 IRLPSQ--RAAAADETDDGDVGTRWAVLIAGSSGYYNYRHQADICHAYQIM------KKG 80
Query: 62 GIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVL 121
G+ DE II+ + DD+A + N P + N+ ++Y V DY G +V V+NF VL
Sbjct: 81 GLKDENIIVFMYDDIAHSPENPRPGVIINHPQGG-DVYA-GVPKDYTGRDVNVDNFFAVL 138
Query: 122 TGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
G S +++ S HI ++ + HGG L L DL D +K+
Sbjct: 139 LGNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAG 198
Query: 181 RFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT-- 236
+K L+ ++ C++ ++F L V A +S E+S+ + + + T
Sbjct: 199 TYKSLVFYLEACESGSIFEGLLPNDINVYATTASNADESSWGTYCPGEFPSPPPEYDTCL 258
Query: 237 --YYTLAFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFF 290
Y++A+ E + + N SL + Y D + T Y + + Q +E+ V F
Sbjct: 259 GDLYSVAWMEDSD-FHNLRTESLKQQYKLVKDRTAVHDTFSYGSHVMQYGALELNVQRLF 317
Query: 291 GSVMETIHT-------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELEN 335
+ D++ +FS +++R + ++ + ++++ ++EL
Sbjct: 318 SYIGTDPANDGNTFIEDNSLPSFSKAVNQRDADLVYFWQKYRKLADSSHAKNEARKELLE 377
Query: 336 TNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDS 395
+ + V + G FG R + R ++DD W ++ +
Sbjct: 378 VMAHRSHVDSSVELI----GSLLFGSEDGPRVLKAVRAPGEPLVDD----WSCLKSIVRT 429
Query: 396 TN 397
Sbjct: 430 FE 431
>emb|CAB64544.1| (AJ131718) legumain-like protease [Zea mays]
Length = 486
Score = 634 bits (1620), Expect = 0.0
Identities = 85/422 (20%), Positives = 163/422 (38%), Gaps = 47/422 (11%)
Query: 4 LTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRF--CSLHSLVLTFIFSLLGVSRTVKRL 61
+ L + + D WAVL+ S H + + ++ K+
Sbjct: 29 IRLPSQ--RAAAADETDDGAVGTRWAVLIAGSSGYYNYRHQADICHAYQIM------KKG 80
Query: 62 GIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVL 121
G+ DE II+ + DD+A + N P + N+ ++Y V DY G +V V+NF VL
Sbjct: 81 GLKDENIIVFMYDDIAHSPENPRPGVIINHPQGG-DVYA-GVPKDYTGRDVNVDNFFAVL 138
Query: 122 TGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
G S +++ S HI ++ + HGG L L DL D +K+
Sbjct: 139 LGNKTALRGGSGKVVDSGPDDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAG 198
Query: 181 RFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT-- 236
+K L+ ++ C++ ++F L V A +S E+S+ + + + T
Sbjct: 199 TYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCL 258
Query: 237 --YYTLAFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFF 290
Y++A+ E + + N SL + Y D + T Y + + Q +E+ V + F
Sbjct: 259 GDLYSVAWMEDSD-FHNLRTESLKQQYKLVKDRTAVHDTFSYGSHVMQYGALELNVQHLF 317
Query: 291 GSVMETIHT-------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELEN 335
+ D++ +FS +++R + + + ++ + ++EL
Sbjct: 318 SYIGTDPANDGNTFIEDNSLPSFSKAVNQRDADLVYFWQKYRKFADSPPAKSEARKELLE 377
Query: 336 TNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDS 395
+ + V + G FG R + R ++DD W ++ +
Sbjct: 378 VMAHRSHVDSSVELI----GSLLFGSEDGPRVLKAVRAPGEPLVDD----WSCLKSIVRT 429
Query: 396 TN 397
Sbjct: 430 FE 431
>pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17078.1| (Z99956) asparagine-specific endopeptidase precursor [Phaseolus
vulgaris]
Length = 484
Score = 630 bits (1608), Expect = e-179
Identities = 79/419 (18%), Positives = 157/419 (36%), Gaps = 44/419 (10%)
Query: 6 LVMLLCYSFVSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGI 63
LV S +H WA+L S + H + + LL ++ G+
Sbjct: 28 LVGDFLRLPSDSGNGDNVHGTRWAILFAGSSGYWNYRHQADICHAYQLL------RKGGL 81
Query: 64 PDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTG 123
DE II+ + DD+A N+ N + N+ N +Y V DY G +VT NF L G
Sbjct: 82 KDENIIVFMYDDIAFNSENPRRGVIINSPN-GDEVY-KGVPKDYTGEDVTAHNFYAALLG 139
Query: 124 RHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRF 182
S +++ S HI ++ + HGG L + + DL + +K+ +
Sbjct: 140 DKSKLTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYASDLNEVLKKKHASGTY 199
Query: 183 KELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT---- 236
K L+ ++ C++ ++F L V A +S E+S+ + + + T
Sbjct: 200 KNLVFYLEACESGSIFEGLLPEDINVYATTASNADESSWGTYCPGEDPSPPPEYSTCLGD 259
Query: 237 YYTLAFFERLNIYDNASLNSLFRSYDPRLLMS---TAYYRTDLYQPHLVEVPVTNFFGSV 293
Y++A+ E + N +L + Y + YY + + Q V + F +
Sbjct: 260 LYSVAWMEDSD-RHNLRTETLHQQYKLVKERTISGGLYYGSHVMQYGDVGLSKDILFHYL 318
Query: 294 METIHT-------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNI 338
+++ + S +++R + + P +++ ++++
Sbjct: 319 GTDPANENLTFVDENSLWSSSKAVNQRDADLVHFWDKFRKAPEGSPKKNEARKQVLEVMS 378
Query: 339 PNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
+ V + G FG+ +N R +++DD W ++ +
Sbjct: 379 HRMHIDDSVELV----GKLLFGIEKAPELLNAVRPAGSALVDD----WDCLKTMVRTFE 429
>gb|AAK15049.1|AF238384_1 (AF238384) asparaginyl endopeptidase [Vigna radiata]
Length = 483
Score = 628 bits (1605), Expect = e-179
Identities = 78/418 (18%), Positives = 156/418 (36%), Gaps = 43/418 (10%)
Query: 6 LVMLLCYSFVSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGI 63
LV S D + WA+L S + H + + +L ++ G+
Sbjct: 28 LVGDFLRLPSDSGNDDNVKGTRWAILFAGSNGYWNYRHQADICHAYQIL------RKGGL 81
Query: 64 PDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTG 123
+E II+ + DD+A N N P + N + ++Y + V DY G + T NF L G
Sbjct: 82 KEENIIVFMYDDIAFNWDNPRPGVIINKPD-GDDVY-EGVPKDYTGEDATAHNFYSALLG 139
Query: 124 RHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRF 182
S +++ S I ++ + HGG L + + DL + +K+ +
Sbjct: 140 DKSALTGGSGKVVNSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTY 199
Query: 183 KELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT---- 236
K L+ ++ C+A ++F L + A +S E+S+ + + + T
Sbjct: 200 KNLVFYLEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGD 259
Query: 237 YYTLAFFERLNIYDNASLNSLFRSYDPRLLM--STAYYRTDLYQPHLVEVPVTNFFGSVM 294
Y++A+ E + N SL + Y S +Y + + Q VE F +
Sbjct: 260 LYSVAWMEDSD-RHNLRTESLHQQYKVVKDRTLSGGWYGSHVMQYGDVEFSKDTLFLYLG 318
Query: 295 ETIHTD-------SAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIP 339
D ++ + S+ +++R + + P +++ ++++
Sbjct: 319 TDPANDNLTFVDENSLWSSSTAVNQRDADLVHFWHKFRKAPEGSPKKNEARKQVLEVMSH 378
Query: 340 NDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
+ V + G FG +N R +++DD W ++ +
Sbjct: 379 RMHIDDSVKLV----GKLLFGFEKAPEVLNAVRPAGSALVDD----WACLKTMVRTFE 428
>emb|CAB64545.1| (AJ131719) legumain-like protease [Zea mays]
Length = 481
Score = 626 bits (1600), Expect = e-178
Identities = 81/415 (19%), Positives = 156/415 (37%), Gaps = 45/415 (10%)
Query: 11 CYSFVSSTGDTTIHTNNWAVLVCTSRF--CSLHSLVLTFIFSLLGVSRTVKRLGIPDERI 68
WAVL+ S H + + ++ K+ G+ DE I
Sbjct: 29 IRLPSDRAAADDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIM------KKGGLKDENI 82
Query: 69 ILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENA 128
++ + DD+A + N P + N+ ++Y V DY G +V V+NF VL G
Sbjct: 83 VVFMYDDIAHSPENPRPGVIINHPQGG-DVYA-GVPKDYTGRDVNVDNFFAVLLGNKTAL 140
Query: 129 VPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMI 187
S +++ S HI ++ + HGG L L DL D +K+ +K L+
Sbjct: 141 RGGSGKVVDSGPNDHISVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVF 200
Query: 188 MVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----YYTLA 241
++ C++ ++F L V A +S E+S+ + + + T Y++A
Sbjct: 201 YLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGDLYSVA 260
Query: 242 FFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETI 297
+ E + + N SL + Y D + T Y + + Q + + V + F +
Sbjct: 261 WMEDSD-FHNLRTESLKQQYNLVKDRTAVQDTFSYGSHVMQYGSLGLNVKHLFSYIGTNP 319
Query: 298 HT-------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIPNDE 342
D++ +FS +++R + ++ + +++ + EL
Sbjct: 320 ANDDNTFIEDNSLPSFSKAVNQRDADLVYFWQKYRKLADSSPEKNEARRELLEVMAHRSH 379
Query: 343 LIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
+ + V + G FG R + R ++DD W + +
Sbjct: 380 VDSSVELI----GSLLFGSEDGPRVLKAVRAAGEPLVDD----WSCLKSTVRTFE 426
>dbj|BAA76745.1| (D89972) asparaginyl endopeptidase (VmPE-1A) [Vigna mungo]
Length = 482
Score = 626 bits (1598), Expect = e-178
Identities = 77/417 (18%), Positives = 155/417 (36%), Gaps = 43/417 (10%)
Query: 7 VMLLCYSFVSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIP 64
V S D + WA+L S + H + + +L ++ G+
Sbjct: 28 VGDFLRLPSDSGNDDNVQGTRWAILFAGSNGYWNYRHQADICHAYQIL------RKGGLK 81
Query: 65 DERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGR 124
+E II+ + DD+A N N P + N + ++Y + V DY G + T NF L G
Sbjct: 82 EENIIVFMYDDIAFNWDNPRPGVIINKPD-GDDVY-EGVPKDYTGEDATAHNFYSALLGD 139
Query: 125 HENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFK 183
S +++ S I ++ + HGG L + + DL + +K+ +K
Sbjct: 140 KSALTGGSGKVVSSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYK 199
Query: 184 ELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----Y 237
L+ ++ C+A ++F L + A +S E+S+ + + + T
Sbjct: 200 NLVFYLEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGDL 259
Query: 238 YTLAFFERLNIYDNASLNSLFRSYDPRLLM--STAYYRTDLYQPHLVEVPVTNFFGSVME 295
Y++A+ E + N SL + Y S +Y + + Q VE F +
Sbjct: 260 YSVAWMEDSD-RHNLRTESLHQQYKVVKDRTLSGGWYGSHVMQYGDVEFSKDALFLYLGT 318
Query: 296 TIHTD-------SAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIPN 340
D ++ + S+ +++R + + P +++ ++++
Sbjct: 319 DPANDNLTFVDENSLWSSSTAVNQRDADLVHFWHKFRKAPEGSPKKNEARKQVLEVMSHR 378
Query: 341 DELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
+ V + G FG +N R +++DD W ++ +
Sbjct: 379 MHIDDSVKLV----GKLLFGFEKAPEVLNAVRPAGSALVDD----WACLKTMVRTFE 427
>sp|P49043|VPE_CITSI VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||S51117 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - sweet
orange
emb|CAA87720.1| (Z47793) cystein proteinase (by similarity) [Citrus sinensis]
prf||2208463A vascular processing protease [Citrus sinensis]
Length = 494
Score = 625 bits (1597), Expect = e-178
Identities = 85/427 (19%), Positives = 164/427 (37%), Gaps = 49/427 (11%)
Query: 3 ILTLVMLLCYSF------VSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGV 54
IL L F D WAVL+ S + H + + LL
Sbjct: 30 ILKLPSEAYRFFHNGGGGAKVNDDDDSVGTRWAVLLAGSNGFWNYRHQADICHAYQLL-- 87
Query: 55 SRTVKRLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTV 114
++ G+ DE II+ + DD+A N N P + N+ H ++Y V DY G +VTV
Sbjct: 88 ----RKGGLKDENIIVFMYDDIAFNEENPRPGVIINHP-HGDDVY-KGVPKDYTGEDVTV 141
