Sequences with E-value BETTER than threshold (0.002) 490 e-137
485 e-136
481 e-135
480 e-134
477 e-133
477 e-133
476 e-133
475 e-133
475 e-133
473 e-132
473 e-132
472 e-132
469 e-131
469 e-131
469 e-131
466 e-130
463 e-129
463 e-129
462 e-129
462 e-129
462 e-129
461 e-129
461 e-129
461 e-129
460 e-128
460 e-128
459 e-128
457 e-128
457 e-127
456 e-127
455 e-127
455 e-127
450 e-125
439 e-122
439 e-122
427 e-118
427 e-118
427 e-118
424 e-118
423 e-117
423 e-117
422 e-117
422 e-117
422 e-117
418 e-116
416 e-115
413 e-114
413 e-114
406 e-112
401 e-110
Alignments
>emb|CAB16318.1| (Z99174) cysteine proteinase precursor [Vicia narbonensis]
Length = 488
Score = 490 bits (1250), Expect = e-137
Identities = 70/331 (21%), Positives = 124/331 (37%), Gaps = 35/331 (10%)
Query: 4 TAIHNSAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLA 63
+++H S P R R ++ WAVLV S + NYRH ++V
Sbjct: 16 SSLHGSVAARPNRLEWEPVIRLPGEPVDADVEDEMGTRWAVLVAGSNGYGNYRHQADVCH 75
Query: 64 LYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTV 123
Y + + G+ + NI++ + +D+ N NPRPG + G N+Y V DY G+ VT
Sbjct: 76 AYQLLIKGGVKEENIVVFMYDDIAYNEMNPRPGVIINHPQGPNVY-DGVPKDYNGDFVTA 134
Query: 124 ESFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAI 182
E+F V+ G S +++ + + + IY + HGG + + + +D +
Sbjct: 135 ENFYAVILGDKSKVRGGSGKVINSKAEDRIFIYCSDHGGPGVLGMPNMPYVYAMDFIDVL 194
Query: 183 QTMFEDNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------D 233
+ Y +M++ ++C S S++E I NV ++S E S+
Sbjct: 195 KKKHASGGYKKMVIYVEACESGSIFEGIMPKDINVYVTTASNAQENSWGTYCPGVDPAPP 254
Query: 234 TDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLSN---TGVRKDHYP 289
+ + D Y+V ++ E L Q Y+ N +
Sbjct: 255 PEYITCLGD---LYSVAWMEDSETHNLKRETVKQQYMSVRERTSNYKNYPLGSHVMQYG- 310
Query: 290 KDVKRVRVTDFFGSSRIFQHLSEEIVLDDEF 320
E++ L F
Sbjct: 311 ----------------DTNITDEKLYLYHGF 325
>sp|P49045|VPE_SOYBN VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||T07132 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - soybean
dbj|BAA06030.1| (D28876) cysteine proteinase [Glycine max]
Length = 495
Score = 485 bits (1236), Expect = e-136
Identities = 68/329 (20%), Positives = 120/329 (35%), Gaps = 37/329 (11%)
Query: 6 IHNSAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALY 65
+H A P R + WAVLV S + NYRH ++V Y
Sbjct: 27 VHG-AAARPNRKEWDSVIKLPTEPV-DADSDEVGTRWAVLVAGSNGYGNYRHQADVCHAY 84
Query: 66 HSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVES 125
+ + G+ + NI++ + +D+ N NPR G + G +LY V DY G+ VT E+
Sbjct: 85 QLLIKGGLKEENIVVFMYDDIATNELNPRHGVIINHPEGEDLYA-GVPKDYTGDNVTTEN 143
Query: 126 FIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQT 184
V+ G S +++ + + + IY + HGG + + L +D ++
Sbjct: 144 LFAVILGDKSKLKGGSGKVINSKPEDRIFIYYSDHGGPGILGMPNMPYLYAMDFIDVLKK 203
Query: 185 MFEDNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTD 235
Y EM++ ++C S S++E I N+ ++S E S+ +
Sbjct: 204 KHASGSYKEMVIYVEACESGSVFEGIMPKDLNIYVTTASNAQENSWGTYCPGMDPSPPPE 263
Query: 236 IGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLSN---TGVRKDHYPKD 291
+ D Y+V ++ E L + Q Y +N +
Sbjct: 264 YITCLGD---LYSVAWMEDSEAHNLKRESVKQQYKSVKQRTSNFNNYAMGSHVMQYG--- 317
Query: 292 VKRVRVTDFFGSSRIFQHLSEEIVLDDEF 320
+E++ L F
Sbjct: 318 --------------DTNITAEKLYLYQGF 332
>emb|CAC18099.1| (AJ251453) putative legumain [Zea mays]
Length = 486
Score = 481 bits (1227), Expect = e-135
Identities = 66/327 (20%), Positives = 126/327 (38%), Gaps = 28/327 (8%)
Query: 9 SAQFPPLRFRIFFGFRFERSSGLSEKVT---GHTNNWAVLVCTSKFWFNYRHVSNVLALY 65
SA P R+ R + T WAVL+ S ++NYRH +++ Y
Sbjct: 18 SAWARP---RLEPAIRLPSQRAAAADETDDGDVGTRWAVLIAGSSGYYNYRHQADICHAY 74
Query: 66 HSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVES 125
+K+ G+ D NII+ + +D+ + NPRPG + G ++Y V DY G +V V++
Sbjct: 75 QIMKKGGLKDENIIVFMYDDIAHSPENPRPGVIINHPQGGDVYA-GVPKDYTGRDVNVDN 133
Query: 126 FIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQT 184
F VL G S +++ + ++ ++ + HGG + L DL ++
Sbjct: 134 FFAVLLGNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKK 193
Query: 185 MFEDNRYHEMLVIADSCRSASMYEWID--SPNVLSLSSSLTHEESYSYDV-------DTD 235
Y ++ ++C S S++E + NV + ++S E S+ +
Sbjct: 194 KHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYATTASNADESSWGTYCPGEFPSPPPE 253
Query: 236 IGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCL---SNTGVRKDHYPKD 291
+ D Y+V ++ + L + + Q Y S + +
Sbjct: 254 YDTCLGD---LYSVAWMEDSDFHNLRTESLKQQYKLVKDRTAVHDTFSYGSHVMQYGALE 310
Query: 292 VKRVRVTDFFG----SSRIFQHLSEEI 314
+ R+ + G + +
Sbjct: 311 LNVQRLFSYIGTDPANDGNTFIEDNSL 337
>emb|CAB64544.1| (AJ131718) legumain-like protease [Zea mays]
Length = 486
Score = 480 bits (1224), Expect = e-134
Identities = 65/327 (19%), Positives = 125/327 (37%), Gaps = 28/327 (8%)
Query: 9 SAQFPPLRFRIFFGFRFERSSGLSEKVTG---HTNNWAVLVCTSKFWFNYRHVSNVLALY 65
SA P R+ R + T WAVL+ S ++NYRH +++ Y
Sbjct: 18 SAWARP---RLETAIRLPSQRAAAADETDDGAVGTRWAVLIAGSSGYYNYRHQADICHAY 74
Query: 66 HSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVES 125
+K+ G+ D NII+ + +D+ + NPRPG + G ++Y V DY G +V V++
Sbjct: 75 QIMKKGGLKDENIIVFMYDDIAHSPENPRPGVIINHPQGGDVYA-GVPKDYTGRDVNVDN 133
Query: 126 FIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQT 184
F VL G S +++ + ++ ++ + HGG + L DL ++
Sbjct: 134 FFAVLLGNKTALRGGSGKVVDSGPDDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKK 193
Query: 185 MFEDNRYHEMLVIADSCRSASMYEWID--SPNVLSLSSSLTHEESYSYDV-------DTD 235
Y ++ ++C S S++E + NV + ++S E S+ +
Sbjct: 194 KHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPE 253
Query: 236 IGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCL---SNTGVRKDHYPKD 291
+ D Y+V ++ + L + + Q Y S + +
Sbjct: 254 YDTCLGD---LYSVAWMEDSDFHNLRTESLKQQYKLVKDRTAVHDTFSYGSHVMQYGALE 310
Query: 292 VKRVRVTDFFG----SSRIFQHLSEEI 314
+ + + G + +
Sbjct: 311 LNVQHLFSYIGTDPANDGNTFIEDNSL 337
>emb|CAB64545.1| (AJ131719) legumain-like protease [Zea mays]
Length = 481
Score = 477 bits (1217), Expect = e-133
Identities = 63/308 (20%), Positives = 122/308 (39%), Gaps = 23/308 (7%)
Query: 9 SAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSI 68
SA P R+ R ++ WAVL+ S ++NYRH +++ Y +
Sbjct: 18 SAWARP---RLEPTIRLPSDRAAADD--AVGTRWAVLIAGSNGYYNYRHQADICHAYQIM 72
Query: 69 KRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIR 128
K+ G+ D NI++ + +D+ + NPRPG + G ++Y V DY G +V V++F
Sbjct: 73 KKGGLKDENIVVFMYDDIAHSPENPRPGVIINHPQGGDVYA-GVPKDYTGRDVNVDNFFA 131
Query: 129 VLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFE 187
VL G S +++ + ++ ++ + HGG + L DL ++
Sbjct: 132 VLLGNKTALRGGSGKVVDSGPNDHISVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHA 191
Query: 188 DNRYHEMLVIADSCRSASMYEWID--SPNVLSLSSSLTHEESYSYDV-------DTDIGV 238
Y ++ ++C S S++E + NV + ++S E S+ +
Sbjct: 192 AGTYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDT 251
Query: 239 YVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKC---LSNTGVRKDHYPKDVKR 294
+ D Y+V ++ + L + + Q Y S + +
Sbjct: 252 CLGD---LYSVAWMEDSDFHNLRTESLKQQYNLVKDRTAVQDTFSYGSHVMQYGSLGLNV 308
Query: 295 VRVTDFFG 302
+ + G
Sbjct: 309 KHLFSYIG 316
>sp|P49043|VPE_CITSI VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||S51117 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - sweet
orange
emb|CAA87720.1| (Z47793) cystein proteinase (by similarity) [Citrus sinensis]
prf||2208463A vascular processing protease [Citrus sinensis]
Length = 494
Score = 477 bits (1216), Expect = e-133
Identities = 66/316 (20%), Positives = 130/316 (40%), Gaps = 25/316 (7%)
Query: 3 STAIHNSAQFPPLRFRIFFGFRFERSSG----LSEKVTGHTNNWAVLVCTSKFWFNYRHV 58
S I P +RF + G +++ WAVL+ S ++NYRH
Sbjct: 23 SRDIAGDILKLPSEA-----YRFFHNGGGGAKVNDDDDSVGTRWAVLLAGSNGFWNYRHQ 77
Query: 59 SNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRG 118
+++ Y +++ G+ D NII+ + +D+ N NPRPG + G ++Y V DY G
Sbjct: 78 ADICHAYQLLRKGGLKDENIIVFMYDDIAFNEENPRPGVIINHPHGDDVY-KGVPKDYTG 136
Query: 119 EEVTVESFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVD 177
E+VTVE F V+ G T S +++ + ++ I+ + HGG + S + +
Sbjct: 137 EDVTVEKFFAVVLGNKTALTGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSRYIYADE 196
Query: 178 LAYAIQTMFEDNRYHEMLVIADSCRSASMYEW--IDSPNVLSLSSSLTHEESYSYDV--- 232
L ++ Y ++ ++C S S++E ++ N+ + ++S E S+
Sbjct: 197 LIDVLKKKHASGNYKSLVFYLEACESGSIFEGLLLEGLNIYATTASNAEESSWGTYCPGE 256
Query: 233 ----DTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCL-SNTGVRKD 286
+ + D Y++ ++ ++ L + Q Y S
Sbjct: 257 IPGPPPEYSTCLGD---LYSIAWMEDSDIHNLRTETLHQQYELVKTRTASYNSYGSHVMQ 313