Query: 115 ENFLRVLTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAV 173
E F V+ G S +++ S HI ++ + HGG L + + + +L D +
Sbjct: 142 EKFFAVVLGNKTALTGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSRYIYADELIDVL 201
Query: 174 KQMKEKRRFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSV 231
K+ +K L+ ++ C++ ++F L + A +S E+S+ + +I
Sbjct: 202 KKKHASGNYKSLVFYLEACESGSIFEGLLLEGLNIYATTASNAEESSWGTYCPGEIPGPP 261
Query: 232 VDRFT----YYTLAFFERLNIYDNASLNSLFRSYDPRLLMSTAY--YRTDLYQPHLVEVP 285
+ T Y++A+ E +I N +L + Y+ + +Y Y + + Q + +
Sbjct: 262 PEYSTCLGDLYSIAWMEDSDI-HNLRTETLHQQYELVKTRTASYNSYGSHVMQYGDIGLS 320
Query: 286 VTNFFGSVMETIHTD-------SAYKAFSSKISERKIN--------SEMPFNQLSEHDLK 330
N F + D ++ + S +++R + + P + + +
Sbjct: 321 KNNLFTYLGTNPANDNYTFVDENSLRPASKAVNQRDADLLHFWDKYRKAPEGTPRKAEAQ 380
Query: 331 EELENTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFV 390
++ + + + G FG+ +N R ++DD W
Sbjct: 381 KQFFEAMSHRMHVDHSIKLI----GKLLFGIEKGPEILNTVRPAGQPLVDD----WGCLK 432
Query: 391 LLLDSTN 397
L+ +
Sbjct: 433 SLVRTFE 439
>gb|AAF69014.1|AF260827_1 (AF260827) cysteine protease [Ipomoea batatas]
Length = 492
Score = 624 bits (1594), Expect = e-178
Identities = 79/429 (18%), Positives = 154/429 (35%), Gaps = 52/429 (12%)
Query: 4 LTLVMLLCYSFV------SSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVS 55
L L F WAVL+ S + H + + +L
Sbjct: 26 LKLPSEARRFFRPAEEENREADGDDSVGTRWAVLIAGSNGYWNYRHQADICHAYQIL--- 82
Query: 56 RTVKRLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVE 115
K G+ DE I++ + DD+A N N + N+ H ++Y V DY G +VT
Sbjct: 83 ---KAGGLKDENIVVFMYDDIAYNEENPRKGIIINSP-HGEDVYH-GVPKDYTGDDVTAN 137
Query: 116 NFLRVLTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVK 174
N L V+ G S +++ S HI +Y + HGG L + L + +L A+K
Sbjct: 138 NLLAVILGDKSAVKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPYLYADELNAALK 197
Query: 175 QMKEKRRFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVV 232
+ +K L+ ++ C++ ++F + + A +S E+S+ + +
Sbjct: 198 KKHAAGAYKSLVFYLEACESGSIFEGILPKDINIYATTASNAIESSWGTYCPGEYPSPPP 257
Query: 233 DRFT----YYTLAFFERLNIYDNASLNSLFRSYDPRLLMS---TAYYRTDLYQPHLVEVP 285
+ T Y++A+ E +I N SL + Y+ + Y + + Q +E+
Sbjct: 258 EYETCLGDLYSIAWMEDSDI-HNLRTESLKQQYNLVKDRTLNGNTAYGSHVMQYGDLELN 316
Query: 286 VTNFFGSVMETIHTDS---------AYKAFSSKISERKIN--------SEMPFNQLSEHD 328
+ F + ++ A +++R + P + +
Sbjct: 317 ADSLFMYMGTNPANENFTFVDEKSLKLSAPRRAVNQRDADLLHFWDKFRNAPEGSARKSE 376
Query: 329 LKEELENTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLV 388
+++ L + + G FG+ ++ R L ++DD W
Sbjct: 377 AQKQFTEAITHRTHLDNSIALV----GKLLFGMEKGPEVLSSVRATGLPLVDD----WSC 428
Query: 389 FVLLLDSTN 397
+ +
Sbjct: 429 LKSYVRAFE 437
>gb|AAD04883.1| (AF082347) C13 endopeptidase NP1 precursor [Zea mays]
Length = 485
Score = 623 bits (1592), Expect = e-177
Identities = 83/422 (19%), Positives = 160/422 (37%), Gaps = 48/422 (11%)
Query: 4 LTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRF--CSLHSLVLTFIFSLLGVSRTVKRL 61
+ L + D WAVL+ S H + + ++ K+
Sbjct: 29 IRLPSER---AAADETDDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIM------KKG 79
Query: 62 GIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVL 121
G+ DE I++ + DD+A + N P + N+ ++Y V DY G +V V+NF VL
Sbjct: 80 GLKDENIVVFMYDDIAHSPENPRPGVIINHPQGG-DVYA-GVPKDYTGRDVNVDNFFAVL 137
Query: 122 TGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
G S +++ S HI ++ + HGG L L DL D +K+
Sbjct: 138 LGNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAG 197
Query: 181 RFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT-- 236
+K L+ ++ C++ ++F L V A +S E+S+ + + + T
Sbjct: 198 TYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCL 257
Query: 237 --YYTLAFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFF 290
Y++A+ E + + N SL + Y D + T Y + + Q +E+ V + F
Sbjct: 258 GDLYSVAWMEDSD-FHNLRTESLKQQYNLVKDRTAVQDTFSYGSHVMQYGSLELNVKHLF 316
Query: 291 GSVMETIHT-------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELEN 335
+ D++ + S +++R + ++ + +++ + EL
Sbjct: 317 SYIGTNPANDDNTFIEDNSLPSLSKAVNQRDADLVYFWQKYRKLADSSPEKNEARRELLE 376
Query: 336 TNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDS 395
+ + V + G FG R + R ++DD W + +
Sbjct: 377 VMAHRSHVDSSVELI----GSLLFGSEDGPRVLKAVRAAGEPLVDD----WSCLKSTVRT 428
Query: 396 TN 397
Sbjct: 429 FE 430
>dbj|BAA84650.1| (AB025310) asparaginyl endopeptidase [Oryza sativa]
dbj|BAB85400.1| (AP003924) asparaginyl endopeptidase [Oryza sativa (japonica
cultivar-group)]
Length = 501
Score = 622 bits (1589), Expect = e-177
Identities = 85/424 (20%), Positives = 161/424 (37%), Gaps = 47/424 (11%)
Query: 4 LTLVML--LCYSFVSSTGDTTIHTNNWAVLVCTSRF--CSLHSLVLTFIFSLLGVSRTVK 59
L L + + WAVL+ S H + + ++ K
Sbjct: 40 LRLPSERAVAAGAAADDAAEAAEGTRWAVLIAGSNGYYNYRHQADVCHAYQIM------K 93
Query: 60 RLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLR 119
R G+ DE II+ + DD+A N N P + N+ ++Y V DY G EV V+N
Sbjct: 94 RGGLKDENIIVFMYDDIAHNPENPRPGVIINHPQGG-DVYA-GVPKDYTGKEVNVKNLFA 151
Query: 120 VLTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKE 178
VL G S ++L S HI ++ + HGG L L DL D +K+
Sbjct: 152 VLLGNKTAVKGGSGKVLDSGPNDHIFIFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHA 211
Query: 179 KRRFKELMIMVDTCQAATLFNQLQS--PGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT 236
+K L+ ++ C++ ++F L V A +S E+S+ + + + T
Sbjct: 212 AGTYKSLVFYLEACESGSIFEGLLPNGINVYATTASNADESSWGTYCPGEYPSPPPEYDT 271
Query: 237 ----YYTLAFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTN 288
Y++A+ E ++ N SL + Y + + T Y + + + +E+ +
Sbjct: 272 CLGDLYSVAWMEDSDV-HNLRTESLKQQYNLVKERTSVQHTYYSGSHVMEYGSLELNAHH 330
Query: 289 FFGSVMETIHT-------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEEL 333
F + D++ +FS +++R + ++P + +++ +++L
Sbjct: 331 VFMYMGSNPANDNATFVEDNSLPSFSRAVNQRDADLVYFWQKYRKLPESSPEKNEARKQL 390
Query: 334 ENTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLL 393
+ V + G FG R + R ++DD W ++
Sbjct: 391 LEMMAHRSHVDNSVELI----GNLLFGSEEGPRVLKAVRATGEPLVDD----WSCLKSMV 442
Query: 394 DSTN 397
+
Sbjct: 443 RTFE 446
>emb|CAC18100.1| (AJ251454) putative legumain [Zea mays]
Length = 485
Score = 622 bits (1588), Expect = e-177
Identities = 85/422 (20%), Positives = 161/422 (38%), Gaps = 48/422 (11%)
Query: 4 LTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRF--CSLHSLVLTFIFSLLGVSRTVKRL 61
+ L + D WAVL+ S H + + ++ K+
Sbjct: 29 IRLPSER---AAADETDDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIM------KKG 79
Query: 62 GIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVL 121
G+ DE I++ + DD+A + N P + N+ ++Y V DY G EV V+NF VL
Sbjct: 80 GLKDENIVVFMYDDIAHSPENPRPGVIINHPQGG-DVYA-GVPKDYTGREVNVDNFFAVL 137
Query: 122 TGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
G S +++ S HI ++ + HGG L L DL D +K+
Sbjct: 138 LGNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAG 197
Query: 181 RFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT-- 236
+K L+ ++ C++ ++F L V A +S E+S+ + + + T
Sbjct: 198 TYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCL 257
Query: 237 --YYTLAFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFF 290
Y++A+ E + + N SL + Y D + T Y + + Q +E+ V + F
Sbjct: 258 GDLYSVAWMEDSD-FHNLRTESLKQQYNLVKDRTAVQDTFSYGSHVMQYGSLELNVKHLF 316
Query: 291 GSVMETIHT-------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELEN 335
+ D++ +FS +++R + ++ + +++ + EL
Sbjct: 317 SYIGTNPANDDNTSIEDNSLPSFSKAVNQRDADLVYFWQKYRKLADSSHEKNEARRELLE 376
Query: 336 TNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDS 395
+ + V + G FG R + R ++DD W + +
Sbjct: 377 VMAHRSHVDSSVELI----GSLLFGSEDGPRVLKAVRAAGEPLVDD----WSCLKSTVRT 428
Query: 396 TN 397
Sbjct: 429 FE 430
>gb|AAF99765.1|AC003981_15 (AC003981) F22O13.24 [Arabidopsis thaliana]
Length = 407
Score = 619 bits (1582), Expect = e-176
Identities = 405/428 (94%), Positives = 405/428 (94%), Gaps = 21/428 (4%)
Query: 1 MKILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKR 60
MKILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRF TVKR
Sbjct: 1 MKILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFW------------------TVKR 42
Query: 61 LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 120
LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV
Sbjct: 43 LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 102
Query: 121 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR
Sbjct: 103 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 162
Query: 181 RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL 240
RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL
Sbjct: 163 RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL 222
Query: 241 AFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD 300
AFFERLNIYDNA SL RSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD
Sbjct: 223 AFFERLNIYDNA---SLNRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD 279
Query: 301 SAYKAFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFG 360
SAYKAFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFG
Sbjct: 280 SAYKAFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFG 339
Query: 361 LSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTNRIEIPCYVVVAEAKMPIFTNAGR 420
LSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTNRIEIPCYVVVAEAKMPIFTNAGR
Sbjct: 340 LSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTNRIEIPCYVVVAEAKMPIFTNAGR 399
Query: 421 PPRESGEA 428
PPRESGEA
Sbjct: 400 PPRESGEA 407
>ref|NP_195020.1| (NM_119448) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
pir||T05302 vacuolar processing enzyme (EC 3.4.22.-) isozyme gamma precursor -
Arabidopsis thaliana
emb|CAA21203.1| (AL031804) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
emb|CAB80011.1| (AL161582) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
gb|AAK43975.1|AF370160_1 (AF370160) putative gamma-VPE protein [Arabidopsis thaliana]
gb|AAL11612.1|AF424619_1 (AF424619) AT4g32940/F26P21_60 [Arabidopsis thaliana]
gb|AAL15210.1| (AY059104) putative gamma-VPE protein [Arabidopsis thaliana]
Length = 494
Score = 614 bits (1568), Expect = e-175
Identities = 82/427 (19%), Positives = 162/427 (37%), Gaps = 51/427 (11%)
Query: 3 ILTLVMLLCYSFV-SSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVK 59
++ L F + D + WAVLV S + H + + LL +
Sbjct: 34 VIKLPSQASRFFRPAENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLL------R 87
Query: 60 RLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLR 119
+ G+ +E I++ + DD+A N N P + N+ H ++Y V DY G +V V+N
Sbjct: 88 KGGLKEENIVVFMYDDIANNYENPRPGTIINSP-HGKDVY-QGVPKDYTGDDVNVDNLFA 145
Query: 120 VLTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKE 178
V+ G S +++ S HI ++ + HGG L + L ++DL D +K+
Sbjct: 146 VILGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHA 205
Query: 179 KRRFKELMIMVDTCQAATLFNQLQS--PGVLAIGSSLKGENSYSHHL-------DSDIGV 229
+K L+ ++ C++ ++F L + A +S E+S+ + +
Sbjct: 206 LGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYET 265
Query: 230 SVVDRFTYYTLAFFERLNIYDNASLNSLFRSYDPRLLMS---TAYYRTDLYQPHLVEVPV 286
+ D Y++A+ E + N +L + Y+ + Y + + Q V +
Sbjct: 266 CLGD---LYSVAWMEDSGM-HNLQTETLHQQYELVKRRTAPVGYSYGSHVMQYGDVGISK 321
Query: 287 TNFFGSVMETIHTD-------SAYKAFSSKISERKIN--------SEMPFNQLSEHDLKE 331
N + D ++ K S ++R + + P + + ++
Sbjct: 322 DNLDLYMGTNPANDNFTFADANSLKPPSRVTNQRDADLVHFWEKYRKAPEGSARKTEAQK 381
Query: 332 ELENTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVL 391
++ + V + G FG+S +N R ++DD W
Sbjct: 382 QVLEAMSHRLHIDNSVILV----GKILFGISRGPEVLNKVRSAGQPLVDD----WNCLKN 433
Query: 392 LLDSTNR 398
+ + R
Sbjct: 434 QVRAFER 440
>sp|Q39119|VPEG_ARATH VACUOLAR PROCESSING ENZYME, GAMMA-ISOZYME PRECURSOR (GAMMA-VPE)
dbj|BAA18924.1| (D61395) gamma-VPE [Arabidopsis thaliana]
Length = 490
Score = 614 bits (1567), Expect = e-174
Identities = 82/427 (19%), Positives = 162/427 (37%), Gaps = 51/427 (11%)
Query: 3 ILTLVMLLCYSFV-SSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVK 59
++ L F + D + WAVLV S + H + + LL +
Sbjct: 30 VIKLPSQASRFFRPAENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLL------R 83
Query: 60 RLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLR 119
+ G+ +E I++ + DD+A N N P + N+ H ++Y V DY G +V V+N
Sbjct: 84 KGGLKEENIVVFMYDDIANNYENPRPGTIINSP-HGKDVY-QGVPKDYTGDDVNVDNLFA 141
Query: 120 VLTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKE 178
V+ G S +++ S HI ++ + HGG L + L ++DL D +K+
Sbjct: 142 VILGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHA 201
Query: 179 KRRFKELMIMVDTCQAATLFNQLQS--PGVLAIGSSLKGENSYSHHL-------DSDIGV 229
+K L+ ++ C++ ++F L + A +S E+S+ + +
Sbjct: 202 LGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYET 261
Query: 230 SVVDRFTYYTLAFFERLNIYDNASLNSLFRSYDPRLLMS---TAYYRTDLYQPHLVEVPV 286
+ D Y++A+ E + N +L + Y+ + Y + + Q V +
Sbjct: 262 CLGD---LYSVAWMEDSGM-HNLQTETLHQQYELVKRRTAPVGYSYGSHVMQYGDVGISK 317
Query: 287 TNFFGSVMETIHTD-------SAYKAFSSKISERKIN--------SEMPFNQLSEHDLKE 331
N + D ++ K S ++R + + P + + ++
Sbjct: 318 DNLDLYMGTNPANDNFTFADANSLKPPSRVTNQRDADLVHFWEKYRKAPEGSARKTEAQK 377
Query: 332 ELENTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVL 391
++ + V + G FG+S +N R ++DD W
Sbjct: 378 QVLEAMSHRLHIDNSVILV----GKILFGISRGPEVLNKVRSAGQPLVDD----WNCLKN 429
Query: 392 LLDSTNR 398
+ + R
Sbjct: 430 QVRAFER 436
>sp|P49045|VPE_SOYBN VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||T07132 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - soybean
dbj|BAA06030.1| (D28876) cysteine proteinase [Glycine max]
Length = 495
Score = 612 bits (1564), Expect = e-174
Identities = 71/416 (17%), Positives = 139/416 (33%), Gaps = 45/416 (10%)
Query: 10 LCYSFVSSTGDTTI-HTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPDE 66
+ + WAVLV S H + + LL + G+ +E
Sbjct: 42 VIKLPTEPVDADSDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLI------KGGLKEE 95
Query: 67 RIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHE 126
I++ + DD+A N N + N+ +LY V DY G VT EN V+ G
Sbjct: 96 NIVVFMYDDIATNELNPRHGVIINHPE-GEDLYA-GVPKDYTGDNVTTENLFAVILGDKS 153
Query: 127 NAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKEL 185
S +++ S I +Y + HGG L + L + D D +K+ +KE+
Sbjct: 154 KLKGGSGKVINSKPEDRIFIYYSDHGGPGILGMPNMPYLYAMDFIDVLKKKHASGSYKEM 213
Query: 186 MIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----YYT 239
+I V+ C++ ++F + + +S ENS+ + + T Y+
Sbjct: 214 VIYVEACESGSVFEGIMPKDLNIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGDLYS 273
Query: 240 LAFFERLNIYDNASLNSLFRSYD----PRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVME 295
+A+ E N S+ + Y + + + Q + +
Sbjct: 274 VAWMEDSE-AHNLKRESVKQQYKSVKQRTSNFNNYAMGSHVMQYGDTNITAEKLYLYQGF 332
Query: 296 TIHT------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIPND 341
T + + +++R ++ D+ +++ T
Sbjct: 333 DPATVNFPPQNGRLETKMEVVNQRDAELFLLWQMYQRSNHQSENKTDILKQIAETVKHRK 392
Query: 342 ELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
+ V + + +G + R S++DD W ++
Sbjct: 393 HIDGSVELIGV----LLYGPGKGSSVLQSVRAPGSSLVDD----WTCLKSMVRVFE 440
>sp|P49044|VPE_VICSA VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) (PROTEINASE B)
pir||S49175 legumain (EC 3.4.22.34) precursor [similarity] - spring vetch
pir||S68984 cysteine proteinase precursor - spring vetch
emb|CAA84383.1| (Z34899) cysteine proteinase [Vicia sativa]
Length = 493
Score = 612 bits (1562), Expect = e-174
Identities = 77/423 (18%), Positives = 158/423 (37%), Gaps = 46/423 (10%)
Query: 4 LTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRL 61
L L F D WA+L+ S + H + + LL ++
Sbjct: 33 LRLPSETSRFFREPKNDDDFEGTRWAILLAGSNGYWNYRHQSDVCHAYQLL------RKG 86
Query: 62 GIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVL 121
G +E II+ + DD+A N N P + N + ++Y V DY G EV +NF L
Sbjct: 87 GSKEENIIVFMYDDIASNEENPRPGVIINKPD-GDDVYA-GVPKDYTGAEVHADNFYAAL 144
Query: 122 TGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
G S +++ S HI +Y T HGG L L + DL + +K+
Sbjct: 145 LGNKSALTGGSGKVVDSGPNDHIFVYYTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASG 204
Query: 181 RFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT-- 236
+K L+ ++ C++ ++F L + A +S E+S+ ++ D + T
Sbjct: 205 TYKSLVFYLEACESGSIFEGLLPDDLNIYATTASNAEESSWGYYCPGDKPPPPPEYSTCL 264
Query: 237 --YYTLAFFERLNIYDNASLNSLFRSYDPRLLMS-TAYYRTDLYQPHLVEVPVTNFFGSV 293
Y++A+ E + N SL + Y + + Y + + + + + + + +
Sbjct: 265 GDLYSIAWMEDSEV-HNLQTESLQQQYKLVKNRTISEPYGSHVMEYGDIGLSKNDLYQYL 323
Query: 294 METIHTDS-----------AYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELE 334
D+ + S+ +++R + + P +++ ++++
Sbjct: 324 GTNPANDNNSFVDETENSLKLRTPSAAVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVL 383
Query: 335 NTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLD 394
+ V + G FG+ +++ R ++D+ W ++
Sbjct: 384 EAMSHRKHIDNSVKLI----GQLLFGIEKGTELLDVVRPAGSPLVDN----WDCLKTMVK 435
Query: 395 STN 397
+
Sbjct: 436 TFE 438
>emb|CAB16318.1| (Z99174) cysteine proteinase precursor [Vicia narbonensis]
Length = 488
Score = 604 bits (1543), Expect = e-172
Identities = 75/417 (17%), Positives = 141/417 (32%), Gaps = 46/417 (11%)
Query: 10 LCYSFVSSTGDT--TIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPD 65
+ WAVLV S H + + LL + G+ +
Sbjct: 34 VIRLPGEPVDADVEDEMGTRWAVLVAGSNGYGNYRHQADVCHAYQLLI------KGGVKE 87
Query: 66 ERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRH 125
E I++ + DD+A N N P + N+ N+Y D V DY G VT ENF V+ G
Sbjct: 88 ENIVVFMYDDIAYNEMNPRPGVIINHP-QGPNVY-DGVPKDYNGDFVTAENFYAVILGDK 145
Query: 126 ENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKE 184
S +++ S I +Y + HGG L + + + D D +K+ +K+
Sbjct: 146 SKVRGGSGKVINSKAEDRIFIYCSDHGGPGVLGMPNMPYVYAMDFIDVLKKKHASGGYKK 205
Query: 185 LMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----YY 238
++I V+ C++ ++F + V +S ENS+ + + T Y
Sbjct: 206 MVIYVEACESGSIFEGIMPKDINVYVTTASNAQENSWGTYCPGVDPAPPPEYITCLGDLY 265
Query: 239 TLAFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVM 294
++A+ E N ++ + Y + + + Q + +
Sbjct: 266 SVAWMEDSET-HNLKRETVKQQYMSVRERTSNYKNYPLGSHVMQYGDTNITDEKLYLYHG 324
Query: 295 ETIH------TDSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIPN 340
+ +A +++R + + D E++ T
Sbjct: 325 FDPADGEPSSNNDILEAKMEVVNQRDAEILFMWHMYQRLDHQTEKKKDTLEKISETVKHR 384
Query: 341 DELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
+ L V + + FG + + R L ++DD W +
Sbjct: 385 NHLDGSVELIGV----LLFGPTKGSSVLQSVRASGLPLVDD----WECLKSRVRVFE 433
>emb|CAC43295.1| (AJ309173) putative vacuolar processing enzyme [Beta vulgaris]
Length = 486
Score = 604 bits (1542), Expect = e-172
Identities = 74/422 (17%), Positives = 157/422 (36%), Gaps = 47/422 (11%)
Query: 4 LTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRL 61
+ F D WAVL+ S + H + + +L K+
Sbjct: 31 IRWPSDHPSIF---ESDDDSVGTRWAVLIAGSSGYWNYRHQADVCHAYQVL------KKG 81
Query: 62 GIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVL 121
G+ DE II+ + DD+A + N P + N+ + ++Y V DY G +VTV NF +
Sbjct: 82 GLKDENIIVFMYDDIAYDEENPRPGVLINSP-YGHDVYA-GVPKDYTGEDVTVNNFFAAI 139
Query: 122 TGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
G + S +++ S HI ++ + HGG L L + +L + +K+
Sbjct: 140 LGNKDAITGGSGKVVNSGPNDHIFIFYSDHGGAGVLGMPTYPYLYADELIETLKEKHASG 199
Query: 181 RFKELMIMVDTCQAATLFNQLQS--PGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT-- 236
+K L++ ++ C++ ++F + + A +S E+S+ + + T
Sbjct: 200 TYKSLVVYIEACESGSIFEGILPEGLNIYATTASNAVESSWGTYCPGQDPNVPPEYDTCL 259
Query: 237 --YYTLAFFERLNIYDNASLNSLFRSYDPRLLMST--AYYRTDLYQPHLVEVPVTNFFGS 292
Y++++ E N SL + Y+ + +Y + + Q E+ +
Sbjct: 260 GDLYSVSWIEDSE-RHNLHTESLKQQYEVVKTKTAEKPFYGSHVMQYGDKELTQDMLYLY 318
Query: 293 VMETIHT-------DSAY-KAFSSKISERKIN--------SEMPFNQLSEHDLKEELENT 336
+ + D++ S+ +++R + + + + +++
Sbjct: 319 MGTNPNNENYTYVDDNSLHPTSSNAVNQRDADLIHFWNKFRKASEGSQRKINAQKQFMEV 378
Query: 337 NIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDST 396
L + + G FG+ L + R ++DD W L+ +
Sbjct: 379 MSHRVHLDDSIKLI----GKLLFGIEKGLGVLQTVRPTGQPLVDD----WNCLKTLVRTF 430