Query: 287 HYPKDVKRVRVTDFFG 302
+ + + + + G
Sbjct: 314 YGDIGLSKNNLFTYLG 329
>gb|AAF89646.1|AF169019_1 (AF169019) seed maturation protein PM40 [Glycine max]
Length = 496
Score = 476 bits (1214), Expect = e-133
Identities = 61/326 (18%), Positives = 119/326 (35%), Gaps = 36/326 (11%)
Query: 10 AQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIK 69
A R + ++ WAVLV S + NYRH ++V Y +
Sbjct: 29 AAARANRKEWDSVIKLPAEPVDADSDHEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLI 88
Query: 70 RLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRV 129
+ G+ + NI++ + +D+ + NPRPG + G ++Y V DY GE VT ++ V
Sbjct: 89 KGGLKEENIVVFMYDDIATDELNPRPGVIINHPEGQDVYA-GVPKDYTGENVTAQNLFAV 147
Query: 130 LTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFED 188
+ G + S +++ + + + IY + HGG + + L +D ++
Sbjct: 148 ILGDKNKVKGGSGKVINSKPEDRIFIYYSDHGGPGVLGMPNMPYLYAMDFIEVLKKKHAS 207
Query: 189 NRYHEMLVIADSCRSAS-MYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGV 238
Y +M++ ++C S + + + I + ++S E S+ +
Sbjct: 208 GGYKKMVIYVEACESGNHVLKGIMPKDLQIYVTTASNAQENSWGTYCPGMDPSPPPEYIT 267
Query: 239 YVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLSN---TGVRKDHYPKDVKR 294
+ D Y+V ++ E L + Q Y +N +
Sbjct: 268 CLGD---LYSVAWMEDSETHNLKRESVKQQYKSVKQRTSNFNNYAMGSHVMQYG------ 318
Query: 295 VRVTDFFGSSRIFQHLSEEIVLDDEF 320
+E++ L F
Sbjct: 319 -----------DTNITAEKLYLYQGF 333
>pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring vetch
emb|CAA07639.1| (AJ007743) cysteine proteinase precursor [Vicia sativa]
Length = 503
Score = 475 bits (1211), Expect = e-133
Identities = 64/332 (19%), Positives = 125/332 (37%), Gaps = 37/332 (11%)
Query: 4 TAIHNSAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLA 63
+++H S P R R ++ WAVLV S + NYRH ++V
Sbjct: 31 SSLHGSV-ARPNRLEWEPVIRLPGEPVDADVEDEIGTRWAVLVAGSNGYGNYRHQADVCH 89
Query: 64 LYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTV 123
Y + + G+ + NI++ + +D+ + NPRPG + G N+Y V DY G+ VT
Sbjct: 90 AYQLLIKGGVKEENIVVFMYDDIAYSEFNPRPGVIINHPQGPNVY-DGVPKDYTGDFVTA 148
Query: 124 ESFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAI 182
++ V+ G S +++ + + + IY + HGG + + + +D +
Sbjct: 149 DNLYAVILGDKSKVRGGSGKVINSKAEDRIFIYYSDHGGPGVLGMPNMPYVYAMDFIDVL 208
Query: 183 QTMFEDNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------- 232
+ Y +M++ ++C S S+++ I +V ++S E S+ +
Sbjct: 209 KKKHASRGYQQMVIYVEACESGSVFQGIMPKDIDVYVTTASNAEESSWGTYLVPGVYPAS 268
Query: 233 DTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLSN---TGVRKDHY 288
+ + D Y+V ++ E L Q + +N ++
Sbjct: 269 PPEYITCLGD---LYSVAWMEDSETHNLKRETLKQQFASVKERTLNNNNYGLGSHVTEYG 325
Query: 289 PKDVKRVRVTDFFGSSRIFQHLSEEIVLDDEF 320
E++ L F
Sbjct: 326 -----------------DTNITDEKLYLYHGF 340
>sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptidase)
pir||JX0344 legumain (EC 3.4.22.34) precursor - jack bean
dbj|BAA06596.1| (D31787) asparaginyl endopeptidase (Legumain) [Canavalia
ensiformis]
Length = 475
Score = 475 bits (1210), Expect = e-133
Identities = 66/330 (20%), Positives = 127/330 (38%), Gaps = 40/330 (12%)
Query: 5 AIHNSAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLAL 64
++H +A R + E WAVLV S + NYRH ++V
Sbjct: 9 SLHGTA-ARLNRREWDSVIQLPTEPVDDE----VGTRWAVLVAGSNGYGNYRHQADVCHA 63
Query: 65 YHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVE 124
Y + + G+ + NI++ + +D+ N+ NPRPG + G ++Y V DY GE+VT E
Sbjct: 64 YQLLIKGGVKEENIVVFMYDDIAYNAMNPRPGVIINHPQGPDVYA-GVPKDYTGEDVTPE 122
Query: 125 SFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQ 183
+ V+ G S +++ ++ + + I+ + HGG + ++ + +D ++
Sbjct: 123 NLYAVILGDKSKVKGGSGKVINSNPEDRIFIFYSDHGGPGVLGMPNAPFVYAMDFIDVLK 182
Query: 184 TMFEDNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DT 234
Y EM++ ++C S S++E I N+ ++S E S+
Sbjct: 183 KKHASGGYKEMVIYIEACESGSIFEGIMPKDLNIYVTTASNAQENSFGTYCPGMNPPPPE 242
Query: 235 DIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLSN---TGVRKDHYPK 290
+ + D Y+V+++ E L Q Y ++ +
Sbjct: 243 EYVTCLGD---LYSVSWMEDSETHNLKRETVQQQYQSVRKRTSNSNSYRFGSHVMQYG-- 297
Query: 291 DVKRVRVTDFFGSSRIFQHLSEEIVLDDEF 320
+E++ L F
Sbjct: 298 ---------------DTNITAEKLYLYHGF 312
>gb|AAD04883.1| (AF082347) C13 endopeptidase NP1 precursor [Zea mays]
Length = 485
Score = 473 bits (1207), Expect = e-132
Identities = 63/310 (20%), Positives = 121/310 (38%), Gaps = 23/310 (7%)
Query: 9 SAQFPPLRFRIFFGFRFERSS--GLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYH 66
SA P R+ R WAVL+ S ++NYRH +++ Y
Sbjct: 18 SAWARP---RLEPTIRLPSERAAADETDDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQ 74
Query: 67 SIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESF 126
+K+ G+ D NI++ + +D+ + NPRPG + G ++Y V DY G +V V++F
Sbjct: 75 IMKKGGLKDENIVVFMYDDIAHSPENPRPGVIINHPQGGDVYA-GVPKDYTGRDVNVDNF 133
Query: 127 IRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTM 185
VL G S +++ + ++ ++ + HGG + L DL ++
Sbjct: 134 FAVLLGNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKK 193
Query: 186 FEDNRYHEMLVIADSCRSASMYEWID--SPNVLSLSSSLTHEESYSYDV-------DTDI 236
Y ++ ++C S S++E + NV + ++S E S+ +
Sbjct: 194 HAAGTYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEY 253
Query: 237 GVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKC---LSNTGVRKDHYPKDV 292
+ D Y+V ++ + L + + Q Y S + ++
Sbjct: 254 DTCLGD---LYSVAWMEDSDFHNLRTESLKQQYNLVKDRTAVQDTFSYGSHVMQYGSLEL 310
Query: 293 KRVRVTDFFG 302
+ + G
Sbjct: 311 NVKHLFSYIG 320
>gb|AAF69014.1|AF260827_1 (AF260827) cysteine protease [Ipomoea batatas]
Length = 492
Score = 473 bits (1207), Expect = e-132
Identities = 65/321 (20%), Positives = 128/321 (39%), Gaps = 22/321 (6%)
Query: 12 FPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRL 71
P R FF E + + WAVL+ S ++NYRH +++ Y +K
Sbjct: 27 KLPSEARRFFRPAEEENREADGDDS-VGTRWAVLIAGSNGYWNYRHQADICHAYQILKAG 85
Query: 72 GIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLT 131
G+ D NI++ + +D+ N NPR G + + G ++Y V DY G++VT + + V+
Sbjct: 86 GLKDENIVVFMYDDIAYNEENPRKGIIINSPHGEDVYH-GVPKDYTGDDVTANNLLAVIL 144
Query: 132 GRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNR 190
G S +++ + ++ IY + HGG + S L +L A++
Sbjct: 145 GDKSAVKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPYLYADELNAALKKKHAAGA 204
Query: 191 YHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGVYVI 241
Y ++ ++C S S++E I N+ + ++S E S+ + +
Sbjct: 205 YKSLVFYLEACESGSIFEGILPKDINIYATTASNAIESSWGTYCPGEYPSPPPEYETCLG 264
Query: 242 DRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLS--NTGVRKDHYPKDVKRVRVT 298
D Y++ ++ ++ L + + Q Y + + ++ +
Sbjct: 265 D---LYSIAWMEDSDIHNLRTESLKQQYNLVKDRTLNGNTAYGSHVMQYGDLELNADSLF 321
Query: 299 DFFGSSRIFQHLSEEIVLDDE 319
+ G +E DE
Sbjct: 322 MYMG----TNPANENFTFVDE 338
>emb|CAC18100.1| (AJ251454) putative legumain [Zea mays]
Length = 485
Score = 472 bits (1203), Expect = e-132
Identities = 64/310 (20%), Positives = 121/310 (38%), Gaps = 23/310 (7%)
Query: 9 SAQFPPLRFRIFFGFRFERSS--GLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYH 66
SA P R+ R WAVL+ S ++NYRH +++ Y
Sbjct: 18 SAWARP---RLEPTIRLPSERAAADETDDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQ 74
Query: 67 SIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESF 126
+K+ G+ D NI++ + +D+ + NPRPG + G ++Y V DY G EV V++F
Sbjct: 75 IMKKGGLKDENIVVFMYDDIAHSPENPRPGVIINHPQGGDVYA-GVPKDYTGREVNVDNF 133
Query: 127 IRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTM 185
VL G S +++ + ++ ++ + HGG + L DL ++
Sbjct: 134 FAVLLGNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKK 193
Query: 186 FEDNRYHEMLVIADSCRSASMYEWID--SPNVLSLSSSLTHEESYSYDV-------DTDI 236
Y ++ ++C S S++E + NV + ++S E S+ +
Sbjct: 194 HAAGTYKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEY 253
Query: 237 GVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKC---LSNTGVRKDHYPKDV 292
+ D Y+V ++ + L + + Q Y S + ++
Sbjct: 254 DTCLGD---LYSVAWMEDSDFHNLRTESLKQQYNLVKDRTAVQDTFSYGSHVMQYGSLEL 310
Query: 293 KRVRVTDFFG 302
+ + G
Sbjct: 311 NVKHLFSYIG 320
>ref|XP_039644.1| (XM_039644) phosphatidylinositol glycan, class K [Homo sapiens]
gb|AAB81597.1| (AF022913) GPI transamidase [Homo sapiens]
gb|AAH20737.1|AAH20737 (BC020737) Unknown (protein for MGC:22559) [Homo sapiens]
Length = 395
Score = 469 bits (1196), Expect = e-131
Identities = 159/285 (55%), Positives = 212/285 (73%), Gaps = 3/285 (1%)
Query: 36 TGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRP 95
+GHTNNWAVLVCTS+FWFNYRHV+N L++Y S+KRLGIPDS+I++MLA+D+ CN RNP+P