Query: 397 NR 398
+
Sbjct: 431 EK 432
>gb|AAF89646.1|AF169019_1 (AF169019) seed maturation protein PM40 [Glycine max]
Length = 496
Score = 603 bits (1540), Expect = e-171
Identities = 66/418 (15%), Positives = 138/418 (32%), Gaps = 47/418 (11%)
Query: 10 LCYSFVSSTGDTTIH--TNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPD 65
+ + H WAVLV S H + + LL + G+ +
Sbjct: 41 VIKLPAEPVDADSDHEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLI------KGGLKE 94
Query: 66 ERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRH 125
E I++ + DD+A + N P + N+ ++Y V DY G VT +N V+ G
Sbjct: 95 ENIVVFMYDDIATDELNPRPGVIINHPE-GQDVYA-GVPKDYTGENVTAQNLFAVILGDK 152
Query: 126 ENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKE 184
S +++ S I +Y + HGG L + L + D + +K+ +K+
Sbjct: 153 NKVKGGSGKVINSKPEDRIFIYYSDHGGPGVLGMPNMPYLYAMDFIEVLKKKHASGGYKK 212
Query: 185 LMIMVDTCQAAT-LFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----Y 237
++I V+ C++ + + + +S ENS+ + + T
Sbjct: 213 MVIYVEACESGNHVLKGIMPKDLQIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGDL 272
Query: 238 YTLAFFERLNIYDNASLNSLFRSYD----PRLLMSTAYYRTDLYQPHLVEVPVTNFFGSV 293
Y++A+ E N S+ + Y + + + Q + +
Sbjct: 273 YSVAWMEDSET-HNLKRESVKQQYKSVKQRTSNFNNYAMGSHVMQYGDTNITAEKLYLYQ 331
Query: 294 METIH------TDSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIP 339
+ + +++R + D+ +++ T
Sbjct: 332 GFDPAAVNFPPQNGRLETKMEVVNQRDAELFFMWQMYQRSNHQPEKKTDILKQIAETVKH 391
Query: 340 NDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
+ V + + +G + R L+++DD W ++
Sbjct: 392 RKHIDGSVELIGV----LLYGPGKGSSVLQSMRAPGLALVDD----WTCLKSMVRVFE 441
>sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE)
pir||JQ2387 vacuolar processing enzyme (EC 3.4.22.-) precursor - castor bean
dbj|BAA04225.1| (D17401) precursor of vacuolar processing enzyme [Ricinus communis]
Length = 497
Score = 600 bits (1532), Expect = e-170
Identities = 70/409 (17%), Positives = 141/409 (34%), Gaps = 44/409 (10%)
Query: 16 SSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLA 73
D WAVLV S H + + LL ++ G+ +E II+ +
Sbjct: 51 VQVDDDDQLGTRWAVLVAGSMGFGNYRHQADVCHAYQLL------RKGGLKEENIIVFMY 104
Query: 74 DDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSK 133
DD+A N N P + N+ ++Y V DY G VT +N VL G S
Sbjct: 105 DDIAKNELNPRPGVIINHP-QGEDVYA-GVPKDYTGEHVTAKNLYAVLLGDKSAVQGGSG 162
Query: 134 RLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTC 192
+++ S I LY + HGG L + L + D + +K+ +K+++I V+ C
Sbjct: 163 KVVDSKPNDRIFLYYSDHGGPGVLGMPNLPYLYAMDFIEVLKKKHAAGGYKKMVIYVEAC 222
Query: 193 QAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----YYTLAFFERL 246
++ ++F + + +S E+S+ + + T Y++A+ E
Sbjct: 223 ESGSIFEGIMPKDVDIYVTTASNAQESSWGTYCPGMEPSPPPEFTTCLGDLYSVAWMEDS 282
Query: 247 NIYDNASLNSLFRSYD----PRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIH---- 298
N ++ + Y +T + + Q + +
Sbjct: 283 ES-HNLKKETVKQQYSSVKARTSNYNTYAAGSHVMQYGNQSIKADKLYLFQGFDPASVNF 341
Query: 299 --TDSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIPNDELIAEVT 348
++ A +++R + ++ +++++ L + +
Sbjct: 342 PPNNAHLNAPMEVVNQRDAELHFMWQLYKRSENGSEKKKEILQQIKDAIKHRSHLDSSMQ 401
Query: 349 VYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
+ G FG + R ++DD W ++
Sbjct: 402 LI----GDLLFGPKKASAILKSVREPGSPLVDD----WGCLKSMVRVFE 442
>sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptidase)
pir||JX0344 legumain (EC 3.4.22.34) precursor - jack bean
dbj|BAA06596.1| (D31787) asparaginyl endopeptidase (Legumain) [Canavalia
ensiformis]
Length = 475
Score = 600 bits (1531), Expect = e-170
Identities = 71/415 (17%), Positives = 147/415 (35%), Gaps = 46/415 (11%)
Query: 10 LCYSFVSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPDER 67
+ D WAVLV S H + + LL + G+ +E
Sbjct: 25 VIQLPTEPVDDE--VGTRWAVLVAGSNGYGNYRHQADVCHAYQLLI------KGGVKEEN 76
Query: 68 IILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHEN 127
I++ + DD+A NA N P + N+ ++Y V DY G +VT EN V+ G
Sbjct: 77 IVVFMYDDIAYNAMNPRPGVIINHP-QGPDVYA-GVPKDYTGEDVTPENLYAVILGDKSK 134
Query: 128 AVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELM 186
S +++ S+ I ++ + HGG L +A + + D D +K+ +KE++
Sbjct: 135 VKGGSGKVINSNPEDRIFIFYSDHGGPGVLGMPNAPFVYAMDFIDVLKKKHASGGYKEMV 194
Query: 187 IMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----YYTL 240
I ++ C++ ++F + + +S ENS+ + + T Y++
Sbjct: 195 IYIEACESGSIFEGIMPKDLNIYVTTASNAQENSFGTYCPGMNPPPPEEYVTCLGDLYSV 254
Query: 241 AFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMET 296
++ E N ++ + Y ++ + + + Q + +
Sbjct: 255 SWMEDSET-HNLKRETVQQQYQSVRKRTSNSNSYRFGSHVMQYGDTNITAEKLYLYHGFD 313
Query: 297 IHT------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIPNDE 342
T + +A +++R + + E++ T +
Sbjct: 314 PATVNFPPHNGNLEAKMEVVNQRDAELLFMWQMYQRSNHQPEKKTHILEQITETVKHRNH 373
Query: 343 LIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
L V + + +G ++ R L ++DD W ++
Sbjct: 374 LDGSVELIGV----LLYGPGKSSSVLHSVRAPGLPLVDD----WTCLKSMVRVFE 420
>ref|NP_180165.1| (NM_128154) putative vacuolar processing enzyme [Arabidopsis
thaliana]
sp|P49047|VPEA_ARATH VACUOLAR PROCESSING ENZYME, ALPHA-ISOZYME PRECURSOR (ALPHA-VPE)
pir||T02629 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
gb|AAC31241.1| (AC004747) vacuolar processing enzyme, alpha-isozyme precursor
(alpha-VPE) [Arabidopsis thaliana]
dbj|BAA09614.2| (D61393) alpha-VPE [Arabidopsis thaliana]
gb|AAL24163.1| (AY058055) At2g25940/F17H15.3 [Arabidopsis thaliana]
Length = 478
Score = 596 bits (1523), Expect = e-169
Identities = 79/423 (18%), Positives = 157/423 (36%), Gaps = 47/423 (11%)
Query: 3 ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKR 60
++ L L F + D WAVLV S + H + + LL K+
Sbjct: 22 VIKLPSLASKFFRPTENDDDS--TKWAVLVAGSSGYWNYRHQADVCHAYQLL------KK 73
Query: 61 LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 120
G+ +E I++ + DD+A N N P + N+ N ++Y + V DY G EV V+N L V
Sbjct: 74 GGVKEENIVVFMYDDIAKNEENPRPGVIINSPN-GEDVY-NGVPKDYTGDEVNVDNLLAV 131
Query: 121 LTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEK 179
+ G S +++ S HI +Y + HGG L + L ++DL D +K+
Sbjct: 132 ILGNKTALKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYAS 191
Query: 180 RRFKELMIMVDTCQAATLFNQLQS--PGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT- 236
+K L+ ++ C++ ++F L + A +S E+S+ + + + T
Sbjct: 192 GTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPSEYETC 251
Query: 237 ---YYTLAFFERLNIYDNASLNSLFRSYDPRLLM---STAYYRTDLYQPHLVEVPVTNFF 290
Y++A+ E N +L Y+ S Y + + + + +
Sbjct: 252 LGDLYSVAWIEDSE-KHNLQTETLHEQYELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLV 310
Query: 291 GSVMETIH-------TDSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELEN 335
+ +++ + S ++R + + P + + ++++
Sbjct: 311 LFMGTNPADENFTFVNENSIRPPSRVTNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLE 370
Query: 336 TNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDS 395
+ + + +N R ++DD W L+ +
Sbjct: 371 AMSHRLHVDNSILLIG-----ILLFGLEGHAVLNKVRPSGEPLVDD----WDCLKSLVRA 421
Query: 396 TNR 398
R
Sbjct: 422 FER 424
>pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17079.1| (Z99957) legumain-like proteinase precursor [Phaseolus vulgaris]
Length = 493
Score = 595 bits (1519), Expect = e-169
Identities = 74/416 (17%), Positives = 139/416 (32%), Gaps = 45/416 (10%)
Query: 10 LCYSFVSSTGDTTI-HTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPDE 66
+ + WAVLV S H + + LL + G+ +E
Sbjct: 40 VIKLPTQEVDAESDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLI------KGGVKEE 93
Query: 67 RIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHE 126
I++ + DD+A + N P + NN ++Y V DY G VT NF VL G
Sbjct: 94 NIVVFMYDDIATHELNPRPGVIINNP-QGPDVYA-GVPKDYTGESVTSHNFFAVLLGDKS 151
Query: 127 NAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKEL 185
S +++ S I +Y + HGG L + L + D D +K+ +KE+
Sbjct: 152 KVKGGSGKVINSKPEDRIFVYYSDHGGPGVLGMPNMPYLYAMDFIDVLKKKHASGGYKEM 211
Query: 186 MIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----YYT 239
+I V+ C++ ++F + + +S ENS+ + + T Y+
Sbjct: 212 VIYVEACESGSIFEGIMPKDLNIYVTTASNAQENSWGTYCPGMYPPPPPEYITCLGDLYS 271
Query: 240 LAFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVME 295
+A+ E N S+ + Y + + Q + +
Sbjct: 272 VAWMEDSES-HNLKKESVEQQYQSVKQRTSNFEAYAMGSHVMQYGDANMTAEKLYLYHGF 330
Query: 296 TIHT------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIPND 341
T + K+ +++R + D+ +++E
Sbjct: 331 DPATVNFPPHNGRLKSKMEVVNQRDAELLFMWQVYQRSNHLPEKKTDILKQIEEIVKHRK 390
Query: 342 ELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
L V + + +G + R L ++DD W ++
Sbjct: 391 HLDGSVELIGV----LLYGPEKASSVLRSVRTTGLPLVDD----WTCLKSMVRVYE 438
>gb|AAF89679.1| (AF169973) asparaginyl endopeptidase [Sesamum indicum]
Length = 489
Score = 595 bits (1519), Expect = e-169
Identities = 75/422 (17%), Positives = 151/422 (35%), Gaps = 52/422 (12%)
Query: 3 ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKR 60
I+ + +T + WAVLV S H + + +L K+
Sbjct: 38 IIRWPLDRR--------ETEDNATRWAVLVAGSNGFGNYRHQADVCHAYQIL------KK 83
Query: 61 LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 120
G+ DE II+ + DD+A N N + N+ ++Y V DY G +VT EN V
Sbjct: 84 GGLRDENIIVFMYDDIAMNELNPRKGVIINHPTGG-DVYA-GVPKDYTGEQVTAENLYAV 141
Query: 121 LTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEK 179
+ G S +++ S I +Y + HGG L + L ++D + +K+
Sbjct: 142 ILGDKSAIKGGSGKVVDSKPNDRIFIYYSDHGGPGVLGMPNMPYLYANDFIEVLKKKHAS 201
Query: 180 RRFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT- 236
+KE++I V+ C++ ++F L + +S E+S+ + + T
Sbjct: 202 GTYKEMVIYVEACESGSVFEGLMPDDLDIYVTTASNAEESSWGTYCPGMDPPPPPEYITC 261
Query: 237 ---YYTLAFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNF 289
Y++A+ E N ++ + Y + +T + + + +
Sbjct: 262 LGDLYSVAWMEDSES-HNLKRETVEQQYQQVKERTSNFNTYNAGSHVMEYGNKSIKSEKL 320
Query: 290 FGSVMETIHT------DSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELEN 335
+ T ++ K +++R + + + +L + + +
Sbjct: 321 YLYQGFDPATENMPPSENHLKPHMDVVNQRDADLLFLWERYKRLDGGAKKKSELFKLITD 380
Query: 336 TNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDS 395
T + + + + F FG + R R L + DD W ++
Sbjct: 381 TMLHRKHMDDSIDIIGAF----LFGPENGPSILKSVRDRGLPLADD----WDCLKSMVRL 432
Query: 396 TN 397
Sbjct: 433 FE 434
>pir||S60049 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
Length = 478
Score = 590 bits (1507), Expect = e-167
Identities = 78/423 (18%), Positives = 157/423 (36%), Gaps = 47/423 (11%)
Query: 3 ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKR 60
++ L L F + D WAVLV S + H + + L K+
Sbjct: 22 VIKLPSLASKFFRPTENDDDS--TKWAVLVAGSSGYWNYRHQADVCHAYQRL------KK 73
Query: 61 LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 120
G+ +E I++++ DD+A N N P + N+ N ++Y + V DY G EV V+N L V
Sbjct: 74 GGVKEENIVVLMYDDIAENEENPRPGVIINSPN-GEDVY-NGVPKDYTGDEVNVDNLLAV 131
Query: 121 LTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEK 179
+ G S +++ S HI +Y + HGG L + L ++DL D +K+
Sbjct: 132 ILGNKTALKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYAS 191
Query: 180 RRFKELMIMVDTCQAATLFNQLQS--PGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT- 236
+K L+ ++ C++ ++F L + A +S E+S+ + + + T
Sbjct: 192 GTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPSEYETC 251
Query: 237 ---YYTLAFFERLNIYDNASLNSLFRSYDPRLLM---STAYYRTDLYQPHLVEVPVTNFF 290
Y++A+ E N +L Y+ S Y + + + + +
Sbjct: 252 LGDLYSVAWIEDSE-KHNLQTETLHEQYELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLV 310
Query: 291 GSVMETIH-------TDSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELEN 335
+ +++ + S ++R + + P + + ++++
Sbjct: 311 LFMGTNPADENFTFVNENSIRPPSRVTNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLE 370
Query: 336 TNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDS 395
+ + + +N R ++DD W L+ +
Sbjct: 371 AMSHRLHVDNSILLIG-----ILLFGLEGHAVLNKVRPSGEPLVDD----WDCLKSLVRA 421
Query: 396 TNR 398
R
Sbjct: 422 FER 424
>pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring vetch
emb|CAA07639.1| (AJ007743) cysteine proteinase precursor [Vicia sativa]
Length = 503
Score = 588 bits (1501), Expect = e-167
Identities = 71/418 (16%), Positives = 146/418 (33%), Gaps = 47/418 (11%)
Query: 10 LCYSFVSSTGDT--TIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPD 65
+ WAVLV S H + + LL + G+ +
Sbjct: 48 VIRLPGEPVDADVEDEIGTRWAVLVAGSNGYGNYRHQADVCHAYQLLI------KGGVKE 101
Query: 66 ERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRH 125
E I++ + DD+A + N P + N+ N+Y D V DY G VT +N V+ G
Sbjct: 102 ENIVVFMYDDIAYSEFNPRPGVIINHP-QGPNVY-DGVPKDYTGDFVTADNLYAVILGDK 159
Query: 126 ENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKE 184
S +++ S I +Y + HGG L + + + D D +K+ R +++
Sbjct: 160 SKVRGGSGKVINSKAEDRIFIYYSDHGGPGVLGMPNMPYVYAMDFIDVLKKKHASRGYQQ 219
Query: 185 LMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHL-DSDIGVSVVDRFT----Y 237
++I V+ C++ ++F + V +S E+S+ +L S + T
Sbjct: 220 MVIYVEACESGSVFQGIMPKDIDVYVTTASNAEESSWGTYLVPGVYPASPPEYITCLGDL 279
Query: 238 YTLAFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSV 293
Y++A+ E N +L + + + L + + + + + +
Sbjct: 280 YSVAWMEDSET-HNLKRETLKQQFASVKERTLNNNNYGLGSHVTEYGDTNITDEKLYLYH 338
Query: 294 METIH------TDSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIP 339
+ ++ +++R + + D+ +++ T
Sbjct: 339 GFDPASVNLPPNNGRLESKMEVVNQRDAEILFMWQMYQRLDHQSEKKRDILKKISETVKH 398
Query: 340 NDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
+ L V + + FG + + R L ++DD W +
Sbjct: 399 RNHLDGSVELIGV----LLFGPTRGSSVLQSVRASGLPLVDD----WECLKSRVRVFE 448
>pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme beta precursor -
Arabidopsis thaliana
dbj|BAA09615.1| (D61394) beta-VPE [Arabidopsis thaliana]
Length = 484
Score = 582 bits (1485), Expect = e-165
Identities = 78/403 (19%), Positives = 148/403 (36%), Gaps = 44/403 (10%)
Query: 20 DTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLADDMA 77
D WAVLV S H + + +L ++ G+ +E I++++ DD+A
Sbjct: 44 DEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQIL------RKGGLKEENIVVLMYDDIA 97
Query: 78 CNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRLL- 136
+ N P + N+ + ++Y V DY G VT NF VL G + S +++
Sbjct: 98 NHPLNPRPGTLINHPD-GDDVYA-GVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIA 155
Query: 137 SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAAT 196
S HI +Y HGG L + + + D + +K+ +KE++I V+ C++ +
Sbjct: 156 SKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGS 215
Query: 197 LFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----YYTLAFFERLNIYD 250
+F + + +S E+SY + + T Y++A+ E
Sbjct: 216 IFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSET-H 274
Query: 251 NASLNSLFRSYD--PRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD----SAYK 304
N ++ + Y +T + + + + + T +
Sbjct: 275 NLKKETIKQQYHTMRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGFEPATVNLPLNELP 334
Query: 305 AFSS--KISERKIN--------SEMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFP 354
A S +++R + + D +EL T L A V +
Sbjct: 335 AKSKIGVVNQRDADLLFLWHMHRTSEDGSRKKDDTLKELTETTRHRKHLDASVELIAT-- 392
Query: 355 GLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
FG + + +NL R L ++DD W ++
Sbjct: 393 --ILFGPT--MNVLNLVREPGLPLVDD----WECLKSMVRVFE 427
>ref|NP_176458.1| (NM_104948) beta-VPE [Arabidopsis thaliana]
gb|AAK56243.1|AF367254_1 (AF367254) At1g62710/F23N19_8 [Arabidopsis thaliana]
gb|AAL15381.1| (AY059156) At1g62710/F23N19_8 [Arabidopsis thaliana]
Length = 486
Score = 581 bits (1484), Expect = e-165
Identities = 77/405 (19%), Positives = 147/405 (36%), Gaps = 46/405 (11%)
Query: 20 DTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLADDMA 77
D WAVLV S H + + +L ++ G+ +E I++++ DD+A
Sbjct: 44 DEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQIL------RKGGLKEENIVVLMYDDIA 97
Query: 78 CNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRLL- 136
+ N P + N+ + ++Y V DY G VT NF VL G + S +++
Sbjct: 98 NHPLNPRPGTLINHPD-GDDVYA-GVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIA 155
Query: 137 SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAAT 196
S HI +Y HGG L + + + D + +K+ +KE++I V+ C++ +
Sbjct: 156 SKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGS 215
Query: 197 LFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----YYTLAFFERLNIYD 250
+F + + +S E+SY + + T Y++A+ E
Sbjct: 216 IFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSET-H 274
Query: 251 NASLNSLFRSYD----PRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD----SA 302
N ++ + Y +T + + + + + T +
Sbjct: 275 NLKKETIKQQYHTVKMRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGFDPATVNLPLNE 334
Query: 303 YKAFSS--KISERKIN--------SEMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTL 352
S +++R + + D +EL T L A V +
Sbjct: 335 LPVKSKIGVVNQRDADLLFLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLDASVELIAT 394
Query: 353 FPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
FG + + +NL R L ++DD W ++
Sbjct: 395 ----ILFGPT--MNVLNLVREPGLPLVDD----WECLKSMVRVFE 429
>emb|CAB42655.1| (AJ238882) putative preprolegumain [Vicia narbonensis]
Length = 380
Score = 572 bits (1461), Expect = e-162
Identities = 69/379 (18%), Positives = 146/379 (38%), Gaps = 42/379 (11%)
Query: 44 VLTFIFSLLGVSRTVKRLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNV 103
+ + LL ++ G+ +E II+ + DD+A + N P + N+ H N+Y + V
Sbjct: 1 DVCHAYQLL------RKGGLKEENIIVFMYDDIAYSEENPRPGVIINSP-HGENVY-EGV 52
Query: 104 EVDYRGYEVTVENFLRVLTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAE 162
DY G +VTV NF L G S +++ S I ++ + HGG L +
Sbjct: 53 PKDYTGEDVTVGNFFAALLGNKSALSGGSGKVVDSGPNDRIFVFYSDHGGPGVLGMPTSP 112
Query: 163 ELQSHDLADAVKQMKEKRRFKELMIMVDTCQAATLFNQLQS--PGVLAIGSSLKGENSYS 220
+ + DL + +K +K L+ ++ C++ ++F L + A ++ E+S+
Sbjct: 113 YMYASDLVEVLKIKHAAGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTAANAEESSWG 172
Query: 221 HHLDSDIGVSVVDRFT----YYTLAFFERLNIYDNASLNSLFRSYDPRLLMS---TAYYR 273
+ + + T Y++A+ E +I N +L + Y+ + + Y
Sbjct: 173 TYCPGENPSPPPEYETCLADLYSVAWMEDSDI-HNLQTETLHQQYELVKERTSNGNSNYG 231
Query: 274 TDLYQPHLVEVPVTNFFGSVMETIHTD-------SAYKAFSSKISERKIN--------SE 318
+ + Q +E+ + F + + ++ S I++R + +
Sbjct: 232 SHVMQYGDIELSKDSLFLYLGSNPSNENFTFVGRNSLVPPSKAINQRDADLIHFWDKFRK 291
Query: 319 MPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSM 378
P + ++E+ + + + G FG+ + R +
Sbjct: 292 APQGSPRKAAAQKEVLEAMSHRMHIDDSIKLV----GKLLFGMKKGPEVLTSVRPAGQPL 347
Query: 379 IDDVFAFWLVFVLLLDSTN 397
+DD W L+ +
Sbjct: 348 VDD----WDCLKTLVRTFE 362
>emb|CAB42651.1| (AJ238881) putative preprolegumain [Nicotiana tabacum]
Length = 419
Score = 558 bits (1423), Expect = e-158
Identities = 72/381 (18%), Positives = 142/381 (36%), Gaps = 41/381 (10%)
Query: 41 HSLVLTFIFSLLGVSRTVKRLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYG 100
H + + +L KR G+ DE I++ + DD+A + N P + N+ N ++Y
Sbjct: 1 HQADVCHAYQIL------KRGGLKDENIVVFMYDDIAKSELNPRPGVIINHPN-GSDVYA 53
Query: 101 DNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQ 159
V DY G VT N VL G S +++ S I LY + HGG L
Sbjct: 54 -GVPKDYTGEHVTAANLYAVLLGDKSAVKGGSGKIVDSKPNDRIFLYYSDHGGPGVLGMP 112
Query: 160 DAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGEN 217
+ L + D + +K+ +KE+++ ++ C++ ++F + + +S E+
Sbjct: 113 NMPFLYAKDFIEVLKKKHAAGTYKEMVLYIEACESGSVFEGMMPEDLNIYVTTASNAEES 172
Query: 218 SYSHHLDSDIGVSVVDRFT----YYTLAFFERLNIYDNASLNSLFRSY----DPRLLMST 269
S+ + + T Y++A+ E N ++ + Y + +
Sbjct: 173 SWGTYCPGMDPPPPPEYITCLGDLYSVAWMEDSES-HNLKKETIKQQYEKVKERTSNFNN 231
Query: 270 AYYRTDLYQPHLVEVPVTNFFGSVMETIHTDSAYK-----AFSSKISERKIN-------- 316
+ + + E+ + T + A +++R +
Sbjct: 232 YNAGSHVMEYGSKEIKPEKVYLYQGFDPATANLSANKIAFAHVEVVNQRDADLLFLWERY 291
Query: 317 SEMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVL 376
E+ N L + L++E+ +T + L V +F FG + +N R L
Sbjct: 292 KELADNSLEKAKLRKEITDTMLHRKHLDGSVDAIGVF----LFGPTKGSSVLNSVREPGL 347
Query: 377 SMIDDVFAFWLVFVLLLDSTN 397
++DD W +
Sbjct: 348 PLVDD----WDCLKSTVRLFE 364
>gb|AAL40390.1| (AY062178) C13 cysteine proteinase precursor [Oryza sativa subsp.