Sbjct: 40 SGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPRNPKP 99
Query: 96 GTVYAARA-GTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKRLLTDHQSNVLI 154
TV++ + N+YG DVEVDYR EVTVE+F+RVLTGR P+TPRSKRLL+D +SN+LI
Sbjct: 100 ATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPSTPRSKRLLSDDRSNILI 159
Query: 155 YLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWIDSPN 214
Y+TGHGG+ F+KFQDSEE+TN++LA A + M++ RY+E+L I D+C+ ASMYE SPN
Sbjct: 160 YMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPN 219
Query: 215 VLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTKEVKALNSSANMQDYIDSCPA 274
+++L+SS E+S S+ D IGV+++DRYT Y + FL + A S NM D CP
Sbjct: 220 IMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPA--SQTNMNDLFQVCPK 277
Query: 275 RKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLSEEIVLDDE 319
C+S G R D + +D K V +TDFFGS R + +E I L +
Sbjct: 278 SLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQD 322
>gb|AAF89679.1| (AF169973) asparaginyl endopeptidase [Sesamum indicum]
Length = 489
Score = 469 bits (1196), Expect = e-131
Identities = 66/304 (21%), Positives = 120/304 (38%), Gaps = 21/304 (6%)
Query: 19 IFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNI 78
R+ + + WAVLV S + NYRH ++V Y +K+ G+ D NI
Sbjct: 35 WDPIIRWPLDR---RETEDNATRWAVLVAGSNGFGNYRHQADVCHAYQILKKGGLRDENI 91
Query: 79 IMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPAT 138
I+ + +D+ N NPR G + G ++Y V DY GE+VT E+ V+ G
Sbjct: 92 IVFMYDDIAMNELNPRKGVIINHPTGGDVYA-GVPKDYTGEQVTAENLYAVILGDKSAIK 150
Query: 139 PRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVI 197
S +++ + + IY + HGG + + L D ++ Y EM++
Sbjct: 151 GGSGKVVDSKPNDRIFIYYSDHGGPGVLGMPNMPYLYANDFIEVLKKKHASGTYKEMVIY 210
Query: 198 ADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDVD-------TDIGVYVIDRYTHYT 248
++C S S++E + D ++ ++S E S+ + + D Y+
Sbjct: 211 VEACESGSVFEGLMPDDLDIYVTTASNAEESSWGTYCPGMDPPPPPEYITCLGD---LYS 267
Query: 249 VNFLT-KEVKALNSSANMQDYIDSCPARKCLSN---TGVRKDHYPKDVKRVRVTDFFGSS 304
V ++ E L Q Y + ++ K +K ++ + G
Sbjct: 268 VAWMEDSESHNLKRETVEQQYQQVKERTSNFNTYNAGSHVMEYGNKSIKSEKLYLYQGFD 327
Query: 305 RIFQ 308
+
Sbjct: 328 PATE 331
>emb|CAA68871.1| (Y07596) gpi8 [Homo sapiens]
Length = 396
Score = 469 bits (1195), Expect = e-131
Identities = 159/285 (55%), Positives = 212/285 (73%), Gaps = 3/285 (1%)
Query: 36 TGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRP 95
+GHTNNWAVLVCTS+FWFNYRHV+N L++Y S+KRLGIPDS+I++MLA+D+ CN RNP+P
Sbjct: 41 SGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPRNPKP 100
Query: 96 GTVYAARA-GTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKRLLTDHQSNVLI 154
TV++ + N+YG DVEVDYR EVTVE+F+RVLTGR P+TPRSKRLL+D +SN+LI
Sbjct: 101 ATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPSTPRSKRLLSDDRSNILI 160
Query: 155 YLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWIDSPN 214
Y+TGHGG+ F+KFQDSEE+TN++LA A + M++ RY+E+L I D+C+ ASMYE SPN
Sbjct: 161 YMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPN 220
Query: 215 VLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTKEVKALNSSANMQDYIDSCPA 274
+++L+SS E+S S+ D IGV+++DRYT Y + FL + A S NM D CP
Sbjct: 221 IMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPA--SQTNMNDLFQVCPK 278
Query: 275 RKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLSEEIVLDDE 319
C+S G R D + +D K V +TDFFGS R + +E I L +
Sbjct: 279 SLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQD 323
>sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE)
pir||JQ2387 vacuolar processing enzyme (EC 3.4.22.-) precursor - castor bean
dbj|BAA04225.1| (D17401) precursor of vacuolar processing enzyme [Ricinus communis]
Length = 497
Score = 466 bits (1187), Expect = e-130
Identities = 65/312 (20%), Positives = 118/312 (36%), Gaps = 19/312 (6%)
Query: 6 IHNSAQFPPLRFRIFFG-FRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLAL 64
I F E + WAVLV S + NYRH ++V
Sbjct: 26 IPGLLASRLNPFEPGILMPTEEAEPVQVDDDDQLGTRWAVLVAGSMGFGNYRHQADVCHA 85
Query: 65 YHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVE 124
Y +++ G+ + NII+ + +D+ N NPRPG + G ++Y V DY GE VT +
Sbjct: 86 YQLLRKGGLKEENIIVFMYDDIAKNELNPRPGVIINHPQGEDVYA-GVPKDYTGEHVTAK 144
Query: 125 SFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQ 183
+ VL G S +++ + + +Y + HGG + + L +D ++
Sbjct: 145 NLYAVLLGDKSAVQGGSGKVVDSKPNDRIFLYYSDHGGPGVLGMPNLPYLYAMDFIEVLK 204
Query: 184 TMFEDNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DT 234
Y +M++ ++C S S++E I ++ ++S E S+
Sbjct: 205 KKHAAGGYKKMVIYVEACESGSIFEGIMPKDVDIYVTTASNAQESSWGTYCPGMEPSPPP 264
Query: 235 DIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLSN---TGVRKDHYPK 290
+ + D Y+V ++ E L Q Y + + +
Sbjct: 265 EFTTCLGD---LYSVAWMEDSESHNLKKETVKQQYSSVKARTSNYNTYAAGSHVMQYGNQ 321
Query: 291 DVKRVRVTDFFG 302
+K ++ F G
Sbjct: 322 SIKADKLYLFQG 333
>dbj|BAA76744.1| (D89971) asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
Length = 483
Score = 463 bits (1180), Expect = e-129
Identities = 61/292 (20%), Positives = 121/292 (40%), Gaps = 19/292 (6%)
Query: 24 RFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLA 83
RF ++ E WAVL+ S ++NYRH S+V Y + + G+ + NI++ +
Sbjct: 33 RFFQAPATDENDE--GTRWAVLIAGSNGYWNYRHQSDVCHAYQLLTKGGLKEENIVVFMY 90
Query: 84 EDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKR 143
+D+ N NPRPG + + G ++Y V DY GE+VTV +F + G T S +
Sbjct: 91 DDIAFNEENPRPGVIINSPHGNDVY-KGVPKDYVGEDVTVNNFFAAILGNKSALTGGSGK 149
Query: 144 LL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCR 202
++ + ++ IY + HGG + S + DL ++ Y + + C
Sbjct: 150 VVNSGPNDHIFIYYSDHGGPGVLGMPTSPYMYASDLIEVLKKKHASGTYKSLAFYLEGCE 209
Query: 203 SASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGVYVIDRYTHYTVNFLT 253
S S++ + + N+ + +++ E S+ + + D Y+V ++
Sbjct: 210 SGSIFGGLLPEGLNIYATTAANAEESSWGTYCPGDNPSPPPEYETCLGD---LYSVAWME 266
Query: 254 -KEVKALNSSANMQDYIDSCPARKCLS--NTGVRKDHYPKDVKRVRVTDFFG 302
++ L + Q + + + + + V+ + G
Sbjct: 267 DSDIHNLRTETLHQQFELVKQRTMNGNSAYGSHVMQYGDVGLSKNNVSLYLG 318
>sp|P49044|VPE_VICSA VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) (PROTEINASE B)
pir||S49175 legumain (EC 3.4.22.34) precursor [similarity] - spring vetch
pir||S68984 cysteine proteinase precursor - spring vetch
emb|CAA84383.1| (Z34899) cysteine proteinase [Vicia sativa]
Length = 493
Score = 463 bits (1180), Expect = e-129
Identities = 66/310 (21%), Positives = 122/310 (39%), Gaps = 22/310 (7%)
Query: 4 TAIHNSAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLA 63
+ P RF R + WA+L+ S ++NYRH S+V
Sbjct: 26 RDLPGDYLRLPSETS-----RFFREPKNDDDFE--GTRWAILLAGSNGYWNYRHQSDVCH 78
Query: 64 LYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTV 123
Y +++ G + NII+ + +D+ N NPRPG + G ++Y V DY G EV
Sbjct: 79 AYQLLRKGGSKEENIIVFMYDDIASNEENPRPGVIINKPDGDDVYA-GVPKDYTGAEVHA 137
Query: 124 ESFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAI 182
++F L G T S +++ + ++ +Y T HGG + L DL +
Sbjct: 138 DNFYAALLGNKSALTGGSGKVVDSGPNDHIFVYYTDHGGPGVLGMPVGPYLYASDLNEVL 197
Query: 183 QTMFEDNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDVD------- 233
+ Y ++ ++C S S++E + D N+ + ++S E S+ Y
Sbjct: 198 KKKHASGTYKSLVFYLEACESGSIFEGLLPDDLNIYATTASNAEESSWGYYCPGDKPPPP 257
Query: 234 TDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLSNTGVRKDHYPKDV 292
+ + D Y++ ++ EV L + + Q Y ++ +
Sbjct: 258 PEYSTCLGD---LYSIAWMEDSEVHNLQTESLQQQYKLVKNRTISEPYGSHVMEYGDIGL 314
Query: 293 KRVRVTDFFG 302
+ + + G
Sbjct: 315 SKNDLYQYLG 324
>ref|NP_502076.1| (NM_069675) haemoglobinase like [Caenorhabditis elegans]
sp|P49048|YJ96_CAEEL Hypothetical 36.9 kDa protein T05E11.6 in chromosome IV
pir||T24525 hypothetical protein T05E11.6 - Caenorhabditis elegans
emb|CAA92977.1| (Z68751) Similarity to Schistosoma Heamoglobinase (SW:HGLB_SCHMA),
contains similarity to Pfam domain: PF01650 (Peptidase
C13 family), Score=620.4, E-value=3.3e-183, N=1
[Caenorhabditis elegans]
Length = 322
Score = 462 bits (1178), Expect = e-129
Identities = 322/322 (100%), Positives = 322/322 (100%)
Query: 1 MRSTAIHNSAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSN 60
MRSTAIHNSAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSN
Sbjct: 1 MRSTAIHNSAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSN 60
Query: 61 VLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEE 120
VLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEE
Sbjct: 61 VLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEE 120
Query: 121 VTVESFIRVLTGRHHPATPRSKRLLTDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAY 180
VTVESFIRVLTGRHHPATPRSKRLLTDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAY
Sbjct: 121 VTVESFIRVLTGRHHPATPRSKRLLTDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAY 180
Query: 181 AIQTMFEDNRYHEMLVIADSCRSASMYEWIDSPNVLSLSSSLTHEESYSYDVDTDIGVYV 240
AIQTMFEDNRYHEMLVIADSCRSASMYEWIDSPNVLSLSSSLTHEESYSYDVDTDIGVYV
Sbjct: 181 AIQTMFEDNRYHEMLVIADSCRSASMYEWIDSPNVLSLSSSLTHEESYSYDVDTDIGVYV 240
Query: 241 IDRYTHYTVNFLTKEVKALNSSANMQDYIDSCPARKCLSNTGVRKDHYPKDVKRVRVTDF 300
IDRYTHYTVNFLTKEVKALNSSANMQDYIDSCPARKCLSNTGVRKDHYPKDVKRVRVTDF
Sbjct: 241 IDRYTHYTVNFLTKEVKALNSSANMQDYIDSCPARKCLSNTGVRKDHYPKDVKRVRVTDF 300
Query: 301 FGSSRIFQHLSEEIVLDDEFWA 322
FGSSRIFQHLSEEIVLDDEFWA
Sbjct: 301 FGSSRIFQHLSEEIVLDDEFWA 322
>ref|NP_195020.1| (NM_119448) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
pir||T05302 vacuolar processing enzyme (EC 3.4.22.-) isozyme gamma precursor -
Arabidopsis thaliana
emb|CAA21203.1| (AL031804) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
emb|CAB80011.1| (AL161582) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
gb|AAK43975.1|AF370160_1 (AF370160) putative gamma-VPE protein [Arabidopsis thaliana]
gb|AAL11612.1|AF424619_1 (AF424619) AT4g32940/F26P21_60 [Arabidopsis thaliana]
gb|AAL15210.1| (AY059104) putative gamma-VPE protein [Arabidopsis thaliana]
Length = 494
Score = 462 bits (1177), Expect = e-129
Identities = 61/307 (19%), Positives = 125/307 (39%), Gaps = 23/307 (7%)
Query: 9 SAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSI 68
P + FF ++ + WAVLV S ++NYRH +++ Y +
Sbjct: 33 DVIKLPSQASRFFR------PAENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLL 86
Query: 69 KRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIR 128
++ G+ + NI++ + +D+ N NPRPGT+ + G ++Y V DY G++V V++
Sbjct: 87 RKGGLKEENIVVFMYDDIANNYENPRPGTIINSPHGKDVY-QGVPKDYTGDDVNVDNLFA 145
Query: 129 VLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFE 187
V+ G S +++ + ++ I+ + HGG + S L DL ++
Sbjct: 146 VILGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHA 205
Query: 188 DNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGV 238
Y ++ ++C S S++E + + N+ + ++S E S+ +
Sbjct: 206 LGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYET 265
Query: 239 YVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARK--CLSNTGVRKDHYPKDVKRV 295
+ D Y+V ++ + L + Q Y S + + +
Sbjct: 266 CLGD---LYSVAWMEDSGMHNLQTETLHQQYELVKRRTAPVGYSYGSHVMQYGDVGISKD 322
Query: 296 RVTDFFG 302
+ + G
Sbjct: 323 NLDLYMG 329
>pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17078.1| (Z99956) asparagine-specific endopeptidase precursor [Phaseolus
vulgaris]
Length = 484
Score = 462 bits (1177), Expect = e-129
Identities = 64/295 (21%), Positives = 121/295 (40%), Gaps = 19/295 (6%)
Query: 24 RFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLA 83
R SG + V H WA+L S ++NYRH +++ Y +++ G+ D NII+ +
Sbjct: 34 RLPSDSGNGDNV--HGTRWAILFAGSSGYWNYRHQADICHAYQLLRKGGLKDENIIVFMY 91
Query: 84 EDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKR 143
+D+ NS NPR G + + G +Y V DY GE+VT +F L G T S +
Sbjct: 92 DDIAFNSENPRRGVIINSPNGDEVY-KGVPKDYTGEDVTAHNFYAALLGDKSKLTGGSGK 150
Query: 144 LL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCR 202
++ + ++ I+ + HGG + + DL ++ Y ++ ++C
Sbjct: 151 VVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYASDLNEVLKKKHASGTYKNLVFYLEACE 210
Query: 203 SASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGVYVIDRYTHYTVNFLT 253
S S++E + + NV + ++S E S+ + + D Y+V ++
Sbjct: 211 SGSIFEGLLPEDINVYATTASNADESSWGTYCPGEDPSPPPEYSTCLGD---LYSVAWME 267
Query: 254 -KEVKALNSSANMQDYIDSCPARKC--LSNTGVRKDHYPKDVKRVRVTDFFGSSR 305
+ L + Q Y L + + + + + G+
Sbjct: 268 DSDRHNLRTETLHQQYKLVKERTISGGLYYGSHVMQYGDVGLSKDILFHYLGTDP 322
>ref|NP_180165.1| (NM_128154) putative vacuolar processing enzyme [Arabidopsis
thaliana]
sp|P49047|VPEA_ARATH VACUOLAR PROCESSING ENZYME, ALPHA-ISOZYME PRECURSOR (ALPHA-VPE)
pir||T02629 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
gb|AAC31241.1| (AC004747) vacuolar processing enzyme, alpha-isozyme precursor
(alpha-VPE) [Arabidopsis thaliana]
dbj|BAA09614.2| (D61393) alpha-VPE [Arabidopsis thaliana]
gb|AAL24163.1| (AY058055) At2g25940/F17H15.3 [Arabidopsis thaliana]
Length = 478
Score = 461 bits (1176), Expect = e-129
Identities = 67/321 (20%), Positives = 130/321 (39%), Gaps = 30/321 (9%)
Query: 5 AIHNSAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLAL 64
A+ P FF + + WAVLV S ++NYRH ++V
Sbjct: 17 AVSGDVIKLPSLASKFFRP---------TENDDDSTKWAVLVAGSSGYWNYRHQADVCHA 67
Query: 65 YHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVE 124
Y +K+ G+ + NI++ + +D+ N NPRPG + + G ++Y + V DY G+EV V+
Sbjct: 68 YQLLKKGGVKEENIVVFMYDDIAKNEENPRPGVIINSPNGEDVY-NGVPKDYTGDEVNVD 126
Query: 125 SFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQ 183
+ + V+ G S +++ + ++ IY + HGG + S L DL ++
Sbjct: 127 NLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLK 186
Query: 184 TMFEDNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DT 234
+ Y ++ ++C S S++E + + N+ + ++S E S+ +
Sbjct: 187 KKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPS 246
Query: 235 DIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARK--CLSNTGVRKDHYPKD 291
+ + D Y+V ++ E L + + Y S +
Sbjct: 247 EYETCLGD---LYSVAWIEDSEKHNLQTETLHEQYELVKKRTAGSGKSYGSHVMEFGDIG 303
Query: 292 VKRVRVTDFFGSSRIFQHLSE 312
+ + ++ F G E
Sbjct: 304 LSKEKLVLFMG----TNPADE 320
>sp|Q39119|VPEG_ARATH VACUOLAR PROCESSING ENZYME, GAMMA-ISOZYME PRECURSOR (GAMMA-VPE)
dbj|BAA18924.1| (D61395) gamma-VPE [Arabidopsis thaliana]
Length = 490
Score = 461 bits (1176), Expect = e-129
Identities = 61/307 (19%), Positives = 125/307 (39%), Gaps = 23/307 (7%)
Query: 9 SAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSI 68
P + FF ++ + WAVLV S ++NYRH +++ Y +
Sbjct: 29 DVIKLPSQASRFFR------PAENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLL 82
Query: 69 KRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIR 128
++ G+ + NI++ + +D+ N NPRPGT+ + G ++Y V DY G++V V++
Sbjct: 83 RKGGLKEENIVVFMYDDIANNYENPRPGTIINSPHGKDVY-QGVPKDYTGDDVNVDNLFA 141
Query: 129 VLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFE 187
V+ G S +++ + ++ I+ + HGG + S L DL ++
Sbjct: 142 VILGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHA 201
Query: 188 DNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGV 238
Y ++ ++C S S++E + + N+ + ++S E S+ +
Sbjct: 202 LGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYET 261
Query: 239 YVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARK--CLSNTGVRKDHYPKDVKRV 295
+ D Y+V ++ + L + Q Y S + + +
Sbjct: 262 CLGD---LYSVAWMEDSGMHNLQTETLHQQYELVKRRTAPVGYSYGSHVMQYGDVGISKD 318
Query: 296 RVTDFFG 302
+ + G
Sbjct: 319 NLDLYMG 325
>pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17079.1| (Z99957) legumain-like proteinase precursor [Phaseolus vulgaris]
Length = 493
Score = 461 bits (1176), Expect = e-129
Identities = 64/320 (20%), Positives = 117/320 (36%), Gaps = 36/320 (11%)
Query: 15 LRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIP 74
+ +E WAVLV S + NYRH ++V Y + + G+
Sbjct: 33 NGKEQDSVIKLPTQEVDAE-SDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVK 91
Query: 75 DSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRH 134
+ NI++ + +D+ + NPRPG + G ++Y V DY GE VT +F VL G
Sbjct: 92 EENIVVFMYDDIATHELNPRPGVIINNPQGPDVYA-GVPKDYTGESVTSHNFFAVLLGDK 150
Query: 135 HPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHE 193
S +++ + + + +Y + HGG + + L +D ++ Y E
Sbjct: 151 SKVKGGSGKVINSKPEDRIFVYYSDHGGPGVLGMPNMPYLYAMDFIDVLKKKHASGGYKE 210
Query: 194 MLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDVD-------TDIGVYVIDRY 244
M++ ++C S S++E I N+ ++S E S+ + + D
Sbjct: 211 MVIYVEACESGSIFEGIMPKDLNIYVTTASNAQENSWGTYCPGMYPPPPPEYITCLGD-- 268
Query: 245 THYTVNFLT-KEVKALNSSANMQDYIDSCPARKC---LSNTGVRKDHYPKDVKRVRVTDF 300
Y+V ++ E L + Q Y + +
Sbjct: 269 -LYSVAWMEDSESHNLKKESVEQQYQSVKQRTSNFEAYAMGSHVMQYG------------ 315
Query: 301 FGSSRIFQHLSEEIVLDDEF 320
+E++ L F
Sbjct: 316 -----DANMTAEKLYLYHGF 330
>dbj|BAB29018.1| (AK013853) data source:SPTR, source key:O14822,
evidence:ISS~homolog to GPI TRANSAMIDASE~putative [Mus
musculus]
Length = 395
Score = 460 bits (1173), Expect = e-128
Identities = 154/285 (54%), Positives = 209/285 (73%), Gaps = 3/285 (1%)