indica] [Oryza sativa (indica cultivar-group)]
Length = 465
Score = 542 bits (1384), Expect = e-153
Identities = 59/415 (14%), Positives = 134/415 (32%), Gaps = 59/415 (14%)
Query: 17 STGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLAD 74
+ WAVLV S H + + +L ++ G+ +E I++ + D
Sbjct: 21 EEEEKDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQIL------RKGGLKEENIVVFMYD 74
Query: 75 DMACNARNEYPA--QVFNNENHKLNLYGDNVEVDYRGYEVTVE------NFLRVLTGRHE 126
D+A N N P + + + + +T+E NF VL G
Sbjct: 75 DIANNILNPRPGLLSIIHRVKMFMQEF----------RRITLETKSLQKNFYAVLLGNKT 124
Query: 127 NAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKEL 185
S++++ S HI ++ + HGG L + L + D +++ + ++
Sbjct: 125 AVTGGSRKVIDSKPNDHIFIFYSDHGGPGVLGMPNLPYLYAADFMKVLQEKHASNTYAKM 184
Query: 186 MIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT----YYT 239
+I V+ C++ ++F L + +S E+S+ + + T Y+
Sbjct: 185 VIYVEACESGSIFEGLMPEDLNIYVTTASNAEESSWGTYCPGMEPSPPSEYITCLGDLYS 244
Query: 240 LAFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVME 295
+++ E N S+ + Y M++ + + + +
Sbjct: 245 VSWMEDSET-HNLKEESIKKQYEVVKKRTSDMNSYGAGSHVMEYGDRTFKDDKLYLYQGF 303
Query: 296 TIHT---DSAY--KAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIPNDE 342
+ + + ++R + + + E+ +T +
Sbjct: 304 DPANAEVKNKLSWEGPKAAANQRDADLLFLWRRYELLHDKSEEKLKALREISDTVMHRKL 363
Query: 343 LIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
L + V + G G + ++DD W ++
Sbjct: 364 LDSSVDLV----GKLLLGFGNGPSVLQAVSPSGQPLVDD----WDCLKRMVRIFE 410
>emb|CAB51545.1| (AJ243876) vacuolar processing enzyme [Lycopersicon esculentum]
Length = 460
Score = 528 bits (1347), Expect = e-149
Identities = 75/414 (18%), Positives = 152/414 (36%), Gaps = 55/414 (13%)
Query: 17 STGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLAD 74
WAVLV S+ + H L + LL K+ G+ DE II+ + D
Sbjct: 29 DEDYEDSIGTKWAVLVAGSKEWYNYRHQANLCHAYQLL------KKGGLKDEHIIVFMYD 82
Query: 75 DMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKR 134
D+A N N P + NN H ++Y V DY G + +NF V+ G S +
Sbjct: 83 DIANNPENPRPGVIINNP-HGHDVY-KGVPKDYTGKDCNAQNFYSVILGNKSALTGGSGK 140
Query: 135 LL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQ 193
++ S +I +Y T HG + + + + DL + +K+ R +K+++ ++ C
Sbjct: 141 VVNSGPNDYIFIYYTDHGAPGLVGMPEDPPVYAIDLNEVLKKKHASRTYKKMVFYLEACD 200
Query: 194 AATLFNQLQS--PGVLAIGSSLKGENSYSHHL-----DSDIGVSVVDRFT------YYTL 240
+ ++F L + A SS E+ ++ + S G F +++
Sbjct: 201 SGSMFADLLDEGLNIYATTSSKPDEDGWATYCYFTGDTSCYGECPPKDFKDNCLGDLFSV 260
Query: 241 AFFERLNIYDNASLNSLFRSY----DPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMET 296
++ E ++ + + +L + Y L T + + + Q + + +
Sbjct: 261 SWLENSDL-HDLQVETLEKQYLRIHKRVLNNGT--HGSHMVQYGDLHINKDALSIYMGSN 317
Query: 297 IH-----TDSAYKAFSSKISERKIN--------SEMPFNQLSEHDLKEELENTNIPNDEL 343
++ + S +++R + P +++ +L + +
Sbjct: 318 SPKHTWSANNNNASNSRHVNQRDVQLLYLISKFQNAPEGSRRKNEAYRKLSEVISEREHV 377
Query: 344 IAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
V G FG+ + +N+ R ++DD W +
Sbjct: 378 DKSVKHI----GQILFGVENGQKVLNIVR---QPLVDD----WDCLKSFVKIFE 420
>gb|AAD04882.1| (AF082346) C13 endopeptidase NP1 precursor [Hordeum vulgare]
Length = 411
Score = 523 bits (1334), Expect = e-147
Identities = 64/375 (17%), Positives = 137/375 (36%), Gaps = 40/375 (10%)
Query: 44 VLTFIFSLLGVSRTVKRLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNV 103
+ + +L K+ G+ DE I++ + DD+A + N P V N+ ++Y V
Sbjct: 1 DVCHAYQIL------KKGGLKDENIVVFMYDDIANSPDNPRPGIVINHPK-GKDVYH-GV 52
Query: 104 EVDYRGYEVTVENFLRVLTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAE 162
DY G +VT +NF VL G S++++ S HI +Y T HG L +
Sbjct: 53 PKDYTGDQVTAKNFYAVLLGNKTAVTGGSRKVVNSKPNDHIFIYYTDHGAAGLLCMPNPP 112
Query: 163 ELQSHDLADAVKQMKEKRRFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYS 220
++ + D ++Q + + +++I V+ C++ ++F L + ++ ENS++
Sbjct: 113 DVYADDFIKVLRQKHASKSYSKMIIYVEACESGSIFEGLMPQDHNIYVTTAANAQENSWA 172
Query: 221 HHLDSDIGVSVVDRFT----YYTLAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDL 276
+ + + T Y++++ E D S+ + Y+ + + +
Sbjct: 173 AYCPTMEIPPPPEYDTCLGDAYSVSWMEDSETQD-LKNESIKQQYEVVKERTAPL--SHV 229
Query: 277 YQPHLVEVPVTNFFGSVMETIHTDSA------YKAFSSKISERKIN--------SEMPFN 322
+ F S + I++R + ++
Sbjct: 230 MEYGDKTFKEDMLFLFQGFDPAKSSIRNRPLPMPSLKGAINQRDADILFMWRKYEKLNAG 289
Query: 323 QLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDV 382
+ E++ T + L + + G FG + +R ++DD
Sbjct: 290 SEEKQRALREVKETVLHRKHLDSSIDFI----GKLVFGFDKGPSMLQAARGSGQPLVDD- 344
Query: 383 FAFWLVFVLLLDSTN 397
W ++
Sbjct: 345 ---WDCLRTMVRVFE 356
>ref|NP_563825.1| (NM_100749) putative GPI-anchor transamidase (U32517) [Arabidopsis
thaliana]
Length = 388
Score = 511 bits (1304), Expect = e-144
Identities = 329/346 (95%), Positives = 332/346 (95%), Gaps = 4/346 (1%)
Query: 1 MKILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKR 60
MKILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRF + + L + RTVKR
Sbjct: 1 MKILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFW----FNYRHMANTLSLYRTVKR 56
Query: 61 LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 120
LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV
Sbjct: 57 LGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRV 116
Query: 121 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR
Sbjct: 117 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 176
Query: 181 RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL 240
RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL
Sbjct: 177 RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL 236
Query: 241 AFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD 300
AFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD
Sbjct: 237 AFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD 296
Query: 301 SAYKAFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAE 346
SAYKAFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAE
Sbjct: 297 SAYKAFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAE 342
>emb|CAB42650.1| (AJ238880) putative preprolegumain [Nicotiana tabacum]
Length = 400
Score = 507 bits (1293), Expect = e-142
Identities = 63/380 (16%), Positives = 140/380 (36%), Gaps = 43/380 (11%)
Query: 41 HSLVLTFIFSLLGVSRTVKRLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYG 100
H + + LL K+ G+ DE II+ + DD+A N N P + N+ H ++Y
Sbjct: 1 HQADVCHAYQLL------KKGGLKDENIIVFMYDDIAYNKNNPRPGIIINSP-HGHDVY- 52
Query: 101 DNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQ 159
V DY G + +NF V+ G S +++ + +I +Y HG +
Sbjct: 53 KGVPKDYTGKDCNADNFFAVILGNKSALTGGSGKVVENGPNDYIFIYYADHGAPGLIGMP 112
Query: 160 DAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAATLFNQLQS--PGVLAIGSSLKGEN 217
+ + + DL + + + +L+ ++ C++ ++F+ L + +S E+
Sbjct: 113 SGDVVYADDLNRVLIKKHTFGTYSKLVFYMEACESGSMFDGLLPKGLNIYVTAASKPDES 172
Query: 218 SYSHHLD--SDIGVSVVDRFTYYTLAFFERLNIYDNASLNSLFRSYD---PRLLMSTAYY 272
S++ + D + D Y++++ E ++ + + +L + Y R L +
Sbjct: 173 SWATYCIRLGDEDQCLGD---LYSVSWLEDSDL-HDRQVETLEKQYQLVRKRTLNNGTEE 228
Query: 273 RTDLYQPHLVEVPVTNFFGSVMETIH-------TDSAYKAFSSKISERKIN--------S 317
+ + Q + + F + + S +++R ++
Sbjct: 229 GSHVMQYGDLHISEDPLFRYMGSNSAKNSYNTSNNDESWLPSRTVNQRDVHLMHLWSKFR 288
Query: 318 EMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLS 377
P + + +L +++ V G FG+ + +N R
Sbjct: 289 SAPEGSARKAEAHRQLSEALSQREDVDNSVRHI----GEVLFGVEKSHKLLNTVRPAGQP 344
Query: 378 MIDDVFAFWLVFVLLLDSTN 397
++DD W +
Sbjct: 345 LVDD----WDCLKSFVKIFE 360
>ref|NP_188656.1| (NM_112912) vacuolar processing enzyme/asparaginyl endopeptidase,
putative [Arabidopsis thaliana]
dbj|BAB01880.1| (AP000383) vacuolar processing enzyme (proteinase) [Arabidopsis
thaliana]
Length = 466
Score = 494 bits (1261), Expect = e-139
Identities = 78/412 (18%), Positives = 171/412 (40%), Gaps = 52/412 (12%)
Query: 16 SSTGDTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLA 73
+ D + WAVLV S + H + + +L ++ G+ DE II+ +
Sbjct: 35 VESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQIL------RKGGLKDENIIVFMY 88
Query: 74 DDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSK 133
DD+A ++ N P + N + ++Y V DY V V+NF VL G +
Sbjct: 89 DDIAFSSENPRPGVIINKPD-GEDVY-KGVPKDYTKEAVNVQNFYNVLLGNESGVTGGNG 146
Query: 134 RLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTC 192
+++ S +I +Y HG + +E+ + D + +++M +++++ +++I V+ C
Sbjct: 147 KVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRKKYNKMVIYVEAC 206
Query: 193 QAATLFNQLQ--SPGVLAIGSSLKGENSYS-------HHLDSDIGVSVVDRFTYYTLAFF 243
++ ++F + + + A+ ++ E+S+ S+IG + D F ++++
Sbjct: 207 ESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIGTCLGDTF---SISWL 263
Query: 244 ERLNIYDNASLNSLFRSYD--PRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTDS 301
E ++ + S +L + Y R + S + + + ++ + D+
Sbjct: 264 EDSDL-HDMSKETLEQQYHVVKRRVGSDVPETSHVCRFGTEKMLKDYLSSYIGRNPENDN 322
Query: 302 --AYKAFSSKIS---------------ERKINSEMPFNQLSEHDLKEELENTNIPNDELI 344
++FSS IS +RKI + P L + +++L + ++
Sbjct: 323 FTFTESFSSPISNSGLVNPRDIPLLYLQRKI-QKAPMGSLESKEAQKKLLDEKNHRKQID 381
Query: 345 AEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDST 396
+T + +L + +R ++DD W F L++S
Sbjct: 382 QSITDILRLSVKQ----TNVLNLLTSTRTTGQPLVDD----WDCFKTLVNSF 425
>dbj|BAA90621.1| (AP001129) Similar to Phaseolus vulgaris Moldavian encoding
asparagine-specific endopeptidase precursor (Z99956)
[Oryza sativa]
Length = 452
Score = 487 bits (1241), Expect = e-136
Identities = 75/396 (18%), Positives = 155/396 (38%), Gaps = 43/396 (10%)
Query: 21 TTIHTNNWAVLVCTSRF--CSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLADDMAC 78
T WA+L+ S+ H + + ++ K+ G+ D+ I++M+ DD+A
Sbjct: 35 TENGGTKWALLIAGSKGYENYRHQADVCHAYQIM------KKGGLKDQNIVVMMYDDIAY 88
Query: 79 NARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVP-RSKRLL- 136
N N + + N N N+Y V DY G +V NFL VL G+ S +++
Sbjct: 89 NPENPHKGVIINKPN-GPNVYA-GVPKDYNGNDVNKNNFLAVLLGKKSALTGAGSGKVIS 146
Query: 137 SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAAT 196
S HI +Y + HG ++ L ++DL+ A+K +K L++ V+ C++ +
Sbjct: 147 SGPNDHIFVYYSDHGSPGYVCMPSGGNLHANDLSQALKNKNAAGAYKNLVVYVEACESGS 206
Query: 197 LFNQLQ---SPGVLAIGSSLKGENSYSHHLD-SDIGVSVVDRFTYYTLAFFERLNIYDNA 252
+F + GV A+ +S ENS++ + D + + D +++A+ E +
Sbjct: 207 MFEGQLLPSNIGVYAMTASNATENSWATYCDTPEYNTCLGD---LFSVAWMEDADARRPG 263
Query: 253 SLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFG-SVMETIHTDSAYKAFSSK-- 309
+L + YD + + + + + + + T +A +
Sbjct: 264 DPETLGQLYDIVAKRTNL---SHVSRYGDLSLSSQPVSLYYLPPGPGTSTASAVIDDEGR 320
Query: 310 ---ISERKIN-----SEMPFNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFGL 361
+++R + + E L + + V + G G
Sbjct: 321 VGGVNQRDAGLVYLWRKYYE--EKSVEAWERLLREMERRSRVDSSVDLI----GDILLGD 374
Query: 362 STLLRYMNLSRVRVLSMIDDVFAFWLVFVLLLDSTN 397
S+ + +++ R ++DD W ++ +
Sbjct: 375 SSKKKLLHIRRPAGQPLVDD----WDCLKSMVRTFE 406
>dbj|BAA09530.1| (D55696) cysteine protease [Homo sapiens]
Length = 433
Score = 443 bits (1128), Expect = e-123
Identities = 82/356 (23%), Positives = 148/356 (41%), Gaps = 48/356 (13%)
Query: 2 KILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFC--SLHSLVLTFIFSLLGVSRTVK 59
K+ + + D +WAV+V S H + + +
Sbjct: 4 KVAVFLSVALGIGAVPIDDPEDGGKHWAVIVAGSNGWYNYRHQADACHAYQI------IH 57
Query: 60 RLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLR 119
R GIPDE+I++M+ DD+A + N P V N N ++Y V DY G +VT +NFL
Sbjct: 58 RNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPN-GTDVY-QGVPKDYTGEDVTPQNFLA 115
Query: 120 VLTGRHENAVP-RSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMK 177
VL G E S ++L S H+ +Y T HG L F + E+L DL + + M
Sbjct: 116 VLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMY 174
Query: 178 EKRRFKELMIMVDTCQAATLFNQLQS-PGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT 236
+ + +++++ ++ C++ ++ N L V A ++ E+SY+ + D + D
Sbjct: 175 KHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD--- 231
Query: 237 YYTLAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMET 296
+Y++ + E ++ D + +L + Y + + + Q +
Sbjct: 232 WYSVNWMEDSDVED-LTKETLHKQYHLVKSHTN---TSHVMQYGNKTI------------ 275
Query: 297 IHTDSAYKAFSSKISERKINSEMPFNQLSEHDL-----------KEELENTNIPND 341
S K + +RK +S +P ++ DL K +L NTN +
Sbjct: 276 ----STMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEE 327
>gb|AAF73260.1|AF154349_1 (AF154349) legumain [Rattus norvegicus]
Length = 435
Score = 442 bits (1126), Expect = e-123
Identities = 83/358 (23%), Positives = 148/358 (41%), Gaps = 50/358 (13%)
Query: 2 KILTLVMLLCYSFVSS--TGDTTIHTNNWAVLVCTSRFC--SLHSLVLTFIFSLLGVSRT 57
K+ L+ L+ + D +W V+V S H + +
Sbjct: 4 KVAVLLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQI------ 57
Query: 58 VKRLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENF 117
+ R GIPDE+II+M+ DD+A N N P V N N ++Y V DY G +VT ENF
Sbjct: 58 IHRNGIPDEQIIVMMYDDIANNEENPTPGVVINRPN-GTDVY-KGVPKDYTGEDVTPENF 115
Query: 118 LRVLTGRHENAVP-RSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQ 175
L VL G E S ++L S H+ +Y T HG L F + E+L DL ++
Sbjct: 116 LAVLRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRY 174
Query: 176 MKEKRRFKELMIMVDTCQAATLFNQLQ-SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDR 234
M E + +++++ ++ C++ ++ N L V A ++ E+SY+ + D + + D
Sbjct: 175 MYEHKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEERSTYLGD- 233
Query: 235 FTYYTLAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVM 294
+Y++ + E ++ D + +L + Y + + + Q +
Sbjct: 234 --WYSVNWMEDSDVED-LTKETLHKQYHLVKSHTN---TSHVMQYGNKSI---------- 277
Query: 295 ETIHTDSAYKAFSSKISERKINSEMPFNQLSEHD-----------LKEELENTNIPND 341
S K + + + +S + ++ D LK +L TN +
Sbjct: 278 ------STMKVMQFQGMKHRASSPISLPPVTHLDLTPSPDVPLTILKRKLLRTNNMKE 329
>ref|XP_007399.4| (XM_007399) legumain [Homo sapiens]
Length = 401
Score = 441 bits (1125), Expect = e-123
Identities = 81/356 (22%), Positives = 147/356 (40%), Gaps = 48/356 (13%)
Query: 2 KILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFC--SLHSLVLTFIFSLLGVSRTVK 59
K+ + + D +W V+V S H + + +
Sbjct: 4 KVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQI------IH 57
Query: 60 RLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLR 119
R GIPDE+I++M+ DD+A + N P V N N ++Y V DY G +VT +NFL
Sbjct: 58 RNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPN-GTDVY-QGVPKDYTGEDVTPQNFLA 115
Query: 120 VLTGRHENAVP-RSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMK 177
VL G E S ++L S H+ +Y T HG L F + E+L DL + + M
Sbjct: 116 VLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMY 174
Query: 178 EKRRFKELMIMVDTCQAATLFNQLQS-PGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT 236
+ + +++++ ++ C++ ++ N L V A ++ E+SY+ + D + D
Sbjct: 175 KHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD--- 231
Query: 237 YYTLAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMET 296
+Y++ + E ++ D + +L + Y + + + Q +
Sbjct: 232 WYSVNWMEDSDVED-LTKETLHKQYHLVKSHTN---TSHVMQYGNKTI------------ 275
Query: 297 IHTDSAYKAFSSKISERKINSEMPFNQLSEHDL-----------KEELENTNIPND 341
S K + +RK +S +P ++ DL K +L NTN +
Sbjct: 276 ----STMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEE 327
>ref|NP_035305.1| (NM_011175) protease, cysteine, 1; preprolegumain [Mus musculus]
emb|CAA04439.1| (AJ000990) legumain [Mus musculus]
gb|AAF21659.1|AF044266_1 (AF044266) preprolegumain; cysteine Protease [Mus musculus]
Length = 435
Score = 441 bits (1125), Expect = e-123
Identities = 81/358 (22%), Positives = 149/358 (40%), Gaps = 50/358 (13%)
Query: 2 KILTLVMLLCYSFVSS--TGDTTIHTNNWAVLVCTSRFC--SLHSLVLTFIFSLLGVSRT 57
++ L+ L+ + D +W V+V S H + +
Sbjct: 4 RVAVLLSLVLGAGAVPVGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQI------ 57
Query: 58 VKRLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENF 117
+ R GIPDE+II+M+ DD+A + N P V N N ++Y V DY G +VT ENF
Sbjct: 58 IHRNGIPDEQIIVMMYDDIANSEENPTPGVVINRPN-GTDVY-KGVLKDYTGEDVTPENF 115
Query: 118 LRVLTGRHENAVP-RSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQ 175
L VL G E S ++L S H+ +Y T HG L F + ++L DL ++
Sbjct: 116 LAVLRGDAEAVKGKGSGKVLKSGPRDHVFIYFTDHGATGILVFPN-DDLHVKDLNKTIRY 174
Query: 176 MKEKRRFKELMIMVDTCQAATLFNQLQ-SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDR 234
M E + +++++ ++ C++ ++ N L V A ++ E+SY+ + D + G + D
Sbjct: 175 MYEHKMYQKMVFYIEACESGSMMNHLPDDINVYATTAANPKESSYACYYDEERGTYLGD- 233
Query: 235 FTYYTLAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVM 294
+Y++ + E ++ D + +L + Y + + + Q +
Sbjct: 234 --WYSVNWMEDSDVED-LTKETLHKQYHLVKSHTN---TSHVMQYGNKSI---------- 277
Query: 295 ETIHTDSAYKAFSSKISERKINSEMPFNQLSEHD-----------LKEELENTNIPND 341
S K + + + +S + ++ D LK +L TN +
Sbjct: 278 ------STMKVMQFQGMKHRASSPISLPPVTHLDLTPSPDVPLTILKRKLLRTNDVKE 329
>ref|NP_071562.1| (NM_022226) protease, cysteine, 1 (legumain) [Rattus norvegicus]
dbj|BAA84750.1| (AB032766) legumain [Rattus norvegicus]
Length = 435
Score = 441 bits (1125), Expect = e-123
Identities = 82/358 (22%), Positives = 148/358 (40%), Gaps = 50/358 (13%)
Query: 2 KILTLVMLLCYSFVSS--TGDTTIHTNNWAVLVCTSRFC--SLHSLVLTFIFSLLGVSRT 57
++ L+ L+ + D +W V+V S H + +
Sbjct: 4 RVAVLLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQI------ 57
Query: 58 VKRLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENF 117
+ R GIPDE+II+M+ DD+A N N P V N N ++Y V DY G +VT ENF
Sbjct: 58 IHRNGIPDEQIIVMMYDDIANNEENPTPGVVINRPN-GTDVY-KGVPKDYTGEDVTPENF 115
Query: 118 LRVLTGRHENAVP-RSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQ 175
L VL G E S ++L S H+ +Y T HG L F + E+L DL ++
Sbjct: 116 LAVLRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRY 174
Query: 176 MKEKRRFKELMIMVDTCQAATLFNQLQ-SPGVLAIGSSLKGENSYSHHLDSDIGVSVVDR 234
M E + +++++ ++ C++ ++ N L V A ++ E+SY+ + D + + D
Sbjct: 175 MYEHKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEERSTYLGD- 233
Query: 235 FTYYTLAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVM 294
+Y++ + E ++ D + +L + Y + + + Q +
Sbjct: 234 --WYSVNWMEDSDVED-LTKETLHKQYHLVKSHTN---TSHVMQYGNKSI---------- 277
Query: 295 ETIHTDSAYKAFSSKISERKINSEMPFNQLSEHD-----------LKEELENTNIPND 341
S K + + + +S + ++ D LK +L TN +
Sbjct: 278 ------STMKVMQFQGMKHRASSPISLPPVTHLDLTPSPDVPLTILKRKLLRTNNMKE 329
>ref|NP_005597.1| (NM_005606) legumain; protease, cysteine, 1 (legumain) [Homo
sapiens]
sp|Q99538|LEGU_HUMAN Legumain precursor (Asparaginyl endopeptidase)
emb|CAA70989.1| (Y09862) legumain [Homo sapiens]
gb|AAH08004.1|AAH08004 (BC008004) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 441 bits (1123), Expect = e-122
Identities = 81/356 (22%), Positives = 147/356 (40%), Gaps = 48/356 (13%)
Query: 2 KILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFC--SLHSLVLTFIFSLLGVSRTVK 59
K+ + + D +W V+V S H + + +
Sbjct: 4 KVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQI------IH 57