Query: 36 TGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRP 95
+GHTNNWAVLVCTS+FWFNYRHV+N L++Y S+KRLGIPDS+I++MLA+D+ CN+RNP+P
Sbjct: 40 SGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNARNPKP 99
Query: 96 GTVYAARA-GTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKRLLTDHQSNVLI 154
TV++ + N+YG DVEVDYR TVE+F+RVLT P+TPRSKRLL+D +SN+LI
Sbjct: 100 ATVFSHKNMELNVYGDDVEVDYRSYRGTVENFLRVLTXEVPPSTPRSKRLLSDDKSNILI 159
Query: 155 YLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWIDSPN 214
Y+TGHGG+ F+KFQDSEE+TN++LA A + M++ RY+E+L I D+C+ ASMYE SPN
Sbjct: 160 YMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPN 219
Query: 215 VLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTKEVKALNSSANMQDYIDSCPA 274
+++L+SS E+S S+ D IGV+++DRYT Y + F + A S NM D CP
Sbjct: 220 IMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFWKEINPA--SQTNMNDLFQVCPK 277
Query: 275 RKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLSEEIVLDDE 319
C+S G R D + +D K V +TDFFGS R + +E+I L +
Sbjct: 278 SLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTEKISLQWD 322
>dbj|BAA84650.1| (AB025310) asparaginyl endopeptidase [Oryza sativa]
dbj|BAB85400.1| (AP003924) asparaginyl endopeptidase [Oryza sativa (japonica
cultivar-group)]
Length = 501
Score = 460 bits (1173), Expect = e-128
Identities = 69/319 (21%), Positives = 123/319 (37%), Gaps = 25/319 (7%)
Query: 6 IHNSAQFPPLRFRIFFGFRFERSSGL-------SEKVTGHTNNWAVLVCTSKFWFNYRHV 58
+ A P R + WAVL+ S ++NYRH
Sbjct: 23 LAAVAVARPRWEEEGSNLRLPSERAVAAGAAADDAAEAAEGTRWAVLIAGSNGYYNYRHQ 82
Query: 59 SNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRG 118
++V Y +KR G+ D NII+ + +D+ N NPRPG + G ++Y V DY G
Sbjct: 83 ADVCHAYQIMKRGGLKDENIIVFMYDDIAHNPENPRPGVIINHPQGGDVYA-GVPKDYTG 141
Query: 119 EEVTVESFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVD 177
+EV V++ VL G S ++L + ++ I+ + HGG + L D
Sbjct: 142 KEVNVKNLFAVLLGNKTAVKGGSGKVLDSGPNDHIFIFYSDHGGPGVLGMPTYPYLYGDD 201
Query: 178 LAYAIQTMFEDNRYHEMLVIADSCRSASMYEWID--SPNVLSLSSSLTHEESYSYDV--- 232
L ++ Y ++ ++C S S++E + NV + ++S E S+
Sbjct: 202 LVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNGINVYATTASNADESSWGTYCPGE 261
Query: 233 ----DTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKC---LSNTGVR 284
+ + D Y+V ++ +V L + + Q Y +
Sbjct: 262 YPSPPPEYDTCLGD---LYSVAWMEDSDVHNLRTESLKQQYNLVKERTSVQHTYYSGSHV 318
Query: 285 KDHYPKDVKRVRVTDFFGS 303
++ ++ V + GS
Sbjct: 319 MEYGSLELNAHHVFMYMGS 337
>ref|NP_176458.1| (NM_104948) beta-VPE [Arabidopsis thaliana]
gb|AAK56243.1|AF367254_1 (AF367254) At1g62710/F23N19_8 [Arabidopsis thaliana]
gb|AAL15381.1| (AY059156) At1g62710/F23N19_8 [Arabidopsis thaliana]
Length = 486
Score = 459 bits (1170), Expect = e-128
Identities = 66/306 (21%), Positives = 123/306 (39%), Gaps = 18/306 (5%)
Query: 16 RFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPD 75
RF E ++ + G WAVLV S + NYRH ++V Y +++ G+ +
Sbjct: 26 RFEPKILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKE 85
Query: 76 SNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHH 135
NI++++ +D+ + NPRPGT+ G ++Y V DY G VT +F VL G
Sbjct: 86 ENIVVLMYDDIANHPLNPRPGTLINHPDGDDVYA-GVPKDYTGSSVTAANFYAVLLGDQK 144
Query: 136 PATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEM 194
S +++ + ++ +Y HGG + ++ + D ++ Y EM
Sbjct: 145 AVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEM 204
Query: 195 LVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGVYVIDRYT 245
++ ++C S S++E I N+ ++S E SY ++ + D
Sbjct: 205 VIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGD--- 261
Query: 246 HYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLSN---TGVRKDHYPKDVKRVRVTDFF 301
Y+V ++ E L Q Y + ++ +K ++ +
Sbjct: 262 LYSVAWMEDSETHNLKKETIKQQYHTVKMRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQ 321
Query: 302 GSSRIF 307
G
Sbjct: 322 GFDPAT 327
>gb|AAK15049.1|AF238384_1 (AF238384) asparaginyl endopeptidase [Vigna radiata]
Length = 483
Score = 457 bits (1166), Expect = e-128
Identities = 58/291 (19%), Positives = 117/291 (39%), Gaps = 18/291 (6%)
Query: 24 RFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLA 83
R SG + V WA+L S ++NYRH +++ Y +++ G+ + NII+ +
Sbjct: 34 RLPSDSGNDDNV--KGTRWAILFAGSNGYWNYRHQADICHAYQILRKGGLKEENIIVFMY 91
Query: 84 EDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKR 143
+D+ N NPRPG + G ++Y V DY GE+ T +F L G T S +
Sbjct: 92 DDIAFNWDNPRPGVIINKPDGDDVY-EGVPKDYTGEDATAHNFYSALLGDKSALTGGSGK 150
Query: 144 LL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCR 202
++ + + I+ + HGG + + DL ++ Y ++ ++C
Sbjct: 151 VVNSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKNLVFYLEACE 210
Query: 203 SASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGVYVIDRYTHYTVNFLT 253
+ S++E + + N+ + ++S E S+ + + D Y+V ++
Sbjct: 211 AGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGD---LYSVAWME 267
Query: 254 -KEVKALNSSANMQDYIDSCPARKCLS-NTGVRKDHYPKDVKRVRVTDFFG 302
+ L + + Q Y + + + + + G
Sbjct: 268 DSDRHNLRTESLHQQYKVVKDRTLSGGWYGSHVMQYGDVEFSKDTLFLYLG 318
>dbj|BAA76745.1| (D89972) asparaginyl endopeptidase (VmPE-1A) [Vigna mungo]
Length = 482
Score = 457 bits (1164), Expect = e-127
Identities = 58/291 (19%), Positives = 117/291 (39%), Gaps = 18/291 (6%)
Query: 24 RFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLA 83
R SG + V WA+L S ++NYRH +++ Y +++ G+ + NII+ +
Sbjct: 33 RLPSDSGNDDNVQ--GTRWAILFAGSNGYWNYRHQADICHAYQILRKGGLKEENIIVFMY 90
Query: 84 EDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKR 143
+D+ N NPRPG + G ++Y V DY GE+ T +F L G T S +
Sbjct: 91 DDIAFNWDNPRPGVIINKPDGDDVY-EGVPKDYTGEDATAHNFYSALLGDKSALTGGSGK 149
Query: 144 LL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCR 202
++ + + I+ + HGG + + DL ++ Y ++ ++C
Sbjct: 150 VVSSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKNLVFYLEACE 209
Query: 203 SASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGVYVIDRYTHYTVNFLT 253
+ S++E + + N+ + ++S E S+ + + D Y+V ++
Sbjct: 210 AGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGD---LYSVAWME 266
Query: 254 -KEVKALNSSANMQDYIDSCPARKCLS-NTGVRKDHYPKDVKRVRVTDFFG 302
+ L + + Q Y + + + + + G
Sbjct: 267 DSDRHNLRTESLHQQYKVVKDRTLSGGWYGSHVMQYGDVEFSKDALFLYLG 317
>pir||S60049 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
Length = 478
Score = 456 bits (1163), Expect = e-127
Identities = 67/321 (20%), Positives = 131/321 (39%), Gaps = 30/321 (9%)
Query: 5 AIHNSAQFPPLRFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLAL 64
A+ P FF + + WAVLV S ++NYRH ++V
Sbjct: 17 AVSGDVIKLPSLASKFFRP---------TENDDDSTKWAVLVAGSSGYWNYRHQADVCHA 67
Query: 65 YHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVE 124
Y +K+ G+ + NI++++ +D+ N NPRPG + + G ++Y + V DY G+EV V+
Sbjct: 68 YQRLKKGGVKEENIVVLMYDDIAENEENPRPGVIINSPNGEDVY-NGVPKDYTGDEVNVD 126
Query: 125 SFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQ 183
+ + V+ G S +++ + ++ IY + HGG + S L DL ++
Sbjct: 127 NLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLK 186
Query: 184 TMFEDNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DT 234
+ Y ++ ++C S S++E + + N+ + ++S E S+ +
Sbjct: 187 KKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPS 246
Query: 235 DIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARK--CLSNTGVRKDHYPKD 291
+ + D Y+V ++ E L + + Y S +
Sbjct: 247 EYETCLGD---LYSVAWIEDSEKHNLQTETLHEQYELVKKRTAGSGKSYGSHVMEFGDIG 303
Query: 292 VKRVRVTDFFGSSRIFQHLSE 312
+ + ++ F G E
Sbjct: 304 LSKEKLVLFMG----TNPADE 320
>emb|CAC43295.1| (AJ309173) putative vacuolar processing enzyme [Beta vulgaris]
Length = 486
Score = 455 bits (1160), Expect = e-127
Identities = 65/308 (21%), Positives = 129/308 (41%), Gaps = 18/308 (5%)
Query: 15 LRFRIFFGFRFERSSGL--SEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLG 72
R + R+ WAVL+ S ++NYRH ++V Y +K+ G
Sbjct: 23 SRLMVDNLIRWPSDHPSIFESDDDSVGTRWAVLIAGSSGYWNYRHQADVCHAYQVLKKGG 82
Query: 73 IPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTG 132
+ D NII+ + +D+ + NPRPG + + G ++Y V DY GE+VTV +F + G
Sbjct: 83 LKDENIIVFMYDDIAYDEENPRPGVLINSPYGHDVYA-GVPKDYTGEDVTVNNFFAAILG 141
Query: 133 RHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRY 191
T S +++ + ++ I+ + HGG + L +L ++ Y
Sbjct: 142 NKDAITGGSGKVVNSGPNDHIFIFYSDHGGAGVLGMPTYPYLYADELIETLKEKHASGTY 201
Query: 192 HEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGVYVID 242
++V ++C S S++E I + N+ + ++S E S+ + + D
Sbjct: 202 KSLVVYIEACESGSIFEGILPEGLNIYATTASNAVESSWGTYCPGQDPNVPPEYDTCLGD 261