Query: 60 RLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLR 119
R GIPDE+I++M+ DD+A + N P V N N ++Y V DY G +VT +NFL
Sbjct: 58 RNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPN-GTDVY-QGVPKDYTGEDVTPQNFLA 115
Query: 120 VLTGRHENAVP-RSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMK 177
VL G E S ++L S H+ +Y T HG L F + E+L DL + + M
Sbjct: 116 VLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMY 174
Query: 178 EKRRFKELMIMVDTCQAATLFNQLQS-PGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT 236
+ + +++++ ++ C++ ++ N L V A ++ E+SY+ + D + D
Sbjct: 175 KHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD--- 231
Query: 237 YYTLAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMET 296
+Y++ + E ++ D + +L + Y + + + Q +
Sbjct: 232 WYSVNWMEDSDVED-LTKETLHKQYHLVKSHTN---TSHVMQYGNKTI------------ 275
Query: 297 IHTDSAYKAFSSKISERKINSEMPFNQLSEHDL-----------KEELENTNIPND 341
S K + +RK +S +P ++ DL K +L NTN +
Sbjct: 276 ----STMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEE 327
>gb|AAH03061.1|AAH03061 (BC003061) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 440 bits (1120), Expect = e-122
Identities = 81/356 (22%), Positives = 147/356 (40%), Gaps = 48/356 (13%)
Query: 2 KILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFC--SLHSLVLTFIFSLLGVSRTVK 59
K+ + + D +W V+V S H + + +
Sbjct: 4 KVAVFLSVALGIGAIPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQI------IH 57
Query: 60 RLGIPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLR 119
R GIPDE+I++M+ DD+A + N P V N N ++Y V DY G +VT +NFL
Sbjct: 58 RNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPN-GTDVY-QGVPKDYTGEDVTPQNFLA 115
Query: 120 VLTGRHENAVP-RSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMK 177
VL G E S ++L S H+ +Y T HG L F + E+L DL + + M
Sbjct: 116 VLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMY 174
Query: 178 EKRRFKELMIMVDTCQAATLFNQLQS-PGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFT 236
+ + +++++ ++ C++ ++ N L V A ++ E+SY+ + D + D
Sbjct: 175 KHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD--- 231
Query: 237 YYTLAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMET 296
+Y++ + E ++ D + +L + Y + + + Q +
Sbjct: 232 WYSVNWMEDSDVED-LTKETLHKQYHLVKSHTN---TSHVMQYGNKTI------------ 275
Query: 297 IHTDSAYKAFSSKISERKINSEMPFNQLSEHDL-----------KEELENTNIPND 341
S K + +RK +S +P ++ DL K +L NTN +
Sbjct: 276 ----STMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEE 327
>gb|AAF19550.1|AC007190_18 (AC007190) F23N19.7 [Arabidopsis thaliana]
Length = 536
Score = 439 bits (1119), Expect = e-122
Identities = 57/317 (17%), Positives = 109/317 (33%), Gaps = 36/317 (11%)
Query: 106 DYRGYEVTVENFLRVLTGRHENAVPRSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEEL 164
DY G VT NF VL G + S +++ S HI +Y HGG L + +
Sbjct: 174 DYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHI 233
Query: 165 QSHDLADAVKQMKEKRRFKELMIMVDTCQAATLFNQLQ--SPGVLAIGSSLKGENSYSHH 222
+ D + +K+ +KE++I V+ C++ ++F + + +S E+SY +
Sbjct: 234 YAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTY 293
Query: 223 LDSDIGVSVVDRFT----YYTLAFFERLNIYDNASLNSLFRSYD----PRLLMSTAYYRT 274
+ T Y++A+ E N ++ + Y +T +
Sbjct: 294 CPGMNPSPPSEYITCLGDLYSVAWMEDSET-HNLKKETIKQQYHTVKMRTSNYNTYSGGS 352
Query: 275 DLYQPHLVEVPVTNFFGSVMETIHTD----SAYKAFSS--KISERKIN--------SEMP 320
+ + + + T + S +++R +
Sbjct: 353 HVMEYGNNSIKSEKLYLYQGFDPATVNLPLNELPVKSKIGVVNQRDADLLFLWHMYRTSE 412
Query: 321 FNQLSEHDLKEELENTNIPNDELIAEVTVYTLFPGLSYFGLSTLLRYMNLSRVRVLSMID 380
+ D +EL T L A V + FG + + +NL R L ++D
Sbjct: 413 DGSRKKDDTLKELTETTRHRKHLDASVELIAT----ILFGPT--MNVLNLVREPGLPLVD 466
Query: 381 DVFAFWLVFVLLLDSTN 397
D W ++
Sbjct: 467 D----WECLKSMVRVFE 479
Score = 148 bits (371), Expect = 2e-34
Identities = 20/88 (22%), Positives = 38/88 (42%), Gaps = 10/88 (11%)
Query: 20 DTTIHTNNWAVLVCTSR--FCSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLADDMA 77
D WAVLV S H + + +L ++ G+ +E I++++ DD+A
Sbjct: 44 DEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQIL------RKGGLKEENIVVLMYDDIA 97
Query: 78 CNARNEYPAQVFNNENHKLNLYGDNVEV 105
+ N P + N+ + ++Y V
Sbjct: 98 NHPLNPRPGTLINHPD-GDDVYA-GVPK 123
>dbj|BAB69947.1| (AB060129) legumain [Bos taurus]
Length = 433
Score = 431 bits (1097), Expect = e-119
Identities = 80/353 (22%), Positives = 145/353 (40%), Gaps = 48/353 (13%)
Query: 5 TLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFC--SLHSLVLTFIFSLLGVSRTVKRLG 62
L+ L+ + D +W V+V S H + + V R G
Sbjct: 7 VLLSLVLGTGAVPLEDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQI------VHRNG 60
Query: 63 IPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLT 122
IPDE+II+M+ DD+A + N P V N N ++Y V DY G +VT +NFL VL
Sbjct: 61 IPDEQIIVMMYDDIANSEDNPTPGIVINRPN-GSDVY-QGVLKDYTGEDVTPKNFLAVLR 118
Query: 123 GRHENAVP-RSKRLL-SDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
G E S ++L S H+ +Y T HG L F + E+L DL + ++ M E +
Sbjct: 119 GDAEAVKGVGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNETIRYMYEHK 177
Query: 181 RFKELMIMVDTCQAATLFNQL-QSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYT 239
+++++ ++ C++ ++ N L V A ++ E+SY+ + D + D +Y+
Sbjct: 178 MYQKMVFYIEACESGSMMNHLPPDINVYATTAANPRESSYACYYDEQRSTFLGD---WYS 234
Query: 240 LAFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHT 299
+ + E ++ D + +L + Y + + + Q +
Sbjct: 235 VNWMEDSDVED-LTKETLHKQYQLVKSHTN---TSHVMQYGNKSISAMKL---------- 280
Query: 300 DSAYKAFSSKISERKINSEMPFNQLSEHDL-----------KEELENTNIPND 341
+ + + +S + +S DL K +L +TN +
Sbjct: 281 ------MQFQGLKHQASSPISLPAVSRLDLTPSPEVPLSIMKRKLMSTNDLQE 327
>ref|XP_039644.1| (XM_039644) phosphatidylinositol glycan, class K [Homo sapiens]
gb|AAB81597.1| (AF022913) GPI transamidase [Homo sapiens]
gb|AAH20737.1|AAH20737 (BC020737) Unknown (protein for MGC:22559) [Homo sapiens]
Length = 395
Score = 403 bits (1026), Expect = e-111
Identities = 160/325 (49%), Positives = 214/325 (65%), Gaps = 20/325 (6%)
Query: 22 TIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLADDMACNAR 81
+ HTNNWAVLVCTSRF + + L V R+VKRLGIPD I+LMLADDMACN R
Sbjct: 40 SGHTNNWAVLVCTSRFW----FNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPR 95
Query: 82 NEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRLLSDEGS 141
N PA VF+++N +LN+YGD+VEVDYR YEVTVENFLRVLTGR + PRSKRLLSD+ S
Sbjct: 96 NPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPSTPRSKRLLSDDRS 155
Query: 142 HILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAATLFNQL 201
+IL+YMTGHGG+ FLKFQD+EE+ + +LADA +QM +KRR+ EL+ ++DTCQ A+++ +
Sbjct: 156 NILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERF 215
Query: 202 QSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFERLNIYDNASLNSLFRSY 261
SP ++A+ SS GE+S SH D IGV ++DR+T+Y L F E +N ++N LF+
Sbjct: 216 YSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVC 275
Query: 262 DPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVM------ETIHTDSAYKAFSSKISERKI 315
L +ST +RTDL+Q V +T+FFGSV ETI + S E ++
Sbjct: 276 PKSLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQDSEIMESSYKEDQM 335
Query: 316 NSE----------MPFNQLSEHDLK 330
+ + +P Q+ K
Sbjct: 336 DEKLMEPLKYAEQLPVAQIIHQKPK 360
>emb|CAA68871.1| (Y07596) gpi8 [Homo sapiens]
Length = 396
Score = 403 bits (1026), Expect = e-111
Identities = 160/325 (49%), Positives = 214/325 (65%), Gaps = 20/325 (6%)
Query: 22 TIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLADDMACNAR 81
+ HTNNWAVLVCTSRF + + L V R+VKRLGIPD I+LMLADDMACN R
Sbjct: 41 SGHTNNWAVLVCTSRFW----FNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPR 96
Query: 82 NEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRLLSDEGS 141
N PA VF+++N +LN+YGD+VEVDYR YEVTVENFLRVLTGR + PRSKRLLSD+ S
Sbjct: 97 NPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPSTPRSKRLLSDDRS 156
Query: 142 HILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAATLFNQL 201
+IL+YMTGHGG+ FLKFQD+EE+ + +LADA +QM +KRR+ EL+ ++DTCQ A+++ +
Sbjct: 157 NILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERF 216
Query: 202 QSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFERLNIYDNASLNSLFRSY 261
SP ++A+ SS GE+S SH D IGV ++DR+T+Y L F E +N ++N LF+
Sbjct: 217 YSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVC 276
Query: 262 DPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVM------ETIHTDSAYKAFSSKISERKI 315
L +ST +RTDL+Q V +T+FFGSV ETI + S E ++
Sbjct: 277 PKSLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQDSEIMESSYKEDQM 336
Query: 316 NSE----------MPFNQLSEHDLK 330
+ + +P Q+ K
Sbjct: 337 DEKLMEPLKYAEQLPVAQIIHQKPK 361
CPU time: 120.10 user secs. 2.66 sys. secs 122.76 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.312 0.178 0.585
Gapped
Lambda K H
0.270 0.0624 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 331136381
Number of Sequences: 887402
Number of extensions: 19536565
Number of successful extensions: 32084
Number of sequences better than 10.0: 89
Number of HSP's better than 10.0 without gapping: 72
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 31378
Number of HSP's gapped (non-prelim): 93
length of query: 428
length of database: 277,845,442
effective HSP length: 58
effective length of query: 370
effective length of database: 226,376,126
effective search space: 83759166620
effective search space used: 83759166620
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.0 bits)
S2: 75 (33.2 bits)