Query: 243 RYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLS-NTGVRKDHYPKDVKRVRVTDF 300
Y+V+++ E L++ + Q Y + K++ + + +
Sbjct: 262 ---LYSVSWIEDSERHNLHTESLKQQYEVVKTKTAEKPFYGSHVMQYGDKELTQDMLYLY 318
Query: 301 FGSSRIFQ 308
G++ +
Sbjct: 319 MGTNPNNE 326
>ref|NP_563825.1| (NM_100749) putative GPI-anchor transamidase (U32517) [Arabidopsis
thaliana]
Length = 388
Score = 455 bits (1159), Expect = e-127
Identities = 145/290 (50%), Positives = 199/290 (68%), Gaps = 6/290 (2%)
Query: 23 FRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMML 82
+ F S+G T HTNNWAVLVCTS+FWFNYRH++N L+LY ++KRLGIPD II+ML
Sbjct: 12 YSFVSSTG---DTTIHTNNWAVLVCTSRFWFNYRHMANTLSLYRTVKRLGIPDERIILML 68
Query: 83 AEDVPCNSRNPRPGTVYAA-RAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRS 141
A+D+ CN+RN P V+ NLYG +VEVDYRG EVTVE+F+RVLTGRH A PRS
Sbjct: 69 ADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRS 128
Query: 142 KRLLTDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSC 201
KRLL+D S++L+Y+TGHGGD F+KFQD+EEL + DLA A++ M E R+ E++++ D+C
Sbjct: 129 KRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTC 188
Query: 202 RSASMYEWIDSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTKEVKALNS 261
++A+++ + SP VL++ SSL E SYS+ +D+DIGV V+DR+T+YT+ F +
Sbjct: 189 QAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE--RLNIYD 246
Query: 262 SANMQDYIDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLS 311
+A++ S R +S R D Y + V VT+FFGS H
Sbjct: 247 NASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD 296
>pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme beta precursor -
Arabidopsis thaliana
dbj|BAA09615.1| (D61394) beta-VPE [Arabidopsis thaliana]
Length = 484
Score = 450 bits (1147), Expect = e-125
Identities = 67/304 (22%), Positives = 124/304 (40%), Gaps = 16/304 (5%)
Query: 16 RFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPD 75
RF E ++ + G WAVLV S + NYRH ++V Y +++ G+ +
Sbjct: 26 RFEPKILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKE 85
Query: 76 SNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHH 135
NI++++ +D+ + NPRPGT+ G ++Y V DY G VT +F VL G
Sbjct: 86 ENIVVLMYDDIANHPLNPRPGTLINHPDGDDVYA-GVPKDYTGSSVTAANFYAVLLGDQK 144
Query: 136 PATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEM 194
S +++ + ++ +Y HGG + ++ + D ++ Y EM
Sbjct: 145 AVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEM 204
Query: 195 LVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV-------DTDIGVYVIDRYT 245
++ ++C S S++E I N+ ++S E SY ++ + D
Sbjct: 205 VIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGD--- 261
Query: 246 HYTVNFLT-KEVKALNSSANMQDYIDSCPA-RKCLSNTGVRKDHYPKDVKRVRVTDFFGS 303
Y+V ++ E L Q Y + S ++ +K ++ + G
Sbjct: 262 LYSVAWMEDSETHNLKKETIKQQYHTMRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGF 321
Query: 304 SRIF 307
Sbjct: 322 EPAT 325
>gb|AAF45703.1| (AE003422) EG:133E12.3 gene product [Drosophila melanogaster]
Length = 322
Score = 439 bits (1120), Expect = e-122
Identities = 161/300 (53%), Positives = 212/300 (70%), Gaps = 3/300 (1%)
Query: 16 RFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPD 75
R + + + HTNNWAVLV S+FWFNYRHV+NVL++Y S+KRLGIPD
Sbjct: 17 RVEADNTSVLPEGFVDAAQRSTHTNNWAVLVDASRFWFNYRHVANVLSIYRSVKRLGIPD 76
Query: 76 SNIIMMLAEDVPCNSRNPRPGTVYAA-RAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRH 134
S II+M+A+D+ CN+RNPRPG VY N+YG DVEVDYRG EVTVE+F+R+LTGR
Sbjct: 77 SQIILMIADDMACNARNPRPGQVYNNANQHINVYGDDVEVDYRGYEVTVENFVRLLTGRT 136
Query: 135 HPATPRSKRLLTDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEM 194
T RSK+LL+D SNVLIYLTGHGGD F+KFQDSEE+T+ +LA IQ M+E RY+E+
Sbjct: 137 QNGTARSKKLLSDAGSNVLIYLTGHGGDGFLKFQDSEEITSQELADGIQQMWEKKRYNEL 196
Query: 195 LVIADSCRSASMYEWIDSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTK 254
+ D+C++AS+YE SPNVL+++SSL E+S S+ VD IGVY+IDRYT+Y + FL K
Sbjct: 197 FFMVDTCQAASLYEKFTSPNVLAVASSLVGEDSLSHHVDPSIGVYMIDRYTYYALEFLEK 256
Query: 255 EVKALNSSANMQDYIDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLSEEI 314
S + +++ CP R C+S GVRKD YP+D +V +TDFFG+ R + ++ I
Sbjct: 257 VQPF--SKRTIGEFLQVCPKRVCISTVGVRKDLYPRDPHKVPITDFFGAIRPTRVSTDRI 314
>pir||T13411 hypothetical protein 133E12.3 - fruit fly (Drosophila melanogaster)
emb|CAA15687.1| (AL009192) /prediction=(method:''genefinder'', version:''084'',
score:''46.36'')~/prediction=(method:''genscan'',
version:''1.0'',
score:''114.92'')~/match=(desc:''GPI-ANCHOR TRANSMIDASE
(EC 3.-.-.-)'', species:''SACCHAROMYCES CEREVISIAE
(BAKER'S YEAST>
Length = 326
Score = 439 bits (1120), Expect = e-122
Identities = 161/300 (53%), Positives = 212/300 (70%), Gaps = 3/300 (1%)
Query: 16 RFRIFFGFRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPD 75
R + + + HTNNWAVLV S+FWFNYRHV+NVL++Y S+KRLGIPD
Sbjct: 21 RVEADNTSVLPEGFVDAAQRSTHTNNWAVLVDASRFWFNYRHVANVLSIYRSVKRLGIPD 80
Query: 76 SNIIMMLAEDVPCNSRNPRPGTVYAA-RAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRH 134
S II+M+A+D+ CN+RNPRPG VY N+YG DVEVDYRG EVTVE+F+R+LTGR
Sbjct: 81 SQIILMIADDMACNARNPRPGQVYNNANQHINVYGDDVEVDYRGYEVTVENFVRLLTGRT 140
Query: 135 HPATPRSKRLLTDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEM 194
T RSK+LL+D SNVLIYLTGHGGD F+KFQDSEE+T+ +LA IQ M+E RY+E+
Sbjct: 141 QNGTARSKKLLSDAGSNVLIYLTGHGGDGFLKFQDSEEITSQELADGIQQMWEKKRYNEL 200
Query: 195 LVIADSCRSASMYEWIDSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTK 254
+ D+C++AS+YE SPNVL+++SSL E+S S+ VD IGVY+IDRYT+Y + FL K
Sbjct: 201 FFMVDTCQAASLYEKFTSPNVLAVASSLVGEDSLSHHVDPSIGVYMIDRYTYYALEFLEK 260
Query: 255 EVKALNSSANMQDYIDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLSEEI 314
S + +++ CP R C+S GVRKD YP+D +V +TDFFG+ R + ++ I
Sbjct: 261 VQPF--SKRTIGEFLQVCPKRVCISTVGVRKDLYPRDPHKVPITDFFGAIRPTRVSTDRI 318
>gb|AAF73260.1|AF154349_1 (AF154349) legumain [Rattus norvegicus]
Length = 435
Score = 427 bits (1088), Expect = e-118
Identities = 82/276 (29%), Positives = 133/276 (47%), Gaps = 13/276 (4%)
Query: 33 EKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRN 92
+ +W V+V S W+NYRH ++ Y I R GIPD II+M+ +D+ N N
Sbjct: 23 DDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIIVMMYDDIANNEEN 82
Query: 93 PRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATP-RSKRLL-TDHQS 150
P PG V GT++Y V DY GE+VT E+F+ VL G S ++L + +
Sbjct: 83 PTPGVVINRPNGTDVY-KGVPKDYTGEDVTPENFLAVLRGDEEAVKGKGSGKVLKSGPRD 141
Query: 151 NVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWI 210
+V +Y T HG + F + E+L DL I+ M+E Y +M+ ++C S SM +
Sbjct: 142 HVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRYMYEHKMYQKMVFYIEACESGSMMNHL 200
Query: 211 -DSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDY 268
D +V + +++ +E SY+ D + Y+ D Y+VN++ +V+ L + Y
Sbjct: 201 PDDIDVYATTAANPNESSYACYYDEERSTYLGDW---YSVNWMEDSDVEDLTKETLHKQY 257
Query: 269 IDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSS 304
+NT + K + ++V F G
Sbjct: 258 HLVK----SHTNTSHVMQYGNKSISTMKVMQFQGMK 289
>ref|NP_071562.1| (NM_022226) protease, cysteine, 1 (legumain) [Rattus norvegicus]
dbj|BAA84750.1| (AB032766) legumain [Rattus norvegicus]
Length = 435
Score = 427 bits (1088), Expect = e-118
Identities = 82/276 (29%), Positives = 133/276 (47%), Gaps = 13/276 (4%)
Query: 33 EKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRN 92
+ +W V+V S W+NYRH ++ Y I R GIPD II+M+ +D+ N N
Sbjct: 23 DDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIIVMMYDDIANNEEN 82
Query: 93 PRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATP-RSKRLL-TDHQS 150
P PG V GT++Y V DY GE+VT E+F+ VL G S ++L + +
Sbjct: 83 PTPGVVINRPNGTDVY-KGVPKDYTGEDVTPENFLAVLRGDEEAVKGKGSGKVLKSGPRD 141
Query: 151 NVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWI 210
+V +Y T HG + F + E+L DL I+ M+E Y +M+ ++C S SM +
Sbjct: 142 HVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRYMYEHKMYQKMVFYIEACESGSMMNHL 200
Query: 211 -DSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDY 268
D +V + +++ +E SY+ D + Y+ D Y+VN++ +V+ L + Y
Sbjct: 201 PDDIDVYATTAANPNESSYACYYDEERSTYLGDW---YSVNWMEDSDVEDLTKETLHKQY 257
Query: 269 IDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSS 304
+NT + K + ++V F G
Sbjct: 258 HLVK----SHTNTSHVMQYGNKSISTMKVMQFQGMK 289
>ref|NP_035305.1| (NM_011175) protease, cysteine, 1; preprolegumain [Mus musculus]
emb|CAA04439.1| (AJ000990) legumain [Mus musculus]
gb|AAF21659.1|AF044266_1 (AF044266) preprolegumain; cysteine Protease [Mus musculus]
Length = 435
Score = 427 bits (1087), Expect = e-118
Identities = 83/280 (29%), Positives = 133/280 (46%), Gaps = 13/280 (4%)
Query: 29 SGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPC 88
+ +W V+V S W+NYRH ++ Y I R GIPD II+M+ +D+
Sbjct: 19 PVGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIIVMMYDDIAN 78
Query: 89 NSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATP-RSKRLL-T 146
+ NP PG V GT++Y V DY GE+VT E+F+ VL G S ++L +
Sbjct: 79 SEENPTPGVVINRPNGTDVY-KGVLKDYTGEDVTPENFLAVLRGDAEAVKGKGSGKVLKS 137
Query: 147 DHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASM 206
+ +V IY T HG + F + ++L DL I+ M+E Y +M+ ++C S SM
Sbjct: 138 GPRDHVFIYFTDHGATGILVFPN-DDLHVKDLNKTIRYMYEHKMYQKMVFYIEACESGSM 196
Query: 207 YEWI-DSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLT-KEVKALNSSAN 264
+ D NV + +++ E SY+ D + G Y+ D Y+VN++ +V+ L
Sbjct: 197 MNHLPDDINVYATTAANPKESSYACYYDEERGTYLGDW---YSVNWMEDSDVEDLTKETL 253
Query: 265 MQDYIDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSS 304
+ Y +NT + K + ++V F G
Sbjct: 254 HKQYHLVK----SHTNTSHVMQYGNKSISTMKVMQFQGMK 289
>dbj|BAA09530.1| (D55696) cysteine protease [Homo sapiens]
Length = 433
Score = 424 bits (1081), Expect = e-118
Identities = 83/278 (29%), Positives = 133/278 (46%), Gaps = 13/278 (4%)
Query: 33 EKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRN 92
+ +WAV+V S W+NYRH ++ Y I R GIPD I++M+ +D+ + N
Sbjct: 21 DDPEDGGKHWAVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDN 80
Query: 93 PRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATP-RSKRLL-TDHQS 150
P PG V GT++Y V DY GE+VT ++F+ VL G S ++L + Q
Sbjct: 81 PTPGIVINRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQD 139
Query: 151 NVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWI 210
+V IY T HG + F + E+L DL I M++ Y +M+ ++C S SM +
Sbjct: 140 HVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHL 198
Query: 211 -DSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDY 268
D+ NV + +++ E SY+ D Y+ D Y+VN++ +V+ L + Y
Sbjct: 199 PDNINVYATTAANPRESSYACYYDEKRSTYLGDW---YSVNWMEDSDVEDLTKETLHKQY 255
Query: 269 IDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRI 306
+NT + K + ++V F G R
Sbjct: 256 HLVK----SHTNTSHVMQYGNKTISTMKVMQFQGMKRK 289
>pir||T00731 hypothetical protein F22O13.26 - Arabidopsis thaliana
Length = 428
Score = 423 bits (1079), Expect = e-117
Identities = 136/294 (46%), Positives = 191/294 (64%), Gaps = 10/294 (3%)
Query: 23 FRFERSSGLSEKVTGHTNNWAVLVCTSKFW----FNYRHVSNVLALYHSIKRLGIPDSNI 78
+ F S+G T HTNNWAVLVCTS+F + ++L + ++KRLGIPD I
Sbjct: 12 YSFVSSTG---DTTIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKRLGIPDERI 68
Query: 79 IMMLAEDVPCNSRNPRPGTVYAA-RAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPA 137
I+MLA+D+ CN+RN P V+ NLYG +VEVDYRG EVTVE+F+RVLTGRH A
Sbjct: 69 ILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENA 128
Query: 138 TPRSKRLLTDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVI 197
PRSKRLL+D S++L+Y+TGHGGD F+KFQD+EEL + DLA A++ M E R+ E++++
Sbjct: 129 VPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIM 188
Query: 198 ADSCRSASMYEWIDSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTKEVK 257
D+C++A+++ + SP VL++ SSL E SYS+ +D+DIGV V+DR+T+YT+ F
Sbjct: 189 VDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE--RL 246
Query: 258 ALNSSANMQDYIDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLS 311
+ +A++ S R +S R D Y + V VT+FFGS H
Sbjct: 247 NIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD 300
>ref|XP_007399.4| (XM_007399) legumain [Homo sapiens]
Length = 401
Score = 423 bits (1078), Expect = e-117
Identities = 82/278 (29%), Positives = 132/278 (46%), Gaps = 13/278 (4%)
Query: 33 EKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRN 92
+ +W V+V S W+NYRH ++ Y I R GIPD I++M+ +D+ + N
Sbjct: 21 DDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDN 80
Query: 93 PRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATP-RSKRLL-TDHQS 150
P PG V GT++Y V DY GE+VT ++F+ VL G S ++L + Q
Sbjct: 81 PTPGIVINRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQD 139
Query: 151 NVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWI 210
+V IY T HG + F + E+L DL I M++ Y +M+ ++C S SM +
Sbjct: 140 HVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHL 198
Query: 211 -DSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDY 268
D+ NV + +++ E SY+ D Y+ D Y+VN++ +V+ L + Y
Sbjct: 199 PDNINVYATTAANPRESSYACYYDEKRSTYLGDW---YSVNWMEDSDVEDLTKETLHKQY 255
Query: 269 IDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRI 306
+NT + K + ++V F G R
Sbjct: 256 HLVK----SHTNTSHVMQYGNKTISTMKVMQFQGMKRK 289
>gb|AAH03061.1|AAH03061 (BC003061) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 422 bits (1076), Expect = e-117
Identities = 82/278 (29%), Positives = 132/278 (46%), Gaps = 13/278 (4%)
Query: 33 EKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRN 92
+ +W V+V S W+NYRH ++ Y I R GIPD I++M+ +D+ + N
Sbjct: 21 DDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDN 80
Query: 93 PRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATP-RSKRLL-TDHQS 150
P PG V GT++Y V DY GE+VT ++F+ VL G S ++L + Q
Sbjct: 81 PTPGIVINRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQD 139
Query: 151 NVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWI 210
+V IY T HG + F + E+L DL I M++ Y +M+ ++C S SM +
Sbjct: 140 HVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHL 198
Query: 211 -DSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDY 268
D+ NV + +++ E SY+ D Y+ D Y+VN++ +V+ L + Y
Sbjct: 199 PDNINVYATTAANPRESSYACYYDEKRSTYLGDW---YSVNWMEDSDVEDLTKETLHKQY 255
Query: 269 IDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRI 306
+NT + K + ++V F G R
Sbjct: 256 HLVK----SHTNTSHVMQYGNKTISTMKVMQFQGMKRK 289
>ref|NP_005597.1| (NM_005606) legumain; protease, cysteine, 1 (legumain) [Homo
sapiens]
sp|Q99538|LEGU_HUMAN Legumain precursor (Asparaginyl endopeptidase)
emb|CAA70989.1| (Y09862) legumain [Homo sapiens]
gb|AAH08004.1|AAH08004 (BC008004) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 422 bits (1075), Expect = e-117
Identities = 82/278 (29%), Positives = 132/278 (46%), Gaps = 13/278 (4%)
Query: 33 EKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRN 92
+ +W V+V S W+NYRH ++ Y I R GIPD I++M+ +D+ + N
Sbjct: 21 DDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDN 80
Query: 93 PRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATP-RSKRLL-TDHQS 150
P PG V GT++Y V DY GE+VT ++F+ VL G S ++L + Q
Sbjct: 81 PTPGIVINRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQD 139
Query: 151 NVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWI 210
+V IY T HG + F + E+L DL I M++ Y +M+ ++C S SM +
Sbjct: 140 HVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHL 198
Query: 211 -DSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDY 268
D+ NV + +++ E SY+ D Y+ D Y+VN++ +V+ L + Y
Sbjct: 199 PDNINVYATTAANPRESSYACYYDEKRSTYLGDW---YSVNWMEDSDVEDLTKETLHKQY 255
Query: 269 IDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRI 306
+NT + K + ++V F G R
Sbjct: 256 HLVK----SHTNTSHVMQYGNKTISTMKVMQFQGMKRK 289
>emb|CAB42651.1| (AJ238881) putative preprolegumain [Nicotiana tabacum]
Length = 419
Score = 422 bits (1074), Expect = e-117
Identities = 58/277 (20%), Positives = 110/277 (38%), Gaps = 19/277 (6%)
Query: 57 HVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDY 116
H ++V Y +KR G+ D NI++ + +D+ + NPRPG + G+++Y V DY
Sbjct: 1 HQADVCHAYQILKRGGLKDENIVVFMYDDIAKSELNPRPGVIINHPNGSDVYA-GVPKDY 59
Query: 117 RGEEVTVESFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTN 175
GE VT + VL G S +++ + + +Y + HGG + + L
Sbjct: 60 TGEHVTAANLYAVLLGDKSAVKGGSGKIVDSKPNDRIFLYYSDHGGPGVLGMPNMPFLYA 119
Query: 176 VDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDVD 233
D ++ Y EM++ ++C S S++E + + N+ ++S E S+
Sbjct: 120 KDFIEVLKKKHAAGTYKEMVLYIEACESGSVFEGMMPEDLNIYVTTASNAEESSWGTYCP 179
Query: 234 -------TDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCLSN---TG 282
+ + D Y+V ++ E L Q Y +N
Sbjct: 180 GMDPPPPPEYITCLGD---LYSVAWMEDSESHNLKKETIKQQYEKVKERTSNFNNYNAGS 236
Query: 283 VRKDHYPKDVKRVRVTDFFGSSRIF-QHLSEEIVLDD 318
++ K++K +V + G + +I
Sbjct: 237 HVMEYGSKEIKPEKVYLYQGFDPATANLSANKIAFAH 273
>ref|NP_010618.1| (NC_001136) Protein involved in the attachment of
glycosylphosphatidylinositol (GPI) anchors to proteins;
Gpi8p [Saccharomyces cerevisiae]
sp|P49018|GPI8_YEAST GPI-ANCHOR TRANSAMIDASE
pir||S59796 probable membrane protein YDR331w - yeast (Saccharomyces
cerevisiae)
gb|AAB64766.1| (U32517) Ydr331wp [Saccharomyces cerevisiae]
Length = 411
Score = 418 bits (1065), Expect = e-116
Identities = 142/285 (49%), Positives = 196/285 (67%), Gaps = 3/285 (1%)
Query: 30 GLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCN 89
E + +TNNWAVLV TS+FWFNYRH++NVL++Y ++KRLGIPDS II+ML++DV CN
Sbjct: 27 AAHEVIATNTNNWAVLVSTSRFWFNYRHMANVLSMYRTVKRLGIPDSQIILMLSDDVACN 86
Query: 90 SRNPRPGTVYAAR-AGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKRLLTDH 148
SRN PG+V+ + +LYG VEVDYRG EVTVE+FIR+LT R P+SKRLLTD
Sbjct: 87 SRNLFPGSVFNNKDHAIDLYGDSVEVDYRGYEVTVENFIRLLTDRWTEDHPKSKRLLTDE 146
Query: 149 QSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYE 208
SN+ IY+TGHGGD F+KFQD+EE+ + D+A A Q M+E RY+E+ + D+C++ +MY
Sbjct: 147 NSNIFIYMTGHGGDDFLKFQDAEEIASEDIADAFQQMYEKKRYNEIFFMIDTCQANTMYS 206
Query: 209 WIDSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTKEVKALNSSANMQDY 268
SPN+L++ SS E SYS+ D +IGV VIDR+T+Y ++FL + K S+ +QD
Sbjct: 207 KFYSPNILAVGSSEMDESSYSHHSDVEIGVAVIDRFTYYCLDFLEQIDKN--STLTLQDL 264
Query: 269 IDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLSEE 313
DS K S+ GVR D + ++ V +TDFF + + +
Sbjct: 265 FDSFTFEKIHSHVGVRTDLFDRNPSEVLITDFFANVQNVIPDDSK 309
>dbj|BAB69947.1| (AB060129) legumain [Bos taurus]
Length = 433
Score = 416 bits (1059), Expect = e-115
Identities = 77/276 (27%), Positives = 130/276 (46%), Gaps = 13/276 (4%)
Query: 33 EKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRN 92
E +W V+V S W+NYRH ++ Y + R GIPD II+M+ +D+ + N
Sbjct: 21 EDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIVHRNGIPDEQIIVMMYDDIANSEDN 80
Query: 93 PRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATP-RSKRLL-TDHQS 150
P PG V G+++Y V DY GE+VT ++F+ VL G S ++L + +
Sbjct: 81 PTPGIVINRPNGSDVY-QGVLKDYTGEDVTPKNFLAVLRGDAEAVKGVGSGKVLKSGPRD 139
Query: 151 NVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWI 210
+V +Y T HG + F + E+L DL I+ M+E Y +M+ ++C S SM +
Sbjct: 140 HVFVYFTDHGATGILVFPN-EDLHVKDLNETIRYMYEHKMYQKMVFYIEACESGSMMNHL 198
Query: 211 -DSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDY 268
NV + +++ E SY+ D ++ D Y+VN++ +V+ L + Y
Sbjct: 199 PPDINVYATTAANPRESSYACYYDEQRSTFLGDW---YSVNWMEDSDVEDLTKETLHKQY 255
Query: 269 IDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSS 304
+NT + K + +++ F G
Sbjct: 256 QLVK----SHTNTSHVMQYGNKSISAMKLMQFQGLK 287
>pir||T40853 probable cysteine proteinase (EC 3.4.22.-) [similarity] - fission
yeast (Schizosaccharomyces pombe)
emb|CAB57844.1| (AL121783) putative gpi-anchor transamidase [Schizosaccharomyces
pombe]
emb|CAC13970.1| (AJ250428) GPI8p transamidase [Schizosaccharomyces pombe]
Length = 380
Score = 413 bits (1052), Expect = e-114
Identities = 145/281 (51%), Positives = 196/281 (69%), Gaps = 2/281 (0%)
Query: 35 VTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPR 94
+ HTNNWAVL+ TS+FWFNYRH +NVL +Y S+KRLGIPDS II+M+A+D CNSRN
Sbjct: 20 ESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNLF 79
Query: 95 PGTVY-AARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKRLLTDHQSNVL 153
PGTV+ A +LYG ++E+DY+G EVTVE+FIR+LT R TP SKRLLT+ +SN+L
Sbjct: 80 PGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPENTPASKRLLTNERSNIL 139
Query: 154 IYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWIDSP 213
IY+TGHGGD F+KFQD+EEL++ DLA AI+ + + RY+E+L + D+C++ S+Y I SP
Sbjct: 140 IYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIYSP 199
Query: 214 NVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTKEVKALNSSANMQDYIDSCP 273
NVL++ SS SYS+ D DIGV VIDR+T + FL V + S MQD I+S
Sbjct: 200 NVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLENRVDS-KSKLTMQDLINSYN 258
Query: 274 ARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLSEEI 314
+ S GV+ + + + +TDFFG+ R + SE+I
Sbjct: 259 PYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIELHSEKI 299
>gb|AAF99765.1|AC003981_15 (AC003981) F22O13.24 [Arabidopsis thaliana]
Length = 407
Score = 413 bits (1051), Expect = e-114
Identities = 138/290 (47%), Positives = 187/290 (63%), Gaps = 23/290 (7%)
Query: 23 FRFERSSGLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMML 82
+ F S+G T HTNNWAVLVCTS+FW ++KRLGIPD II+ML
Sbjct: 12 YSFVSSTG---DTTIHTNNWAVLVCTSRFW--------------TVKRLGIPDERIILML 54
Query: 83 AEDVPCNSRNPRPGTVYAA-RAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRS 141
A+D+ CN+RN P V+ NLYG +VEVDYRG EVTVE+F+RVLTGRH A PRS
Sbjct: 55 ADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRS 114
Query: 142 KRLLTDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSC 201
KRLL+D S++L+Y+TGHGGD F+KFQD+EEL + DLA A++ M E R+ E++++ D+C
Sbjct: 115 KRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTC 174
Query: 202 RSASMYEWIDSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTKEVKALNS 261
++A+++ + SP VL++ SSL E SYS+ +D+DIGV V+DR+T+YT+ F + N+
Sbjct: 175 QAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFERLNIYDNA 234
Query: 262 SANMQDYIDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLS 311
S N S R +S R D Y + V VT+FFGS H
Sbjct: 235 SLN-----RSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTD 279
>emb|CAB42655.1| (AJ238882) putative preprolegumain [Vicia narbonensis]
Length = 380
Score = 406 bits (1035), Expect = e-112
Identities = 49/257 (19%), Positives = 107/257 (41%), Gaps = 17/257 (6%)
Query: 60 NVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGE 119
+V Y +++ G+ + NII+ + +D+ + NPRPG + + G N+Y V DY GE
Sbjct: 1 DVCHAYQLLRKGGLKEENIIVFMYDDIAYSEENPRPGVIINSPHGENVY-EGVPKDYTGE 59
Query: 120 EVTVESFIRVLTGRHHPATPRSKRLL-TDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDL 178
+VTV +F L G + S +++ + + ++ + HGG + S + DL
Sbjct: 60 DVTVGNFFAALLGNKSALSGGSGKVVDSGPNDRIFVFYSDHGGPGVLGMPTSPYMYASDL 119
Query: 179 AYAIQTMFEDNRYHEMLVIADSCRSASMYEWI--DSPNVLSLSSSLTHEESYSYDV---- 232
++ Y ++ ++C S S++E + + N+ + +++ E S+
Sbjct: 120 VEVLKIKHAAGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTAANAEESSWGTYCPGEN 179
Query: 233 ---DTDIGVYVIDRYTHYTVNFLT-KEVKALNSSANMQDYIDSCPARKCL--SNTGVRKD 286
+ + D Y+V ++ ++ L + Q Y +
Sbjct: 180 PSPPPEYETCLAD---LYSVAWMEDSDIHNLQTETLHQQYELVKERTSNGNSNYGSHVMQ 236
Query: 287 HYPKDVKRVRVTDFFGS 303
+ ++ + + + GS
Sbjct: 237 YGDIELSKDSLFLYLGS 253
>ref|NP_188656.1| (NM_112912) vacuolar processing enzyme/asparaginyl endopeptidase,
putative [Arabidopsis thaliana]
dbj|BAB01880.1| (AP000383) vacuolar processing enzyme (proteinase) [Arabidopsis
thaliana]
Length = 466
Score = 401 bits (1020), Expect = e-110
Identities = 64/285 (22%), Positives = 132/285 (45%), Gaps = 16/285 (5%)
Query: 30 GLSEKVTGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCN 89
S + WAVLV S ++NYRH +++ Y +++ G+ D NII+ + +D+ +
Sbjct: 35 VESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQILRKGGLKDENIIVFMYDDIAFS 94
Query: 90 SRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKRLL-TDH 148
S NPRPG + G ++Y V DY E V V++F VL G T + +++ +
Sbjct: 95 SENPRPGVIINKPDGEDVY-KGVPKDYTKEAVNVQNFYNVLLGNESGVTGGNGKVVKSGP 153
Query: 149 QSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYE 208
N+ IY HG + +E+ D ++ M + +Y++M++ ++C S SM+E
Sbjct: 154 NDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRKKYNKMVIYVEACESGSMFE 213
Query: 209 WI--DSPNVLSLSSSLTHEESYS-------YDVDTDIGVYVIDRYTHYTVNFLT-KEVKA 258
I + N+ +++++ + E S+ ++IG + D + ++++L ++
Sbjct: 214 GILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIGTCLGDTF---SISWLEDSDLHD 270
Query: 259 LNSSANMQDYIDSCPARKC-LSNTGVRKDHYPKDVKRVRVTDFFG 302
++ Q Y + T + + + ++ + G
Sbjct: 271 MSKETLEQQYHVVKRRVGSDVPETSHVCRFGTEKMLKDYLSSYIG 315
CPU time: 97.94 user secs. 2.62 sys. secs 100.56 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.315 0.184 0.597
Gapped
Lambda K H
0.270 0.0645 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 252883702
Number of Sequences: 887402
Number of extensions: 15610614
Number of successful extensions: 23596
Number of sequences better than 10.0: 90
Number of HSP's better than 10.0 without gapping: 75
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 23113
Number of HSP's gapped (non-prelim): 107
length of query: 322
length of database: 277,845,442
effective HSP length: 56
effective length of query: 266
effective length of database: 228,150,930
effective search space: 60688147380
effective search space used: 60688147380
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.1 bits)
S2: 74 (32.8 bits)
